Foods
·
2024
·Vol. 13
·期 9
·1338
·
IF 4.7
Ruimin Teng,Cun Ao,Haitao Huang,Daliang Shi,Yuxiao Mao,Xuxia Zheng,Yun Zhao
Longjing tea is favored by consumers due to its refreshing and delicate aroma, as well as its fresh and sweet flavor. In order to study the processing technology of Longjing tea with ‘Baiye 1’ tea varieties, solid phase microextraction and gas chromatography–mass spectrometry were used to analyze the volatile components of Longjing tea in different process stages. The results revealed the identification of 275 aroma metabolites in the processing samples of Longjing tea. The sensory evaluation and principal component analysis revealed that the leaves of fresh (XY) and spreading (TF) were different from the leaves of first panning (YQ), second panning (EQ), final panning (HG), and fragrance enhancing (TX). The relative contents of geraniol (1199.95 and 1134.51), linalool (745.93 and 793.98), methyl salicylate (485.22 and 314.67), phenylethyl alcohol (280.14 and 393.98), 2-methylfuran (872.28 and 517.96), 2-butenal (56.01 and 154.60), and 2-hexenal (46.22 and 42.24), refreshing and floral substances in the XY and TF stages, were higher than other stages. The aroma contents of 2-methylfuran, furfural, 2-methyl-1-penten-3-one, 3-hexen-2-one, dodecane, hexanoyl hexanoate, 2,5-dimethyl-pyrazine, and methyl-pyrazine were found to be significantly positively correlated with the intensity of chestnut aroma. In conclusion, this study contributes to a better understanding of the composition and formation mechanism of chestnut-like aroma and provides new insights into the processing technology to improve the quality of albino green tea.
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AMB Express
·
2024
·Vol. 14
·期 1
·1-16
·
IF 3.5
Yan Yan,Yuan Haisheng,Yang Fan,Na Heiya,Yu Xiuling,Liu Jingran,Wang Yuzhen
Non-alcoholic fatty liver disease (NAFLD) is becoming a significant global public health threat. Seabuckthorn ( Hippophae rhamnoides L.) has been used in traditional Chinese medicine (TCM). The hypolipidemic effects of Seabuckthorn polysaccharides (SP) against high-fat diets (HFD)-induced NAFLD were systematically explored and compared with that of Bifidobacterium lactis V9 ( B. Lactis V9). Results showed that HFD-induced alanine transaminase (ALT) and aspartate aminotransferase (AST) levels decreased by 2.8-fold and 4.5-fold, respectively, after SP supplementation. Moreover, the alleviating effect on hepatic lipid accumulation is better than that of B. Lactis V9. The ACC and FASN mRNA levels were significantly reduced by 1.8 fold ( P < 0.05) and 2.3 folds ( P < 0.05), respectively, while the CPT1α and PPARα mRNA levels was significantly increased by 2.3 fold ( P < 0.05) and 1.6 fold ( P < 0.05), respectively, after SP administration. SP activated phosphorylated-AMPK and inhibited PPARγ protein expression, improved serum oxidative stress and inflammation ( P < 0.05). SP supplementation leads to increased hepatic expression of nuclear factor erythroid 2-related factor 2 (Nrf-2), heme oxygenase-1 (HO-1) and Superoxide dismutase-2 (SOD-2). Furthermore, SP treatment improved HFD-induced intestinal dysbiosis. Lentisphaerae , Firmicutes , Tenericutes and Peptococcus sp., RC9_gut_group sp., and Parabacteroides sp. of the gut microbiota were significantly associated with hepatic steatosis and indicators related to oxidative stress and inflammation. Therefore, SP can mitigate hepatic lipid accumulation by regulating Nrf-2/HO-1 signaling pathways and gut microbiota. This study offers new evidence supporting the use of SP as a prebiotic treatment for NAFLD.
