Bioengineered
·
2018
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IF 1.639
Yanshun Kang,Xiaolin Xu,Huiran Pan,Jing Tian,Weihua Tang,Siqi Liu
In this report, the decolorization features of extracellular enzymes and mycelia separately prepared from Aspergillus sp. TS-A CGMCC 12,964 (120 h) were investigated. The fermentation broth of TS-A degraded 98.6% of Mordant Yellow 1 (50 mg/L) at an initial pH 6 within 1 h with over 70% of the dye (50 mg/L) degraded by extracellular enzymes and 18.8% removed by live mycelia. The degradation products of the dye were analyzed by UV-Vis and FTIR spectra. The decolorization rates of extracellular enzymes and mycelia were examined under different contact periods, dye concentrations and pH values. The extracellular enzymes exhibited excellent degradation activity under weak acidic conditions. In addition, biosorption models of mycelia fitted well the Langmuir isotherm model and the pseudo-second-order kinetic equation. Although the decolorization process was achieved through the synergistic effects of mycelia and extracellular enzymes, decolorization was dominated by the biodegradation activity of the extracellular enzymes from TS-A.
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高影响因子
Horticulturae
·
2024
·Vol. 10
·期 1
·48
·
IF 3.1
Li-Rong Zhou,Hai-Jun Hu,Jie Wang,Yong-Xing Zhu,Xue-Dong Zhu,Jia-Wei Ma,Yi-Qing Liu
To counter the harmful impacts of agricultural chemicals on the environment and human health, there is an increasing demand for safe, eco-friendly, and potent plant-based biopesticides. In this study, we aimed to investigate the antimicrobial effects of ginger essential oil and selected volatile compounds (linalool, eugenol, citral, and cinnamaldehyde [CA]) againstFusarium oxysporumFOX-1. Minimum inhibitory concentrations (MICs) were determined using the mycelium growth inhibition method. The compound CA exhibited the most potent antifungal effect againstF. oxysporumFOX-1 and was selected for further investigation. After treatment with CA at 1/2 MIC or MIC, the spore germination ofF. oxysporumFOX-1 was significantly inhibited at 12 h. Furthermore, microscopic observation revealed that CA treatment resulted in the morphological degradation ofF. oxysporumFOX-1. CA destroyed the cell membrane integrity ofF. oxysporumFOX-1, increasing the relative conductivity and the leakage of intracellular protein, nucleic acids, and malondialdehyde, affecting the integrity and metabolism of the cell membrane. The effects were positively related to CA concentration. Additionally, in vivo experiments with rhizome sections showed that CA significantly reduced the pathogenicity ofF. oxysporumFOX-1. Overall, these findings provide evidence for the potential of using ginger essential oil components as fungicides, offering a basis for future research to develop robust and eco-friendly plant-derived fungicides that serve as a sustainable means to reduce fungus-driven agricultural losses.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Zhu Liu,Shoulu Yang,Zhongwei Wang,Ning Ji,Xingong Li,Yingfeng Zuo
The effect of moisture on the volatile organic compound (VOC) adsorption on biomass-derived metal–organic framework composites is significant in practical applications. In this study, wood-based metal organic frameworks (MOF199-W) and hydrophobic MOF199-W (HMOF199-W) were developed using HKUST-1 (MOF199) and long alkyl chains to investigate the adsorption performance of toluene and enhance the selectivity of adsorption under high humidity conditions. The results demonstrated that HKUST-1 could be anchored onto the surface of wood vessels and fibers through coordination with Cu 2+ , resulting in an improved surface area in comparison to natural wood. The toluene adsorption capacity of MOF199-W with a 20 % loading was comparable to that of an equivalent amount of MOF199, with adsorption capacities of 323.5 mg/g and 369.9 mg/g, respectively. Further, the Yoon-Nelson model was found to adequately describe the dynamic adsorption process. Following the introduction of long alkyl chains, MOF199 and MOF199-W were endowed with strong hydrophobic character (water contact angle of 107.0 ◦ , 104.0 ◦ , and water uptake of 30.5 mL/g, 55.3 mL/g). With the increase in humidity, the adsorption performance of MOF199 and MOF199-W for toluene sharply decreased, but HMOF199 and HMOF199-W still maintained the adsorption capacities at 304.7 mg/g and 226.7 mg/g, even under 80 % RH conditions. Therefore, HMOF199-W has shown great potential in wooden building materials with the ability for toluene capture under humid conditions.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Ya-Lan Sun,Ya-Hui Sun,Ke Wang,Pengjun Xu,Jun-Feng Dong
Helicoverpa armigera is one of the most serious pests attacking tobacco around the world. Nicotiana rustica is widely used as a trap crop in the fields of Nicotiana tabacum in China. However, the chemical and molecular bases guiding N. rustica recognition in H. armigera are still unknown. In this study, we found 15 volatiles emitted from N. rustica induced EAG responses of H. armigera, while β-ocimene, α-pinene, and linalool were attractive to the moths in Y-tubes. Oviposition tests showed that H. armigera prefers to lay eggs on tobacco plants treated with β-ocimene and α-pinene. Further assays showed that an antenna-enriched chemosensory protein, HarmCSP1, binds robustly with β-ocimene, benzaldehyde, and (Z)-3-hexenyl acetate. Structural analyses revealed that several key amino acid residues are involved in ligand binding. This study increases the understanding of the chemosensory mechanism of H. armigera’s recognition of N. rustica and provides the foundation for the design of attractants for H. armigera management.
