LANGMUIR
·
2026
·
IF 4.4
Yong Wang,Chunxiao Yan,Weiye Liang,Qiang Zhang,Gang Cheng,Jicheng Dong,Lin Jin,Huan Tang,Fang Cheng,Lijun Zhang
Orthokeratology (OK) lenses for myopia correction are susceptible to biofouling by tear-derived biomolecules, escalating the risks of ocular infection and inflammation. Prevailing studies that rely on end point protein quantification fail to capture the real-time kinetics of fouling formation. Here, we fabricated a UV pressure-assisted polymer-grafted quartz crystal microbalance with dissipation monitoring (QCM-D) sensor exhibiting exceptional stability, nanoscale smoothness (RMS roughness ≈2 nm), and interfacial peel resistance. This platform enables in situ tracking of adsorption/desorption kinetics for four critical tear components: native/denatured lysozyme and oxidized/native lecithin. Key findings reveal a flow-dependent fouling behavior, wherein low flow rates increase biomolecule adsorption by 37–80% compared with higher flows. We further identify denatured lysozyme and native lecithin as resilient contaminants characterized by a stronger deposition affinity and pronounced resistance to elution. Quantitative screening of multipurpose solutions (MPSs) demonstrates that MPS #2 achieves 20–100% elution rate across biomolecules, outperforming commercial benchmarks. By leveraging dissipation–frequency (D–F) analysis, we clarify the fundamental mechanisms of biofouling formation at the molecular level. Collectively, this work establishes three critical advances: (1) a real-time biofouling diagnostic platform for OK lens interfaces, (2) molecular design principles for antifouling materials based on adhesion remodeling theory, and (3) an accelerated MPS formulation screening paradigm for ocular device safety.
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高影响因子
Applied Food Research
·
2026
·Vol. 6
·期 2
·102705
·
IF 5.4
Hong Li,Yanyan Li,Ran Ding,Xintian Qiao,Zihao Guo,Ruiling Shen,Huiling Mu,Zheng Guo
A novel phospholipid derivative, PA-CG, is synthesized via a PLD-catalyzed method. • Success of PA-CG synthesis is structurally confirmed by NMR and MS analysis. • PA-CG shows superior antioxidant activity to PC and CG in DPPH, ABTS, and FRAP assays. • SPI+PA-CG combination improves the physical and oxidative stability of O/W emulsions. This work presents an efficient enzymatic synthesis of a novel phosphatidyl caffeoyl glycerol (PA-CG) via phospholipase D (PLD)-catalyzed transphosphatidylation of phosphatidylcholine (PC) with caffeoyl glycerol (CG). Under optimized conditions (CG/PC 4:1 mol/mol, water/methyl tert-butyl ether (MTBE) 0.15:1 V/V, PLD 30 U/mL, 40 °C, 3 h), the yield exceeded 95% (HPLC mol% relative to PC), and the product was confirmed by NMR and ESI-MS. PA-CG exhibited additive antioxidant activity over PC and CG in DPPH, ABTS, and FRAP assays. When combined with soy protein isolates (SPI), PA-CG outperformed SPI or SPI+PC in stabilizing oil-in-water (O/W) emulsions, evidenced by the highest emulsifying activity index, smallest and most homogeneous droplets, and lowest creaming index, attributed to its excellent amphiphilic interfacial properties. Moreover, SPI+PA-CG emulsion displayed the slowest increase in peroxide value (POV) and thiobarbituric acid reactive substances (TBARS) over time. Collectively, PA-CG is a promising dual-function antioxidant and emulsifier for food, cosmetic, and pharmaceutical applications. Download: Download high-res image (293KB) Download: Download full-size image
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Ming Wang,Yanqing Liu,Xingrui Liu,Pengkun Li,Xingjian Song,Lin Yang,Xiang Sui,Mengyao Tang,Qiang Wang,Shaowei Chen
Hydrothermal synthesis of Bi-Bi 3 TaO 7 heterostructures with mannitol • Morphological evolution from nanoflakes to nanoparticles • Enhanced visible absorption and charge separation by modulation of d-band center • Photocatalytic performance of nitrogen fixation up to 10 times that of pristine Bi 3 TaO 7 Morphology control can effectively regulate the interfacial electric field and electronic structure of photocatalytic materials, making it an important strategy for achieving efficient nitrogen fixation. In this study, a facile one-step hydrothermal method is used, with mannitol as both a reducing agent and morphology regulator, to in situ deposit metallic bismuth nanoparticles onto the surface of Bi₃TaO₇ (BTO), forming Bi-Bi₃TaO₇ (Bi-BTO) Schottky heterojunctions. The Bi-BTO samples consist of nanoparticles as compared to nanosheets for pure BTO, which not only increases the specific surface area and accessibility of catalytic active sites but also effectively regulates the interfacial