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Toxics
·
2025
·Vol. 13
·期 8
·662
·
IF 4.1
Jiahong Jiang,Qi Liu,Boxiang Zhang,Lei Zhao,Dan Xu
Sodium p-perfluorous nonenoxybenzenesulfonate (OBS) has been proposed as a substitute for perfluorooctanesulfonic acid (PFOS), yet it has garnered increasing attention due to its environmental persistence and potential toxicity. Despite these concerns, the neurotoxic mechanisms of OBS remain unclear. This study investigates and compares the neurotoxic effects and mechanisms of OBS and PFOS inCaenorhabditis elegans. L4-stage worms were exposed to OBS (0.1–100 μM) or PFOS (100 μM) for 24 h. Neurobehavioral analysis showed that OBS exposure induced concentration-dependent neurobehavioral deficits, with 100 μM OBS significantly reducing pharyngeal pumping rate (29.8%), head swing frequency (23.4%), and body bending frequency (46.6%), surpassing the effects of PFOS. Both compounds decreased the fluorescence intensity of dopaminergic, glutamatergic, and γ-aminobutyric acid neurons and downregulated neurotransmitter-associated genes. They also increased ROS generation and inhibited antioxidant gene expression. Molecular docking revealed that OBS had a stronger binding affinity to p38 MAPK key protein (PMK-1) than PFOS. OBS and PFOS upregulatedpmk-1andskn-1, modulating oxidative stress and neuronal function.pmk-1mutation differentially affected OBS-induced neurobehavioral changes and gene expression alterations. Our findings indicate that OBS exhibits stronger neurotoxicity than PFOS inCaenorhabditis elegans, mediated through the PMK-1 pathway. These results highlight the need for further investigation into the safety of OBS as a PFOS alternative.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2025
·
IF 3.6
Xiong Neng,Chen Jia-Yu,Lei Pei-Qi,Fu Teng-Teng,Xu Rui,Xue Ya-Ping,Zheng Yu-Guo
Immobilized enzyme bioreactors provide a controlled reaction environment and integrated reaction-separation processes for biocatalysis. In this study, a biocatalytic process based on resin-immobilized nitrilase and a partial-mixed recirculating bed bioreactor was developed for the synthesis of 1-cyanocyclohexaneacetic acid, a gabapentin intermediate. The resin catalyst prepared by immobilizing the regioselective nitrilase AcN-S on the activated amino resin LX-1000EPHA achieved 99.12% immobilization yield, 73.40 U/g specific activity, and 95.42% activity recovery when loaded with 10 mg/g crude enzyme. The resin catalyst (100 g/L) could convert 1 M (148.2 g/L) substrate 1-cyanocyclohexaneacetonitrile to 1-cyanocyclohexaneacetic acid within 18 h, achieving a conversion of 95.40%. At a substrate concentration of 0.5 M, > 85% conversion could still be achieved after 15 batches. In a partial-mixed recirculating bed reactor, the resin catalyst (100 g/L) could completely convert 500 mM substrate within 10 h, and achieve > 90% conversion after 20 batches, with residual activity of 93.23%. Resin activation and cross-linking treatment after immobilization were found to improve operational stability, reduce protein leakage, and ensure high immobilization yield and activity recovery. The reactor provided a low-shear environment and recirculating flow, which together improve catalyst reusability and reduce product inhibition. The constructed reaction system provides a solution for the efficient conversion of slightly soluble/insoluble substrates and the integration of reaction and separation.
