Neurotherapeutics
·
2023
·
IF 5.7
Qiao Chen-Meng,Zhou Yu,Quan Wei,Ma Xiao-Yu,Zhao Li-Ping,Shi Yun,Hong Hui,Wu Jian,Niu Gu-Yu,Chen Yu-Nuo,Zhu Shan,Cui Chun,Zhao Wei-Jiang,Shen Yan-Qin
Accumulating data support a crucial role of gut microbiota in Parkinson’s disease (PD). However, gut microbiota vary with age and, thus, will affect PD in an age-dependent, but unknown manner. We examined the effects of fecal microbiota transplantation (FMT) pretreatment, using fecal microbiota from young (7 weeks) or aged mice (23 months), on MPTP-induced PD model. Motor function, pathological changes, striatal neurotransmitters, neuroinflammation, gut inflammation and gut permeability were examined. Gut microbiota composition and metabolites, namely short-chain fatty acids (SCFAs), were analyzed. Neurogenesis was also evaluated by measuring the number of doublecortin-positive (DCX + ) neurons and Ki67-positive (Ki67 + ) cells in the hippocampus. Expression of Cd133 mRNA, a cellular stemness marker, in the hippocampus was also examined. Mice who received FMT from young mice showed MPTP-induced motor dysfunction, and reduction of striatal dopamine (DA), dopaminergic neurons and striatal tyrosine hydroxylase (TH) levels. Interestingly and unexpectedly, mice that received FMT from aged mice showed recovery of motor function and rescue of dopaminergic neurons and striatal 5-hydroxytryptamine (5-HT), as well as decreased DA metabolism after MPTP challenge. Further, they showed improved metabolic profiling and a decreased amount of fecal SCFAs. High-throughput sequencing revealed that FMT remarkably reshaped the gut microbiota of recipient mice. For instance, levels of genus Akkermansia and Candidatus Saccharimonas were elevated in fecal samples of recipient mice receiving aged microbiota (AM + MPTP mice) than YM + MPTP mice. Intriguingly, both young microbiota and aged microbiota had no effect on neuroinflammation, gut inflammation or gut permeability. Notably, AM + MPTP mice showed a marked increase in DCX + neurons, as well as Ki67 + cells and Cd133 expression in the hippocampal dentate gyrus (DG) compared to YM + MPTP mice. These results suggest that FMT from aged mice augments neurogenesis, improves motor function and restores dopaminergic neurons and neurotransmitters in PD model mice, possibly through increasing neurogenesis.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Lu Wang,Zhen Li,Siying An,Huipeng Zhu,Xiaoyu Li,Dawei Gao
The aim of this study was to construct the nanoparticles based on Hohenbuehelia serotina polysaccharides and mucin for encapsulation of the polyphenols from Malus baccata (Linn.) Borkh (MBP-MC-HSP NPs), and investigate their effects on intestinal function and gut microbiota in mice. The results showed that MBP-MC-HSP NPs did not have any toxic and side effect by determining organ indexes and hematological parameters. The colonic index, colonic length as well as colonic histology were significantly improved by treatment of MBP-MC-HSP NPs. Moreover, MBP-MC-HSP NPs could increase the fecal moisture (84.71 %) and accelerate the intestinal peristalsis (77.87 %), thus reducing the defecation time (1.68 h) of mice at certain extent. Through production of acetic acid, propionic acid and n-butyric acid, MBP-MC-HSP NPs remarkably decreased the pH of colonic feces to maintain intestinal health. 16S rRNA sequencing analysis showed that MBP-MC-HSP NPs could improve the abundances of Lactobacillus , Butyicicoccus and Ruminococcus and suppress the richness of Prevotella , Bifidobacterium and Desulfovibrio , thereby optimizing the structure and composition of gut microbiota. Furthermore, the metabolic profiles of gut microbiota were influenced by MBP-MC-HSP NPs based on prediction of KEGG and COG databases. Overall, this study suggests that MBP-MC-HSP NPs can be developed and utilized as probiotics in the nutritional food field.
