MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Guoli Chen,Ji-Na Hao,Yongsheng Li
5-Methylcytosine (5-mC), as one of the primary forms of DNA methylation, plays essential roles in a variety of biological activities. Its rapid and precise detection is therefore paramount for abnormal DNA methylation assessment, but still remains challenging. Herein, a highly luminescent and stable lanthanide-based metal-organic framework (MOF) probe, namely Eu-ZrMOF, is firstly designed for qualitative and quantitative analysis of 5-mC, wherein the organic building units of 1,3,5-benzenetricarboxylic acid (BTC) serve as both the energy donors and recognition molecules, and the modified Eu 3+ ions function as intense signal reporters. Significantly, the luminescence of the fabricated Eu-ZrMOF probe can be remarkably quenched by 5-mC via its inner filter effect on the BTC absorption, achieving high response to 5-mC in a very short time (20 s). Moreover, such an efficient, instantaneous response probe presents excellent specificity towards 5-mC among various coexisting species in urine, and high sensitivity with a linear detection range of 0–200 μM and limit of detection (LOD) of 0.18 μM, thereby enabling it to be applied in accurately quantifying 5-mC content in real urine samples with high recoveries (95.5–98.5 %) and low relative standard deviations (< 2.5 %). This study demonstrates a simple, fast and effective approach for sensitive identification of urinary 5-mC, prefiguring its great potential for the assessment of DNA methylation associated with genetic diseases and tumors.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 12
·830
·
IF 5.3
Hou Hongming,Long Jiajun,Li Xianglong
The chelation interaction between sodium lignosulfonate (SLS) and Cu 2+ is exploited to construct an enzyme-free creatinine sensing electrode (SLS-Cu/SPCE) via one-step electrodeposition on screen-printed carbon electrodes (SPCE). SLS chelation moderates Cu 2+ electro-reduction kinetics, resulting in finely structured Cu particles with a uniform and dense distribution. These Cu particles, with abundant unsaturated coordination sites, enhance creatinine adsorption and promote specific complexation with Cu 2+ , thereby increasing the oxidation current response for sensitive detection. The SLS-Cu/SPCE achieved a 0.12 µM detection limit, a 10–300 µM linear range, high reproducibility, and long-term stability over 28 days. Tests in human urine samples showed good agreement with enzymatic assays (relative error < 16%). The SLS-Cu 2+ coordination strategy offers a novel approach for optimizing Cu-based enzyme-free electrochemical creatinine sensors. Graphical A sodium lignosulfonate-Cu 2+ chelation strategy produces refined copper particles on SPCE with abundant unsaturated sites. These sites enhance creatinine adsorption and Cu 2+ complexation, enabling highly sensitive non-enzymatic detection (0.12 μM LOD, 10-300 μM range) with strong anti-interference and excellent stability.
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BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Changyun Quan,Liping Wen,Minghui Yang,Binjie Xu
Given the metabolic interplay between glucose and uric acid, individuals should proactively monitor and be alert of elevated levels of both substances. For the first time, this paper describes a novel design of electrochemical biosensor based on screen-printed electrode of a single channel but capable of simultaneously measuring blood glucose and uric acid concentrations. Experimental results demonstrated that this single-channel biosensor exhibits good performance towards glucose and uric acid, such as broad linear range, good reproducibility, good stability, and strong resistance against interference. For spiked recovery experiments on human venous blood samples, the glucose recovery rate ranged from 95.9 % to 103.2 %, while the uric acid recovery rate ranged from 99.7 % to 104.9 %. And the relative standard deviation for both parameters was less than 5 %, indicating that this biosensor holds promising prospects for commercial application. This is truly the first time that enable simultaneous detection of glucose and uric acid concentrations using just one drop of blood and a single test strip. It reduces the number of blood draws required, lowers analysis costs, and significantly improves screening efficiency for individuals with diabetes and hyperuricemia.
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ACS Nano
·
2025
·
IF 16
Xianyou Sun,Zihan Jin,Shaoxiang Zhang,Peng Lin,Xueming Zhang,Jiaying Sun,Yanchi Zhang,Yong Zhou,Yanjie Hu,Zhuoru Huang,Jiahao Hu,Shichao Tian,Guangqing Ren,Ping Wang,Liubing Kong,Hao Wan
Urine, abundant in disease-related biomarkers, offers advantages such as easy collection and noninvasiveness. Portable biochemical sensors are ideal for efficient on-site applications due to their flexibility and accessibility. However, existing sensors face challenges in complex sample operations and limited sensor renewal, hindering large-scale and convenient urine analysis. This study presents the “lab on pipette” (LOP) concept to address these issues, enabling efficient on-site urine analysis. The LOP system integrates a multifunctional pipette tip and a miniaturized system, facilitating the detection of multiple biomarkers in urine. Laser-induced graphene (LIG) technology fabricates an electrochemical electrode array on flexible polyimide, and custom-designed electrical interfaces ensure quick plug-and-play connections. Gold nano dendrites, Prussian blue (PB), and nickel hexacyanoferrate (NiHCF) form a multilayer functional interface, enabling highly sensitive detection of pH, hydrogen peroxide, glucose, and ascorbic acid with pH compensation. NiHCF doping enhances PB stability, while nanoalumina-doped porous enzyme membranes improve sensor repeatability and reliability. Gold nano dendrites anchor the enzyme membrane on curved surfaces, ensuring robust performance. The LOP achieves detection limits of 7.19 and 8.57 μM for glucose and ascorbic acid, respectively. Its low cost, disposability, portability, and reliability highlight its potential for on-site biochemical detection and personalized health monitoring.
