JOURNAL OF HAZARDOUS MATERIALS
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2025
·
IF 11.3
Ling Ma,Zi-Yang Fan,Wen-Qian Lian,Xin-Feng Wei,Rui-Ying Bao,Wei Yang
Embedding enzyme in biodegradable polyester accelerates hydrolysis in environments it ends up, but the release of microplastics (MPs) and nanoplastics (NPs) during this process remains underexplored. This work investigated the evolution of MPs and NPs released from poly(ε-caprolactone) (PCL) with embedded Lipase PS. The embedded enzyme significantly accelerated hydrolysis, causing the PCL film to disappear within 96 h. Notably, the formation rates and quantities of MPs and NPs were much higher compared to film with external enzyme. At 96 h, MPs (3.55 ×10 5 particles/mL) was 2.4 times, and NPs (4.65 ×10 7 particles/mL) was an order of magnitude higher than that with external enzyme. After 130 days, although both quantities and average size of MPs and NPs decreased due to only 90.6 % of enzymes were detected leaking, they did not completely disappear. The quantities of MPs and NPs were comparable to that with external enzyme, and the average size of MPs remained 1 μm. The simultaneous erosion inside film macroscopically, and severe chain cleavage microscopically, contributed to feasible film disintegration and formation of high amounts MPs and NPs. These findings underscore the importance of managing the release of MPs and NPs during the hydrolysis of enzyme-embedded biodegradable polyesters to ensure safety and mitigate environmental impact.
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LANGMUIR
·
2025
·
IF 3.9
Yi Sun,Wenlong Sun,KangKang Zhang,Siyu Cui,Xiong Shao,Jian Qiu,Yushan Yang,Jun Li
In order to improve the specimens’ flame-retardant qualities, ethyl orthosilicate was impregnated into them using a combination of the delignification process and the in situ growing approach. Lignification has been demonstrated to increase the cellular gap of the specimen and to maximize the impregnation of ethyl orthosilicate. Moreover, the in situ growth method results in the combination of specimens containing silica manufactured from ethyl orthosilicate, thereby forming a composite material with enhanced flame-retardant properties. The examination of the changes in the properties of the specimens before and after modification was conducted using FTIR spectroscopy, XRD, XPS, SEM, and other experimental techniques. The modified specimens exhibited higher properties in comparison to the untreated specimens. Furthermore, the modified specimens exhibited enhanced mechanical properties, characterized by an augmented compressive strength of 34.9 MPa and a strength-to-weight ratio of 158 MPa·cm·g–1, in comparison to specimens that did not undergo the modification process. The results of experiments show that proposed green technology provides a new method for producing flame-retardant composites and could significantly improve the mechanical and flame-retardant qualities of wood.
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IEEE SENSORS JOURNAL
·
2025
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IF 4.5
Chungang Wu,Xiaoming Chen,Ziang Bai,Qihang Ye,Bo Han,Maoqing Chen,Yingxuan Liu,Shuhua Ma,Yong Zhao
Microfluidics plays a significant role in various fields, including material synthesis, drug delivery, and disease diagnosis, and the flow velocity has a critical effect on the products. However, there is a lack of approaches for monitoring the flow velocity in the microchannel. To address this, we propose a novel fiber-optic flowmeter based on a flexible Fabry-Perot (F-P) cavity for online and non-invasive monitoring of products from the microfluidic devices. The flexible F-P cavity is constructed with a fiber-optic probe and gold-coated polydimethylsiloxane (PDMS) film, which can be easily integrated with versatile microfluidic devices through oxygen plasma bonding. Depending on the numerical investigation of film deformation under various film and fluid parameters, we experimentally characterize the sensitivity, reversibility, stability, and repeatability of this flowmeter. Then, the adaptability of the flowmeter is demonstrated by monitoring the flow velocity of seawater and sucrose solution. Moreover, we integrate this flowmeter with multiple microfluidic devices to verify the integration performance. Finally, we successfully achieve online and non-invasive monitoring of mixing efficiency, droplet diameter, and microalgae separation efficiency in the integrated microfluidic devices. This flowmeter can monitor the products from the microfluidic devices for disease diagnosis and drug delivery, with the advantages of good adaptability and easy integration.
