CHEMICAL ENGINEERING JOURNAL
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2022
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IF 16.744
Hui Liu,Haoyuan Li,Siyuan Xia,Shanshan Yu,Yujie Duan,Lei Wang,Hongxiang Zhu,Hui He
The monitoring of intracellular pH value is beneficial for the early diagnosis and treatment of some diseases. Here, a cellulose nanocrystal (CNC)-based upconversion ratiometric fluorescent nanoprobe for pH monitoring and imaging was constructed by chemicaliy bonding the upconversion nanoparticles donor and fluorescein isothiocyanate and rhodamine B acceptors using CNC as a bridge. The CNC bridge could improve stability and solve the problems of aggregation-caused quenching of dye on the donor surface, long donor-to-acceptor distance, and poor water dispersibility of the probe. The probe with a double internal reference of donor and rhodamine B acceptor exhibited excellent selectivity, sensitivity and visualization for intracellular pH of 5.0–7.4 with 980 nm excitation. Interestingly, the chameleon-inspired biocompatible probe based on the luminescent resonance energy transfer platform enhanced the depth of penetration and demonstrated excellent pH monitoring and imaging in living cells and in vivo . Therefore, it has promising application for the early diagnosis and treatment of some diseases.
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OPTICS EXPRESS
·
2022
·Vol. 30
·期 24
·42801-42809
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IF 3.833
Guolu Yin,Guolu Yin,Hu Xiao,Hang Zhou,Tao Zhu,Tao Zhu
We propose a distributed pH sensor based on an optical frequency domain reflectometry using a PEGDA-based pH-sensitive hydrogel coated on a single mode fiber. The volume of hydrogel increased as pH value of the surrounding fluid decreased, which converts the pH value to the axial strain in the fiber. Taking capacity of distributed strain measurement with high spatial resolution in optical frequency domain reflectometry, the pH value of the external medium is distributed measured by the wavelength shifts of the local Rayleigh backscattering spectra. The basic hydrogel with different molecular weight was optimized to balance the sensitivity, the response time and also the stability. In the experiment, the range of the pH value from 2 to 6 was measured with a sampling resolution of 1.7 mm, a sensitivity of -199 pm/pH and a response time of 14 min when the hydrogel coating diameter is 2 mm. Such a distributed pH sensing system has a potential to detect and locate some chemical or biological substances in a large-scale environment.
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FOOD HYDROCOLLOIDS
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2023
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IF 10.7
Haifang Liu,Riting Huang,Xinyu Zhao,Shujuan Yang,Furui He,Wenqi Qin,Junhao Huang,Gaobo Yu,Yuhong Feng,Jiacheng Li,Chenghong Liao
The emulsion gels transformed by Pickering emulsions have great potential as a novel platform for oral drug delivery. In this study, an innovative alginate-based fluorescent colloidal particle was synthesized by Ugi reaction with alginate and carbon dots , which can stabilize Pickering emulsion at the present of Ca 2+ . The Pickering emulsions were converted to emulsion gels during gastric digestion. The microstructure of interfacial film was characterized in situ with confocal laser scanning microscopy. In the process of simulated gastrointestinal digestion, phase conversion of the Pickering emulsion was observed directly in situ by an automatic living cell imaging system. The results showed that clear fluorescent interfacial films can be visualized after simulated oral digestion, and fluorescent spheres were appeared after gastrointestinal digestion, indicating that Pickering emulsions were transformed into emulsion gels. Moreover, results on cell viability and cell uptake showed that the Pickering emulsion had good biocompatibility, which could be taken up by Caco-2 cells and could sustain the release of the drug. The formation of emulsion gels delays the digestion rate and improves the stability of encapsulated curcumin (CUR). In particular, in situ visualization technique also has promising applications in the digestion process and cellular uptake.
