TOXICOLOGY AND APPLIED PHARMACOLOGY
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2025
·
IF 3.4
Sangping Li,Shule Tan,Xiangsheng Tian,Yiquan Ou,Shujuan Hu,Weichao Zhao,Dingxin Long
This study investigated the protective effects of resveratrol (Res) against tri-o-cresyl phosphate (TOCP)-induced spinal cord neurotoxicity in adult hens, focusing on its modulation of ferroptosis via the p62/Keap1/Nrf2 pathway. Resveratrol is a classical neuroprotective compound with antioxidant properties and the ability to activate Nrf2. Adult hens were assigned to six groups: Control, TOCP, Ferrostatin-1 (Fer-1), Ferrostatin-1 + TOCP, Resveratrol, and Resveratrol + TOCP. Spinal cord tissues were analyzed using behavioral OPIDN scoring, histology (hematoxylin–eosin and Nissl staining), biochemical assays, and Western blotting for ferroptosis- and p62/Keap1/Nrf2-related proteins. TOCP exposure induced severe ultrastructural damage, including myelin sheath disruption and neuronal degeneration, along with increased malondialdehyde (MDA) and Fe 2+ levels and decreased glutathione (GSH) and superoxide dismutase (SOD) activity. Western blot analysis demonstrated upregulation of NCOA4, ACSL4, Nrf2, P62, and LC3 II, with downregulation of GPX4, SLC7A11, FTH1, and Keap1. Resveratrol treatment significantly attenuated these molecular, biochemical, and histopathological alterations, mitigating oxidative stress and ferroptotic changes.
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高影响因子
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
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2025
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IF 3.3
Zang Yanqing,Chuang Yingying,Wang Changyuan,Cao Yang
The aim of this study was to investigate the optimal process conditions for the preparation of white quinoa polysaccharides and the bioactivity of white quinoa polysaccharides in vitro. This study used white quinoa as raw material and utilized ultrasound-assisted extraction to extract polysaccharides from white quinoa. The optimal extraction technology for white quinoa polysaccharides (WQP) was studied through response surface methodology (RSM) experiments. In addition, preliminary characterization of WQP and determination of their physicochemical properties were conducted. Determined the content of nutrients in WQP. The hypoglycemic, antioxidant, and lipid-lowering effects of WQP were evaluated by different in vitro experiments. The results showed that the yield of WQP was 2.11% under the optimal process. WQP inhibited α-glucosidase and α-amylase by 60.85% and 51.30%, respectively. The scavenging rates of ▪OH, DPPH, ABTS, and ▪O 2 − radicals were 48.36%, 67.17%, 74.87%, and 61.26% respectively. In addition, WQP has some choline-binding ability. It also inhibited cholesterol lipase and pancreatic lipase by 49.76% and 80.57% respectively. The present study demonstrated the glucose-lowering, antioxidative and lipid-lowering effects of white quinoa polysaccharide in vitro.
研究领域:
食品科学
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生物化学
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药理学
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营养学
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生物技术
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Food & Function
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2025
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IF 5.4
Junzhu Li,Xin Feng,Yizhi Jing,Haidong Qian,Yun Ji,Yanling Hao,Zhengyuan Zhai
Infectious enteritis is a chronic inflammatory condition of the intestine, mainly characterized by immune cell infiltration and gut microbiota dysbiosis. Resveratrol, a natural polyphenol compound from grapes, berries and peanuts, is known for its antioxidant and anti-inflammatory properties. This study investigated the effects of resveratrol in a murine model of Citrobacter rodentium-induced colitis. Resveratrol ameliorated disease symptoms, body weight loss, colon shortening, and increased intestinal permeability. Histological and immunofluorescence analyses revealed that resveratrol improved intestinal barrier integrity by increasing goblet cells, upregulating MUC-2 expression, reducing crypt hyperplasia, and suppressing epithelial cell apoptosis. Resveratrol significantly decreased the mRNA levels of pro-inflammatory cytokines, namely Tumor Necrosis Factor-α (TNF-α), Interleukin-6 (IL-6) and Interleukin-22 (IL-22), while restoring the percentage of CD4+ cells to normal levels. It also attenuated oxidative stress by enhancing the activities of key antioxidant enzymes, including catalase (CAT), total superoxide dismutase (T-SOD), and glutathione peroxidase (GSH-Px). Furthermore, resveratrol reshaped the gut microbiota by increasing the abundance of obligate anaerobes Clostridium and decreasing the abundance of facultative anaerobes Enterobacteriaceae. The abundance of Lactobacillus and Dubosiella was also significantly increased by resveratrol. In summary, these findings demonstrate that resveratrol supplementation may serve as a promising nutritional strategy to ameliorate C. rodentium colitis by inhibiting CD4+ cell activation and remodeling the gut microbiota.
