JOURNAL OF CHROMATOGRAPHY A
·
2016
·
IF 3.926
Sheng Tang,Jian-De Liu,Qin Bin,Ke-Qin Fu,Xiao-Chen Wang,Ying-Bin Luo,Shao-Hua Huang,Zheng-Wu Bai
In order to comprehensively understand the influence of coordination of the substituent at 2-position with those at 3- and 6-positions on the properties of chitosan derivatives, a series of chitosan 3,6-bis(arylcarbamate)-2-(amide)s (CACAs) and the related chiral stationary phases (CSPs) were prepared and reported in the present study. Specifically, chitosan was N -acylated with carboxylic acid anhydrides, and then further derivatized with various aryl isocyanates to afford CACAs, from which a class of coated-type CSPs were prepared. When the substituent introduced on the acyl group at 2-position and those on the phenyl group of the carbamates at 3- and 6-positions were fittingly combined, these prepared CACAs based CSPs would exhibit powerful chiral recognition ability, further resulting in a class of promising chiral separation materials with excellent enantioseparation performance. Meanwhile, these newly developed materials with suitable molecular weight also bear a high tolerability towards organic solvents, even including pure tetrahydrofuran, thus broadening their application in enantiomeric separation.
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JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Yanhui Zhang,Hongbo Li,Xiaoqin Hai,Xiaoli Guo,Xin Di
A green and recyclable switchable supramolecular deep eutectic solvent (SS-DES) was designed and prepared for effective extraction of flavonoids from Scutellariae Radix . The novel SS-DES has both excellent extraction performance of DES and the host guest inclusion of cyclodextrin, thereby showing superior extraction efficiency and selectivity. The characteristic of polarity switching can endow the SS-DES with achieving homogeneous extraction and rapid two-phase separation, shorting per-treatment time largely. Parameters affecting the extraction performance were investigated by the response surface methodology. The results indicated that the SS-DES showed better extraction yield of total flavonoids (157.95 mg/g) compared with pure DES (135 mg/g) and traditional organic solvent (60 % ethanol, 104.87 mg/g). Moreover, the switching mechanism of SS-DES was characterized by FT-IR and 1 H NMR, and the extraction mechanism was studied by density functional theory and molecular docking analysis. After evaluating the ecological impact of the method, the cytotoxicity of SS-DES was investigated and the result displayed that its toxicity was very low or even negligible with the EC 50 >2000 mg/L. After being adsorbed by macroporous AB-8 resin, the regenerated SS-DES was recycled 5 times and the extraction efficiency still remained above 90 %, indicating the desirable reusability. Therefore, the proposed method was efficient and sustainable, and revealed favorable application prospect for the extraction of bio-active compounds from plant materials.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Xiaojiao Zheng,Xingle Guo,Yawen Zhang,Jiajun Han,Xu Jing,Junxue Wu
A novel, environmentally friendly, and efficient method for determining triazine herbicides in water, tea, and juice was developed by combining magnetic dispersive micro-solid phase extraction (MD-μSPE) with magnetic dispersive liquid-liquid microextraction (MDLLME), followed by high-performance liquid chromatography (HPLC). The pretreatment process, utilized magnetic biochar (MBC) and magnetic deep eutectic solvent (MDES) as the adsorbent and extractant, respectively. Fe(NO 3 ) 3 was loaded onto waste mushroom sticks to prepare MBC via impregnation-pyrolysis, while tri- n -butylphosphine oxide, nonanoic acid, and FeCl 3 were combined through hydrogen bonds to form MDES. This approach significantly reduced environmental impact using waste materials and shortened separation times. The method demonstrated excellent linearity (1–100 μg L −1 ), low detection limits (0.3 μg L −1 ), and good precision (RSDs <6.5 %). Recoveries in water, tea, and juice ranged from 80.1 % to 90.6 %. This combined MD-μSPE-MDLLME approach offers a greener and more efficient extraction process for separating and concentrating pesticides from complex food samples.
