FOOD CHEMISTRY
·
2022
·
IF 9.231
Xiang Li,Chang-Ling Sun,Yu Xu,Shi-Hui Shan,Han Zheng,Xiao-Lu Guo,Jiang-Ning Hu
A novel method for benzo(α)pyrene (Bαp) enrichment from an oil matrix was developed by using magnetic nanoparticles (Fe 3 O 4 @dopamine/graphene oxide, Fe 3 O 4 @DA/GO) as extraction absorbents, and the chemical properties of the synthesized nanoparticles were characterized. Various parameters were investigated to optimize the extraction of Bαp from oils. Under optimal conditions (pH, 4; extraction time, 0.5 min; elution solvent, 1 mL; absorbent weight, 20 mg; elution time, 0.5 min), these nanoparticles showed excellent abilities to enrich Bαp from the saponified oil solution and were easily separated by a magnet. High-performance liquid chromatography plus fluorescence detection (HPLC-FLD) was then applied to determine the Bαp content with excellent linearity (R 2 = 0.999). The detection limit was 0.13 µg/kg, while the limit of quantification was 0.42 µg/kg. The spiked recoveries of Bαp in oils ranged from 73.5% to 121%. Compared with previous reports, the proposed method displayed many advantages, including a high efficiency of oil matrix removal, short extraction time, and convenient extraction procedure.
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Journal of the American Chemical Society
·
2022
·
IF 16.383
Qian Zhang,Yong-Hui Song,Jing-Ming Hao,Yi-Feng Lan,Li-Zhe Feng,Xue-Chen Ru,Jing-Jing Wang,Kuang-Hui Song,Jun-Nan Yang,Tian Chen,Hong-Bin Yao
All-inorganic CsPbI3 perovskite is attractive for deep-red light-emitting diodes (LEDs) because of its excellent carrier mobility, high color purity, and solution processability. However, the high phase transition energy barrier of optically active CsPbI3 black phase hinders the fabrication of efficient and bright LEDs. Here, we report a novel α-BaF2 nanoparticle substrate-promoted solution-processable heteroepitaxial growth to overcome this hindrance and obtain high-quality optically active γ-CsPbI3 thin films, achieving efficient and bright deep-red LEDs. We unravel that the highly exposed planes on the α-BaF2 nanoparticle-based heteroepitaxial growth substrate have a 99.5% lattice matching degree with the (110) planes of γ-CsPbI3. This ultrahigh lattice matching degree initiates solution-processed interfacial strain-free epitaxial growth of low-defect and highly oriented γ-CsPbI3 thin films on the substrate. The obtained γ-CsPbI3 thin films are uniform, smooth, and highly luminescent, based on which we fabricate efficient and bright deep-red LEDs with a high peak external quantum efficiency of 14.1% and a record luminance of 1325 cd m–2.
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Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Ni Yun,Lv Zhixiang,Wang Zhou,Kang Shouyu,He Dawei,Liu Ruijiang
Magnetic α-Fe2O3/Fe3O4 heterostructure nanosheets were fabricated via hydrothermal calcination. The activity of penicillin G acylase (PGA), which was covalently immobilized onto silica-decorated heterostructure nanosheets, achieved the highest activity of 387.03 IU/g after 18 h of incubation with 0.1 ml of PGA. In contrast, the activity of free PGA reached the highest level when the temperature was 45°C with a pH of 8.0. However, the activity of free PGA changed more dramatically than immobilized PGA as the relative conditions changed. Moreover, the Michaelis–Menten constant (Km) and reusability of immobilized PGA were also explored. The results showed that free PGA Km and maximum rate (Vmax) were 0.0274 M and 1.167 μl/min, respectively. Km and Vmax values of immobilized PGA were 0.1082 M and 1.294 μl/min, respectively. After 12 cycles of repetitive use, immobilized PGA remained approximately 66% of its initial activity, indicating that the PGA immobilized onto the heterostructure nanosheets showed better stability and reusability than free PGA.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Xiaoxiao Li,Tong Jiang,Yu Wang,Jingjing Dong,Zhengyu Jin,Yuxiang Bai