研究领域:
微生物组学
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免疫学
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炎症性肠病
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营养免疫
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分子营养学
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肠道屏障功能
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高影响因子
JOURNAL OF CONTROLLED RELEASE
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2025
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IF 11.5
Anning Yang,Hongwen Zhang,Huiping Zhang,Nan Li,Cong Chen,Xiaoling Yang,Jue Tian,Jianmin Sun,Guizhong Li,Yue Sun,Bin Liu,Yideng Jiang
Atherosclerosis (AS) therapy has been commonly based on lipid-lowering agents ( e.g. , statins), supplemented by other therapies, such as anti-inflammatory agents and antioxidants, through traditional Chinese herbs. Ferroptosis, a form of regulated cell death characterized by iron-dependent lipid peroxidation, has been implicated in the progression of AS, particularly in macrophages. In the study, we constructed a macrophage targeted hybridization nanodrug of HMLRPP NPs, which used Pit-loaded Poly(lactic- co -glycolic) acid (PLGA) nanoparticles and Res-loaded liposomes (Lipo) as nano-core, then, coated with a macrophage membrane hybridized by hyaluronic acid (HA). The nanodrug prolonged blood circulation time and achieved optimal Res and Pit accumulation in the atherosclerotic plaques by effectively evading immune system clearance. In vivo studies demonstrated that HMLRPP NPs significantly attenuated plaque progression, characterized by decreased plaque area, less lipid deposition, and increased collagen. Meanwhile, HMLRPP NPs inhibited macrophage ferroptosis by decreasing the expression of β-Hydroxybutyrate dehydrogenase 1 (BDH1), Orosomucoid 1 (ORM1) and enhancing the expression of Ribosomal protein S27-like (RPS27L), which resulted in the alleviation of lipid accumulation and inflammation. Our data suggest that the HMLRPP nanodrug delivery system with ferroptosis-regulating capability provides a feasible therapeutic strategy for atherosclerosis.
研究领域:
药理学
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心血管疾病
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纳米医学
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分子生物学
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PubMed
高影响因子
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
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2024
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IF 1.9
Liu Lilong,Wang Yexin,Dong Yanyan,Lin Shan,Guan Wenhui,Song Jia
The clinical application of 5-fluorouracil (5-Fu), a potent chemotherapeutic agent, is often hindered by its well-documented cardiotoxic effects. Nevertheless, natural polyphenolic compounds like resveratrol (RES), known for their dual anti-tumor and cardioprotective properties, are potential adjunct therapeutic agents. In this investigation, we examined the combined utilization of RES and 5-Fu for the inhibition of gastric cancer using both in vitro and in vivo models, as well as their combined impact on cardiac cytotoxicity. Our study revealed that the co-administration of RES and 5-Fu effectively suppressed MFC cell viability, migration, and invasion, while also reducing tumor weight and volume. Mechanistically, the combined treatment prompted p53-mediated apoptosis and autophagy, leading to a considerable anti-tumor effect. Notably, RES mitigated the heightened oxidative stress induced by 5-Fu in cardiomyocytes, suppressed p53 and Bax expression, and elevated Bcl-2 levels. This favorable influence enhanced primary cardiomyocyte viability, decreased apoptosis and autophagy, and mitigated 5-Fu-induced cardiotoxicity. In summary, our findings suggested that RES holds promise as an adjunct therapy to enhance the efficacy of gastric cancer treatment in combination with 5-Fu, while simultaneously mitigating cardiotoxicity.
研究领域:
肿瘤学
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心脏病学
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药理学
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分子生物学
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PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2025
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IF 12.4
Zihao Wei,Xiaomin Zhang,Tianqi Cao,Tian Luo,Yuanjing Sun,Zihan Jin,Changhu Xue
Quercetin (Que) and resveratrol (Res) exhibit significant synergistic effects in multiple health benefits. However, their sites of action in the gastrointestinal tract are not consistent. To achieve the targeted release of Que and Res into the small intestine and colon, respectively, core–shell–shell nanoparticles with programmed sequential release properties were constructed by layer-by-layer assembly technique in this work. Compared with hordein/pectin nanoparticles, hordein/pectin/carboxymethyl chitosan nanoparticles (HPCCS NPs) possessed higher physicochemical stability, encapsulation efficiency and loading capacity. Besides, the existence of CCS layers also significantly improved the co-delivery and programmed sequential release properties. To further optimize the performance of NPs, ultrasonic treatment was innovatively applied to Que/Res–HPCCS NPs. It was found that ultrasonic treatment enhanced the hydrogen bonding, hydrophobic interactions and electrostatic adsorption between molecules, thus improving the physicochemical stability, encapsulation efficiency and loading capacity of NPs. Furthermore, in vitro simulated gastrointestinal studies demonstrated that Que/Res–HPCCS NPs (ultrasound) had better programmed sequential release properties, which further improved the release rates of Res and Que in the small intestine and colon, respectively. This study is of great significance for the development of co-delivery systems with programmed sequential release of nutraceuticals, which can provide novel ideas for the development of related functional foods.