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Journal of Functional Biomaterials
·
2024
·Vol. 15
·期 7
·185
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IF 5
Yu Wu,Sen Xu,Fengjuan Ding,Weibing Zhang,Haiyan Liu
In this work, a new type of FE-1 COF material is prepared by a reversible imine condensation reaction with diaminoferrocene and diaminodiformaldehyde as materials. The material is connected by imine bonds to form a COF skeleton, and the presence of plenty of nitrogen-containing groups gives the material good hydrophilicity; the presence of metal Fe ions provides the material application potential in the enrichment of phosphopeptides. According to the different binding abilities of N-glycopeptide and phosphopeptide on FE-1 COF, it can simultaneously enrich N-glycopeptide and phosphopeptide through different elution conditions to realize its controllable and selective enrichment. Using the above characteristics, 18 phosphopeptides were detected from α-casein hydrolysate, 8 phosphopeptides were detected from β-casein hydrolysate and 21 glycopeptides were detected from IgG hydrolysate. Finally, the gradual elution strategy was used; 16 phosphopeptides and 19 glycopeptides were detected from the α-casein hydrolysate and IgG hydrolysate. The corresponding glycopeptides and phosphopeptides were identified from the human serum. It proves that the FE-1 COF material has a good enrichment effect on phosphopeptides and glycopeptides.
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BIOCHEMICAL PHARMACOLOGY
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2025
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IF 5.6
Lijie Gao,Jiawei Liu,Rui Zhang,Xi Chen,Mo Wang,Yujia Dong,Mykhaylo S. Frasinyuk,Wen Zhang,David Watt,Wenxiang Meng,Jun Xue,Chunming Liu,Yu Cheng,Xifu Liu
Colorectal cancer (CRC), one of the diseases posing a threat to global health, according to the latest data, is the third most common cancer globally and the second leading cause of cancer-related deaths. The development and refinement of novel structures of small molecular compounds play a crucial role in tumor treatment and overcoming drug resistance. In this study, our objective was to screen and characterize novel compounds for overcoming drug resistance via the B Lymphoma Mo-MLV insertion region 1 (Bmi-1) reporter screen assay. The stable cell line harboring the Bmi-1 reporter gene was utilized to screen 300 compounds, leading to the identification of an amino-pyrimidine compound, APD-94 . In vitro , APD-94 markedly inhibited cancer cell proliferation and decreased Bmi-1 expression at both the RNA and protein levels. In vivo , APD-94 repressed the growth of HT29 cell xenografts in NOD/SCID mice without notable side effects. Flow cytometry results demonstrated that APD-94 induced G2/M phase arrest and apoptosis in cells. APD-94 was identified as a novel inhibitor of microtubule polymerization by directly targeting the tubulin. Furthermore, APD-94 was more effective in overcoming the resistance to paclitaxel in paclitaxel-resistant A549/Tax cells. This bifunctional inhibitor is a promising candidate drug for CRC treatment.
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INORGANIC CHEMISTRY
·
2024
·
IF 4.3
Ting Yang,Zheng Chen,Ziqing Wang,Jiayuan Yu,Changjiu Xia,Hongxia Liu,Lei Liu,Xinxin Peng,Yibin Luo,Xingtian Shu
Disulfide is an important organic reagent and synthetic intermediate that is widely used in organic synthesis, polymers, and other fields, but its synthesis still suffers from many environmental pollution and economic problems. Here, we present an environmentally friendly and efficient base-free aerobic oxidative thiol coupling catalyzed by heterogeneous CoOx nanoclusters entrapped in hierarchical silicalite-1 zeolite, synthesized by combining silane pore expansion and metal coordination methods under hydrothermal conditions. It is confirmed that open hierarchical channels favor mass diffusion, and the chemical valence of Co species in CoOx/h-S-1-H is +2, which is different from that of Co3O4 particles in CoOx/h-S-1-I. CoOx nanoclusters, are strongly fixed in the channels of silicalite-1 zeolite via Co−O−Si bonds, which is of great importance for the high catalytic activity in both symmetrical and unsymmetrical oxidative thiol coupling reactions. After recycling experiments four times, the CoOx/h-S-1-H used has almost the same chemical state and the same distribution of Co(II) species as the fresh catalysts. Based on DFT calculations and inhibition experiments, the oxidative coupling of thiols undergoes a free radical mechanism in which Co(III) causes RS−H cleavage into RS· and H· species. Subsequently, two RS· radicals are coupled to disulfides, while H· radicals react with the O species to form H2O molecules. This work not only provides guidance on catalyst design and parameter optimization for oxidative thiol coupling but also advances the understanding of the aerobic oxidation mechanism.
