ACS Applied Bio Materials
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2025
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IF 4.7
Qianshen Tang,Jiawei Nie,Weijian Fang,Hao Xie,Weimin Wang,Hao Wang,Hang Ping,Bin Li,Zhengyi Fu
Mineralized collagen fibrils are the building blocks of bone, and the mineralization of collagen fibrils is generally regulated by noncollagenous proteins (NCPs). However, the functions of NCPs are difficult to investigate in vivo. Here, we use poly(acrylic acid) (PAA) with different molecular weights (5, 50, 450, and 4000 kDa) as analogs of NCPs and explore their effects on collagen mineralization in vitro. All the PAA molecules can promote the intrafibrillar mineralization of calcium carbonate (CaCO3) following these steps: the precursors infiltrate the gap zones of collagen, and transform into organized calcite nanocrystals within collagen. An increase in molecular weight significantly accelerates the mineralization rate of collagen films, approximately 0.67 μm min–1 at 4000 kDa, four times that of 5 kDa (0.16 μm min–1). However, the generation of contractile stress via intrafibrillar mineralization in tendons exhibits a contrary tendency. It reaches 24.2 MPa at 5 kDa, much higher than that of 4000 kDa (8.3 MPa), due to rapid mineralization causing severe extrafibrillar precipitation around the tendon. The controllable mineralization of collagen matrices may inspire the development of bone repair and regeneration in the future.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Haoran Tian,Qingyu Wu,Qiuju Wu,Chengyou Sun,Ruimei Zhang,Jie Wei,Haiyin Xu,Zhiming Liu,Chao Huang,Ping Wang
Nano zero-valent iron (nZVI) has been widely employed for the elimination of heavy metals from wastewater. However, the practical application of nZVI is greatly limited due to its fast aggregation and surface passivation. In this study, a carbon nanotube-supported nZVI composite (nZVI@CNTs) was synthesized and coupled with Shewanella oneidensis MR-1 to enhance the removal of Cr(VI) from aqueous media. The results demonstrated that the coupling of nZVI@CNTs and MR-1 (nZVI@CNTs/MR-1) exhibited superior performance in eliminating Cr(VI) compared to using nZVI@CNTs or MR-1 alone. Electrochemical characterization demonstrated that CNTs acting as electron shuttles improve the electron transfer from MR-1 to nZVI, thus facilitating the passivation layer dissolution. XRD analysis proved that MR-1 can effectively promote the dissolution of the Fe(III) passivation layer with the existence of CNTs. FTIR and XPS analyses confirmed that CNTs contain many functional groups (such as C − C and C = O) related to extracellular electron transfer, thus promoting the electron transfer of MR-1 to nZVI. Three-dimensional excitation-emission matrix (3D-EEM) analysis showed that the extracellular polymeric substances (EPS) generated from MR-1 participated in the Cr(VI) elimination. The mechanism analysis demonstrated the Fe(II) dissolved from the nZVI passivation layer reduces Cr(VI) to Cr(III) and then removes it by co-precipitation, and the EPS secreted by MR-1 also participates in the Cr(VI) adsorption and reduction. Generally, the coupling of nZVI@CNTs and MR-1 successfully addresses the drawbacks of nZVI and demonstrates a remarkable ability to remove Cr(VI).
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SENSORS AND ACTUATORS B-CHEMICAL
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2024
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IF 8
Huinan Chen,Jingyao Song,Yuanyuan Li,Dongmei Deng,Yuchen Song,Xiaoli Zhu,Liqiang Luo
Tumor biomarkers are a kind of important biomolecules for aiding cancer diagnosis and assessing tumor efficacy, among which alpha-fetoprotein (AFP) is a crucial tumor biomarker for the early diagnosis of hepatocellular carcinoma. In this study, a novel cascade signal amplifying strategy was designed for the ultrasensitive detection of AFP by DNAzyme cleaving-mediated hybridization chain reaction (HCR). The primary antibody was immobilized on the Au nanoparticles modified glassy carbon electrode, which subsequently captured AFP and the secondary antibody. Then, streptavidin was combined with the biotin-modified secondary antibody and Cu 2+ -specific DNAzyme. When Cu 2+ was present, the substrate strand of the DNAzyme would cleave and produce an ssDNA end. Finally, HCR was triggered to form long double-stranded DNA on the electrode, which would greatly inhibit the charge transfer at the electrode/solution interface. Therefore, the DNAzyme cleaving-mediated HCR strategy can be applied as cascade signal amplification for the ultrasensitive electrochemical determination of tumor biomarker AFP. Under optimal conditions, the immunosensor exhibits a broad linear range of 0.001−100 ng·mL −1 (R 2 = 0.990) and a low detection limit of 15.8 fg·mL −1 . The proposed sensor displays high sensitivity and selectivity for AFP detection, which can be employed as a universal sensing platform in clinical diagnostics.
