JOURNAL OF SEPARATION SCIENCE
·
2013
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IF 2.591
Jiangmei Li,Haiyun Zhai,Zuanguang Chen,Qing Zhou,Zhenping Liu,Zihao Su
A novel method combining molecular imprinting and SPE was developed in a capillary column for the determination of auramine O in shrimp. The capillary monolithic column was prepared by UV-initiated in situ polymerization, using auramine O as template and methacrylic acid and ethylene dimethacrylate as functional monomer and cross-linker, respectively. The properties of the prepared capillary monolithic column were investigated under the optimized conditions coupled with HPLC, and then the morphologies of the inner polymers were characterized by SEM. The calibration curve was expressed as A = 103 C + 19.8 ( r = 0.9992) with a linear range of 0.25–25.0 μg/mL, and the recoveries of auramine O at different concentrations in shrimp ranged from 90.5 to 92.4% with RSDs ranging from 2.1 to 4.4%. The capacities of the molecularly imprinted polymer and nonimprinted polymer columns were 0.722 and 0.147 μg/mg, respectively, and the LOD (S/N = 3) of auramine O in shrimp was 17.85 μg/kg. Under the selected conditions, the enrichment factors obtained were higher than 70-fold. The results indicate that the prepared molecularly imprinted capillary monolithic column was reliable and applicable to the analysis of auramine O in shrimp.
研究领域:
分析化学
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食品安全
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2021
·Vol. 45
·期 9
·e15754
·
IF 2.19
Jia-Liang Wang,Zhuo-Liang Yu,Fa-Wen Yin,De-Yang Li,Hui-Lin Liu,Liang Song,Da-Yong Zhou
The effects of different extraction solvents on the extraction yield and chemical composition of oils from by-products of marine shrimp Penaeus vannamei ( Pv ) and freshwater shrimp Procambarus clarkia ( Pc ) were investigated. Our results indicated that the ethanol and n-hexane mixture (4:1, v/v) was suitable solvent for simultaneous extraction of phospholipid and nonpolar lipids. By contrast, the ethanol and n-hexane mixture (4:1, v/v) and single ethanol were suitable solvents for the extraction of polyunsaturated fatty acids-rich (36.10%–42.78% of total fatty acids for Pv and 23.57%–25.58% of total fatty acids for Pc ) and phosphatidylcholine-rich (66.22–67.09 mol% of total phospholipids for Pv and 55.01–58.68 mol% of total phospholipids for Pc ) oils, but acetone was suitable solvent for the extraction of astaxanthin-rich oil (436.19 μg/g oil for Pv and 799.27 μg/g oil for Pc ). The findings will provide relevant information that can be used to improve the production of nutritional oils from shrimp by-products. Practical applications This study provides a theoretical basis for creating proper extraction processing to recover nutritional oils from shrimp by-products. For example, the ethanol and n-hexane mixture (4:1, v/v) was a suitable solvent for simultaneous extraction of phospholipid and non-polar lipids. By contrast, both of the ethanol and n-hexane mixture (4:1, v/v) and single ethanol were suitable solvents for the extraction of polyunsaturated fatty acids (PUFA)-rich and phosphatidylcholine-rich oils, but acetone was a suitable solvent for the extraction of astaxanthin-rich oil. Therefore, the oils extracted from shrimp by-products are rich in phospholipid, PUFA, and astaxanthin, which may be used as new functional food ingredients.
研究领域:
食品科学
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营养学
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海洋生物学
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产品关联
DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 23
·3768
·
IF 5.561
Ziwei Wang,Fujun Liu,Ying Luo,Xiangbo Zeng,Xuechen Pei,Guanhua Zhao,Min Zhang,Dayong Zhou,Fawen Yin
Antarctic krill (Euphausia superba) oil contains high levels of marine omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA), including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). In industrial production, krill oil is usually extracted from krill meals by using ethanol as a solvent. Water in the krill meal can be easily extracted by using ethanol as an extraction solvent. During the extraction process, the EPA and DHA are more easily oxidized and degraded when water exists in the ethanol extract of krill oil. Based on the analysis of peroxide value (POV), thiobarbituric acid-reactive substances (TBARS), fatty acid composition, and lipid class composition, the present study indicated that the composite antioxidants (TP-TPP) consist of tea polyphenol (TP) and tea polyphenol palmitate (TPP) had an excellent antioxidant effect. By contrast, adding TP-TPP into ethanol solvent during the extraction process is more effective than adding TP-TPP into krill oil after the extraction process.Keywords:antarctic krill;oil;antioxidant;tea polyphenol;composite antioxidants;ethanol
研究领域:
食品科学
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海洋生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2022
·
IF 8.91
Ming Wang,Jinlong Yan,Yumeng Xu,Xiangqian Zhou,Yusen Diao,Hui Wang,Jiahao Bian,Cheng Liu,Guixiang Quan
N-doping for the preparation of functional carbon materials is a trending research topic. In this study, N-rich biochar (BC) was prepared by calcining naturally N rich shrimp shells under oxygen-limiting environment, and the calcining temperatures were controlled. BC were activated with 5% hydrochloric acid solutions and then post-modified with ball-milling to obtain a series of novel adsorbents (MBCs). All samples were characterized by SEM, BET, FT-IR, XRD , XPS, TG, and element analysis. Surface area, pore volume, and other surface functional groups were significantly improved after acidizing and ball-milling. The adsorption capacities for MB were MBC350 > MBC500 > MBC650 >BC350 > BC650 > BC500, and the equilibrium adsorption capacities were 575.01 mg/g, 506.52 mg/g, 424.59 mg/g, 113.31 mg/g, 93.53 mg/g and 86.25 mg/g, respectively. The excellent adsorption performance of MBCs for MB was ascribed to Lewis acid-base interaction, π-π interaction, electrostatic interaction and van der Waals, and the quinone group and pyridinic-N on the surface of the MBCs are identified as the major active sites. Taken together, ball-milled shrimp shell biochar is a promising material for cation dye adsorption.