Limosilactobacillus reuteri 121 4,6-α-glucanotransferase (GtfBΔN) modifies starch by cleaving (α1 → 4) linkages and introducing non-branched (α1 → 6) linkages to produce functional starch derivatives. Research has mainly focused on GtfBΔN converting amylose (linear substrate), whereas the conversion of amylopectin (branched substrate) has not been studied in detail. In this study, we used GtfBΔN to understand amylopectin modification and performed a set of experiments to analyze this modification pattern. The donor substrates were segments from the non-reducing ends to the nearest branch point in amylopectin as shown from the results of the chain length distribution of GtfBΔN-modified starches. Decreased and increased contents of β-limit dextrin and reducing sugars, respectively, during the incubation of β-limit dextrin with GtfBΔN indicated that the segments from the reducing end to the nearest branch point in amylopectin act as donor substrates. Dextranase was involved in the hydrolysis of the GtfBΔN conversion products of three different substrates groups, maltohexaose (G6), amylopectin, and G6 plus amylopectin. No reducing sugars were detected, therefore, amylopectin was not used as an acceptor substrate, and no non-branched (α1 → 6) linkages were introduced into it. Thus, these methods provide a reasonable and effective approach to studying GtfB-like 4,6-α-glucanotransferase in analyzing the roles and contribution of branched substrates.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Gaoyin Xiong,Lisha Ma,Han Zhang,Yanping Li,Wanshuang Zou,Xiaofan Wang,Quansheng Xu,Jingteng Xiong,Yanping Hu,Xiaoyin Wang
In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively. Their physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects were investigated and compared. Physico-chemical analysis showed that they were acidic heteropolysaccharides, which had α- and β-configurations. ERP-W, ERP-AK and ERP-W-AK were mainly composed of Rha, Ara, Gal, Glc and Gal-UA, while ERP-AC and ERP-W-AC were dominantly made up of Rha, Gal and Gal-UA. ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight. Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O 2 − radicals scavenging activities, Fe 2+ chelating ability and α-glucosidase inhibitory effects. Moreover, these actions were considerably related to their physicochemical properties especially monosaccharide composition and molecular weight. Therefore, polysaccharides extracted by water and alkaline solvents from Evodiae fructus could be developed as promising natural antioxidants and α-glucosidase inhibitors in the food and medicine industries.
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PHYTOCHEMISTRY
·
2021
·
IF 4.072
Bing-Rui Liu,Hai-Rong Zheng,Xian-Jun Jiang,Pu-Zhao Zhang,Guo-Zhu Wei
Phytochemical investigation of Lycopodium cernuum L. afforded seven undescribed serratene triterpenoids named 3 β, 21 β- dihydroxyserra-14-en-24-oic acid-3 β- (5′-hydroxybenzoate) ( 1 ), 3 β, 21 β , 24-trihydroxyserrat-14-en-3 β -(5′-hydroxyl benzoate) ( 2 ), 3 β , 14 α , 15 α , 21 β -tetrahydroxyserratane-24-methyl ester ( 3 ), 3 β , 14 α , 21 β -trihydroxyserratane-15 α -(4′-methoxy-5′-hydroxybenzoate)-24-methyl ester ( 4 ), 3 β , 14 α , 21 β -trihydroxyserratane-15 α -(4′-methoxy-5′-hydroxybenzoate) ( 5 ), 3 β -hydroxy-21 β -acetate-16-oxoserrat-14-en-24-oic acid ( 6 ), 3 β , 21 β -dihydroxy-16 α , 29-epoxyserrat-14-en-24-methyl ester ( 7 ), together with eleven known compounds ( 8 – 18 ), whose chemical structures were elucidated through spectroscopic analysis of HRESIMS, 1D NMR , 2D NMR and comparison between the literature. All compounds were evaluated for their α -glucosidase inhibitory activity for the first time. The results showed that compounds 1 , 2 , 4 , 5 , 6 , 10 , 13 , 15 , and 16 were among the most potent α-glucosidase inhibitors, with IC 50 values ranging from 23.22 ± 0.64 to 50.65 ± 0.82 μM. Structure – activity relationship (SAR) studies indicated that the combined properties of the 5-hydroxybenzoate moiety at C-3, β -OH at C-21, COOH- at C-24, and Δ 14,15 groups enabled an increase in the α-glucosidase inhibitory effect. In addition, molecular docking studies showed that the potential inhibitors mainly interact with key amino acid residues in the active site of α -glucosidase through hydrogen bonds and hydrophobic forces .