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FOOD CHEMISTRY
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2025
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IF 9.8
Chenze Lu,Gongyan Wang,Xiaoxiao Zhang,Zhihui Fang,Xinyuan Wang,Fan Tang,Kang Ning,Min Xu,Jing Wang,Han Jiang,Ran Tao,Pei Xu
Intake of Concanavalin A (Con A), a hazardous lectin protein commonly discovered in legume vegetables, could cause various systemic symptoms including rash, nausea, vomiting, diarrhea, etc. Herein, we present a novel label-free and cost-effective electrochemical Con A aptasensor. [Fe(CN) 6 ] 3−/4- ions in testing buffer not only generate redox peaks during electrochemical scan, but also serve as electron acceptor/donor to enhance electron transfer. Anti-Con A aptamer is immobilized on surface of screen printed electrode to capture Con A molecules and form aptamer-protein complex, which hinders electron transfer near electrode surface and causes a decrease in peak current value. The constructed aptasensor displays a low detection limit of 17.88 ng/mL (around 171.92 pM), as well as good specificity against other legume lectins. The practicality of electrochemical sensors was evaluated using real-world samples, demonstrating their ability to rapid detect Con A in crude protein extracts from jack bean seeds. This work provides novel tools for rapid evaluation of Con A content in massive samples, which is required in food safety inspection and safety breeding of legume vegetables.
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BIOORGANIC CHEMISTRY
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2024
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IF 4.5
Fengxin Zheng,Suiqing Mai,Xiaolin Cen,Pei Zhao,Wenjie Ye,Jiale Ke,Shiqin Lin,Huazhong Hu,Zitao Guo,Shuqin Zhang,Hui Liao,Ting Wu,Yuanxin Tian,Qun Zhang,Jianxin Pang,Zean Zhao
The development of XOD/URAT1 dual target inhibitors has emerged as a promising therapeutic strategy for the management of hyperuricemia. Here, through virtual screening, we have identified digallic acid as a novel dual target inhibitor of XOD/URAT1 and subsequently evaluated its pharmacological properties, pharmacokinetics, and toxicities. Digallic acid inhibited URAT1 with an IC 50 of 5.34 ± 0.65 μM, which is less potent than benzbromarone (2.01 ± 0.36 μM) but more potent than lesinurad (10.36 ± 1.23 μM). Docking and mutation analysis indicated that residues S35, F241 and R477 of URAT1 confer a high affinity for digallic acid. Digallic acid inhibited XOD with an IC 50 of 1.04 ± 0.23 μM. Its metabolic product, gallic acid, inhibited XOD with an IC 50 of 0.91 ± 0.14 μM. Enzyme kinetic studies indicated that both digallic acid and gallic acid act as mixed-type XOD inhibitors. It shares the same binding mode as digallic acid, and residues E802, R880, F914, T1010, N768 and F1009 contribute to their high affinity. The anion group (carboxyl) of digallic acid contribute significantly to its inhibition activity on both XOD and URAT1 as indicated by docking analysis. Remarkably, at a dosage of 10 mg/kg in vivo, digallic acid exhibited a stronger urate-lowering and uricosuric effect compared to the positive drug benzbromarone and lesinurad. Pharmacokinetic study indicated that digallic acid can be hydrolyzed into gallic acid in vivo and has a t 1/2 of 0.77 ± 0.10 h. Further toxicity evaluation indicated that digallic acid exhibited no obvious renal toxicity, as reflected by CCK-8, biochemical analysis (CR and BUN) and HE examination. The findings of our study can provide valuable insights for the development of XOD/URAT1 dual target inhibitors, and digallic acid deserves further investigation as a potential anti-hyperuricemic drug.