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CELLULAR SIGNALLING
·
2024
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IF 4.4
Ziheng Yuan,Genfa Yi,Run Ma,Zhuo Wang,Jihong Hu,Wei Zhao,Ying Hu
Background Aldehyde oxidase 1 (AOX1) is associated with various pathophysiological processes, including cancer. Specifically, AOX1 has been demonstrated to have a close relationship with the progression of certain cancers. However, the expression, function, and mechanisms of action of AOX1 in gallbladder cancer (GBC) remain unclear. Methods Utilizing immunohistochemistry, the study quantified the prevalence of AOX1 within tissues of gallbladder carcinoma and those of the surrounding non-cancerous regions. In vitro assays using gallbladder carcinoma cell lines with modulated AOX1 expression levels were performed to assess the protein's role in cell proliferation, migration, and invasion. Furthermore, flow cytometry techniques were harnessed to determine the influence of AOX1 on the content of reactive oxygen species (ROS) in these cells. Additionally, the expression of epithelial-mesenchymal transition (EMT) markers and the Wnt/β-catenin signaling pathway markersin cells with varied AOX1 expression, detected through Western blot analyses. An in vivo xenograft model involving athymic mice was implemented to explore the influence of AOX1 on gallbladder tumor growth, with Western blot analysis applied to measure EMT marker expression in the resulting tumours. Results Elevated AOX1 protein levels have been observed in gallbladder carcinoma tissues, with such upregulation linked to a negative prognostic outlook for patients. In vitro analyses demonstrate that enhanced AOX1 expression facilitates gallbladder carcinoma cell proliferation, migration, and invasion, while AOX1 suppression yields an inhibitory effect on these cellular behaviors. Western blot results reveal an inverse relationship between AOX1 and E-cadherin levels, yet was positively correlation with N-cadherin, Vimentin, and Snail within both gallbladder cancer cells and in vivo xenograft tumours. Further mechanistic investigation indicates that AOX1 elevation augments reactive oxygen species (ROS) production and initiates the Wnt/β-catenin signaling pathway in these cells. The application of N -acetylcysteine (NAC) and/or KY1797K attenuates the proliferative, migratory, and invasive enhancements imparted by AOX1 overexpression and reinforces these effects when AOX1 is silenced—achieved through ROS mitigation and the obstruction of the Wnt/β-catenin pathway. In vivo studies corroborate these findings, showing AOX1 overexpression to amplify xenograft tumor growth and mesenchymal marker expression, whereas AOX1 interference did the opposite. Conclusions The study indicates that AOX1 functions as a carcinogenic factor in gallbladder carcinoma, enhancing cell proliferation, migration, invasion, and the EMT. These effects are driven by the activation of the Wnt/β-catenin pathway mediated by reactive oxygen species (ROS). Therefore,AOX1 presents potential as a valuable prognostic and diagnostic marker as well as a target for therapeutic intervention in the gallbladder cancer.