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Tongtong Ren,Suli Liu,Hong Li,Angui Zhang,Xingang Li,Wei Yuan,Xin Gao
Utilization of F-T synthetic to produce value-added C 8 linear α-olefin is a promising alternative approach, however, the purification of α-olefin from F-T synthetic products is a great challenge, especially for removing 2-methyl-1-heptene (MH). This article addresses the novel method of etherification reaction to remove MH from F-T synthetic products, and develop by far the most efficiency reactive distillation (RD) method to intensified the etherification process. The scarcity of thermodynamic data was measured and regressed by the activity coefficient models, furthermore, kinetics of the etherification reaction of MH with methanol was explored using an ion exchange resin as catalyst, and then fitted by a pseudo-homogeneous model. The pilot-scale RD experimental results show that the conversion of MH reaches 91.83 % and the purity of α-olefin increases by 14.9 wt% (percentage points). Process analysis method was used to realize the optimization of design and operating parameters of etherification RD column based on the developed rigorous model. The optimization results show that the conversion of MH achieves 99.74 % and the purity of α-olefin achieves 96.87 wt%, which is also illustrated that the novel method for removing MH is proposed paving the way for purification of α-olefin from F-T synthetic products.
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ACS Sensors
·
2022
·
IF 9.618
Mei Zhao,Limei Luo,Yongxian Guo,Binglu Zhao,Xiangfeng Chen,Xingang Shi,Mashooq Khan,Jin-Ming Lin,Qiongzheng Hu
α-Amylase (AMS) in human serum is a critical biomarker for the early diagnosis of pancreatic damage. In addition, the inhibition of α-amylase has long been thought to decrease the occurrence of diabetes. Thus, it is critical to construct a facile and convenient method for the determination of AMS and its inhibitor. In this study, we demonstrate a novel amylase sensor based on translating the viscosity change of the aqueous solution into the difference of the water diffusion length on a pH paper strip. AMS can be quantitatively detected by measuring the viscosity change of the amylopectin solution in the presence of AMS with different concentrations. The paper-based AMS sensor has a very high sensitivity with a detection limit of 0.017 U/mL and also shows excellent specificity. In addition, the inhibitory effect of acarbose on AMS is demonstrated with the IC50 value determined to be 21.66 ± 1.13 μg/mL. Furthermore, it is also evaluated for the detection of AMS in human serum samples of healthy people and acute pancreatitis patients. The difference in amylase levels between the two groups is unambiguously distinguished. Overall, this study provides a very simple, cost-effective, equipment-free, high-throughput, and label-free method for rapid and quantitative detection of α-amylase and may have significant applications in the diagnosis of acute pancreatitis and the screening of AMS inhibitors.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Mingqian Wang,Xia Liu,Zeyuan Yu,Jia Ran,Fujian Chen,Yang Li,Xiaodong Yu,Qiyi He
Pumpkin seed protein (PSP) is a promising precursor of bioactive peptides, but the mechanisms underlying the α-glucosidase inhibitory activity of its hydrolysates are not yet fully understood. In the present work, a previously unreported tetrapeptide with α-glucosidase inhibitory activity, YYPW, was obtained from PSP through simulated gastrointestinal digestion and in silico screening. Kinetic assays demonstrated that YYPW is a potent mixed-type inhibitor (IC 50 = 279.61 μmol/L). Molecular docking revealed that YYPW engages both the catalytic dyad (Asp443/Asp542) and peripheral aromatic residues via π–π stacking and hydrogen bonding. Subsequent molecular dynamics simulations indicated that this dual-site binding induces structural compaction and reduces the conformational flexibility of key catalytic loops. Multi-spectroscopic analyses (UV–Vis, fluorescence, and circular dichroism) confirmed a shift in microenvironmental polarity and a transition from α-helix to β-sheet. These findings help elucidate the inhibitory mechanism of YYPW and highlight its potential for further development in glycemic control applications.