contact of the Schottky heterojunction. This strengthens the built-in electric field and shifts the d-band center closer to the Fermi level. The optimized electronic structure enhances orbital coupling between the catalyst and nitrogen molecules, promoting nitrogen adsorption and activation and lowering the energy barrier for the formation of the rate-determining *NNH intermediate. Thanks to the synergistic effect of morphology engineering on the built-in electric field and d-band center, the optimal sample Bi-BTO-2 shows an excellent photocatalytic nitrogen fixation performance even without a hole scavenger, achieving an ammonia generation rate of 195 μmol·g −1 ·h −1 , 10 times that of pure BTO, with ammonia selectivity approaching 100%. This study highlights the key role of morphology engineering in regulating the interfacial properties and electronic structure of Schottky heterojunctions, providing a practical modification strategy for the design and engineering of high-performance photocatalysts for nitrogen fixation. Download: Download high-res image (176KB) Download: Download full-size image
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BIOORGANIC CHEMISTRY
·
2026
·
IF 5.1
Qian Zhang,Wenyu Wang,Chenhan Wu,Qingting Du,Weili Yin,Jiaheng Chen,Jian Ma,Chong Li,Shaojie Zhang
Ulcerative colitis (UC) is characterized by recurrent inflammation of the gastrointestinal tract, and currently available treatment drugs are limited. Plant-derived polysaccharides hold promise for UC prevention. A water-soluble α -D-glucan (CLP80–1) with a molecular weight of 8.17 × 10 5 Da was isolated and characterized from the roots of traditional Chinese medicinal plant Crepis lignea . Structural analysis revealed that its repeating units comprise →6)- α -D-Glc p -(1 → and →3,6)- α -D-Glc p- (1 → residues as the backbone, with α -D-Glc p -(1 → as side chains substituted at C-3. The protective effects of CLP80–1 on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in Drosophila melanogaster were investigated. Our results demonstrated that treatment with 1% CLP80–1 significantly improved survival rates, attenuated gut atrophy, reduced intestinal permeability, enhanced intestinal barrier function, suppressed excessive proliferation of intestinal stem cells (ISCs) and enteroblasts (EBs), and inhibited the death of intestinal epithelial cells (IECs). Metabolomic and transcriptomic analyses revealed that CLP80–1 mainly affected amino acid metabolism, TCA cycle, oxidative phosphorylation and JAK/STAT signaling pathway. Furthermore, CLP80–1 reduced reactive oxygen species (ROS) levels, suppressed the expression of pro-inflammatory factors, and downregulated the JAK-STAT signaling pathway, thereby mitigating oxidative stress and inflammatory responses. These findings suggest that CLP80–1 is effective in alleviating intestinal inflammation and may serve as a promising therapeutic candidate for addressing gut inflammatory conditions.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2025
·
IF 4.7
Zhiqiang Yu,Yufan Lan,Tenghui Li,Shucheng Huang,Wuli Han,Xiao Luo
This study constructed an S-scheme MIL( Fe )DCA (et al., 2023b (6)) /MoS 2 heterojunction for enhanced performance in degrading pollutants under a wide spectrum, benefiting from the customized d-band centers (by the built-in electric field) and the Mars–Van Krevelen cycle. Results reveal the tetracycline degradation rate of MIL( Fe )DCA (et al., 2023b (6)) /MoS₂ (~95 %) increases 1.38-fold and 11.8-fold compared to pristine MIL-Fe-DCA and MoS 2 , respectively, with better repeatability.Also, satisfactory reduction of Cr(VI) ions (75 %) and removal of coumarin (81.3 %) and ciprofloxacin hydrochloride (83 %) are observed. The catalyst exhibits rich defects, wide-spectrum solar light harvesting, efficient separation of h + ~ e − pairs and a robust redox process. It is found that the charge transfer in the S-scheme interfacial electric field elevates the d-band center towards the Fermi level, which can effectively lower water activation energy to more easily produce •OH, enhancing intermediate adsorption on the catalyst's surface, thus facilitating charge transfer and accelerating reaction kinetics. Another major emphasis is the oxygen defects induced Mars–Van Krevelen cycle, wherein adsorbed reactants, lattice oxygen, h + , •O 2 − , Fe 2+ and Fe 3+ participate in the reaction processes, which contributes to the photo-thermal conversion, achieving the synergy between photocatalysis and photothermal catalysis. This work offers a synergetic strategy involving the interfacial electric field, d-band center, and photo-thermal effect for photocatalytic water purification.
