Nanoscale Research Letters
·
2022
·Vol. 17
·期 1
·1-8
·
IF 5.418
Yuan Maohui,Yang Zining,Yang Xu,Wang Linxuan,Wang Rui,Lan Sheng,Han Kai,Wang Hongyan,Xu Xiaojun
Controlling the upconversion luminescence (UCL) intensity ratio, especially pumped at 808 nm, is of fundamental importance in biological applications due to the water molecules exhibiting low absorption at this excitation wavelength. In this work, a series of β-NaYbF 4 :Er microrods were synthesized by a simple one-pot hydrothermal method and their intense green (545 nm) and red (650 nm) UCL were experimentally investigated based on the single-particle level under the excitation of 808 nm continuous-wave (CW) laser. Interestingly, the competition between the green and red UCL can be observed in highly Yb 3+ -doped microcrystals as the excitation intensity gradually increases, which leads to the UCL color changing from green to orange. However, the microcrystals doped with low Yb 3+ concentration keep green color which is independent of the excitation power. Further investigations demonstrate that the cross-relaxation (CR) processes between Yb 3+ and Er 3+ ions result in the UCL competition. Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2022
·
IF 5.999
Mei-Yan Bai,Qi Zhou,Jie Zhang,Ting Li,Jun Cheng,Qun Liu,Wen-Rong Xu,Yu-Cang Zhang
Chitosan (CS) is becoming increasingly popular in food packaging due to its natural degradability and great film-forming properties. Nevertheless, its poor antibacterial properties and inadequate antioxidant properties prevent it from being used effectively. In this study, β-cyclodextrin-epichlorohydrin (β-CD-EP) oligomers were prepared and encapsulated with natural essential oils cinnamaldehyde and thymol , and then the inclusion complexes (IC) were incorporated into chitosan in various contents to afford a series of CS-IC composite films . The impacts of IC on the morphological, mechanical, thermal, and water resistance properties, antioxidant and antibacterial activities of chitosan films, as well as the loading and sustained release behavior of IC, were thoroughly examined. The results turned out that the essential oils were well-loaded with high encapsulation efficiency and showed a significant slow-release effect. It was also found that the tensile strength and the elongation at break decreased with increasing IC contents, while the thermal stability was enhanced. The incorporation of IC dramatically promoted the antioxidant and antibacterial properties of the chitosan films towards Gram-positive bacteria. Based on our findings, chitosan films containing essential oils-loaded β-CD-EP oligomers may serve as an effective food packaging material.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Hui Dong,Le Zhao,Xu Zhu,Xiuhua Wei,Menghui Zhu,Qingmin Ji,Xueke Luo,Yintang Zhang,Yanli Zhou,Maotian Xu
Rapid, simple, accurate and highly sensitive detection of enzymes is essential for early screening and clinical diagnosis of many diseases. In this study, we report the fabrication of a turn-on ratiometric electrochemical sensor for the in situ determination of β-Galactosidase (β-Gal) based on surface engineering and the design of a molecular probe (Pygal) specific for β-Gal recognition. First, Pygal probe was synthesized and characterized, and then co-assembled with the methylene blue (MB) internal reference probe on the surface of single-wall carbon nanotubes (SWCNT)-modified carbon fiber microelectrode (CFME). The resulting CFME/SWCNT/MB + Pygal sensor is activated in the presence of β-Gal giving one peak at 0.33 V originating from the oxidation of the product of Pygal enzymatic hydrolysis (PyOH). Another oxidation peak attributed to MB appears simultaneously at −0.28 V allowing the construction of a ratiometric electrochemical sensor for β-Gal detection with improved sensitivity and accuracy. The sensor showed a linear response to β-Gal in a wide concentration range from 1.5 to 30 U L −1 and a low detection limit of 0.1 U L −1 . Moreover, the sensor demonstrated excellent selectivity against several biologically relevant hydrolases and redox-active molecules. Finally, the combination of excellent electrochemical performance and favorable physicochemical properties of CFME allowed the determination of β-Gal in the whole blood of Parkinson's Disease (PD) model mice. The workflow reported in this study provides a strategy for the design and development of sensors for the in vivo monitoring of other enzymes important for the early diagnosis of different health issues.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2022
