Surfaces and Interfaces
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2022
·
IF 6.137
Sihang Zhang,Jiangtao Xu,Zhichao Liu,Yingying Huang,Runfang Fu,Shouxiang Jiang
The synthesis of three-dimensional (3D) silver nanostructures with prominent plasmonic characteristics is of great importance in surface-enhanced Raman scattering (SERS) sensing applications. However, the preparation of 3D Ag nanostructures often suffer from high cost, complicated operation steps, time-consuming process and special instruments. In this work, 3D succulent-like silver nanoflowers (S-AgNFs) are successfully synthesized by a simple, scalable and low-cost liquid-phase reduction method at room temperature. The S-AgNFs with a diameter that ranges from 500 to 800 nm are composed of nano-petals that are about 60 nm in thickness. The growth process of S-AgNFs is systematically observed and investigated. And the solution-processed S-AgNFs are served as flexible SERS sensors via facile vacuum-assisted filtration onto bacterial nanocellulose (BNC) membranes. Attributed to unique 3D nanostructures of S-AgNFs, the as-prepared S-AgNFs@BNC SERS sensor with high-density and multi-level hotspots exhibits ultra-high detection sensitivity of 10 −13 M for malachite green (MG), impressive enhancement factor of 1.2 × 10 10 , good signal reproducibility and remarkable stability. Moreover, the flexible S-AgNFs@BNC SERS sensor also demonstrates excellent detection sensitivity of 10 −10 M for detecting pesticide residues on irregular surfaces. The flexible, stable and ultrasensitive S-AgNFs@BNC SERS sensor is promising for application in rapid identification of hazardous molecules, bacteria and viruses.
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高影响因子
Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 43
·8931-8944
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IF 7.571
Shengjie Ge,Yujie Wang,Zhiyue Li,Bin Lu,Jinhua Zhu,Hongmei Lu,Xiaowei Cao,Yayun Qian
In this study, we apply catalytic hairpin assembly (CHA) as the signal amplification strategy for the quantification of carcinoembryonic antigen (CEA) and cytokeratin fragment antigen 21-1 (CYFRA21-1) with a surface-enhanced Raman scattering (SERS) microfluidic chip (LoC-SERS) as the carrier. Herein, antibody–DNA conjugates are designed to assist the application of CHA amplification in protein detection. In the presence of protein biomarkers, antibody–DNA conjugates can specifically bind to the target proteins, forming the antigen@antibody–DNA conjugates. The terminal free part of the DNA on the conjugates can trigger the CHA events to connect SERS nanotags to capture nanoprobes. Then, micro-magnet can gather the CHA products in a rectangular chamber, resulting in the aggregation of SERS nanotags, which can ultimately generate abundant “hot spots” for SERS signal enhancement. Using this strategy, CEA and CYFRA21-1 can be successfully determined with a limit of detection (LOD) as low as pg mL−1, much lower than recently reported methods. Meanwhile, a non-small cell lung cancer (NSCLC)-xenografted mouse model was established, and SERS was applied to analyze the expression level of CEA and CYFRA21-1 in tumorigenesis and development. The comparison between SERS results and those of the ELISA method demonstrated a high degree of consistency, suggesting that the proposed CHA-assisted LoC-SERS device has satisfying accuracy. Thus, introducing the CHA strategy via the design of antibody–DNA conjugates opens new gates to ultra-sensitive and specific SERS detection of protein biomarkers.
