JOURNAL OF SEPARATION SCIENCE
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2011
·Vol. 34
·期 22
·3115-3122
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IF 2.631
Xiaojun Dai,Yuan He,Yinmao Wei,Bolin Gong
A one-step procedure based on surface-initiated atom transfer radical polymerization (SI-ATRP) to hydrophilize monodisperse poly(chloromethylstyrene-co-divinylbenzene) beads has been presented in this work, using 2-hydroxyl-3-[4-(hydroxymethyl)-1 H -1,2,3-triazol-1-yl]propyl 2-methylacrylate (HTMA) as a monomer. The chain length of the grafted poly(HTMA) was controlled via varying the ratio of HTMA to initiator on the surface of the beads. When using the grafted beads as a stationary phase in hydrophilic interaction chromatography (HILIC), good resolution for nucleobases/nucleosides was obtained with acetonitrile aqueous solution as an eluent; while for phenolic acids and glycosides, they could be eluted and separated in the presence of TFA. The retention time of the solutes increased with the amount of the grafted HTMA. The retention mechanisms of solutes were investigated by the effects of mobile phase composition and buffer pH on the retention of solutes. The results illustrated that the retention behaviors of the tested solutes were dominated by hydrogen bonding interaction and electrostatic interaction. From the chemical structure of the ligands, the modified beads could not only be used as a stationary phase in HILIC, but also act as a useful building block to develop new stationary phases for other chromatographic modes such as affinity media.
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JOURNAL OF CHROMATOGRAPHY A
·
2011
·
IF 4.194
Hongyue Guo,Renhua Liu,Jinjin Yang,Bingcheng Yang,Xinmiao Liang,Changhu Chu
A novel type of zwitterionic HILIC stationary phase was prepared by covalently bonding the l -azido lysine on silica gel via click chemistry. The key intermediate azido lysine was synthesized by transformation the amino group in l -Boc-lysine to corresponding azido group and subsequent removal of the N-protected group (Boc). Finally, the azido lysine was covalently bonded to silica beads by click chemistry to get click lysine. Its structure was confirmed by FT-IR and elemental analysis. The new stationary phase showed good HILIC characteristics, when it was applied to separate polar and hydrophilic compounds, such as organic acids, cephalosporins and carbapenems. Compared with the commercial stationary phases, such as Atlantics HILIC and ZIC-HILIC, click lysine displayed better or similar chromatographic behaviors.
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TALANTA
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2017
·
IF 4.162
Pingxiu Tang,Zilin Chen
Hypercrosslinked polymers (HCPs) are currently receiving great attention due to their unique characteristics and potential uses in diverse areas. However, the field of HCPs for open-tubular capillary electrochromatography (OT-CEC) separations has not been explored. Here, a knitted aromatic polymer (KAP) was in-situ grown on the inner wall of the capillary column for OT-CEC for the first time. The silylating reagent containing phenyl was served as monomer , immobilized on the inner wall of the capillary column, and then KAPs-modified capillary column was prepared through in-situ hypercrosslinking reaction. The surface structure and morphology of KAPs-modified capillary column was characterized by Fourier transform infrared spectra (FT-IR) and scanning electron microscopy (SEM). The prepared capillary columns showed good separation performance for neutral compounds, small biomolecules, such as nucleosides , amino acids , small peptides, and non-steroidal anti-inflammatory drugs, sulfa drugs. In addition, the KAPs-modified capillaries showed good reproducibility, with relative standard deviations for intra-day, inter-day and column-to-column runs less than 1.59%, 2.55%, and 5.19% respectively. The strategy of in-situ immobilization of KAPs provides a new approach for the application of the material in the analytical fields.