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Autophagy
·
2026
·
IF 18.6
Hui Han,Sheng Wang,Jiajia Lu,Jing Li,Jinlong Zheng,Yongchuan Li,Hongtao Lu,Aimin Chen
Sacral plexus transection (SPT), mostly caused by high‑energy trauma, induces secondary death of spinal motor neurons in the lumbosacral segments and contributes to poor clinical outcomes after nerve repair. This study aimed to investigate the protective effect of α‑ketoglutarate (AKG) against ferroptosis in spinal motor neurons following SPT and the underlying molecular mechanism. Our results showed that endogenous AKG levels were significantly decreased in the spinal cord of SPT rats and in oxidative stress‑injured motor neurons, accompanied by abnormal expression of core ferroptosis‑related proteins, including downregulated glutathione peroxidase 4 and solute carrier family 7 member 11, upregulated acyl-CoA synthetase long-chain family member 4, as well as Fe2+ overload, malondialdehyde accumulation, and glutathione depletion. Exogenous AKG supplementation markedly reversed these anomalies, suppressed ferroptosis, and improved neuronal survival. Mechanistically, AKG specifically enhanced O-GlcNAcylation at the Thr177 site of PTEN‑induced kinase 1 (PINK1) and suppressed its ubiquitin-mediated degradation, which in turn selectively activated PINK1-PRKN-dependent mitophagy to eliminate damaged mitochondria and sustain mitochondrial homeostasis. Furthermore, downregulation of isocitrate dehydrogenase 1 (IDH1) after SPT was identified as a critical upstream event leading to endogenous AKG depletion. This study systematically elucidates the key role of the IDH1‑AKG‑PINK1 O‑GlcNAcylation ‑mitophagy axis in regulating SPT‑induced ferroptosis in spinal motor neurons, providing novel targets and experimental evidence for neuroprotective therapy of sacral plexus injury.
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JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
·
2023
·
IF 3.4
Yao YuXuan,Zheng ShengLan,Chi ShiLin,Chen Feng,Cai Ning,Cai ZhenZhen,Li Zhipeng,Ni Hui
The off-flavor of Pichia pastoris strains is a negative characteristic of proteins overexpressed with this yeast. In the present study, the P. pastoris GS115 overexpressing a α-L-rhamnosidase was taken as the example to characterize the off-flavor via sensory evaluation, gas chromatography-mass spectrometer (GC-MS), gas chromatography-olfaction (GC-O), and omission test. The result showed the off-flavor was attributed to the strong sweaty note, moderate metallic and plastic notes. Four volatile compounds, i.e. tetramethylpyrazine, 2,4-di-tert-butylphenol, isovaleric acid, and 2-methyl butyric acid were identified to be major contributors to sweaty note. Dodecanol and 2-acetylbutyrolactone were identified to be contributors to the metallic and plastic notes, respectively. It is the first study on the off-flavor of P. pastoris strains, helping understand metabolites with off-flavor of this yeast. Interestingly, it is the first study illustrating 2-acetylbutyrolactone and dodecanol with plastic and metallic notes, providing new information about the aromatic contributors of biological products.
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Foods
·
2023
·Vol. 12
·期 22
·4142
·
IF 5.2
Kumaravel Kaliaperumal,Linyan Zhang,Liangliang Gao,Qin Xiong,Yan Liang,Yueming Jiang,Jun Zhang
The α-glucosidase inhibitor is of interest to researchers due to its association with type-II diabetes treatment by suppressing postprandial hyperglycemia. Hesperidin is a major flavonoid in orange fruit with diverse biological properties. This paper evaluates the effects of hesperidin on α-glucosidase through inhibitory kinetics, fluorescence quenching, and molecular docking methods for the first time. The inhibition kinetic analysis shows that hesperidin reversibly inhibited the α-glucosidase activity with an IC50value of 18.52 μM and the inhibition was performed in an uncompetitive type. The fluorescence quenching studies indicate that the intrinsic fluorescence of α-glucosidase was quenched via a static quenching process and only one binding site was present between the hesperidin and α-glucosidase. The interaction between them was spontaneous and mainly driven by hydrogen bonds, as well as hydrophobic forces. Furthermore, the molecular docking results suggest that hesperidin might bond to the entrance or outlet part of the active site of α-glucosidase through a network of five hydrogen bonds formed between hesperidin and the four amino acid residues (Trp709, Arg422, Asn424, and Arg467) of α-glucosidase and the hydrophobic effects. These results provide new insight into the inhibitory mechanisms of hesperidin on α-glucosidase, supporting the potential application of a hesperidin-rich orange product as a hypoglycemic functional food.Keywords:hesperidin;α-glucosidase;antidiabetic;flavonoid;orange
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危险品化学品经营许可证（带存储）