TRIBOLOGY INTERNATIONAL
·
2022
·
IF 5.62
Hongling Fang,Yi Li,Songwei Zhang,Qi Ding,Litian Hu
In this work, the functional protic ionic liquid (PIL) of dibutylammonium 3-(methylthio) propionate ([N44HH][MTP]) with good lubrication properties but poor oil solubility and the common PIL of dioctylammonium oleate ([N88HH][OA]) with good oil solubility but poor lubricating performance were selected. The binary ionic liquid (BIL) of [N88-N44][OA-MTP] was creatively obtained by combining [N44HH][[MTP] with [N88HH][OA]. Interestingly, the BIL has good oil solubility in PAO base oil and maintains outstanding lubricating performance, indicating that such an approach improves the oil solubility of functional PILs without worsening lubrication properties. In addition, the compatibility between two PILs and the lubrication mechanism of the BIL system were analyzed and described by means of several analytical methods.
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Frontiers in Neurology
·
2022
·
IF 4.086
Liao Hongsen,Li Haojia,Bao Hongguang,Jiang Li,Du Jiayue,Guo Yaoyi,Si Yanna
Objective: This study aims to analyze the changes of fecal short chain fatty acids (SCFAs) content and gut microbiota composition in sepsis associated encephalopathy (SAE) mice, further evaluating the effect of SCFAs on cognitive function and the underlying mechanism in SAE mice.Methods: A total of 55 male adult C57BL/6 mice (2–3 months of age, 20–25 g) were divided into four groups randomly: sham group (n = 10), cecal ligation and puncture group (CLP group, n = 15), CLP+SCFAs group (n = 15), and CLP+SCFAs+GLPG0974 group (n = 15). Seven days after surgery, fecal samples were collected for microbiota composition and SCFA analysis from 6 mice in each group randomly. Behavioral test was applied to assess cognitive impairment at the same time. After that, mice were sacrificed and brain tissue was harvested for inflammatory cytokines analysis.Results: The levels of acetic acid (.57 ± 0.09 vs 2.00 ± 0.24, p < 0.001) and propionic acid (.32 ± 0.06 vs .66 ± 0.12, p = 0.002) were significantly decreased in the CLP group compared with the sham group. The administration of SCFAs significantly increased the levels of acetic acid (1.51 ± 0.12 vs. 0.57 ± 0.09, p < 0.001) and propionic acid (0.54 ± 0.03 vs. 0.32 ± 0.06, p = 0.033) in CLP+SCFAs group compared with CLP group. Relative abundance of SCFAs-producing bacteria, including Allobaculum (0.16 ± 0.14 vs. 15.21 ± 8.12, p = 0.037), Bacteroides (1.82 ± 0.38 vs. 15.21 ± 5.95, p = 0.002) and Bifidobacterium (0.16 ± 0.06 vs. 2.24 ± 0.48, p = 0.002), significantly decreased in the CLP group compared with the sham group. The behavioral tests suggested that cognitive function was impaired in SAE mice, which could be alleviated by SCFAs pretreatment. ELISA tests indicated that the levels of IL-1β, IL-6, and TNF-α were elevated in SAE mice and SCFAs could lower them. However, the GPR43 antagonist, GLPG0974, could reverse the cognitive protective effect and anti-neuroinflammation effect of SCFAs.Conclusion: Our study suggested that in SAE, the levels of acetate and propionate decreased significantly, accompanied by gut microbiota dysbiosis, particularly a decrease in SCFAs-producing bacteria. GPR43 was essential for the anti-neuroinflammation and cognitive protective effect of SCFAs in SAE.