·
IF 4.132
Amir E. Kaziem,Liupeng Yang,Yigang Lin,Hanhong Xu,Zhixiang Zhang
In this work, an enzyme-responsive nanovehicle for improving captan (CAP) contact fungicide bioactivity and translocation in plant tissues was synthesized (CAP-MSNs-β-glucan) by attaching β-glucan to the outer surface of mesoporous silica nanoparticles. CAP-MSNs-β-glucan properties were tested by FTIR, ζ-potential, DLS, XRD, TGA, FE-SEM, and HR-TEM. Cargo protection ability of CAP-MSNs-β-glucan from photolysis and hydrolysis was examined in comparison to CAP commercial formulation (CAP-CF). CAP-MSNs-β-glucan distribution in plant tissues, bioactivity against Fusarium graminearum, and biotoxicity toward zebrafish (Danio rerio) were tested and compared with that of CAP-CF. CAP-MSNs-β-glucan results showed good loading efficacy reaching 18.39% and enzymatic-release dependency up to 83.8% of the total cargo after 20 days of β-glucan unsealing. CAP-MSNs-β-glucan showed significant release protection under pH changes. MSNs-β-glucan showed excellent CAP protection from UV. CAP-MSNs-β-glucan showed better distribution in corn tissues and 1.28 more inhibiting potency to Fusarium graminearum than CAP-CF. CAP-MSNs-β-glucan showed 1.88 times lower toxicity than CAP-CF to zebrafish after 96 h of treatment. We recommend using such formulations to overcome shortcomings of contact fungicides and achieve better and sustainable farming.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER ENVIRONMENT RESEARCH
·
2022
·Vol. 94
·期 4
·e10704
·
IF 3.306
Ke-meng Zhao,Shu-shu Zhong,Jun Zhang,Cun-sheng Zhang,Zhi Dang,Ze-hua Liu
Arylsulfatase and β-glucuronidase are two important enzymes in wastewater and surface water, which play important roles on cleavage of sulfate/glucuronide estrogens. In this work, a high-performance liquid chromatography (HPLC)-based new method was firstly established for arylsulfatase/β-glucuronidase with determination of p-nitrophenyl sulfate ( p NPS)/p-nitrophenyl-β-D-glucuronide ( p NPG). The limits of detections (LODs) of the developed method for pNPS and pNPG were 0.164 and 0.098 μM, respectively. Intraday and interday reproducibility expressed as relative standard deviation (RSD) values of retention times and peak areas was 0.39%–3.68% and 0.23%–4.74%, respectively. The respective recovery efficiencies of this HPLC-based method spiking at three different concentrations for p-nitrophenol ( p NP), p NPS, and p NPG in activated sludge were 76.5%–88.1%, 79.2%–93.1%, and 84.2%–96.1%, with RSD below 3.9%. The HPLC-based method was finally applied to estimate the enzyme activity of arylsulfatase/β-glucuronidase in one activated sludge system and along which the classical spectrophotometric method was also evaluated. Compared with the classic spectrophotometric analytical method, the HPLC-based new method could simultaneously measure arylsulfatase/β-glucuronidase one time, which was convenient and time-saving. Moreover, the developed method could effectively avoid possible underestimation that the spectrophotometric method might encounter. Practitioner points A new HPLC-based method for activity estimation of arylsulfatase and β-glucuronidase was developed. The HPLC-based method can simultaneously estimate enzyme activity of both arylsulfatase and β-glucuronidase. The HPLC-based method can avoid possible underestimation that spectrophotometric method may encounter.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
·
2022
·
IF 3.712
Fan Zhang,Jingxing Li,Chengxiong Gu,Haibo Zhang
