JACS Au
·
2024
·
IF 8.5
Yaoyun Wu,Yaozhong Cui,Wei Song,Wanqing Wei,Zhizhen He,Jinyang Tao,Dejing Yin,Xiulai Chen,Cong Gao,Jia Liu,Liming Liu,Jing Wu
l-Amino acid oxidase (LAAO) is an important biocatalyst used for synthesizing α-keto acids. LAAO from Rhodococcus opacus (RoLAAO) has a broad substrate spectrum; however, its low total turnover number limits its industrial use. To overcome this, we aimed to employ crystal structure-guided density functional theory calculations and molecular dynamic simulations to investigate the catalytic mechanism. Two key steps were identified: S → [TS1] in step 1 and Int1 → [TS2] in step 2. We reprogrammed the transition states [TS1] and [TS2] to reduce the identified energy barrier and obtain a RoLAAO variant capable of catalyzing 19 kinds of l-amino acids to the corresponding high-value α-keto acids with a high total turnover number, yield (≥95.1 g/L), conversion rate (≥95%), and space-time yields ≥142.7 g/L/d in 12–24 h, in a 5 L reactor. Our results indicated the promising potential of the developed RoLAAO variant for use in the industrial production of α-keto acids while providing a potential catalytic-mechanism-guided protein design strategy to achieve the desired physical and catalytic properties of enzymes.
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Journal of Functional Foods
·
2024
·
IF 3.8
Yunfei Wei,Meiyu Jin,Hao Yu,Xiaoning Hou,Lilei Zhao,Lu Ding,Jiaqi Cheng,Jiazhang Qiu,Haihua Feng
Glycyrrhiza glabra L. is a common heritage herbal medicine rich in antioxidant compounds such as flavonoids and triterpenoids. Non-alcoholic fatty liver disease has been the global prevalent chronic liver disorder. To investigate the curative role and mechanism of the Glycyrrhiza glabra L. crude extract on NAFLD, in vitro , we used oleic acid, palmitic acid, ML-385 and 3-MA to stimulate HepG2 cells. In vivo , we used Tyloxapol to inject mice intraperitoneally to establish an acute model, and high-fat diet to feed wild-type, Nrf2 -/- and ATG5 flox/flox mice to establish a chronic model. We used high performance liquid chromatography to analyse the content of major bioactive components in the Glycyrrhiza glabra L. crude extract. Our study found the Glycyrrhiza glabra L. crude extract could effectively alleviate lipid accumulation in non-alcoholic fatty liver disease, activate Nrf2 and promote autophagy. However, this alleviating effect disappeared when the Nrf2 and autophagy were inhibited separately. Our study demonstrates that crude extraction of active ingredients from traditional herbal medicines may be a potentially inexpensive, rapid and safe approach to alleviate lipid accumulation in non-alcoholic fatty liver disease.
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Food & Function
·
2020
·Vol. 11
·期 11
·10149-10160
·
IF 4.171
Yao Ren,Hui Wu,Yuanlong Chi,Ruijie Deng,Qiang He
Tilapia (Oreochromis nilotica L.) skin collagen is a meritorious commercial resource to be exploited. The purpose of this study was to obtain, evaluate, and characterize tilapia skin collagen-derived antioxidant hydrolysates (TSCP). AAPH-induced erythrocyte hemolysis assay and antifatigue test in mice were implemented. It was indicated that TSCP treatment at 1 mg mL−1 could effectively attenuate AAPH-induced erythrocyte hemolysis rate from 56.35 ± 2.46% to 18.78 ± 2.48% (p < 0.01). A 2.5 mg/(10 g d) dose of TSCP intragastric administration could observably prolong the exhaustive swimming time of the loaded mice and its mechanism was multiple, including the decrease in the levels of serum lactic acid, serum urea nitrogen, and creatine kinase activity, thus improving the contents of liver and muscle glycogen and endogenous SOD activity. Five oligopeptides from the antioxidant fraction were identified as Gly-Hyp, Glu-Asp, Asp-Hyp-Gly, Glu-Pro-Pro-Phe, and Lys-Pro-Phe-Gly-Ser-Gly-Ala-Thr and then synthesized. Among them, the octapeptide exhibited the strongest antioxidant capacity. Therefore, tilapia skin-derived collagen is a meritorious edible resource for producing commercial functional foods, thus helping to scavenge radicals, protecting erythrocytes, and further resisting fatigue.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
·
IF 5.162
Hong Yao,Lei Wang,Xin Tang,Zhuo Yang,Huanyong Li,Chongzhen Sun,Xiyang Wu,Dan Xu
In this research, two novel polysaccharides (S1 and S2) from Solanum nigrum L were extracted and purified. Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined. Then, the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production were investigated using an in vitro fermentation model. Results showed that S1 and S2 have different impacts on human gut microbiota in vitro . S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid. Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably. We concluded that S1 and S2 may have potential prebiotic functions.
