RSC Advances
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2019
·Vol. 9
·期 27
·15196-15204
·
IF 3.049
Yun Hong,Dongsen Mao,Rui Wu,Zhe Gao,Tingting Meng,Rongrong Wang,Lin Liu,Jing Miao
DNAzymes have the potential to suppress gene expression through sequence-specific mRNA cleavage and can therefore play an important role in various gene therapies. Hepatitis B virus (HBV) is still one of the most serious liver infections in people around the world and is difficult to treat. We previously designed a 10-23 DNAzyme called DrzBS, which targets HBV S gene expression, but this enzyme depends on exogenous delivery, and so its application has been limited. To overcome this limitation, we have now developed a chitosan-based nanocarrier (chitosan-g-stearic acid, CSO–SA) for intracellular delivery of DrzBS, then compared the inhibition effect of our CSO–SA/DrzBS complex to a common transfection reagent, Lipofectamine™ 2000/DrzBS, on hepatitis B surface antigen expression. The synthesized CSO–SA assembles into micelles in an aqueous solution and exhibits excellent cytoplasmic targeting, and could protect DrzBS from degradation by ribonuclease. CSO–SA/DrzBS showed a higher inhibition rate (IR) than Lipofectamine™ 2000/DrzBS. Moreover, at the same DrzBS concentration (1.2 μmol L−1), the maximum IR of CSO–SA/DrzBS micelles was 2.4-fold that of the Lipofectamine™ 2000/DrzBS complex, and held on for 96 hours. Compared with Lipofectamine™ 2000/DrzBS, CSO–SA/DrzBS achieved a higher HBV inhibition effect. This study demonstrates that CSO–SA micelles can serve as a potential vector for DrzBS and that CSO–SA/DrzBS micelles are a promising application for anti-HBV gene therapy.
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APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Jiayi Chen,Xinyan Xiao,Yi Wang,Zhihao Ye
A novel Ag nanoparticles (NPs) decorated WO 3 nanoplates/g-C 3 N 4 (CN) nanosheets 2D/2D heterostructure was successfully synthesized by a solvent evaporation and in situ calcination method. The as-prepared WO 3 /Ag/CN ternary composite exhibited remarkably enhanced photocatalytic activity towards rhodamine B (RhB) and tetracycline (TC) degradation compared with WO 3 , CN, Ag/WO 3 , Ag/CN and WO 3 /CN under visible light irradiation. The enhanced photocatalytic activity could be attributed to the efficient separation of photogenerated charge carriers, the strong redox ability and enhancement of visible-light absorption derived from the construction of Z-scheme 2D/2D heterostructure. Ag NPs not only possess the surface plasmon resonance (SPR) effect, but also act as an electron mediator in Z-scheme heterostructure. The UV–vis absorption spectra analysis indicated that the loading of Ag NPs can enhance the visible-light adsorption. And the decreased photoluminescence and the increased photocurrent demonstrated the high separation rate of photoinduced charge carriers owing to the successful heterojunction construction. Furthermore, the possible degradation pathways of RhB and TC were proposed according to HPLC-MS analysis. The results of radical trapping experiments indicated that O 2 − and OH were the primary active species during the photocatalytic degradation process.
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Journal of the Taiwan Institute of Chemical Engineers
·
2018
·
IF 3.849
Chunxue Li,Huinan Che,Chunbo Liu,Guangbo Che,Paul. A. Charpentier,William Z. Xu,Xiuyan Wang,Lihui Liu
In this study, we report a facile and green strategy to produce graphitic carbon nitride quantum dots (g-C 3 N 4 QDs)/BiVO 4 Z-scheme nanoheterostructure, which is composed by g-C 3 N 4 QDs assembled on the surface of mesh-like BiVO 4 crystals. As compared with pure BiVO 4 , the g-C 3 N 4 QDs/BiVO 4 composite showed significantly enhanced photocatalytic performance towards degradation of rhodamine B (RhB) and Tetracycline (TC) under visible light irradiation, which is attributed to the increased surface area, (increased number of photocatalytically active sites) and the improved efficiency of separation of photogenerated electron-hole pairs through an efficient charge transfer Z-scheme system. The stability and durability of photocatalyst are also discussed in detail. Moreover, the possible enhanced photocatalytic mechanism was put forwarded and explained by the electron spin resonance (ESR) spin-trap technique and active species trapping experiments. The successful implementation of this work will have guiding significance for the preparation of photocatalysts, and provide theoretical basis and experimental guidance for future development of Z-scheme photocatalytic mechanism of the hybrid photocatalysts with better performance.
