ACS Applied Materials & Interfaces
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2014
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IF 5.9
Xiaoyu Mu,Juan Qiao,Li Qi,Ying Liu,Huimin Ma
Developing facile and high-throughput methods for exploring pharmacological inhibitors of d-amino acid oxidase (DAAO) has triggered increasing interest. In this work, DAAO was immobilized on the magnetic nanoparticles, which were modified by a biocompatible reactive polymer, poly(glycidyl methacrylate) (PGMA) via an atom transfer radical polymerization technique. Interestingly, the enzyme immobilization process was greatly promoted with the assistance of a lithium perchlorate catalyst. Meanwhile, a new amino acid ionic liquid (AAIL) was successfully synthesized and employed as the efficient chiral ligand in a chiral ligand exchange capillary electrophoresis (CLE-CE) system for chiral separation of amino acids (AAs) and quantitation of methionine, which was selected as the substrate of DAAO. Then, the apparent Michaelis–Menten constants in the enzyme system were determined with the proposed CLE-CE method. The prepared DAAO-PGMA-Fe3O4 nanoparticles exhibited excellent reusability and good stability. Moreover, the enzyme reactor was successfully applied in screening DAAO inhibitors. These results demonstrated that the enzyme could be efficiently immobilized on the polymer-grafted magnetic nanoparticles and that the obtained enzyme reactor has great potential in screening enzyme inhibitors, further offering new insight into monitoring the relevant diseases.
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International Journal of Polymeric Materials and Polymeric Biomaterials
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2016
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IF 1.667
Zhaosheng Hou,Hao Zhang,Wenqiang Qu,Zhen Xu,Zheng Han
In the study, a new approach for synthesizing biomedical segmented polyurethanes (SPUs) based on polyethylene glycol (PEG, Mn = 800), poly(ε-caprolactone-co-D,L-lactide) (PCLA) and aliphatic diurethane diisocyanates with a long uniform hard segment was developed. By the chain extension of a mixture of PEG diol and PCLA diol with diurethane diisocyanates based on inexpensive 1,6-hexanediisocyanate, three SPUs (SPU-I, SPU-II, and SPU-III) with different contents of hydrophilic segments (PEG) and hard segments were obtained. The chemical structures of diurethane diisocyanates, PCLA and SPUs were confirmed by 1H NMR, 13C NMR, FT-IR, HR-TOF-MS, and GPC. The SPU films exhibited similar thermostability, indicating that the hard segment content marginally affects on the thermostability. From the results of differential scanning calorimetry, two glass transition temperatures were observed, suggesting the microphase separation of soft and hard segments. The SPU films exhibited satisfactory mechanical properties with ultimate stress of 17.4–22.3 MPa and strain at break of 890–1060%, and the initial modulus increased with the increasing content of hard segments. In vitro degradation studies indicated that the time of SPU films to become fragments was 22–33 days, and the degradation rate increased with the increasing content of hydrophilic segments in SPU. Hence, the degradation time of SPU films could be controlled by adjusting the PEG content in SPU, which made them good candidates for further biomedical applications. Studies of in vitro drug release were conducted using vitamin B1 as model drugs. Drug-loaded films exhibited a high initial release rate and matrix-controlled release for more than two weeks, thereby demonstrating a promising material for a long-acting controlled release system. Cytotoxicity test of film extracts and cell attachment on the film surface were conducted using L929 mouse fibroblasts, and the results indicated that the SPU films possess excellent cytocompatibility and cell adhesive ability.
