ACS Sensors
·
2025
·
IF 9.1
Xiaoyuan Wei,Qing-Ying Luo,Yeqing Li,Jing Yuan,Mengying Deng,Xinyu Liu,Peiluan Zhong,Haiqiao Ouyang,Yanfei Li,Jinlang Huang,Honghua Quan,Jun Chu,Xuefeng Yu,Wenhua Zhou,Zongwen Jin
Over recent years, the LUMinescent AntiBody Sensor (LUMABS) system, utilizing bioluminescence resonance energy transfer (BRET), has emerged as a highly effective method for antibody detection. This system incorporates NanoLuc (Nluc) as the donor and fluorescent protein (FP) as the acceptor. However, the limited Stokes shift of FP poses a challenge, as it leads to significant spectral cross-talk between the excitation and emission spectra. This issue complicates the implementation of multiplexed detection. To address this challenge, we present an innovative enhancement to the LUMABS sensor with quantum dots (QDs) as the acceptor instead of FP. The use of QDs offers several advantages over those of traditional FP-based sensors. The biotin–avidin system facilitates the flexible interchangeability of QDs, allowing for a more convenient multicolor sensor construct. The new QD-LUMABS system overcomes the limitations of spectral cross-talk and provides better spectral separation. This breakthrough enables the successful implementation of multiplexed detection for multiple targets simultaneously. Results demonstrated that the wavelength-tunable QD-LUMABS sensors achieved picomolar-level detection limits for antibodies and that this sensor-construction strategy was generally applicable among various epitopes and their antibodies. Furthermore, this sensor displayed excellent duplexing capabilities. These features underscore its potential for future clinical disease diagnosis applications.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Nana Li,Ying Han,Wanqiu Huang,Liangqiang Chen,Mengdi Hao,Huabin Tu,Hao Shen,Shaoning Yu,Fan Yang
In natural world, sophisticated cascade reactions occur in compartmentalized organelles. However, simulating cascade processes in living systems remains problematic, and the drawbacks of enzymes, including dissatisfactory stability, reusability, and sensitivity in extreme microenvironments, have hampered further applications. Here, we report a confined multi-enzyme system in which Mn Fe metal-organic framework (MFM) is applied to encapsulate enzymes through shell-by-shell epitaxial-overgrowth in diverse compartments. MFM possessed excellent peroxidase-mimicking activity and biocompatibility, enabling it to serve not only as a reliable carrier for multiple enzymes, but also as a high-performance nanozyme in cascades. Compared with free enzymes, this system exhibited significantly improved bioactivity and environmental tolerance. On this basis, the confined multi-enzyme system was applied for selective α-amylase analysis in complicated fermentation specimens with a broad linear range (5–500 U·L −1 ) and low detection limit (2.14 U·L −1 ). This work sheds new light on the construction of efficient biocatalytic cascades to accelerate applications in food manufacturing.