Background The role and function of microRNA (miRNA, miR)-140-5p in the calcification of vascular smooth muscle cells (VSMCs) have been explored in this study.Methods The calcium nodules formed in transfected and β-glycerophosphate (β-GP)-treated VSMCs were observed using Alizarin Red S staining, and alkaline phosphatase (ALP) activity was determined. VSMC apoptosis was detected with flow cytometry assay. The target gene of miR-140-5p was predicted and confirmed with dual-luciferase reporter assay. Relative expressions of miR-140-5p, toll like receptor 4 (TLR4) and vascular calcification-related proteins (α-smooth muscle actin, α-SMA; Msh Homeobox 2, MSX2; bone morphogenetic protein 2, BMP2; Kruppel-like factor 4, KLF4; Runt-related transcription factor 2, RUNX2) were measured through quantitative real time polymerase chain reaction (qRT-PCR) and western blot.Results MiR-140-5p upregulation reversed the effects of β-GP on downregulating miR-140-5p and α-SMA expressions, enhancing ALP activity, calcium nodule formation and cell apoptosis, and upregulating levels of MSX2, BMP2, KLF4 and RUNX2. TLR4 was the target of miR-140-5p, and offset the effects of miR-140-5p on β-GP-induced VSMCs.Conclusions MiR-140-5p upregulation represses β-GP-induced calcification of VSMCs via targeting TLR4, providing a potential therapeutic method for vascular calcification.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AICHE JOURNAL
·
2022
·Vol. 68
·期 7
·e17683
·
IF 4.167
Hui Guo,Yuchao Li,Cuncun Zuo,Yanxia Zheng,Xinpeng Guo,Jinguo Hao,Haofei Huang,Ming Wang,Tingting Ge
Homogeneous hydrogen transfer reactions of methacrolein (MAL) and isopropanol to methallyl alcohol (MAA) were investigated in batch reactor (Conv. 89%, Select 93.1%) and tubular reactor (Conv. 88.1%, Select 95%) using aluminum isopropoxide (Al(OPr i ) 3 ) as catalyst. Kinetic experiments on hydrogen transfer reactions and reaction order were investigated in batch reactor and tubular reactor. Response surface methodology was applied to optimize the optimum reaction conditions of hydrogen transfer reaction. Purification process of MAA from product mixture after hydrogen transfer reaction was simulated with Aspen Plus software; theoretical stages, reflux ratio, and feed stage of distillation tower were optimized. Density functional theory was used to investigate viable reaction pathway and to probe the catalytic mechanism between reactants and catalyst, including dehydrogenation, coupling, and hydrogenation reaction. Microscopic mechanisms of hydrogen transfer reaction from MAA to MAL were acquired in detail and could be easily extended to other series of hydrogen transfer reaction.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2022
·
IF 3.841
Jingya Liu,Sidian Zhang,Xiuying Zhao,Yi Lu,Meng Song,Sizhu Wu
Rutin is a natural flavonoid compound with various biological activities, but its low water solubility limits its applications in the food and pharmaceutical fields. Complexation with cyclodextrins (CDs) is a powerful method to improve the poorly solubility of rutin, whereas the mechanism for the inclusion still needs to be explored. This study investigated the characteristic and mechanism for inclusion of rutin with β -cyclodextrin ( β -CD) and 2,6 dimethyl- β -cyclodextrin (DM- β -CD). The molecular simulation provided the possible inclusion structure and revealed that the combination of rutin and CDs is a thermodynamically good process. It also indicated that the complex formed with DM- β -CD had better stability and water solubility compared with β -CD. Then the complexes of rutin with β -CD and DM- β -CD were prepared and characterized by FT-IR, XRD, and DSC analysis. Phase solubility studies demonstrated that the solubility of rutin was significantly enhanced by forming 1:1 stoichiometric inclusion complexes. The effect of DM- β -CD in improving the water solubility of rutin is 146% higher than that of β -CD, which was in accordance with the simulation predictions.
✓ 已验证
产品关联
DOI
高影响因子
CATALYSIS TODAY
·
2022
·
IF 6.562
Yunfei Ma,Wenhui Yue,Zehong Xu,Ziwei Ye,Jinlong Zhang
Composite nanoparticles consisting upconversion nanoparticles (β-NaYF 4 :Yb/Er) and nitrogen doped TiO 2 nanoparticles were constructed via a chemical assembly method. Efficient energy transfer was enabled since the upconverted energy matched up with the band gap of N-TiO 2 . This enables the resulting composite nanoparticles to possess excellent Rhodamine B (RhB) degradation activity under near infrared (NIR) light irradiation.