研究领域:
食品科学
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生物医学工程
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Chen-Chen Wang,Bao-Qiu Yang,Ran Feng,Han Tao,Bao-Cai Xu,Bao Zhang
In this study, biotin esterified debranched starch (Bio-DBS) nanoparticles with different molecular weights were prepared to improve the stability and antioxidant activity of resveratrol. The molecular weights of branched starch (DBS 3 , DBS 9 and DBS p ) determined by high-performance size-exclusion chromatography (HPSEC) were 3306, 3696, and 4688, respectively. Biotin was covalently coupled to DBS through the esterification reaction as a new material to prepare nanoparticles. The morphology, particle size, and loading capacity of Bio-DBS nanoparticles were all related to the molecular weights of DBS. The 1 H NMR results indicated that there was a hydrogen bonding interaction between Bio-DBS and resveratrol, which contributed to the photochemical and antioxidant activity of resveratrol in the nanoparticles. The highest encapsulation efficiency (78.9 %) and loading capacity (15.78 %) of resveratrol were observed in Bio-DBS 3 nanoparticles. Additionally, the cell viability was over 80 % when the concentration of Bio-DBS 3 reached to 200 μg/mL. The Bio-DBS nanoparticles significantly improved the thermal stability, photostability, and antioxidant properties of resveratrol. Therefore, the Bio-DBS nanoparticles prepared in this study can be used as a promising carrier to improve the stability and antioxidant activity of resveratrol and may have potential applications in oral delivery.
研究领域:
药学
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纳米技术
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食品科学
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PubMed
高影响因子
Evidence-based Complementary and Alternative Medicine
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2012
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IF 4.774
Yuan-yuan Chai,Fang Wang,Yan-li Li,Ke Liu,Hui Xu
Rheum emodi Wall has been reported to possess protective effect in many inflammatory diseases and oxidative stress-related injuries. This study aims to investigate antioxidant power of stilbenoids from R. emodi and then explore the material basis for its antioxidant potential. The most abundant stilbenoid piceatannol-4′-O-β-D-glucopyranoside (PICG) and its aglycon piceatannol (PICE) were isolated from R. emodi rhizome. Using well-accepted antioxidant chemicals as reference, antioxidant activity of these stilbenoids was examined by measuring DPPH and superoxide anion radical scavenging, ferric reducing power, and inhibition of lipid peroxidation in vitro. Both PICG and PICE displayed promising antioxidant activity in all the four assays. Comparisons among the tested compounds indicated that PICE has the most potent antioxidant activity and the presence of 3′-hydroxyl group may enhance antioxidant activity of stilbenoids. The antioxidative effect of PICE at the cellular level was further demonstrated on the model of hydrogen-peroxide-induced H9c2 rat cardiomyoblasts injury. Taking into account the rapid in vivo metabolic transformation of PICG into PICE it can be inferred that the most abundant stilbenoid PICG may be an important constituent responsible for the antioxidant potential of R. emodi and promising to be developed as an antioxidant agent for supplementary or therapeutic use.
研究领域:
药理学
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生物化学
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药物化学
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PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
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2019
·Vol. 35
·期 6
·1008-1011
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IF 1.071
Cao Yangyang,Cai Zengfei,Zhang Wenliang,Du Xiaohua
In this paper, a series of new β -methoxyacrylate derivatives containing a substituted phenylpyridine moiety was synthesized and bioassayed. The structures of all compounds were confirmed by nuclear magnetic re-sonance(NMR) and high-resolution mass spectrometry(HRMS). Preliminary bioassays demonstrated that compound 8 d showed more than 95% inhibition against Brassica juncea and Chenopodium serotinum at 37.5 g a.i./10 4 m 2 , when compared with the positive control halauxifen-methyl at 150 g a.i./10 4 m 2 . The present work demonstrated that compound 8 d exhibited the more promising herbicidal activity and may serve as a new herbicidal agent for herbicide.