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iScience
·
2021
·
IF 5.458
Zhikai Ye,Wenbo Li,Zhenlong Jiang,Erkang Wang,Jin Wang
Summary Although TGF-β signaling can effectively activate fibroblasts to transform to myofibroblasts, the underlying mechanisms involved in the cell fate switching for trans-differentiation have not been fully elucidated. In this study, we found the evidence of an intermediate state in the process of trans-differentiation. In the early stage of trans-differentiation, cells enter the intermediate state first with multiple characteristics such as accelerating cell cycle, metabolic switching, enhanced anti-apoptotic ability, and pluripotency, which is very similar to the early stage of reprogramming. As the trans-differentiation continues, these characteristics get switched. Therefore, trans-differentiation appears to require the switching of cell proliferation ability, metabolic pathway, and “stemness” to complete the process. In this study, we can conclude that an intermediate state may be necessary with high pluripotency in trans-differentiation from fibroblasts to myofibroblasts. Only after passing the intermediate state, the trans-differentiation is finally completed and will not easily return to the original state.
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LAB ON A CHIP
·
2017
·Vol. 17
·期 13
·2225-2234
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IF 6.045
Binfeng Hu,Juanjuan Li,Lei Mou,Yong Liu,Jinqi Deng,Wei Qian,Jiashu Sun,Ruitao Cha,Xingyu Jiang
Microfluidic platforms capable of automated, rapid, sensitive, and quantitative detection of biomarkers from patient samples could make a major impact on clinical or point-of-care (POC) diagnosis. In this work, we realize an automated diagnostic platform composed of two main components: (1) a disposable, self-contained, and integrated microfluidic chip and (2) a portable instrument that carries out completely automated operations. To demonstrate its potential for real-world application, we use injection molding for mass fabrication of the main components of disposable microfluidic chips. The assembled three-layered chip with on-chip mechanical valves for fluid control consists of (1) a top silicone fluidic layer with embedded zigzag microchannels, reagent reservoirs and a negative pressure port, (2) a middle tinfoil layer with patterned antibody/antigen stripes, and (3) a bottom silicone substrate layer with waste reservoirs. The versatility of the microfluidics-based system is demonstrated by implementation of a chemiluminescence immunoassay for quantitative detection of C-reactive protein (CRP) and testosterone in real clinical samples. This lab-on-a-chip platform with features of quantitation, portability and automation provides a promising strategy for POC diagnosis.
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Advanced Healthcare Materials
·
2016
·Vol. 5
·期 7
·786-794
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IF 5.76
Yanyan Huai,Shuai Dong,Ye Zhu,Xin Li,Binrui Cao,Xiang Gao,Mingying Yang,Li Wang,Chuanbin Mao
Candida albicans (CA) is a kind of fungus that can cause high morbidity and mortality in immunocompromised patients. However, preventing CA infection in these patients is still a daunting challenge. Herein, inspired from the fact that immunization with secreted aspartyl proteinases 2 (Sap2) can prevent the infection, it is proposed to use filamentous phage, a human-safe virus nanofiber specifically infecting bacteria (≈900 nm long and 7 nm wide), to display an epitope peptide of Sap2 (EPS, with a sequence of Val–Lys–Tyr–Thr–Ser) on its side wall and thus serve as a vaccine for preventing CA infection. The engineered virus nanofibers and recombinant Sap2 (rSap2) are then separately used to immunize mice. The humoral and cellular immune responses in the immunized mice are evaluated. Surprisingly, the virus nanofibers significantly induce mice to produce strong immune response as rSap2 and generate antibodies that can bind Sap2 and CA to inhibit the CA infection. Consequently, immunization with the virus nanofibers in mice dramatically increases the survival rate of CA-infected mice. All these results, along with the fact that the virus nanofibers can be mass-produced by infecting bacteria cost-effectively, suggest that virus nanofibers displaying EPS can be a vaccine candidate against fungal infection.