研究领域:
环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
AQUACULTURE
·
2022
·
IF 5.135
Xu Zhang,Li-Hua Hu,Da-Wei Song,Yang Hu,Jiong Chen
With the continuous expansion of cultural scale and the deterioration of cultural environment recently, various viral diseases occur frequently in cultured shrimp industry. White spot disease (WSD) caused by white spot syndrome virus (WSSV) results in a high mortality as well as a huge economic loss of shrimp culture. As a kind of Chinese herbal medicine extract, the application of cuminaldehyde in prevention and treatment of WSSV was investigated in this study. The data showed that cuminaldehyde at 40 μmol/L could significantly inhibit WSSV replication in shrimp within the highest inhibition rate of 89% at 72 hpi. The survival rate of WSSV-infected shrimp was increased by >50% after continuous treatment of cuminaldehyde versus the virus group at 144 hpi. Obviously, the horizontal transmission of WSSV was inhibited by cuminaldehyde in a static cohabitation challenge model, further weakening the spread of the virus to reduce the mortality of the virus-infected shrimp. Meanwhile, cuminaldehyde increased resistance of shrimp to WSSV by activating the innate immune response, which was essential for prevention of WSSV. Thus, cuminaldehyde could potentially be used as a therapeutic agent with a great potential in culture of shrimp seedling and may also be suitable for applications in pond aquaculture settings against WSSV transmission.
研究领域:
水产养殖学
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病毒学
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药理学
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产品关联
DOI
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2022
·
IF 4.622
Feng Hu,Yan Wang,Jingjie Hu,Zhenmin Bao,Mengqiang Wang
Lysozyme is relevant to the innate immune system as a vital protein for crustaceans. In the present study, we cloned and characterized a novel c-type lysozyme gene ( Lv LYZ) from the Pacific white shrimp ( Litopenaeus vannamei ). The obtained full-length cDNA of Lv LYZ was 990 bp and contained an open reading frame of 693 bp. Its deduced amino acid sequence consisted of 230 amino acids (aa) with a 17 aa signal peptide at the N-terminal and 130 aa functional domains. The multiple sequence alignment (MSA) indicated that the typical active sites in Lv LYZ were similarly conserved as c-type lysozymes from other species. The transcription of Lv LYZ appeared in all detected tissues and had relatively higher expression levels in hemocytes , hepatopancreas , gill and intestine. The mRNA expression profiles of Lv LYZ were up-regulated in hemocyte and hepatopancreas post the stimulation of Vibrio parahaemolyticus or white spot syndrome virus (WSSV), respectively. The recombinant protein of Lv LYZ (r Lv LYZ) exhibited antibacterial activities against various microbes, including Escherichia coli , Vibrio splendidus , Micrococcaus luteus , Vibrio parahaemolyticus and Staphylococcus aureus . These results indicated that Lv LYZ could cope with bacteria in L. vannamei and may play a significant role in immune response against invading pathogens.
