ACS Applied Nano Materials
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2024
·
IF 5.3
Rongrong Hu,Wenfang Peng,Lin Cheng,Qiaoyun Wu,Gaolei Dong,Pan Liang,Bobo Yang,Yuefeng Li,Jun Zou,Donghai Feng
Metal halide perovskite nanocrystals (NCs) are emerging for emitting applications due to their high photoluminescence (PL) quantum yield and wide PL wavelength tunability. Here, the light-induced PL enhancement in complexes of CsPbBr3 NCs and 1-octanethiol (OT) molecules (refers to below as NC–OT complexes) is investigated. In comparison with that of as-grown CsPbBr3 NCs, the PL intensity of NC–OT complexes is lower before light illumination. While after ultraviolet light illumination, the PL performance of NC–OT complexes is improved in both air and nitrogen atmospheres. Compared with air atmospheres, anaerobic atmospheres are more effective at increasing the PL intensity because of eliminating photo-oxidation. The light illumination prevents the nonradiative process and enhances the radiative emission in NC–OT complexes, supported by transient PL and photobleaching measurements, due to better surface passivation through thiol–ene reactions. The light-induced PL enhancement in NC–OT complexes provides valuable information for the applications of perovskite nanomaterial-based emitting devices.
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Microorganisms
·
2024
·Vol. 12
·期 7
·1381
·
IF 4.1
Xuezhi Shao,Hongliang Fang,Tao Li,Liyan Yang,Dengfeng Yang,Lixia Pan
Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that catalyze the oxidative cleavage of recalcitrant polysaccharides. There are limited reports on LPMOs capable of concurrently catalyzing the oxidative cleavage of both cellulose and chitin. In this study, we identified and cloned a novel LPMO from the newly isolated bacteriumChitinilyticum aquatileCSC-1, designated asCaLPMO10. When using 2, 6-dimethylphenol (2, 6-DMP) as the substrate,CaLPMO10 exhibited optimal activity at 50 °C and pH 8, demonstrating good temperature stability at 30 °C. Even after a 6 h incubation at pH 8 and 30 °C,CaLPMO10 retained approximately 83.03 ± 1.25% residual enzyme activity. Most metal ions were found to enhance the enzyme activity ofCaLPMO10, with ascorbic acid identified as the optimal reducing agent. Mass spectrometry analysis indicated thatCaLPMO10 displayed oxidative activity towards both chitin and cellulose, identifying it as a C1/C4-oxidized LPMO.CaLPMO10 shows promise as a key enzyme for the efficient utilization of biomass resources in future applications.
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ANALYTICAL LETTERS
·
2024
·
IF 1.6
Jiali Ju,Qiaoyu Ding,Jianwei Xie,Guixin Li
A graphene oxide-Ru(bpy)32+-polyaniline-nanocomposite (GO-Ru(bpy)32+-PANI) was prepared to develop a sensitive label-free electrochemiluminescence (ECL) immunosensor for mucoprotein 1 (MUC1). Ru(bpy)32+ and PANI were decorated together on graphene oxide for the first time. The GO-Ru(bpy)32+-PANI material was characterized by scanning electron microscopy (SEM), absorption spectroscopy, Fourier transform infrared spectroscopy (FTIR), fluorescence, electrochemical impedance spectroscopy (EIS), and ECL. The GO-Ru(bpy)32+-PANI exhibited excellent stability. The ECL immunosensor for MUC1 was fabricated by covalently immobilizing anti-MUC1 upon GO-Ru(bpy)32+-PANI modified indium-doped tin oxide (ITO) electrode. In the presence of MUC1, a decrease of ECL was observed due to the formation of the anti-MUC1/MUC1 complex. The immunosensor showed a linear relationship from 1 × 10−13 to 1 × 10−7 mg/mL with a detection limit of 8.2 × 10−14 mg/mL. The immunosensor was applied to the determination of MUC1 in human serum.