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TALANTA
·
2026
·
IF 6.7
Dan Wang,Panpan Chen,Qin Huang,Yan Ma,Xueping Tao,Yuanmin Gong,Jiaping Cui,Yang Zhou,Zhining Xia,Qifeng Fu
The inherently low phase ratio and limited surface capacity of open-tubular capillary electrochromatography (OT-CEC) significantly restrict its efficiency in enantioseparation. To overcome this limitation, we developed a rigid-flexible composite interface strategy to construct a high-efficiency chiral ligand-exchange CEC (CLE-CEC) column. A uniform nanocrystalline UiO-66-NH 2 layer was in situ grown on the capillary inner wall to serve as a high-surface-area rigid scaffold, followed by the covalent immobilization of L-arginine (L-Arg) as a flexible chiral ligand. This composite architecture combines the structural stability of metal-organic frameworks (MOFs) with the stereochemical selectivity of amino acid ligands. The dense MOF scaffold effectively increases the specific surface area and ligand loading capacity of the stationary phase, thereby facilitating efficient Zn(II)-mediated ternary complexation for chiral recognition. Validating this design, the UiO-66-NH-L-Arg@capillary column achieved baseline separation for seven pairs of Dns-D,L-AAs, with resolution factors reaching up to 2.56. The column exhibited satisfactory repeatability and good operational stability. Furthermore, the practicability of this method was demonstrated by its successful application in the kinetic monitoring of L-alanine dehydrogenase, providing a robust micro-separation tool for precise bioanalysis.
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APPLIED SURFACE SCIENCE
·
2026
·
IF 6.6
Wenmei Wuxia,Yu Chen,HuanHuan Li,Ning Ma,Lengyuan Niu,Yinyan Gong,Can Li
Zr 4+ incorporation drives a complete transition to an amorphous phase and increases the electrochemically active surface area. • Zr 4+ incorporation induces a downward shift in the core-level binding energies of Fe, Co, and Ni sites. • Optimized S-CrMnFeCoNiZr 1.5 specimen achieves overpotentials of 240/287 mV at 10/100 mA/cm 2 . • S-CrMnFeCoNiZr 1.5 specimen exhibits outstanding stability and a 16-fold enhancement in TOF relative to S-CrMnFeCoNi specimen. Developing efficient and durable electrocatalysts for oxygen evolution reaction (OER) is pivotal to advancing renewable energy conversion technologies. Herein, a series of amorphous CrMnFeCoNi-based high-entropy compounds (HECs) with varied Zr content were synthesized, and the role of Zr doping in modulating the atomic/electronic structures were investigated, as well as the OER activity. Experimental characterization reveals that Zr 4+ incorporation drives a complete transition to an amorphous phase, increases the electrochemically active surface area, and induces a downward shift in the core-level binding energies of Fe, Co, and Ni. Relative to the Zr-free analogue, the optimized catalyst S-CrMnFeCoNiZr 1.5 delivers a 57/69 mV reduction in overpotential at 10/100 mA cm −2 , exhibits a 17-fold higher turnover frequency, and maintains exceptional operational stability over 72 h. This OER activity outperforms most previously reported HECs. Density functional theory calculations corroborate these observations, demonstrating that Zr doping upshifts the d-band centers of Fe, Co, and Ni, thereby strengthening adsorption of intermediates and optimizing the free-energy profile for the rate-determining steps. Collectively, this study establishes a rigorous structure–electronic property–performance correlation and demonstrates Zr-mediated electronic modulation as a rational, scalable strategy for engineering high-entropy electrocatalysts. Download: Download high-res image (70KB) Download: Download full-size image
