ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2026
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IF 4.1
Cao Ting,Li Mengjin,Ma Hong,Fan Zhefeng
The liver is a vital metabolic and detoxification organ in the body, yet the diagnosis of drug-induced liver injury (DILI) remains challenging due to the limitations of traditional detection methods. Monoamine oxidases (MAOs), which are highly expressed on the mitochondrial membrane of hepatic tissues, are closely associated with DILI. In this study, a near-infrared ratiometric fluorescent probe Mito-1 was developed based on the intramolecular charge transfer (ICT) and excited-state intramolecular proton transfer (ESIPT) mechanisms. This probe could precisely target mitochondria, exhibiting high sensitivity (DL = 0.14 μg/mL), a wide linear detection range (10–150 μg/mL), excellent selectivity towards MAOs, and good biocompatibility. Cellular experiments confirmed its ability to distinguish MAO activity between normal and liver cancer cells. In an acetaminophen (APAP)-induced DILI zebrafish model, Mito-1 enabled in vivo dynamic visualization of MAO activity in hepatic tissues. Collectively, this work provides a novel visual tool for the early diagnosis of DILI and the mechanistic study of MAOs. Graphical
The alternative text for this image may have been generated using AI.
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Nature Communications
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2026
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IF 18.1
Shu Yang,Chang Qing,Lin Ziying,Ko Pao-Fen,Chen Jingyi,Qiu Yuyueyang,Chen Feixiang,Zhao Zhen,Clark Glenn Thomas,Monreal Anette Vistoso,Ahn David,Liu Zhaoyang,Xu Jian,Chen Jian-Fu
Temporomandibular joint (TMJ) arthritis is a craniofacial disorder characterized by joint dysfunction and orofacial pain. Lymphatic regulation and function in TMJ remain unknown. Using genetic reporter mice, human tissues, tissue clearing, 3D volume imaging, and functional studies, we identified a synovial lymphatic system in TMJ. In a mouse model of TMJ arthritis, inflammation induces extensive lymphatic remodeling and leads to synovial lymphatic dysfunctions. Functional genetics and single-cell RNA sequencing (scRNA-seq) revealed that lymphatic deficiency induces a population of fibroblast-macrophage hybrid cells and enhances inflammation, exacerbating cartilage defects, bone loss, synovitis, and pain behaviors in TMJ arthritis mice. Conversely, lymphatic function promotion via a hydrogel-mediated VEGF-C delivery prevents TMJ pain, inflammation, and arthritis-like pathogenesis. Thus, we identified synovial lymphatics in TMJ and found that lymphatic dysfunction drives TMJ arthritis and pain, suggesting its potential as a therapeutic target.
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ACS ES&T Engineering
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2026
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IF 6.3
Xu Pan,Jiangkun Xie,Mingze Jiao,Liang Shan,Hongyuan Qi,Wenjun Huang,Zan Qu,Naiqiang Yan,Haomiao Xu
Methane selective catalytic reduction (CH4-SCR) represents a promising strategy for nitrogen oxides (NOx) abatement, yet its practical application is hindered by catalyst deactivation in the presence of water, caused by competitive adsorption on acid sites and irreversible hydroxylation of active metal species. In this study, we developed a dual-functional In@H-Beta/Fe2O3 catalyst through sequential ion-exchange and impregnation. This architecture confines indium species within the channels of H-Beta zeolite, while Fe2O3 nanoparticles are dispersed on the external surface, creating a synergistic system featuring a hydrophobic layer and strong Fe–O–In interfacial interactions. Under 5% H2O conditions, the optimized catalyst achieves 85% NOx conversion (vs 40% for conventional In@H-Beta). The enhanced water resistance is attributed to two factors: first, Fe2O3 establishes a physical barrier that protects acid sites and stabilizes InO+ active sites via Fe–O–In bonding, thereby inhibiting hydroxylation; second, strong interfacial Fe–O–In interactions further stabilize InO+ active sites against aqueous deactivation. Additionally, the Fe2+/Fe3+ redox cycle is proposed to facilitate O2 activation. In situ DRIFTS studies under 5% H2O confirm effective CH4 activation, as indicated by markedly more intense C–H stretching bands compared with In@H-Beta. The reaction pathway involves NO activation over Brønsted acid sites to form nitrate species, and CH4 activation on InO+ sites generating methyl radicals, which subsequently participate in NOx reduction. This work offers a new design strategy for developing highly active and water-tolerant CH4-SCR catalysts suitable for industrial flue gas treatment.
