PLANT CELL TISSUE AND ORGAN CULTURE
·
2026
·Vol. 165
·期 1
·27
·
IF 2.8
Tang Meihua,Lu Xinyi,Gan Yongdie,Ahmed Sher,Hu Zhan,Li Dong,Wang Shuai,Sun Ranfeng
Waltheria indica L. is a traditional officinal plant with many medicinal and insecticidal properties. This study established an in vitro propagation system for W. indica and evaluated the repellent activity of the compounds from in vitro-derived plants against the nematode Meloidogyne incognita . We optimized the medium for axillary bud proliferation and rooting of sterile shoots. Adventitious buds were induced from hypocotyls on Murashige and Skoog (MS) + 0.1 mg/L 6-benzyladenine (BA). MS + 1.0 mg/L 6-BA + 0.2 mg/L 1-napthaleneacetic acid (NAA) + 1.0 mg/L 2,4-dichlorophenoxyacetic acid was the best media for adventitious root induction from callus. An adventitious root suspension culture system was successfully induced and established in MS medium supplemented with 1.0 mg/L NAA, using a 1% inoculum size and a 30-day subculture interval. We isolated and identified antidesmone and trans-tiliroside from W. indica plants. They demonstrated repellent activity against J2 of M. incognita with chemotaxis index of 0.41 and 0.22, respectively, at a concentration of 10 mg/L. The relative content of antidesmone in adventitious roots cultured in liquid suspension was significantly higher than that in tissue-cultured plantlets. Exogenous supplementation of 0.05 mg/L 6-BA significantly improved the accumulation of trans-tiliroside synthesis in stems and leaves by reversing the inhibitory effect of tissue culture conditions. This study provides a theoretical basis and technical support for the large-scale propagation, germplasm improvement, and regulation of active secondary metabolite production in W. indica.
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Foods
·
2026
·Vol. 15
·期 8
·1316
·
IF 6
Limin Guo,Silong Jia,Linna Wang,Hesheng Wang,Qiyuan Feng,Xinyu Yang,Xi Lv,Yaqian Yang,Tian Li,Shaoying Zhang,Youwei Yu
Currently, the resource wastage and safety hazards caused by fruit and vegetable spoilage are becoming increasingly prominent. Developing green, efficient, and non-toxic novel preservation materials has emerged as a hot spot in fruit and vegetable research. Based on this, this study utilized pomegranate peel as a raw material to prepare spherical multifunctional carbon dots (P-CDs) with an average particle size of 1.98 ± 0.58 nm through a one-step hydrothermal reaction. Subsequently, P-CDs were co-incorporated with L-cysteine (L-Cys) into a polyvinyl alcohol (PVA) and chitosan (CS) matrix to construct a novel composite coating material with combined antibacterial, antioxidant, and preservation functions. Experimental results demonstrate that P-CDs exhibit outstanding antioxidant activity and antibacterial performance. Compared to PVA/CS film, the P-CDs/L-Cys/PVA/CS film exhibited a 6.55 MPa increase in tensile strength and significantly enhanced thermal stability. Furthermore, the incorporation of P-CDs and L-Cys markedly boosted the PVA/CS film’s antioxidant activity (97% for ABTS; 85.69% for DPPH), antibacterial performance, and ultraviolet (UV) shielding capability. Coating cherry tomatoes with the P-CDs/L-cysteine/PVA/CS composite extended their shelf life by 6 days. This composite coating material exhibits preliminary biocompatibility and eco-friendly properties, aligning with green sustainable development needs and offering a novel potential solution for food preservation technology, while its practical applicability to food safety requires further comprehensive verification.