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FISH & SHELLFISH IMMUNOLOGY
·
2024
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IF 4.1
Huaxing Su,Yitong Zhang,Zhongwei He,Yucong Yang,Yuqin Ren,Wei Cao,Yufeng Liu,Jiangong Ren,Yufen Wang,Guixing Wang,Chunguang Gong,Jilun Hou
Ube3a is a member of the E3 ubiquitin ligase HECTc family, and its role has been established in neurodevelopmental disorders. However, studies on its role in Japanese flounder are scarce. Thus, in this study, the ube3a of Japanese flounder was cloned, and its role in conferring resistance against Chinook salmon bafnivirus (CSBV) was analyzed. Japanese flounder ube3a encoded a protein containing 834 amino acids. Interestingly, its homology with the Atlantic halibut was determined to be 94%. In addition, there were differential expressions of ube3a in different tissues of Japanese flounder, with the highest expression level observed in the fin, followed by the gills and skin ( P ≤ 0.05). Subcellular localization analysis revealed that Ube3a is a cytoplasmic protein. We established an in vitro CSBV infection model using Japanese flounder gill cell line (FG). After ube3a overexpression, the viral load was significantly lower than that of the control group ( P ≤ 0.05). Contrastingly, after incubation of FG cells with an E3 ubiquitin ligase inhibitor, the viral load was significantly higher than in the control group ( P ≤ 0.01). Then, the expression levels of nf-κb , traf3 , and tnf-α after incubation with an E3 ubiquitin ligase inhibitor were examined. The results demonstrated that ube3a may exerted a significant antiviral effect in Japanese flounder via the ubiquitination pathway.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xing Cheng,Yue Tang,Xiaohuan Huang,Linghui Huang,Han Tao,Yuangen Wu
Artificial enzymes have attracted much attention in food analysis because of their simple preparation, low cost, and strong stability. However, the most used artificial enzymes are based on oxidoreductases such as oxidase, peroxidase, catalase, and laccase. There are few reports on hydrolases like phosphatase for food analysis. Herein, a novel green zirconium-glutamic acid (Zr(IV)-Glu) complex was prepared using Zr ions and glutamic acid. The Zr(IV)-Glu complex was first found to possess excellent phosphatase-like activity, which was used to establish a fluorescent sensor for detecting phytic acid (PA). The Zr(IV)-Glu complex can catalyze the hydrolysis of 4-methylumbellione phosphate disodium salt (4-MUP) to produce blue fluorescent products with fluorescence emission peaks at 448 nm. PA with six phosphate groups can attach on the surface of Zr(IV)-Glu to form Zr-O-P bond and inhibit the catalytic activity of Zr(IV)-Glu, leading to the decrease of fluorescence in the system. The change of fluorescent intensity at 448 nm was positively correlated with the PA content, so that a fluorescent sensor using the phosphatase-like activity of Zr(IV)-Glu was proposed to detect PA. The established sensor has high sensitivity, low detection limit (LOD) of PA to 58 μg·L -1 , strong anti-interference ability, and excellent selectivity. This work can provide a new method for PA detection in food and further expand the application of phosphatase mimics.
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HARMFUL ALGAE
·
2025
·
IF 4.5
Ying Wei,Yunni Gao,Jing Wu,Jing Dong,Jingxiao Zhang,Xiaofei Gao,Huatao Yuan,Xuejun Li,Michele A Burford
Targeting and selectivity are essential for assessing the effectiveness of ecofriendly algaecides in controlling toxic cyanobacterial blooms. The dose- and time-dependent effects of hydrogen peroxide (H 2 O 2 ), pyrogallol and their sustained-release microspheres (HRM, PRM) on proportion of MC-producing cell numbers and genotypes of the co-existing MC- and non-MC-producing Microcystis , as well as microcystins (MCs) concentrations were investigated in the present study. Two MC-producing and two non-MC-producing Microcystis strains were used in mono- and co-culture test systems. The findings revealed that the MC-producing Microcystis strains were more sensitive to both forms of H 2 O 2 and pyrogallol in comparison with the non-MC-producing strains. Inhibition of all Microcystis strains by H 2 O 2 and pyrogallol was found to be dose- and time-dependent, with H 2 O 2 exhibiting stronger effects. Additionally, both pure and granular forms of H 2 O 2 and pyrogallol reduced the relative abundance of MC-producing genotypes in co-culture conditions, along with total MC concentrations. The HRM and PRM demonstrated more potent and prolonged effects than their pure forms, indicating they are optimal for targeted reduction of Microcystis bloom toxicity. The release rate of MCs into the extracellular environment of Microcystis may exceed their degradation rate during H 2 O 2 and pyrogallol treatments. It resulted in elevated extracellular MC levels after treatment. Therefore, ongoing monitoring of environmental MCs is necessary following the inhibition of Microcystis population growth by algaecides.
