ISME Journal
·
2021
·Vol. 15
·期 11
·3239-3257
·
IF 10.302
Zhang Jiao,Wang Fei,Yuan Bo,Yang Lei,Yang Yi,Fang Qi,Kuhn Jens H.,Song Qisheng,Ye Gongyin
We identified a 9332-nucleotide-long novel picornaviral genome sequence in the transcriptome of an agriculturally important parasitoid wasp ( Pachycrepoideus vindemmiae (Rondani, 1875)). The genome of the novel virus, Rondani’s wasp virus 1 (RoWV-1), contains two long open reading frames encoding a nonstructural and a structural protein, respectively, and is 3’-polyadenylated. Phylogenetic analyses firmly place RoWV-1 into the dicistrovirid genus Cripavirus . We detected RoWV-1 in various tissues and life stages of the parasitoid wasp, with the highest virus load measured in the larval digestive tract. We demonstrate that RoWV-1 is transmitted horizontally from infected to uninfected wasps but not vertically to wasp offspring. Comparison of several important biological parameters between the infected and uninfected wasps indicates that RoWV-1 does not have obvious detrimental effects on wasps. We further demonstrate that RoWV-1 also infects Drosophila melanogaster (Meigen, 1830), the hosts of the pupal ectoparasitoid wasps, and thereby increases its pupal developmental duration and fecundity, but decreases the eclosion rate. Together, these results suggest that RoWV-1 may have a potential benefit to the wasp by increasing not only the number of potential wasp hosts but also the developmental time of the hosts to ensure proper development of wasp offspring.
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Journal of Food Measurement and Characterization
·
2021
·Vol. 15
·期 4
·3553-3560
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IF 2.431
Yue Jinping,Fan Jiahui,Li Yihan,Ren Hong
Protein is the most important characteristic component of honey. The types and content of proteins in different kinds of honey are imparity, but those in the same kind of honey are very similar. Capillary zone electrophoresis (CZE) method, used to detect proteins, was developed for authenticating of four mono-floral honeys (n = 45), including medlar honey, chaste honey, jujube honey and locust honey. Electrophoresis conditions including capillary inner diameter (50–70 μm), pH (2.5–9), the concentration of anionic surfactant (0.3–0.5 mmol L −1 ), injection time (3–7 s) and separation voltage (3–7 KV) were optimized. The results showed that the honey sample was highly stable and the detection limit was 10%, the relative standard deviation of retention time and peak area were lower than 1.40% and 1.76%, respectively. The CZE fingerprints were established on the basis of different protein characteristics in different mono-floral honeys combined with chemometric analysis. All 45 honeys could be correctly identified according to their floral origins. This method does not need to confirm the type of protein, and has the advantages of simple and fast verification of the authenticity of honey.
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PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
·
2021
·Vol. 20
·期 1
·153-160
·
IF 3.982
Chen Wei,Yan Yuyan,Han Renlu,Hu Jun,Hou Yafei,Tang Keqi
A flower-like nanostructured MnO 2 with near-infrared (NIR) light-triggered high photothermal conversion capability of 30% and reactive oxygen species (ROS) generation ability was successfully developed. Different from the reported MnO 2 nanomaterials those were used in the nanomedicine field for only relieving tumor hypoxia and/or imaging, the flower-like MnO 2 inherently acts as a competent agent for simultaneously enhanced photothermal and photodynamic therapy. Graphic abstract A flower-like nanostructured MnO2 with near-infrared (NIR) light triggered high photothermal conversion capability of 30% and reactive oxygen species (ROS) generation ability was successfully developed.
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Reaction Kinetics Mechanisms and Catalysis
·
2021
·Vol. 132
·期 1
·301-315
·
IF 2.081
Hu Yi-Ming,Zheng Wan-Bin,Jia Ai-Ping,Luo Meng-Fei,Tang Cen,Lu Ji-Qing
A series of Ir/BN catalysts with same Ir contents (2 wt%) were calcined at different temperatures and tested for gas phase selective hydrogenation of crotonaldehyde. It was found that the thermal treatment exerted significant impacts on the structural and electronic properties of the 2Ir/BN catalysts. The catalyst calcined at 500 °C (2Ir/BN-5) showed the best performance, with a steady state of conversion of ca. 21% and a crotyl alcohol selectivity of ca. 83% at reaction temperature of 80 °C. Kinetic analysis indicated that the enhanced activity on the 2Ir/BN-5 catalyst was related to its higher surface coverage of crotonaldehyde because of the presence of small Ir particles (with a mean diameter of 3.4 nm) compared to those in the catalyst calcined at 600 °C (2Ir/BN-6, with a mean diameter of 4.4 nm). Also, the surface of the Ir species in the 2Ir/BN-5 catalyst are more electron-enriched compared to that in the 2Ir/BN-6, which suppressed the adsorption of C=C bond and thus improves the selectivity to CROL.