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Molecular Catalysis
·
2024
·
IF 3.9
Yan-Fei Liang,Shuai Feng,Dan Wu,Meng Ma,Jin-Tao Ren,Qian Zhao,Wen-Yun Gao,Heng Li
1-Deoxy- D- xylulose-5-phosphate synthase (DXS) is the first rate-limiting enzyme in the 2-methyl- D- erythritol-4-phosphate (MEP) terpenoid biosynthetic pathway. To explore the active sites of the protein, we made a series of mutations to the key amino acid residues of Escherichia coli DXS including H49, R420, D427, and R478. The results show that several mutants including H49Q lose almost all their catalytic activity in DXP synthesis, but the double-point mutants H49QD427H and H49QD427 N recover 100 % activity of the wild type enzyme. The kinetic characterization displays that the affinity of the double-point mutants for pyruvate and D- glyceraldehyde-3-phosphate only slightly decreases. We also assessed impact of the mutation on the oxidative decarboxylation activity of all the mutants. Despite the fact that H49QD427H and H49QD427 N get back the full activity with regard to DXP synthesis, their oxidase activity remains unrestored. Furthermore, we assayed the acceptor substrate spectrum of the mutants and the results show that a large portion of them catalyzes ligation between pyruvate and nitrosobenzene to form N-phenyl-N-hydroxyacetamide, whereas R420D, R420F, and D427H exhibit activity in connection of pyruvate and benzaldehyde to afford phenylacetocarbinol (PAC), with the first two mutants yielding ( S )-PAC (yields 21 %-25 %, ee values 71–75 %) while D427H furnishing ( R )-PAC (yield 41 %, ee value 91 %), implying the key role of D427 for the acceptor substrate recognition and the stereoselectivity of the enzyme. It is worth noting that this is the first report of DXS mutants that can recognize benzaldehyde as an acceptor substrate and generate both enantiomers of PAC.
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IMMUNOLOGY LETTERS
·
2025
·
IF 2.8
Xing Chen,Lixiang Li,Yan Deng,Juan Liao,Hui Meng,Limei Liang,Jie Hu,Dongwei Xie,Guizi Liang
(1) Background Metabolic abnormalities and immune inflammation are key elements within pathogenesis of pulmonary arterial hypertension (PAH). And in PAH patients, aberrant glutamine metabolism has been observed; however, the function of glutaminase 1 (GLS1) in macrophage is still unknown. So we aims to investigate GLS1′s impact upon macrophages in PAH. (2) Methods We firstly constructed an monocrotaline (MCT)-induced PAH rat model. Briefly, the PAH rats were treated with the GLS1 inhibitor BPTES, and various index were evaluated, including hemodynamics, right ventricular function, pulmonary vascular remodeling, macrophage markers, and glutamine metabolism. After that, we polarized bone marrow-derived macrophages (BMDMs) into M1 phenotype and then subjected to BPTES intervention. Finally, we assessed macrophage phenotype, inflammatory markers, and glutamine metabolism indicators, along with the impact of BMDM supernatant on the behavior of pulmonary arterial smooth muscle cells (PASMCs). (3) Results : GLS1 was significantly upregulated in both PAH patients and rats. Treatment with the GLS1 inhibitor BPTES markedly improved pulmonary arterial pressure, right ventricular function, and pulmonary vascular remodeling in PAH rats, while inhibiting M1 macrophage polarization, NLRP3 activation, and the release of pro-inflammatory cytokines. This, in turn, alleviated the proliferation and migration of PASMCs induced by inflammatory stimuli. (4) Conclusion : We propose that targeting GLS1 to reduce M1 macrophage polarization and inflammatory responses may represent a promising therapeutic approach for PAH.