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JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2018
·Vol. 165
·期 3
·B118
·
IF 3.662
Yuting Wu,Yun Yang,Wu Lei,Caiwei Li,Qingli Hao,Cheng Zhang,Yuehua Zhang,Juan Su
Ascorbic acid (AA) electrochemical sensor upon modification with porous g-C3N4(PCN)/Poly (3,4-ethylenedioxythiophene) (PEDOT) composite was constructed by a facile co-deposited method. The obtained PCN bearing more oxygen-containing functional groups demonstrates excellent dispersion. Transmission electron microscopy (TEM) and Raman spectroscopy were performed to evaluate the structure and morphology of PCN/PEDOT composite. Detailed electrochemical behaviors of AA were investigated by cyclic voltammetry (CV). Under the optimal experimental conditions, the oxidation peak current was linear with the concentration of AA in a wide range from 10 μM to 1500 μM. The combination of PCN and PEDOT brings out large electro-active area and facilitates the electron-transfer greatly, which makes PCN/PEDOT composite appear to be an excellent choice as modified material for the construction of AA sensor.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2018
·
IF 3.779
Shan Wang
A novel fluorescence probe was built by using graphitic carbon nitride (g-C 3 N 4 ) nanosheets . Fluorescence of the g-C 3 N 4 nanosheet solution was effectively quenched by Fe 3+ via photoinduced electron transfer (PET). Moreover, the linear detection range for Fe 3+ was 0–43.33 nM with a limit of detection of 4 nM. Fluorescence recovery of g-C 3 N 4 was observed upon addition of ascorbic acid (AA) to the g-C 3 N 4 nanosheet–Fe 3+ system, and the paramagnetic Fe 3+ was reduced to diamagnetic Fe 2+ by blocking PET. An “on–off–on” fluorescence response was observed through the oxidation–reduction between Fe 3+ and AA. Furthermore, a wide linear detection range for AA was found in 0–26.67 nM. Thus, this system can play a dual role in the selective and sensitive detection of Fe 3+ and AA in aqueous media via “on–off–on” fluorescence response.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2018
·
IF 3.862
Chenguang Yang,Wantong Song,Dawei Zhang,Haiyang Yu,Lei Yin,Na Shen,Mingxiao Deng,Zhaohui Tang,Jingkai Gu,Xuesi Chen
Gemcitabine is widely used for anticancer therapy. However, its short blood circulation time and poor stability greatly impair its application. To solve this problem, we prepared a poly ( l -glutamic acid)- g -methoxy poly (ethylene glycol)-gemcitabine conjugate ( l -Gem) with a 14.3 wt% drug-loading content. l -Gem showed concentration- and time-dependent cytotoxicity towards 4T1, LLC, MIA PaCa-2 and A2780 in vitro . Pharmacokinetic and biodistribution studies indicated that l -Gem had remarkably enhanced blood stability, prolonged blood circulation time and greatly improved selective tumor distribution compared with free gemcitabine. The area under the concentration–time curve from zero to infinity [AUC (0–∞) ] of l -Gem in plasma was 43-fold higher than that of free gemcitabine. The AUC (0–∞) of the inactive metabolite, 2′-deoxy-2′,2′-difluorouridine in the l -Gem group was ∼20% of that observed in the free gemcitabine group. The drug tumor accumulation ratio in the l -Gem group relative to the free gemcitabine group was 9.9 at 36 h, while the tumor AUC ratio was 15.8. Testing on Balb/C mice bearing the 4T1 tumor further demonstrated that l -Gem had significantly higher anticancer efficacy than free gemcitabine in vivo . These findings indicated that l -Gem has great potential for cancer treatment.