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FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Bing Xu,Shiqing Song,Lingyun Yao,Huatian Wang,Min Sun,Haining Zhuang,Xiaowei Zhang,Qian Liu,Chuang Yu,Tao Feng
Previous studies have shown that Ficus carica polysaccharide (FCPS) extracted from common fig fruit exhibits antioxidant and immune-enhancing properties. Nevertheless, it remains uncertain whether FCPS can modulate the metabolism of gut microbiota. This study aims to assess whether the polysaccharides from two different types of figs can be broken down by the upper digestive system, including saliva, simulated stomach, and small intestinal conditions, and whether the gut microbiota can utilize them (Mw = 4.59 × 10 4 (LFCP, Low molecular weight), Mw = 6.08 × 10 4 (HFCP, High molecular weight)). The results revealed that FCPS retained its molecular weight and reducing sugar content even after passing through the upper digestive system, indicating its safe arrival in the gut without undergoing decomposition. In vitro FCPS fermentation of gut microbiota lowered the pH and produced more gas in the order of LFCPHFCP, respectively, indicating that it can be broken down and utilized by the microbiota. Additionally, the amount of short-chain fatty acids increased notably (LFCP < HFCP), indicating the higher the molecular weight, the stronger the acid production capacity. FCPS demonstrated the ability to significantly decrease the Firmicutes to Bacteroidetes ratio, potentially inhibiting obesity and inflammation. Therefore, FCPS is a promising functional food with potential to enhance health and prevent disease by promoting gut health.
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Food & Function
·
2022
·Vol. 13
·期 20
·10759-10768
·
IF 6.317
Meng Jia,Dengdeng Li,Rui Wang,Anqi Wang,Padraig Strappe,Qinghai Wu,Wenting Shang,Xuanyu Wang,Min Zhuang,Chris Blanchard,Zhongkai Zhou
The influence of phenolic compound extracts from three colored rice cultivars on the gut microbiota was investigated. The results revealed that protocatechuic acid, chlorogenic acid, caffeic acid and p-coumaric acid were the major metabolites after gut microbiota fermentation. The presence of phenolic compounds led to a significantly decreased ratio of Firmicutes and Bacteroidetes, while the abundance of Proteobacteria decreased. At the genus level, phenolic compounds promoted an increase of Prevotella, Megamonas and Bifidobacterium, while the abundance of Bacteroides and Escherichia–Shigella was inhibited. The concentration of ferulic acid and syringic acid was positively correlated with Bifidobacterium, while Megamonas was positively correlated with catechin and caffeic acid. The abundance of Escherichia–Shigella and Citrobacter was found to be significantly negatively correlated with chlorogenic acid. More importantly, this study revealed that the presence of phenolic compounds generated more propionate, followed by acetate, but not butyrate after gut microbiota fermentation.
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GREEN CHEMISTRY
·
2022
·Vol. 24
·期 19
·7644-7651
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IF 11.034
Linfeng Zhang,Changpo Ma,Chenyu Wang,Guangyu Sun,Yongqi Shu,Wenya Zou,Tao Yang,Weiran Yang
Glyceric acid (GA), a glycerol derivative, can be used as a versatile platform molecule to produce value-added chemicals. Succinic acid (SA) is a very important bio-based molecule that acts as a fine chemical and is used in biodegradable polymer synthesis. It has been shown earlier that GA can be effectively converted to the platform chemical 3-iodopropionic acid (3-IPA) with a RhCl3/HI catalyst system. In this work, we first explored the production of SA from 3-IPA by carbonylation. With RhCl3 as the catalyst and acetic acid (AA) and water as the solvent, as high as 78% yield of SA can be achieved. Considering the similarity of the catalysts used in two steps, we then further probed the one-step synthesis of SA directly from GA. Under optimized reaction conditions, 71% yield of SA can be obtained directly from GA by reductive carbonylation with RhCl3 and I2 as the catalyst under H2 and CO atmospheres. This is the first report of the direct conversion of GA to SA, which provides a novel method for glycerol valorization.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
·
IF 4.831
Jinming Xu,Hao Yuan,Huangmei Zhou,Yu Zhao,Ying Wu,Jie Zhang,Sanjun Zhang
Baijiu occupies a vital position in Chinese food and China's market. Strict evaluation of Baijiu is highly demanded. In this study, we constructed a novel fluorescent sensor array based on the single glutathione-protected gold nanoclusters (AuNCs) probe for the detection of organic acids and Baijiu. The fluorescence of AuNCs was simply modulated by three metal ions (Cu 2+ , Mn 2+ , and Ag + ), and formed new complexes as sensing elements. These four sensing elements responded variously to nine organic acids, and further chemometric analysis results allowed for the classification and quantification of acids. Moreover, the sensor array successfully identified 21 Baijiu samples of different brands among 11 aroma types. It could also distinguish Baijiu of different qualities as well as pure Baijiu from its adulterations and showed high selectivity among multiple interfering drinks. The results demonstrated that the AuNCs-based sensor array has considerable potential for quality monitoring of Baijiu.
