Frontiers in Microbiology
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2026
·
IF 5.8
Lin Jiang,Xiaofang Yan,Fang Zhang,Ailing Chen,Yixuan Chen,Yongbiao Zheng,Lingjun Yu
Introduction N -acetylglucosamine (GlcNAc) serves as a signaling molecule that triggers a variety of physiological responses in microorganisms. However, its regulatory roles in metabolite biosynthesis and siderophore production in Lysobacter sp. 3655 remain unclear, especially regarding the quorum sensing (QS) signal 4-hydroxybenzoic acid (4-HBA) and the siderophore lysochelin under iron-deficient conditions. This study aimed to explore the regulatory effects of GlcNAc on metabolite profiles and lysochelin biosynthesis in Lysobacter sp. 3655. Methods Lysobacter sp. 3655 was cultured in oligotrophic medium with GlcNAc induction. The induced differential metabolite was isolated and structurally identified by nuclear magnetic resonance and liquid chromatography-mass spectrometry. Gene deletion mutants of GlcNAc catabolic genes ( nagA , nagE2 ), and L-phenylalanine biosynthesis gene (pheA) were constructed, as well as multiple deletion mutants in the lenB2 (4-HBA biosynthetic gene) mutant background. Growth status and lysochelin yield of these mutants were evaluated under iron-deficient conditions. Exogenous complementation assays were performed using 4-HBA, GlcNAc, p -hydroxybenzaldehyde, cinnamic acid, and p-hydroxycinnamic acid to verify their functional effects. Results GlcNAc specifically induced the production of a unique metabolite in Lysobacter sp. 3655, which was identified as p -hydroxybenzaldehyde. Deletion of nagA , nagE2 , or pheA completely abolished GlcNAc-induced p -hydroxybenzaldehyde biosynthesis. The lenB2 mutant showed significant growth defects and remarkably decreased lysochelin production under iron limitation, while exogenous 4-HBA or GlcNAc restored both phenotypes. Further disruption of nagA , nagE2 , or pheA in the lenB2 mutant eliminated the complementation effect of GlcNAc on lysochelin production. p -hydroxybenzaldehyde restored lysochelin biosynthesis by converting to 4-HBA, and the L-phenylalanine catabolic intermediates cinnamic acid and p-hydroxycinnamic acid also rescued the growth and lysochelin production defects of the lenB2 mutant. Discussion Our results reveal that GlcNAc regulates two metabolic pathways in Lysobacter sp. 3655: it promotes p -hydroxybenzaldehyde production through GlcNAc catabolism and L-phenylalanine metabolism, and restores lysochelin biosynthesis in the QS-deficient lenB2 mutant via either 4-HBA converted from p -hydroxybenzaldehyde or alternative pathways mediated by L-phenylalanine catabolites. These findings uncover a novel regulatory network controlling lysochelin biosynthesis in Lysobacter sp. 3655, providing important references for investigating the biocontrol mechanisms of Lysobacter species.
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Advanced Science
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2026
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IF 14.1
Zhaohui Wang,Xiaojin Deng,Xujuan Huang,Yuhang Pan,Yao Qin,Lu Zhang,Le Xu,Tianyi Qin,Deteng Zhang,Yi Ma,Yalong Wang
The lack of selective antifungal agents remains a major clinical challenge. Here, we discover facet-polysaccharide matching between polyvinylpyrrolidone-directed tetragonal CuFeSe 2 nanocrystals (CFS-P) and fungi drives ultrapotent and highly selective fungicidal activity. High-index facets uniquely exposed on CFS-P show markedly enhanced affinity for mannan, the dominant polysaccharide of the fungal outer cell wall, as validated by DFT, molecular dynamics, and calorimetry. Mannan recognition promotes efficient fungal internalization of CFS-P and its preferential accumulation in vacuoles, leading to vacuolar cavitation, calcium overload, mitochondrial hyperpolarization, and transcriptional reprogramming of metal and vacuole-associated pathways. CFS-P achieves therapeutic efficacy comparable to amphotericin B while exhibiting superior mammalian biocompatibility. This work reveals a previously unrecognized wall-vacuole disruptive antifungal mechanism and enlightens the exploration of facet-encoded biological recognition in anti-infective design.