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CELLULAR AND MOLECULAR LIFE SCIENCES
·
2022
·Vol. 79
·期 8
·1-18
·
IF 9.207
Huang Ling,Zheng Junping,Sun Guangjun,Yang Huabing,Sun Xiongjie,Yao Xiaowei,Lin Aizhen,Liu Hongtao
Colitis develops via the convergence of environmental, microbial, immunological, and genetic factors. The medicine 5-aminosalicylic acid (5-ASA) is widely used in clinical practice for colitis (especially ulcerative colitis) treatment. However, the significance of gut microbiota in the protective effect of 5-ASA on colitis has not been explored. Using a dextran sulfate sodium (DSS)-induced colitis mouse model, we found that 5-ASA ameliorated colitis symptoms in DSS-treated mice, accompanied by increased body weight gain and colon length, and a decrease in disease activity index (DAI) score and spleen index. Also, 5-ASA alleviated DSS-induced damage to colonic tissues, as indicated by suppressed inflammation and decreased tight junction, mucin, and water–sodium transport protein levels. Moreover, the 16S rDNA gene sequencing results illustrated that 5-ASA reshaped the disordered gut microbiota community structure in DSS-treated mice by promoting the abundance of Bifidobacterium , Lachnoclostridium , and Anaerotruncus , and reducing the content of Alloprevotella and Desulfovibrio . Furthermore, 5-ASA improved the abnormal metabolism of bile acids (BAs) by regulating the Farnesoid X receptor (FXR) and Takeda G-protein-coupled receptor 5 (TGR5) signaling pathways in DSS-treated mice. In contrast, 5-ASA did not prevent the occurrence of colitis in mice with gut microbiota depletion, confirming the essential role of gut microbiota in colitis treatment by 5-ASA. In conclusion, 5-ASA can ameliorate DSS-induced colitis in mice by modulating gut microbiota and bile acid metabolism. These findings documented the new therapeutic mechanisms of 5-ASA in clinical colitis treatment.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Jinlan Nie,Xiaoting Fu,Lei Wang,Jiachao Xu,Xin Gao
This study evaluated the efficacy of Monascus purpureus fermentation on Saccharina japonica (SJ). Healthy substances and antioxidant activity of fermented SJ (FSJ) were determined. Results showed that fermentation caused the release of phenolic compounds and flavonoids, which resulted in the enhancement of antioxidant activity. Essential amino acids and γ-aminobutyric acid also greatly accumulated in FSJ. Sensory evaluation and gas chromatography-ion mobility spectrometry (GC-IMS) were used to evaluate flavor properties of FSJ. A lexicon consisted of 24 descriptors was established for SJ and FSJ, of which 14 descriptors were regarded as odor attributes. A total of 46 volatile compounds were identified by GC-IMS and showed positive correlation with odor attributes. Fifteen volatile compounds were screened as key compounds, tricarboxylic acid cycle, embden-meyerhof-parnas and amino acid catabolism were main formation metabolisms of them. Advanced properties of FSJ indicated that fermentation is a promising approach for the production of SJ food.
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Food & Function
·
2022
·Vol. 13
·期 17
·9119-9142
·
IF 6.317
Xiang Li,Yan Du,Chengping Zhang,Zhaoxin Tu,Li Wang
Highland barley (HB) displays a series of properties including regulation of lipid metabolism and attenuation of liver injury. Our study aimed to investigate the effect of modified highland barley (MHB) including fluidized highland barley (HB-1), extruded and puffed highland barley (HB-2), and ultrafine pulverized highland barley (HB-3) on lipid metabolism, liver inflammation, gut microbiota and metabolite profiles in mice fed with a high-fat/cholesterol diet (HFCD). 6 treatment groups were fed a normal control diet or an HFCD with or without MHB supplementation for 10 weeks. Results showed that MHB significantly improved lipid parameters, liver function and injury and blood glucose indexes related to hyperlipidemia compared with the HFCD group. In addition, MHB recovered the disorder of gut microbiota by increasing the Bacteroidetes/Firmicutes ratio and Lactobacillus and Allobaculum abundances and decreasing Proteobacteria abundance related to lipid metabolism bacteria. MHB reversed the decrease of short-chain fatty acid levels caused by the HFCD. Fecal metabolomics analysis showed that the important differential metabolites between HB-1 and HFCD were deoxycholic acid, myclobutanil and dibutyl phthalate, and the important differential metabolic pathways were arachidonic acid metabolism, ABC transporters and biosynthesis of unsaturated fatty acids. Results suggested that MHB especially HB-1 were better effective dietary intervention candidates to ameliorate hyperlipidemia compared with HB.
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CHROMATOGRAPHIA
·
2022
·Vol. 85
·期 9
·809-816
·
IF 2.213
Liu Yue,Lin Yuzi,Li Ping,Yang Huiwen,Huang Qingsong,Pan Yufang
Increasing numbers of studies suggest that short-chain fatty acids (SCFAs) are closely related to diabetes. However, the complexity of fecal pretreatment makes the detection of SCFAs in feces relatively difficult. In this study, we established a sample pretreatment protocol that involved no tedious steps, requiring only the grinding of appropriate amounts of feces into powder in liquid nitrogen. The SCFA content in feces was then detected using the headspace-gas chromatography (HS-GC) method. The method achieved good linearity ( R 2 ≥ 0.9940), and the limits of quantification (LOQs) and detection (LODs) of all investigated SCFAs ranged from 0.15 to 0.45 mM and 0.05 to 0.15 mM, respectively. The recoveries of the SCFAs ranged from 91.55 to 103.96% in mouse fecal samples. The HS-GC method was then applied to evaluate the effects of a neutral polysaccharide from Acanthopanax trifoliatus (ATP1-1) on the fecal SCFA content in streptozotocin (STZ)-induced diabetic mice. After 6 weeks of treatment with 140 mg/kg/d ATP1-1, the expression levels of acetic acid, propionic acid, butyric acid, isobutyric acid, isovaleric acid and valeric acid were increased by 215.4, 88.8, 95.3, 300.8, 260.7 and 158.0%, respectively, which indicated that the hypoglycemic effect of ATP1-1 may be related to its impact on SCFAs. Our simplified pretreatment of fecal samples is suitable for the analysis and detection of SCFAs in mouse feces by HS-GC.
