MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 7
·1-7
·
IF 5.3
Wang Shirong,Wei Zaiwa,Li Liangxian,Luo Yu,Huang Zhimei,Yang Xing,Tang Yafang
CRISPR-Cas12a with robust trans-cleavage activity were employed to mitigate background fluorescence signal, achieving sensitive detection of miRNA-21. The activation of trans-cleavage activity of Cas12a was achieved by utilizing cDNA as a trigger. Upon the presence of target miRNA-21, cDNA hybridizes with it forming a DNA/RNA double-stranded structure. Exonuclease III (ExoIII) facilitates the degradation of cDNA, releasing the target for subsequent cycles. Due to cDNA degradation, the trans-cleavage activity of Cas12a remains unactivated and does not disrupt the synthesis template of copper nanoparticles. Addition of Cu 2+ and AA leads to the formation of highly fluorescent copper nanoparticles. Conversely, in absence of miRNA-21, intact cDNA activates trans-cleavage activity of Cas12a, resulting in degradation of the synthesis template and failure in synthesizing fluorescent copper nanoparticles. This method exhibits excellent selectivity with a low limit of detection (LOD) at 5 pM. Furthermore, we successfully applied this approach to determine miRNA-21 in cell lysates and human serum samples, providing a new approach for sensitive determination of biomarkers in biochemical research and disease diagnosis. Graphical Abstract
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高影响因子
RESEARCH ON CHEMICAL INTERMEDIATES
·
2022
·Vol. 48
·期 3
·1181-1198
·
IF 3.134
Zhang Zhongze,Liu Zonghui,Gu Zhiyuan,Wen Zhe,Xue Bing
The catalytic transfer hydrogenation (CTH) reaction of ethyl levulinate (EL) with alcohol to γ-valerolactone (GVL) has been investigated over a series of Zr-based catalysts, including ZrO 2 , ZrP, Zrβ, ZrP/Hβ and ZrO 2 /HZSM-5. It is found that the simplest ZrO 2 was the most efficient for GVL production, giving an EL conversion as high as 97% and GVL selectivity of 91% after 7 h at 170 °C using 2-propanol as the hydrogen donor and solvent. The effects of reaction temperature, time and solvent on catalytic performance were investigated. Moreover, the kinetic behavior of CTH reaction of EL was also studied over ZrO 2 catalyst. The as-prepared ZrO 2 gives an Ea value of 32.9 kJ/mol, which is much lower than other catalysts in the literatures. Furthermore, those catalysts have been, respectively, characterized by XRD, IR, BET and NH 3 -TPD techniques to reveal the physical properties and structures of these materials. Correlating the catalyst performance with its physical and chemical properties uncovers that the higher pore diameter, lower total acidity and higher Lewis acid sites of catalyst surface would be the key to the catalyst performance. Graphical abstract
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JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 27
·12886-12893
·
IF 4.682
Ullah Wajid,Aziz Tariq,Ali Amjad,Khan Zaheen Ullah,Das Sandeep Kumar,Iqbal Mudassir,Ullah Roh,Wazir Nasrullah,Khan Farman Ullah,Raheel Muhammad
Solar photo-catalysis is very essential for both harvesting high-density solar energy, and environmental sustainability. A high surface area carbon nitride (HSACN), a photo-catalyst is synthesized by simple re-crystallization and calcination methods. The decrease in the size of urea crystals is ascribed to the use of different solvents and direct calcination that resulted in the formation of carbon nitride. The re-crystallization suggests that the defect-rich carbon nitride created nitrogen vacancies, which accelerated the photocatalytic activity of dyes’ degradation. The synthesized materials were characterized by X-ray powder diffraction, Field emission scanning electron microscopy, and transmission electron microscopy for structural and morphology evaluation. The corresponding high surface area carbon nitride demonstrated higher photocatalytic-dye-degradation efficiency under UV light irradiation, and higher adsorption capacity compared to graphitic carbon nitride, which is obtained without re-crystallization protocol. An increase in the surface area of HSACN has been observed due to structural disturbance caused by the growth of tiny urea crystals in carbon nitride. The nitrogen vacancies incorporation improved the absorption of extended visible light. The enhancement in photocatalytic activities are corresponding to the improved charge carrier photoexcitation, separation, and generated abundant reaction sites.
