FOOD CHEMISTRY
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2025
·
IF 9.8
Hong Wu,Yumin Hao,Xinyan Cheng,Huiping Wang,Wenjuan Dong,Shaomin Shuang,Qin Hu,Chuan Dong,Xiaojuan Gong
Nitrite (NO 2 − ) is common in food and water and poses health risks, requiring rapid detection. Here, a ratiometric fluorescent probe combining phenylenediamine-derived carbon dots (Ph-CDs) with Rhodamine B (RhB) was developed for quick, accurate NO 2 − sensing. Under acidic conditions, NO 2 − reacts with amino groups on Ph-CDs via diazotization, causing a clear fluorescence change within 200 s. RhB provides a stable reference, allowing self-calibrating dual-emission output that reduces matrix and environmental interferences. Coupled with smartphone imaging, the probe enables real-time visual detection and quantification without complex instruments. The system showed high sensitivity (LOD: 0.10 μM) and good accuracy (recoveries: 96.8 %–101.8 %) when tested in food and tap water, demonstrating strong reliability in complex samples like cured meats. This portable, user-friendly platform offers an effective tool for on-site NO 2 − screening, supporting food safety and environmental monitoring, especially in resource-limited settings.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Jiawen Niu,Fawang Zhang,Chenlu Liu,Kunrong Xie,Yuanxiang Zhang,Jie Zhang,Xijun Wang,Chengchao Song,Boyuan Li,Yuhang Jiang,Tianlong Li,Yufu Wang
Regulating microglia to modulate the inflammatory response in the early stages of spinal cord injury is crucial for neural repair. Commonly used drugs to inhibit inflammation and microglial activity in clinical practice, such as glucocorticoids and immunosuppressants, are associated with potential side effects. Eleutheroside B (EB), a natural plant extract, has been demonstrated an efficient anti-inflammatory action with low toxicity and the ability to promote neural repair and axon regeneration, suggesting its potential role in treating SCI. Recently, magnetically driven microrobots have demonstrated the ability to deliver drugs and provide precise targeting in deep tissues, which may help increase the dose of EB at the injury site. In this study, we design biomimetic magnetically driven microrobots loaded with EB, which offer efficient motion control and drug delivery capabilities. In a mouse SCI model, the magnetic microrobot with macrophage membrane functionalized and EBs (MPE robot) actively target the injured area using rotating magnetic fields in the early stages of injury, modulated the local microglial phenotype to a neuroprotective state, inhibited local inflammation, and promote axon regeneration and neurological recovery. This approach demonstrates that a biomimetic microrobot loaded with EB offers an effective strategy for treating SCI and other central nervous system diseases.
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PHYTOMEDICINE
·
2024
·
IF 6.7
Jie Yao,Haoqiang Wan,Jingmei Zhang,Wanying Shen,Xiaofang Wei,Chenyan Shi,Baoru Ou,Dongyu Liu,Lanlan Ge,Jia Fei,Xiaobin Zeng
Background Studies have shown that phenylethanoid glycosides (PhGs) have multiple pharmacological effects such as anti-inflammatory, hepatoprotective or neuroprotective functions, whereas their anti-tumor effects are rarely studied. Tubuloside B (Tub B) is a PhG isolated from Cistanche deserticola , a traditional Chinese medicine. To date, there is a lack of comprehensive research regarding the biological activity of Tub B. Purpose The subject of the current study was to investigate the anti-hepatocellular carcinoma (HCC) cell activity and the underlying mechanism of Tub B. Methods We evaluated the in vitro anti-migratory effect of Tub B by scratch and transwell assays. RNA-seq was employed to identify the differential genes by Tub B. Besides, the functional mechanism of Tub B was investigated by distinct molecular biology techniques including immunofluorescent staining, quantitative PCR, as well as western blot analysis. Subsequently, we utilized Hep3B cells for in vivo metastasis assays through spleen injection and evaluated the anti-migratory effect of Tub B in hepatocellular carcinoma (HCC). Results Tub B exhibited in vitro and in vivo inhibition of HCC cell migration. Tub B decreased the expression of transcriptional target genes downstream of the Hippo pathway, including CTGF, CYR61, and N-cadherin as determined by RNA-seq. Furthermore, mechanistic studies confirmed that Tub B increased phosphorylation of YAP at S127, which contributes to YAP cytoplasmic localization. Additionally, overexpression of YAP abrogated Tub B-induced inhibition of HCC migration and the mRNA levels of CTGF, CYR61, and N-cadherin . Conclusions Taken together, these results illustrated that Tub B demonstrated great potential in inhibiting migration of HCC, and a portion of its impact can be attributed to the modulation of the Hippo-YAP pathway.