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Journal of Building Engineering
·
2026
·
IF 8.1
Xi Deng,Kaiyuan Li,Yongqiang Ye,Xinke Huo,Wei He,Fei Xiao
The inherent combustibility of wood represents a critical vulnerability, compromising the structural integrity and fire safety of wooden buildings. This work reports on a highly fire-safe P/N/B wood composite. This material was fabricated based on the freeze-thaw strategy, involving the co-impregnation of amino trimethylene phosphonic acid (ATMP) and boric acid (BA) into the wood substrate. Benefiting from the pore size changes caused by freeze-thaw cycles, the total loading of ATMP and ATMP/BA in wood increased by 37.5% and 21.9%, respectively, as compared the untreated sample. The freeze-thawed wood composite exhibited an ultra-high limiting oxygen index (LOI) of 87.9 % and a V-0 rating was reached in the UL-94 vertical burning test (5 mm). The peak heat release rate (pHRR) and total heat release rate (THR) decreased by 57% and 91%, respectively, as compared to untreated wood. The results showed that the improved fire retardancy performances were attributed to the char layer formed during the combustion, which acts as a thermal barrier, preventing the transfer of heat and mass. SEM, Raman, XPS, FTIR, and XRD results confirmed that phosphates and borophosphates species were formed during burning and acted as reinforcing substances to enhance the strength and stability of the char layer. In summary, this study provides a simple and effective approach for modifying the fire safety of wood, with great potential for construction applications.
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TRIBOLOGY INTERNATIONAL
·
2026
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IF 7.6
Jiajia Cen,Shuangqi Li,Kai Chen,Dekun Zhang
Polyetheretherketone (PEEK) possesses excellent corrosion resistance, moderate friction properties, and high‑temperature stability. However, under severe operating conditions, its coefficient of friction (COF) and wear rate increase significantly, limiting broader application. In this work, P‑MoS₂ was synthesized in situ on a PEEK matrix using a hydrothermal method, while K‑BN‑OH was prepared via surface grafting with a coupling agent. Both fillers were then blended with a PEEK/PTFE matrix to produce a novel PEEK/PTFE/P‑MoS₂/K‑BN‑OH composite. Orthogonal experiments identified the optimal composition of PEEK/PTFE/P‑MoS₂, which exhibited the best overall performance. The effect of K‑BN‑OH content on the mechanical, thermal, and tribological properties of the composites was systematically studied. Tribological behavior under varying conditions was analyzed to elucidate wear mechanisms and filler synergy. Results show that the composite with 25% PTFE and 5% P‑MoS₂ achieved the most balanced performance: elastic modulus (1414.62 MPa), compressive strength (127.81 MPa), COF (0.108), and wear rate (1.81 ×10⁻⁶ mm³/N·h) decreased by 22.58%, 35.82%, 73.13%, and 59.78%, respectively, compared to pure PEEK, while creep strain (2.77%) increased by 95.62%. Further addition of 3% K‑BN‑OH enhanced mechanical and thermal properties: elastic modulus rose to 1520.13 MPa, compressive strength to 131.33 MPa, creep strain fell to 1.82%, and thermal conductivity reached 0.294 W/m·K. The average coefficient of friction(ACOF) declined to 0.094, though wear rate rose slightly to 2.26 × 10⁻⁶ mm³/N·h. Tribological tests revealed that wear rate increased with rotational speed, while ACOF showed a non‑monotonic trend, first decreasing and then increasing. With increasing load, both ACOF and wear rate initially decreased before rising, reaching minima at 20 N (0.077 and 2.09 ×10⁻⁶ mm³/N·h, respectively). Molecular dynamics simulations elucidated that PTFE formed a lubricating film through interfacial enrichment, while MoS₂ enhanced matrix ordering and promoted interfacial slip via π-π stacking and C-S bonding. In contrast, h-BN disrupted the synergistic lubrication network established between PTFE and MoS₂ due to its spatial blocking effect. This work systematically elucidates the synergistic mechanisms of fillers from the microscopic level to macroscopic performance, providing a theoretical basis for the design of high‑performance PEEK composites tailored to specific operating conditions.
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CHEMICAL RESEARCH IN TOXICOLOGY
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2026
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IF 4.1
Naijie Wei,Xiaowen Yan,Mingyue Yu,Rong Li,Zhixin Xiao,Shi Chen,Limin Yang,Qiuquan Wang,Qian Liu,Guibin Jiang
N-(1,3-Dimethylbutyl)-N’-phenyl-p-phenylenediamine Quinone (6PPDQ) not only causes acute mortality in salmon but also induces toxicities in other living organisms. The electrophilic quinone moiety in the 6PPDQ molecular structure can participate in binding to the cysteine residues that ubiquitously exist in protein nucleophiles, which are responsible for its toxicities. Out of the 82 6PPDQ-bonded proteins found in the human model cell line A549 on the sulfhydryl-reactive proteomics platform, which enables the precise identification of covalent binding protein targets of a pollutant, we discovered three 6PPDQ-bonded mitochondrial proteins─NDUS6, COX5B, and ATP5PB─that are involved in mitochondrial dysfunction for the first time. They impede the function of mitochondria, as witnessed by the decreased enzymatic activities of mitochondrial respiratory chain Complexes I (27.63%) and IV (23.11%), the decreased cellular ATP content (19.94%), and the reduced mitochondrial membrane potential (3.2-fold), as well as the elevated mitochondrial ROS level (2.2-fold) under the environmentally relevant 8.9 μg/L 6PPDQ exposure compared to the controls. Our findings provide experimental evidence for elucidating 6PPDQ’s toxicities at the molecular level, and the knowledge learned will enhance the public’s awareness of the adverse impacts of environmental pollution on health.