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POLYMER BULLETIN
·
2025
·Vol. 83
·期 1
·31
·
IF 4
Huang Ruoxi,Yi Yaxin,Zhang Shiyu,Wang Jiaxin,Yuan Haikuan,Lu Jie
A stimulus-responsive drug delivery system holds promise for reducing adverse effects and enhancing the bioavailability of chemotherapeutic agents. In this study, amino-terminated dextran was utilized as a macromolecular initiator to prepare a range of amphiphilic dextran-b-poly(ε-benzyloxycarbonyl- l -lysine) (Dex-b-PZLL) copolymers with varying hydrophobic segment lengths via ring-opening polymerization (ROP). Folic acid (FA) was further grafted onto the dextran side chains via esterification (FA-Dex-b-PZLL), offering the potential tumor-specific recognition. The resulting copolymers were able to self-assemble into stable spherical micelles via dialysis and efficiently encapsulated the hydrophobic drug letrozole (LTZ). A systematic assessment was conducted on the drug encapsulation efficiency/loading capacity, drug release performance in vitro, and biocompatibility of the copolymers under aqueous conditions, indicating that these properties of the FA-Dex-b-PZLL micelles mainly depended on the copolymer composition. The optimized micelles, with a balanced hydrophilic/hydrophobic ratio, achieved a drug loading capacity with approximately 8.49% and an encapsulation efficiency with about 46.39%. Moreover, LTZ/FA-Dex-b-PZLL micelles exhibited pH-responsive release, with cumulative release rates within 72 h of 20.74%, 27.14% and 62.96% in phosphate buffer at pH 7.4, 6.5 and 4.5, respectively, indicating the accelerated release under acidic conditions. In addition, the micelles showed good cytocompatibility according to the CCK-8 assay. Graphical abstract
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CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Keguang Mu,Dalun Zhang,Ziqiang Shao,Dujian Qin,Yalong Wang,Shuo Wang
l -Dopa functionalized halloysite nanotubes (HNTs) were prepared by the self-polymerization of l -dopa in the weak alkaline condition. Then different contents of l -dopa coated HNTs (LPDHNTs) were blended into cellulose acetate to prepare enhanced performance ultrafiltration membranes via the phase inversion method . The HNTs and LPDHNTs were characterized by FTIR, XPS, and TEM anysis. And the membranes morphologies, separation performance, antifouling performance, mechanical properties and hydrophilicity were also investigated. It was found that the composite membranes exhibited excellent antifouling performance. The pure water flux of 3.0 wt% LPDHNTs/CA membrane increased from 11.4 L m −2 h −1 to 92.9 L m −2 h −1 , while the EA rejection ratio of the membrane was about 91.2%. In addition, the mechanical properties of the resultant membranes were strengthened compared with the CA ultrafiltration membrane.
