JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2022
·
IF 9.965
Sijia Liu,Xifei Zhou,Junxian Qin,Chaohai Wei,Yun Hu
Simultaneous redox reactions on photocatalysts make it possible to use wastewater for hydrogen production . The controlled synthesis of ultrasmall metal carbides effectively enhances the photocatalytic efficiency under this system. Here, we report a new type of cocatalyst in which a three-dimensional (3-D) nitrogen-doped carbon cage (NGC) of metal–organic framework derivatives encapsulates ultrasmall MoC nanoparticles (MoC@NGC), promoting simultaneous degradation of organic pollutants and hydrogen production by ZnIn 2 S 4 (ZIS). Characterization analyses showed that MoC accelerated the separation of the photogenerated carrier and effectively reduced the overpotential of hydrogen evolution , while NGC promoted the good dispersion of MoC particles and provided sufficient sites. The MoC@NGC/ZIS composite exhibited a high hydrogen (H 2 ) evolution rate of 1012 µmol g –1 h −1 , which exceed that of ZIS loaded with platinum. In the coupled system, where the electron donor was replaced with rhodamine B (RhB), the mechanism analysis showed that RhB and the as-generated intermediates consumed holes and facilitated hydrogen evolution. In addition, we designed a combined photocatalytic anoxic and oxic sequence process to achieve the recovery of hydrogen energy during the treatment of dye wastewater. This study provides a new way for cooperation between energy development and environmental protection.
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高影响因子
Crystals
·
2022
·Vol. 12
·期 2
·210
·
IF 2.67
Xingyuan Diao,Xiaonan Chen,Shihao Deng,Hongwei Bai
Stereocomplex-type polylactide (SC-PLA) created by alternate packing of poly(L-lactide) (PLLA) and poly(D-lactide) (PDLA) chains in a crystalline state has emerged as a growingly popular engineering bioplastic that possesses excellent hydrolytic stability and thermomechanical properties. However, it is extremely difficult to acquire high-performance SC-PLA products via melt-processing of high-molecular-weight PLLA/PDLA blends because both SC crystallites and homocrystallites (HCs) are competitively formed in the melt-crystallization. Herein, a facile yet powerful way was employed to boost SC formation by introducing trace amounts of some epoxy-functionalized small-molecule modifiers into the enantiomeric blends during reactive melt-blending. The results show that the SC formation is considerably enhanced with the in situ generation of multi-arm stereo-block PLA copolymers, based on the reaction between epoxy groups of the modifiers and hydroxyl end groups of PLAs. More impressively, it is intriguing to find that the introduction of only 0.5 wt% modifiers can induce exclusive SC formation in the blends upon isothermal and non-isothermal melt-crystallizations. The outstanding SC crystallizability might be attributed to the suppressing effect of such unique copolymers on the separation of the alternately arranged PLLA/PDLA chain segments in molten state as a compatibilizer. Furthermore, the generation of these copolymers does not result in a significant increase in melt viscosity of the blends. These findings suggest new opportunities for the high-throughput processing of SC-PLA materials into useful products.Keywords:polylactide;stereocomplex;crystallization;copolymer;compatibilization
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CHEMICAL ENGINEERING JOURNAL
·
2022
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IF 16.744
Bin Hu,An-shuai Cheng,Yang Li,Yao-bing Huang,Ji Liu,Bing Zhang,Kai Li,Li Zhao,Ti-peng Wang,Qiang Lu
Selective preparation of value-added products via fast pyrolysis is a sustainable way for biomass utilization. We developed a novel strategy, oxalic acid-assisted fast pyrolysis (OAFP) of cellulose, which achieved the selective production of a promising anhydrosugar, 1,4:3,6-dianhydro-α- d -glucopyranose (DGP), for the first time. Effects of temperature and the mass ratio of cellulose-to-oxalic acid (Cell/OA) in their solid mixture were explored. The yield of DGP could be up to 14.0 wt% (based on cellulose) at 400 °C and the Cell/OA mass ratio of 1:9 with a lab-scale setup, about 200 times higher than that from fast pyrolysis of pure cellulose. According to the experiments and density functional theory (DFT) calculations, it was OA rather than its pyrolysis vapors (mainly formic acid) that played the predominant role in the selective generation of DGP, where OA acted as a reactant and a catalyst-like material at the same time. The formation of DGP competed with that of levoglucosan (LG), the most typical product in the fast pyrolysis of pure cellulose, and a small amount of DGP came from the secondary conversion of LG. The esterification of free hydroxyl at C3 or C6 position with OA determined the selective generation of DGP by promoting the formation of the C1 = C2 bond and the 3,6-acetal ring, and the generation of the 1,6-acetal ring was inhibited correspondingly which led to the decrease of LG. In addition, the OAFP process also showed the potential for poly-generation of value-added chemicals, syngas, and char.