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Surfaces and Interfaces
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2026
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IF 6
Balamurali Ravichandran,Naresh Narayanan,Narayanamoorthy Bhuvanendran,Huiyuan Liu,Weiqi Zhang,Huaneng Su
PtCo nanoparticles anchored on P,S,N-doped porous carbon nitride enables strong ORR activity. • Hierarchical porosity and heteroatom doping enhance Pt dispersion and mass transport. • PtCo/P,S-HPCN shows 2.37 mA cm ⁻2 Pt ⁻1 activity and superior ORR durability. • Outperforms commercial Pt/C in both RDE and single-cell PEMFC performance tests. • Robust carbon nitride support resists corrosion in acidic, high-potential environments. Maximize the Pt utilization with high durability and efficient mass transport is crucial on the electrocatalyst design for the practical low-temperature fuel cell applications. In this work, we designed an ultrafine PtCo alloy nanoparticles distributed uniformly on phosphorus and sulfur co-doped hierarchical porous carbon nitride (PtCo/P,S-HPCN), synthesized via hydrothermal treatment, pyrolysis, and chemical reduction. The heteroatom-doped carbon nitride framework exhibits superior chemical stability compared to conventional carbon materials, while its hierarchical porous structure facilitates uniform dispersion of ultrafine PtCo nanoparticles (∼1.8 nm) and accelerates reactant transport. Synergistic effects from graphitic/pyridinic nitrogen species, P/S doping, and Pt–Co alloying modulate the electronic structure of Pt, optimizing *OOH binding and enhancing ORR kinetics. The optimized catalyst delivers excellent performance in acidic media, achieving a high half-wave potential (0.88 V vs. RHE), a mass activity of 0.91 mA µgPt⁻ 1 , and a specific activity of 2.37 mA cmPt⁻ 2 at 0.85 V. Remarkably, it retains 73% of its initial mass activity and loses only 11 mV in half-wave potential after 5000 durability cycles. In practical PEMFC tests, the PtCo/P,S-HPCN cathode achieves a peak power density of 0.42 W cm⁻², surpassing commercial 40 wt% Pt/C. This study demonstrates a promising strategy for designing durable, low-Pt and high activity catalysts for PEMFC applications. Download: Download high-res image (217KB) Download: Download full-size image
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International Journal of Applied Ceramic Technology
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2026
·Vol. 23
·期 3
·e70215
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IF 2.6
Yu Fu,Sheng Jiang,Biao Wu,Xiaoli Deng,Xiaochun Wang
Ceramic metallization provides an effective insulation strategy for reversible solid oxide cell (RSOC) pipeline connections that are prone to side short-circuiting. In this work, 95 wt.% Al 2 O 3 ceramics were metallized by the activated Mo–Mn method, and 15 metallization formulations with different Mo, MnO, Al 2 O 3 , and SiO 2 contents were designed to optimize the microstructure, phase composition, and bonding strength of the metallized layer. The results show that, after sintering at 1400°C for 1 h, the optimal formulation consists of 70 wt.% Mo, 9 wt.% MnO, 6.3 wt.% Al 2 O 3 , and 14.7 wt.% SiO 2 . Under this condition, the Mo skeleton is continuous, the activator melt forms a glassy phase, and a reliable transition layer is developed at the ceramic/metallization interface, yielding a shear strength of 146.3 MPa. Electroless Ni–P post-plating on the optimized metallized surface produces a dense and continuous coating with typical nodular morphology and an average thickness of about 15–17 µm. The plated layer exhibits a relatively uniform hardness of 382.84 HV and maintains good structural integrity after 300 thermal-shock cycles, demonstrating promising potential for RSOC-related ceramic-to-metal insulating connection components.