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
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2024
·
IF 3
Zhang Cong,Guo Yan,Xiao Libai,Gao Hongxu,Zhao Fengqi,Huang Jie,Lü Xingqiang,Ma Haixia
Three 4-(1 H -tetrazole-5-yl-amino)-1,2,4,5-tetrazine-1-one (TATzO) metal salts, K 2 (TATzO)(H 2 O) ( 1 ) and Mg(TATzO)(H 2 O) 6 ·2H 2 O ( 2 ) and Sr(TATzO)(H 2 O) 4 ( 3 ), were synthesized and characterized using Fourier transform infrared (FTIR), elemental analysis, single-crystal X-ray diffraction, and powder X-ray diffraction patterns. The crystal structure and hydrogen bonding interactions have been analyzed. It turns out that 1 and 3 crystallize into a three-dimensional topological network and have strong π – π stacking and hydrogen bonding interactions. Furthermore, the geometric optimization of 1 – 3 was performed using B3LYP-D3/def2svp in Gaussian 16 software package and then analyzed by reduced density gradient. The thermal behavior was conducted using differential scanning calorimetry and thermogravimetric (TG). The decomposition temperature of the three complexes exceeds 250 °C, much higher than that of TATzO (230 °C). The thermal decomposition products of 1 were further studied using thermogravimetric-Fourier transform infrared-mass spectrometry (TG/FTIR/MS). The impact sensitivity test indicated that making complexes can help decrease the sensitivity of the original energetic ligand. Graphical abstract
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CHEMICAL ENGINEERING SCIENCE
·
2024
·
IF 4.1
Lei Wu,Menghan Liu,Yuting Chen,Ming Li,Tingting Yin,Cong Ma,Wei Fan
UF is widely used as a wastewater treatment and recycling technology. However, membrane fouling is still an important factor affecting the wide application. In this study, APIm with positive amino group was adsorbed to the surface of the carboxylated polyacrylonitrile (PAN) membrane by electrostatic force to reduce the fouling of sodium alginate (SA) caused by Ca 2+ . The results show that 1 v/v% APIm was selected as the optimal dosage, accompanied by the highest water flux of 330.5 L·m -2 h −1 bar −1 and bovine serum albumin (BSA) rejection rate of 91.0 %. When the concentration of Ca 2+ was increased to 10 mM, the Ca 2+ catch amount increased to 48.25 mg/cm 2 . The main anti-fouling mechanism was that APIm captured a certain amount of Ca 2+ in situ, destroyed the complex structure of sodium alginate (SA) and Ca 2+ . Then the fouling layer was easy to be removed by physical cleaning. At the concentration of 10 mM Ca 2+ , the membrane flux recovery rate of 1 v/v% APIm membrane was increased to 80.1 %. This study demonstrates a fouling control strategy for in-situ removal of Ca 2+ bound SA fouling, providing a new approach for the future development of UF membrane.
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IEEE SENSORS JOURNAL
·
2024
·
IF 4.3
Xinyue Mao,Wenting Cao,Xinyan Yuan,Xiao Liang,Guanghui Zhang,Yongzhong Wang,Xiaofeng Jin
Acute kidney injury (AKI) is a global health problem due to its high rates of morbidity and mortality. Kidney injury molecule-1 (KIM-1) is considered as a potential biomarker for the diagnosis of AKI. In this study, we proposed an oriented antibody immobilization strategy for reliable detection of KIM-1 by utilizing single-stranded DNA with a methylene blue tag (MB-ssDNA) to achieve directional immobilization of antibodies onto poly methylene blue (PMB) on the electrode surfaces through cyclic voltammetry (CV). PMB not only enhanced electron transfer rate but also provided a platform for the oriented immobilization of antibody. The proposed immunosensor demonstrated a limit of detection (LOD) of 5.8 pg/mL with a linear range from 1 pg/mL to 100 pg/mL. The immunosensor exhibited good repeatability and reusability as well as excellent operational stability. Furthermore, we successfully developed a low-cost and portable paper-based microfluidic electrochemical device to measure KIM-1 concentrations in artificial urine. Integrated flexible electrochemical immunosensors based on antibody orientation immobilization and electrochemical signal amplification have great application potential in personalized point-of-care (POC) clinical diagnosis.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2011