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JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
·
2025
·
IF 5.4
Chunying Jiang,Shudan Yang,Yuting Wang,Liyuan Du,Miao-Miao Niu,Dongli Zhang
Poly (ADP-ribose) polymerase 1 (PARP-1) exhibits high expression levels in colorectal cancer (CRC) patients and participates in multiple DNA damage repair pathways, thereby emerging as an attractive target. Herein, we identified a series of PARP-1 inhibitors (termed as compounds 1-6) by pharmacophore modelling, virtual screening and biological evaluation. Enzyme inhibition assays demonstrated that compound-5 significantly inhibited PARP-1 activity (IC50 = 0.07 ± 0.01 nM) and exhibited high selectivity for PARP-1 among 63 different kinases. Molecular dynamic simulations indicated that compound-5 stably bound to the catalytic domain of PARP-1. Cellular assays demonstrated that compound-5 significantly inhibited the proliferation of a panel of human CRC cell lines (HCT116, SNU-1, Caco-2, HT-29). The data suggest that compound-5 may be a highly potent and selective PARP-1 inhibitor for CRC therapy.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2025
·
IF 8.3
Mingxin Cai,Qinghua Ye,Ziru Wang,Peilei He
Electrochemical water splitting for hydrogen production is currently constrained by the sluggish kinetics of the anodic oxygen evolution reaction (OER). NiFe-based catalysts have emerged as promising alternatives to precious metal OER catalysts due to their low cost and relatively high electrocatalytic activity. However, NiFe-based catalysts prepared by conventional synthesis methods suffer from a low density of exposed active sites and ambiguous structures. Herein, we report a straightforward solution-thermal strategy for constructing two-dimensional sub-1 nm nanosheets (SNSs) through the self-assembly of an iron-substituted polyoxometalate (FeMo 6 ) cluster with Ni 3 S 2 . The Ni 3 S 2 –FeMo 6 SNSs exhibit excellent electrocatalytic performance in 1 M KOH, achieving an overpotential of only 342 mV at a current density of 100 mA cm −2 and maintaining stability for 100 h at 10 mA cm −2 . The synergistic effect between Fe and Ni 3 S 2 effectively modulates the electronic structure of the catalyst, accelerating the formation of the active NiOOH phase. Furthermore, Fe incorporation actively enhances hydroxyl groups adsorption on the catalyst surface and facilitates lattice oxygen participation in the reaction. This work clarifies the role of Fe in NiFe-based electrocatalysts, providing new perspectives for developing facile-to-synthesize and high-performance electrocatalysts.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Jia Yao,Pengtao Chen,Dingqi Xie,Jianqingchen Chen,Xiao Lin,Yujin Xu
Radiotherapy (RT) is a widely used clinical treatment for cancer, leveraging ionizing radiation; however, some patients exhibit resistance to radiation due to insufficient reactive oxygen species (ROS) production. Copper-based nanomaterials represent a promising class of antitumor agents capable of generating abundant ROS. In this study, R837 and Cu2–xSe were incorporated into PLGA (50:50)20k-PEG2k-iRGD to develop a targeted nanomaterial for tumor therapy. These nanoparticles effectively induced cuproptosis, inhibited tumor cell proliferation, remodeled the tumor immune microenvironment (TME), and promoted immunogenic cell death (ICD), thereby enhancing the efficacy of PD-1 checkpoint blockade therapy (αPD-1). Furthermore, our results demonstrated that the Cu2–xSe(R837)-iRGD nanoformulations, in combination with radiotherapy, promoted the infiltration of CD8+ T cells and activated antitumor immune responses in vivo. In conclusion, our study confirmed that Cu2–xSe(R837)-iRGD nanoformulations combined with radiotherapy yielded promising therapeutic outcomes and held potential for clinical application in osteosarcoma (OS) treatment.
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ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Sijie Yuan,Ying Tu,Ru Yu,Fei Nie
Introducing novel catalysts is essential for developing chemiluminescence (CL) systems that exhibit sustained and robust emission. Traditional Luminol-H2O2 systems typically feature flash-type CL emission. In this study, we discovered that the porous material HKUST-1 can induce a long-lasting and intense CL emission when combined with Luminol-H2O2. This long-term emission signal can be directly detected by the smartphone. By changing the calcination temperature, a series of microporous and hierarchically porous HKUST-1 materials were prepared as catalysts to adjust the kinetic characteristics of the CL signal of Luminol-H2O2 system from flash-type to glow-type. A systematic investigation into the influence of the central metal and ligand, aperture, and particle size of HKUST-1 on the CL kinetic properties revealed that the pore structure has the most pronounced impact on the dynamics of the Luminol-H2O2 CL reaction. Capitalizing on the intense emission of the HKUST-1-catalyzed Luminol-H2O2 system, we established a CL sandwich immunoassay strategy for concanavalin A (ConA), demonstrating good linearity and low detection limit. This research presents a significant endeavor in modulating the dynamics of CL signal emissions.