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CHINESE JOURNAL OF CATALYSIS
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2018
·
IF 3.525
Zhen Qin,Wenjian Fang,Junying Liu,Zhidong Wei,Zhi Jiang,Wenfeng Shangguan
A two-step photocatalytic water splitting system, termed a “Z-scheme system”, was achieved using Zn-doped g-C 3 N 4 for H 2 evolution and BiVO 4 for O 2 evolution with Fe 2+ /Fe 3+ as a shuttle redox mediator. H 2 and O 2 were evaluated simultaneously when the doping amount of zinc was 10%. Moreover, Zn-doped (10%) g-C 3 N 4 synthesized by an impregnation method showed superior active ability to form the Z-scheme with BiVO 4 than by in-situ synthesis. X-ray diffraction, UV-Vis spectroscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy were used to characterize the samples. It was determined that more Zn–N bonds could be formed on the surface of g-C 3 N 4 by impregnation, which could facilitate charge transfer.
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CARBOHYDRATE POLYMERS
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2018
·
IF 5.158
Muhammad Haroon,Li Wang,Haojie Yu,Raja Summe Ullah,Zain-ul-Abdin,Rizwan Ullah Khan,Qing Chen,Jian Liu
Carboxymethyl starch- g -polyvinylpyrolidones (CMS- g -PVPs) were prepared by grafting of carboxymethyl starch (CMS) with N -vinylpyrolidone (NVP) using different initiators. The grafting percentage of all the modified starches was determined and the modified starch (CMS- g -PVP-5) with maximum grafting percentage was characterized by elemental analysis, 1 H NMR and FT-IR spectroscopy. Crystallinity and thermal properties of CMS- g -PVP-5 were analysed by XRD and TGA, respectively. CMS- g -PVP-5 was then utilized for the adsorption of Rhodamine 6G (dye) from water under different p H, temperature, adsorbate doze and adsorbent concentration. This modified starch showed good adsorption ability towards Rhodamine 6G. CMS- g -PVP-5 was also applied for the adsorption of ammonia gas and proved an exciting adsorbent for ammonia.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2018
·
IF 2.891
Kedir Ebrahim Ahmed,Dong-Hau Kuo,Misganaw Alemu Zeleke,Osman Ahmed Zelekew,Angaw Kelemework Abay
Sn-WO 3 /g-C 3 N 4 composites were synthesized by calcination method. The materials were characterized with different techniques and photocatalytic activities examined by the degradation of anionic methyl orange (MO) and cationic Rhodamine B (RhB) under visible light. 8% Sn-WO 3 /g-C 3 N 4 showed superior degradation efficiency with 87% MO and 99% RhB dye removal within 120 and 50 min of irradiation time, respectively. X-ray photoelectron spectroscopy (XPS) analysis reveals molecular oxygen adsorption on the surface of the as-prepared composite material. The introduction of tin in Sn-WO 3 solid solution with a high atomic Sn:W ratio of 1:2 and the construction of interfacial hetrojunction with graphitic carbon nitride (g-C 3 N 4 ) plays a vital role to enhance the photocatalytic activity of the as-prepared composites by activating oxygen to react with dye molecules.