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RARE METALS
·
2021
·Vol. 41
·期 2
·630-638
·
IF 4.003
Qiu Jia-Jun,Yang Ting-Ting,Li Yi-Fan,Qian Wen-Hao,Liu Xuan-Yong
Although titanium and its alloys are extensively used in orthopedics and dentistry fields, implant failures still happen because of implant-associated infections. Herein, Au@Ag@Pt core–shell nanorods with noble metal combination were fabricated and assembled on medical titanium surface and the antibacterial activity and biocompatibility were investigated. The results showed that antibacterial rates of Ti–Au@Ag@Pt against S. epidermidis and P. aeruginosa were 89.7% and 92.7%, respectively. Besides, Ti–Au@Ag@Pt showed no obvious cell toxicity with MC3T3-E1 cells grew well on the sample surface. It was discovered that the Pt shell layer on Ti–Au@Ag@Pt slowed down the Ag ion release rate which endowed medical titanium surface with both antibacterial activity and good biocompatibility. Graphical abstract
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MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 10
·1-10
·
IF 5.833
Cheng Sijie,Zhang Junqiu,Liu Yaoming,Wang Yingte,Xiao Yanteng,Zhang Yong
Water soluble N, S-doped carbon dots (N, S-CDs) with orange emission were synthesized from basic fuchsin and sulfosalicylic acid by the typical hydrothermal route. Based on the inner filter effect (IFE), the prepared N, S-CDs can be innovatively developed as an effective “signal-off” multifunctional sensing platform for sensitive determination of tetracycline antibiotics (for example, chlortetracycline (CTC)) and quercetin. The proposed sensor was utilized to realize the determination of CTC in water and milk samples and quercetin in beer sample (λex = 375 nm, λem = 605 nm) with satisfactory recoveries and relative standard deviations (RSD). The linear range and detection limit (LOD) of CTC is 1.24–165 μM and 32.36 nM, respectively. For quercetin, the linear ranges are 0.98–34 μM and 34–165 μΜ, and the LOD is 6.87 nM (3σ/m). By virtue of the good biocompatibility and long-wavelength emission, N, S-CDs were also used in the imaging of oocystis cells and yeast cells, which demonstrated promising applicability for bio-imaging and sensing. Graphical abstract
In this paper, N, S-doped carbon dots (N, S-CDs) with orange emission (λem = 605 nm) were synthesized from basic fuchsin and sulfosalicylic acid. Based on the inner filter effect (IFE), the prepared N, S-CDs can be innovatively developed as an effective “signal-off” multifunctional sensing platform for the sensing of tetracycline antibiotics (for example: chlortetracycline (CTC)) and quercetin. The sensor has been successfully applied to the determination of CTC in water and milk samples and quercetin in beer sample (λex = 375 nm, λem = 605 nm). The linear range and detection limit (LOD) of CTC is 1.24-165 μM and 32.36 nM respectively. For quercetin, the linear ranges are 0.98-34 μM and 34-165 μΜ, and the LOD is 6.87 nM (3σ/m). In addition, due to the characteristics of good biocompatibility and long-wavelength emission, the N, S-CDs were also used in the imaging of oocystis cells and yeast cells, which demonstrated promising applicability for bioimaging and sensing.