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Heliyon
·
2024
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IF 3.4
Yimeng Ren,Longzhu Zhou,Yujie Shi,Yanan Yu,Weihai Xing,Qingyu Zhao,Junmin Zhang,Yueyu Bai,Jing Li,Chaohua Tang
Beef flavor profiles are strongly influenced by cooking methods and lipid composition. However, the effect of instant-boiling on the aroma of different beef slices was unclear. This study investigated the lipid profiles and instant-boiling volatile profiles of chuck tender (M. Supraspinatus ), sirloin (M. Longissimus dorsi ) and silverside (M. Biceps femoris ). Quantitative lipidomics identified 336 lipid molecular species, of which 84–112 were quantitatively different among the three beef slices. Sirloin had lower phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol and free fatty acids than chuck tender and silverside. The unsaturated fatty acid acyl chains in phosphatidylethanolamine differed significantly. Solid phase microextraction-gas chromatography-olfactometry-mass spectrometry (SPME-GC-O-MS) identified hexanal, octanal, nonanal, decanal, (E)-2-octenal, (E)-2-nonenal, (E)-2-undecenal, (E,E)-2,4-nonadienal, (E,E)-2,4-decadienal, 1-octen-3-ol, 2-pentylfuran and acetoin as the aroma-active compounds of instant-boiled beef. Unsaturated free fatty acids and phosphatidylglycerols with unsaturated fatty acid residues positively correlated with the aroma-active compounds and might be crucial in flavor differences among the three beef slices. These findings provide greater understanding of the lipid and instant-boiling aroma-active compound profiles in chuck tender, sirloin and silverside, and reflect the suitability of different beef slices for instant-boiling from the aroma perspective.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2024
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IF 5.3
Ping Wang,Boyuan Liu,Qingqing Wang,Yue Wang,Xiuqian Gao,Jingxin Gou,Haibing He,Yu Zhang,Tian Yin,Xiangqun Jin,Xing Tang
Safe and effective Cu 2+ supplementation in local lesion is crucial for minimizing toxicity of DSF-based chemotherapy. Targeted delivery of Cu 2+ appears more promising. Intraperitoneal chemotherapy for peritoneal carcinoma (PC) establishes “face-to-face” contact between targeted nanocarriers and tumor tissue. Herein, this study developed a biodegradable, injectable thermosensitive hydrogel that coencapsulating DSF submicroemulsion (DSF-SE) and folate-modified liposome loading glycyrrhizic acid-Cu (FCDL). FCDL acted as ‘beneficial horse’ to target the tumor-localized folate receptor, thus liberating Cu 2+ in tumor nidus. The prepared FCDL and DSF-SE were found with uniform sizes (160.2 nm, 175.4 nm), low surface charge (−25.77 mV, −16.40 mV) and high encapsulation efficiency (97.93 %, 90.08 %). In vitro drug release profile of FCDL, DSF-SE and FCDL＆DSF-SE@G followed a sustained release pattern. And the release behavior of Cu 2+ from FCDL was pH-related, i.e., Cu 2+ was released faster under acidic condition. When FCDL and DSF-SE were loaded into an PLGA-PEG-PLGA-based hydrogel system, FCDL＆DSF-SE@G was formed to ensure separated delivery of Cu 2+ and DSF in space but synchronized release over time. The rheology experiment showed a satisfactory gelling temperature of 32.7 °C. In vitro cytotoxicity study demonstrated that FCDL＆DSF-SE@G significantly lowered the IC 50 of free Cu 2+ /DSF, Cu 2+ /DSF hydrogel and non-targeted analogue by almost 70 %, 65 % and 32 %, respectively. Accordingly, in tumor-bearing mice, FCDL＆DSF-SE@G augmented the tumor inhibition rates for the same formulations by 352 %, 145 % and 44 %, respectively. The main mechanism was attributed to higher uptake of FCDL and DSF-SE, resulting in increased Cu(DDTC) 2 formation, ROS production and cell apoptosis. In conclusion, this targeted nanotherapy approach with dual-nanocarriers loaded hydrogel system, with its focus on face-to-face contact between nanocarriers and tumor tissues in the peritoneal cavity, holds significant promise for intraperitoneal chemotherapy in PC.