研究领域:
植物学
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农药化学
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药理学
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高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2025
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IF 9
Qian Tan,Wentian Zhang,Cunxian Lai,Xinyu Zhi,Lichao Xia,Fangang Meng,Shanshan Zhao
Nanofiltration (NF) is a pivotal technology in advancing water purification, achieving a harmonious balance between high selectivity and energy efficiency. However, current thin-film composite (TFC) NF membranes often encounter challenges in simultaneously achieving high permeability and selectivity, while exhibiting robust antifouling performance. Herein, we introduce an innovative fabrication approach that incorporates resveratrol (Res) to form a functional interlayer to regulate interfacial polymerization (IP) process for developing a polyamide (PA) NF membrane with enhanced permeability and antifouling property. Ascribing to the hydrogen bonding and electrostatic interactions between the Res and piperazine (PIP), the aqueous phase diffusion rate of PIP molecules was effectively moderated, thus resulting in a finely controlled formation of an ultrathin (31.3 nm) and low cross-linked PA selective layer. The optimized Res-regulated TFC membrane exhibited a water permeance of 20.0 ± 1.0 L m -2 h -1 bar -1 , which is more than four times higher than that of control membrane. In addition, it presented exceptional ion selectivity for NaCl/ Na 2 SO 4 , with a value of 72.2, which is superior to the performance of most state-of-the-art PA NF membranes. Furthermore, it exhibited excellent antifouling properties during simulated and actual wastewater treatment. This environmentally friendly and cost-effective IP-regulation strategy shows great potential for design and engineering high-performance NF membranes for a wide range of water treatment applications.
研究领域:
水净化
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膜技术
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材料科学
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化学工程
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环境科学
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Binke Wang,Xinyu Qi,Ruping Yang,Xu Xu,Shiyao Li,Chunli Zhang,Haitao Yu
Liver cancer is one of the most prevalent and deadly malignancies worldwide, with limited effective therapeutic strategies. Although doxorubicin (DOX) is widely used as a frontline chemotherapeutic agent, its severe cardiotoxicity and lack of tumor selectivity significantly limited its clinical applications. Polydatin (PLD), a natural antioxidant and anticancer compound, has shown promise as an adjuvant for reducing DOX-induced cardiotoxicity, yet its poor bioavailability hinders its therapeutic potential. To improve the therapeutic efficacy of DOX while reducing systemic toxicity, we developed a hyaluronic acid (HA)-functionalized cubosome-based nanosystem co-loaded with DOX and PLD (1:3 ratio), i.e. HA 3% -D/P-CUBS + , to specifically target tumor sites with enhanced antitumor efficiency and alleviated DOX-induced cardiotoxicity. HA 3% -D/P-CUBS + significantly improved drug internalization and induced apoptosis in hepatocellular carcinoma cells (HepG-2 and HUH-7). Meanwhile, HA 3% -D/P-CUBS + protected normal hepatocytes (HL-7702 and AML-12) and reduced oxidative stress in cardiomyocytes (H9C2 cells). Notably, HA 3% -D/P-CUBS + inhibited in vivo tumor growth of 94 % without causing damage to vital organs, leading to improved survival rates. Moreover, it also mitigated DOX-induced oxidative stress by restoring the balance between oxidative and antioxidant systems. These findings highlight HA 3% -D/P-CUBS + as a promising and synergistic nanomedicine for targeted live cancer therapy, providing new insights into combination chemotherapy with enhanced safety and efficacy.
研究领域:
肿瘤学
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纳米医学
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药剂学
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分子药理学
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转化医学
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氧化应激
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Yanli Fu,Peipei Zhu,Hong Lin,Chun Hu,Lijuan Han,Weinong Zhang,Junbo He
The poor solubility and stability of resveratrol (Res) represent significant obstacles to its utilization and efficacy. Encapsulating in sodium caseinate (NaCas) has also faced issues concerning low encapsulation efficiency and stability. This study presented the synthesis of a novel NaCas and dialdehyde β-cyclodextrin (DA-β-CD) conjugate through the Schiff base reaction using a wet-heating method, which was then utilized to encapsulate resveratrol. Results showed the DA-β-CD was formed in the form of needle-like crystals, whereas the NaCas-DA-β-CD conjugate had a nanosheet structure. The NaCas-DA-β-CD exhibited a substantial increase in surface hydrophobicity, emulsifying activity index, and emulsion stability index. The incorporation of resveratrol into NaCas-DA-β-CD demonstrated a consistent and uniform dispersion at the nanoscale level. It achieved an encapsulation efficiency of 96.8% and exhibited excellent chemical stability, which surpasses those of the NaCas system. Furthermore, the water solubility and antioxidant capacity of resveratrol in NaCas-DA-β-CD exhibited characteristics similar to those in NaCas. The compacted nanosheet structure of the Res-NaCas-DA-β-CD complex, as observed in TEM and SEM, is likely responsible for those enhancements. Hence, the DA-β-CD can serve as an effective polysaccharide for protein modification, while the NaCas-DA-β-CD conjugate can function as an innovative delivery carrier for resveratrol and other hydrophobic bioactives.
研究领域:
食品科学
·
生物化学
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高影响因子
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