DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
·
2018
·
IF 2.913
Meng Chen,Mingmei Ding,Yuan Li,Xiaofang Zhong,Shuo Liu,Zheng Guo,Xiaoxue Yin,Shengli Fu,Jianmin Ye
Complement component 1q (C1q) is the initial protein of the classical complement pathway and plays an important role in immune response against bacterial infection. In this study, the full-length of C1q subcomponent A, B and C chain genes ( C1qA , C1qB and C1qC ) were identified and characterized from Nile tilapia ( Oreochromis niloticus ). Molecular characterization of these three C1q subcomponents ( OnC1qs ) harbored conserved amino acids through analyses of multiple sequence alignment and phylogenetic tree , which were homologous to other teleost species. Expression analysis revealed that the OnC1qs were highly expressed in liver. After the in vivo challenges of Streptococcus agalactiae and LPS , the mRNA expressions of OnC1qs were significantly up-regulated in liver. Meanwhile, the concentration variation of OnC1qs at the protein level from tilapia serum after challenge with S. agalactiae were measured by a competitive-inhibition ELISA . In addition, the up-regulation expressions of OnC1qs were also demonstrated in head kidney adherent leukocytes and the cell culture medium in vitro stimulation with S. agalactiae , Aeromonas hydrophila and LPS, respectively. Moreover, the recombinant OnC1qs enhanced expression of cytokines IL-6, IL-8 and IL-10 in head kidney adherent leukocytes, and were able to bind both heat-aggregated mouse IgG and IgM. Taken together, the results of this study indicated that OnC1qs might be involved in host defense against bacterial infection in Nile tilapia.
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 101
·99003-99009
·
IF 3.289
Yiran Liang,Peng Wang,Donghui Liu,Jing Zhan,Mai Luo,Jiajun Han,Xu Jing,Guojun Yao,Zhiqiang Zhou
To investigate the metabolic mechanism and enantioselectivity of the chiral herbicide quizalofop-ethyl and its primary chiral metabolite quizalofop-acid in animals by oral administration, the effects of the processes involved in digestion, absorption, transportation and metabolism, such as protein binding (pepsin, trypsin and serum albumin), intestinal absorption (everted gut sac), and degradation (plasma, liver microsome and cytosol) were studied in vitro. Protein binding experiments showed that quizalofop-ethyl interacted with pepsin, trypsin and serum albumin without enantioselectivity and the proteins did not stimulate metabolic processes that would produce quizalofop-acid. In the everted gut sac, quizalofop-ethyl was degraded rapidly to quizalofop-acid without enantioselectivity and the preferential absorption of (+)-quizalofop-acid was observed throughout the gut. In plasma, the metabolic process of quizalofop-ethyl was enantioselective with preferential degradation of (+)-quizalofop-ethyl and generation of (+)-quizalofop-acid. However there was no enantioselectivity in the degradation of quizalofop-ethyl or formation of quizalofop-acid in liver microsome. The metabolism of quizalofop-acid was nonenantioselective by liver microsome, sulfotransferase and glucuronosyltransferase. It could be inferred that the enzymes present in the lumen of intestine, blood and liver might facilitate the rapid transformation of quizalofop-ethyl to quizalofop-acid after oral administration, and the enantioselective absorption of quizalofop-acid enantiomers by intestine was the main reason for the enantioselectivity observed in rats in vivo.