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ADVANCED FUNCTIONAL MATERIALS
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2025
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IF 19
Huakang Yang,Zining Zhang,Yudong Guo,Shuwen Yuan,Xiao Liu,Dongxiang Luo,Siyu Ye
Solar-driven photocatalytic CO 2 reduction coupled with the oxidation of organic compounds to value-added chemicals is a promising strategy, which can utilize both photogenerated electrons and holes. Here, a gradient alloyed CdS@Zn x Cd 1-x S@ZnS quantum dots (quasi-Type II band structure) capped with Cl − ligands demonstrate great potential in the simultaneous conversion of CO 2 and 1-phenylethanol into syngas and pinacol. The passivation with Cl − maximizes the exposure of surface-active sites and improves their electronic structure, providing a prerequisite for the efficient execution of redox reactions. More importantly, the p–π conjugation between chloride-ion ligands and the benzene ring provides a bridge for the ultrafast transfer of holes, which greatly promotes the activation of the Cα─H bond in 1-phenylethanol to release a mass of reactive free hydrogen. This process effectively lowers the thermodynamic energy barrier for CO 2 reduction, even as the excessively high kinetic energy barriers arising from the accumulation of excess protons also enable them to self-couple, leading to the generation of syngas (CO ≈ 75 mmol g −1 h −1 ; selectivity ≈ 69.86%). This work provides insights for future artificial photosynthesis to achieve high activity and cost-effective conversion of CO 2 into storable renewable resources and the co-production of value-added substances through synergistic organic oxidation.
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ADVANCED MATERIALS
·
2024
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IF 27.4
Chengjin Ding,Jiao Min,Yongkang Tan,Liuting Zheng,Ruxuan Ma,Ruyi Zhao,Huiyue Zhao,Qingqing Ding,Hongshan Chen,Da Huo
Senescence plays a critical role in the development and progression of various diseases. This study introduces an amorphous, high-entropy alloy nanozyme-based therapeutic designed to combat senescence. We start by screening ligands to optimize the synthesis of the amorphous nanozyme. By adjusting the nanozyme's composition and surface properties, we analyze its catalytic performance under both normal and aging conditions, confirming that peroxide and superoxide dismutase activity are crucial for its anti-aging therapeutic function. Subsequently, we validate the chiral-dependent therapeutic effects and demonstrate the senolytic performance of D-handed PtPd2CuFe across several aging models. Through proteomic and transcriptome analyses, we explore the mechanism underlying the senolytic action exerted by nanozyme in depth. We confirm that exposure to senescent conditions led to the enrichment of copper and iron atoms in the nanozyme's lower oxidation states, disrupting the iron-thiol cluster in mitochondria and lipoic acid transferase, as well as oxidizing unsaturated fatty acids. These actions triggered a cascade of cuproptotic and ferroptotic cell death. Additionally, we found that the nanozyme's anti-aging effects depend on concentration. Even an ultra-low dose of the therapeutic can act as a senomorphic, reducing the effects of senescence. Given its broad-spectrum action and concentration-adjustable anti-aging potential, we confirmed the remarkable therapeutic capability of D-handed PtPd2CuFe in managing atherosclerosis, a disease involving various types of senescent cells. This article is protected by copyright. All rights reserved
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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2017
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IF 2.466
Huanan Wang,Wenqing Xu
Due to higher transmembrane potential of tumor cells, enhanced accumulation of cationic drugs in tumor mitochondria has been attributed to a higher (more negative inside) mitochondrial transmembrane potential compared with normal cells, emerging researchers are focus on developing mitochondria-targeted antitumor drugs. Coumarins showed great potential on antitumor, but mitochondria-targeted coumarin derivatives have not been reported. In the present study, we synthesized mitochondria-targeted-methyl coumarin (mito-methyl coumarin) through coupling 6-methyl coumarin to TPP. We confirmed that mito-methyl coumarin inhibited HeLa cells proliferation selectively, induced ROS generation, reduced mitochondrial membrane potential , promoted mitochondria Ca 2+ accumulation, decreased mitochondria mass and induced HeLa cells apoptosis, but methyl coumarin did not. These results demonstrate that we succeed in synthesizing a novel mitochondria-targeted drug, mito-methyl coumarin, which is effective in inhibiting HeLa cells proliferation and inducing HeLa cells apoptosis through promoting ROS generation and mitochondria Ca 2+ accumulation.