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ACS Nano
·
2025
·
IF 16
Wei Deng,Mimi Sun,Mengzhu Cao,Chong-Bo Ma,Xiangjie Bo,Jing Bai,Ming Zhou
Wearable wound exudate sensors hold great promise for providing dynamic measurements of valuable biomarkers. However, no existing sensors are able to achieve the fully integrated, skin-on, and dynamic detection of raw wound exudate oxygen (O2), which is closely related to wound conditions and also essential for wound healing. Here, we report a fully integrated wearable biomimetic microfluidic wound tracker, capable of skin-on biomimetic microfluidic wound exudate sampling, dynamic monitoring of wound exudate O2 in addition to wound exudate uric acid, lactate, pH, and temperature, and wireless control through the seamless integration of specially designed microfluidic, sensing, and electronic modules. We test the performance of the device in both bacterium-inoculated and uninoculated wounds using mouse models. We further assess its potential for wound management in the healing process of infected diabetic mouse wounds through controlled experiments related to local hyperbaric O2 treatment.
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FASEB JOURNAL
·
2025
·Vol. 39
·期 23
·e71312
·
IF 4.2
Tong Chen,Li Lin,Haiwei Chen,Songxiao Zou,Kaixin Lan,Hehuan Ruan,Yizhong Tu,Yu He,Ronghai Deng,Chenglin Wu,Gang Huang,Shicong Yang,Qin Wang,Liang Tao,Guodong Chen,Xiaoshun He,Shuangjin Yu,Jiang Qiu
Although normothermic machine perfusion (NMP) has been employed in clinical transplantation, the efficient and standardized long-term NMP strategies for small animals remain to be explored, which may significantly facilitate fundamental biomedical research and drug evaluation. We established a straightforward rat kidney ex vivo NMP system and explored the efficacy of different perfusates by comparing various parameters, including the perfusion and metabolic parameters, renal function, histological features, acute renal injury score, and inflammation. Additionally, we implanted the kidneys after NMP and demonstrated the effectiveness and safety by comparing the survival of recipients, acute renal injury, and graft function. The kidneys were able to produce urine during NMP, and the groups with fetal bovine serum (FBS) exhibited higher urine output compared to those supplemented with bovine serum albumin (BSA). Nonetheless, the BSA group displayed more stable perfusion resistance, enhanced oxygen consumption efficacy, superior renal tubule function in electrolyte reabsorption and excretion, and reduced vessel injury and tissue inflammation. All grafts were successfully transplanted, and the BSA group showed slighter injury than FBS on postoperative 1 and 7 days and was not significantly different from the cold storage group. A supporting kidney function in vitro was achieved using the optimized NMP method with the perfusate consisting of rat peripheral blood, RPMI-1640, and BSA. This novel NMP strategy in small animal models may benefit basic studies related to organ preservation, assessment, and intervention in the future. Graphical We established a rat kidney ex vivo normothermic machine perfusion system and evaluated different perfusates by assessing perfusion/metabolic parameters, renal function, histology, and inflammation. Implanting perfused kidneys, we confirmed efficacy and safety via recipient survival and graft function. The perfusate with rat peripheral blood, RPMI-1640, and BSA showed superior renal function and minimal organ injury, promising for organ preservation, assessment, and intervention studies.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Yingfei Hui,Hao Guo,Mingyue Wang,Liping Peng,Henglong Ren,Borong Ren,Wu Yang
Detection of sialic acid (SA) is crucial for the early diagnosis of ovarian cancer. In this study, we present the construction of a novel boronic acid functionalized covalent organic framework (TMTA-PBA-COF) for the first time for the accurate detection of SA. By combining the excellent luminescent properties of TMTA-COF with the specific binding capability of the boronic acid moiety to the cis-diol moiety in SA, the constructed fluorescent sensor achieved a sensitive and selective detection of SA in human serum within the linear range of 1–500 μM with a low detection limit of 0.45 μM. Crucially, we also established an intelligent back propagation neural network model to process the fluorescence sensing results, enabling accurate identification of SA concentrations through deep machine learning without cumbersome data processing. In addition, the practical application of TMTA-PBA-COF is extended to the visual identification of latent fingerprints. This work provides an intelligent and efficient analytical method for the early diagnosis of ovarian cancer and expands the multifunctional application of the material.