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Materials
·
2025
·Vol. 18
·期 4
·796
·
IF 3.2
Lujun Niu,Qiren Sun,Shijia Wei,Dixiang Gong,Enhui Wang,Yan Chen,Lu Xia,Xingyu Liu,Langping Tu,Long Shao,Hongfei Li,Jing Zuo
Boosting the accuracy and speed of cancer detection is highly desirous in tumor detection, and sensors capable of detecting carcinoembryonic antigen (CEA) have great application prospects in this field. A highly sensitive sensor is constructed based on the fluorescence resonance energy transfer (FRET) with heavily rare-earth-doped upconversion nanoparticles (UCNPs) as energy donors and polydopamine nanoparticles (PDA NPs) as energy acceptors. This sensor detects the fluctuations in CEA molecules via luminescence quenching and recovery resulting from a competitive binding assay between CEA and PDA NPs. The high-level-doped design of UCNPs (i.e., NaYF4@NaYbF4:1%Tm@NaYF4) is beneficial, providing upconversion luminescence intensity that is more than 10 times higher than that of the conventional low-level-doped UCNPs (i.e., NaYF4@NaYF4:20%Yb, 0.2%Tm@NaYF4). The sensor exhibits impressive sensitivity. Specifically, in diluted fetal bovine serum, the detection limit reaches 0.013 ng/mL in the range of 0–1.5 ng/mL (S/N = 3), while the detection limit is 1.38 ng/mL in the range of 1.5–250 ng/mL (S/N = 3). This method has great potential for future applications in the rapid and early diagnosis and treatment of cancer.
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CHEMICAL ENGINEERING JOURNAL
·
2024
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IF 13.3
Yong Li,Xi Liu,Shuangfei Xiang,Shujun Zhao,Feiya Fu,Hongyan Diao,Xiangdong Liu
Janus fabrics with moisture management abilities have garnered significant attention for enhancing human physiological and thermal comfort. However, excessive sweating can lead to fabric flooding and diminished wicking capabilities once capillary channels within are saturated, thereby reducing wearer comfort and encouraging bacterial growth. Inspired by the oxidation and reduction behaviors of disulfide bonds in the perm process, we creatively anchor sericin and protamine onto to a single surface of hydrophobic polyester (PET) fabrics using a mist technique, enabling efficient water transport and antibacterial properties. Through fabrication process, sericin is covalently linked to the fabric, resulting in a wetting gradient ranging from hydrophilic to hydrophobic. Consequently, the Janus PET fabric (HAJP) attains diode-like unidirectional water transport properties, exhibiting remarkable unidirectional transport capability (with an R-value of 1080 %). Furthermore, protamine electrostatically adsorbed endows HAJP fabric with excellent antibacterial properties, effectively inhibiting growth of both Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) by 100 %. Even after 50 wash cycles and 600 rubbing cycles, the residual germicidal effect remains over 94 %. Additionally, benefiting from unidirectional water transport and rapid surface evaporation, HAJP fabric can lower human body temperature by 3–4 °C compared to conventional textiles like original PET and cotton fabric, implying potential utility in hot weather conditions. This work provides new insights and a promising research direction for the manufacture of advanced moisture management textiles with high antibacterial performance.
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JOURNAL OF MOLECULAR NEUROSCIENCE
·
2024
·Vol. 74
·期 1
·1-20
·
IF 2.8
Fan Yubao,Huang Shanshan,Li Fugui,Zhang Xiyu,Huang Xueying,Li Weiqiang,Zeng Jixiao,Wang Weijia,Liu Jia
The sympathetic nervous system (SNS) is a crucial branch of the autonomic nervous system (ANS) that is responsible for regulating visceral function and various physiological processes. Dysfunction of the SNS can lead to various diseases, such as hypertension and metabolic disorders. However, obtaining sympathetic neurons from human tissues for research is challenging. The current research aimed at recapitulating the process of human sympathetic neuron development and achieved the successful establishment of a stepwise, highly efficient in vitro differentiation protocol. This protocol facilitated the generation of functional and mature sympathetic neurons from human pluripotent stem cells (hPSCs) using a chemical-defined induction medium. Initially, each differentiation stage was refined to derive sympathoadrenal progenitors (SAPs) from hPSCs through neural epithelial cells (NECs) and trunk neural crest stem cells (NCSCs). hPSC-derived SAPs could be expanded in vitro for at least 12 passages while maintaining the expression of SAP-specific transcription factors and neuronal differentiation potency. SAPs readily generated functional sympathetic neurons (SymNs) when cultured in the neuronal maturation medium for 3–4 weeks. These SymNs expressed sympathetic markers, exhibited electrophysiological properties, and secreted sympathetic neurotransmitters. More importantly, we further demonstrated that hPSC-derived SymNs can efficiently regulate the adipogenesis of human adipose–derived stem cells (ADSCs) and lipid metabolism in vitro. In conclusion, our study provided a simple and robust protocol for generating functional sympathetic neurons from hPSCs, which may be an invaluable tool in unraveling the mechanisms of SNS-related diseases.