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INFRARED PHYSICS & TECHNOLOGY
·
2018
·
IF 1.851
Honghan Bu,Guoyue Xu,Chuyang Liu,Tengchao Guo,Shujuan Tan
The green BCG/MgCl 2 /PEG-g-CDA powders were synthesized at different temperatures via liquid-phase reaction method. The microstructures of the synthesized powders were analyzed and characterized by FT-IR, XRD and ChemDraw software. The effects of synthesized temperatures on infrared emissivity properties of the powders in the wavelength range 3–5 μm were investigated systematically. It is found that the powders heated at 100 °C exhibit the optimum crystallinity at room temperature and thus show the largest variation (0.4) in infrared emissivity with the test temperature varying from 50 °C to 65 °C. Furthermore, the mechanisms for the self-regulated infrared emissivity properties are also explored thoroughly. The variation of infrared emissivity with temperature is ascribed to the changes in resistivity, which is induced by the transformation of PEG-g-CDA between crystalline and amorphous as well as the molecular structure transition of BCG between quinone and lactone. Our results suggest that BCG/MgCl 2 /PEG-g-CDA powders are potential candidates for intelligent infrared camouflage.
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ANALYST
·
2018
·Vol. 143
·期 20
·5030-5037
·
IF 3.864
Qiong Wu,Fengxia Zhang,Huijuan Li,Zhihua Li,Qi Kang,Dazhong Shen
Herein, we report a ratiometric photoelectrochemical (PEC) immunosensor coupled with secondary antibodies–Co3O4 nanoparticle conjugates (Ab2–Co3O4 NPs) for signal amplification. The Ab1-g-C3N4@TiO2 NTs and BSA-g-C3N4@TiO2 NTs act as the sensing and reference photoelectrodes, where Ab1, BSA and TiO2 NTs are the capture antibodies, bovine serum albumin and TiO2 nanotube arrays, respectively. In the presence of target Ag, the sensing PEC photocurrent decreases due to the steric hindrance of Ab1 molecules, which increases the inner resistance of the photoelectrode. The signal-off response is enhanced further using Ab2–Co3O4 NP conjugates due to their steric hindrance effect and the consumption of electron donors by the Co3O4 NPs. Furthermore, the performance of the ratiometric PEC platform is tested using alpha-fetoprotein (AFP) as a model antigen. Under the optimal conditions, the concentration of AFP is detected in the range of 0.4 pg mL−1 to 40 ng mL−1 with a detection limit of 0.2 pg mL−1. This ratiometric strategy is beneficial to improve the reliability and anti-interference ability of PEC immunosensors.
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CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Jianhui Zhao,Ning Li,Ruixin Yu,Zhiwei Zhao,Jun Nan
It is difficult to achieve concurrent removal of nitrate (NO 3 − -N) and ammonia (NH 4 + -N) from water thoroughly by photocatalysis due to their opposite charges and inappropriate potentials of photo-induced carriers. Herein, an innovative magnetic field enhanced 3D/2D Mn 2 O 3 /g-C 3 N 4 photocatalytic system was developed for denitrification from NO 3 − -N and NH 4 + -N contaminated water under visible light. High removal efficiency of 94.5% and 97.4% for NO 3 − -N and NH 4 + -N were achieved after imposing magnetic field. Impressively, the extra magnetic field integrated the advantages of providing driving force for NO 3 − -N and NH 4 + -N approaching catalyst surface as well as facilitating separation of photo-induced charge carriers in newly-formed II type heterojunction by generating Lorentz force . Interestingly, the electrons on conduction band of g-C 3 N 4 could reduce NO 3 − -N to NO 2 − -N, N 2 and NH 4 + -N. Subsequently, the produced NO 2 − -N could be reduced to N 2 and NH 4 + -N. Meanwhile, the holes on valence band of Mn 2 O 3 could convert NH 4 + -N to N 2 selectively, which made the dominate contribution to high N 2 recovery (93.2%) during photocatalysis. Moreover, the reusable Mn 2 O 3 /g-C 3 N 4 achieved efficient denitrification under magnetic assisted photocatalysis in real water backgrounds such as tap water and river water. This study provided an efficient and promising strategy for nitrogen remediation from water.
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