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DYES AND PIGMENTS
·
2025
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IF 4.2
Ningxi Wu,Sirui Hang,Shuying Chen,Kai Wang,Yue Zheng
We report the design and synthesis of CatP, a sulfur-substituted hemicyanine-based activatable probe, for the sensitive detection of cathepsin B (CatB) activity. This probe was applied to study the dynamic changes of hepatic CatB in diabetic mellitus (DM), a prevalent metabolic disease. The probe incorporates a Val-Cit linker specifically cleaved by CatB, triggering an intramolecular charge transfer (ICT) mechanism that generates strong near-infrared (750 nm) fluorescence and photoacoustic signals. Structural optimization through sulfur substitution significantly enhanced photoacoustic signal intensity compared to conventional oxygen-substituted analogs. In diabetic mouse models, CatP revealed significantly elevated hepatic CatB activity, which was normalized by metformin treatment as confirmed by both in vivo imaging and ex vivo immunofluorescence. Comprehensive biosafety evaluation demonstrated excellent biocompatibility with >90 % cell viability and no observable organ toxicity. These findings establish sulfur-modified hemicyanine as a promising scaffold for developing high-performance activatable probes, and demonstrate the value of CatP as a functional dye for studying protease activity in biomedical research.
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INORGANIC CHEMISTRY COMMUNICATIONS
·
2025
·
IF 5.4
Denis P. Ordinartsev,Andrey A. Rempel,Xiaojun Han
Finding new, effective photocatalysts for the degradation of organic pollutants is a pressing issue in water treatment. Oxide photocatalysts are difficult to synthesize and impractical to use because they are most effective in the form of suspensions, sols, or nanoscale powders. This article demonstrates the photocatalytic ability of a hybrid polyvinyl alcohol-titanyl film photocatalyst to degrade Rhodamine B and formaldehyde. This hybrid material is an easily synthesized alternative to oxide photocatalysts that can be used to photodegrade organic pollutants. 99.8 % of Rhodamine B is degraded in 90 min with a catalyst-to-solution ratio of 1:100 for a 0.05 mM Rhodamine B solution. The film photocatalyst is transparent and colorless, with a band gap of 3.39 eV and an adsorption edge at 354 nm, corresponding to a forbidden direct transition. After irradiation, the photocatalyst's color changed to blue, and new photodegradation activity peaks were registered at 400 and 500 nm. The reversible color change of the photocatalyst enhances light absorption and promotes the effective separation of electron-hole pairs. Based on data from the Scavenger study and TOC measurements, a mechanism for the photodegradation of Rhodamine B on a hybrid polyvinyl alcohol-titanyl photocatalyst was proposed. The structure of the hybrid photocatalyst was characterized using XPS and IR spectroscopy, and the distribution of titanium in polymeric matrix was analyzed by SEM combined with EDX. This work demonstrates the potential of the synthesized polyvinyl alcohol-titanyl hybrid photocatalyst for the efficient photodegradation of organic pollutants.
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CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Shi-Xing Liu,Qian Wang,Li Jiang,Shang-Gao Liao,Qing-De Long,Xu Zhang,Xue-Long Yan
Phytochemistry investigation of Euphorbia helioscopia led to the isolation of 22 diterpenoids, including five lathyranes, 11 jatrophanes, and six other types. Among them, eupholine A and B ( 1 and 2 ) are a pair of previously undescribed C-6′ lathyrane epimers. The chemical structures of 1–22 were elucidated using spectral data analysis, and the absolute configurations of 1 together with another jatrophane diterpenoid ( 3 ) were determined by X-ray single-crystal diffraction techniques. Besides, the full nuclear magnetic resonance (NMR) assignments of 3 and 4 were first determined by analysis of their two-dimensional NMR data. All the isolates were screened for inhibition activity of fibrinogen on transforming growth factor-β1-stimulated LX-2 cells, and seven compounds (mainly lathyranes and jatrophanes types) showed certain inhibitory effects at 20 µM, being comparable to that of the positive control, silymarin. Notably, compound 14 (jatrophane type) showed significant inhibitory activity that is superior to silymarin. The study suggested that jatrophane diterpenoids and their analogs (lathyrane types) may serve as a potential chemical entity against liver fibrosis. Graphical
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LANGMUIR
·
2025
·
IF 3.9
Heng Li,Chengzhong Zhang,Yuhang Jiang,Wenwu Ni,Mingshuo Li,Jing Wang
Widespread dye contamination poses a serious threat to humans and aquatic environments. Metal–organic frameworks (MOFs) with abundant reactive groups and high specific surfaces are candidates for the adsorption of dyes. In this study, good growth of UiO-66-SO3H crystals on the surface of nylon-6 fibers was achieved based on the idea of in situ growth. UiO-66-SO3H with a sulfonic acid group was successfully introduced into nylon-6 electrostatic spinning fiber, and the UiO-66-SO3H@nylon-6 membrane with high specific surface area and a three-dimensional mesh structure was obtained, which effectively solved the problems of clogging of the equipment, difficulty in being separated and recovered from the water, the possible secondary pollution of the water environment caused by the powder form of the MOF material, etc. The UiO-66-SO3H@nylon-6 membrane has a good adsorption capacity for rhodamine B (RhB), and the experimental data are consistent with the pseudo-second-order kinetic model and Langmuir model, which shows that the adsorption is a spontaneous heat absorption process. Pore-filling, hydrogen bonding, π–π interactions, and electrostatic attraction induced by –SO3H are the adsorption mechanisms of RhB on this adsorbent. In conclusion, the UiO-66-SO3H@nylon-6 membrane is an adsorbent capable of rapid adsorption of RhB and can be easily separated from water for recovery, regeneration, and utilization. This study may provide ideas for the application of MOFs in the field of pollutant removal.