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JOURNAL OF MOLECULAR LIQUIDS
·
2023
·
IF 6
Yingxin Chen,Qian Chen,Richard M. Kasomo,Yanfeng Jin,Pujia Yang,Huifang Zheng,Xiaoqing Weng,Hongqiang Li,Shaoxian Song
Adsorption methods have garnered significant attention for fluoride removal from water bodies owing to their superior selectivity, simple operations, and wider applicability. However, the development of adsorbents with higher adsorption performances requires significant attention. Here, graphene oxide is endowed with excellent affinity towards fluoride by doping polyhydroxy complexes onto its surface structure and regulating the potential charge. Multiple detection measurements involving scanning electron microscopy (SEM) equipped with energy-dispersive X-ray spectroscopy (EDS), Brunauer-Emmett-Teller (BET) analysis, X-ray diffraction (XRD), and transmission electron microscopy (TEM) were employed to demonstrate the textural properties of graphene oxide (GO) modified by polyaluminium chloride (HAO@GO). Conversely, the effects of parameters including the preparation conditions, co-existing competitive ions, recyclability, contact time, and initial concentration on the adsorption behaviors were systematically determined. The adsorption experimental data were fitted well by the Langmuir isotherm model and pseudo-first-order model. The calculated maximum adsorption capacity was 129.23 mg/g at room temperature (25 °C) and a pH of 7.00, which is greater than that of most reported adsorbents. The existence of competitive anions, such as NO 3 - , SO 4 2 - , and PO 4 3 - , unavoidably interferes with fluoride adsorption. TEM, zero point of charge test, adsorption isotherm, and X-ray photoelectron spectroscopy (XPS) tests indicated that chemical adsorption and ligand exchange are responsible for the adsorption of fluoride onto HAO@GO.
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Materials Today Communications
·
2022
·
IF 3.662
Kai-fei Wang,Yi-ping Mu,Shu Wang,Yin-xue Song,Jing Xu,Xiao-hui Li,Rui-tao Wang
Pancreatic cancer is the most malignant tumor in the digestive system. Its strong invasiveness and difficulty of surgical removal result in a high mortality rate. We developed an erythrocyte membrane-camouflaged metal-organic framework ( [email protected] ) loaded with indocyanine green (ICG) for photothermal therapy of pancreatic cancer. ICG can be released in acidic tumor environments due to its pH responsiveness. More importantly, integrating erythrocyte membrane surface modification, immune escape, and prolonged blood circulation characteristics significantly improved the tumor-targeting ability of [email protected] The in vitro and in vivo results demonstrated that the prepared [email protected] exhibited a significant therapeutic effect on pancreatic cancer.
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ChemPlusChem
·
2022
·Vol. 88
·期 2
·e202200394
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IF 3.21
Yifei Zhou,Xuan Gao,Yu Lu,Ruohao Zhang,Kehong Lv,Jitong Gong,Jing Feng,Hongjie Zhang
Graphical A pH-responsive charge-convertible MTGZ@PPD drug delivery nanocarrier has been designed and fabricated for precise starvation and chemo synergistic oncotherapy. A pH-responsive charge-convertible drug delivery nanocarrier (MSN-TPZ-GOx@ZnO@PAH-PEG-DMMA, abbreviated as MTGZ@PPD) was prepared, which could specifically release hypoxia-activated chemotherapeutic Tirapazamine (TPZ) and glucose oxidase (GOx) in the tumor site for precise starvation and chemo synergistic oncotherapy. Acid-responsive Schiff base structure modified mesoporous silica nanoparticles (MSN) co-load with GOx and TPZ, then link with ZnO quantum dots (QDs). PAH-PEG-DMMA (PPD) polymer makes MTGZ@PPD with biocompatibility and charge-convertible feature. MTGZ@PPD is negatively charged at physiological pH, and the charge reversal of PPD and acidolysis of the Schiff base structure under the acidic tumor microenvironment (TME) induce a positively charged surface, which could potentiate the cell internalization. ZnO QDs could decompose at acidic TME, achieving controllable drug release. GOx could starve the tumor cells and enhance hypoxia level, thus initiates the activation of TPZ to realize synergistic starvation therapy and chemotherapy. This intelligent MTGZ@PPD has shown great potential for starvation and chemo synergistic oncotherapy.