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MOLECULES
·
2022
·Vol. 27
·期 8
·2419
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IF 4.927
Xiao-Ling Wang,Wen-Zheng Zhao,Jia-Ze Fan,Le-Chen Jia,Ya-Nan Lu,Ling-Hui Zeng,Yuan-Yuan Lv,Xiao-Yi Sun
Tumor penetration and the accumulation of nanomedicines are crucial challenges in solid tumor therapy. By taking advantage of the MSC tumor-tropic property, we developed a mesenchymal stem cell (MSC)-based drug delivery system in which paclitaxel (PTX)-encapsulating hyaluronic acid-poly (D,L-lactide-co-glycolide) polymeric micelles (PTX/HA-PLGA micelles) were loaded for glioma therapy. The results indicated that CD44 overexpressed on the surface of both MSCs and tumor cells not only improved PTX/HA-PLGA micelle loading in MSCs, but also promoted the drug transfer between MSCs and adjacent cancer cells. It was hypothesized that CD44-mediated transcytosis played a crucial role and allowed deep glioma penetration depending on sequential intra–intercellular delivery via endocytosis–exocytosis. MSC-micelles were able to infiltrate from normal brain parenchyma towards contralateral tumors and led to the eradication of glioma. The survival of orthotopic glioma-bearing rats was significantly extended. In conclusion, the MSC-based delivery of HA-PLGA micelles is a potential strategy for tumor-targeting drug delivery.Keywords:mesenchymal stem cells;hyaluronic acid;poly (lactide-co-glycolide);orthotopic glioma;tumor penetration
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2022
·
IF 5.999
Yifeng Wang,Xi Luo,Xiaolong Sun,Jiahuan Hu,Qing Guo,Baoxing Shen,Yongqian Fu
In this study, we synthesized a novel biocatalyst by encapsulating lactate dehydrogenase (LDH) in the metal-organic framework ZIF-90 by one-pot embedding. It showed strong biological activity for efficient synthesis of D -phenyllactic acid ( D -PLA). The morphology and structure of LDH@ZIF-90 was systematically characterized by powder X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, confocal laser scanning microscopy (CLSM) and gas sorption . According to thermogravimetric analysis (TGA), the enzyme loading of the biocatalyst was 3 %. The Michaelis–Menten constant (K m ) and maximal reaction rate (V max ) of LDH@ZIF-90 were similar to those of free LDH, which proved that ZIF-90 had good biocompatibility to encapsulate LDH. At the same time, LDH@ZIF-90 exhibited enhanced tolerance to temperature, pH and organic solvents , and its reusability was greatly improved with 68 % of initial enzyme activity remaining after 7 rounds of recylcing. Overall, LDH encapsulated in ZIF-90 may be an economically competitive and environmentally friendly novel biocatalyst for the synthesis of D -PLA.
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ACS Sensors
·
2022
·
IF 9.618
Shuju Zhao,Xiujuan Qiao,Min Chen,Yanxin Li,Xin Wang,Zhenying Xu,Yumin Wu,Xiliang Luo
The susceptibility of peptides to proteolytic degradation in human serum significantly hindered the potential application of peptide-based antifouling biosensors for long-term assaying of clinical samples. Herein, a robust antifouling biosensor with enhanced stability was constructed based on peptides composed of d-amino acids (d-peptide) with prominent proteolytic resistance. The electrode was electropolymerized with poly(3,4-ehtylenedioxythiophene) and electrodeposited with Au nanoparticles (AuNPs), and the d-peptide was then immobilized onto the AuNPs, and a typical antibody specific for immunoglobulin M (IgM) was immobilized. Because of the effect of d-amino acids, the d-peptide-modified electrode surface showed prominent antifouling capability and high tolerance to enzymatic hydrolysis. Moreover, the d-peptide-modified electrode exhibited much stronger long-term stability, as well as antifouling ability in human serum than the electrode modified with normal peptides. The electrochemical biosensor exhibited a sensitive response to IgM linearly within the range of 100 pg mL–1 to 1.0 μg mL–1 and a very low detection limit down to 37 pg mL–1, and it was able to detect IgM in human serum with good accuracy. This work provided a new strategy to develop robust peptide-based biosensors to resist the proteolytic degradation for practical application in complex clinical samples.