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Shiju Wu,Cuicui Cai,Jing Cheng,Min Cheng,Hongbin Zhou,Jiali Deng
In order to highly enrich two liver cancer biomarkers (hexanal and 2-butanone) in human blood, in this study, natural nontoxic polydopamine/dialdehyde starch/chitosan (PD/DAS/CHI) coating material was synthesized and immobilized on the inner wall of polytetrafluoro-ethlyene (PTFE) tube. It was used to develop the method based on in-tube solid-phase microextraction (IT-SPME) with in-situ derivatization (ISD) coupled to high performance liquid chromatography for the determination of the above mentioned two liver cancer biomarkers in human blood. The simple, rapid and sensitive IT-SPME-ISD method can be finished within 11 min. Under optimum conditions, the limits of detection (LODs) were 1.4 and 1.6 nmol L −1 for hexanal and 2-butanone, respectively. The relative recoveries from real human blood samples were in the range from 70% to 91% with the intra- and inter-day precisions less than 7.2%. Furthermore, this method was successfully applied for the analysis of hexanal and 2-butanone in blood samples from healthy people with 0.42 ± 0.05 and 0.34 ± 0.04 μmol L −1 , while liver cancer patients with 1.90 ± 0.07 μmol L −1 and 0.91 ± 0.07 μmol L −1 , respectively. The t -test's results showed there is a statistically significant difference between the data from healthy persons and liver cancer patients. Hence, the developed method might be applied in the screening of suspected liver cancer patients.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL SCIENCES
·
2016
·
IF 2.641
Wei Weng,Peng Quan,Chao Liu,Hanqing Zhao,Liang Fang
The purpose of this work was to develop and design an appropriate drug-in-adhesive patch for transdermal delivery of risperidone (RISP). Various formulation factors were investigated by in vitro permeation study using excised rabbit skin. Increasing the drug concentration in the pressure sensitive adhesive (PSA) was used to enhance the drug permeation. To overcome the high crystallization tendency of the patch, several crystallization inhibitors such as PVP , PEG, and surfactants and fatty acids were evaluated by microscopy study. The mechanism of crystallization inhibition was investigated by differential scanning calorimetry , nuclear magnetic resonance spectrometer, and FT-IR studies. RISP and its active metabolite were determined after topical application of the optimized transdermal patch , and the in vivo pharmacokinetic parameters were compared with the intravenous administration group. The microscopy study indicated that fatty acid greatly inhibited the crystallization of RISP in PSA. The inhibition was attributed to the drug-additive interaction between amino group of RISP and the carboxyl group of fatty acid which was further confirmed by 1 H-NMR and FT-IR studies. The optimal permeation profile was obtained with the patches containing 5% RISP and 5% oleic acid in Duro-Tak ® 87-2287. The in vivo pharmacokinetic study exhibited a sustained absorption and metabolism profile and well correlated with the in vitro permeation data.
✓ 已验证
DOI
高影响因子
ACS Sensors
·
2017
·
IF 0
Shushu Ding,Sumei Cao,Yingzi Liu,Ying Lian,Anwei Zhu,Guoyue Shi
Sensitive and selective monitoring of sialic acid (SA) in cerebral nervous system is of great importance for studying the role that SA plays in the pathological process of Alzheimer’s disease (AD). In this work, we first reported an electrochemical biosensor based on a novel stimuli-responsive copolymer for selective and sensitive detection of SA in mouse brain. Notably, through synergetic hydrogen-bonding interactions, the copolymer could translate the recognition of SA into their conformational transition and wettability switch, which facilitated the access and enrichment of redox labels and targets to the electrode surface, thus significantly improving the detection sensitivity with the detection limit down to 0.4 pM. Besides amplified sensing signals, the proposed method exhibited good selectivity toward SA in comparison to potential interference molecules coexisting in the complex brain system due to the combination of high affinity between phenylboronic acid (PBA) and SA and the directional hydrogen-bonding interactions in the copolymer. The electrochemical biosensor with remarkable analytical performance was successfully applied to evaluate the dynamic change of SA level in live mouse brain with AD combined with in vivo midrodialysis. The accurate concentration of SA in different brain regions of live mouse with AD has been reported for the first time, which is beneficial for progressing our understanding of the role that SA plays in physiological and pathological events in the brain.