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高影响因子
CATALYSIS LETTERS
·
2022
·Vol. 153
·期 4
·1170-1179
·
IF 2.936
Luo Mingsheng,Li Hong,Song Huanqiao,Shao Changke,Wang Aimei
The alkylation of phenol with methanol was studied in vapor phase over 5 wt% cerium supported on HZSM-5 catalyst prepared by three different methods, such as impregnation, ball mixing and solid-state ion-exchange. The catalytic activities are tested in a fixed-bed reactor, and the physicochemical properties of the catalyst are characterized with X-ray powder diffraction, scanning electron microscope, nitrogen physical sorption, Fourier Transform Infrared Spectrometer, Infrared spectra of pyridine adsorption, X-ray photoelectron spectroscopy and temperature-programmed desorption of ammonia. It was found that the dispersion form of cerium was greatly affected by the preparation method, and difference in acidity affects catalytic performance in the alkylation reaction of phenol and methanol. Ball milling method generated greater amount of strong Brønsted acidic sites and lower specific surface area than other catalysts, which led to higher carbon deposition rate and lower catalyst activity. The catalyst prepared by impregnation method yields higher Lewis acid concentration (2.09 μmol/g) and more chemisorbed oxygen due to the increased Ce 3+ concentration in the lattice, resulted in an improved o-cresol selectivity of 48%. Furthermore, the catalyst prepared by solid-phase ion-exchange method produces a catalyst with appropriate B/L ratio of 60.6 and large specific surface area of 389.0 m 2 /g, which yields the highest phenol conversion of 23%. Graphical Abstract
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高影响因子
BMC Complementary Medicine and Therapies
·
2026
·
IF 4.4
Zou Xian-Liang,Deng Gang,Wu Jian-Hua,Zhang Ting,Wang Yong-Li,Chou Gui-Xin
Background Rehmanniae Radix (RR), obtained from Rehmannia glutinosa , is widely used in traditional Chinese medicine. Phenylethanoid glycosides are relatively stable during processing and represent important bioactive constituents of Rehmanniae Radix Praeparata (RRP). This study aimed to establish an integrated analytical strategy combining ultra-performance liquid chromatography–electrospray ionization mass spectrometry (UPLC-ESI-MS), quantitative analysis of multi-components with a single marker (QAMS), and high-performance liquid chromatography (HPLC) fingerprinting for comprehensive quality evaluation of RRP. Methods UPLC-ESI-MS was used to identify the major constituents of RRP. Seven phenylethanoid glycosides were quantitatively determined by a validated UPLC-photodiode array (UPLC-PDA) method using QAMS with acteoside as the internal reference. Relative correction factors (RCFs) obtained by the average-value and linear regression methods were compared with those of the external standard method (ESM). HPLC fingerprint analysis was performed to evaluate batch-to-batch consistency. Results Eight compounds were identified in RRP, and seven phenylethanoid glycosides were quantitatively determined using the QAMS method with acteoside as the internal reference. The method showed excellent linearity (r² ≥ 0.9994), good precision, stability, and recovery (98.62%–102.64%), and the QAMS results were in good agreement with those obtained by ESM. HPLC fingerprint analysis of 17 batches of RRP showed similarity values ranging from 0.914 to 0.986, indicating good batch-to-batch consistency. Conclusion The proposed UPLC-PDA- and QAMS-based method, combined with HPLC fingerprint analysis, provides a practical, reliable, and cost-effective strategy for quality evaluation of RR and RRP.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2023
·Vol. 21
·期 1
·1-23
·
IF 10.2
Guo Rongkang,Wu Chaohan,Liu Fan,Dong Tianhua,Zhang Tao