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Translational Neurodegeneration
·
2025
·Vol. 14
·期 1
·1-27
·
IF 15.2
Wang Jie,Ni Hong,Wang Yu,Wei Luyao,Ding Hanqing,Guo Zhongzhao,Pan Hao,Yu Ying,Luo Jia,Pan Weidong,Wang Deheng,Ke Zun-Ji
Background Alzheimer's disease (AD) is a neurodegenerative disease with major symptoms including memory and learning deficits. Neuroinflammation associated with reactive microglia promotes AD progression. These reactive microglia secrete prostaglandins, which are synthesized through the enzymatic activity of cyclooxygenase (COX)-1 and COX-2. Here, we aimed to elucidate the specific mechanisms of COX1 in AD pathogenesis and its interactions with neuroinflammatory processes.Methods We conducted backcrossing between COX-1 knockout (KO) and 5 × FAD mice to evaluate the effect of COX-1 deficiency on neuroinflammation. In addition, single-cell sequencing and microarray datasets from public databases and ingenuity pathway analysis in vitro were employed to explore gene expression profiles in the brains of AD mice.Results We identified a significant upregulation of COX-1 in 5 × FAD mice, with expression specifically localized to microglia in an age-dependent manner. Additionally, COX-1 KO alleviated neuroinflammation and accumulation of Aβ plaques, subsequently improving cognitive behavior in 5 × FAD mice. Moreover, microglia exhibited an amoeboid morphology in 5 × FAD mice, whereas in age-matched 5 × FAD/COX-1 KO mice, microglia had a ramified appearance. Additionally, our study demonstrated a pharmacological approach that inhibits the prostaglandin E2 (PGE2)/EP2 receptors via inhibition of the cAMP-PKA-NFκB-p65 pathway and NLRP3 inflammasome activation, producing similar beneficial effects as observed in COX-1 KO mice.Conclusion Our findings indicate that targeting the COX-1/PGE2/EP2 signaling pathway may alleviate neuroinflammation and impede AD progression. Moreover, the EP2 receptor presents a promising pharmacological target for mitigating the pathological effects associated with COX-1 activity in AD patients.
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Translational Cancer Research
·
2025
·Vol. 14
·期 10
·6454
·
IF 1.7
Jiaxuan Zhao,Han Wu,Zhe Sun,Yeting Cui,Nan Liu,Yixuan Wang,Youyong Wang,Jiangzhou Shi,Tongcun Zhang
Background Chimeric antigen receptor T cell (CAR-T) therapy targeting cluster of differentiation 19 (CD19) has shown promise in treating B-cell acute lymphoblastic leukemia (B-ALL), but the long-term efficacy of CD19-targeted CAR-T therapy remains limited. Programmed cell death protein 1 (PD-1), an inhibitory receptor on activated T cells, engages programmed death ligand 1 (PD-L1) in the tumor microenvironment, suppressing CAR-T cell function by impairing key signaling pathways. To address this limitation, we investigated the effect of short hairpin RNA (shRNA)-mediated PD-1 knockdown on alleviating CAR-T cell exhaustion, preserving memory phenotype, and enhancing the long-term efficacy of CD19-targeted CAR-T cells. Methods To overcome this limitation, we employed shRNA to achieve PD-1 knockdown in CD19-targeting CAR-T cells. Following screening multiple shRNA candidates, we assessed the cytotoxicity, cytokine secretion, and proliferation of PD-1 knockdown CAR-T cells. Their memory phenotype, exhaustion marker, and cytolytic function were further evaluated after repeated antigen stimulation. Therapeutic efficacy and safety were validated in NOD.Cg-PrkdC scid Il2rg tm1 /Vst (NPG) mice. Results PD-1 knockdown significantly enhanced CAR-T cell cytotoxicity and persistence against PD-L1+ RAJI (human Burkitt’s lymphoma cell line) cells. After three rounds of antigen stimulation, PD-1 knockdown CAR-T cells sustained cytotoxic activity, retained a memory-like phenotype, and displayed attenuated exhaustion markers. In vivo , they prolonged survival without inducing cytokine release syndrome (CRS), indicating improved efficacy and safety. Conclusions PD-1 knockdown mitigates tumor-induced T-cell exhaustion and represents a promising strategy to enhance the durability and potency of CAR-T cell therapy in cancer treatment.