研究领域:
分子生物学
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免疫学
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水产养殖学
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产品关联
DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2023
·
IF 3
Jiannan Chen,Xiuhua Xia,Peizhen Li,Hang Yu,Yunfei Xie,Yahui Guo,Weirong Yao,He Qian,Yuliang Cheng
As a type of environmentally deleterious compound, 4-nitrophenol (4-NP) has become a global concern due to its extreme toxicity to aquatic organisms and humans. Herein, we used naturally available crayfish shells as precursors to prepare a new type of carbon dots (CDs) by the hydrothermal method. The as-synthesized CDs exhibited strong blue fluorescence emission which can be sensitively quenched by 4-NP through the static quenching effect because of the formation of a ground state complex between CDs and 4-NP. Accordingly, a facile and rapid fluorescence sensor was developed to detect 4-NP from 0 μM to 50 μM with a limit of detection (LOD) of 0.16 μM. Furthermore, the proposed sensor was successfully applied for the detection of 4-NP in fresh crayfish meat and aquatic water samples.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
高影响因子
Journal of Future Foods
·
2025
·
IF 7.2
Xin Zhao,Shunliang Zhang,Xiaoyu Cheng,Bing Zhao,Shouwei Wang,Yan Zhao,Le Wang
Pretreatment methods and drying process play vital role in the quality and flavor changes of shrimp. The volatile and taste profiles in high pressure processing (HHP at 300MPa-5min, 300MPa-10min, 500MPa-5min, and 500MPa-10min) pretreatment shrimps after hot-air drying process were investigated by using the electronic tongue, electronic nose, high performance liquid chromatography, and gas chromatography/mass spectrometry. The results showed that HHP pretreatment endowed shrimps with better volatile and taste attributes to varying degrees, which significantly ( P < 0.05 ) increased total umami free amino acids (1.18−2.16 times), total sweet free amino acid (1.09−1.27 times) contents ( P < 0.05), promoted the production of pleasant taste nucleotides (1.52−1.83 times) and organic acids (1.19−1.78 times), and produced more volatile components (1.10−1.46 times) when compared to untreated group. Glycine, alanine, valine, lysine, histidine, arginine, guanosine monophosphate, inosine monophosphate, and succinic acid were main non-volatile active components. The equivalent umami values is: 500MPa-5min > 500MPa-10min > 300MPa-10min > 300MPa-5min > control group. Compared with control group, HHP pretreatment endowed shrimps with richer aldehydes, ketones, alcohols and pyrazine compounds, while control endowed shrimps with richer hydrocarbons. Based on these flavor profiles, the principal component analysis of E-tongue and E-nose data showed a distinct difference among the different pretreatment group. Generally, HHP at 500 MPa-5min was beneficial for improving EUC and volatile compounds of dried shrimps. The findings may provide theoretical reference for the development of high-quality drying technology for shrimps.
研究领域:
食品科学
·
食品加工
·
风味化学
·
水产制品
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分析化学
·
感官分析
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产品关联
DOI
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Yuean Chu,Mingyue Yu,Yunxing Li,Suoxian Yang,Hongyan Bi
Tracement of the origin of seafoods and aquatic products is an important issue for avoiding the falsification of production regions of aquatic products. Herein, by analyzing black tiger shrimp ( Penaeus monodon ) as a model of aquatic products, we demonstrated a method to trace the origin of aquatic products by analyzing the gut microbiome of black tiger shrimp ( Penaeus monodon ) collected from three commercial farms in China, via matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis. Multivariate analysis was executed on the obtained mass spectrometric datasets of bacteria extracted and collected from shrimp samples with different production regions. The gut microorganisms of black tiger shrimps can be identified by scoring the gut microbiota of black tiger shrimps collected from three producing areas. The black tiger shrimp from three production regions were found with distinguished characteristics of bacterial prevalence in their digestive gland microbiota. The dominant bacteria genus in digestive gland microbiota from black tiger shrimp samples from the three production regions are completely different. Single-blind shrimp samples with unknown production region was successfully screened by comparing the detected MS data in different ways. This strategy can trace the production region of the analyzed shrimp samples in a quick and efficient mode. The application of the present protocol was further demonstrated by analyzing the biometric species from gill tissue of Chinese mitten crab ( Eriocheir sinensis ) samples collected from crab-producing areas in Jiangsu Province (China), that is, Taihu Lake, Gaoyou Lake, and Hongze Lake for tracing their production area. It is expected to extend the presently developed method to provide clues for the origin traceability of other aquatic products.
研究领域:
水产养殖
·
食品科学
·
微生物学
·
质谱分析
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产品关联
DOI
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
·
2018
·
IF 1.684
Shiwei Xie,Dan Wei,Peng Yin,Lu Zheng,Tianyu Guo,Yongjian Liu,Lixia Tian,Jin Niu
An 8-week feeding trial was conducted to evaluate the effect of fish-meal replacement on growth performance, protein synthesis and immune response of juvenile Pacific white shrimp, Litopenaeus vannamei reared at low salinity (7‰). Five isonitrogenous and isolipidic diets were formulated to contain graded levels (25, 20, 15, 10 and 5%) of fish-meal. High quality alternative solutions were performed, crystalline amino acids, phytase, mannan oligosaccharides and some micro-nutrients were supplemented in the low fish-meal diets. Each diet was randomly assigned to triplicate tanks, each tank with 30 shrimp (mean weight 0.3 g), the shrimp were fed 3 times a day. Weight gain and survival were decreased with the decreasing dietary fish meal levels. When dietary fish-meal decreased, the gene expression of TOR, Raptor and eIF4E2 in hepatopancreas were decreased with the decreasing fish meal levels, eIF4E2 in intestine was decreased while 4E-BP was increased with the decreasing fish meal levels. The mRNA level of SOD in hepatopancreas decreased, and the expression of GPx and CAT increased with the decreasing FM levels. The Toll pathway was affected by dietary FM levels, the expression of Toll2, TNFSF, MyD88, Rho and p38 in intestine were increased with the decreasing FM levels. The results indicated that at low salinity condition, fish meal level lower than 15% would inhibit the protein synthesis and harm to the health of shrimp.
