FOOD HYDROCOLLOIDS
·
2026
·
IF 13.8
Mingrui Chen,Yuan Zhou,Xi Wei,Xiaojing Shen,Weijie Lan,Qing Zhang,Yaowen Liu
To efficiently recover rhamnogalacturonan I (RG-I) entrapped within the recalcitrant apple cell wall matrix, the present study established a two-step strategy combining polyol-based deep eutectic solvent (DES)/cellulase (CE) pretreatment and extraction using water. The highest CE activity was achieved in choline chloride (ChCl)/propylene glycol (PG) at molar ratio of 1:2 with water content of 23.57 wt% among twenty polyol-based DESs tested. The Kamlet-Taft parameters (α and β) of hydrated ChCl/PG showed positive correlations with CE activity (r = 0.85 for α, 0.88 for β), whereas viscosity and polarity (π∗) exhibited negative correlations (r = −0.56 for viscosity, −0.73 for π∗). Extraction using the ChCl/PG/CE-assisted water method yielded the highest recovery (17.8%), significantly exceeding those achieved with CE alone, ChCl/PG/CE alone or ChCl/PG-assisted water extraction. Compared with extracted control pectin (E-P, ChCl/PG-P), ChCl/PG&E-P contained the highest RG-I content with more/longer neutral sugar side chains (RG-I = 81.28 mol%, (Ara + Gal)/Rha = 4.50). Efficient hydrolysis of amorphous cellulose, coupled with enhanced interactions between ChCl/PG and exposed RG-I, synergistically promoted the dissociation of RG-I from cellulose-RG-I matrix and its subsequent dissolution in water. Finally, ChCl/PG&E-P was capable of forming stable gel in low concentration and a small amount of Ca 2+ . Due to the higher content of side chains, ChCl/PG&E-P gel exhibited higher gel strength and rheological moduli (G′ and G″) across varying pectin/Ca 2+ concentrations and pH conditions. This work offers a novel strategy for the efficient recovery of RG-I-enriched pectin from apple cell wall, which exhibits good characteristics as a gelling agent.
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高影响因子
DRUG DELIVERY
·
2017
·
IF 6.402
Hongmei Yan,Jie Song,Xiaobin Jia,Zhenhai Zhang
Baohuoside I is an effective but a poorly soluble antitumor drug. In this study, we prepared baohuoside I-loaded mixed micelles with didecyldimethylammonium bromide (DDAB) and d-a-tocopheryl polyethylene glycol succinate (TPGS) (DTBM) and active targeting mixed micelles (HDTBM) with hyaluronic acid (HA) as the targeting ligand on the surface of the mixed micelles. We performed a systematic comparative evaluation of the antiproliferative effect, cellular uptake, antitumor efficacy, and in vivo tumor targeting of these micelles using A549 cells. HDTBM showed improved cellular uptake and had a greater hypersensitizing effect on A549 cell lines than baohuoside I; half-maximal inhibitory concentration (IC50) was 8.86 versus 20.42 μg/mL, respectively. Results of the antitumor efficacy study and the imaging study for in vivo targeting showed that the mixed-micelle formulation had higher antitumor efficacy and achieved effective and targeted drug delivery. Therefore, our results indicate that HA/baohuoside I-M may be used as a potential antitumor formulation.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Huixiang Yan,Xiaodi Tang,Xudong Zhu,Yanbo Zeng,Xing Lu,Zhengzhi Yin,Yixia Lu,Yiwen Yang,Lei Li
Herein we report a sandwich-type electrochemical immunosensor for sensitive detection of cardiac troponin I (cTnI) using a new composite of carboxyl-terminated ionic liquid and helical carbon nanotubes (CIL-HCNTs) as platform and ferrocenecarboxylic acid (Fc-COOH) as signal label. The composite of CIL-HCNTs was prepared by a simple and convenient one-step ultrasound method and was then characterized by Fourier transform infrared spectroscopy, scanning electron microscope, cyclic voltammetry and chronocoulometry. CIL-HCNTs, possessing large surface area and high electrical conductivity, provide a good platform for anti-cTnI monoclonal antibody immobilization through the carboxyl groups of CIL-HCNTs. The Fc-COOH, as a signal label based on the oxidation process, was combined with the CIL-HCNT platform to construct the electrochemical immunosensor for cTnI determination. Under optimal conditions, the electrochemical immunosensor exhibited desirable performance for cTnI determination with a wide range of linearity from 0.01 to 60.0 ng mL −1 and a relatively low detection limit of 0.006 ng mL −1 . Importantly, the immunosensor showed excellent specificity and acceptable reproducibility as well as satisfactory reliability for human serum sample analysis.