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NEW JOURNAL OF CHEMISTRY
·
2014
·Vol. 38
·期 12
·6223-6229
·
IF 3.159
Wanyun Liu,Junchao Wei,Yiwang Chen
Multifunctional electrospun composite nanofibrous scaffolds have attracted much interest as drug delivery vehicles and in bioimaging application for real-time tracing the whole process of postoperative therapy. Novel poly(L-lactide) (PLLA) composite nanofibers loaded with water-soluble fullerene C70 nanoparticles and paclitaxel were successfully fabricated. The nanofibers with the average diameter of fibers ranging from 350 to 750 nm were uniform and their surfaces were reasonably smooth. The nanofibers showed an excellent hydrophilic surface and good mechanical properties. The in vitro release results demonstrated that the release rate of paclitaxel could be controlled by the content of C70 nanoparticles. With the increase of the content of C70 nanoparticles, the drug release rate became faster with increased total release amount. The composite nanofibers used as substrates for cytotoxicity and bioimaging in vitro were evaluated with human liver carcinoma HepG-2 cells. Paclitaxel was released from the composite nanofibers without losing cytotoxicity, the drug-loaded composite nanofibers inhibited the proliferation of HepG-2 cells effectively. Meanwhile, the fluorescent signal of C70 nanoparticles could be detected in HepG-2 cells, which reflected the growth state of cells clearly. These results strongly suggested that these PLLA composite nanofibers could be used in the fields of tissue engineering, drug delivery and bioimaging.
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Journal of Macromolecular Science Part B-Physics
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2017
·
IF 0.828
Qiao Zhang,Zhihua Zhou,Cheng Peng,Tianlong Huang,Wenjuan Liu,Qingquan Liu,Hu Zhou,Wei Wang,Hua Yan
Three-dimensional biodegradable porous scaffolds play an important role in tissue engineering. A new polymer based on maleated poly(lactic-co-glycolic acid) (MPLGA) was synthesized using direct melt copolymerization from maleic anhydride (MAH), D, L-lactide, and glycolide monomers. MPLGA porous biodegradable scaffolds were prepared by a solution-casting/salt-leaching method. The effects of content and size of the NaHCO3 porogen on the compressive strength of the MPLGA scaffolds were investigated, and the effect of content of the porogen on the porosity of the MPLGA scaffolds was also studied. The results indicated that MAH was grafted onto PLGA successfully and MPLGA scaffolds with interconnective and open pore structure were obtained. Increasing content of NaHCO3 porogen resulted in an increase of porosity and decrease of the compressive strength of the MPLGA scaffolds with the compressive strength of the scaffolds also decreasing with increasing porogen size.
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BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
·
2016
·Vol. 64
·期 2
·290-300
·
IF 1.429
Zhen-Tao Song,Ming-Jun Zhu
Fermentation of herb Polygonum hydropiper L. (PHL) and cassava pulp (CP) for feed additive production with simultaneous flavonoid dissolution was investigated, and a two-stage response surface methodology (RSM) based on Plackett−Burman factorial design (PB design) was used to optimize the flavonoid dissolution and protein content. Using the screening function of PB design, four different significant factors for the two response variables were acquired: factors A (CP) and B (PHL) for the flavonoid dissolution versus factors G (inoculum size) and H (fermentation time) for protein content. Then, two RSMs were used sequentially to improve the values of the two response variables separately. The mutual corroboration of the experimental results in the present study confirmed the validity of the associated experimental design. The validation experiment showed a flavonoid dissolution rate of 94.00%, and a protein content of 18.20%, gaining an increase in 21.20% and 199.10% over the control, respectively. The present study confirms the feasibility of feed additive production by Saccharomyces cerevisiae with CP and PHL and simultaneous optimization of flavonoid dissolution and protein content using a two-stage RSM.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Yangyang Chen,Min Zhang,Arun S. Mujumdar,Yaping Liu
Orange juice is popular with consumers because of its unique taste, flavor as well as nutritional value which accounts for a large proportion of the global juice market. Concentrated orange juice (COJ), undergoes browning during storage seriously affecting its quality and commercial. This study was the first to evaluate the effects of epigallocatechin gallate (EGCG) and l -cysteine (L-cys) on Maillard browning of COJ during storage. The results show that EGCG and L-cys reduce significantly the production of 5-hydroxymethylfurfural (5-HMF), the characteristic product of Maillard reaction (MR), and the combined use of EGCG and L-cys was even more effective. Furthermore, the color of the treated COJ displayed negligible change after storage, with higher L* and b* values and marginally lower a* values. The flavor, taste, main volatile substances as well as rheological properties of the treated COJ were also determined; none of these properties were affected adversely during storage. This treatment method ensures that the key sensory quality and material properties of COJ are preserved by the proposed treatment while inhibiting Maillard browning during storage.