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INTERNATIONAL IMMUNOPHARMACOLOGY
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2017
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IF 2.956
Ye Tian,Zheng Li,Bingyu Shen,Qiaoling Zhang,Haihua Feng
Morin , a bioactive flavonoid extracted from the bark of Moraceae plants and many medicinal herbs, has anti-inflammatory and antioxidative effects. In this research, we explored the protective effects of morin against lipopolysaccharide (LPS) and d -galactosamine (D-GalN) induced acute liver injury in mice. Mice were given an intraperitoneal injection of morin before LPS and D-GalN treatment and the HepG2 cells were only given morin to investigate its effects. The results showed that morin markedly inhibited the production of serum alanine transaminase (ALT), aspartate aminotransferase (AST), interleukin-6 (IL-6), tumor necrosis factor (TNF-α) and hepatic TNF-α, IL-6, and myeloperoxidase (MPO) induced by LPS/D-GalN. In order to evaluate morin effect in the future, we investigated the expression of nuclear factor E2 related factor 2 (Nrf2), nuclear factor-kappaB (NF-κB), toll like receptor 4 (TLR4) on liver injury. Taken together, these results suggested that morin could exert the anti-inflammatory and anti-oxidative effects against LPS/D-GalN-induced acute liver injury by activating Nrf2 signal pathways and inhibiting NF-κB activation.
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MOLECULES
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2025
·Vol. 30
·期 4
·912
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IF 4.6
Huiyi Shang,Juntan Wang,Bishan Guo,Haihua Zhu,Huijuan Li
Phospholipase D (PLD) plays a pivotal role in the biosynthesis of phosphatidylserine (PS), but its practical application is constrained by limitations in stability and reusability. In this study, we successfully fabricated the Fe3O4@SiO2–graphene oxide (GO) nanocomposite by chemical binding of Fe3O4@SiO2and GO. Subsequently, PLD was immobilized onto the nanocomposite via physical adsorption, with the aim of enhancing catalytic stability, reducing mass transfer resistance, and improving reusability. Under optimal conditions, the immobilization efficiency reached 84.4%, with a PLD loading capacity of 111.4 mg/gsupport. The optimal pH for PS production by immobilized PLD shifted from 6.0 to 6.5, while the optimal temperature increased from 45 °C to 50 °C. Notably, the immobilized PLD demonstrated a shorter reaction time and a higher PS yield, achieving a 95.4% yield within 90 min, compared to the free PLD (78.1% yield within 150 min), representing a 1.04-fold improvement in production efficiency. Furthermore, the immobilized PLD exhibited outstanding storage stability and thermal stability, along with remarkable reusability. Even after being reused for 10 cycles, the PS yield still stays as high as 78.3%. These findings strongly suggest that the Fe3O4@SiO2–GO immobilized PLD has the potential for the efficient production of PS.
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APPLIED CATALYSIS B-ENVIRONMENTAL
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2025
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IF 21.1
Lizhang Chen,Xuan Wang,Tingyu Lu,Huan Pang,Songtao Zhang,Lin Xu,Gaixiu Yang,Qixing Zhou,Yawen Tang
As one of the most competitive alternatives to precious Pt-based electrocatalysts towards the oxygen reduction reaction (ORR), Fe-N-C single atom catalysts (SACs) are still eagerly anticipated to undergo further performance enhancement to advance their practical applications in diverse renewable energy systems. The regulation of energy level of Fe site through the manipulation of d-orbital splitting manner provides a novel route to improve the intrinsic activity, yet still in its infancy. We herein present a Fe d-orbital splitting modulation strategy via crafting atomically dispersed Fe-N 5 moieties with axial N ligand anchored on “lotus root”-like N-doped multichannel carbon nanofibers (abbreviated as FeN 5 @NMCNFs hereafter). Symmetrical experimental and theoretical findings reveal that near-range coordinated effect of axial N ligand results in the deformation of local symmetry, deep valence band spin-polarization of d orbitals and downshifted d-band center of Fe center site, endowing the optimized adsorption energetics of ORR intermediates and reduced reaction energy barrier. Accordingly, the as-developed FeN 5 @NMCNFs deliver Pt-competitive ORR activity and extraordinary long-term durability in KOH electrolyte with a halfwave potential of 0.87 V, exceeding a majority of recently reported non-precious SACs. Furthermore, when incorporated into liquid and flexible all-solid-state Zn air batteries (ZABs), the FeN 5 @NMCNFs||RuO 2 -assembled ZABs demonstrate superior electrochemical performance to the state-of-the-art Pt/C||RuO 2 counterpart. This work offers a new perspective for the performance optimization of SACs via the manipulation of the local coordination manner and the precise spin-polarization regulation strategy.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