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ANALYST
·
2022
·Vol. 147
·期 12
·2757-2763
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IF 5.227
Xinying Zhang,Zhiwei Zhang,Chujing Yang,Ziheng Hu,Yingying Chen,Lingyan Feng
Signal amplification strategies increase the complexities of biosensors while improving the response signals. Herein, a novel electrochemical biosensor was developed based on a DNA hydrogel for sensitive analysis using microRNA-21 (miRNA-21) as a detection model. Poly C sequences combined with C–Ag(I)–C hydrogel formed a DNA hydrogel by the unique interaction between the cytosines and silver ions. Thus, with a three-way conjunction structure of DNA, this C–Ag(I)–C hydrogel was constructed as a novel biosensor for the detection of miRNAs. With the assistance of this hydrogel, numerous silver ions gathered around DNA strands, which would amplify the signal. Under these conditions, the silver ions produced distinct square wave voltammetry oxidation peak currents. This electrochemical biosensor we designed exhibited a great linear relationship for the logarithm of the concentration of miRNA-21 from 1 fM to 100 pM with a detection limit of 0.117 fM. Furthermore, our sensors were able to differentiate miRNA-21 from its homologous family with satisfactory responsiveness in the dilute bovine serum system.
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Collagen and Leather
·
2025
·Vol. 7
·期 1
·42
·
IF 9.2
Yi Xueling,Luo Duanyang,Liu Siqi,Chen Yanxu,Guo Jin,Yan Qingyu,Mao Hui
Antibiotic contamination has been a concerning environmental issue due to its potential threats to the development of drug-resistant bacteria and genes. Plant polyphenols were employed as biomolecule chelating ligands to graft onto collagen fibers, followed by the chelative immobilization of Fe ions. The as-obtained environmentally benign chelative biosorbent (CFBT-Fe) features a classic fibrous structure, excellent distribution and reservation of Fe ions, increased thermal stability, and inhibited swelling behavior owing to the introduction of bayberry tannin. An exceptional adsorption capacity of tetracycline on CFBT-Fe is exhibited across a broad pH range (3.0–10.0). Optimal adsorption capacity (31.30 mg/g) occurs at neutral pH 7.0, which is higher than many other similar biosorbents reported in the literature. The CFBT-Fe exhibits exceptional recyclability with sixth-cycle adsorption performance at 25.27 mg/g. The environmentally benign coordinative biosorbent is considered a promising candidate for the effective adsorptive removal of tetracycline from aquatic environments across a wide pH range. Graphical
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Nanoscale
·
2025
·Vol. 17
·期 39
·23010-23021
·
IF 5.1
Binqi He,Yunping Ma,Xinyue Xiang,Sifan Sun,Qianzuo Liu,Ting Zhang,Maiyong Zhu,Tie Li
The development of high-performance electrochemical catalysts capable of ultrasensitive detection of small molecules remains a significant challenge. Emerging two-dimensional materials, particularly metallenes such as Pd metallene, have demonstrated considerable potential in addressing these challenges. However, several critical limitations remain, including the need for further enhancement of electrochemical activity, detection sensitivity, and molecular selectivity. In this study, a one-step hydrothermal approach was employed to synthesize porous PdPr bimetallene alloys with a semi-transparent, wrinkled architecture, which function as high-performance catalysts in flexible electrochemical sensors. These materials exhibit excellent sensitivity and selectivity toward ethylene glycol detection. The incorporation of Pr not only induces unique synergistic lattice distortion effects but also enhances the thermodynamic stability of defect sites, which act as efficient electron-transfer channels and significantly activate the catalytic centers. This structural engineering significantly improves both electronic conductivity and structural stability, while also accelerating electron-transfer kinetics and enhancing the activity of the ethylene glycol oxidation reaction (EOR). This work not only demonstrates the effectiveness of active-site engineering in PdPr bimetallene for improved catalytic performance but also broadens the application of metallenes in the sensing of small molecules, aligning with current trends in advanced sensing technologies.
