Biosensors-Basel
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2024
·Vol. 15
·期 1
·5
·
IF 4.9
Jianxiao Fang,Yunting Jia,Zelong Liao,Bairui Qi,Tao Huang
The early monitoring of cardiovascular biomarkers is essential for the prevention and management of some cardiovascular diseases. Here, we present a novel, compact, and highly integrated skin electrode as a mechanical–electrochemical dual-model E-skin, designed for the real-time monitoring of heart rate and sweat ion concentration, two critical parameters for assessing cardiovascular health. As a pressure sensor, this E-skin is suitable for accurate heart rate monitoring, as it exhibits high sensitivity (25.2 pF·kPa−1), a low detection limit of 6 Pa, and a rapid response time of ~20 ms, which is attributed to the iontronic sensing interface between the skin and the electrode. Additionally, the electrode functions as a potassium ion-selective electrode based on chemical doping, achieving an enhanced response of 11 mV·mM−1. A test based on the real-time monitoring of a subject riding an indoor bike demonstrated the device’s capability to monitor heart rate and sweat potassium ion levels reliably and accurately. This advancement in wearable technology offers significant potential for enhancing patient care based on the early detection and proactive management of cardiovascular conditions.
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Reaction Chemistry & Engineering
·
2024
·
IF 3.4
Zhiwei Huang,Yaxin Yuan,Xinyi Li,Yiyang Li,Min Wang,Zhuqing Wang
E-waste contains a variety of non-renewable precious metal resources, and the amount is significantly higher than the abundance of precious metals in the corresponding ores. Therefore, it is of great significance to recover and reuse precious metals in e-waste. In this study, we successively used a simple one-step oxidation method and physical cross-linking to prepare a poly-m-phenylenediamine composite membrane material (CMC–PmPD composite membrane) containing a large number of recycling groups. The prepared CMC–PmPD composite membrane has high adsorption capacity and adsorption efficiency for Au(III), and the maximum adsorption capacity for Au(III) reaches 421.1 mg g−1. The adsorption follows a second-order kinetic process and a Langmuir isotherm model, indicating that the adsorption mechanism is a monolayer chemisorption. The regeneration of the composite membrane material can be realized after a simple thiourea solution immersion, and 89.5% adsorption efficiency is maintained after five regeneration cycles. In addition, when the CMC–PmPD composite membrane was applied to the treatment of mixed heavy metal ion solutions with different concentrations and compositions, the CMC–PmPD composite membrane was always able to selectively adsorb more than 95% of Au(III) from the simulated solution or e-waste leachate, which was highly selective and applicable. The CMC–PmPD composite membrane has a broad application prospect in metallurgy and fine chemical industry.
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JOURNAL OF COORDINATION CHEMISTRY
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2025
·
IF 2.1
Mingzhe Li,Yuyang Zhou,Hua Zhou,Dingqiang Lu
Vitamin E is an important fat soluble antioxidant, its acetate is the main derivative product of vitamin E. Using metal organic framework material ZIF-8 as a catalyst, vitamin E acetate (VEA) was prepared by catalyzing the esterification reaction of D-α-tocopherol and acetic anhydride in a solvent-free system. Firstly, the preparation conditions of metal organic framework materials were studied: different imidazole ligands, different zinc ion ligands, molar concentration ratio of Zn2+ to 2-methylimidazole(2-mIM), and preparation time. The results showed that the catalytic performance of ZIF-8 was optimal when Zn(CH3COO)2·2H2O was used as the zinc source, 2-mIM was used as the imidazole ligand, the molar concentration ratio of Zn2+ / 2-mIM was 1:20, and the preparation time was 1 hour. The yield of vitamin E acetate was 86.85%. The synthesis process of VEA was optimized by single factor experiments such as substrate molar ratio, reaction time, dosage of nano enzyme, reaction temperature and so on. The results showed that the yield of vitamin E acetate reached 96.31% under the optimal conditions of n (D-α-tocopherol) =1mmol, [n (D-α-tocopherol) : n (Acetic anhydride)] =1:15, reaction time 24 h, ZIF-8 dosage 0.05 g, reaction temperature 50 °C.