研究领域:
水产养殖
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分子生物学
·
营养学
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DOI
PubMed
高影响因子
AQUACULTURE INTERNATIONAL
·
2024
·Vol. 32
·期 5
·5461-5494
·
IF 2.2
Alhoshy Mayada,Shehata Akram Ismael,Habib Yusuf Jibril,Wang Xuexi,Wang Yilei,Zhang Ziping
Crayfish species like Cherax quadricarinatus are crucial nutrients that contribute to the world economy but face challenges in production due to dietary supplements. Octacosanol offers diverse health benefits, from immunity boosts to anti-inflammatory and anti-tumor effects. The current investigation assessed the ovarian changes, immune reaction, availability of antioxidants, gene response pattern, and entire organ performance of the red claw crayfish after supplementation with different levels of octacosanol. Two hundred twenty-five C. quadricarinatus with an initial weight of 53 ± 1.11 (g/crayfish) were randomly stocked into fiberglass tanks (15 crayfish/tank) and fed each day with three diverse dietary octacosanols. Different levels of octacosanol (4, 8, 12, and 16 mg/kg) were tested in triplicates over 60 days, denoted as D1, D2, D3, and D4, respectively, excluding the control group. The study revealed that 8 mg/kg octacosanol effectively enhances growth and feed utilization in C. quadricarinatus , while also improving ovarian performance by increasing hepatosomatic index (HSI), gonadosomatic index (GSI), and condition factor (CF). Additionally, it decreased levels of MDA and NO in crayfish. Supplementation with 16 mg/kg octacosanol boosted antioxidant capacity by enhancing activities of T-AOC and SOD, as well as influencing nonspecific immune enzymes in the hepatopancreas at doses of D3 (12 mg/kg) and D4 (16 mg/kg). Furthermore, it positively impacted GLU and protein profiles while reducing levels of T-BIL, T-CHOL, TG, LDL-c, and VLDL-c in crayfish. These dietary interventions also regulated gene expression associated with reproduction, lipid metabolism, antioxidants, and immune response in various organs of C. quadricarinatus . Octacosanol demonstrated potential as a safe and effective feed additive. According to quadratic regression analysis, nutritional supplementation of octacosanol at 9 mg/kg diet is considered the recommended dosage in the red claw crayfish diet.
研究领域:
水产养殖学
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营养学
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分子生物学
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DOI
高影响因子
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
·
2020
·Vol. 122
·期 4
·1900325
·
IF 2.056
Li Pan,Xin Zhang,Xiaozu Fan,Hua Li,Baocheng Xu,Xueqin Li
In this work, whey protein isolate (WPI)-coated astaxanthin-loaded liposomes are prepared by combining the fabrication of liposomes with the layer self-assembly deposition technique. The physical properties of such composite carriers are evaluated by zeta potential, particle size, distribution, encapsulation efficiency, and morphology. WPI- coated astaxanthin-loaded liposomes display homodisperse distribution and high encapsulation efficiency with a WPI coating layer surrounding the surface of conventional liposomes by the electrostatic interaction. Fourier transform infrared spectroscopy and X-ray diffraction analysis reveal that WPI interacts with the lipid bilayer via hydrophobic forces and hydrogen bonding, which result in the successful coating. Based on experimental results of differential scanning calorimetry and thermogravimetric analysis, it is demonstrated that thermal stability of astaxanthin-loaded liposomes benefits from the formation of surface modification of the WPI-layer. In addition, the physical stability of WPI-coated astaxanthin-loaded liposomes under heating and light is significantly improved as compared with uncoated liposomes. This research might provide scientific guidance for the development of WPI-coated liposomes as efficient carrier systems for bioactive substances in food and pharmaceutical industry. Practical Applications : To improve lipid membrane stability and to prevent the leakage of encapsulated astaxanthin, a novel carrier system based on WPI coated on the surface of liposomes is prepared through the layer self-assembly deposition technique. This research suggests that WPI-coated liposomes represent an effective and stable delivery system for astaxanthin. WPI-coated liposomes could be developed as efficient carrier systems for bioactive compounds in the food and pharmaceutical industries.
研究领域:
食品科学
·
药物传递系统
·
生物材料
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）