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Microorganisms
·
2025
·Vol. 13
·期 4
·798
·
IF 4.2
Xiaoyu Cao,Yu Zhang,Qianqian Xu,Hai Yan
Hyperuricemia is a metabolic disorder owing to the underexcretion and/or overproduction of uric acid (UA). Recent studies have shown that probiotics have many potential applications as functional foods to ameliorate hyperuricemia. In this study, we have successfully isolatedBacillus paranthracisYC03 with urate-lowering potential from Jiangshui. The in vitro results indicated that YC03 exhibited strong biodegradation capacities toward UA and its precursors (inosine and guanosine). Meanwhile, the cell-free extracts of YC03 were also found to catalyze and remove inosine and guanosine. To further explore the application potential of this strain in developing functional foods, we evaluated its probiotic properties through in vitro assays and whole genome analysis.B.paranthracisYC03 has excellent abilities, with tolerance to acid and bile salt and good adhesion. In addition, hemolytic assays, along with antibiotic resistance and biogenic amine production tests, have also preliminarily confirmed the safety of using YC03 in food. We have also annotated the key enzyme genes, includingauaG,hpt,rih,punAanddeoD, which are involved in the biodegradation of UA and nucleosides. The results of nucleoside biodegradation product detection will be valuable for exploring the metabolic pathway for biodegrading nucleosides with YC03. These findings provide meaningful insights for the development of functional foods to improve hyperuricemia usingB.paranthracisYC03.
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Theranostics
·
2024
·
IF 12.4
Jing Ye,Xinyuan Bi,Shiyu Deng,Xianghui Wang,Ze Liu,Qian Suo,Jiao Wu,Haoran Chen,Yong Wang,Kun Qian,Rubing Shi,Jing Zhao,Guo-Yuan Yang,Jian Ye,Yaohui Tang
Rationale: Stroke induces metabolic changes in the body, and metabolites have become potential biomarkers for stroke. However, the specific metabolites involved in stroke and the mechanisms underlying brain injury during stroke remain unclear. Methods: Surface-enhanced Raman spectroscopy (SERS) and liquid chromatography-mass spectrometry (LC‒MS) analysis of clinical serum samples from 69 controls and 51 ischemic stroke patients who underwent reperfusion within 24 hours were performed to identify differentially abundant metabolites. Mice were subjected to transient middle cerebral artery occlusion (tMCAO) and then intravenously injected with hypoxanthine. The infarct area was evaluated via tetrazolium chloride (TTC) staining, and behavior tests were conducted. Blood-brain barrier (BBB) leakage was assessed by Evans blue and IgG staining. Human blood vessel organoids were used to investigate the mechanism of hypoxanthine-induced pyroptosis of endothelial cells. Results: SERS and LC‒MS revealed the metabolic profiles of serum from stroke patients and controls with high sensitivity, speed and accuracy. Hypoxanthine levels were significantly elevated in the acute stage of ischemic stroke in both patients and mice (p < 0.001 after Bonferroni correction). In addition, increasing hypoxanthine increased the infarct area and aggravated BBB leakage and neurobehavioral deficits in mice after ischemic stroke. Further mechanistic studies using endothelial cells, human blood vessel organoids, and stroke mice demonstrated that hypoxanthine-mediated gasdermin E (GSDME)-dependent pyroptosis of endothelial cells occurs through intracellular Ca 2+ overload. Conclusion: Our study identified hypoxanthine as an important metabolite that induces vascular injury and BBB disruption in stroke through triggering GSDME-dependent pyroptosis of endothelial cells.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Linwei Yu,Xi Li,Tianyi Shi,Ning Li,Donge Zhang,Xikai Liu,Yushuo Xiao,Xinran Liu,Robert B. Petersen,Weikang Xue,Yanxun V. Yu,De-sheng Hu,Li Xu,Hong Chen,Ling Zheng,Kun Huang,Anlin Peng