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TALANTA
·
2025
·
IF 6.1
Ping Gao,Yan Liu,Jing Sun,Qi Chang,Leijiao Li,Zhongmin Su
Continuous and quantitative measurement of toxin biomarkers in biological fluids constitutes a significant advancement in the proactive management of chronic kidney diseases (CKD). Although sweat analysis presents a promising non-invasive strategy, conventional detection methods suffer from inherent limitations, including low sensitivity, poor specificity, high cost, and inadequate long-term durability. Herein, we constructed a novel molecularly imprinted portable biosensor that can simultaneously detect three critical biomarkers (urea, creatinine, and uric acid) for CKD in human sweat. The sensor was fabricated on flexible polyethylene terephthalate (PET) film using screen-printing technology to form a five-electrode array consisting of one counter electrode, one reference electrode and three working electrodes. The ternary composite electrodes (NiCoMOF/MWCNTs/NCDs) were designed as sensing substrate, the porous structure derived from metal-organic frameworks provides abundant active sites for analyte sensing and in synergy with the conductivity of MWCNTs and NCDs, endows the ternary composite electrode with a high electrochemically active surface area (A) of 0.062 cm 2 , a rapid electron transfer rate ( k 0 ) of 6.685 × 10 −3 cm s −1 , and a low electron transfer resistance of 52.79 Ω, significantly enhancing its electrochemical properties (Bare carbon, A: 0.0161 cm 2 , k 0 : 0.613 × 10 −3 cm s −1 ). Molecular imprinting technology generates highly specific recognition cavities with enhanced rebinding efficiency by removing template molecules from each working electrode. The portable sensing platform exhibits sensitivities of 6.2 μA mM −1 cm −2 , 134 nA μM −1 cm −2 , and 1870 nA μM −1 cm −2 for the detection of urea, creatinine, and uric acid, respectively, with detection limits of 0.048 mM, 0.032 μM, and 0.024 μM. The linear ranges encompass the physiological concentrations of these analytes in sweat, with corresponding correlation coefficients (R 2 ) of 0.9959, 0.9952, and 0.9981. The non-enzymatic mechanism ensures that the signal retention rate remains above 95.8 % after 60 days of storage. The results from various scenario tests demonstrated that the sensing system successfully achieved synchronous dynamic monitoring of three biomarkers in sweat samples. Hence, this work shows high development prospects for a continuous, convenient, non-invasive sensing platform for point-of-care diagnosis and personalized management of chronic kidney diseases.
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Journal of Materials Chemistry C
·
2025
·
IF 5.1
Qing Liu,Tigang Ning,Jing Li,Zhouyi Hu,Lanju Liang,Haiyun Yao,Xin Yan,Yongzhen Chen,Qingyi Wang,Shanghui Guan
Terahertz (THz) fingerprint metasensing is an effective method for identifying biological substances, offering advantages such as high efficiency and non-destructiveness. Currently, one important approach to achieving THz fingerprint spectrum sensing is the design of THz metasurface (MS) sensors with broad spectral coverage and multiple resonant peaks. However, there is still exhibit potential for further enhancement in the sensitivity, selectivity, and detection limits of THz sensors. Gallium nitride (GaN), with its exceptional optoelectronic properties such as high carrier concentration, high mobility, and excellent chemical stability, can be integrated with THz MSs to create high-performance sensors. In this study, we propose a THz MS biosensor coated with GaN. Since different analytes exhibit distinct absorption characteristics in the THz range, preliminary identification of various substances can be achieved by analyzing differences in the trends of spectral amplitude changes. Experimental results demonstrate that the proposed metasensor achieves a detection limit of 1 μg/mL for bovine serum albumin (BSA) and 10 pg/mL for creatinine. Through radar chart analysis, the transmission spectra of the metasensor show significant differences when detecting creatinine and BSA, indicating its capability to effectively identify different analytes. The GaN metasensor developed in this study provides a highly sensitive and broadly spectrally covered solution for THz fingerprint spectrum analysis, which holds potential applications in assessing kidney function and health status.