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MOLECULAR & CELLULAR PROTEOMICS
·
2026
·
IF 6.3
Hongyu Zhang,Baojin Zhou,Jingwen Liu,Yan Ren
Background and aim Platycodin D (PD), a major bioactive saponin isolated from the traditional Chinese medicine (TCM) Platycodon grandiflorus , has shown promising therapeutic potential against non-small cell lung cancer (NSCLC). However, the functional mechanisms of PD in NSCLC progression remains unclear. This study aimed to explore the pharmacological mechanism of PD against NSCLC. Materials and methods Thermal proteome profiling (TPP) approach, molecular docking, cellular thermal shift assay (CETSA) and peptide-centric local stability assay (PELSA) were employed to identify the potential binding target of PD. Subsequent Western Blot and immunoprecipitation-Western Blot (IP-WB) experiments were conducted to investigate the downstream signaling pathways of the target. Furthermore, proteomic and ubiquitinomic profiling of PD-treated cells were performed to investigate its functions on global. Results Replication factor C subunit 4 (RFC4) was identified as a potential binding target of PD by TPP and their binding sites were further exposed by PELSA. PD-RFC4 complex promotes the degradation of Notch1 and Notch3 by reducing nuclear entry of their domains. Compared with control treatment, the differentially expressed proteins induced by PD were found to be primarily involved in ferroptosis, ubiquitination, platinum drug resistance and ribosome-related processes. The ubiquitin proteome analysis revealed that proteins associated with the Notch pathway underwent ubiquitin modifications. Conclusions PD binds to RFC4 and inhibits its activity, leading to downregulation of the Notch signaling pathway, ultimately triggering cancer cell apoptosis. PD is a natural product with potential therapeutic value for NSCLC.
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INORGANIC CHEMISTRY
·
2023
·
IF 4.6
Yu-Qing Yang,Shen-Jing Ji,Nian-Tzu Suen
Reported are the synthesis, material characterization, and electrocatalytic hydrogen evolution reaction (HER) in acid and alkaline electrolytes for the Brewer intermetallic phase, Nb6Co7 and Mo6Co7. It was realized that the overpotential at a current density of 10 mA/cm2 (η10) for Nb6Co7 (η10 = 62 mV) and Mo6Co7 (η10 = 143 mV) are both much lower than that of using a single Co metal (η10 = 253 mV) in alkaline electrolytes. The enhancement of electrocatalytic HER activity of Nb6Co7 and Mo6Co7 can be attributed to the hypo-hyper-d-electronic interaction between Nb/Mo and Co elements. Based on the result of density functional theory calculation, alloying between Nb/Mo and Co elements will increase the antibonding state population of the Co–Co bond near the Fermi level (EF), which induces the synergistic effect to influence the adsorption energy of the H atom (ΔGH) on the surface of Nb6Co7 and Mo6Co7. Moreover, the role of the Nb element is not only a simple electron donor but is also an anchor position for the OH molecule (i.e., dual function) due to the bonding character of the Nb–Co bond near EF. It can reduce the OH position effect as well as the activation energy for water dissociation, which rationalizes the high and robust HER performance of Nb6Co7 to that of commercial Pt/C (η10 = 67 mV) in alkaline electrolytes.