Objective This study aims to investigate the mechanism by which biomimetic composite hydrogels loaded with bone marrow mesenchymal stem cells (BMSCs) derived microRNA-19b-3p/WWP1 axis through extracellular vesicles (EVs) affect the new bone formation in rat bone defects. Methods First, synthesize the bionic composite hydrogel Gel-OCS/MBGN. Characterize it through field-emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), and FTIR. Then, conduct performance tests such as rheology, dynamic mechanical analysis, in vitro mineralization, and degradation. Rat BMSCs were selected for in vitro cell experiments, and EVs derived from BMSCs were obtained by differential centrifugation. The EVs were loaded onto Gel-OCS/MBGN to obtain Gel-OCS/MBGN@EVs hydrogel. Cell viability and proliferation were detected by live/dead cell staining and CCK-8 assay, respectively. ALP and ARS staining was used to evaluate the osteogenic differentiation of BMSCs. Differential gene expression analysis of osteogenic differentiation was performed using high-throughput sequencing. TargetScan database predicted the binding site between miR-19b-3p and WWP1, and a dual-luciferase reporter assay was performed to confirm the targeting binding site. A rat bone defect model was established, and new bone formation was evaluated by Micro-CT, H&E staining, and Masson's trichrome staining. Immunofluorescence staining and immunohistochemistry were used to detect the expression levels of osteogenic-related factors in rat BMSCs. RT-qPCR and Western blot were used to detect the expression levels of genes and proteins in tissues and cells. Result Gel-OCS/MBGN was successfully constructed and loaded with EVs, resulting in Gel-OCS/MBGN@EVs. The in vitro drug release experiment results show that Gel-OCS/MBGN could sustainably release EVs. Further experiments have shown that Gel-OCS/MBGN@EVs could significantly promote the differentiation of BMSCs into osteoblasts. Experiments have shown that WWP1 is a key factor in osteogenic differentiation and is regulated by miR-19b-3p. EVs promote osteogenic differentiation by suppressing WWP1 expression through the transmission of miR-19b-3p. In vivo animal experiments have demonstrated that Gel-OCS/MBGN@EVs significantly promote bone repair in rats with bone defects by regulating the miR-19b-3p/WWP1 signaling axis. Conclusion Functional Gel-OCS/MBGN@EVs were obtained by constructing Gel-OCS/MBGN and loading EVs onto it. EVs could deliver miR-19b-3p to BMSCs, inhibit the expression of WWP1, and promote the osteogenic differentiation of BMSCs, ultimately promoting bone regeneration in rats with bone defects. Graphical Abstract
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高影响因子
Nature Protocols
·
2023
·
IF 14.8
Liu Renmei,Yao Jing,Zhou Siyu,Yang Jing,Zhang Yaqiang,Yang Xiaoyan,Li Leshi,Zhang Yunbin,Zhuang Yingping,Yang Yi,Chen Xianjun
RNA molecules perform various crucial roles in diverse cellular processes, from translating genetic information to decoding the genome, regulating gene expression and catalyzing chemical reactions. RNA-binding proteins (RBPs) play an essential role in regulating the diverse behaviors and functions of RNA in live cells, but techniques for the spatiotemporal control of RBP activities and RNA functions are rarely reported yet highly desirable. We recently reported the development of LicV, a synthetic photoswitchable RBP that can bind to a specific RNA sequence in response to blue light irradiation. LicV has been used successfully for the optogenetic control of RNA localization, splicing, translation and stability, as well as for the photoswitchable regulation of transcription and genomic locus labeling. Compared to classical genetic or pharmacologic perturbations, LicV-based light-switchable effectors have the advantages of large dynamic range between dark and light conditions and submicron and millisecond spatiotemporal resolutions. In this protocol, we provide an easy, efficient and generalizable strategy for engineering photoswitchable RBPs for the spatiotemporal control of RNA metabolism. We also provide a detailed protocol for the conversion of a CRISPR–Cas system to optogenetic control. The protocols typically take 2–3 d, including transfection and results analysis. Most of this protocol is applicable to the development of novel LicV-based photoswitchable effectors for the optogenetic control of other RNA metabolisms and CRISPR–Cas functions.