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CELLULAR ONCOLOGY
·
2021
·Vol. 44
·期 3
·557-568
·
IF 6.73
Wang Wanyue,Li Xin,Xu Ye,Guo Weikang,Yu Hui,Zhang Lu,Wang Yaoxian,Chen Xiuwei
Purpose Cervical cancer remains a major cause of cancer-related death in women, especially in developing countries. Previously, we found that the acetylation levels of chloride intracellular channel 1 (CLIC1) at lysine 131 were increased in cervical cancer tissues using a label-free proteomics approach. The aim of this study was to further determine the role of CLIC1 expression and its acetylation in cervical cancer. Methods CLIC1 expression and its implications for the prognosis of cervical cancer were analyzed using primary patient samples and cells, and the Gene Expression Profiling Interactive Analysis (GEPIA) database (gepia.cancer-pku.cn). The effect of CLIC1 on cervical cancer cells was evaluated using Cell Counting Kit (CCK)-8, flow cytometry, scratch wound healing, transwell, Western blotting and co-immunoprecipitation (Co-IP) assays. In vivo tumor growth was assessed using mouse xenograft models. Results We found that CLIC1 expression was increased in cervical cancer tissues and cells and that patients with a high CLIC1 expression tended to have a shorter overall survival time. Knockdown of CLIC1 significantly reduced in vitro cervical cancer cell proliferation, migration and invasion, and in vivo tumorigenesis. At the molecular level, we found that nuclear factor kappa B (NF-κB) activity was positively regulated by CLIC1. Pyrrolidine dithiocarbamate (PDTC), an inhibitor of NF-κB, attenuated the tumor-promoting effect of CLIC1. Moreover, we found that CLIC1 acetylation at K131 was upregulated in cervical cancer cells, which stabilized CLIC1 by inhibiting its ubiquitynation. Substitution of K131 inhibited CLIC1 ubiquitynation and promoted in vitro cervical cancer cell proliferation, migration and invasion, and in vivo tumor growth. In addition, we found that acetyltransferase HAT1 was responsible for CLIC1 acetylation at K131. Conclusion Our data indicate that CLIC1 acts as a tumor promoter in cervical cancer, suggesting a potential treatment strategy for cervical cancer by regulating CLIC1 expression and/or acetylation.
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ISME Journal
·
2021
·Vol. 15
·期 6
·1844-1857
·
IF 10.302
Liu Qing,Li Wei,Liu Di,Li Lingyan,Li Jie,Lv Na,Liu Fei,Zhu Baoli,Zhou Yuguang,Xin Yuhua,Dong Xiuzhu
Bacteria that inhabit glaciers usually produce carotenoids. Here, we report that a group of zeaxanthin-producing glacial Flavobacterium exhibited light-promoted growth. Of the tested 47 strains, 45 showed increased growths but two died under illumination at 50 μmol photon m −2 s −1 . Light stimulation occurred mainly in either anoxic or nutrient-poor cultures, while the same levels of light promotion were found for that grown at 14 and 7 °C. Pigment assays identified overrepresentative zeaxanthin but trace retinal in the light promoted 45 strains, while flexirubin was exclusively in the light-lethal two. Genomic analysis revealed the gene cluster for zeaxanthin synthesis in the 45 strains, in which 37 strains also harbored the proteorhodopsin gene prd . Transcriptomic analysis found that light-induced expressions of both the zeaxanthin synthesis and proteorhodopsin genes. Whereas, deletion of the prd gene in one strain did not diminish light promotion, inhibition of zeaxanthin synthesis did. In comparison, no light promotion was determined in a glacier Cryobacterium luteum that produced a non-zeaxanthin-type carotenoid. Therefore, light stimulation on the glacial Flavobacterium is mostly likely related to zeaxanthin, which could provide better photoprotection and sustain membrane integrity for the organisms living in cold environments.