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International Journal of Nanomedicine
·
2021
·
IF 6.4
Qiangqiang Wang,Xiaoke Sun,Xin Huang,Jianmei Huang,Muhammad Waqqas Hasan,RuoFeng Yan,Lixin Xu,Xiaokai Song,Xiangrui Li
Background Hepatocellular carcinoma-associated antigen 59 (HCA59) from excretory/secretory products of Haemonchus contortus is known to have the ability to modulate the functions of host cells. However, its immunogenicities using different nanoparticles adjuvants remain poorly understood. Purpose The study aimed to select an efficient nanoparticle antigen delivery system, which could enhance the immune responses of Haemonchus contortus HCA59 in mice. Methods Here, the immune responses induced by the recombinant protein of HCA59 (rHCA59) with poly-D,L-lactide-co-glycolide (PLGA) nanoparticles, Chitosan nanoparticles, mixture of PLGA and Chitosan nanoparticles (rHCA59-Chitosan-PLGA), and Freund’s complete adjuvant were observed, respectively, in mice. Cytokine and antibody levels induced by different groups were detected by ELISA assay. The effects of lymphocyte proliferations on different groups were examined using CCK-8 kit. Phenotypes of T cells and dendritic cells were analyzed by flow cytometry. Results On day 14 post vaccination, levels of IgM, IgG1, IgG2a, IFN-γ, IL-4, and IL-17 were significantly increased in the groups immunized with rHCA59 encapsulated with nanoparticles. After mice were vaccinated with rHCA59 loaded with Chitosan/PLGA nanoparticles, lymphocytes proliferated significantly. Additionally, the percentages of CD4 + T cells (CD3 + CD4 + ), CD8 + T cells (CD3 + CD8 + ), and dendritic cells (CD11c + CD83 + , CD11c + CD86 + ) were obviously up-regulated in the mice immunized with nanoparticles, especially in the rHCA59-Chitosan-PLGA antigen delivery system group. Conclusion The findings of this research demonstrated that rHCA59-Chitosan-PLGA antigen delivery system could induce higher immune responses in mice model and indicated that rHCA59 might be a good candidate molecule to develop nanovaccines against Haemonchus contortus in future study.
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ACS Sustainable Chemistry & Engineering
·
2021
·
IF 8.198
Kun Liu,Shengyue Tang,Wenyan Li,Wei Wen,Shan Ding,Mingxian Liu,Hong Li,Changren Zhou,Binghong Luo
To date, designing a perfect poly(d,l-lactide) (PDLLA) bone repair material still remains a major challenge. Inspired by the superior cell affinity, osteogenic activity, and mechanical properties of polysaccharide chitin whiskers, here, positively charged chitin whiskers (HCHW) and negatively charged maleated chitin whiskers (mCHW) are prepared for strengthening and biofunctionalizing the PDLLA membrane via a layer-by-layer (LBL) electrostatic self-assembly method. At a pH value of 4.3, the process of LBL electrostatic self-assembly between the HCHW and mCHW on the membranes is successfully performed, which can be directly proved by the cross-sectional morphology, light transmittance, surface composition, and topography of the membranes. Especially, polydopamine, as an intermediate layer, can greatly enhance the stability and adhesion of the first chitin whisker layer on the PDLLA substrate. Compared with the PDLLA membrane, the mechanical properties of self-assembly membranes are highly enhanced and controllable by adjusting the number of self-assembly whisker layers. Furthermore, in vitro biological evaluations indicate that the HCHW-assembled surface has a higher cell affinity than that of the mCHW-assembled one, which may be attributed to different protein adsorption. Meanwhile, interesting directional cell migration behavior is observed on the HCHW-assembled surface, which can be explained by the guiding effect of surface ordered topology and collective cell migration. These findings provide a new strategy for designing bioactive PDLLA composites with superior and tunable mechanical properties for bone regeneration materials.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2020
·Vol. 27
·期 11
·11990-12000
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IF 3.056
Cui Yanping,Thathsarani Nimesha,Peng Liang,Gao Ya,Lei Lidan,Zhou Zhengwei,Liang Lili,Shi Xiaoyan
Innovative paramagnetic one-dimensional (1-D) core-shell Fe-Ag@AgCl visible light-driven photocatalysts are synthesized through a template-assisted electrodeposition method trailed by FeCl 3 in-situ oxidation. The metallic nature of Fe-Ag@AgCl is confirmed through scanning electron microscopy (SEM) and crystal nature through X-ray diffraction (XRD). The controllable diameter of Fe-Ag is obtained through the selection of hollow size of the polycarbonate (PC) template. Electron impedance spectroscopy (EIS) confirms through the introduction of Fe to the Ag core that has prolonged the recombination of electron and hole. Escherichia coli ( E. coli ) are employed as the target bacteria to evaluate the photocatalytic disinfection performances. A total of 1.30 mg of Fe-Ag@AgCl is proved to be able to completely inactivate 10 7 CFU (colony forming units)/mL after 120 min of visible light irradiation. The transition electron microscopy (TEM) confirms the stability of the material after the photo reaction. As Fe-Ag@AgCl possesses magnetic properties, the material is recovered through the application of an external magnetic field. SEM images and results of 3D emission extraction matrix (EEM) depict that the bacteria cell death is caused by membrane permeability changes caused by the reduction of membrane associated proteins.