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JOURNAL OF MODERN OPTICS
·
2026
·
IF 0.9
Shiyi Yang,Mingyu Wang,Xinyi Bai,Bo Su,Shengbo Zhang,Hailin Cui,Cunlin Zhang
Terahertz (THz) spectroscopy is an effective method for material analysis, as molecules exhibit unique fingerprint spectra in this band. L-carnitine, a vitamin-like nutrient widely used in weight-loss health foods and nutritional additives, was investigated. Using THz time-domain spectroscopy (THz-TDS) and microfluidic chips, we measured the solid and solution absorption spectra (0.3–2.5 THz) of Acetyl-L-Carnitine Hydrochloride (ALC-HCL) and L-Carnitine L-Tartrate (LCLT). Results showed distinct spectral characteristics despite structural similarities. Density functional theory simulations identified the molecular vibrations and origins of the absorption peaks. A correlation was observed between the solid and solution states. This research provides a reference for analyzing food nutritional additives.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2019
·Vol. 54
·期 10
·2854-2862
·
IF 2.281
Liu Aolu,Huang Baohua,Lei Lin,Lu Yu-Jing,Zhou Jin-Lin,Wong Wing-Leung
The rhamnosyl group of naringin dihydrochalcone, neohesperidin dihydrochalcone, naringin and hesperidin was selectively removed by enzymatic hydrolysis using an immobilised α-L-rhamnosidase. Monoglycosylated products, including trilobatin, hesperetin dihydrochalcone-7-O-glucoside, prunin and hesperetin-7-O-glucoside, were isolated and characterised by 1H and 13C NMR and ESI-MS. To optimise the enzymatic reaction conditions and its process costs, the hydrolysis of neohesperidin dihydrochalcone to produce trilobatin was selected as a model reaction. Using a ratio of neohesperidin dihydrochalcone: immobilised α-L-rhamnosidase equal to 1:0.6, the trilobatin yields was over 98%. The recycle of enzyme was also investigated, obtaining trilobatin with a yields of 80% even when the twentieth reaction cycle was conducted. Moreover, antiradical and antimicrobial activities of the obtained flavonoid monoglucosides were examined by DPPH, FRAP and ORAC methods, and compared with the efficacy of parental flavonoid glycosides and their aglycone. The results highlight that some of the obtained flavonoid monoglucosides show significant improvement in the antioxidant activity.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Jing Xu,Yuedan Wang,Changhui Li,Mufang Li,Xue Liu,Liyan Yang,Dong Wang,Yan Tan
A bimetallic ZnNi-MOF was grown on carbon fiber via a one-pot hydrothermal method. Compared with single-metal MOFs, the resulting material exhibited enhanced luminescence and electrochemical activity, which enabled the construction of a flexible dual-mode sensor for rapid fluorescence-electrochemical detection of L-cysteine. Electrochemical detection exhibited a linear range of 1 nM-10 μM with a limit of detection (LOD) of 0.017 nM, while fluorescence sensing gave a linear range of 1 μM-1 M with a LOD of 0.89 μM. Both modes showed good selectivity and stability. Flexible sensors were also prepared by impregnating filter paper and textiles, wet spinning, and hydrogel microspheres. By combining the sensor with mobile phone software that collects the RGB values of the fluorescence color change, visual detection of L-cysteine can be achieved. The sensor successfully determined L-cysteine in human sweat and milk, with recoveries of 93–114% and RSDs of 2.43–5.32%, demonstrating promising applications in intelligent wearable monitoring, precision medicine, and food safety.
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ACS Omega
·
2025
·
IF 4.3
Kaiyue Zuo,Naiyu Liu,Peng Zhou,Yingrui Wei,Jian Lin,Xinjie Zhu
Low-density lipoprotein receptor (LDLR), which serves as one of the most major entry receptors for many viruses in both human and mouse cells, plays a vital role in virus infection. However, there are no effective small molecules available to inhibit LDLR expression and exhibit antiviral effects. Here, we screened Bruceine A (BA), a natural product derived from the major constituents in Brucea javanica(L.) Merr. which inhibited vesicular stomatitis virus (VSV) infection in vitro. Mechanistically, BA blocked viral adsorption and internalization, facilitating antiviral effects through lysosome-mediated degradation of LDLR. Genetic knockdown of Ldlr exhibited strong antiviral effects. To the best of our knowledge, BA is the first LDLR-selective inhibitor, and our findings reveal that BA may serve as a potent and broad-spectrum virus entry inhibitor based on LDLR entry receptor.