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Food & Function
·
2022
·Vol. 13
·期 17
·8880-8891
·
IF 6.317
Juan Su,Xiong Fu,Qiang Huang,Guang Liu,Chao Li
The aim of this study was to elucidate the liberation and phytochemical profile of bound polyphenols present in dietary fiber (RPDF) isolated from Rosa roxburghii fruit pomace during in vitro-simulated digestion and colonic fermentation. The variation in the biological activity and prebiotic potential of the released polyphenols from RPDF was investigated. The results showed that the bound polyphenols were released from RPDF at a higher ratio during colonic fermentation than gastrointestinal digestion. The released polyphenols showed antioxidant activity and α-glucosidase inhibitory activity. The in vitro colonic fermentation assay showed that compared with dephenolized RPDF (DP-RPDF), RPDF exhibited a significantly stronger prebiotic effect by lowering the Firmicutes to Bacteroidetes (F/B) ratio at the phylum level, increasing the relative abundances of beneficial bacteria at the genus level, and improving the production of short chain fatty acids (SCFAs). These findings indicate that the bound polyphenols may act as important functional food ingredients that can contribute to the biological properties of RPDF. In addition, this study provides new insights into the high-value utilization of Rosa roxburghii fruit pomace, which will reduce the wastage of resources and greatly contribute to environmental protection.
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Sustainable Chemistry and Pharmacy
·
2022
·
IF 5.464
Zhenwei Zhang,Xiaoshi Ma,Chuanjun Yue,Xuejiao Wei,Baoliang Liu,Xiaohui Chen
Hazardous waste alkaline batteries and waste fatty acids significantly threaten the environment. In this study, waste alkaline batteries were simply transferred to r-MnZn catalysts, which were then applied in the ketonization of fatty acids. The assessment results show that r-MnZn can efficiently convert n-octanoic acid to 8-pentadecanone. The conversion and yield are evaluated as 100% and 92.9%, respectively, which are much better than those of TiO 2 , ZrO 2 , CeO 2 and other metal oxides . The excellent catalytic efficiency is derived from the MnO 2 nanorods in r-MnZn materials which are characterized by XRD, TEM, and XPS. In addition, when using different kinds of fatty acids in ketonization, longer carbon chains require a higher activation energy, and the conversion of fatty acids was lower. Furthermore, the ketone products have a short carbon chain that induces side reactions, leading to a low yield. The above results indicate that r-MnZn is an efficient catalyst applied in the ketonization of fatty acids.