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Journal of the Taiwan Institute of Chemical Engineers
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2026
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IF 6.9
Wenjian Liu,Fei Wu,Yunyu Guo,Mengjiao Fan,Yan Tan,Yumeng Chen,Jiayue Zhu,Shu Zhang,Shuang Wang,Xun Hu
Background Aromatization is an important reaction route to form stable carbon skeleton for reserving pores generated during activation of biomass. Salts with Lewis acidity are known to catalyze dehydration and condensation reactions to form aromatic skeletons, while anions of salts are generally not inert and could influence evolution of pore development. Elucidation of the potential synergistic effects anions on pore evolution of activated carbon (AC) is of importance in optimizing the process for activation. Methods This study investigated the activation of peach wood with FeCl 3 , FeCl 2 , Fe 2 (SO 4 ) 3 or Fe(NO 3 ) 3 ) at 700°C, combined with in-situ IR spectroscopy to capture real-time dynamic change of reaction intermediates during activation process. Significant findings The results showed that Cl - in the iron salts uniquely stabilizes Fe 3+ (a distinctive advantage over other anions) for achieving efficient aromatization, yielding AC with the highest S BET (816.8 m 2 /g) and superior capability for removal of basic fuchsin (87.4%). FeCl 2 (Fe 2+ ) had weakened activity for aromatization, forming AC with loosely porous aggregates (597.6 m 2 /g). The activation with Fe 2 (SO 4 ) 3 reduced the sulfates and led to sulfur deposit blocking pores (S BET : 23.5 m 2 /g), with in-situ IR identifying S-O species that directly interfered with aromatization process. Fe(NO 3 ) 3 generated oxidative radicals that disrupted aromatization and wrapped Fe 3 O 4 species in inner structure, clogging pores (S BET : 230.8 m 2 /g). Increasing temperatures from 700 to 900°C in activation with FeCl 3 reduced the S BET progressively to 642.7 m 2 /g, resulting from excessive gasification, which also decreased AC yield to 17.4% and induced irreversible micropore-to-mesopore conversion, a temperature-dependent pore evolution pattern rarely highlighted in iron-salt activation systems. Notably, In-situ IR analysis revealed accelerated dehydration and aromatization at 300-400°C for consolidating microporous frameworks by Cl - and oxidation of carbonaceous intermediates by NO 3 - as well as formation of S-O species from SO 4 2- and their interference on aromatization. This work fills the knowledge gap in decoupling the roles of anions and iron valence in biomass activation, providing a targeted strategy for designing high-performance ACs with unique pore characteristics.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2026
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IF 3.9
Lemeng Wang,JiChao Hu,Jian Ma,Zedong Niu,YuChen Liu,Luanxiang Chao,Hemu Liu,Ruigeng Xie,Yuetong Zhao,Chenlu Mi,Kun Zhao,Dong Fu,Pengfei Qu,Pan Zhang
The industrial application of MEA-based carbon capture processes is significantly constrained by a low desorption rate and high energy consumption. To address these limitations, a NiCo@C catalyst was synthesized by using a hydrothermal-assisted impregnation method. The incorporation of Ni–Co dual doping into the carbon-based framework not only enhanced its stability and catalytic activity but also optimized its pore structure. A series of systematic characterizations were performed to evaluate the physicochemical properties of the material. The results indicate that the NiCo@C catalyst exhibits a substantially larger specific surface area and pore size compared to the Ni@C and Co@C catalysts. Additionally, the bimetallic catalysts introduce both weakly acidic and strongly acidic sites while maintaining a well-defined three-dimensional spherical structure. Compared to the noncatalytic MEA system, the introduction of NiCo@C increases the maximum desorption rate by 158% and the maximum desorption amount by 41.24%. These improvements contribute to a 53.48% reduction in energy consumption along with excellent cycle stability. The catalytic mechanism of NiCo@C was further investigated using 13C NMR, FT-IR, and Raman spectroscopy. The results reveal that bifunctional acid synergy in the solid NiCo@C catalyst promotes proton transfer and facilitates the breaking of C–N bonds, thereby enabling more efficient desorption with a lower energy consumption.
