FOOD RESEARCH INTERNATIONAL
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2020
·
IF 4.972
Jinyan Gu,Haihui Zhang,Chaoting Wen,Jixian Zhang,Yuanqing He,Haile Ma,Yuqing Duan
As a healthy food and traditional Chinese medicine, sagittaria sagittifolia L. has been used for a long history. Nevertheless, reports on the bioactivity and chemical characterization of S. sagittifolia L. polysaccharides are still very rare. In this paper, ultrasound-assisted method (UAE) was used to extract S. sagittifolia L. polysaccharides, after alcohol precipitation and column chromatography isolation, the structural characteristics, antioxidant and immunological activities of the purified polysaccharide (SPU60-W) were preliminarily investigated. The results indicated SPU60-W (16.62 kDa) was a pyranoid polysaccharide containing α-glycosidic bond composed of mannose, xylose, and glucose with a molar ratio of 2.69: 2.04: 95.27. It consisted of slender wormlike strands, which may involve some degree of aggregation of helices, as well as a small proportion of irregular spherical structures. Furthermore, antioxidant activity analysis showed that SPU60-W possess excellent hydroxyl and ABTS radical scavenging activity comparable to vitamin C (Vc), and moderate DPPH radical scavenging activity. Immunity tests suggested that SPU60-W significantly promoted the proliferation, phagocytosis and NO production of mouse macrophage RAW264.7. According to this study, SPU60-W might be utilized as a potent antioxidant and immunomodulator in food and medicinal industry.
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TALANTA
·
2020
·
IF 5.339
Yuting Li,Xing Guo,Yonghui Tian,Tianlong Zhang,Zewei Luo,Xiaojiao Liu,Cheng Qian,Jianxiong Dai,Yixiang Duan
Obtaining the fingerprints of polysaccharides was known to be notoriously difficult by ambient mass spectrometry due to their resistance for desorption and ionization. Ambient mass spectrometry technology has recently been recognized as a quick analysis tool for obtaining fingerprints, which is attributed to its characteristics of no sample pretreatment and easy operation under atmospheric pressure. However, it still remains a challenge for accurate identification of the fingerprints of macromolecular polysaccharides by ambient micro-fabrication glow discharge plasma (MFGDP). In this study, a simplified methylation method was introduced to realize rapid analysis of polysaccharide mixture by MFGDP with the assistance of a temperature-programmed system (TPS). At the optimal temperature of TPS, oligosaccharides , plant polysaccharides and polysaccharide mixtures were all well characterized by TPS-MFGDP. In the proposed method, the characteristic [M + NH 4 ] + adduct ions of oligosaccharides ions of oligosaccharides that are difficult for other ambient mass spectrometric methods were abundantly produced, making it possible to simultaneously identify a mixture of five or more polysaccharides. In addition, a supervised classification model which based on MS spectra, was used to classify seven typical hypoglycemic polysaccharides with excellent sensitivity, specificity and accuracy, indicating the good classification performance of the RF model constructed. Thus, the proposed mass spectroscopic method provides a cost effective, accurate and high throughput tool for identification and classification of polysaccharides, which is beneficial for studying the biological activity of polysaccharides.
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INDUSTRIAL CROPS AND PRODUCTS
·
2020
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IF 4.244
Tao Shen,Youqin Hu,Ruijia Hu,Wei Zhuang,Ming Li,Huanqing Niu,Hong Xu,Chenjie Zhu,Hanjie Ying
The continuous production of furfural using vaporization reactor as a continuous stirred tank reactor is reported in this study. 50 g L −1 xylose solution was continuous fed into the reactor and catalyzed by 2 wt% H 2 SO 4 at 433 K gave 82.3% average yield of furfural in 10 h of reaction time. Pulp prehydrolysate derives from prehydrolysis of wood biomass was further used as the raw material for the reaction, and the 78.6% average yield of furfural was achieved for 10 h. According to Aspen Plus simulation, 11.7 tons of saturated steam was required for the production of one ton of furfural under the present reaction system, which is almost half of the traditional one-step method.
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BIORESOURCE TECHNOLOGY
·
2020
·
IF 7.539
Peiwen Wu,Lianhua Li,Yongming Sun,Bing Song,Yun Yu,Huping Liu
This study is the first to integrate gamma-valerolactone/water (GVL/water) pretreatment with anaerobic digestion (AD) for biogas production and lignin nanoparticles (LNPs) synthesis. The hydrothermal treatment was conducted at 135 to 180 °C with GVL at 0 to 90%. After pretreatment, the compositions of hybrid pennisetum were changed with the removal of lignin, hemicellulose, and cellulose to different extent. Subsequent anaerobic digestion achieved a maximal specific methane yield of 228.00 ± 4.37 mL/g VS, compared with that at 165.11 ± 1.99 mL/g VS for the control. The highest actual methane yield (150 mL/g RM) was achieved by pretreatment with GVL/water (50/50) at 150 °C for 90 min. LNPs at 200 to 2000 nm were synthesized from the liquid waste with a yield at ~4 mg/mL. The mass balance of this integrated method was discussed. In general, the maximal valorisation of hybrid pennisetum was achieved based on a catalyst-free of GVL/water pretreatment.