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Fermentation-Basel
·
2022
·Vol. 8
·期 6
·262
·
IF 5.123
Jun Hao,Wen-Tao Sun,Chang-Rong Wu,Ming-Zhu Zhang,Guang-Hao Xia,Yu-Long Zheng,Chao Chen
Broussonetia papyriferaL. (paper mulberry) is an alternative woody plant, which can used to replace part of the protein feed for ruminants. Ensiling is an effective way to preserve fresh pasture and to solve the problem of stable storage and feed conversion of paper mulberry in the rapid growth period. However, low dry matter (DM), water-soluble carbohydrate, and lactic acid bacteria (LAB) reduce the quality of paper mulberry silage. This study assesses the influence of wilting time (0 h and 3.5 h; lighting: 3.43 × 104Lux) and three additives (Enterococcus durans, CL; cellulase, CE; and formic acid, FA) on the fermentation quality, aerobic stability, and bacterial community of whole plantB. papyriferasilage. The whole plantB. papyriferasample was mowed and wilted for 0 h and 3.5 h, and then had CL, CE, or FA added, followed by 60 days of ensiling. The results show all silage samples had high fermentation quality with pH below 4.2, ammonia-nitrogen below 100 g/kg DM, and no detectable butyric acid. The additives protected the DM and the crude protein from protease activity (p< 0.05), and CL was the most effective among them. Furthermore, wilting time influenced the silage’s bacterial communities, but overall, CL treatment had the greatest impact on bacterial communities. Wilting time and formic acid treatment significantly improved aerobic stability (p< 0.05).Enterococcuswas positively correlated with lactic acid (LA), while negatively correlated with LA andWeissella(p< 0.001). Enterococcuswas identified as the main driver of the whole plant paper mulberry ensiling process in the present study. In conclusion, compared to other additives, LAB is the most effective and economical to improve the fermentation quality and reduce the protein degradation of whole plant paper mulberry silage. Our findings provide a theoretical basis to improve the quality and production of paper mulberry silage.Keywords:paper mulberry;silage;wilting;additives;microbial community;aerobic stability
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Materials
·
2022
·Vol. 15
·期 10
·3645
·
IF 3.748
Minmin Zou,Ming Dong,Mingliang Luo,Hexin Zhu,Tian Zhao
Hierarchically porous MIL-101(Cr) (H-MIL-101(Cr)) with meso/macro-pores was directly prepared via nanofusion progress by using butyric acid as a modulating agent. In the methyl orange (MO) adsorption experiments, H-MIL-101(Cr) showed a high adsorption capability of 369.8 mg g−1, which was 1.52-fold greater than that of pristine MIL-101(Cr) (P-MIL-101(Cr)). While in the oxidation reaction of indene and 1-dodecene tests, H-MIL-101(Cr) presented much higher catalytic efficiency, with turnover frequency (TOF) values of 0.7242 mmol g−1min−1and 0.1492 mmol g−1min−1, respectively, which were 28% and 34% greater than that in the case of P-MIL-101(Cr). Thus, compared with P-MIL-101(Cr), H-MIL-101(Cr) exhibited better removal efficiency and higher levels of activity in the oxidation reactions of indene and 1-dodecene. The unique structure of H-MIL-101(Cr) also contributed to its superior performance in these processes.Keywords:hierarchically porous;MIL-101(Cr);butyric acid;MO adsorption;catalysis
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FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Qing Ge,Hang-qing Li,Zhe-yuan Zheng,Kai Yang,Peng Li,Zhu-qian Xiao,Guo-ming Xiao,Jian-wei Mao
Bamboo contains abundant hemicellulose, cellulose, and lignin, which are a high-quality insoluble dietary fiber (IDF) raw material. To investigate IDF- induced changes in the production of short-chain fatty acids (SCFAs) and the composition of human gut microbiota, IDF was extracted by alkaline hydrogen peroxide (named BIDF), complex enzymatic hydrolysis method (named OIDF) from bamboo, and commercial bamboo fiber BF90. The in vitro fecal fermentation characteristics of BIDF, OIDF, BF90 and its impacts on human gut microbiota were studied for the first time. Results showed that BIDF, OIDF, and BF90 could promote the production of total SCFAs after 24 h fermentation. Additionally, BIDF could alter the composition and microbial diversity of gut microbiota, especially increase the relative abundance of Bacteroides and decrease the ratio of Firmicutes to Bacteroidetes (F/B) value. These results revealed that the IDF from bamboo could be partially utilized by specific bacteria in human intestines and provide a reference for the study of the effects of IDF fermentation on SCFAs production and microbial composition.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Xu Jing,Haoyue Xue,Xiaoyan Sang,Xiaowen Wang,Liyan Jia
A simple, rapid, and efficient pretreatment method of mefentrifluconazole enantiomers in cereal samples was established by dispersive liquid–liquid microextraction coupled with ultra-high-performance liquid chromatography-diode array detector (UHPLC-DAD). In this study, a novel ternary magnetic deep eutectic solvent (MDES) [octyltrimethylammonium bromide][cobalt chloride][acetic acid] was synthesized as the extractant. Acetic acid was used as the dispersant to promote the in situ dispersion of binary MDES [octyltrimethylammonium bromide][cobalt chloride]. The microdroplets of binary MDES containing mefentrifluconazole were separated by an external magnet. Device-assisted dispersion and time-consuming centrifugation steps were eliminated to ensure simplicity and rapidity of the pretreatment. Good linearity ranging from 0.01 μg g −1 to 2 μg g −1 was obtained. The extraction recovery varied from 82.9 to 95.0%. The limit of detection was 0.003 μg g −1 . Finally, this established approach has been applied for the enantioselective detection of chiral mefentrifluconazole in corn, rice, wheat, millet, and sorghum samples.
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