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IF 8.1
Yuanchuan Ren,Yan Hu,Nanqi Ren,Xiaojuan Su,Fenghui Wu,Dandan Chen,Xuejun Zhu,Qiang Niu,Yan Guo
Persulfate (PMS) is an excellent free radical initiator with selective degradation of organic pollutants. This article presents the preparation of a high-performance Cu-SA@NCA(Single atom copper modified nitrogen doped aerogel carbon) electrocatalyst with adjustable interfacial separation enhancement effect for the first time. Under the optimal process parameters, the removal rates of ENO, COD, and TOC obtained after 5 min of reaction were 100 %, 73.41 % and 55.15 %, respectively. The average energy consumption was saved by 59.63∼123.25 kWh/kg ENO. After 6 cycles of degrading ENO wastewater, the removal rate of ENO only decreased by 8.55 %. It was demonstrated through characterization such as high-angle annular dark field-scanning transmission electron microscopy (HAADF-STEM), X-ray absorption near edge structure (XANES), and Fourier Transform Extended X-ray Absorption Fine Spectroscopy (FT-EXAFS) that the single atom copper loaded on the Cu-SA@NCA surface is tightly bound to N in NCA through Cu-N 4 bond bridges, promoting the formation of double electron centers on the catalyst surface. The particle electrode catalyst significantly enhances the PMS activation process over a wide pH range and can accelerate the degradation of enoxacin (ENO). As the reaction center for PMS adsorption and activation, Cu-N 4 has a lower charge transfer resistance and can attract PMS to the catalyst surface, resulting in a higher current response in the PMS system. Organic pollutants release electrons through the carbon matrix of carbon based catalysts and transfer them to Cu-SA@NCA-PMS, promoting PMS activation and the formation of high valence copper ions, enhancing the degradation of pollutants. Density functional theory (DFT) further indicate that the regulatory effect of N on the electron distribution of Cu d orbitals is beneficial for the stretching and cleavage of Cu active centers by PMS, accelerating the process of SO 4 − · and ·OH generation rate, which is consistent with the results of XANES. In addition, during the degradation process of salt containing organic pollutants in PMS, Cu-SA@NCA exhibits strong resistance to inorganic ions, natural organic matter, and pH value. This work developed a new catalyst that selectively produces SO 4 − · and ·OH with 97.46 % selectivity, achieving high selectivity and efficiency in degrading pollutants.
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EUROPEAN JOURNAL OF PHARMACOLOGY
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2024
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IF 4.2
Yufang Ma,Zhenming Ma,Yiyuan Zhang,Chunyun Luo,Puxin Huang,Jing Tong,Hong Ding,Honghui Liu
Apigenin and baicalein are structurally related flavonoids that have been reported to have multiple pharmacological activities. The aim of this study was to investigate the protective effects and potential mechanisms of apigenin and baicalein in D-galactose-induced aging rats. First, apigenin and baicalein showed remarkable antioxidant activity and anti-glycation activity in vitro. Secondly, the protective effects of apigenin and baicalein on aging rats were investigated. We found that apigenin and baicalein supplementation significantly ameliorated aging-related changes such as declines in the spatial learning and memory and histopathological damage of the hippocampus and thoracic aorta. In addition, our data showed that apigenin and baicalein alleviated oxidative stress as illustrated by decreasing MDA level, increasing SOD activity and GSH level. Further data showed that they significantly reduced the accumulation of advanced glycation end products (AGEs), inhibited the expression of RAGE, down-regulated phosphorylated nuclear factor (p–NF–κB (p65)). Our results suggested that the protective effects of apigenin and baicalein on aging rats were at least partially related to the inhibition of AGEs/RAGE/NF-κB pathway and the improvement of oxidative damage. Overall, apigenin and baicalein showed almost equal anti-aging efficacy. Our results provided an experimental basis for the application of apigenin and baicalein to delay the aging process.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Jinhong Gao,Lingli Zhang,Dongxin Zhao,Xin Lu,Qiang Sun,Heng Du,Hongyan Yang,Kui Lu