✓ 已验证
DOI
Clinical and Vaccine Immunology
·
2005
·Vol. 12
·期 11
·
IF 0
Yongjun Jiang,Hong Shang,Zining Zhang,Yingying Diao,Di Dai,Wenqing Geng,Min Zhang,Xiaoxu Han,Yanan Wang,Jing Liu
Natural killer (NK) cells, natural killer T (NKT) cells, and T lymphocytes were analyzed by using a flow cytometer in 225 human immunodeficiency virus (HIV)-positive individuals infected through the past sale of blood and plasma without receiving antiretroviral therapy in the People’s Republic of China. According to CD4 T-cell counts these HIV-infected adults were stratified into three groups: long-term slow progressors, HIV-infected subjects, and AIDS patients. NK cell counts in long-term slow progressors were higher compared to HIV infection and AIDS patients (P < 0.05) and lower compared to normal controls (P < 0.05), whereas NKT cell counts in slow progressors and the HIV infection group were not different from those of normal controls. NK cell counts in HIV-seropositive subjects were positively correlated with CD4 T-cell counts (P < 0.05), and NKT cell counts were positively correlated with CD4 T-cell and CD8 T-cell counts (P < 0.05). The CD8 T-cell counts were higher in slow progressors compared to those with HIV infection, AIDS patients, and normal controls. These results indicated that HIV infection causes alterations of NK cells and T cells in slow progressors, HIV-infected subjects, and AIDS patient groups, but no difference was found in NKT cell counts and percentages in slow progressors and the HIV-infected group compared to normal controls.
✓ 已验证
DOI
PubMed
WATER RESEARCH
·
2025
·
IF 12.4
Senlin Shao,Lei Zhu,Weiyi Li,Hongting Wan,Fanxi Zeng,Huarong Yu,Fangshu Qu
Organic fouling poses a primary barrier to the widespread application of membrane distillation (MD), an emerging technology for treating high‑salinity and chemically complex wastewaters. Despite its importance, quantitative insight into fouling dynamics remains limited, hindering the development of effective mitigation strategies. Here, we monitored the fouling dynamic using optical coherence tomography (OCT) and quantified this dynamic with an extended collision–attachment model. Results indicated that the model showed excellent agreement with experimental observations ( R 2 = 0.95), accurately capturing the kinetics of both foulant deposition and flux loss. With the model, we successfully identified and quantified the key factors governing the fouling dynamics. Notably, our analysis reveals that, in contrast to pressure-driven membrane processes where hydraulic resistance dominates, flux loss by organic fouling in MD is primarily governed by cake-enhanced temperature polarization. Based on these findings, we proposed a framework that enabled both qualitative analysis of organic fouling control strategies and quantitative description of their effects on fouling dynamics, and identified a self‑limiting regime in fouling dynamics, which was attributed to the feedback between collision-attachment kinetics and cake-enhanced temperature polarization effects. This work offers a theoretical basis for the optimization of MD processes and the development of fouling mitigation strategies.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DALTON TRANSACTIONS
·
2025
·
IF 3.3
Lihua Wang,Xionghongcan Zhou,Zengyu Ye,Sha Li,Yayang Wu,Kaiyue Song,Feng Zhao,Xianglong Li
Developing smart nanosystems that integrate controlled drug release with gas therapy represents a promising strategy to enhance chemotherapeutic efficacy while minimizing systemic toxicity. Herein, we report a biodegradable in-situ hydrogen sulfide (H2S) nanogenerator based on ZnS nanoparticles (NPs) for pH-responsive co-delivery of H2S and doxorubicin (DOX). ZnS NPs were synthesized via a rapid hydrothermal method and subsequently functionalized with hyaluronic acid (HA) to form ZnS@HA, which was then electrostatically loaded with DOX to yield ZnS@HA-DOX NPs. The nanosystem maintains structural integrity under physiological conditions (pH 7.4) but selectively releases both DOX and H2S under acidic tumor microenvironment (pH 5.0), as confirmed by fluorescence spectroscopy, lead acetate paper assays, and WSP-1 probe-based quantification. In vitro studies using 4T1 breast cancer cells revealed that ZnS@HA-DOX NPs exhibit significant cytotoxicity compared to ZnS@HA NPs alone, indicating a synergistic effect between H2S gas therapy and chemotherapy. This work demonstrates the potential of ZnS-based nanogenerators as a multifunctional platform for pH-triggered, site-specific, and synergistic cancer therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of the Taiwan Institute of Chemical Engineers