·
IF 2.089
Baimei Yang,Hui Wan,Guofeng Guan
Isobaric binary vapor–liquid equilibrium (VLE) data for 1-butanol + ethylbenzene, or o-, or m-, or p-xylene and isobaric quinary VLE data for 1-butanol + ethylbenzene + o-xylene + m-xylene + p-xylene are measured at 101.33 kPa using a modified Rose cell with circulation of both phases. The four binary systems of 1-butanol + ethylbenzene, or o-, or m-, or p-xylene exhibit minimum boiling azeotropes, and the composition of the azeotropes are reported. The VLE data for the four binary systems are checked to meet rigorous thermodynamic consistency by Herington method and the point-to-point test of the Fredenslund method. The nonideality in vapor phase of the measured binary systems is analyzed through calculating fugacity coefficients. Combined with the Hayden–O'Connell (HOC) equation, the VLE data for 1-butanol + ethylbenzene, or o-, or m-, or p-xylene are well-correlated by the nonrandom two-liquid (NRTL), universal quasichemical activity coefficient (UNIQUAC), and Wilson equations. The NRTL model parameters obtained from correlation are used to predict the VLE data of the quinary system. According to the average absolute deviation values, the obtained quinary predicted values are in good agreement with the experimental values.
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Soft Matter
·
2011
·Vol. 7
·期 11
·5200-5207
·
IF 4.457
Xi-Lian Wei,Zeng-Bin Wei,Xiu-Hong Wang,Zhong-Ni Wang,De-Zhi Sun,Jie Liu,Huai Hua Zhao
The aqueous mixtures of an anionic surfactant (sodium dodecyl benzene sulfonate, SDBS) and an ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, [Bmim]BF4) within a certain range of compositions can spontaneously separate into two immiscible aqueous phases (ATPS): one surfactant-rich and the other ionic liquid-rich. The phase diagrams of the ternary system of the SDBS/[Bmim]BF4/water were drawn based on experimental data determined at 10.0, 20.0 and 30.0 °C, respectively. The borderlines of the different specific regions in the diagrams were determined using turbidity titration method. The narrow two-phase region aqueous were found to be close to the water - [Bmim]BF4 side. The formation conditions, compositions, and physicochemical properties of the aqueous two phases have been studied. The effects of temperature, surfactant and ionic liquid content on the phase separation time and phase volume ratio of the ATPS were also investigated. This phase separation phenomenon was found to be likely due to the existence of micelle aggregates with quite large size obtained by Transmission Electron Microscopy (TEM) and Steady-state Fluorescence Quenching Measurements (SFQM) methods.
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CELLULAR SIGNALLING
·
2024
·
IF 4.4
Ziheng Yuan,Genfa Yi,Run Ma,Zhuo Wang,Jihong Hu,Wei Zhao,Ying Hu
Background Aldehyde oxidase 1 (AOX1) is associated with various pathophysiological processes, including cancer. Specifically, AOX1 has been demonstrated to have a close relationship with the progression of certain cancers. However, the expression, function, and mechanisms of action of AOX1 in gallbladder cancer (GBC) remain unclear. Methods Utilizing immunohistochemistry, the study quantified the prevalence of AOX1 within tissues of gallbladder carcinoma and those of the surrounding non-cancerous regions. In vitro assays using gallbladder carcinoma cell lines with modulated AOX1 expression levels were performed to assess the protein's role in cell proliferation, migration, and invasion. Furthermore, flow cytometry techniques were harnessed to determine the influence of AOX1 on the content of reactive oxygen species (ROS) in these cells. Additionally, the expression of epithelial-mesenchymal transition (EMT) markers and the Wnt/β-catenin signaling pathway markersin cells with varied AOX1 expression, detected through Western blot analyses. An in vivo xenograft model involving athymic mice was implemented to explore the influence of AOX1 on