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CARBOHYDRATE POLYMERS
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2020
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IF 7.182
Jun-Yi Yin,Lu-Yao Ma,Ming-Yong Xie,Shao-Ping Nie,Jian-Yong Wu
Konjac glucomannan (KGM) with a molecular weight (MW) of 823.4 kDa was partially degraded by endo-1,4-β-mannanase. Two hydrolyzed KGM fractions (KGM-M-1: 147.2 kDa and KGM-M-2: 21.5 kDa) were characterized and applied to the animal tests in comparison with the native KGM. After oral feeding to the mice, KGM-M-1 and KGM-M-2 significantly increased the levels of short chain fatty acids (SCFAs) in the colonic contents and the native KGM increased the SCFAs in the cecum. The more significant effect of the native KGM in the cecum may be attributable to its high viscosity, slowing down the movement of intestinal microflora through the cecum, while the lower MW KGM-M-1 and KGM-M-2 could move more easily through the colon to be fermented by colonic bacteria. This new finding may be useful for future research and development of low-MW KGM polysaccharides through enzyme hydrolysis for the desired gut health benefits.
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Food Science & Nutrition
·
2020
·Vol. 8
·期 7
·3212-3224
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IF 1.797
Ping Ma,Congyong Sun,Wenjing Li,Wenwen Deng,Michael Adu-Frimpong,Jiangnan Yu,Ximing Xu
Nonalcoholic fatty liver disease (NAFLD) is one of the prevalent and typical chronic liver diseases. In this study, we extracted a novel Angelica sinensis polysaccharide (ASP) with low molecular weight (MW) of 3.2 kDa through optimized “one-step” purification process. The major monosaccharide components of ASP were mannose, rhamnose, glucuronic acid, galactose, arabinose, and xylose with weight ratio of 0.23:0.17:14.41:0.39:1.68:0.87, respectively. Herein, “small” ASP could serve as an effective therapeutic option for NAFLD both in free fatty acid-induced L02 models and in high-fat diet-induced mice models. Results revealed that low MW ASP dose-dependently decreased TG, TC in vitro and TG, TC, ALT, HDL-C, and LDL-C in vivo. Oil Red O-positive area and Nile red fluorescence intensity decreased in ASP treatment groups both in vitro and in vivo which suggested ASP could reduce lipid accumulation and fatty regeneration. Hematoxylin–eosin staining results shown a decrease in hepatocytes ballooning indicating that ASP could ameliorate liver lipid degeneration. Briefly, a novel polysaccharide with low MW was successfully obtained which can prospectively act as NAFLD therapy.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
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IF 5.279
Songze Ke,Yanlei Yu,Qiaoli Xu,Bo Zhang,Sijia Wang,Weihua Jin,Bin Wei,Hong Wang
Saccharina japonica polysaccharide could modulate gut microbiota composition; however, the composition-activity relationship remains unclear, thus restricting its application. In the current study, we investigated the impact of eight different S. japonica polysaccharide fractions on the gut microbiota after day 2 and day 14 treatments on high-fat diet (HFD) feeding mice. The results showed that a 2 day HFD dramatically altered gut microbiota composition, and the additional 12 day HFD further strengthened the gut microbiota dysbiosis in the HFD group. LjA-1 and LjA-3 could partially alleviate the dysbiosis of gut microbiota composition and significantly alter gut microbiota function. Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera. Our findings reveal that gut microbiota homeostasis could be disordered by HFD at day 2 and provide insights into the quantitative composition-activity relationships of polysaccharides in regulating gut microbiota.
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GREEN CHEMISTRY
·
2021
·Vol. 23
·期 21
·8458-8467
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IF 10.182
Shanshan Xiong,Chao Luo,Zhihao Yu,Na Ji,Lingjun Zhu,Shurong Wang
The isomerization–dehydration cascade conversion on the saccharide platform is a pivotal route for biomass valorization, although it exhibits a specific reaction complexity that makes achieving an efficient conversion a highly technical challenge. The rational design of novel versatile solid acids is deemed to be the key to improving the cascade catalytic performance. In this paper, a dual-functional carbon-based solid acid catalyst (DFCSA) with tunable Brønsted/Lewis acid sites was developed for the cascade conversion of saccharides. The combined modification of H3PO4 and HNO3 on the carbon substrate contributed to the grafting of protonated moieties with a reasonably porous structure. With the aid of AlPO4, which has an orthorhombic crystal structure, the newly developed catalyst was able to achieve good performance for the subsequent hydrothermal conversion. Under optimal conditions, glucose and xylose were transformed with high product yields: 38.2 mol% levulinic acid and 69.7 mol% furfural, respectively. In addition, the catalyst displayed excellent hydrothermal stability, with only a slight degradation in activity after 10 cycles, which was ascribed mainly to the high thermal stability of AlPO4 and the firm grafting of the surface functional groups. As shown by the kinetic analysis, the DFSCA catalyst had a higher conversion rate and lower activation energy in the cascade conversion of xylose than that of glucose, leading to a better yield of furfural.