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高影响因子
Journal of the Mechanical Behavior of Biomedical Materials
·
2023
·
IF 3.9
Wanzhen Li,Yongqi Yang,Jun Wang,Ting Ge,Shuixia Wan,Lin Gui,Yugui Tao,Ping Song,Liangjun Yang,Fei Ge,Weiwei Zhang
At present, simple anti-tumor drugs are ineffective at targeting bone tissue and are not purposed to treat patients with bone metastasis. In this study, zoledronic acid (ZOL) demonstrated excellent bone-targeting properties as a bone-targeting ligand. The metal-organic framework (MOF) known as ZIF-90 was modified with ZOL to construct a bone-targeting-based drug delivery system. Chlorin e6 (Ce6) was loaded in the bone-targeted drug delivery system and combined with 2-deoxy-D-glucose (2-DG), which successfully treated bone tumors when enhanced photodynamic therapy was applied. The Ce6@ZIF-PEG-ZOL (Ce6@ZPZ) nanoparticles were observed to have uniform morphology, a particle size of approximately 210 nm, and a potential of approximately −30.4 mV. The results of the bone-targeting experiments showed that Ce6@ZPZ exhibited a superior bone-targeted effect when compared to Ce6@ZIF-90-PEG. The Ce6@ZPZ solution was subjected to 660 nm irradiation and the resulting production of reactive oxygen species increased over time, which could be further increased when Ce6@ZPZ was used in combination with 2-DG. Their combination had a stronger inhibitory capacity against tumor cells than either 2-DG or Ce6@ZPZ alone, increasing the rate of tumor cell apoptosis. The apoptosis rate caused by HGC-27 was 61.56% when 2-DG was combined with Ce6@ZPZ. In vivo results also showed that Ce6@ZPZ combined with 2-DG maximally inhibited tumor growth and prolonged mice survival compared to the other experimental groups. Therefore, the combination of PDT and glycolytic inhibitors serves as a potential option for the treatment of cancer.
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JOURNAL OF POLYMER SCIENCE
·
2023
·Vol. 61
·期 19
·2303-2315
·
IF 3.4
Yipeng Chen,Jiangang Zhang,Yuesheng Zhang,Wen Cao,Xiong Liu,Jianna Bao,Xianming Zhang,Wenxing Chen
Copolymerizing poly(lactide) with other materials to obtain better comprehensive performance is a effective way to expand its application range. In this work, the precursors of hydroxyl terminated (poly(L-lactide) [PLLA], poly(ε-caprolactone) [PCL], poly(D,L-lactide) [PDLLA]) were prepared, and PLLA-PCL-PDLLA copolymer were synthesized by chain extension. The effects of the proportion and molecular weight of each component and the amount of chain extender on crystallization, phase structures, mechanical properties and thermal stabilities of PLLA-PCL-PDLLA copolymer were studied in detail. Based on small-angle X-ray scattering results, the competition between crystallization and microphase separation was regulated by the composition and chain length of prepolymers. As the ratio of PLLA/PDLLA was 1:1, crystallization was prevailing and no obvious peak was observed in SAXS pattern. The tensile test results showed that as the ratio of PLLA/PDLLA increased from 1:1 to 1:5, the elongation at break of the copolymer changed from 1.8% to 343%. By using shorter length of PCL and PLLA segments in chain extension, improvement in strength and flexibility were obtained due to moderate degree of crystallization and microphase separation. This work used biodegradable materials to prepare extraordinary toughness copolymers without losing the biocompatibility, which may provide a feasible method for obtaining high toughness and biodegradable PLA-based materials. Graphical
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2022
·Vol. 45
·期 6
·1075-1088
·
IF 3.434
Wei Kaixin,Wu Xiaomei,Ma Baodi,Li Zhi,Xu Yi
A series of nickel-incorporated SBA-15 mesoporous molecular sieves (Ni-SBA-15) were prepared as support for the immobilization of his-tagged recombinant Microbacterium esterase. The Ni-SBA-15 could strongly and specific absorb the his-tagged esterase from cell disrupted supernatant. It was found that the nickel amount in Ni-SBA-15 has dramatic influence on the activity and thermo-stability of immobilized enzyme, while the kinds of nickel precursor had little effect on enzyme stability. The morphology, chemical composition and structure of the best support NiCl 2 -SBA-15 (Ni-SBA-15 prepared from NiCl 2 precursor) were characterized by various spectroscopy techniques. The immobilized esterase retained full activity of free esterase and showed high immobilized yield (> 90%) with higher thermo-stability, pH stability and organic solvent resistance compared with free enzyme. The optimum reaction temperature increased from 35 to 40 °C and the optimal reaction pH moved from 10.0 to 8.0 after enzyme immobilization. The immobilized esterase exhibited excellent storage stability and keeping 92% of the initial activity after 30 days’ storage at 25 °C. In addition, the immobilized esterase had excellent reusability for the synthesis of key chiral intermediate of d -biotin and the substrate conversion could still keep 100% after 13 cycles continuously. Finally, optical pure (4 S , 5 R )-hemiester was obtained in 80.8% isolated yield and 99% purity in the gram preparative scale.
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危险品化学品经营许可证（带存储）