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JOURNAL OF FLUORESCENCE
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2021
·Vol. 31
·期 2
·509-516
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IF 2.217
Zuo Pengli,Zhang Jingyu,Zhou Yuqing,Xie Ningjie,Xiao Deli
Fluorescent carbon nanoparticles exhibit merits in terms of photochemical stability, functional modification flexibility and excellent biocompatibility. Currently, fluorescent carbon nanoparticles are often obtained by bottom-up or up-bottom strategies. So far, there has been no literature concerning spontaneous formation of fluorescent carbon nanoparticles. However, we have successfully found that fluorescent carbon nanoparticles can form spontaneously in the glutaraldehyde solution. Then further investigations were conducted on the storage time, pH and temperature, which could affect the fluorescence intensity of glutaraldehyde solution. The results indicate that the value of the fluorescence intensity will increase with the extension of the storage time. Moreover, the fluorescence mechanism of the glutaraldehyde solution was studied according to its properties and experiment results. Transmission electron microscopy was used to demonstrate nanoparticles in the glutaraldehyde solution. It’s assumed that such phenomenon is probably attributed to the conjugated structure resulting from the polymerization of glutaraldehyde and the quantum confinement effect owing to the nanoparticles formed by the aggregation of polymers. Therefore, the spontaneous fluorescence produced by glutaraldehyde solution provides a simple and environmentally-friendly way to prepare fluorescent carbon nanoparticles.
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BMC GENOMICS
·
2021
·Vol. 22
·期 1
·1-19
·
IF 3.969
Yu Peng,Zhou Li,Yang Wen-Tao,Miao Li-jun,Li Zhi,Zhang Xiao-Juan,Wang Yang,Gui Jian-Fang
Background Loaches of Cobitinae, widely distributed in Eurasian continent, have high economic, ornamental and scientific value. However, the phylogeny of Cobitinae fishes within genera or family level remains complex and controversial. Up to now, about 60 Cobitinae mitogenomes had been deposited in GenBank, but their integrated characteristics were not elaborated.Results In this study, we sequenced and analyzed the complete mitogenomes of a female Cobits macrostigma. Then we conducted a comparative mitogenome analysis and revealed the conserved and unique characteristics of 58 Cobitinae mitogenomes, including C. macrostigma. Cobitinae mitogenomes display highly conserved tRNA secondary structure, overlaps and non-coding intergenic spacers. In addition, distinct base compositions were observed among different genus and significantly negative linear correlation between AT% and AT-skew were found among Cobitinae, genus Cobitis and Pangio mitogenomes, respectively. A specific 3 bp insertion (GCA) in the atp8-atp6 overlap was identified as a unique feature of loaches, compared to other Cypriniformes fish. Additionally, all protein coding genes underwent a strong purifying selection. Phylogenetic analysis strongly supported the paraphyly of Cobitis and polyphyly of Misgurnus. The strict molecular clock predicted that Cobitinae might have split into northern and southern lineages in the late Eocene (42.11 Ma), furthermore, mtDNA introgression might occur (14.40 Ma) between ancestral species of Cobitis and ancestral species of Misgurnus.Conclusion sThe current study represents the first comparative mitogenomic and phylogenetic analyses within Cobitinae and provides new insights into the mitogenome features and evolution of fishes belonging to the cobitinae family.
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JOURNAL OF PEST SCIENCE
·
2020
·Vol. 94
·期 2
·463-472
·
IF 4.578
Xu Hao,Zhao Jiajia,Li Faqian,Yan Qi,Meng Ling,Li Baoping
Riptortus pedestris (Fab.) is an important pest on soybean plants in East Asia. Females lay eggs scattered not only on host plants, but also on non-host plants, probably for avoiding detection by natural enemies. Newly hatched nymphs can develop to the second instar without the need of food except water. Second instar nymphs, the first-feeding stage with a strong locomotion, need to find suitable feeding sites on host plants. The mechanism is still not well understood. Here, we found second instars were attracted by both male and female adults, but generally repelled by second and fifth instar nymphs, wherein their responses were little affected by food volatiles (soybean seeds or seedlings). Nymphs distinctly differed from adults in chemical composition of the whole-body extracts. 4-oxo-( E )-2-Hexenal was the major component in nymph’s extracts, but it was not found in adults, whereas some ester compounds, such as ( E )-2-hexenyl esters, were exclusively found in adults and accounted for the main part in their extracts. The three esters, ( E )-2-hexenyl ( Z )-3-hexenoate (E2-6:Z3Hx), ( E )-2-hexenyl ( E )-2-hexenoate (E2-6:E2Hx) and myristyl isobutyrate, were male specific and had been reported as an aggregation pheromone. Synthetic 4-oxo-( E )-2-hexenal strongly repelled second instars, while ( E )-2-hexenyl hexanoate emitted by both males and females attracted them. The main component of the aggregation pheromone, myristyl isobutyrate, also strongly attracted second instars. How the findings may improve the efficiency of chemical traps in fields was discussed.