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高影响因子
JOURNAL OF IRON AND STEEL RESEARCH INTERNATIONAL
·
2023
·
IF 2.5
Guo Jing,Wang Hai-jing,Liu Shuang-juan,Zhang Peng,Tang Jing-yu,Zhang Wei-yang,Guo Han-jie,Wang Peng-cheng,Meng Cai
A room-temperature electrodeposition method with an organic electrolyte was developed to fabricate a HNO 3 -pretreated graphene paper Cu (GP′–Cu) composite. To improve the interfacial bonding of GP′–Cu composite, magnetron sputtering technology was used to create a “sandwich” structural gradient GP′–Cu composite. The selection of the intermediate transition layer metal was based on two-dimensional disregistry. Scanning electron microscopy, X-ray photoelectron spectroscopy, and other analytical methods confirmed that the addition of an intermediate transition metal (Cr, Ni) layer reduced the gap distance and enhanced the interfacial bonding of the GP′ and Cu deposited layers. The GP′–Ni–Cu composite exhibited the largest increase in tensile strength and conductivity. In addition, it had the highest thermal diffusivity and elongation at break among the GP′–Cu, GP′–Cr–Cu and GP′–Ni–Cu composites.
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高影响因子
Nature Communications
·
2026
·
IF 18.1
Hu Yingjie,Long Yongde,Mao Baoguang,Yu Zeqiang,Zhao Linjie,Tang Cheng,Kou Ruitian,Sun Chaoyang,Hu Chuangang
The oxide path mechanism offers vision possibility from kinetics for the anodic oxygen evolution reaction, yet its universal activation remains elusive due to the difficulty of constructing globally appropriate interatomic distances. Here, we address this challenge in the integrated-screened Y 0.1 Ir 0.2 Ru 0.7 O x by precisely regulating the Ru-Ru 1 bond length and constructing locally ordered yet long-range disordered structures that dissipate lattice strain, thereby enabling homogeneous modulation of interatomic distances across the framework. In-situ infrared spectroscopy together with online mass spectrometry confirms that the catalyst consistently follows a universal pathway. Building on these structural and mechanistic advantages, the nanostructured catalyst achieves a low cell voltage of 1.75 V@3 A cm -2 with operational stability ( > 2800 h @ 3 A cm -2 , 0.0625 mV h -1 decay rate) in proton exchange membrane water electrolysis. This work establishes a generalizable framework for pervasive dual-site catalysis, providing a paradigm for next-generation water electrolysis anodes and beyond.
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高影响因子
JOURNAL OF ELECTROCERAMICS
·
2023
·
IF 1.7
Li Wei,Li Jie,Chen Yixin,Sun Kai,Wang Shuai,Liu Yingli,Duan Zhaoyun
In this paper, the Ca 2+ ion was chosen to substitute the Mg 2+ ion of Li 2 MgSiO 4 ceramics. Li 2 Mg 1 − x Ca x SiO 4 (x = 0.0, 0.03, 0.06, 0.09, 0.12) ceramics materials were prepared by solid state reaction at low temperature (925 °C) with 2.5wt% Bi 2 O 3 sintering aid. X-ray diffraction (XRD) results showed the ceramics presented the standard Lithium Magnesium Silicate phase formation, and no secondary phases appeared. Scanning electron microscopy (SEM) suggested that Ca 2+ ions affected the densification of the prepared ceramics. Ca 2+ ion substitution resulted in increasing relative density, enhancing microwave properties. Ca 2+ ion substituted Mg 2+ ion and formed a CaO4 structure, which affected microwave dielectric properties. With the substituted amount increase, the values of dielectric constant ε’ and quality factor Q×f gradually increased, and τ f values increased from negative to positive values. When x = 0.09 and sintered at 925 °C, Ca 2+ ion substitution gave ceramics excellent microwave properties: bulk density ρ = 2.479 g/cm 3 , relative density was 98.68%, dielectric constant ε’=6.59, dielectric loss tanδ ε = 0.0018, quality factor Q×f = 8976.9 GHz and temperature coefficient τ f = 1.9 ppm/°C. This ceramic material has excellent microwave dielectric properties and holds a potential for use in integrated antenna and other electronic devices.
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危险品化学品经营许可证（带存储）