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Chemical and Biological Technologies in Agriculture
·
2026
·
IF 5.8
Tian Shu,Cheng Feng,Wu Pan,Jia Xuewei,Qu Lili,Xu Chunping
Background Microbial exopolysaccharides (EPS) are renewable biopolymers of increasing importance in the agri-food sector, and producing them from low-cost substrates offers a route to valorize agro-industrial resources. Aureobasidium pullulans is an industrially important fungus that secretes the α-glucan pullulan; however, the structure of the EPS it produces under lactose fermentation—a means of upgrading a low-value, dairy-derived carbon source—has not been clearly defined, which limits its rational use as an agri-food bioproduct. This study aimed to produce, structurally characterize, and preliminarily evaluate the physiological activity of this EPS. Results A homogeneous EPS was obtained by ethanol precipitation, Sevag deproteinization, and gel-filtration chromatography. Fourier-transform infrared spectroscopy indicated an α-configured, neutral polysaccharide, and gas chromatography identified glucose as the sole monosaccharide. Size-exclusion chromatography coupled with multi-angle laser light scattering showed a high-molecular-weight polymer. One- and two-dimensional NMR analyses established that the EPS is a linear pullulan-type α-D-glucan with the repeating unit [→6)-α-D-Glcp-(1→4)-α-D-Glcp-(1→4)-α-D-Glcp-(1→] n, in which two α-(1→4)-linked glucose residues alternate with an α-(1→6) linkage. In a high-fat-diet-induced obese rat model, dietary supplementation with the glucan attenuated body-weight gain, improved serum lipid profiles (TG, TC, HDL-C/TC), and lowered serum endotoxin and tumour necrosis factor-α, together with enhanced colonic short-chain fatty acid production, consistent with fermentation of the glucan by the gut microbiota. Conclusions Lactose fermentation by A. pullulans yields a structurally defined, linear pullulan-type α-D-glucan, demonstrating a route to convert a low-value dairy-derived substrate into a value-added biopolymer. Its prebiotic-associated metabolic effects indicate potential as a functional ingredient for the agri-food chain and warrant further mechanistic and safety studies. Graphical abstract
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Analytical Methods
·
2023
·Vol. 15
·期 44
·6009-6014
·
IF 3.1
Qin-Feng Zhang,Hua-Ming Xiao,Na An,Quan-Fei Zhu,Yu-Qi Feng
Vitamin D (VD) metabolites are involved in a variety of important metabolic processes and physiological effects in organisms. Profiling of VD metabolites favors a deep understanding of the physiological role of VD. However, VD metabolites are difficult to detect due to their high chemical structural rigidity, structural similarity, and low sensitivities under liquid chromatography-tandem mass spectrometry (LC-MS). Herein, we present a chemical derivatization assisted LC-MS/MS strategy for the detection of VDs, in which 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) is employed to derivatize the conjugated diene of VD metabolites and provides sensitizing reporters for MS detection. After PTAD derivatization, the sensitivities of seven VD metabolites increased by 24–276 folds, with the limits of detection ranging from 3 to 20 pg mL−1. Using this method, we achieved a sensitive and accurate quantification of 7 VD metabolites (vitamin D2, vitamin D3, 25-hydroxyvitamin D2, 25-hydroxyvitamin D3, 1,25-dihydroxyvitamin D2, 1,25-dihydroxyvitamin D3, and 1,24,25-trihydroxyvitamin D3) of the VD metabolic pathway in different trace biological samples, including human serum, mouse tissues (namely liver, kidney, lung, and spleen), and cells. We believe that the present method can provide a promising tool for an in-depth analysis of VD metabolism.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 12
·9757-9763
·
IF 3.201
Yalan Liu,Jiao Li,Gang Chang,Ruizhi Zhu,Hanping He,Xiuhua Zhang,Shengfu Wang
Electrochemical sensors have been highly developed and play an important role in the early diagnosis of different diseases. In this work, a simple and rapid electrochemical method was developed for the quantitative detection of a trinucleotide repeat (TNR) sequence d(CAG)n based on magnetic beads and screen-printed carbon electrodes. Their combination greatly simplified the operation process. The intensity of the electrochemical signal was linearly correlated against the concentration of the TNR sequence d(CAG)n in the range from 100 pM to 1 μM, with a limit of detection as low as 24 pM. Furthermore, the number of repeats of the TNR sequence d(CAG)n could be detected and also showed a good linear relationship. The proposed method provides a promising rapid and simple method for the detection of a CAG TNR sequence for point-of-care testing in clinical applications.
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危险品化学品经营许可证（带存储）