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PubMed
高影响因子
Chemosensors
·
2022
·Vol. 10
·期 9
·373
·
IF 4.229
Junfang Hao,Min He,Bin Liu,Jianhui Yang
The preparation and self-assembling of monodisperse gold nanoparticles (Au NPs) is of great significance for its SERS application. According to the oleylamine-reduced method, oleylamine (OAm) serves as the reducing agent and stabilizing agent, and the effects of different reaction parameters such as solvent and temperature on the size and dispersity of Au NPs have been evaluated. The Au NPs synthesized with toluene as the solvent have the best dispersity and narrowest particle size distribution with adjustable sizes. The particle size gradually increases with the increase in reaction temperature. The highly ordered self-assembly film of Au NPs was employed as surface-enhanced Raman scattering (SERS) substrate for the probing molecule of rhodamine 6G. The Au substrate exhibits excellent spatial uniformity and SERS reproducibility, which indicates its practicability as a substrate. This study provides a simple synthesis strategy of highly ordered monodispersed Au NPs, which can serve as a SERS substrate with excellent spatial uniformity and SERS re-producibility.Keywords:gold nanoparticles;solvent effect;monodispersity;surface-enhanced Raman scattering (SERS)
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
·
IF 4.831
Yi He,Xiangjian Liao,Haonan Wu,Jialiang Huang,Yi Zhang,Yanyu Peng,Zhen Wang,Xin Cao,Caijun Wu,Xiaojun Luo
In this work, a simple and sensitive SERS-based miRNA biosensor was constructed based on porous MOFs nanoparticles and efficient subject-object recognition ability. MOFs as a container can package lots of signal probe neutral red (NR) for the advantages of three dimensional structure and porosity. The partially complementary duplex DNA can as a “lock” to lock up the hole for obtaining a weak Raman signal. In the present of miRNA, miRNA just like a “key” to open the duplex structure with the results of releasing NR. At this time, the released NR can be captured by SERS substrate [email protected] [7] for the subject-object recognition ability to produce a strong Raman signal which was positive correlation to target miRNA. By this way, the proposed SERS biosensor can achieve sensitively and selectively detect miRNA with a detection limit of 0.562 fM. This MOF-based SERS biosensor also be hopeful application for clinical diagnostics.
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高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Houjia Zhang,Nan Zhao,Hefu Li,Minghong Wang,Xuehui Hao,Meng Sun,Xiaojian Li,Zhenshan Yang,Huishan Yu,Cunwei Tian,Changzheng Wang
With the development of flexible surface-enhanced Raman spectroscopy (SERS) substrates that can realize rapid in situ detection, the SERS technique accompanied by miniaturized Raman spectrometers holds great promise for point-of-care testing (POCT). For an in situ detection strategy, constructing high-performance flexible and transparent SERS substrates through a facile and cost-effective fabrication method is critically important. Herein, we present a simple method for fabricating a large-area flexible and transparent SERS substrate consisting of a silver-nanoparticle-grafted wrinkled polydimethylsiloxane (Ag NPs@W-PDMS) film, using a surface-wrinkling technique and magnetron sputtering technology. By characterizing rhodamine 6G as a probe molecule with a portable Raman spectrometer, the flexible SERS substrate shows a low detection limit (10–7 M), a high enhancement factor (6.11 × 106), and excellent spot–spot and batch–batch reproducibilities (9.0% and 4.2%, respectively). Moreover, the Ag NPs@W-PDMS substrate maintains high SERS activity under bending and twisting mechanical deformations of over 100 cycles, as well as storage in air for 30 days. To evaluate its practical feasibility, in situ detection of malachite green on apple and tomato peels is performed with a detection limit of 10–6 M. In addition, for point-of-care analysis, we develop a wireless transmission system to transmit the collected SERS spectral data to a computer in real time for signal processing and analysis. Therefore, the proposed Ag NPs@W-PDMS SERS substrate fabricated through a simple and mass-producible method, combined with the utilization of a portable Raman spectrometer and wireless communication, offers a promising opportunity to extend the SERS technique from the laboratory to POCT applications.
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高影响因子
ANALYST
·
2022
·Vol. 147
·期 16
·3652-3661
·
IF 5.227
Qinghao Cao,Chao Chen,Jun Huang,Dantong Shen,Haolin Chen,Huiqing Zhong,Zhiming Liu,Zhouyi Guo
The flexible surface-enhanced Raman scattering (SERS) platform has ceaselessly propelled the development of point-of-care testing (POCT) in diverse fields. Herein, we report a facile strategy for the SERS-chemometric analysis of four β-blockers (bisoprolol, metoprolol, acebutolol and esmolol) based on a super-sticky mussel-inspired hydrogel SERS tape. The surface morphology and mechanical properties of the hydrogel tape can be easily controlled by adjusting the compositional ratio. The optimized tape with excellent toughness and adhesiveness allows efficient collection of analytes through a simple “paste and peel off” approach, further by spraying with silver nanoparticles using a household sprayer to instantly assemble a flexible SERS substrate, the analytes can then detected by a portable Raman spectrometer. This POCT strategy enables the identification and discrimination of four similar β-blockers with high sensitivity and accuracy in combination with the statistical algorithms. The developed SERS tape is finally utilized for the recognition of β-blockers in simulated urine solution, which realizes a limit of detection of 1.0 ng mL−1, revealing a promising potential of this SERS-based POCT for the clinical detection of doping abuse.