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DALTON TRANSACTIONS
·
2022
·Vol. 51
·期 38
·14639-14645
·
IF 4.569
Wenhui Zhang,Yiming Wang,Kelong Ding,Hong Li,Zejun Sun,Ze-Nan Hu,Hong-bin Sun
Semi-hydrogenation usually requires an effective catalyst to ensure selectivity, especially when reducible groups coexist in a molecule. Pd is widely used in the semi-hydrogenation of alkynes to synthesize alkenes, but the selectivity control is still challenging. Herein, we design a catalyst with a semi-encapsulated PdRh alloy heterojunction in a carbon layer for the selective semi-hydrogenation of 3-nitrophenylacetylene (3-NPA). Benefiting from the presence of a PdRh alloy heterojunction and a semi-encapsulated structure, the catalyst delivers good selectivity and maintains high activity. In addition, the carbon shell can ensure the stability of the catalyst and prolong the service life. This study provides ideas for the rational design of a catalyst to achieve a selective hydrogenation reaction for practical applications.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Chuang Liu,Zhichang Qiu,Duanyin Gu,Fangzhou Wang,Rentang Zhang
This study explored the structural characteristics of the purified blackened polysaccharide fraction (BJP-4) and its potential to alleviate colitis. BJP-4 had a molecular weight of 1.24 × 10 5 Da and mainly consisted of → 4)- α - l -Gal p A (1→, →5)- α - l -Ara f (1 → and → 4)- β - d -Gal p (1 → residues. After the administration of BJP-4, the disease activity index of colitic mice was reduced by 40.38–50.96%, accompanied by increased colon length (30.00–62.50%) and ameliorated pathological damage. The mechanisms involved regulation of the intestinal microbiota and subsequent inhibition of the TLR4/MyD88/NF- κ B/NLRP3 pathway, balance of pro/anti-inflammatory cytokines and oxidative status, enhancement of epithelial barrier integrity and production of short-chain fatty acids. In particular, high-doses of BJP-4 significantly increased the abundance of beneficial bacteria (f_ Muribaculaceae , Lactobacillus and Lachnospiraceae _NK4A136_group, 0.78–1.04-fold) and suppressed the levels of harmful bacteria (o_ Clostridia _UCG-014, Bacteroides and Escherichia-Shigella , 32.30–90.42%). These findings revealed the anti-inflammatory effects and mechanisms of purified blackened polysaccharides, which facilitated their application in functional foods.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 103
·期 8
·3840-3849
·
IF 4.125
Ying You,Haoran Song,Linlin Wang,Zhengqi Liu,Xiaoming Guo,Chunqing Ai,Shuang Song,Beiwei Zhu
BACKGROUND Caulerpa lentillifera has received extensive attention regarding expansion of its farming and increasing consumption. In our previous study, the structure of C. lentillifera polysaccharide (CLP) was elucidated. However, little information is available about its health effects. In this study, the anti-obesity effect of CLP was investigated by using a high-fat diet-induced obese mice model with two different supplementation methods. RESULTS In vitro simulated digestion results showed that CLP significantly decreased the lipid digestibility and induced the lipid droplets aggregation in the intestinal stage to inhibit the absorption of lipids. As revealed by 16S ribosomal RNA sequencing and non-targeted metabolomics, supplement of CLP by both pre-prandial gavage and free feeding patterns effectively prevented mice obesity via ameliorating intestinal flora disturbance and regulating bile acids circulation metabolism. Of note was that CLP administration had no effect on short-chain fatty acids production, suggesting the anti-obesity effect was uncorrelated with their production. Moreover, pre-prandial administration of CLP had a better anti-obesity effect in lowering body weight and serum lipid levels, but the free feeding resulted in a higher α -diversity of gut microbiota. CONCLUSION The findings of this study indicate that CLP could be a potential anti-obesity nutraceutical and that pre-prandial supplement of CLP may be a better intake method to exhibit its hypolipidemic effect. © 2022 Society of Chemical Industry.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 10
·1-12
·
IF 6.408
Liao Linhong,Tong Simiao,Luo Xiaogang,Liu Genyan,Wu Fengshou