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FASEB JOURNAL
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2026
·Vol. 40
·期 4
·e71587
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IF 4.3
Yiya Wang,Yuan Shi,Yafei Zhang,Jing Shi,Zihan Wang,Jie Li,Jie Li,Zhenhui Liu,Wenzheng Wu,Liyan Wang,Hongzhao Sun
Hydrogen sulfide (H 2 S), a gaseous neurotransmitter, plays critical roles in neuromodulation and autonomic regulation within the central nervous system (CNS). The supraoptic nucleus (SON), one of a number of hypothalamic regions governing neuroendocrine secretion, also projects to autonomic centers to modulate systemic functions. Despite evidence of H 2 S activity in other brain regions, its specific role in the SON, particularly in regulating gastric motility, remains unexplored. Physiological saline (PS), L-cysteine (L-Cys, 2 nmol and 4 nmol), sodium hydrosulfide (NaHS, 2 nmol and 4 nmol), and combinations of NaHS with protein kinase A (PKA) inhibitor (H-89), p38 mitogen-activated protein kinase (p38-MAPK) inhibitor (SB203580), or adenylate cyclase-cyclic adenosine monophosphate (AC-cAMP) inhibitor (SQ22536) were microinjected into the SON of male adult Wistar rats ( n = 5/group). Changes in gastric motility were detected and compared. The microinjection of either L-Cys or NaHS into the SON significantly inhibited gastric motility, with a greater inhibitory effect observed at the higher dose of each substance ( p < 0.01). However, when H-89, SB203580, and SQ22536 were injected prior to the administration of NaHS, no significant changes in gastric motility were observed, thus indicating that these inhibitors completely counteracted the inhibitory effect of NaHS on gastric motility. The microinjection of endogenous or exogenous H 2 S into the SON of rats inhibits gastric motility. This inhibitory effect may be mediated through the PKA, AC-cAMP, and p38-MAPK pathways. Graphical Hydrogen sulfide (H 2 S) delivered via microinjection of its endogenous precursor L-cysteine (L-Cys) or exogenous donor sodium hydrosulfide (NaHS) into the rat supraoptic nucleus (SON) exerts inhibitory effects on gastric motility. This regulatory effect is mediated through the PKA, AC-cAMP, and p38-MAPK signaling pathways and can be reversed by the respective pathway inhibitors (H-89, SQ22536, SB203580).
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Advanced Science
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2026
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IF 14.1
Lili Lian,Zhenmin Le,Xuanqiao Ye,Zimo Wang,Jiuxiao Li,Chenyu Dong,Yetao Shen,Jiazheng Li,Yueping Ren,Xiaoyin Ma,Wei Chen,Qinxiang Zheng
Hyperosmotic stress induced by tear film instability significantly exacerbates oxidative damage in corneal epithelial cells, contributing to the pathogenesis of dry eye disease (DED). Ferroptosis, an iron-dependent form of cell death driven by lipid peroxidation, has been identified as a critical downstream mechanism of oxidative damage in DED. However, its precise regulatory mechanisms remain unclear. In this study, we demonstrate that hyperosmotic stress promotes ferroptosis in corneal epithelial cells by downregulating the NAD-dependent deacetylase sirtuin 1 (SIRT1). SIRT1 positively regulates GPX4, a pivotal mediator of ferroptosis. Pharmacological activation of SIRT1 using SRT1720 alleviated oxidative damage and suppressed ferroptosis in corneal epithelial cells both in vitro and in vivo. Mechanistically, we further observed that SIRT1 deacetylates the HIF1α, stabilizing it via the ubiquitin-proteasome pathway. The SIRT1–HIF1α axis positively regulates GPX4 levels, thereby inhibiting ferroptosis activation. These results reveal a previously unrecognized pathway of ferroptosis regulation in DED and suggest a potential therapeutic strategy for reducing oxidative damage in corneal epithelium.