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FISH & SHELLFISH IMMUNOLOGY
·
2024
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IF 4.1
Nanhai Xiao,Xiuzhen Zhou,Yi Zhang,Tao Liu,Yiwei Jiang,Sami Ullah,Jing Wang,Fengqin Feng,Minjie Zhao
The aim of this study was to investigate the effects of antibacterial peptides from Brevibacillus texasporus (BT) on the growth performance, meat quality and gut health of cultured largemouth bass ( Micropterus salmoides ). Largemouth bass (36.17 ± 1.52 g) were divided into 2 groups and each group was fed with diets supplemented with or without 200 ppm of BT peptides for 130 days. The results showed that BT peptides had no significant influences on growth performance and body indexes, but significantly enhanced total antioxidant capacity and lysozyme content in the serum. Moreover, digestive enzymes activities and intestinal villous height were also prominently increased. From meat quality aspect, no significant differences were found in nutritional components, amino acid composition, fatty acid composition and texture property, except the values of hardness, gumminess and γ-linolenic acid (C18:3n6) were remarkably increased after BT peptides intervention. Finally, the results of gut microbiota and short chain fatty acids revealed that BT peptides significantly decreased the relative abundances of harmful bacteria such as genus Acinetobacter and Pseudomonas , and increased the production of short chain fatty acids. In conclusion, this study confirmed that BT peptides could be used to improve the health of largemouth bass, which provided novel insights into the application of antimicrobial peptides in aquacultures.
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JOURNAL OF AGRICULTURAL SCIENCE
·
2024
·Vol. 161
·期 6
·877-886
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IF 1.7
Xinbao Li,Haopeng Liu,Tongtong Dai,Dong Dong,Guofeng Xu,Yushan Jia,Tao Shao
The work aimed to evaluate the effect of lactic acid bacteria, natamycin and hexanoic acid on the fermentation quality, aerobic stability and in vitro digestibility of total mixed ration (TMR) silages containing water bamboo sheath leaves. The treatments were as follows: (1) no additives (control, C), (2) lactic acid bacteria (L), (3) natamycin (N), (4) hexanoic acid (H), (5) lactic acid bacteria + natamycin (LN), (6) lactic acid bacteria + hexanoic acid (LH). Silos were opened and silages were evaluated to assess fermentation quality, aerobic stability and in vitro digestibility. All TMR silages were well-preserved as indicated by dominant lactic acid content, low ammonia nitrogen/total nitrogen, trace propionic acid and negligible butyric acid contents. All additives improved the fermentation quality and in vitro digestibility, evidenced by higher lactic acid/acetic acid ratio, lactic acid, acetic acid, water-soluble carbohydrate contents and lactic acid bacteria count, and lower pH, aerobic bacteria and yeast counts. The LN had the highest lactic acid, acetic acid, lactic acid/acetic acid ratio, in vitro gas production and in vitro digestibility. During aerobic exposure, the LN silage had the highest lactic acid and acetic acid contents, and the lowest pH among all TMR silages. The LN and LH prolonged aerobic stability, reaching 85.5 and 81.5 h, respectively. The LN is recommended to improve the fermentation quality, aerobic stability and in vitro digestibility of TMR prepared with water bamboo sheath leaves.
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CRYSTAL GROWTH & DESIGN
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2024
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IF 3.2
Ronghua Peng,Xueyou Qin,Jiawei Lin,Lingyu Wang,Songgu Wu,Junbo Gong
We designed a solvent mixture composed of carboxylic acid solvents and water as the crystallization medium for the selective preparation of three distinct γ-aminobutyric acid (GABA) conformational polymorphs (Form-I, Form-II, and the newly discovered Form-IV). By adjusting the carbon chain length and content of carboxylic acid solvents, we were able to finely tune the solvation environment, leading to the formation of the desired polymorphs. Crash-cooling crystallization experiments demonstrated that with the carbon chain length (C1–C6) and content (0–30%) of carboxylic acid increasing, the polymorphic outcomes gradually change from Form-I to Form-II and ultimately to Form-IV. Interestingly, four different conformations were observed within these three polymorphs. DFT calculation indicated that the stability order of these conformations is conformer-IVβ > conformer-IVα > conformer-II > conformer-I. Based on solution FTIR analysis and solvation free energy calculation, the solvation effect on GABA molecules diminishes with an increasing carbon chain length and content of carboxylic acid solvents. A lower solvation effect makes the desolvation process of GABA molecules much easier so that GABA molecules can undergo sufficient conformational rearrangement during the nucleation stage, thereby adopting a more stable conformation during the molecular assembly process. In this crystallization conditions, Form-IV with a more stable conformation in its crystal structure has the potential to replace Form-II and Form-I through preferential crystallization.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
·
IF 4
Min Wang,Yajie Yue,Jingjing Zhang,Yifan Qin,Liyan Jia,Xu Jing
In this study, magnetic deep eutectic solvent-dispersive liquid-liquid microextraction combined with high-performance liquid chromatography method was developed for the extraction and determination of succinate dehydrogenase inhibitors in water, juice, wine, and vinegar . A new green magnetic deep eutectic solvent was synthesized using quaternary phosphorus salt, metal chloride, and fatty acid. The special self-dispersion magnetic deep eutectic solvent achieved rapid extraction without auxiliary dispersion and separation via neodymium magnets without centrifugation, avoiding the use of toxic disperser solvents and disperser instruments and reducing the separation time. The recovery of succinate dehydrogenase inhibitors under optimal extraction conditions was 76.5–97.8 %, and the limit of detection was 0.0011–0.0029 mg L −1 . The method could be a fast, simple, green, and reliable alternative for the analysis of succinate dehydrogenase inhibitors. The self-dispersion magnetic deep eutectic solvent can facilitate the development of dispersive liquid-liquid microextraction methods of other pesticides in food samples.