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Food Bioscience
·
2025
·
IF 5.9
Xiaona Gao,Xinke Gao,Tengteng Wang,Rentai Song,Yanshen Li,Nada K. Alharbi,Ashwag Shami,Fahad Al-Asmari,Fakhria A. Al-Joufi,Yulin Zhu
Foods in our daily life are susceptible to contaminate by L. monocytogenes due to its high stress resistance and wide distribution, which poses great risks to food safety. Carvacrol is the natural compound that has been recognized as the safe additives and can be utilized safely by food industry. The current study aimed to study the inhibitory effect and mechanism of carvacrol against L. monocytogenes . The results showed that carvacrol treatment resulted in disruption of cell integrity of L. monocytogenes , further led to leakage of intracellular substances. In addition, carvacrol treatment inhibited the activity of topoisomerase and β-galactosidase, which in turn affected the gene duplication and energy utilization of L. monocytogenes . Based on transcriptome analysis, it was found that 126 differential expression genes (DEGs) were existed between carvacrol treated groups and control groups, which were highly enriched in transmembrane transporter activity and transporter activity. In addition, those DEGs were enriched in 4 metabolic pathways with obvious enrichment including phosphotransferase system, starch and sucrose metabolism, quorum sensing, alanine, aspartic acid and glutamic acid metabolism. Finally, carvacrol treatment can inhibit the secretion or activity of hemolysin in L. monocytogenes , which was consistent with the result in transcriptome analysis. In conclusion, carvacrol could be a potential antimicrobial substance to inhibit the contamination of L. monocytogenes in foods.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Tianjie Nie,Yue Gao,Ya Gao,Wenqian Wang,Ziqiang Wang,Huiying Zhang,Haitao Chen,Shuqi Wang,Baoguo Sun
Odor–induced saltiness enhancement has become an effective way of reducing salt consumption, as recommended for health purposes, without compromising consumer acceptability. In this study, the odorants in fried white mushrooms were investigated using solvent-assisted flavor evaporation, chromatography–mass spectrometry, and gas chromatography–olfactometry. A total of 40 odorants were identified, with 27 exhibiting relative odor activity values ≥0.1. Among them, 8 compounds were considered relating to saltiness by gas chromatography–olfactometry/associated taste analysis. Sensory analysis revealed that 2,5-dimethylpyrazine, 2-ethyl-3,5-dimethylpyrazine, 3-(methylthio)propanal, and 1-octen-3-one significantly enhanced the saltiness perception of 0.3 % NaCl solution ( p < 0.05). The electroencephalography confirmed compared with NaCl solution (0.3 %), the brain response energy values were increased significantly by adding 3-(methylthio)propanal and 1-octen-3-one ( p < 0.05). This research explored the possibilities of mushroom-derived odorants in enhancing perceived saltiness and provided methodological supports for odor-induced taste enhancement research.
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JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Chenxi Wu,Xiaojin Tian,Ziyan Song,Wang Rui,Hezhao Bai,Hailin Li,Yidan Wang,Wenzhi Yang,Fei Tian
Designing adsorbents with high sensitivity, superior adsorption capacity, molecular specificity, and batch-to-batch reproducibility remains a significant challenge in separation science. For the first time, we synthesized a functionalized magnetic metal-organic framework (Fe₃O₄@SiO₂@MIL-101@CLCS), to adsorb magnoflorine, hesperidin, nitidine chloride, and chelerythrine from Toddalia asiatica (L.) Lam via electrostatic interactions and π-π stacking. In order to systematically analyze the target components, this study established a magnetic solid-phase extraction coupled with high-performance liquid chromatography-diode array detection (MSPE-HPLC-DAD) method, and comprehensively optimized the extraction and elution conditions. After optimization, the adsorption rates of Fe₃O₄@SiO₂@MIL-101@CLCS on the above four active ingredients were ranged from 52.52% to 82.43%, and the elution rates were all higher than 96.40%. The adsorbent retained good adsorption capacity after 5 reuse cycles and had excellent recyclability. The method demonstrated excellent linearity (r² ≥ 0.999), with a limit of detection (LODs) of 0.17–0.92 μg/mL and a limit of quantification (LOQs) of 0.30–1.65 μg/mL, confirming high accuracy and precision. This approach not only enhances analytical efficiency in traditional Chinese medicine studies but also expands the potential of magnetic solid-phase extraction for natural product research.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jinmeng Chen,Yafan Cai,Zhiwei Guo,Zhi Wang,Xiaotian Ma,Mengying Liang,Xuan Xie,Wei Zhuang,Dong Liu,Chenjie Zhu,Yongkun Lv,Anqi Zhao,Shilei Wang,Jingliang Xu,Hanjie Ying