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COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING
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2026
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IF 8.2
Yuanyuan Zhao,Shuimiao Xia,Qingyang Tang,Peng Yin,Hongqiang Wang,Runhua Fan,Zhicheng Shi
Ceramic dielectrics play a vital role in advanced electronic systems. However, the relatively low breakdown strength restricts the further application of ceramic dielectrics. To address this challenge, a novel vapor-phase in - situ deposition strategy is proposed in this study to enhance the breakdown strength of ceramic dielectrics. Specifically, carbon nanoparticles are uniformly introduced into the barium titanate (BT) ceramic matrix via the co-sintering of carbon black and pre-calcined ceramics. In this design, the Coulomb blockade effect of carbon nanoparticles suppresses charge transport to improve breakdown strength, while the micro-capacitor effect simultaneously enhances the dielectric constant. Experimental results show that the breakdown strength and dielectric constant (at 1 kHz) of the BT-4C ceramics reach 16.98 kV/mm and 4581, respectively, marking substantial increases of 49.9% and 49.5% relative to the pure BT counterpart. Further experiments demonstrate that when this strategy is applied to the linear strontium titanate (ST) ceramic system, the breakdown strength of ST-4C ceramics increases from 23.04 kV/mm (for pure ST) to 33.67 kV/mm, while the dielectric constant (at 1 kHz) simultaneously grows from 410.4 to 528.5. In this work, an efficient and practical strategy is proposed for the synergistic enhancement of the dielectric constant and breakdown strength of ceramic dielectrics.
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BIOSENSORS & BIOELECTRONICS
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2026
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IF 11.8
Man Xu,Meijin Li,Dianping Tang
Indole-3-acetic acid (IAA), one of the most important phytohormones, plays critical roles in plant growth, development, and stress response. However, minimally invasive and in-situ monitoring of IAA in living plants remains challenging due to the complex biological matrix and the lack of suitable in vivo sensing platforms. Herein, we report a minimally invasive microneedle electrochemical sensor for in vivo monitoring of IAA in plant leaves. A polymer microneedle array was fabricated and coated with a conductive Au layer, followed by electropolymerization of a molecularly imprinted poly(o-phenylenediamine) (poly-OPD) recognition film using IAA as the template molecule. The resulting microneedle sensor exhibited a selective electrochemical response toward IAA with good anti-interference capability against common electroactive plant metabolites. The sensor showed a linear response toward IAA over a wide concentration range with a low detection limit (0.44 μM). The microscale structure of the microneedles enabled minimally invasive insertion into plant tissues while maintaining structural integrity and stable electrochemical performance of the sensing platform. The developed sensor was further applied for in vivo monitoring of IAA fluctuations in tomato leaves under different physiological conditions. Distinct dynamic electrochemical response patterns were observed between normal and drought-stressed leaves, demonstrating the potential of the microneedle platform for plant physiological analysis and precision agriculture applications. This work provides a promising strategy for minimally invasive phytohormone sensing in living plants and expands the application of microneedle electrochemical devices in plant bioanalysis.
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JOURNAL OF FOOD SCIENCE
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2026
·Vol. 91
·期 6
·e71150
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IF 4.1
Hongan Li,Huanhuan Wang,Zeyuan Deng,Yao Pan,Hongyan Li
Flavored puffed bean products (FPBP) were primarily prepared from soybean flour or soybean flour supplemented with okara, which were mixed with sodium carbonate and water and subsequently subjected to a puffing treatment. In this study, the effects of the combined use of emulsifiers and moisture retention agents and incorporating okara into bean flour on the textural properties of puffed bean products were investigated. It was shown that both approaches effectively enhanced the springiness and resilience of FPBP while decreasing their hardness and cohesiveness. In vitro simulated digestion was performed on texture-enhanced FPBP. Determination of free amino acids revealed that FPBP exhibited a rich amino acid composition. Characteristic flavor compounds of the FPBP were identified using HS-SPME-GC–MS, and nine compounds with variable importance in projection (VIP) values exceeding 1 were selected as key flavor compounds. Furthermore, it was found that the texture-enhancing treatment effectively retained the unique flavor of FPBP and inhibited flavor degradation during storage. This research provided a theoretical basis and technical support for the optimization of production processes and flavor analysis of FPBP. Practical Applications This study helps improve flavored puffed bean snacks: adding specific additives or 20% okara keeps them springy and flavorful longer. They stay consistently “juicy” and tasty during shelf life, unlike regular snacks that harden or lose flavor. The method also works for other legume puffed snacks, aiding companies in developing new products efficiently.