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ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Zhaoxian Chen,Shiyi Zhou,Li Wang,Yingzhou Huang
Surface-enhanced Raman spectroscopy (SERS) and spoof surface plasmon polaritons (spoof-SPPs) are two electromagnetic detection techniques that have received significant attention in recent years. In this work, a novel spoof-SPPs and SERS dual-mode detection of multiple biomarkers in sweat is introduced, which is based on an Archimedean spiral Cu patch integrated with a Ag nanocoral surface. As a spoof-SPPs sensor, the reflection spectrum of the Archimedean spiral Cu patch exhibits high sensitivity to target biomarkers, which also reveals significant shortcomings in the detection of multiple biomarkers with various proportions. With the help of Ag nanocoral surface on Cu patch, SERS spectrum of multiple biomarkers is also obtained on the same patch, which provides supplementary information about the proportions based on the intensities of their different characteristic Raman peaks. With the total amount of multiple biomarkers as the same, our data demonstrate that the variation of volume ratios (7:3, 5:5, and 3:7) between creatinine and urea in sweat at 100 nM could be accurately identified by spoof-SPPs and SERS spectra. Therefore, our novel spoof-SPPs and SERS dual-mode detection overcomes the deficiency of spoof-SPPs sensing on multiple targets and SERS sensing on quantitative analysis, which exhibits huge application prospective in accurate and reliable sensing on complex biochemical systems.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Feng-Feng Zhang,Mei-Juan Wu,Zi-Wei Yu,Yi-Lan Lin,Pin-Fang Huang,Meng-Meng Liu
Methotrexate (MTX) has definite dose-response relationship, in which high level MTX in serum leads to severe adverse events such as hepatotoxicity and myelosuppression. Therapeutic drug monitoring (TDM) of MTX helps to improve clinical outcomes and reduce toxic side effects. Nevertheless, standard methods are restricted by time-consumption, high cost and multiple repetitive sampling. Herein, a point-of-care testing (POCT) sensor was integrated with physiologically based pharmacokinetic (PBPK) model for on-site TDM of MTX to obtain patient-specific concentration-time ( C-T ) profiles, guiding leucovorin rescue and managing toxicities of high dose MTX. The sensor employed graphene oxide (GO)-ionic liquid (IL) modified screen-printed carbon electrodes in conjunction with a portable electrochemical workstation to achieve fast and accurate MTX detection in human serum via cyclic voltammetry, exhibiting two distinct linear dynamic ranges (0.1 nM-100 μM and 100 μM-3 mM) with a detection limit of 1.3 pM. The GO-IL composite enables selective MTX detection through different oxidation potentials, π-π stacking with MTX's pteridine ring, and charge/hydrophobicity-guided exclusion of interferents, achieving discrimination against key interferents including 7-OH MTX. Pearson correlation analysis and Bland-Altman consistency analysis demonstrated that MTX concentrations of 86 clinical samples measured by the sensor were consistent with that of immunoassay. Innovatively, PBPK model of MTX was developed to effectively process the complex MTX concentration data to obtain personalized C-T profiles of each patient. The individual PK parameters like maximum concentration and area under the curve can be easily acquired through C-T profiles, allowing for rapid clinical decision making. The MTX-PBPK model displayed reliable predictive accuracy of PK parameters within 2.0-fold-error. The results demonstrate that the POCT sensor combined with PBPK model is promising to be used for on-site TDM of MTX, promoting the development of individualized medication.
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TALANTA
·
2024
·
IF 5.6
Lichen Gou,Ling Li,Siqi Wei,Yunfei Tian,Xiandeng Hou,Li Wu
Herein, the surface-enhanced Raman scattering (SERS) platform was combined with an azo coupling reaction and an aluminum alloy covered with a hydrophobic layer of praseodymium oxide and stearic acid complexes for the detection of histamine. The praseodymium oxide on aluminum alloy was successfully synthesized by the rare-earth-salt-solution boiling bath method and modified by stearic acid. Its surface exhibits a water contact angle (WCA) of 125.0°. Through the azo derivatization reaction with 3-amino-5-mercapto-1,2,4-triazole (AMTA) diazonium salts, histamine can be converted into the derivatization product with higher Raman activity. The mixture of the derivatization product and β-cyclodextrin-modified Ag nanoparticles (β-CD-AgNPs) were dropped onto the surface of an aluminum alloy covered with a hydrophobic layer of praseodymium oxide and stearic acid complexes, and dried for SERS measurement. The intensity ratio between the SERS peaks at 1246 cm −1 and 1104 cm −1 (I 1246 /I 1104 ) of the derivatization product was used for the quantification of histamine. Under the selected conditions, the limit of detection (LOD) and the limit of quantification (LOQ) for this method were 7.2 nM (S/N = 3) and 24 nM (S/N = 10), respectively. The relative standard deviation (RSD) of this method for the determination of 1 μM histamine was 6.1 % (n = 20). The method was also successfully used for the determination of histamine in fish samples with recoveries ranging from 92 % to 111 %. The present method is simple, sensitive, reliable, and may provide a new approach for preparing the composite hydrophobic layer that can enhance SERS signals through hydrophobic condensation effect. Meanwhile, it may have a promising future in the determination of small molecular compounds containing an imidazole ring.
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