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Heliyon
·
2024
·
IF 3.4
Yimeng Ren,Longzhu Zhou,Yujie Shi,Yanan Yu,Weihai Xing,Qingyu Zhao,Junmin Zhang,Yueyu Bai,Jing Li,Chaohua Tang
Beef flavor profiles are strongly influenced by cooking methods and lipid composition. However, the effect of instant-boiling on the aroma of different beef slices was unclear. This study investigated the lipid profiles and instant-boiling volatile profiles of chuck tender (M. Supraspinatus ), sirloin (M. Longissimus dorsi ) and silverside (M. Biceps femoris ). Quantitative lipidomics identified 336 lipid molecular species, of which 84–112 were quantitatively different among the three beef slices. Sirloin had lower phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol and free fatty acids than chuck tender and silverside. The unsaturated fatty acid acyl chains in phosphatidylethanolamine differed significantly. Solid phase microextraction-gas chromatography-olfactometry-mass spectrometry (SPME-GC-O-MS) identified hexanal, octanal, nonanal, decanal, (E)-2-octenal, (E)-2-nonenal, (E)-2-undecenal, (E,E)-2,4-nonadienal, (E,E)-2,4-decadienal, 1-octen-3-ol, 2-pentylfuran and acetoin as the aroma-active compounds of instant-boiled beef. Unsaturated free fatty acids and phosphatidylglycerols with unsaturated fatty acid residues positively correlated with the aroma-active compounds and might be crucial in flavor differences among the three beef slices. These findings provide greater understanding of the lipid and instant-boiling aroma-active compound profiles in chuck tender, sirloin and silverside, and reflect the suitability of different beef slices for instant-boiling from the aroma perspective.
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ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Jiaze Yan,Chao Jiang,Jiqi Zheng,Ming Guo
Background Microdialysis is an in vivo technique for dynamically analyzing biochemical components in tissues and fluids. Traditional microdialysis systems are primarily designed for low molecular weight polar compounds, resulting in lower recoveries for hydrophobic compounds. This study aimed to develop a greener, cost-effective, and enhanced microdialysis method for recovering eugenol, isoeugenol, and methyl isoeugenol, utilizing hydrophobic deep eutectic solvents as the perfusate, with the method's feasibility verified through in vivo experiments. Results Among the 19 types of deep eutectic solvents evaluated, thymol-levulinic acid was identified as the most suitable for eugenol compounds based on liquid-liquid microextraction and microdialysis experiments, showing an in vitro microdialysis recovery approximately 100 times higher than that of water. The optimization of microdialysis parameters was conducted, including molar ratio of HBA:HBD, temperature, flow rate, and semi-permeable membrane length. In the in vivo microdialysis experiments, the temporal concentration of eugenol, which was used as an anesthetic in Glyptocidaris crenularis, was detected by GC-MS/MS and calibrated using ex vivo recovery. When the concentration of eugenol in artificial seawater was maintained at 100 μg mL −1 , the eugenol levels in the coelomic fluid of Glyptocidaris crenularis rose from 0 to 6.89 ± 0.38 μg mL −1 within 60 min, then stabilized in next 80 min. Significance The findings demonstrate that deep eutectic solvents enhanced microdialysis provides high recoveries and enables real-time monitoring of drug concentration changes in biological systems, making this method a valuable tool for pharmacokinetic studies and environmental monitoring.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Nan Ma,Rong Li,Gui-Fang Zhang,Ruo-Han Gao,Dong-Jie Zhang