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Cell Death & Disease
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2026
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IF 12.2
Ge Yutong,Yu Tao,Yu Shaokun,Sun Ao,Zhang Shiyu,Zhang Meiling,Wang Qian,Liu Jia,Zhang Xuan,Zeng Lulin,Lu Kaihua
Tumor-associated macrophages (TAMs) enriched in tumor microenvironment (TME) promote immune evasion and poor prognosis. Mitochondrial dysfunction, especially branched-chain amino acid (BCAA) metabolic reprogramming, has been confirmed to be involved in regulating TAMs function. The mitochondria-related protein MTFR2 was significantly upregulated in LUAD and closely associated with reduced survival. We conducted flow cytometry, multiplex immunofluorescence, immunohistochemistry on LUAD tissues, combined with analysis of public single-cell sequencing datasets, to characterize the TME features under MTFR2 dysregulation. Transcriptomics, metabolomics, and proteomics analyses revealed BCAA metabolic reprogramming, which was driven by MTFR2 binding to Leu125 within the β8 strand of VDAC1 and promoting its oligomerization. This interaction triggered mtDNA release into the cytoplasm, activating the TLR9-NF-κB pathway to upregulate BCAT1. Elevated concentrations of BCAA metabolites such as α-ketoisocaproate (KIC) and α-keto-β-methylvalerate (KMV) in the TME promoted M2 polarization and infiltration of TAMs by in vitro co-culture, 3D spheroid, and organoid models, and polarized M2 macrophages reciprocally promoted LUAD progression. Our findings establish a LUAD associated MTFR2-VDAC1-BCAT1 axis regulating the BCKAs-M2 axis in TAMs. Notably, targeting BCAT1 or depleting macrophages blocked this loop, offering a potential combination therapy for LUAD.
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JOURNAL OF PLANT PHYSIOLOGY
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2026
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IF 4.8
Mengyuan Cui,Xu Huang,Chenxu Zhao,Surya Kant,Shanshan Yang,Wenxuan Zhu,Xiaorong Fan,Shubin Sun,Guohua Xu,Xiaowen Wang
Minimizing the accumulation of polycyclic aromatic hydrocarbons (PAHs) in crops is crucial for human health. While OsNRT2.3b overexpression reduced phenanthrene (Phe) in rice, the underlying mechanisms and its response to complex conditions remained unclear. This study first confirmed a negative correlation between OsNRT2.3b expression and Phe utilizing 16 varieties. Further, to investigate whether the pH-sensing site H167 of OsNRT2.3b is the key, the wild-type (WT), OsNRT2.3b -overexpression (Ox), and H167-mutated OsNRT2.3b overexpression (H167R) were employed to expose to Phe under different nitrogen (N) and pH. Individual N and pH experiments demonstrated that H167 played a decisive role in suppressing Phe, with Phe in H167R being significantly higher than Ox. Moreover, both -N compared to + N, and pH 4.0 compared to 7.0, increased Phe while simultaneously enhancing H167-mediated regulation. Phe in Ox was significantly lower than H167R by 24.6% (-N) and 22.3% (pH 4.0). Combined pH and N treatments revealed that H167 was most effective under acidic and N-deficient conditions. Under hydroponic pH 4.0 & -N and pot soil pH 5.4 & LN, Phe in the roots of Ox were 19.7% and 17.3% lower than H167R. Effect size analysis indicated that an acidic environment exerted a stronger effect on H167-mediated Phe reduction than N under the conditions tested. Notably, in grains, the pH-specific mediation by H167 decreased Phe by 16.6% under acidic and N-deficient soil. This study provides a basis for mitigating Phe accumulation and associated PAH-related risks in rice through molecular strategies and soil pH and N management.
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危险品化学品经营许可证（带存储）