Parkinson's disease (PD), a neurodegenerative disorder without cure, is characterized by the pathological aggregation of α-synuclein (α-Syn) in Lewy bodies. Classic deposition pathway and condensation pathway contribute to α-Syn aggregation, and liquid-liquid phase separation is the driving force for condensate formation, which subsequently undergo liquid-solid phase separation to form toxic fibrils. Traditional Chinese Medicine (TCM) has a long history in treating neurodegenerative disease; herein, we identified chemicals from herbs that inhibit α-Syn aggregation. We screened commonly prescribed TCMs for PD from the CNKI database and registered patents, 13 chemicals were identified in the TCMSP databases as candidate inhibitors, among which three phenols, forsythoside B (FTSB), echinacoside (ECH), and 4-hydroxyindole (C4-OH) efficiently inhibit α-Syn aggregation. Moreover, FTSB and ECH increase α-Syn fluidity within condensates, inhibit α-Syn transition into amyloid fibrils and reduce fibril-induced toxicity in SH-SY5Y cells. Importantly, they disaggregated preformed α-Syn amyloid fibrils. Notably, in an α-Syn overexpressing NL5901 C. elegans PD model, either FTSB or ECH treatment significantly extended the lifespan and improved the PD-like movement disorders, both in the preventive and therapeutic treatment approaches, by reducing toxic α-Syn inclusion formation and improving the fluidity of α-Syn. Together, we offer new therapeutic candidates targeting phase separation-associated aggregation for PD.
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CELLULAR SIGNALLING
·
2024
·
IF 4.4
Xin Chen,Liming Yu,Shan Meng,Jikai Zhao,Xinyi Huang,Zhishang Wang,Zijun Zhou,Yuting Huang,Tao Hong,Jinfeng Duan,Tong Su,Zijun Cao,Yanbang Chi,Tao Huang,Huishan Wang
Inflammation and infiltration of immune cells are intricately linked to the pathogenesis of atrial fibrillation (AF). Triggering receptor expressed on myeloid cells-1 (TREM-1), an enhancer of inflammation, is implicated in various cardiovascular disorders. However, the precise role and potential mechanisms of TREM-1 in the development of AF remain ambiguous. Atrial samples from patients with AF were used to assess the expression levels of TREM-1. An angiotensin II (Ang II)-induced AF mouse model was established to assess the functionality of TREM-1. Cardiac function and AF inducibility were assessed through echocardiography, programmed transvenous cardiac pacing, and atrial electrophysiological mapping. Peripheral blood and atrial inflammatory cells were assessed using flow cytometry. Using histology, bulk RNA sequencing, biochemical analyses, and cell cultures, the mechanistic role of TREM-1 in AF was elucidated. TREM-1 expression was upregulated and co-localized with macrophages in the atria of patients with AF. Pharmacological inhibition of TREM-1 decreased Ang II-induced atrial enlargement and electrical remodeling. TREM-1 inhibition also ameliorated Ang II-induced NLRP3 inflammasome activation, inflammatory factor release, atrial fibrosis, and macrophage infiltration. Transcriptomic analysis revealed that TREM-1 modulates Ang II-induced inflammation through the PI3K/AKT/FoxO3a signaling pathway. In vitro studies further supported these findings, demonstrating that TREM-1 activation exacerbates Ang II-induced inflammation, while overexpression of FoxO3a counteracts this effect. This study discovered the critical role of TREM-1 in the pathogenesis of AF and its underlying molecular mechanisms. Inhibition of TREM-1 provides a new therapeutic strategy for the treatment of AF.
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