Aspergillus oryzae β-D-galactosidase (β-Gal) efficiently hydrolyzes sesaminol triglucoside into sesaminol, which has higher biological activity . However, β-Gal is difficult to be separate from the reaction mixture and limited by stability. To resolve these problems, β-Gal was immobilized on amino-functionalized magnetic nanoparticles mesoporous silica pre-activated with glutaraldehyde (Fe 3 O 4 @mSiO 2 -β-Gal), which was used for the first time to prepare sesaminol. Under the optimal conditions, the immobilization yield and recovered activity of β-Gal were 57.9 ± 0.3 % and 46.5 ± 0.9 %, and the enzymatic loading was 843 ± 21 U enzyme /g support . The construction of Fe 3 O 4 @mSiO 2 -β-Gal was confirmed by various characterization methods, and the results indicated it was suitable for heterogeneous enzyme-catalyzed reactions. Fe 3 O 4 @mSiO 2 -β-Gal was readily separable under magnetic action and displayed improved activity in extreme pH and temperature conditions. After 45 days of storage at 4 °C, the activity of Fe 3 O 4 @mSiO 2 -β-Gal remained at 92.3 ± 2.8 %, which was 1.29 times than that of free enzyme, and its activity remained above 85 % after 10 cycles. Fe 3 O 4 @mSiO 2 -β-Gal displayed higher affinity and catalytic efficiency. The half-life was 1.41 longer than free enzymes at 55.0 °C. Fe 3 O 4 @mSiO 2 -β-Gal was employed as a catalyst to prepare sesaminol, achieving a 96.7 % conversion yield of sesaminol. The excellent stability and catalytic efficiency provide broad benefits and potential for biocatalytic industry applications.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2024
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IF 20.2
Shanpeng Li,Yuling Zhuo,Dong Liu,Hui Pan,Zhenbo Wang
Improving the Pt atoms utilization efficiency on self-supported electrodes is crucial for industrial seawater hydrogen production , but effective strategies are still lacking. Herein, we reported a novel strategy to anchor highly surface-exposed Pt single atoms on Ni 3 S 2 /Co 9 S 8 with abundant S vacancies (Pt–Ni 3 S 2 /Co 9 S 8 –Sv) as a self-supported electrode. Physical characterizations and theoretical calculations confirm that the strong Pt–S electron bridges with the coordinating role of surface S vacancies triggers the d -orbital electron rearrangements and regulates the local electron structures between the Co/Ni and Pt sites. Notably, the Pt–Ni 3 S 2 /Co 9 S 8 –Sv electrode displays an ultralow overpotential of 18 mV at 10 mA cm −2 in alkaline seawater . More importantly, our Pt–Ni 3 S 2 /Co 9 S 8 –Sv electrode assembled into an alkaline electrolysis cell can work continuously for 50 h under alkaline seawater @ 60 °C. This work provides a promising strategy for designing highly surface-exposed single-atomic catalyst for large-scale hydrogen evolution through seawater electrolysis.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
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IF 9.4
Shan Xu,Dongxu Jiao,Xiaowen Ruan,Zhaoyong Jin,Yu Qiu,Jinchang Fan,Lei Zhang,Weitao Zheng,Xiaoqiang Cui
Efficient production of green hydrogen energy is crucial in addressing the energy crisis and environmental concerns. The oxygen evolution reaction (OER) poses a challenge in conventional overall water electrolysis due to its slow thermodynamically process. Urea oxidation reaction (UOR) offers an alternative anodic oxidation method that is highly efficient and cost-effective, with favorable thermodynamics and sustainability. Recently, there has been limited research on bifunctional catalysts that exhibit excellent activity for both OER and UOR reactions. In this study, we developed a selenium and iron co-doped nickel sulfide (SeFe-Ni 3 S 2 ) catalyst that demonstrated excellent Tafel slopes of 53.9 mV dec −1 and 16.4 mV dec −1 for OER and UOR, respectively. Density Functional Theory (DFT) calculations revealed that the introduction of metal (iron) and nonmetallic elements (selenium) was found to coordinate the d-band center, resulting in improved adsorption/desorption energies of the catalysts and reduced the overpotentials and limiting potentials for OER and UOR, respectively. This activity enhancement can be attributed to the altered electronic coordination structure after the introduction of selenium (Se) and iron (Fe), leading to an increase in the intrinsic activity of the catalyst. This work offers a new strategy for bifunctional catalysts for OER and UOR, presenting new possibilities for the future development of hydrogen production and novel energy conversion technologies. It contributes towards the urgent search for technologies that efficiently produce green hydrogen energy, providing potential solutions to mitigate the energy crisis and protect the environment.