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Nature Communications
·
2025
·
IF 15.7
Peng Xudong,Zhang Yang,Wang Qiyu,Chen Lu,Chen Nanshan,Jiang Yanxin,Yang Zhiqiang,Pan Hongji,Li Hongxu,Zhao Tianshou,Li Yiju
Emerging applications, such as electric aircraft and ships, demand rechargeable batteries with high specific energy. However, current lithium (Li) ion batteries fall significantly short in this regard. Even with Li metal batteries, achieving a high cell-level specific energy (e.g. ≥600 Wh kg −1 ) remains a significant challenge. In this study, a synergistic strategy integrating a precisely engineered carbonate-based gel-solid-state electrolyte with a surface-modified Li is proposed. Our in-situ gelation technique confines unstable free carbonate solvent molecules, thereby suppressing the electrolyte-induced parasitic reactions. Furthermore, a bicontinuous gradient polymer layer that regulates Li deposition and improves moisture/oxygen resistance is introduced, enabling damage-free Li processing and avoiding harsh production requirements. As a result, our 11 Ah-level Li metal battery achieves a high specific energy of 604.2 Wh kg −1 (626.4 Wh kg −1 excluding the packaging materials) and operates for over 100 cycles with an energy retention of 92.83% under lean electrolyte conditions (0.85 g Ah −1 ). This work offers a practical strategy for the scalable production of long-cycle Li metal batteries with high specific energy for broad future applications.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2025
·Vol. 36
·期 31
·1-11
·
IF 2.8
Li Shanshan,Zhang Duo,Tian Zhongqing,Wang Fei,Meng Fancheng
Ba[(Zn 0.6 Mg 0.4 ) 1/3 Nb (2- x )/3 Sb x /3 ]O 3 (BZMNS, x = 0.15, 0.2, 0.25, 0.3, 0.35) ceramics were synthesized by the conventional solid-state reaction method. The crystal structure and microwave dielectric properties of the ceramics were investigated. X-ray diffraction (XRD) analysis revealed that the BZMNS ceramics exhibit a cubic perovskite structure. The crystal structures were refined, and no secondary phases were detected in all compositions. The doping of Sb 5+ hinders grain growth, and the average grain size first increases and then decreases with the increase of the x value. The dielectric constant shows a downward trend due to the influence of the ionic bond character of B-site ions and the B-site Rattling effect. The packing fraction is the main factor affecting the Q × f value of BZMNS ceramics. As the x value increases, the temperature coefficient of the resonant frequency ( τ f ) gradually decreases. Ion polarizability dilution is the primary mechanism for regulating τ f of BZMNS ceramics. The BZMNS ceramics sintered at 1480 °C with x = 0.3 exhibit optimal microwave dielectric properties ( ε r = 31.44, Q × f = 62,273 GHz (at f₀ = 6.5 GHz), τ f = + 2.48 ppm·°C −1 ), indicating that this ceramic has good application prospects.
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