A new iron porphyrin-based organic polymer (Fe-POP) was synthesized through the William ether reaction. The as-prepared Fe-POP presented high chemical stability, wide pore distribution, high iron content, and strong affinity with 3,3’,5,5’-tetramethylbenzidine (TMB) and hydrogen peroxide (H 2 O 2 ), which contributed to its excellent peroxidase-mimicking performance. In the presence of H 2 O 2 , Fe-POP could catalyze the transparent TMB into blue ox-TMB, which could be easily distinguished by the naked eyes. Moreover, glutathione (GSH) and ascorbic acid (AA) could convert blue ox-TMB into colorless TMB due to the inhibitory effect of GSH/AA to the catalytic oxidation of TMB. Based on this phenomenon, a rapid and sensitive colorimetric method for the assay of H 2 O 2 , GSH, and AA was developed using Fe-POP as sensor. The detection limits of H 2 O 2 , GSH, and AA were 1.37, 0.44, and 0.33 μM, respectively. Finally, the colorimetric method based on Fe-POP was used to evaluate the GSH and AA content in real samples, which provided the guidance for GSH and AA supplements in our daily diet, suggesting the significant potential of Fe-POP in practical applications. Graphical abstract
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Journal of Inflammation Research
·
2022
·
IF 4.631
Jiao Peng,Xi Li,Lin Zheng,Lifang Duan,Zhengxian Gao,Die Hu,Jie Li,Xiaofeng Li,Xiangchun Shen,Haitao Xiao
Purpose GLP-1 based therapy represents a new treatment option for inflammatory bowel disease. Ban-Lan-Gen (BLG) granule, a known anti-viral TCM formulation, exhibits potential anti-inflammatory activities in treating various kinds of inflammation. However, its anti-inflammatory effect on colitis and the underlying mechanisms remain unknown.Methods Dextran sulfate sodium (DSS)-induced chronic relapsing colitis in mice was established. The disease activity index, histological sign of damage, and levels of proinflammatory cytokines were performed to assess the protective effects of BLG. Serum GLP-1 level and colonic Gcg, GPR41 and GRP43 expression, the community compositions of gut microbiota, the levels of SCFAs in the feces and GLP-1 release from primary murine colon epithelial cells were performed to characterize the effects of BLG on gut microbiota and gut SCFA derived-GLP-1 production.Results BLG treatment significantly alleviated body weight loss, DAI, colon shortening, colon tissue damage, and pro-inflammatory cytokine levels of TNF-α, IL-1β and IL-6 in the colon tissues. Moreover, BLG treatment could observably restore colonic Gcg, GPR41 and GRP43 expression and serum GLP-1 level of colitic mice, as well as correct the alteration of gut microbiota in colitic mice by increasing the abundances of SCFA-producing bacteria, eg, Akkermansia and Prevotellaceae_UCG-001, and decreasing the abundances of bacteria, eg, Eubacterium_xylanophilum_group, Ruminococcaceae_UCG-014, Intestinimonas, and Oscillibacter. Furthermore, BLG treatment could markedly increase the levels of SCFAs in feces of colitic mice. In parallel, ex vivo assay also showed that and the extract of feces from BLG-treatment mice could greatly stimulate the secretion of GLP-1 from primary murine colon epithelial cells.Conclusion These findings suggest that the anti-colitis effects of BLG are achieved at least partly by regulating gut microbiota and restoring gut SCFA derived-GLP-1 production, and BLG has the potential to be developed as a promising agent for the treatment of chronic relapsing colitis.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Meng-Xin Hu,Fei He,Ya-Xin Guo,Li-Zhen Mo,Xuan Zhu
Bacteria colonizing the gastrointestinal tract generally grow well in biofilms. In recent years, probiotic biofilms have been considered the most promising fourth-generation probiotics. However, the research into the functions of probiotic biofilms is just starting. In this study, Lactobacillus reuteri DSM 17938 biofilms formed on electrospun cellulose acetate nanofibrous scaffolds were contrasted with planktonic cells. Pathogen inhibition analysis of Escherichia coli, Staphylococcus aureus, and Listeria monocytogenes suggested a significant distinction between the planktonic and biofilm groups. In human fecal fermentation, L. reuteri remodeled the microbiota by decreasing the relative abundances of Proteobacteria, Escherichia–Shigella, and Desulfovibrio and increasing the relative abundances of Phascolarctobacterium, Bacteroides, and Lactobacillus. Moreover, L. reuteri biofilms played more positive roles in microbiota modulation and short-chain fatty acid production than planktonic L. reuteri. These findings provide an understanding of the beneficial effects of probiotic biofilms, laying a foundation for the application of probiotic biofilms as a health promoter.
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