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高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 14
·2359
·
IF 5.719
Hao Fu,Weiwei Liu,Junqing Li,Wenguang Wu,Qian Zhao,Haoming Bao,Le Zhou,Shuyi Zhu,Jinglin Kong,Hongwen Zhang,Weiping Cai
Surface-enhanced Raman scattering (SERS) provides an unprecedented opportunity for fingerprinting identification and trace-level detection in chemistry, biomedicine, materials, and so on. Although great efforts have been devoted to fabricating sensitive plasmonic nanomaterials, it is still challenging to batch-produce a SERS substrate with high sensitivity, good reproducibility, and perfect recyclability. Here, we describe a facile fabrication of three-dimensional (3D) hierarchical Au/CuS nanocomposites, in which high-density Au nanotips enable highly SERS-active sensing, and the well-defined microflower (MF) geometry produces perfect signal reproducibility (RSD < 5%) for large laser spot excitations (>50 μm2), which is particularly suitable for practical on-site detection with a handheld Raman spectrometer. In addition, a self-cleaning ability of this Au/CuS Schottky junction photocatalyst under sunlight irradiation allows complete removal of the adsorbed analytes, realizing perfect regeneration of the SERS substrates over many cycles. The mass-production, ultra-sensitive, high-reproducibility, and fast-recyclability features of hierarchical Au/CuS MFs greatly facilitate cost-effective and field SERS detection of trace analytes in practice.Keywords:Au/CuS hybrids;high-density nanotips;SERS;signal reproducible;substrate recyclable
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Peihuan He,Md Mehedi Hassan,Wenjing Yang,Zhaoxia Shi,Xinyu Zhou,Yi Xu,Qin Ouyang,Quansheng Chen
Food safety is regarded as a crucial factor in both human health and economic progress. This study focuses on the fabrication of a label-free surface-enhanced Raman scattering (SERS) sensor for rapid sensing of three main mycotoxins (aflatoxin B1 (AFB1), ochratoxin A (OTA), ochratoxin B (OTB)) in rice using the optimized rough silver nanoparticles (AgNPs@K30) with enhancement factor (EF) 1.58 × 10 7 coupled multivariate calibration. Two variable selection chemometric calibration methods were comparatively applied. And genetic algorithm-partial least square achieved optimum correlation coefficient = 0.9797, 0.9779, and 0.9827, respectively for AFB1 ranging from 0.5 to 250 µg/Kg, for OTA and OTB 1 to 500 µg/Kg. The limit of detection (LOD) = 1.145, 1.133, and 1.180 µg/Kg, respectively, were determined according to principal component analysis-calibrated loading weight approach. And the great stability and reproducibility revealed the prepared SERS sensor has the potential to predict AFB1, OTA, and OTB in real rice samples.
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高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 6
·387
·
IF 5.743
Sen Li,Defu He,Shuning Li,Ruipeng Chen,Yuan Peng,Shuang Li,Dianpeng Han,Yu Wang,Kang Qin,Shuyue Ren,Ping Chen,Zhixian Gao
Bisphenol A (BPA) has emerged as a contaminant of concern because long-term exposure may affect the human endocrine system. Herein, a novel aptamer sensor based on magnetic separation and surface-enhanced Raman scattering (SERS) is proposed for the extremely sensitive and specific detection of trace BPA. Moreover, the capture unit was prepared by immobilizing thiolated (SH)-BPA aptamer complementary DNA on AuNP-coated magnetic halloysite nanotubes ([email protected]), and SH-BPA aptamer-modified[email protected]@Ag core–shell SERS nanotags acted as signal units. By the complementary pairing of the BPA aptamer and the corresponding DNA,[email protected]and[email protected]@AgCS were linked together through hybridization-ligation, which acted as the SERS substrate. In the absence of BPA, the constructed aptamer sensor generated electromagnetic enhancement and plasmon coupling to improve the sensitivity of SERS substrates. Owing to the high affinity between BPA and the aptamer, the aptamer probe bound to BPA was separated from the capture unit by an externally-induced magnetic field. Thus, the Raman intensity of the[email protected]@AuCS core–satellite assemblies was negatively correlated with the BPA concentration. High sensitivity measurements of BPA might be performed by determining the decline in SERS signal strength together with concentration variations. The proposed aptasensor is a promising biosensing platform for BPA detection.Keywords:surface-enhanced Raman scattering (SERS);aptasensor;bisphenol A;core–satellite assemblies
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高影响因子
NANOTECHNOLOGY
·
2022
·Vol. 33
·期 24
·245601
·
IF 3.953
Guangming Xu,Xilong Shen,Lei Zhang,Jie Tang,Chuan He,Ziqiang Zhou,Tian Xu,Meifeng Xu,Yonglong Jin,Chaonan Wang