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ANALYTICA CHIMICA ACTA
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2024
·
IF 5.7
Xiang Wang,Xingrui Wang,Jiajia Wu,Jiayu Yu,Hanlin Zeng,Hanqi Yang,Huanjun Peng,Guangming Zhou,Jingdong Peng
Background Food safety has become an essential aspect of public concern and there are lots of detection means. Liquid chromatography plays a dominating role in food safety inspection because of its high separation efficiency and reproducibility. However, with the increasing complexity of real samples and monitoring requirements, conventional single-mode chromatography would require frequent column replacement and cannot separate different kinds of analytes on a single column simultaneously, which is costly and time-consuming. There is a great need for fabricating mixed-mode stationary phases and validating the feasibility of employing mixed-mode stationary phases for food safety inspection. Results This work fabricated multifunctional stationary phases for liquid chromatography to determine diverse food additives under the mixed mode of RPLC/HILIC/IEC. Two dicationic ionic liquid silanes were synthesized and bonded onto the silica gel surface. The functionalized silica was characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and elemental analysis. Both columns provide satisfactory separation performance towards 6 hydrophilic nucleosides, 4 hydrophobic polycyclic aromatic hydrocarbons, and 5 anions. Great repeatability of retention (RSD <0.1 %) and column efficiency (100330 plate/m) were obtained. Thermomechanical analysis and linear solvation energy relationship investigated the retention mechanism. Finally, the better in two prepared columns was employed to separate and determine the contents of NO 2 − and NO 3 − in vegetables(highest 4906 mg kg −1 NO 3 − in spinach), preservatives in bottled beverages (180.8 mg kg −1 sodium benzoate in soft drink), and melamine in milk with satisfactory performance and recovery rates ranging from 96.4 % to 105.6 %. Significance This work developed a novel scheme for preparing mixed-mode stationary phases by dicationic ionic liquid which provides great separation selectivity. Most importantly, this work proved the superiority of employing mixed-mode stationary phases for food safety inspection, which might avoid high-cost and frequent changes of columns and chromatography systems in the near future.
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Science China-Chemistry
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2025
·
IF 9.7
Chen Qianqian,Xu Haoxing,Zhao Bingyue,Patehebieke Yeersen,Zhao Zerun,Wang Xiao
The selective nucleoside synthesis through direct condensation between nucleobase and unactivated sugar in aqueous media has long been a formidable obstacle for the early chemical transformations hypothesized to construct the RNA World, due to product complexity and the low selectivity of the desired isomer. The preference of β-furanoside, the extant RNA component, has never been documented. Here we report an aqueous ribosylation mediated by divalent transition-metal ions, which unprecedentedly enhances the selectivity of furanosyl ribonucleosides as opposed to the traditionally predominating pyranosyl ones, with the furanoside/pyranoside ratio reaching up to 9/1. Furthermore, β-furanoside stands out for the first time as the most prevalent isomer in the presence of zinc and ammonium salts. The mechanism of this selectivity is elucidated with density functional theory calculations. The selectivity for β-furanoside can be further elevated via the adsorption on a zinc mineral. These findings exhibit as the first example that the selection of nucleosides with the desired, canonical configuration might be completed in the early stage of the RNA-forming process as opposed to later evolution, promoted by simple and widespread transition-metal minerals. The study may also find its value in synthetic chemistry as a guideline for developing a sustainable and selective glycosylation process.
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JOURNAL OF CHROMATOGRAPHY A
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2024
·
IF 3.8
Xiao-Dong Cheng,Zheng Zhang,Xue-xin Dai,Yun-Ping Li
A 3 μm undecylenic acid-functionalized stationary phase (UAS) was prepared for the separation of nucleosides and nucleobases using per aqueous liquid chromatography (PALC) and hydrophilic interaction liquid chromatography (HILIC). The retention behaviors of nucleosides and nucleobases in PALC and HILIC modes were explored by adjusting parameters such as water content, buffer concentration, pH of the mobile phase and column temperature. The experimental data and separation chromatogram demonstrated that PALC could provide retention comparable to that of HILIC for nucleosides and nucleobases. Comparative studies using diluted adenosine solutions evaluated theoretical plates and peak shape for the same retention factors (between 0.25 and 5.0) in PALC and HILIC. There was no buffer component in the mobile phases used to operate the comparisons. HILIC mode is more efficient for adenosine than PALC mode at low retention factors. It's the exact opposite phenomenon for high retention factors. It is proposed that the mass transfer of adenosine between the UAS, the water-rich layer and the ACN-rich mobile phase in HILIC is relatively slow. Given the significant use of toxic ACN in HILIC, PALC emerges as a safer and more effective alternative for separating nucleosides and nucleobases.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Jiana Du,Yan Liu,Lizi Li,Qin Yin,Dehong Yu,Pei He,Na Wang,Ruiying Yuan,Zhujun Yin,Yanbei Tu,Yanfang Li
Hyperuricemia (HUA) is a metabolic disorder characterized by increased serum uric acid (UA) levels. Here, we showed that pentagalloyl glucose (PGG), a well-known natural polyphenol, significantly decreased UA levels both in potassium oxonate/hypoxanthine-induced HUA mice and adenosine-induced hepatocytes. Mechanistically, the UA-lowering effect of PGG was attributed to the suppression of UA formation by inhibiting xanthine oxidase (XOD) activity and expression. PGG acted as a reversible mixed-type inhibitor of XOD (IC50 = 3.92 ± 0.06 μM), and its binding was driven primarily by hydrogen bonding and van der Waals forces. Structural analyses revealed PGG occupied the catalytic center of XOD, obstructing xanthine binding and inducing conformational changes. Integrated kinetics, isothermal titration calorimetry, multispectroscopic investigations, and computer simulations confirmed these interactions. These findings first comprehensively suggest the anti-HUA effect of natural XOD inhibitor PGG and highlight the promising potential of PGG as a functional food or drug candidate for treating HUA-related diseases.