Quinoa β-glucan (QBG) has shown potential benefits in treating type 2 diabetes mellitus (T2DM); however, comprehensive evaluations of its effects remain limited. This study investigates the impact of QBG—derived from hot water extraction (Q-) and microbial fermentation enrichment (Q+)—on serum glucose levels, lipid profiles, appetite-regulating hormones, fecal short-chain fatty acids (SCFAs), and gut microbiota composition and function in streptozotocin/high-fat diet (STZ/HFD)-induced T2DM mice. The results indicate that QBG treatment significantly reduced fasting blood glucose, insulin levels, triglycerides (TG) and total cholesterol (TC), while concurrently increasing high-density lipoprotein cholesterol (HDL C) levels. Additionally, liver and pancreatic function improved, as evidenced by decreased levels of malondialdehyde (MDA), aspartate transaminase (AST), and alanine transaminase (ALT). SCFA levels were significantly higher in QBG-treated groups compared to MC group. QBG treatment also reduced the abundance of Firmicutes and Patescibacteria, along with the Firmicutes/Bacteroidota ratio, while increasing levels of Bacteroidota and Actinobacteria. These findings suggest that QBG can regulate the dysbiosis of SCFAs production in T2DM mice and may indirectly modulate the secretion of appetite-regulating hormones by influencing gut microbiota composition. Furthermore, PICRUSt analysis revealed that QBG treatment, particularly Q + _H, could enhance disrupted metabolism and improve gut microbiota functions, helping restore normal physiological function.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Wenqiang Liu,Zitong Qi,Tuanning Liu,Yang Zhang
Inorganic halide perovskite nanocrystals (NCs) are regarded as promising emitters for light-emitting diodes due to their bright and narrow emission. However, surface defects often result in trap states and ion migration, which remains a huge challenge for high-quality perovskite NCs. Herein, fluoride ions are introduced into CsPbBr3 perovskite NCs at room temperature through the chelation of ligands. Experimental results demonstrate that these fluoride ions from inorganic salts can improve the average lifetime and crystallinity of CsPbBr3 NCs. Meanwhile, the resulting photoluminescence quantum yield is optimized up to 99.02%, and it has high stability to water, heat, and ultraviolet light. Density functional theory calculations show that fluoride ions have a higher binding energy compared to other ligands, which not only removes the electron trapping center but also increases the halogen ion migration energy. By mixing green-emission CsPbBr3 NCs and red-emission K2SiF6:Mn4+ phosphors on a blue chip, the fabricated white light emitting diode shows a high luminous efficiency of 147.8 lm/W, a wide color gamut (129% for NTSC), and CIE coordinates of (0.3160, 0.3051). Furthermore, the photoluminescence intensity decreased by only 2.9% after 48 h of continuous operation.
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Foods
·
2024
·Vol. 13
·期 10
·1561
·
IF 4.7
Qiaoyi Zhou,Jinjing Gao,Xueyan Sun,Yicheng Liang,Minqi Ye,Dongxia Liang,Caijin Ling,Binghu Fang
Tea plants have a long cultivation history in the world, but there are few studies on polysaccharides from fresh tea leaves. In this study, tea polysaccharides (TPSs) were isolated from fresh tea leaves. Then, we investigated the characteristics of TPSs during in vitro simulated digestion and fermentation; moreover, the effects of TPSs on gut microbiota were explored. The results revealed that saliva did not significantly affect TPSs’ molecular weight, monosaccharide composition, and reducing sugar content, indicating that TPSs cannot be digested in the oral cavity. However, TPSs were partially decomposed in the gastrointestinal tract after gastric and intestinal digestion, resulting in the release of a small amount of free glucose monosaccharides. Our in vitro fermentation experiments demonstrated that TPSs are degraded by gut microbiota, leading to short-chain fatty acid (SCFA) production and pH reduction. Moreover, TPSs increased the abundance of Bacteroides, Lactobacillus, and Bifidobacterium but reduced that of Escherichia, Shigella, and Enterococcus, demonstrating that TPSs can regulate the gut microbiome. In conclusion, TPSs are partially decomposed by gut microbiota, resulting in the production of SCFAs and the regulation of gut microbiota composition and function. Therefore, TPSs may be used to develop a prebiotic supplement to regulate the gut microbiome and improve host health.
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