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Advanced Science
·
2024
·
IF 14.3
Zhao Huang,Li Zhou,Jiufei Duan,Siyuan Qin,Jingwen Jiang,Haining Chen,Kui Wang,Rui Liu,Minlan Yuan,Xiangdong Tang,Edouard C. Nice,Yuquan Wei,Wei Zhang,Canhua Huang
Loss of E-cadherin (ECAD) is required in tumor metastasis. Protein degradation of ECAD in response to oxidative stress is found in metastasis of hepatocellular carcinoma (HCC) and is independent of transcriptional repression as usually known. Mechanistically, protein kinase A (PKA) senses oxidative stress by redox modification in its β catalytic subunit (PRKACB) at Cys200 and Cys344. The activation of PKA kinase activity subsequently induces RNF25 phosphorylation at Ser450 to initiate RNF25-catalyzed degradation of ECAD. Functionally, RNF25 repression induces ECAD protein expression and inhibits HCC metastasis in vitro and in vivo. Altogether, these results indicate that RNF25 is a critical regulator of ECAD protein turnover, and PKA is a necessary redox sensor to enable this process. This study provides some mechanistic insight into how oxidative stress-induced ECAD degradation promotes tumor metastasis of HCC.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Bo Zhang,Tong Wang,Siyu Ni,Yaping Zong,Nan Wang,Yunlong Si,Yong Li
Recovery of bioactive proteins from insoluble inclusion bodies (IB) is a critical challenge when purifying recombinant proteins expressed in E. coli . One practical solution is to enhance the renaturability or refolding capability of the target protein after the insoluble proteins are solubilized with denaturants such as urea. While some methods, such as using chemical additives and manipulating the physical environment, have been reported to assist in protein refolding, their effectiveness remains limited. This work evaluated the potential of a highly conserved domain found in five biotin-dependent carboxylases as a fusion tag for purifying IB proteins. We found that this tag, comprised of 67 amino acids (named P67) as eight anti-parallel β-sheets that collectively form a flattened barrel structure, significantly improved the refolding process of its IB protein partner. The yield of bioactive proteins fused with this tag recovered from the dialysis stage was markedly higher than untagged ones. We further proved that this effect relies on the structural integrity of the tag's spatial conformations. Under natural conditions, soluble proteins can be purified using the P67 tag via the biotin/streptavidin affinity system. In conclusion, this tag offers a promising methodology for purifying both insoluble and soluble recombinant proteins.
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Journal of Materials Chemistry B
·
2025
·
IF 5.7
Yingchong Chen,Wenhui Hu,Lizhen Huang,Yuling Liu,Fang Li,Haibin Zou,Pengfei Yue
In glioma treatment, the poor penetration of therapeutic drugs through the blood–brain barrier (BBB) to the tumor region, along with the intrinsic resistance of glioma cells via multiple survival mechanisms, remain major challenges. Liposomes are a popular choice for glioma therapy due to their ability to help drugs penetrate the BBB. However, unmodified liposomes have drawbacks, such as poor stability, low drug entrapment efficacy, and rapid removal from circulation, leading to reduced drug absorption at the lesion sites. To address these issues, a novel F127-grafted vitamin E succinate (F127–VES) was designed to prepare multifunctional modified liposomes for delivery of harmine (HAR). Compared to conventional liposomes (Ordinary-Lip), F127–VES-Lip shows greater potential in enhancing the anti-glioma effect of HAR. This is attributed to several key features: Firstly, Pluronic can enhance the transport across the blood–brain barrier. Secondly, the presence of F127 in the formulation extends the circulation time of the drug in the bloodstream. Finally, the combination of F127 and VES in F127–VES-Lip can induce tumor cell apoptosis. In vitro and in vivo results confirmed that the F127–VES-Lip could significantly elevate cellular uptake, promote apoptosis and increase the antitumor effect for C6 glioma cells. The F127–VES-Lip is a promising nanoformulation to enhance the effect of HAR for glioma treatment.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Xiaotian Xu,Shi-Fan Xue,Jiale Wang,Wei Zhang,Da Wu,Guoyue Shi,Min Zhang