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 10
·4806-4813
·
IF 3.925
Jie Song,Leilei Sun,Hongchao Geng,Wenlong Tan,Deshuai Zhen,Qingyun Cai
β-Phase NaYF4:Yb,Tm,Gd up-conversion nanorods (UCNRs) with high uniformity were synthesized by a solvothermal method. The optimal fluorescence intensity of UCNRs was explored. After further modification with Materials of Institute Lavoisier-100(Fe) (MIL-100(Fe)) by the layer-by-layer self-assembly method, near-infrared light-triggered UCNR@MIL-100(Fe) nanomaterials with excellent antibacterial performances were constructed. A core–shell structure was constructed and confirmed by transmission electron microscopy (TEM). Reactive oxidizing species (ROS) detection indicated that the nanomaterials owned good photodynamic efficacy. The antibacterial experiments showed that the nanomaterials with 980 nm light irradiation and hydrogen peroxide (H2O2) had good antibacterial activity against Gram-negative and Gram-positive bacteria. Under 980 nm laser excitation, UCNRs generated ultraviolet and visible light to activate MIL-100(Fe) and induced a photo-Fenton reaction, which further produced oxygen species for antibacterial applications.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2022
·Vol. 33
·期 7
·3965-3981
·
IF 2.779
Ahmed Arsalan,Jia Yunming,Deb Hridam,Arain Muhammad Fahad,Memon Hafeezullah,Pasha Khalid,Huang Yi,Fan Qinguo,Shao Jianzhong
The use of piezoelectric materials has been increased due to the growing demand for wearable devices. Herein, we report the development of new copolymer poly (vinylidene fluoride-co-trifluoroethylene) (PVDF-TrFE)-based reduced graphene oxide (rGO) and multiwalled carbon nanotubes (MCNTs) loaded electrospun (E-Spun) nanofibers. The rGO-MCNTs loaded PVDF-TrFE nanofibrous mat led to the fabrication of ultra-sensitive piezoelectric pressure sensors for potential wearable health monitoring applications. The doped PVDF-TrFE solution of different weight percentages of rGO-MCNTs as fillers was prepared and used to fabricate an e-spun nanofibrous mat. Complete characterization of resultant materials were carried through diverse instruments which reveals the successful integration of rGO-MCNTs (3.2%) as a dopant that improved the β-phase up to 92%. The DSC analysis further exposes the high thermal stability of the PVDF-TrFE nanofibers mat due to the enhanced crystallinity with the addition of nanofillers. The newly developed sensor’s overall output (based on sensitivity) was calculated under a variable applied pressure range of 0.25 ~ 300 cN at 50 HZ. Results show that the pressure sensor response has improved from 16.125 ~ 0.430 kPa −1 , corresponding to a higher sensitivity under static and dynamic forces in the applied pressure range. These results are of a fundamental study and open new prospects for the hybrid nanofibrous mat as alternative electrode material in the piezoelectric pressure sensor.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2023
·
IF 6
Jiali Gu,Yanxuan Ma,Zimu Li,Hongrui Liu,Qionghua Zhang
Tert-butylhydroquinone (TBHQ) is a synthetic antioxidant found widely in foods such as cooking oil, fried baked goods and meat products, however, if consumed in excessive quantities, it may cause nutritional problems and chronic diseases. The binding of TBHQ to transport protein before and after β-cyclodextrin (β-CD) encapsulation was investigated by multiple spectroscopies and molecular docking technology. At 298 K, the binding constant, enthalpy, and entropy of bovine serum albumin (BSA) with TBHQ were (1.18 ± 1.50) × 10 4 M −1 , (−110.11 ± 15.29) kJ mol −1 and (−293.36 ± 50.87) J mol −1 K −1 at 298 K, respectively. By forming a stable complex with BSA through van der Waals forces and hydrogen bonds, TBHQ altered BSA's secondary structure and microenvironmental polarity. However, the binding affinity of TBHQ with BSA decreased after encapsulation by β-CD, but the binding distance increased. Because of the encapsulation of β-CD, the interference of TBHQ with the BSA conformation was also inhibited. Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscope study confirmed the formation of an inclusion complex between CD and TBHQ. According to UV spectroscopy, TBHQ and β-CD have a binding constant of (1.21 ± 0.03) × 10 3 M −1 . The formation of TBHQ–β-CD inclusion complex inhibited the binding of TBHQ and BSA, possibly because the inclusion of β-CD prevented TBHQ from entering the hydrophobic cavity of BSA, which prevented TBHQ from binding to BSA, alleviating the negative effects of TBHQ on BSA conformation. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging results showed that the antioxidant activity of TBHQ was not affected by β-CD encapsulation.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）