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Energy Materials
·
2026
·Vol. 6
·期 8
·N
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IF 11.9
Jingjing Qie\u003Csup\u003E1,\u003C\u002Fsup\u003E,Huiyue Sun\u003Csup\u003E1,\u003C\u002Fsup\u003E,Shuang Geng\u003Csup\u003E1,\u003C\u002Fsup\u003E,Yongqi Shang\u003Csup\u003E1\u003C\u002Fsup\u003E,Qunzhi Huang\u003Csup\u003E1\u003C\u002Fsup\u003E,Jishi Wei\u003Csup\u003E1,2,\u003C\u002Fsup\u003E,Ke Chen\u003Csup\u003E1\u003C\u002Fsup\u003E,Taifeng Liu\u003Csup\u003E3\u003C\u002Fsup\u003E,Shuxi Dai\u003Csup\u003E1,\u003C\u002Fsup\u003E,Wuxing Hua\u003Csup\u003E1,4,\u003C\u002Fsup\u003E,Zhen Zhou\u003Csup\u003E4\u003C\u002Fsup\u003E
Developing an effective electrocatalyst to regulate the kinetics of the sulfur redox reaction is essential for inhibiting polysulfide shuttling in Li-S batteries. Here, the electronic structure of CoTe, modulated by cation doping, is systematically investigated to elucidate how orbital hybridization optimizes sulfur redox electrocatalysis. Among the cation-doped CoTe compounds, the introduction of Ni dopants results in the most significant shift of the p-band center of CoTe (Ni-CoTe) towards the Fermi energy level, which facilitates the formation of numerous Te vacancies on the CoTe surface. This structural configuration endows CoTe with a multitude of active sites that bind polysulfide intermediates, lowering the activation energy (Ea) of the sulfur reduction reaction, specifically reducing the Ea for the conversion of lithium polysulfides to Li2S from 0.73 eV to 0.65 eV. As a result, the Li-S battery with Ni-CoTe catalyst delivers exceptional cyclability, with a low-capacity decay of 0.049% per cycle over 1,000 cycles at 2.0 C. Moreover, an initial areal capacity of 13.5 mAh cm-2 at 0.02 C is achieved with a high sulfur loading (10.0 mg cm-2) and a low electrolyte-to-sulfur ratio (5.0 µL mg-1). These findings will provide fundamental insights into orbital-level catalyst design principles for advanced sulfur electrochemistry.
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Waste and Biomass Valorization
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2026
·
IF 3.1
Huang Yue,Li Lifeng,Ruan Tao,Ouyang Xinping
The catalytic conversion of lignin derivatives into specific chemicals represents a key pathway for achieving high-valued utilization of biomass. A new Ag–Co 0 /Fe 0.04 Co 0.96 O catalyst was constructed for the selective demethoxylation of vanillic acid derived from lignin depolymerization to produce p-Hydroxybenzoic acid. Compared to Ag–Co 0 /CoO, Fe doping suppressed Co reduction, endowing the Ag–Co 0 /Fe 0.04 Co 0.96 O catalyst with suitable hydrogenation activity and more abundant acidic sites. The hydrogen spillover effect inhibited decarboxylation, dehydroxylation, and benzene ring hydrogenation during the demethoxylation reaction. Oxyphilic Fe species changed the adsorption geometry of vanillic acid from parallel adsorption into twist configuration, avoiding the hydrogenation of benzene ring, and thereby improving the demethoxylation selectivity to p-Hydroxybenzoic acid. By optimizing catalyst preparation and reaction conditions to minimize decarboxylation and over-hydrogenation byproducts, the Ag–Co 0 /Fe 0.04 Co 0.96 O catalyst contributed to a 98.52% conversion of vanillic acid and a 89.62% selectivity to p-Hydroxybenzoic acid under 0.4 MPa H 2 , 0.4 MPa CO 2 at 220 °C for 2 h. This research provided a theoretical foundation and practical guidance for the catalytic demethoxylation of phenolic acids, offering new insights into high-valued utilization pathways for biomass. Graphical
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2026
·
IF 9.6
Jinting Chen,Jiayi Shang,Qing Chang,Long Wang,Ruiqi Yang,Han Wang,Jing Zhou,Yu Cao
Sodium metal batteries (SMBs) are regarded as compelling energy storage systems owing to their high theoretical capacity and the abundance of sodium (Na). However, SMBs with cycling stability under extreme climatic conditions remain highly challenging, due to the instability of the solid electrolyte interphase (SEI) and dendrite growth, which is further aggravated when operating under low negative-to-positive capacity ratio (N/P) conditions. Herein, a high-energy-density and wide-temperature SMB is realized using a hetero-salt-regulated electrolyte strategy. Specifically, lithium difluorophosphate (LiDFP) is introduced as a hetero-salt additive into a dual-salt ether