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ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
·
2020
·Vol. 113
·期 10
·1467-1477
·
IF 2.674
Yin Yi-Rui,Sang Peng,Yang Feng-Li,Li Tao,Yang Run-Feng,Liu Hong-Yan,Luo Zong-Long,Li Wen-Jun,Yang Li-Quan
A β-glucosidase gene ( bsbgl1a ) from Bacillus sp. CGMCC 1.16541 was expressed in Escherichia coli BL21 and subsequently characterized. The amino acid sequence shared 83.64% identity with β-glucosidase (WP_066390903.1) from Fictibacillus phosphorivorans . The recombinant β-glucosidase (BsBgl1A) had a molecular weight of 52.2 kDa and could hydrolyze cellobiose, cellotriose, cellotetrose, p-nitrophenyl-β- d -glucopyranoside ( p NPG), and p-nitrophenyl-β- d -xylopyranoside ( p NPX). Optimal activity for BsBgl1A was recorded at 45 °C with a pH between 5.6 and 7.6, and 100% of its activity was maintained after a 24 h incubation between pH 4 and 9. Kinetic characterization revealed an enzymatic turnover (Kcat) of 616 ± 2 s −1 (with cellobiose) and 3.5 ± 0.1 s −1 (with p-nitrophenyl-β- d -glucopyranoside). Interestingly, the recombinant enzyme showed cupric ion (Cu 2+ ), sodium dodecyl sulfate (SDS) and alcohol tolerance at 10 mM for Cu 2+ and 10% for both SDS and alcohol. Additionally, BsBgl1A had high tolerance for glucose (Ki = 2095 mM), which is an extremely desirable feature for industrial applications. Following the addition of BsBgl1A (0.05 mg/ml) to a commercial cellulase reaction system, glucose yields from sugarcane bagasse increased 100% after 1 day at 45 °C. This work identifies a Cu 2+ , SDS, alcohol, and glucose tolerant GH1 β-glucosidase with potential applications in the hydrolysis of cellulose for the bioenergy industry.
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 8
·1-9
·
IF 6.232
Xu Shaomei,He Xu,Huang Yibing,Liu Xin,Zhao Lihe,Wang Xinghua,Sun Ying,Ma Pinyi,Song Daqian
A hydrothermal method has been employed to synthesize a green and one-pot carbon dots-based sensor for ratiometric monitoring and imaging lysosomal pH in living cells. The carbon dots were directly functionalized by abundant amino groups during synthesis and exhibited dual emission bands at 439 and 550 nm under single-wavelength excitation of 380 nm without any additional modification. In addition to its small size, the established sensor had good biocompatibility. Owing to its abundant amino groups and good hydrophilicity, the sensor is able to target lysosome with high Pearson’s colocalization coefficients (0.935 and 0.924) and responds to change of lysosomal pH in living cells. It also had excellent pH sensitivity and reversibility, and anti-interference capability, thus enabling sensing pH change in intracellular environment in real time, as demonstrated by successful monitoring of lysosomal pH changes during lysosomal alkalization, dexamethasone-induced stimulation, and stress in Michigan Cancer Foundation-7 cells (blue channel, excitation = 405 nm and emission = 419–459 nm bandpass; and yellow channel, excitation = 405 nm and emission = 530–570 nm bandpass).
Graphical abstract
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WATER AIR AND SOIL POLLUTION
·
2020
·Vol. 231
·期 8
·1-14
·
IF 1.9
Zhao Tianyu,Cheng Hao,Liang Yinna,Xiong Jianhua,Zhu Hongxiang,Wang Shuangfei,Liang Jiaxiang,Chen Guoning
Photocatalysis has emerged as a promising approach for treating environmental pollution. In this study, TiO 2 /sponge composites with good photocatalytic activity in visible light were prepared via a simple and efficient low-temperature process and applied to the degradation of 2,4,6-trichlorophenol (2,4,6-TCP) present in papermaking wastewater. The process conditions for preparing TiO 2 /sponge composites were optimized by varying the TiO 2 dosage, cellulose dosage, and surfactant concentration. Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) results showed that TiO 2 successfully adhered to the sponge surface and that the composites achieved a good recycling effect. Degradation occurred under visible light, and a degradation rate of 81% for 2,4,6-TCP with initial concentration of 20 mg/L was achieved in 4 h. The fragments were analyzed using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS), which revealed the formation of 2-hydroxyvaleric acid (2-HVA) as a degradation product; a possible degradation mechanism is proposed to interpret these findings. Visible-light photocatalysis shows high potential for the rapid and environmentally friendly destruction of organic pollutants in papermaking wastewater.
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危险品化学品经营许可证（带存储）