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JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
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2025
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IF 6.2
Riyi Lin,Ruiqi Liu,Lijuan Wang,Yiya Wang,Ningmin Zhu,Dengyu Chen,Liqiang Zhang
B-doping walnut shell char was prepared by the premodified doping method. The effects of B-doping amount, doping temperature, and coupling activation agent (activator amount, activation temperature) on the physicochemical structure and CO 2 adsorption performance of walnut shell biochar were investigated using BET, SEM, Raman, XRD, FT-IR, and XPS characterization techniques. The results indicated that B-doping walnut shell char exhibited a high specific surface area, and the pore structure was dominated by micropores. As the amount of B-doping increased, the CO 2 adsorption capacity of walnut shell char initially rose and then declined. The optimal doping mass ratio was 2:1, and the best pyrolysis temperature was 800 °C. The biochar pores became more abundant and disorderly after KOH activation. Excessive amounts of activators could damage the pore structure of activated char, thereby reducing the adsorption performance. The optimal mass ratio of the activator to walnut shell char was 1:1. As the activation temperature increased, the adsorption capacity of activated char for CO 2 initially improved and subsequently declined. The optimal activation temperature of walnut shell char was 700 °C. The optimal CO 2 adsorption performance was achieved by the CK1–1–700, which was 76.70 % higher compared to W800 without B-doping. Additionally, CK1–1–700 exhibited good cyclic performance. B-doping and activation treatment significantly enhanced the CO 2 adsorption capacity of walnut shell char. These research findings could serve as a reference for the high-value utilization of biomass.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
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IF 6.953
Wenchao Zhang,Weiyi Wang,Xin Liu,Ying Chen,Kang Ouyang,Zuzhang Wei,Huan Liu,Weijian Huang
Porcine astrovirus (PAstV) is prevalent in pigs worldwide and could cause clinical symptoms such as diarrhea and encephalitis. The capsid protein (Cap) of PAstV plays a determinant role for virus immunological characteristics. In this study, the major antigenic regions of PAstV1 Cap were expressed through prokaryotic expression systems and immunized to BALB/c mice. Finally, two anti-Cap monoclonal antibodies (named mAb F4-4 and D3F10) were screened by indirect immune-fluorescence assay (IFA). A series of truncated GST-fused or artificially synthesized peptides were used to detect their reactivity with the mAbs and PAstV positive serum. Two novel B cell epitopes ( 120- GNNTFG -125 , 485- RISDPTWFSA -494 ) were identified by using these two mAbs. Moreover, sequence alignment result showed that epitope 120- GNNTFG -125 was highly conserved in type 1 PAstV capsid protein. Cross-reactivity analysis further confirmed the genotype-specificity of mAb F4-4. The results of this study demonstrated to be the first description of monoclonal antibody preparation and B-cell epitope mapping on PAstV capsid protein, which may provide new information on the biological significance of PAstV capsid protein and lay a foundation for the development of PAstV immunological tests and genotype diagnostic methods.