Selective detection of phenolic compounds is imperative for environmental surveillance and public health safeguards. This study introduces a dual-electrode voltametric electronic tongue (e-tongue) leveraging transition metal oxide-nanoengineered laser-induced graphene (LIG) to resolve phenol and its cresol isomers (o-, m-, p-) despite overlapping electrochemical signals. Contrary to conventional single-electrode systems hindered by matrix-induced peak overlaps, our e-tongue employs a synergistic sensing arraying: MnO 2 /LIG and Bi 2 O 3 /LIG. These composites are fabricated via a one-step in situ laser doping process, ensuring uniform nanoparticle integration while mitigating distributional inhomogeneity and interfacial weaknesses associated with post-synthetic modifications. Both MnO 2 /LIG and Bi 2 O 3 /LIG exhibit augmented electroactive surface areas, conductivity, electron transport kinetics, hydrophilicity, and stability relative to pristine LIG. The dual-electrode system achieved robust differentiation of phenolics in buffered solutions, environmental water samples, and tobacco smoke matrices. This work pioneers a scalable nanoengineering strategy for multi-target analysis of structurally proximal pollutants, advancing point-of-need environmental monitoring technologies.
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Advanced Composites and Hybrid Materials
·
2024
·Vol. 7
·期 5
·1-11
·
IF 23.2
Wen Xiaohong,Zhao Zengcai,Chen Yuchang,Shan Xinzhi,Zhao Xuefeng,Gao Xiumin,Zhuang Songlin
Multifunctional integrated flexible electronic skin (e-skin) is the essential medium for information exchange between humans and machines. Especially, the proximity/pressure/strain sensing has become a technological goal for various emerging wearable electronic devices, such as biomonitoring devices, smart electronics, augmented reality, and prosthetics. Herein, a stretchable hybrid electronic network-based e-skin is presented, fabricated by embedding 3D hollow MXene spheres/Ag NWs hybrid nanocomposite into PDMS, which can effectively avoid the electrode falling off due to stress concentration. This e-skin works in noncontact mode (proximity-negative capacitance) and contact mode (pressure-positive capacitance and strain-resistance) for multiplex detection of random external force stimuli without mutual interference. The macroscopic physical structure of stretchable electrodes and the microscopic hybrid three-dimensional conductive network jointly contribute to the good sensing performance of the device. This work provides an effective and universal strategy for the application of wearable intelligent electronic products that demand noncontact interaction and multimodal tactile perception.
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Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Tong Feifei,Qin Zongmin,Wang Hongyue,Jiang Yingying,Li Junkuan,Ming Hui,Qu Ge,Xiao Yazhong,Sun Zhoutong
Chiral amino alcohols are prevalent synthons in pharmaceuticals and synthetic bioactive compounds. The efficient synthesis of chiral amino alcohols using ammonia as the sole amino donor under mild conditions is highly desired and challenging in organic chemistry and biotechnology. Our previous work explored a panel of engineered amine dehydrogenases (AmDHs) derived from amino acid dehydrogenase (AADH), enabling the one-step synthesis of chiral amino alcohols via the asymmetric reductive amination of α-hydroxy ketones. Although the AmDH-directed asymmetric reduction is in a high stereoselective manner, the activity is yet fully excavated. Herein, an engineered AmDH derived from a leucine dehydrogenase from Sporosarcina psychrophila (SpAmDH) was recruited as the starting enzyme, and the combinatorial active-site saturation test/iterative saturation mutagenesis (CAST/ISM) strategy was applied to improve the activity. After three rounds of mutagenesis in an iterative fashion, the best variant wh84 was obtained and proved to be effective in the asymmetric reductive amination of 1-hydroxy-2-butanone with 4-fold improvements in kcat/Km and total turnover number (TTN) values compared to those of the starting enzyme, while maintaining high enantioselectivity (ee >99%) and thermostability (T5015 >53°C). In preparative-scale reaction, the conversion of 100 and 200 mM 1-hydroxy-2-butanone catalyzed by wh84 was up to 91–99%. Insights into the source of an enhanced activity were gained by the computational analysis. Our work expands the catalytic repertoire and toolbox of AmDHs.