gallbladder tumor growth, with Western blot analysis applied to measure EMT marker expression in the resulting tumours. Results Elevated AOX1 protein levels have been observed in gallbladder carcinoma tissues, with such upregulation linked to a negative prognostic outlook for patients. In vitro analyses demonstrate that enhanced AOX1 expression facilitates gallbladder carcinoma cell proliferation, migration, and invasion, while AOX1 suppression yields an inhibitory effect on these cellular behaviors. Western blot results reveal an inverse relationship between AOX1 and E-cadherin levels, yet was positively correlation with N-cadherin, Vimentin, and Snail within both gallbladder cancer cells and in vivo xenograft tumours. Further mechanistic investigation indicates that AOX1 elevation augments reactive oxygen species (ROS) production and initiates the Wnt/β-catenin signaling pathway in these cells. The application of N -acetylcysteine (NAC) and/or KY1797K attenuates the proliferative, migratory, and invasive enhancements imparted by AOX1 overexpression and reinforces these effects when AOX1 is silenced—achieved through ROS mitigation and the obstruction of the Wnt/β-catenin pathway. In vivo studies corroborate these findings, showing AOX1 overexpression to amplify xenograft tumor growth and mesenchymal marker expression, whereas AOX1 interference did the opposite. Conclusions The study indicates that AOX1 functions as a carcinogenic factor in gallbladder carcinoma, enhancing cell proliferation, migration, invasion, and the EMT. These effects are driven by the activation of the Wnt/β-catenin pathway mediated by reactive oxygen species (ROS). Therefore,AOX1 presents potential as a valuable prognostic and diagnostic marker as well as a target for therapeutic intervention in the gallbladder cancer.
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JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Se-Ruo Li,Ning-Ning Kang,Rong-Rong Wang,Meng-Die Li,Li-Hong Chen,Peng Zhou,De-Xiang Xu,Hui Zhao,Lin Fu
Our previous study revealed that 1-nitropyrene (1-NP) exposure evoked pulmonary fibrosis in mice. However, the exact mechanism remained elusive. We found that 1-NP induced telomere damage and cellular senescence in mice lungs, and two alveolar epithelial cells lines. 1-NP downregulated telomere repeat binding factor 2 (TRF2), and upregulated FBXW7. Mechanistically, 1-NP-caused TRF2 ubiquitination and proteasomal degradation depended on E3 ubiquitin ligase activity of FBXW7. Moreover, 1-NP upregulated FBXW7 m6A modification via an ALKBH5-YTHDF1-dependent manner. Further analysis suggested 1-NP promoted ALKBH5 SUMOylation and subsequent proteasomal degradation. Additionally, 1-NP evoked mitochondrial reactive oxygen species (mtROS) overproduction. Mito-TEMPO, a mitochondrial-targeted antioxidant, mitigated 1-NP-caused mtROS overproduction, ALKBH5 SUMOylation, FBXW7 m6A modification, TRF2 degradation, cellular senescence, and pulmonary fibrosis. Taken together, mtROS-initiated ALKBH5 SUMOylation and subsequent FBXW7 m6A modification is indispensable for TRF2 degradation and cellular senescence in alveolar epithelial cells during 1-NP-induced pulmonary fibrosis. Our study provides target intervention measures towards 1-NP-evoked pulmonary fibrosis. Environmental Implication 1-nitropyrene (1-NP) is a representative of nitro polycyclic aromatic hydrocarbon compound that can be formed during the insufficient combustion of organic substances like coal, oil, gas, and tobacco. Moreover, mounting researches have revealed that 1-NP can cause various respiratory system diseases, inclusive of acute lung injury, chronic obstructive pulmonary disease, and allergic asthma. The current research provides evidence that exposure to 1-NP induces pulmonary fibrosis through evoking telomere damage and cellular senescenc. What’s more, Mito-TEMPO, a mitochondrial-targeted antioxidant, can mitigate 1-NP-caused cellular senescence and pulmonary fibrosis. Our study provides target intervention measures towards 1-NP-evoked pulmonary fibrosis.
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