In this study, we developed a flexible and transparent silver/polystyrene/polydimethylsiloxane (Ag/PS/PDMS) substrate with both high density of hot spots and satisfactory uniformity using a cost-effective approach. Via template-guided self-assembly, PS beads were arranged regularly in nanobowls of a square array on PDMS, whose surface structure was transferred from a commercial complementary metal oxide semiconductor chip. Roughness was introduced onto the PS bead surface by nitrogen plasma treatment, followed by sputtering of Ag which generated many hot spots. Differential roughness on the PS bead surface greatly influenced the morphology of the Ag/PS/PDMS substrate. A meat-ball like surface structure was formed with a plasma etching time of 5 min, whose growth mechanism was proposed based on the scanning electron microscope analysis. The high sensitivity and desirable uniformity of the meat-ball like Ag/PS/PDMS substrate were demonstrated by using crystal violet as a Raman reporter, exhibiting an enhancement factor of 2.7 × 107 and a relative standard deviation of 5.04%. Thiram of a lower concentration than the maximum residue limit on the cucumber surface could easily be detected in situ by the proposed substrate, demonstrating its great potential for in-situ food safety analysis.
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高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Xizhen Zhao,Yunqing Wang,Yunxia Ji,Rongchao Mei,Ying Chen,Zhiyang Zhang,Xiaoyan Wang,Lingxin Chen
Nanoplastics are regarded as inert particulate pollutants pose potential threat to organisms. It has been verified that they can penetrate biological barriers and accumulate in organisms; however, there is still a knowledge gap on the in vivo stability and degradation behaviors due to the lack of ideal analytical methods. Herein, a surface-enhanced Raman scattering (SERS) tag labeling technique was developed to study the in vivo behaviors of polystyrene (PS) nanoplastics by comparison with silica (SiO 2 ) nanoparticles (NPs). The labeled NPs were composed of gold NP core, attached Raman reporters as well as PS and silica shell, respectively, demonstrating strong SERS signals which were responsive to the compactness of the shells. The labeled NPs enabled the probing of in vivo structural stability of PS and silica in the liver, spleen and lung of mice after intravenous injection via the time-dependent evolution of SERS signal intensity and gold element content in the organs. The results indicated that both PS and silica model NPs retained in these organs without apparent excretion within 28 d. However, the structural stabilities of PS and silica differed dramatically as reflected by the SERS signal and tissue slice characterization. The silica shell completely degraded whereas the PS shell was still compact. Our results verified the long-term accumulation and in vivo inert property of nanoplastics, hinting that they were distinct from natural NPs and probably induce higher health risks from the aspect of the non-degradation property.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Laicui Song,Jianlin Li,Hao Li,Yunwei Chang,Shijie Dai,Ruimin Xu,Menghua Dou,Qianjin Li,Guangping lv,Tiesong Zheng
A highly sensitive surface-enhanced Raman spectroscopy (SERS) aptasensor for multiplex mycotoxins detection was developed based on aptamer-functionalized photonic microsphere array. Gold nanoparticles (AuNPs) were grafted covalently onto the silica photonic crystal microsphere (SPCM) to fabricate the SERS active substrate, on which the near-field enhancement can reach to 20 times analyzed by the simulation electric field distribution. Two dyes of 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) and nile blue A (NBA) were employed to make the SERS nanotags on AuNPs that were modified with corresponding anti-aptamer sequences. A SERS assay system was established based on the competitive reaction between the anti-aptamer on SERS nanotags and the target mycotoxin towards the aptamer on SPCM. The developed method showed a linear relationship in the range of 0.01–100 ng/mL for aflatoxin B 1 (AFB 1 ) and 0.001–10 ng/mL for ochratoxin A (OTA), and a low limit of detection of 0.36 pg/mL for AFB 1 and 0.034 pg/mL for OTA. The accuracy of such method was confirmed by its good recovery ratio and high repeatability, and further verified by the classic enzyme-linked immunosorbent assay using naturally mycotoxins contaminated edible samples. The established method has a great application potential for screening mycotoxins rapidly with high sensitivity, low-cost and high throughput in the fields of food and traditional Chinese herb.
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