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DRUG AND CHEMICAL TOXICOLOGY
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2025
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IF 1.9
Yongxiao Huang,Caina Jiang,Fangyao Li,Xianli Ma
As a commonly used flame retardant in numerous products, it is inevitable that tris(2-chloroethyl)phosphate (TCEP) is released into the surrounding environment during its use. This process gives rise to potential environmental concerns that must be addressed. In recent years, there has been significant interest in the role of TCEP in the development of hyperuricemia (HUA). However, the specific mechanisms by which TCEP contributes to this condition remain to be fully elucidated. In this study, we have employed a combination of network toxicology and in vitro experiments to investigate the potential effects of TCEP on HUA and its mechanism of action. Through systematic analysis of GeneCards, OMIM, Swiss Target Prediction, and CHEMBL databases, a total of 242 TCEP-induced HUA targets were identified. Utilizing the STRING and DAVID databases further elucidated the core targets and associated signaling pathways of TCEP in relation to HUA. The molecular docking assay results demonstrated that TCEP exhibits binding activity with the selected targets. In vitro, our findings revealed that TCEP exacerbates HUA by amplifying the inflammatory response and upregulating the mRNA expression levels of GLUT9 and URAT1. The present study provides a new perspective and theoretical basis for the in-depth understanding of the molecular mechanism of TCEP affecting HUA, and helps to further understand the pathogenesis of HUA and the role of environmental factors.
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FOOD CHEMISTRY
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2025
·
IF 9.8
Shuang Cui,Bo Wang,Zheng Zhou,Wei Yang,Lei Qin,Xuhui Huang
The absence of reliable identification technologies limits the authentication of geographical origin and growth conditions in large yellow croakers (LYCs). This study addressed this issue by analyzing odor and taste-active compounds to enable effective differentiation and quality evaluation. The findings revealed that inorganic sulfides, alcohols, aldehydes, ketones, nitrogen-containing oxides, and methyl compounds served as potential odor markers for the rapid discrimination of LYC meat from various origins, with 3-methyl-1-butanol and hexanal identified as key distinguishing odor compounds. Umami-enhancing compounds such as inosine-5'monophosphate (IMP) and trimethylamine N-oxide (TMAO), sourness-contributing lactic acid, and bitterness-associated taurine (Tau) were identified as critical taste-active markers for the differentiation of LYC varieties. IMP was the primary differential compound between wild large yellow croakers (WLYCs) and farmed large yellow croakers (FLYCs). This study provides guidance for consumer preferences regarding different LYC varieties and facilitates the efficient alignment of regional characteristics with processing requirements in the food industry.