·
2026
·
IF 6.9
Wenjian Liu,Fei Wu,Yunyu Guo,Mengjiao Fan,Yan Tan,Yumeng Chen,Jiayue Zhu,Shu Zhang,Shuang Wang,Xun Hu
Background Aromatization is an important reaction route to form stable carbon skeleton for reserving pores generated during activation of biomass. Salts with Lewis acidity are known to catalyze dehydration and condensation reactions to form aromatic skeletons, while anions of salts are generally not inert and could influence evolution of pore development. Elucidation of the potential synergistic effects anions on pore evolution of activated carbon (AC) is of importance in optimizing the process for activation. Methods This study investigated the activation of peach wood with FeCl 3 , FeCl 2 , Fe 2 (SO 4 ) 3 or Fe(NO 3 ) 3 ) at 700°C, combined with in-situ IR spectroscopy to capture real-time dynamic change of reaction intermediates during activation process. Significant findings The results showed that Cl - in the iron salts uniquely stabilizes Fe 3+ (a distinctive advantage over other anions) for achieving efficient aromatization, yielding AC with the highest S BET (816.8 m 2 /g) and superior capability for removal of basic fuchsin (87.4%). FeCl 2 (Fe 2+ ) had weakened activity for aromatization, forming AC with loosely porous aggregates (597.6 m 2 /g). The activation with Fe 2 (SO 4 ) 3 reduced the sulfates and led to sulfur deposit blocking pores (S BET : 23.5 m 2 /g), with in-situ IR identifying S-O species that directly interfered with aromatization process. Fe(NO 3 ) 3 generated oxidative radicals that disrupted aromatization and wrapped Fe 3 O 4 species in inner structure, clogging pores (S BET : 230.8 m 2 /g). Increasing temperatures from 700 to 900°C in activation with FeCl 3 reduced the S BET progressively to 642.7 m 2 /g, resulting from excessive gasification, which also decreased AC yield to 17.4% and induced irreversible micropore-to-mesopore conversion, a temperature-dependent pore evolution pattern rarely highlighted in iron-salt activation systems. Notably, In-situ IR analysis revealed accelerated dehydration and aromatization at 300-400°C for consolidating microporous frameworks by Cl - and oxidation of carbonaceous intermediates by NO 3 - as well as formation of S-O species from SO 4 2- and their interference on aromatization. This work fills the knowledge gap in decoupling the roles of anions and iron valence in biomass activation, providing a targeted strategy for designing high-performance ACs with unique pore characteristics.
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2026
·
IF 3.9
Lemeng Wang,JiChao Hu,Jian Ma,Zedong Niu,YuChen Liu,Luanxiang Chao,Hemu Liu,Ruigeng Xie,Yuetong Zhao,Chenlu Mi,Kun Zhao,Dong Fu,Pengfei Qu,Pan Zhang
The industrial application of MEA-based carbon capture processes is significantly constrained by a low desorption rate and high energy consumption. To address these limitations, a NiCo@C catalyst was synthesized by using a hydrothermal-assisted impregnation method. The incorporation of Ni–Co dual doping into the carbon-based framework not only enhanced its stability and catalytic activity but also optimized its pore structure. A series of systematic characterizations were performed to evaluate the physicochemical properties of the material. The results indicate that the NiCo@C catalyst exhibits a substantially larger specific surface area and pore size compared to the Ni@C and Co@C catalysts. Additionally, the bimetallic catalysts introduce both weakly acidic and strongly acidic sites while maintaining a well-defined three-dimensional spherical structure. Compared to the noncatalytic MEA system, the introduction of NiCo@C increases the maximum desorption rate by 158% and the maximum desorption amount by 41.24%. These improvements contribute to a 53.48% reduction in energy consumption along with excellent cycle stability. The catalytic mechanism of NiCo@C was further investigated using 13C NMR, FT-IR, and Raman spectroscopy. The results reveal that bifunctional acid synergy in the solid NiCo@C catalyst promotes proton transfer and facilitates the breaking of C–N bonds, thereby enabling more efficient desorption with a lower energy consumption.