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ANALYTICAL CHEMISTRY
·
2024
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IF 6.7
Miao Zhang,Haowei Zhang,Yunlei Zhou,Huanshun Yin,Zhengkun Yu,Xinyue Zhang,Shiyun Ai,Minghui Wang
The OPECT biosensing platform, which connects optoelectronics and biological systems, offers significant amplification and more possibilities for research in biological applications. In this work, a homogeneous organic photoelectrochemical transistor (OPECT) biosensor based on a Bi2S3/Bi2MoO6 heterojunction was constructed to detect METTL3/METTL14 protein activity. The METTL3/METTL14 complex enzyme was used to catalyze adenine (A) on an RNA strand to m6A, protecting m6A-RNA from being cleaved by an E. coli toxin (MazF). Alkaline phosphatase (ALP) catalyzed the conversion of Na3SPO3 to H2S through an enzymatic reaction. Due to the adoption of the strategy of no fixation on the electrode, the generated H2S was easy to diffuse to the surface of the ITO electrode. The Bi2S3/Bi2MoO6 heterojunction was formed in situ through a chemical replacement reaction with Bi2MoO6, improving photoelectric conversion efficiency and realizing signal amplification. Based on this “signal on” mode, METTL3/METTL14 exhibited a wide linear range (0.00001–25 ng/μL) between protein concentration and photocurrent intensity with a limit of detection (LOD) of 7.8 fg/μL under optimal experimental conditions. The applicability of the developed method was evaluated by investigating the effect of four plasticizers on the activity of the METTL3/METTL14 protein, and the molecular modeling technique was employed to investigate the interaction between plasticizers and the protein.
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Heliyon
·
2024
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IF 3.4
Haoruo Jia,Ziyuan Zheng,Jining Qu,Hongtao Yu,Zhoujun Zhu,Qingda Lu,Fei Su,Yating Yang,Tongtong Feng,Qiang Jie
Although Zeolitic Imidazolate Framework-8 (ZIF-8) shows significant promise in chemodynamic therapy of bacterial infections due to its large specific surface area and enzyme-like activity, it still faces a considerable gap compared to natural enzymes. The dependency on low pH and high concentrations of hydrogen peroxide ((H 2 O 2 ) is a major factor limiting the clinical progress of nanozymes. Single-atom nanozymes (SA-zyme), which exhibit superior catalytic performance, are expected to overcome this limitation. In this study, we used ZIF-8 as a template to prepare structurally regular molybdenum-based single-atom nanozymes (Mo-zyme) by coordinating molybdenum atoms with nitrogen atoms within the zeolitic imidazolate framework and evaporating the zinc element at high temperatures. The cascade catalytic performance of the nanodrugs was enhanced by loading glucose oxidase (GOx) and encapsulating it with a hyaluronic acid (HA) layer to form a composite (Mo/GOx@HA). Upon contact with hyaluronidase from bacteria in infected tissues, the cascade reaction is triggered, resulting in the degradation of the HA shell, and releasing the encapsulated GOx. Once exposed, GOx catalyzes the oxidation of glucose into gluconic acid, resulting in a localized decrease in pH and continuous production of H 2 O 2 . The combination of lowered pH and increased H 2 O 2 concentration significantly amplifies the catalytic activity of the Mo-zyme. This enhanced activity facilitates the in situ generation of hydroxyl radicals (•OH) on the bacterial surface, leading to effective and efficient bacterial eradication. Wound infection treatment has demonstrated that the as-prepared Mo/GOx@HA exhibits excellent antibacterial and anti-inflammatory activity. This work provided a promising enzymatic cascade reaction nanoplatform for the treatment of bacteria infected wounds.
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危险品化学品经营许可证（带存储）