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高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Yao Wang,Yuhan Liu,Yutong Sha,Jia Li,Luyao Han,Huili Chen,Shengdi Su,Ning Li,Xijing Chen,Di Zhao
A chiral UPLC-MS/MS method was developed and validated to determine oxiracetam enantiomers in human plasma, urine, and feces. The R-Oxiracetam and S-Oxiracetam were quantified using a CHIRALPAK ®AD3 column at 25 ℃, and the resolution was greater than 3.2. The S-Oxiracetam is the eutome r that isresponsible for the treatment of various brain damage. Isocratic elution was conducted at a flow rate of 0.9 mL/min for 6 min using the mixture of methanol and acetonitrile (methanol:acetonitrile, 15:85) containing 0.3‰ formic acid . The methods showed linearity at the range of 0.5–100 µg/mL for each oxiracetam enantiomer. A comprehensive validation process was carried out, covering aspects including linearity, selectivity, carryover, accuracy, precision, interferences, matrix effect, recovery, dilution integrity and stability in matrix and solution. The validated methods were successfully applied to quantifying R-Oxiracetam and S-Oxiracetam in human plasma, urine, and feces of 12 healthy subjects treated with either a single dose of 2 g S-Oxiracetam injection or 4 g Oxiracetam injection in a phase-I clinical trial . There was no significant difference for plasma pharmacokinetic parameters of S-Oxiracetam between the two regimens ( P＞0.05 ). The S-Oxiracetam and Oxiracetam were primarily eliminated through urine in their original form, with cumulative excretion rates of 92.16% and 85.92%, respectively, within 24 h after administration. Enantiomers interconversion was not observed in the plasma, urine, or feces. The results of this study suggest that replacing 4 g Oxiracetam injection with 2 g S-Oxiracetam injection could offer clinical benefits by lowering the dosage and mitigating potential risks, based on the pharmacokinetic characteristics.
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高影响因子
Materials Today Energy
·
2023
·
IF 9.3
Tianxiang Yang,Sining Yun,Chen Wang,Yongwei Zhang,Jiaoe Dang,Guangping Yang,Zan Gao,Dan Qiao,Mengmeng Zhang
Designing cost-effective counter electrode (CE) catalysts with advanced nanostructures and exploring their application in different dye-electrolyte systems is crucial for the development of dye-sensitized solar cells (DSSCs). In this work, two types of 3D network bio-based porous carbon (3D-BPC) loaded 0D niobium-based bimetallic oxide nanohybrids (CoNb 2 O 6 /3D-BPC and NiNb 2 O 6 /3D-BPC) were synthesized using a hierarchical strategy. Benefiting from the optimization of the electron transport pathways and enrichment of catalytically active sites via the hierarchical 0D/3D structure, formed by 3D-BPC and MNb 2 O 6 (M = Co, Ni), the catalytic reduction ability of MNb 2 O 6 /3D-BPC was significantly enhanced. To explore the photovoltaic performance of the MNb 2 O 6 /3D-BPC based DSSC, the I 3 − /I − electrolyte and N719 dye system and the Cu 2+ /Cu + electrolyte and Y123 dye system were simultaneously employed. The solar cells assembled with the CoNb 2 O 6 /3D-BPC and NiNb 2 O 6 /3D-BPC CE exhibited device efficiencies of 7.03% and 7.14% in the N719-I 3 − /I − system, respectively, and 1.84% and 2.42% in the Y123-Cu 2+ /Cu + system, respectively, which were almost equal to those of Pt under the same system. Additionally, MNb 2 O 6 /3D-BPC catalysts exhibited promising stability in I 3 − /I − electrolyte. This work provides guidance for the construction of low-cost and high-efficiency nanohybrid catalysts for new energy conversion devices.