✓ 已验证
DOI
高影响因子
FASEB JOURNAL
·
2026
·Vol. 40
·期 4
·e71587
·
IF 4.3
Yiya Wang,Yuan Shi,Yafei Zhang,Jing Shi,Zihan Wang,Jie Li,Jie Li,Zhenhui Liu,Wenzheng Wu,Liyan Wang,Hongzhao Sun
Hydrogen sulfide (H 2 S), a gaseous neurotransmitter, plays critical roles in neuromodulation and autonomic regulation within the central nervous system (CNS). The supraoptic nucleus (SON), one of a number of hypothalamic regions governing neuroendocrine secretion, also projects to autonomic centers to modulate systemic functions. Despite evidence of H 2 S activity in other brain regions, its specific role in the SON, particularly in regulating gastric motility, remains unexplored. Physiological saline (PS), L-cysteine (L-Cys, 2 nmol and 4 nmol), sodium hydrosulfide (NaHS, 2 nmol and 4 nmol), and combinations of NaHS with protein kinase A (PKA) inhibitor (H-89), p38 mitogen-activated protein kinase (p38-MAPK) inhibitor (SB203580), or adenylate cyclase-cyclic adenosine monophosphate (AC-cAMP) inhibitor (SQ22536) were microinjected into the SON of male adult Wistar rats ( n = 5/group). Changes in gastric motility were detected and compared. The microinjection of either L-Cys or NaHS into the SON significantly inhibited gastric motility, with a greater inhibitory effect observed at the higher dose of each substance ( p < 0.01). However, when H-89, SB203580, and SQ22536 were injected prior to the administration of NaHS, no significant changes in gastric motility were observed, thus indicating that these inhibitors completely counteracted the inhibitory effect of NaHS on gastric motility. The microinjection of endogenous or exogenous H 2 S into the SON of rats inhibits gastric motility. This inhibitory effect may be mediated through the PKA, AC-cAMP, and p38-MAPK pathways. Graphical Hydrogen sulfide (H 2 S) delivered via microinjection of its endogenous precursor L-cysteine (L-Cys) or exogenous donor sodium hydrosulfide (NaHS) into the rat supraoptic nucleus (SON) exerts inhibitory effects on gastric motility. This regulatory effect is mediated through the PKA, AC-cAMP, and p38-MAPK signaling pathways and can be reversed by the respective pathway inhibitors (H-89, SQ22536, SB203580).
✓ 已验证
DOI
PubMed
高影响因子
Advanced Science
·
2026
·
IF 14.1
Lili Lian,Zhenmin Le,Xuanqiao Ye,Zimo Wang,Jiuxiao Li,Chenyu Dong,Yetao Shen,Jiazheng Li,Yueping Ren,Xiaoyin Ma,Wei Chen,Qinxiang Zheng
Hyperosmotic stress induced by tear film instability significantly exacerbates oxidative damage in corneal epithelial cells, contributing to the pathogenesis of dry eye disease (DED). Ferroptosis, an iron-dependent form of cell death driven by lipid peroxidation, has been identified as a critical downstream mechanism of oxidative damage in DED. However, its precise regulatory mechanisms remain unclear. In this study, we demonstrate that hyperosmotic stress promotes ferroptosis in corneal epithelial cells by downregulating the NAD-dependent deacetylase sirtuin 1 (SIRT1). SIRT1 positively regulates GPX4, a pivotal mediator of ferroptosis. Pharmacological activation of SIRT1 using SRT1720 alleviated oxidative damage and suppressed ferroptosis in corneal epithelial cells both in vitro and in vivo. Mechanistically, we further observed that SIRT1 deacetylates the HIF1α, stabilizing it via the ubiquitin-proteasome pathway. The SIRT1–HIF1α axis positively regulates GPX4 levels, thereby inhibiting ferroptosis activation. These results reveal a previously unrecognized pathway of ferroptosis regulation in DED and suggest a potential therapeutic strategy for reducing oxidative damage in corneal epithelium.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）