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ACS Agricultural Science & Technology
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2022
·
IF 0
Yingjie Wang,Song Ma,Xin Yang,Yuhu Li,Shaoyu Lü
Controlled release formulations, used for the long-term preservation of pesticides, make agricultural development more precise, efficient, and safe. However, facile synthesis and high utilization of these formulations remain challenging. In this work, a polydopamine and metal–organic framework (MOF) hybrid was fabricated by a facile synthesis strategy for the controlled delivery of insecticide thiacloprid (THIA) and herbicide 2,4-D. The pesticides exhibited environment-responsive release behaviors. After 24 h, the cumulative release of THIA and 2,4-D reached 52.78 and 58.63% under alkaline conditions. The antiultraviolet photodegradation ability of THIA and 2,4-D increased nearly 4 times and 2 times, respectively. Significant insecticidal and herbicidal properties were observed for THIA@PDA-2,4-D@MIL-101(Fe). This hybrid achieves environmentally responsive and sustainable pesticide release by employing a dual cargo-carrying MOF and has great potential in intelligent, environmentally friendly, precise, and efficient pesticide delivery.
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Pharmaceutics
·
2022
·Vol. 14
·期 3
·535
·
IF 6.525
Huiru Zhu,Li Kong,Xu Zhu,Tingting Ran,Xiaojuan Ji
A high incidence of restenosis has been reported at the site of inflammation following angioplasty and stent implantation. The anti-proliferative drug paclitaxel (PTX) could help to reduce inflammation and restenosis; however, it has poor water solubility and serious adverse side effects at high doses. Given the presence of metabolic acidosis at the site of inflammation, we hypothesized that nanoparticles that are responsive to low pH could precisely release the loaded drug at the target site. We successfully constructed pH-responsive poly(D, L-lactic-co-glycolic acid) (PLGA) nanoparticles loaded with PTX and NaHCO3as a pH-sensitive therapeutic agent (PTX-NaHCO3-PLGA NPs). The NPs exhibited remarkable pH sensitivity and a good safety profile both in vitro in rat vascular smooth muscle cells and in vivo in Sprague Dawley rats after tail vein injection. In the rat model, the PTX-NaHCO3-PLGA NPs treatment group showed suppressed intimal proliferation following balloon-induced carotid artery injury compared with that of the saline-treated control. Overall, these results demonstrate that our newly developed pH-responsive nanodrug delivery platform has the potential to effectively inhibit restenosis.Keywords:vascular restenosis;pH-responsive nanoparticles;sodium bicarbonate;paclitaxel;drug delivery
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PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 2
·520
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IF 4.927
Le Xu,Xi Liu,Jiao Jia,Hao Wu,Juan Xie,Yongtang Jia
Accurately and sensitively sensing and monitoring the pH in the environment is a key fundamental issue for human health. Nanomaterial and nanotechnology combined with fluorescent materials can be emerged as excellent possible methods to develop high-performance sensing membranes and help monitor pH. Herein, a series of fluorescent nanofiber membranes (NFMs) containing poly-1,8-naphthimide derivative-3-[dimethyl-[2-(2-methylprop-2-enoyloxy)ethyl]azaniumyl]propane-1-sulfonate (PNI-SBMA) are fabricated by electrospinning the solution of PNI-SBMA blended with poly(vinyl alcohol) (PVA). The surfactant-like functionalities in side chains of PNI-SBMA endow the NFMs with outstanding hydrophilicity, and the naphthimide derivatives are sensitive to pH by photoinduced electron transfer effect, which contribute to highly efficient pH fluorescence sensing applications of NFMs. Specifically, the PNI-SBMA/PVA NFM with a ratio of 1:9 (NFM2) shows high sensitivity and good cyclability to pH. This work demonstrates an effective strategy to realize a fluorescent sensor NFM that has a fast and sensitive response to pH, which will benefit its application of pH sensor monitoring in the water treatment process.Keywords:electrospinning;nanofibers;fluorescence;sensors;pH
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MARINE POLLUTION BULLETIN
·
2022
·
IF 7.001
Wang Li,Bo Zu,Qingwei Yang,Junwen An,Jiawen Li
Nanoplastics (NPs) are widely found in the environment and can act as a vector for various toxic substances and promote their diffusion and bioenrichment, but the underlying mechanisms are largely unknown. Here, the adsorption characteristics of bisphenol A (BPA) onto NPs were explored. The results show that the adsorption of BPA on NPs was dominated by saturated single-layer adsorption and affected by both intra-particle diffusion and liquid film diffusion. Electrostatic interaction, π–π interaction, and hydrophobic effects played key roles in adsorption. In addition, the introduction of electrolytes inhibited the adsorption of BPA onto NPs. Interestingly, the introduction of suspended sediment promoted the formation of heterogeneous aggregates of NPs-SS, thereby reducing the adsorption capacity, indicating that aggregation may play an important role in the adsorption behavior of NPs. Overall, our results provide new insights into the adsorption behavior of BPA on NPs and the underlying mechanisms under different environmental conditions.