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BMC PLANT BIOLOGY
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2025
·Vol. 25
·期 1
·1-17
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IF 4.8
Deng Dehai,Qin Yichao,Lin Xiuying,Chu Meng,Lv Daizhu,Lin Huan
Background The oil palm ( Elaeis guineensis ) is a crucial agricultural commodity, yielding the highest oil output among oil-bearing crops. Despite its significance, productivity challenges persist due to genetic and environmental factors. This study breaks new ground by mapping tRNA modifications in oil palm, exploring their roles during fruit ripening, an area not extensively studied in non-model crops. Results Utilizing advanced RNA mass spectrometry techniques, we identified 48 distinct tRNA modifications across 88 sites, alongside 164 genes associated with tRNA modifying enzymes. This comprehensive mapping reveals the decreasing nature of most tRNA modifications during fruit development, except for adenosine 2’-O methylation (Am). It hints at a gradual weakening of protein translation quality control and highlights a unique role for Am during fruit ripening. Additionally, lipidomic analysis tracked 674 lipids in oil palm fruits, indicating a correlation between tRNA modifications and the accumulation of specific lipids. Conclusions This study mapped tRNA modifications in oil palm for the first time and showed the diversity of dynamic changes in tRNA modifications as the fruits develop.
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Probiotics and Antimicrobial Proteins
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2025
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IF 4.4
Huang Fang,Lu Yun-hui,Xiong Jie,Zeng Xin-an,Huang Yan-yan,Wang Lang-hong
The escalating global burden of hyperuricemia, driven by modern dietary patterns and sedentary lifestyles, has prompted urgent exploration of novel therapeutic strategies, with particular emphasis on developing targeted probiotic interventions as a potential alternative to conventional pharmacological approaches. This study successfully isolated Limosilactobacillus fermentum FOSU YHD19 from fermented foods, demonstrating remarkable in vitro uric acid reduction of 12.90% along with high-level degradation of nucleosides. A comprehensive safety evaluation confirmed the absence of harmful enzymatic activities (including nitroreductase, decarboxylase and tryptophanase), hemolytic properties, or antibiotic resistance. The strain exhibited strong stress tolerance, surviving in acidic conditions (pH 2.0), with exposure to gastric juice, and when in the presence of 0.1% bile salts. Additionally, YHD19 demonstrated potent antibacterial activity against multiple pathogenic strains, including E coli , S. aureus , L. monocytogenes , and S. enteritidis . Genomic analysis revealed three nucleoside hydrolases and six transporter systems that collectively facilitate the hypouricemic activity. By ruling out the presence of key uric acid-synthesizing enzymes (xanthine dehydrogenase/oxidase and guanine deaminase), we can attribute the hypouricemic effect primarily to a nucleoside-scavenging mechanism. In addition, the genome harbors 91 stress-responsive genes implicated in acid/base adaptation, thermal shock response, and oxidative defense mechanisms, underscoring the strain’s resilience under gastrointestinal stresses. These findings position YHD19 as a safe and effective probiotic candidate for functional foods aimed at managing hyperuricemia, supporting its safety profile for potential probiotic applications.
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Journal of Analysis and Testing
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2025
·
IF 7
Chen Jiajing,Lin Juan,Ouyang Dan,Lin Zian
Metabolomics, based on laser desorption/ionization mass spectrometry (LDI-MS), has been successfully utilized for the high-throughput metabolic analysis of biofluids. This technique shows great promises as a powerful tool for disease screening. Although various nanomaterials have been developed as alternatives to traditional organic matrices in LDI-MS, the signals of most metabolites are limited due to their low abundance in complex biofluids. Herein, four hydroxyl-functionalized covalent organic frameworks (COFs) were designed as substrates for LDI-MS, named COF-OH- n ( n = 0–3, demonstrating the number of hydroxyl units). Since hydroxyl groups can facilitate proton transfer and promote the ionization process effectively, hydroxyl-rich COF-OH-2 enabled sensitive and efficient LDI-MS analysis of serum directly, resulting in highly reproducible serum metabolic profiles (SMPs). An orthogonal partial least squares discriminant analysis (OPLS-DA) model was successfully used to distinguish 30 gout patients from 38 healthy volunteers, with an area under the curve (AUC) of 0.974. Furthermore, the roles of 12 biomarker candidates, which were characterised following statistical analysis, in the metabolic pathway of gout were initially explored. Furthermore, the roles of 12 biomarker candidates, which were characterized following statistical analysis, in the metabolic pathway of gout were initially explored. This work demonstrates the potential of COF-OH-2-assisted LDI-MS in clinical metabolic analysis and offers a rational design strategy for advanced LDI-MS substrates.
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