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LAB ON A CHIP
·
2025
·
IF 5.4
Zihan Wang,Fuwan Yang,Shuai Zeng,Rui Sun,Qifei Hu,Yichen Du
Applying CRISPR-based diagnostics to point-of-care pathogen detection remains challenging because of the multi-step and time-consuming sample preparation process. This study presents a low-cost, integrated valved microfluidic device that combines recombinase polymerase amplification (RPA), CRISPR signal amplification, and lateral flow readout for simultaneous nucleic acid detection. The core advantage of the platform lies in its ability to sequentially control the entire multi-step assay through simple valve operation, significantly minimizing user intervention. All key reagents, including the RPA mix, Cas12a/crRNA complex, and proteinase K lysis buffer, are pre-lyophilized, ensuring stability and ready-to-use functionality. The platform demonstrates a sensitivity of 20 copies/reaction for HPV16/18 plasmids and accurately genotypes HPV in lysates of cervical cancer cells within one hour, showing complete concordance with quantitative PCR results. This integrated device, achieving a user-friendly protocol and visual readout, provides a powerful tool for nucleic acid-based point-of-care testing and self-testing in resource-limited settings.
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Biosensors-Basel
·
2025
·Vol. 15
·期 12
·821
·
IF 5.6
Jie Chen,Shutao Liu,Sunyi Chen,Jingwen Mai,Maiwula Abudukadi,Yao Chen,Jie Lu,Guanglei Li,Chenchen Ge
Mycoplasma hominis (MH) is a prevalent opportunistic pathogen that is strongly associated with a wide range of urogenital tract infections and severe adverse pregnancy outcomes in clinical settings. Current MH detection methods, including microbial culture and qPCR, are time-consuming and rely on complex equipment, making them unsuitable for scenarios requiring rapid or simplified testing. In this study, we developed a visual readout biosensing platform by synergistically integrating recombinase polymerase amplification (RPA), CRISPR/Cas12a-mediated target nucleic acid recognition, and lateral flow biosensors for the rapid, sensitive, and specific identification of MH. The assay specifically targets the MH-specific 16S rRNA gene, achieving a limit of detection as low as 2 copies/reaction of recombinant plasmid containing the target gene with a total assay time of 60 min. Critical reaction parameters, including Cas12a-crRNA molar ratio, volume of RPA amplicon input, and Cas12a cleavage time, were systematically optimized to maximize the biosensor’s response efficiency and detection reliability. The platform exhibited exceptional specificity, with no cross-reactivity observed against common co-occurring urogenital pathogens, and effectively minimized aerosol contamination risks via a rigorous decontamination workflow. Furthermore, this work represents the first documented implementation of a contamination-control protocol for an MH-specific CRISPR-LFA assay. Notably, testing results from 18 clinical samples demonstrated the high specificity of this assay, highlighting its promising potential for clinical application.
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iScience
·
2025
·Vol. 29
·期 2
·
IF 4.1
Han-Ping Wang,Chen-Yu Xu,Wen Su,Yin-Song Wang,Yue Wang,Ming-Xin Cao
Periodontitis is a chronic inflammatory disease that leads to periodontal attachment loss and is a major cause of adult tooth loss. Current treatment of mechanical debridement is often ineffective in deep periodontal pockets, and antibiotic adjuncts carry the risk of bacterial resistance. Octyl gallate (OG), a natural plant-derived compound with antibacterial and antioxidant properties, is limited by poor water solubility and stability. This study aimed to develop a sustained-release ointment (OG-Ointment) using Pluronic F127 and ethylcellulose to enhance the delivery and efficacy of OG. The formulated OG-Ointment demonstrated excellent rheological properties, sustained drug release, and potent ABTS + radical scavenging. In vitro , it enhanced bacterial membrane permeability, inhibited P. gingivalis growth, and reduced ROS in macrophages. In a rat periodontitis model, OG-Ointment significantly reduced alveolar bone resorption, decreased ROS and inflammatory cytokines, and exhibited good biocompatibility. These findings suggest that OG-Ointment is a promising non-antibiotic adjunctive therapy for periodontitis, combining dual antioxidant and antibacterial functions with sustained release.
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