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高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2022
·Vol. 38
·期 5
·1-8
·
IF 4.253
Li Yudi,Chen Guiying,Ge Fanglan,Dang Ting,Ren Yao,Zeng Bei,Li Wei
γ-aminobutyric acid (GABA) has various physiological functions and is widely used in medicine, food, and other fields. Glutamate decarboxylase (GAD) is a key enzyme that catalyzes the decarboxylation of L-glutamate to synthesize GABA. However, the industrial application of microorganism-derived GAD is limited by its rapid loss of enzymatic activity with pH approaching neutrality. In this study, a novel glutamate decarboxylase, GAD MSM, from Mycobacterium smegmatis was overexpressed and purified. On the basis of homologous modeling and substrate molecular docking, several GAD MSM mutants were constructed, and their enzymatic properties were analyzed. The results showed that the optimal pH of wild-type GAD MSM is 5.4; at pH 6.2, 22.8% enzymatic activity was retained. The T211I replacement in GAD and C-terminal deletion mutant GAD MSMΔC showed relatively high catalytic activity in a pH range of 5.0–7.0. The V max and K m values of GAD MSMΔC were 14.69 and 5.70, respectively, at pH 5.5, and 9.87 and 6.17, respectively, at pH 7.0. Compared with the wild-type GAD, GAD MSMΔC maintained higher affinity and enzymatic activity of the substrate, maintaining 78.5% of the highest enzymatic activity even at pH 7.0, which is the highest reported activity retention for GAD under neutral pH condition. Therefore, GAD MSMΔC can be used for the transformation of high-yielding strains and industrial production of GABA.
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DYES AND PIGMENTS
·
2022
·
IF 5.122
Shanshan Li,Jiaqi Zhang,Yanrong Li,Yang Xu
Synthesis of nano coordination polymers (NCPs) with dual fluorescence emission is highly desirable, but is still a challenge for further ratiometric sensing applications. In this work, porphyrin-based NCPs with dual fluorescence emission were prepared via a solvothermal process using branched polyethyleneimine (PEI) as passivation agent for ratiometric fluorescent sensing of pH and copper ion . Two NCPs@PEI can be obtained with different fluorescence emissions due to regulation of reaction time. NCPs@PEI-1 exhibited a good fluorescence response to pH with a calibration function of l g ( I 655 / I 477 ) = 0.2750 ( p H ) − 2.2989 (R 2 = 0.977), derived from protonation and deprotonation of pyrrolic nitrogen and amino groups in the NCPs@PEI. Moreover, by exploiting the electron transfer between Cu 2+ and nitrogen in porphyrin , NCPs@PEI-1 was used as sensor for detecting Cu 2+ with a function of y = − 0.0074 x + 0.5990 (R 2 = 0.999) and the limit of detection was calculated to be 136 nM based on 3σ/κ. These NCPs with tunable emission, excellent fluorescence, and “two in one” characteristic for Cu 2+ and pH sensing highlights NCPs@PEI as a potential fluorescent probe for multi-target analysis.
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