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JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Se-Ruo Li,Ning-Ning Kang,Rong-Rong Wang,Meng-Die Li,Li-Hong Chen,Peng Zhou,De-Xiang Xu,Hui Zhao,Lin Fu
Our previous study revealed that 1-nitropyrene (1-NP) exposure evoked pulmonary fibrosis in mice. However, the exact mechanism remained elusive. We found that 1-NP induced telomere damage and cellular senescence in mice lungs, and two alveolar epithelial cells lines. 1-NP downregulated telomere repeat binding factor 2 (TRF2), and upregulated FBXW7. Mechanistically, 1-NP-caused TRF2 ubiquitination and proteasomal degradation depended on E3 ubiquitin ligase activity of FBXW7. Moreover, 1-NP upregulated FBXW7 m6A modification via an ALKBH5-YTHDF1-dependent manner. Further analysis suggested 1-NP promoted ALKBH5 SUMOylation and subsequent proteasomal degradation. Additionally, 1-NP evoked mitochondrial reactive oxygen species (mtROS) overproduction. Mito-TEMPO, a mitochondrial-targeted antioxidant, mitigated 1-NP-caused mtROS overproduction, ALKBH5 SUMOylation, FBXW7 m6A modification, TRF2 degradation, cellular senescence, and pulmonary fibrosis. Taken together, mtROS-initiated ALKBH5 SUMOylation and subsequent FBXW7 m6A modification is indispensable for TRF2 degradation and cellular senescence in alveolar epithelial cells during 1-NP-induced pulmonary fibrosis. Our study provides target intervention measures towards 1-NP-evoked pulmonary fibrosis. Environmental Implication 1-nitropyrene (1-NP) is a representative of nitro polycyclic aromatic hydrocarbon compound that can be formed during the insufficient combustion of organic substances like coal, oil, gas, and tobacco. Moreover, mounting researches have revealed that 1-NP can cause various respiratory system diseases, inclusive of acute lung injury, chronic obstructive pulmonary disease, and allergic asthma. The current research provides evidence that exposure to 1-NP induces pulmonary fibrosis through evoking telomere damage and cellular senescenc. What’s more, Mito-TEMPO, a mitochondrial-targeted antioxidant, can mitigate 1-NP-caused cellular senescence and pulmonary fibrosis. Our study provides target intervention measures towards 1-NP-evoked pulmonary fibrosis.
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CHEMICAL ENGINEERING SCIENCE
·
2024
·
IF 4.1
Yang Xu,Dong Lin,Ze Zong,Qiuming He,Zhe Ma,Xuliang Deng,De Chen,Chaohe Yang,Xiang Feng
Demetalation-metalation method has gained prominence for its higher metal loading, efficiency and shortened synthetic period, particularly in the incorporation of metal atoms with large radii into zeolite framework in recent years. An ammonia-regulated approach has been developed for selectively evolved deep-substituted Ti precursors in dealuminized beta zeolites, which enables a controlled promotion of metalation of Ti atoms. Additionally, this ammonia-regulated approach could also enhance 1-hexene kinetic adsorption by constructing mesopores, which is demonstrated by the lower reaction order of 1-hexene. The synthesized ammonia-regulated N-Ti-beta exhibits rich framework Ti species and a high specific surface area, leading to significantly improved 1-hexene epoxidation performance (conversion of 61%, selectivity of 98%, and a high H 2 O 2 utilization efficiency of 93%). This study not only provides a new strategy to dynamically regulate evolution of Ti precursors by ammonia but also holds promise for advancing related industrial processes in demetalation-metalation methods.
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IEEE SENSORS JOURNAL
·
2024
·
IF 4.3
Xinyue Mao,Wenting Cao,Xinyan Yuan,Xiao Liang,Guanghui Zhang,Yongzhong Wang,Xiaofeng Jin
Acute kidney injury (AKI) is a global health problem due to its high rates of morbidity and mortality. Kidney injury molecule-1 (KIM-1) is considered as a potential biomarker for the diagnosis of AKI. In this study, we proposed an oriented antibody immobilization strategy for reliable detection of KIM-1 by utilizing single-stranded DNA with a methylene blue tag (MB-ssDNA) to achieve directional immobilization of antibodies onto poly methylene blue (PMB) on the electrode surfaces through cyclic voltammetry (CV). PMB not only enhanced electron transfer rate but also provided a platform for the oriented immobilization of antibody. The proposed immunosensor demonstrated a limit of detection (LOD) of 5.8 pg/mL with a linear range from 1 pg/mL to 100 pg/mL. The immunosensor exhibited good repeatability and reusability as well as excellent operational stability. Furthermore, we successfully developed a low-cost and portable paper-based microfluidic electrochemical device to measure KIM-1 concentrations in artificial urine. Integrated flexible electrochemical immunosensors based on antibody orientation immobilization and electrochemical signal amplification have great application potential in personalized point-of-care (POC) clinical diagnosis.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2012
·
IF 2.237
Yi-Qiang Deng,Shuang-Feng Yin,Chak-Tong Au
Series of silicalite-1 catalysts with particle size varying from 50 to 450 nm were synthesized by hydrothermal method using tetrapropylammonium hydroxide as a template and l-lysine as an additive. The crystal size of silicalite-1 could be easily controlled by regulating the amount of l-lysine. The series of silicalite-1 and their NaOH-modified derivatives were examined as catalysts for vapor-phase Beckmann rearrangement of cyclohexanone oxime to caprolactam in the 330–390 °C range. It was found that the crystal size of silicalite-1 has an effect on catalytic activity, ε-caprolactam selectivity, and catalyst stability. With a decline in crystal size, there was improvement of catalytic activity and catalyst stability. It is deduced that silicalite-1, of a smaller crystal size, is less inclined to pore blocking by coke and hence has better resistance to deactivation.
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