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Molecular Medicine Reports
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2021
·Vol. 24
·期 5
·1-11
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IF 2.952
Shuang Zhang,Sihan Lin,Qiaofei Tang,Zhiyong Yan
Allergic rhinitis (AR) is an IgE‑mediated upper airway disease with a high worldwide prevalence. MicroRNA (miR)‑205‑5p upregulation has been observed in AR; however, its role is poorly understood. The aim of the present study was to investigate the effect of miR‑205‑5p on AR‑associated inflammation. To establish an AR model, BALB/c mice were sensitized using an intraperitoneal injection of ovalbumin (OVA) on days 0, 7 and 14, followed by intranasal challenge with OVA on days 21‑27. A lentiviral sponge for miR‑205‑5p was used to downregulate miR‑205‑5p in vivo via intranasal administration on days 20‑26. Reverse transcription‑quantitative PCR revealed that miR‑205‑5p was upregulated in AR mice. Notably, miR‑205‑5p knockdown reduced the frequency of nose‑rubbing and sneezing, and attenuated pathological alterations in the nasal mucosa. The levels of total and OVA‑specific IgE, cytokines IL‑4, IL‑5 and IL‑13, and inflammatory cells, were decreased by miR‑205‑5p knockdown in AR mice. In addition, miR‑205‑5p knockdown inhibited nucleotide‑binding oligomerization domain‑like receptor family pyrin domain‑containing 3 (NLRP3) inflammasome activation by reducing the expression levels of NLRP3, apoptosisassociated specklike protein containing a CARD, cleaved caspase‑1 and IL‑1β by western blot analysis. B‑cell lymphoma 6 (BCL6) was confirmed as a target of miR‑205‑5p by luciferase reporter assay. In conclusion, the present findings suggested that miR‑205‑5p knockdown may attenuate the inflammatory response in AR by targeting BCL6, which may be a potential therapeutic target for AR.
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Biomolecules
·
2025
·Vol. 15
·期 12
·1694
·
IF 4.8
Siyu Huang,Xiaorui Li,Xin Lu,Biao Kan
Polymyxins, including polymyxin B (PMB), are last-resort antibiotics against multidrug-resistant Gram-negative infections in humans and livestock. Residual polymyxins from wastewater and manure can accumulate in soil, facilitating the emergence and spread of polymyxin resistance.Paenibacillus polymyxa, a natural polymyxin producer used in crop cultivation, may increase soil polymyxin burden. Since PMB strongly adsorbs to soil, its reliable quantification has been challenging. To address this, the extraction solvent and solid-phase extraction procedure were optimized to improve recovery and reduce matrix effects. We developed and validated a UPLC-MS/MS method to quantify PMB in soil. The method showed linearity (10–1000 ng/g), with a limit of detection of 0.86 ng/g and a limit of quantification of 2.12 ng/g. Method validation confirmed acceptable analytical performance. A 28-day monitoring of PMB in soil inoculated with varyingP. polymyxadoses revealed a dose-dependent increase over the first 14 days, followed by a decline; PMB remained detectable on day 28. Ecological risk assessment using the risk quotient (RQ) indicated that PMB levels in the high-dose group (2 × 108CFU/100 g) approached the high-risk threshold (RQ ≥ 1) on day 14, while lower doses posed low to medium risk. This work provides a soil PMB quantification method and insight into the ecological risk ofP. polymyxaapplication.
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Journal of Ocean University of China
·
2025
·
IF 1.2
Jing Qianqian,Cheng Yajie,Wang Wenhui,Yin Junna,Wei Meiyan,Xu Tongyi,Shao Changlun
Mycobacterium marinum can cause disease in both human beings and fishes. Infected fish exhibits symptoms commonly referred to as fish tuberculosis. In this research, we screened a 14-Membered Resorcylic Acid Lactones (RALs) library constructed in the laboratory for in vitro anti- Mycobacterium marinum activity and found that several compounds, including compounds 3 , 4 , 6 , 10 , and 17 – 26 , showed good activity. Notably, the trace natural product cochilimycin B (compound 3 ) possesses significant long-lasting inhibitory function to Mycobacterium marinum . It also shows synergistic effects with drugs like isoniazid, ethambutol, and bedaquiline ( FICI ≤0.5). In addition, cochilimycin B showed a significant inhibitory effect on the biofilm produced by Mycobacterium marinum , with an inhibition rate of 65.9%. To solve the bottleneck problem of natural product drug sources, we realized an efficient one-step semi-synthetic method to obtain compound 3 with a yield of 32.1%. In vivo , inflammatory changes in BALB/c mice infected with Mycobacterium marinum were characterized. After 12 h of infection, IL-1 β was 20%–30% lower in the spleen and tail of the 3 -treated group compared to the control group. In the thymus and tail of the treated group, TNF- α levels were 30%–60% lower than in the control group. This is the first time that the significant anti- Mycobacterium marinum effect of cochilimycin B from 14-Membered Resorcylic Acid Lactones (14-RALs) was reported, offering a new chemical molecule for anti- Mycobacterium marinum drug research.
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