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PROCESS BIOCHEMISTRY
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2010
·
IF 2.444
Runrong Ding,Zhaofeng Li,Sheng Chen,Dan Wu,Jing Wu,Jian Chen
The purpose of this study was to investigate the effect of medium additives on the secretion of recombinant α-cyclodextrin glucosyltransferase (α-CGTase) into the culture media of Escherichia coli . It is found that supplementation of the E. coli culture with SDS, glycine, Ca 2+ or Na + , individually, facilitated the secretion of α-CGTase. Orthogonal experiment showed that the optimal condition to achieve maximal secretion of α-CGTase was the supplementation with 0.03% SDS, 400 mM Na + , 0.3% glycine and 10 mM Ca 2+ together. Under this condition, extracellular enzyme activity reached 12.89 U/ml, which is 15 times higher than that of the culture without any additives. Further analysis showed that the permeability, fluidity and phosphatidylglycerol content of the E. coli cell membrane under the optimized condition were significantly increased in comparison to those under the control condition. These might be the potential mechanisms for the increased secretion of α-CGTase from the periplasmic compartment into the culture medium.
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iScience
·
2023
·
IF 5.8
Yao Li,Zi-Yu Liang,Hai-Lin Wang
Summary Red blood cells supply the oxygen required for all human cells and are in demand for emerging blood-loss therapy. Here we identified N6-methyl-2′-deoxyadenosine (6mdA) as an agonist that promotes the hyperproliferation of burst-forming unit erythroid (BFU-E) progenitor cells. In addition, 6mdA represses the apoptosis of erythroid progenitor cells (EPCs). Combined use of with SCF and EPO enabled cultures of isolated BFU-E to be expanded up to 5,000-fold. Transcriptome analysis showed that 6mdA upregulates the expression of the EPC-associated factors c-Kit, Myb, and Gata2 and downregulates that of the erythroid maturation-related transcription factors Gata1, Spi1, and Klf1. Mechanistic studies suggested that 6mdA enhances and prolongs the activation of erythropoiesis-associated master gene c-Kit and its downstream signaling, leading to expansion and accumulation of EPCs. Collectively, we demonstrate that 6mdA can efficiently stimulate the EPC hyperproliferation and provide a new regenerative medicine recipe to improve ex vivo generation of red blood cells.
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Nano Research
·
2020
·Vol. 14
·期 4
·1043-1052
·
IF 8.183
Niu Ben,Yang Su,Hua Tao,Tian Xiao,Koo MingKin
Electronic textiles (e-textiles), known as a newly-developed innovation combining the textile and electronic technologies, are burgeoning as the next-generation of wearable electronics for lots of promising applications. However, a big concern is the durability of the e-textiles during practical using. Here, we describe a facile method to fabricate mechanically and electrically durable e-textiles by chemical deposition of silver nanoparticles (AgNPs) on widely used cotton fabric. The interface between AgNPs and fabric was tightly strengthened by the bioinspired polydopamine, and a highly waterproof and anticorrosive surface was further obtained by modifying with a fluorine containing agent of 1 H ,1 H ,2 H ,2 H -perfuorodecanethiol (PFDT). In addition to the low sheet resistance of 0.26 ohm/sq and high conductivity of 233.4 S/cm, the e-textiles present outstanding stability to different mechanical deformations including ultrasonication, bending and machine washing. Moreover, thanks to the surface roughness of AgNPs and low surface energy of PFDT, a superhydrophobic surface, with a water contact angle of ca. 152°, was further obtained, endowing the e-textiles excellent anti-corrosion to water, acid/alkaline solution and various liquids (e.g., milk, coffee and tea). Finally, the application of this highly conductive e-textiles in wearable thermal therapy is demonstrated. Together with the facile, all-solution-based, and environmentally friendly fabrication protocol, the e-textiles show great potential of large-scale applications in wearable electronics.
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