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ACS Applied Bio Materials
·
2025
·
IF 4.7
Zhu Gao,Xinping Han,Wenyue Gao,Jianghua Li,Cuijuan Wang
Bacterial susceptibility testing and real-time inactivation are critical for safeguarding public health with broad applications in food safety monitoring and clinical diagnostics. For pH-dependent bacterial determination and hyperthermal inactivation applications, we developed needle-shaped ZnO/ZIF-L systems through a zeolitic imidazolate framework coating of zinc oxide by a self-template strategy. The pH-dependent ZnO/ZIF-L-embedded hydrogels were prepared by UV light-mediated olefin photopolymerization using acrylamide, N,N’-methylenebis(acrylamide), hydroxyethyl methacrylate, bromothymol blue (BTB), and ZnO/ZIF-L. The hydrogel achieves rapid visual detection of bacterial infections through a pH-responsive colorimetric transition, leveraging BTB to signal acidic microenvironmental shifts characteristic of bacterial proliferation. The ZnO/ZIF-L photosensitizers can enhance charge separation and transfer and generate highly reactive oxygen species. Escherichia coli and Staphylococcus aureus can be effectively inactivated for ZnO/ZIF-L hydrogels by a physical disruption/photodynamic synergistic antibacterial mechanism. The antibacterial efficiency can reach as high as 99%. The proposed strategy not only provides a sensitive strategy for bacterial determination but also implements efficient inactivation of bacteria simultaneously. This strategy combines real-time bacterial biosensing with on-demand antimicrobial action in a single platform, overcoming the limitations of conventional sequential detection-treatment approaches.
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JOURNAL OF CONTROLLED RELEASE
·
2025
·
IF 11.5
Jiangai Long,Sheng Ma,Renming Wan,Aolin Sun,Zhenyi Zhu,Ruoyi Wang,Zhaopei Guo,Wantong Song
Cationic polymers have garnered considerable attention as mRNA carriers due to their structural tunability and chemical diversity. Nevertheless, their broader application remains limited by poor stability under physiological conditions and cytotoxicity associated with excessive charge density. Here, we report thiourea-modified poly( l -lysine) (PLL-T) carriers constructed by combinatorial side-chain modification with ionizable and hydrophobic isothiocyanates. The resulting thiourea linkage introduced two hydrogen bonding donor sites, enabling stronger hydrogen bonding with mRNA. These hydrogen bonding could synergize with electrostatic and hydrophobic interactions, forming multiple cooperative interactions with mRNA. This design enhanced mRNA encapsulation, colloidal stability, and high transfection efficiency without increasing cytotoxicity. The optimized PLL-1A1B(T) variant achieved over 10,000-fold higher transfection efficiency than the parent polymer PLL, and outperformed the commercial gold-standard Lipofectamine MessengerMAX in both HEK293T and RAW264.7 cells. Compared with its amide-linked analogue PLL-1A1B(A), in which the amide linkage provided only a single hydrogen bonding donor site, PLL-1A1B(T) exhibited superior mRNA encapsulation (91 % vs. 42 %), higher storage stability, and over 50-fold increase in intramuscular transfection. Unlike PLL-1A1B(A), which induced high levels of inflammation, PLL-1A1B(T) exhibited minimal inflammation in vivo. Importantly, PLL-1A1B(T) enabled efficient delivery of mRNA cancer vaccines and elicited potent CD8 + T cell mediated antitumor immunity. A single administration of PLL-1A1B(T)@E6/E7 mRNA vaccine achieved 77 % tumor suppression rate (TSR) in the TC-1 tumor model. Moreover, in combination with anti PD-1, PLL-1A1B(T)@OVA mRNA vaccine achieved 90 % TSR in the B16-OVA tumor model. This study demonstrates hydrogen bonding-driven carriers engineering as a versatile strategy to tune nucleic acid-carrier interactions, offering a potent and safe platform for mRNA therapeutics.
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