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高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2018
·Vol. 16
·期 1
·1-10
·
IF 5.294
Wang Shuai,Qu Fei,Han Wenli,You Jinmao
Background Differentiation of telomere length is of vital importance because telomere length is closely related with several deadly diseases such as cancer. Additionally, G-quadruplex and i-motif formation in telomeric DNA have been shown to act as a negative regulator of telomere elongation by telomerase in vivo and are considered as an attractive drug target for cancer chemotherapy. Results In this assay, Ag nanoclusters templated by hyperbranched polyethyleneimine (PEI–Ag NCs) are designed as a new novel resonance Rayleigh scattering (RRS) probe for sensitive differentiation of telomere length and monitoring special motifs (G-quadruplex and i-motif). In this assay, free PEI–Ag NC probe or DNA sequence alone emits low intensities of RRS, while the formation of PEI–Ag NCs/DNA complexes yields greatly enhanced RRS signals; however, when PEI–Ag NCs react with G-quadruplex or i-motif, the intensities of RRS exhibit slight changes. At the same concentration, the enhancement of RRS signal is directly proportional to the length of telomere, and the sensitivity of 64 bases is the highest with the linear range of 0.3–50 nM (limit of detection 0.12 nM). On the other hand, due to the conversion of telomere DNA molecules among multiple surrounding conditions, a DNA logic gate is developed on the basis of two chemical input signals (K + and H + ) and a change in RRS intensity as the output signal. Conclusion Our results indicate that PEI–Ag NCs can serve as a novel RRS probe to identify DNA length and monitor G-quadruplex/i-motif through the different increasing degrees of RRS intensity. Meanwhile, the novel attributes of the nanoprobe stand superior to those involving dyes or labeled DNA because of no chemical modification, low cost, green, and high efficiency.
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高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2025
·
IF 2.8
Yufeng Ni,Chenxin Ding,Minlu Cheng,Li Ding
The use of conventional opioids that act on μ-receptors is a recognized care agent for moderate-to-severe acute treatment. However, recent attention has shifted to a new class of μ-receptor agonists which can selectively activate the G-protein pathway and thus have better efficacy and fewer side effects. Recently, YZJ-4729 was developed as a new G protein selective μ-opioid receptor agonist. And for its clinical trial investigation usage, a rapid LC-MS/MS method was established for the concurrent measurement of YZJ-4729 and its major metabolite M10 in human plasma. A step involving the precipitation of proteins was used for plasma sample preparation. The chromatography separation was done on a Poroshell 120 EC-C18 analytical column (2.1 × 50 mm, 2.7-μm, Agilent). Gradient elution was performed with 5.0 mM ammonium acetate (NH 4 Ac) and 0.1 % formic acid (FA) water solution as the mobile phase A and pure acetonitrile (ACN) as the mobile phase B. Detection occurred in the mode of positive ion electrospray ionization through multiple reaction monitoring using deuterium YZJ-4729 (d6-YZJ-4729) as the internal standard. The ionic transitions used were YZJ-4729: m/z 409.3 → 244.2; d6-YZJ-4729: m/z 415.3 → 244.2; M10: m/z 425.3 → 260.2;). The method showed excellent linearity across the ranges of 0.500 to 500 ng/mL for YZJ-4729 and 0.0500 to 50.0 ng/mL for M10. The method was utilized to assess the plasma concentrations of YZJ-4729 and M10 in healthy volunteers after a 30-min intravenous infusion in the phase I clinical study, and the clinical pharmacokinetic profiles of YZJ-4729 and M10 were described.
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高影响因子
Acta Crystallographica Section E-Crystallographic Communications
·
2019
·Vol. 75
·期 12
·1914-1918
·
IF 0
Huang T.,Wang H.,Zhao J.
The title compound, [Fe(C44H24N8Cl4)(CN)2][K2(C18H36N2O6)2]·2C4H8O was synthesized and characterized by single-crystal X-ray diffraction as well as FTIR and UV–vis spectroscopy. The central FeII ion is coordinated by four pyrrole N atoms of the porphyrin core and two C atoms of the cyano groups in a slightly distorted octahedral coordination environment. The complex molecule crystallizes with two tetrahydrofuran solvent molecules, one of which was refined as disordered over two sets of sites with refined occupancies of 0.619 (5) and 0.381 (5). It has a distorted porphyrin core with mean absolute core-atom displacements Ca, Cb, Cm and Cav of 0.32 (3), 0.22 (3), 0.56 (2) and 0.37 (14) Å, respectively. The axial Fe—Ccyano bond lengths are 1.991 (2) and 1.988 (2) Å. The average Fe—Np (Np is a porphyrin N atom) bond length is 1.964 (10) Å. One of the O atoms and several C atoms of the 222 moiety [222 = 4,7,13,16,21,24-hexaoxa-1,10-diazabicyclo[8.8.8]hexacosane] were refined as disordered over two sets of sites with occupancy ratios of 0.739 (6):0.261 (6) and 0.832 (4):0.168 (4). Additional solvent molecules were found to be highly disordered and their contribution to the scattering was removed using the SQUEEZE procedure in PLATON [Spek (2015). Acta Cryst. C71, 9–18], which indicated a solvent cavity of volume 372 Å3 containing approximately 83 electrons. These solvent molecules are not considered in the given chemical formula and other crystal data.
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危险品化学品经营许可证（带存储）