Developing environmentally friendly and low-toxicity pretreatment methods is essential for advancing sustainable biorefinery processes. This study introduced an alkali-ammonia pretreatment at high-solid loading combined with co-production of L-lactic (L-LA) and xylitol, achieving high-value utilization of corn stover (CS). Through a fed-batch strategy, the process achieved a cellulose conversion rate of 84.51 % and a hemicellulose conversion rate of 67.12 % at 30 % solid loading, while eliminating the need for energy-intensive drying and detoxification steps. The obtained hydrolysate was subsequently employed for efficient two-stage fermentation: (1) L-LA fermentation yielded 78.21 g/L with 97.87 % optical purity upon 2 % nitrogen supplementation, and (2) xylitol fermentation achieved 16.48 g/L with a 0.51 g/g xylose yield. Overall, this easily operated and low-toxicity pretreatment effectively disrupted the recalcitrant structure of CS, enhanced product formation, and demonstrated strong potential as a promising and sustainable biorefinery strategy.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Lu Zhao,Wenhe Zhang,Min Li,Qi Liu,Zhuobing Zhang,Xiao Gao,Bin Qin,Ikuro Abe,Xian Jia,Song You
l-Leucine dehydrogenase (LeuDH)-mediated direct asymmetric reduction amination of prochiral α-keto acids represents an ideal approach for the synthesis of l-phenylglycine and its derivatives. However, limited substrate acceptance hinders their applications. Herein, we systematically investigated the substrate acceptance of LeuDHs for benzoylformic acid and its monosubstituted derivatives, revealing the correlation between substrate structure and enzyme activity. Meanwhile, to efficiently augment the LeuDH overall catalytic activity toward monosubstituted benzoylformic acids, we reported a two-stage screening strategy using o-chlorobenzoylformic acid (2e) as the starting screening substrate. A superior mutant library with 10–127-fold enhanced catalytic efficiency toward ortho-(M2-1 (L40V/V294A) and M2-2 (E114V/V294G)) and meta- and para-(M2-4 (E114L/V294G)) substituted benzoylformic acids was generated, and following future backtracking analysis, mutant M2-3 (L40V/T134G) with further increased catalytic activity of meta-substituted substrates was obtained. Furthermore, gram-scale asymmetric synthesis of l-phenylglycine (3a), L-p-fluorophenylglycine (3d), and L-o-chlorophenylglycine (3e) was performed with high substrate loading (1 M) and space-time yields up to 1800, 2016, and 2208 g/L·day, respectively. This study provides efficient biocatalysts for the synthesis of l-phenylglycine and its derivatives and establishes a referable engineering workflow for the collective evolution of amino acid dehydrogenase against differently positioned substituted substrate panels.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Yuman Wang,Lei Zhou,Suyun Lin,Junbing Zhang,David Julian McClements,Shengfeng Peng,Wei Liu
C-phycocyanin (CPC) is a natural pigment-protein complex that is widely used for its vivid blue color and bioactive properties in the food, cosmetics, and biomedical fields. However, its widespread use was limited due to its poor photostability. This study aimed to improve the photostability of CPC and investigate the mechanism by forming non-covalent complexes with three organic acids with different antioxidant properties: l -ascorbic acid, cinnamic acid, and ferulic acid. Spectroscopic analysis showed that organic acid binding induced alterations in CPC's secondary structure, slightly reducing α-helix content while increasing β-sheet formation, without disrupting the tetrapyrrole chromophore. Computational docking analysis indicated that organic acids slightly changed the protein conformation by adjusting the spatial arrangement of amino acids surrounding the chromophore. This study shows organic acids improve CPC's photostability, aiding its use as a natural blue food pigment.
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