electrolyte to stabilize the Na metal anodes. Li + competitively coordinates with solvent molecules, causing the Na + solvation structure to shift toward an anion-rich configuration. The formed anion-dominated solvation shell promotes the formation of a mechanically robust and electronically insulating LiF/NaF-rich SEI, which in turn suppresses interfacial side reactions and effectively mitigates dendrite growth. Consequently, the assembled Na||Na 3 V 2 (PO 4 ) 2 F 3 (NVPF) SMBs (N/ P = 4:1) achieve a high energy density of 335.90 Wh kg −1 (with power density of 26,650.00 W kg −1 ) at 25 °C (based on total mass of the active materials on anode and cathode) and maintain stable operation across a wide temperature range of −40 to 45 °C. This work expedites the development of SMBs for energy-intensive applications in extreme environments. Download: Download high-res image (228KB) Download: Download full-size image
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BIOMASS & BIOENERGY
·
2026
·
IF 7
Wenjun Ying,Xudong Li,Sisi Wang,Junhua Zhang,Qiang Yong
Low-dose thiourea enhanced p -TsOH delignification of bamboo by 24.6%. • Thiourea increased total β- O -4 retention from 35.6% to 79.3%. • Thiourea improved the glucose yield from 71.4% to 87.5%. • Enzymatic hydrolysis residue was upgraded into S-doped HC and S/N-doped CQDs. Acid pretreatment improves cellulose accessibility and promotes lignin removal, but reactive benzylic intermediates often induce lignin condensation and redeposition, limiting enzymatic hydrolysis and lignin valorization. In this work, low-dosage thiourea was introduced into a p -toluenesulfonic acid ( p -TsOH) system to nucleophilically stabilize dissolved and residual lignin intermediates while introducing S/N functionalities. The addition of thiourea increased the lignin removal from 62.1% for PTB to 77.4% for PTSN1%B and the glucose yield from 71.4% to 87.5%, while increasing total β-O-4 retention of recovered lignin from 35.6% to 79.3%. After enzymatic hydrolysis, the lignin-rich residue was converted by one-pot hydrothermal treatment into hydrochar and carbon quantum dots. The introduction of S functionalities into hydrochar increased the maximum methylene blue adsorption capacity from 87.6 mg/g for HC-C to 115.1 mg/g for HC-SN while the incorporation of S/N functionalities into carbon quantum dots enhanced blue-green fluorescence intensity from 100000 to 400000 a. u. This work provides an integrated route for suppressing lignin condensation, improving carbohydrate conversion, and upgrading enzymatic hydrolysis residue into functional carbon materials in a bamboo biorefinery. Download: Download high-res image (411KB) Download: Download full-size image
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Materials
·
2025
·Vol. 18
·期 13
·3065
·
IF 3.2
Wenjie Wu,Wenxuan Guo,Zeyang Liu,Chenxi Zhang,Aobing Li,Caihua Su,Chunxia Wang
Developing efficient and durable electrocatalysts for the alkaline hydrogen evolution reaction (HER) is crucial for sustainable hydrogen production. Herein, we report a novel RuP2/Mn2P2O7heterojunction anchored on a three-dimensional nitrogen and phosphorus co-doped porous carbon (RuP2/Mn2P2O7/NPC) framework as a high-performance HER catalyst, synthesized via a controlled pyrolysis–phosphidation strategy. The heterostructure achieves uniform dispersion of ultrafine RuP2/Mn2P2O7heterojunctions with well-defined interfaces. Furthermore, phosphorus doping restructures the electronic configuration of Mn and Ru species at the RuP2/Mn2P2O7heterointerface, enabling enhanced catalytic activity through the accelerated electron transfer and kinetics of the HER. This RuP2/Mn2P2O7/NPC catalyst exhibits exceptional HER activity with 1 M KOH, requiring only 69 mV of overpotential to deliver 10 mA·cm−2and displaying a small Tafel slope of 69 mV·dec−1, rivaling commercial 20% Pt/C. Stability tests reveal negligible activity loss over 48 h, underscoring the robustness of the heterostructure. The RuP2/Mn2P2O7heterojunction demonstrates markedly reduced overpotentials for the electrochemical HER process, highlighting its enhanced catalytic efficiency and improved cost-effectiveness compared to the conventional catalytic systems. This work establishes a strategy for designing a transition metal phosphide heterostructure through interfacial electronic modulation, offering broad implications for energy conversion technologies.
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