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FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Huipeng Liu,Xiaoyu Wei,Shuyu Zu,Xihuang Lin,Jinchuang Zhang,Aimin Shi,Qiang Wang,Ning He
Plant-derived neutral oligosaccharides and pectic-oligosaccharides (POS) have been suggested as prebiotics based on their degree of polymerization (DP) and structural complexity. In the present study, neutral oligosaccharides without galacturonic acid were produced from commercial apple pectin using an optimized concentration of pectinase and degradation time. The crude oligosaccharide solution was refined by membrane separation using a DEAE-Sepharose CL-6B column, a graphite carbon column and an Xbridge C18 column, which resulted in the neutral oligosaccharides with DP 3–13. The monosaccharide composition of oligosaccharides primarily included glucose, arabinose, galactose and xylose without galacturonic acid. The structure of the oligosaccharides was analyzed using mass spectrometry with an electrospray ionization source by UPLC on an HILIC column and 1 H NMR. The MS/MS spectra in positive ion mode and 1 H NMR showed a main oligosaccharide structure corresponding to the connection of arabinogalactan with Galβ1→4Galβ1→4Galβ1→4Galβ1→α3Ara f . The growth of the selected Lactobacillus . strains was improved by adding refined neutral oligosaccharides with DP = 3–13 compared with commercial inulin , and growth of Escherichia coli had not basically grown. Therefore, neutral oligosaccharides (DP = 3–13) can be considered effective prebiotics for potential application in functional food.
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BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Haichuan Zhang,Hui Zhang,Shenglong Tian,Shiyu Fu
In this work, near-complete conversion of lignocellulosic biomass and high products yields were achieved through catalytic transfer hydrogenolysis (CTH) in isopropanol using a heteropoly acid SiW 12 synergistic with Pd/C catalyst at a relatively mild condition. The performances of various heteropoly acids and catalytic conditions were extensively examined. The results confirmed that SiW 12 exhibited the highest activity on disrupting C–C linkages and C-O linkages than H 2 WO 4 , PW 12 , and PMo 12 . 34.91 wt% and 43.55 wt% monophenols were achieved for hydrogenolysis of bagasse and eucalyptus, respectively, at their optimal temperature for 5 h. Characterization studies on the lignin oil revealed that the notable structural changes were observed including the breaking of the side chain alkyl-aryl ether bonds and glycosidic bonds, while methoxyl groups were retained. Additionally, particle size of feedstock also has significant impact on the distribution and yields of the monophenols.
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NEW JOURNAL OF CHEMISTRY
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2021
·Vol. 45
·期 37
·17576-17583
·
IF 3.591
Lijun Xu,Lu Yang,Aihua Liu
One-pot synthesis of Mn3O4 nanorods is reported for the first time by the oxidation of manganese metal resulting from reducing Mn2+ by NaBH4 at room temperature. The resultant Mn3O4 nanorods were 10–70 nm in width and up to hundreds of nanometers in length, and were very stable in aqueous solution. The Mn3O4 nanorods can oxidize H2O2 under acidic conditions quickly accompanying their decomposition. Using 3,3′,5,5′-tetramethylbenzidine (TMB) as a competitive chromogenic substance, colorimetric methods were developed for the sensitive detection of H2O2 and glucose. The methods exhibited a linear range of 2–100 μM with a limit of detection (LOD) of 1.7 μM for detecting H2O2, and a linear range of 5–200 μM with a LOD of 4.4 μM for detecting glucose. The application in analyzing glucose in human serum samples demonstrates its practicability.
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ACS Sustainable Chemistry & Engineering
·
2021
·
IF 8.198
Yafeng Fan,Lianhua Li,Gaixiu Yang,Yongming Sun,Linsong He,Peiwen Wu,Chun Ho Lam,Bing Song
Gamma-valerolactone (GVL) assisted NaOH/water pretreatment for hybrid pennisetum delignification was first investigated. Contrary to the hypothesized results, the addition of GVL significantly suppressed the delignification and hemicellulose removal by mild alkaline pretreatment (37 °C for 24 h). An addition of GVL to GVL/NaOH molar ratio of 1/1 resulted in lignin removal at ∼23% compared with that at ∼75% without GVL. This suppression effect was further confirmed by scanning electron microscopy and Fourier-transform infrared spectroscopy. The extracted lignin was further characterized by two-dimensional heteronuclear single quantum coherence nuclear magnetic resonance spectroscopy, suggesting that the addition of GVL suppressed the decomposition of large lignin molecules by NaOH/water and the release of hemicellulose, resulting in extracted lignin with lower molecular weights and less hemicellulose. The decrease in the partial alkalinity (PA) of alkaline solutions caused by GVL addition was likely the main reason for the suppressed delignification, suggested by the good correlation between PA and delignification in both GVL/NaOH/water and NaOH/water (R2 = 0.967). This study suggested that solvent-assisted alkaline pretreatment can also result in an unwanted suppression effect, and the changes in PA could be referred to as an indicator while selecting organic solvents.
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危险品化学品经营许可证（带存储）