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Environmental Science-Nano
·
2025
·
IF 5.1
Lu Wang,Siyuan Zhang,Jukun Xiong,Meicheng Wen
Formaldehyde (HCHO), a prevalent indoor volatile organic compound, poses significant health risks to humans even at very low concentrations. As a result, its efficient degradation at room temperature is of utmost importance. However, the detailed mechanism for catalytic oxidation of formaldehyde remains unclear, particularly regarding the role of water vapor, which has been subject to considerable debate. In this study, we synthesized Pt-supported TiO2 glass fiber catalysts (Pt/TiO2 GF) via NaBH4 reduction to investigate the reaction pathways and clarify the influence of water vapor on the oxidation process. The reaction mechanism and intermediates were systematically studied at room temperature using in situ diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS), kinetic isotope effect (KIE) analysis, and density functional theory (DFT) calculations. Results revealed a clear reaction pathway of HCHO → DOM → HCOO− → CO2, with formate C–H bond cleavage (HCOO−* + O* → CO2 + OH*) identified as the rate-determining step. Both experimental KIE results and theoretical calculations confirmed that water vapor substantially reduces the activation barrier for this step, significantly promoting formate decomposition and subsequent CO2 formation. This work provides critical insights into the role of water in enhancing formaldehyde oxidation and offers theoretical support for developing efficient catalysts suitable for humid environments.
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PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
·
2025
·
IF 7.8
Jinyu Shi,Xiunan Cai,Zhongqiu Luo,Chaochao Lai,Cuirong Yan,Qian Long,Shiyu Liu,Xintao Zhou
Green iron-based materials suffer from severe alkaline deactivation and limited photoactivity, restricting synergistic pollutant remediation. Herein, a bioinspired microenvironment engineering strategy to convert copper slag into amorphous SiO 2 -stabilized Mn-doped ferrous oxalate hollow tubus (MnFeOx@SiO 2 ). In the complex, amorphous SiO 2 neutralizes external alkalinity, while Si−OH dissociation maintains an weak-acidic microenvironment, preventing hydrolysis deactivation and ensuring robust photo-Fenton cycles. Experimental and DFT studies reveal that silicate-derived silica gel synergizes with glycerol to direct MnFeOx axial growth, forming channeled hollow tubes that enhance light harvesting and mass transfer. Concurrently, Mn doping builds Fe−O−Mn interfaces optimizing Fe d -band centers via d-d hybridization, accelerating charge separation. The catalyst showed exceptional performance under wide pH range (pH 2 −9), achieving 96.4 % methyl orange degradation and 98.2 % Cr(VI) reduction within 5 min. It exhibits 22.1/10.0-fold activity enhancement over FeC 2 O 4 under alkaline conditions and > 85.1 %/85.4 % stability after ten cycles. This work pioneers waste-derived catalysts for pH-universal water purification.
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