CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Mei Xiang,Dongfang Wu
A detailed investigation was carried out to develop novel pore- and morphology-controllable ETS-10 zeolite. The XRD , N 2 adsorption/desorption, SEM, TEM and 29 Si NMR MAS results proved that a series of hierarchical ETS-10 zeolites was successfully synthesized and more importantly, their morphologies and pore sizes can be freely confined in the desired range under the mesoporogen, morphology- and structure-directing actions of different additives. What's more, the close relationship between various additives and raw materials especially titanium source was discussed, which played an active part in morphology change and hierarchical pores formation. This can be ascribed to the special reducibility of inorganic Ti source, system liquidity and pH, and the enhanced inorganic species dissolution. Finally, the synthesized hierarchical ETS-10 materials were used as heterogeneous catalysts for different biomass-derivatives hydrogenation. Remarkably catalytic performance can be attributed to the unique TiO 6 octahedra carrying two negative charges that strengthens the interaction with active metal species, and thus improves their dispersion. Moreover, the inherent strong Lewis basicity and moderate Lewis acidity favor the oriented conversion of reactants. The controllably hierarchical structure and morphology benefit exposure of more highly dispersed active sites and prolong the catalyst life circle. Especially, an analogous molecular-recognition pattern was found by using sodium lignosulphonate, which is identified as the leading role in noticeably enhancing the catalytic activity and selectivity of the correspondingly ETS-10-based catalyst.
研究领域:
材料科学
·
催化
·
化学工程
·
无机化学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Materials Technologies
·
2023
·Vol. 8
·期 17
·2300367
·
IF 6.8
Yongxing Lin,Pengfei Qi,Jincheng Liu,Yizhi Zhuo,Cheng Cai,Haibao Zhang,Xiangyang Li,Xianglan Liu,Lin Chen,Sihai Luo,Xingyou Tian
Large-area gold-coated polyamide 66 (PA66) nanopillar (PA66NP@Au) arrays consisting of secondary lamellar structures and sub-10–nm gaps are fabricated for surface-enhanced Raman spectroscopy (SERS) using ultrathin anodized aluminum oxide (AAO) template, solution-phase growth of PA66 lamellae, and Au sputtering. Such closely packed PA66NP@Au arrays with high density of electromagnetic field enhancement (“hot spots”) on large area exhibit high SERS sensitivity for detection of rhodamine 6g (R6G) molecules with concentration down to 10 −14 m and a large Raman analytical enhancement factor (AEF) of ≈10 10 . Furthermore, the as-fabricated PA66NP@Au arrays provide highly sensitive Raman signals in detection of a commonly pesticide residue, carbendazim with a limit of detection (LOD) of 0.05 mg L −1 (0.05 ppm), which is lower than that of maximum residue limit of 0.2 ppm allowed in Europe Union. The as-fabricated PA66NP@Au arrays are promising for highly sensitive and uniform SERS detection.
研究领域:
材料科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 20
·12185-12193
·
IF 3.108
Yuhua Zhang,Keming Jiang,Degang Qing,Bing Huang,Jiayi Jiang,Shumei Wang,Chunyan Yan
Camptotheca acuminata Decne (Nyssaceae) is a major natural source of the anticancer drug camptothecin (CPT) and its derivatives. The leaves and stems are two of the main regions that accumulate CPT in C. acuminata. In this study, we successfully isolated cambial meristematic cells (CMCs) and dedifferentiated cells (DDCs) from C. acuminata stems and leaves, identified their characteristic features using micrograph analysis, and assessed their hypersensitivity to γ-irradiation and zeocin. The growth and kinetics curves of CMCs and DDCs were also studied. Furthermore, the accumulation of CPT and 10-hydroxycamptothecin (HCPT)—a more potent and less toxic CPT derivative—was assessed by liquid chromatography, and the transcriptional levels of nine genes encoding key enzymes involved in CPT and HCPT biosynthesis was assessed using quantitative real-time polymerase chain reaction (PCR) in CMCs and DDCs. The results showed that CMCs induced were particularly hypersensitive to γ-irradiation and zeocin and presented with abundant and small vacuoles, whereas DDCs were not. The above morphological and physiological characteristics of CMCs were consistent with previous reports. B5 medium was determined as the best growth medium for CMCs and DDCs. The accumulation of CPT and HCPT was higher in CMCs than DDCs, and the expression of IPI, G10H, ASA1, TSB, TDC1, TDC2, and STR was significantly upregulated in CMCs compared with DDCs; HMGR2 and HMGR3 were downregulated in CMCs. We speculate that the high accumulation of CPT and HCPT in CMCs was due to the upregulation of these seven genes.
研究领域:
药理学
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植物生物学
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2024
·Vol. 68
·期 19
·2400098
·
IF 4.5
Xinqi Huang,Lu Xiu,Yumei An,Yuan Gong,Sunao Li,Xueshi Chen,Chao Liu,Jianghuiwen Lu,Haiyan Shan,Jie Chang,Mingyang Zhang
The objective of this study is to elucidate how Royal jelly (RJ) and 10-hydroxy-2-decanoic acid (10-HDA) prevents diabetic skin dysfunction by modulating the pyroptosis pathway. Type 2 diabetes models are induced by fat diet consumption and low dose of streptozotocin (STZ) in C57BL/6J mice and treated with RJ (100 mg kg –1 day –1 ) and 10-HDA, the major lipid component of royal jelly (100 mg kg −1 day −1 ) for 28 weeks. The results show that serum concentrations of glucose and triglyceride are significantly lower in the RJ group or 10-HDA than diabetes mellitus (DM) group. Compared to the control group, pyroptosis proteins, GSDMD, ASC, Caspase-1, and IL-1β are increased in the skin of the diabetic model, accompanied by the activation of the Wnt/β-catenin signal pathway. Further evaluations by RJ exhibit superior improvement of skin damage, repress activation of the Wnt/β-catenin pathway, and attenuate keratinocyte pyroptosis, but 10-HDA cannot completely suppress the activation of Wnt/β-catenin pathway and pyroptosis, which shows a relatively weak protective effect on skin damage which shows that RJ is a better effect on skin injury after DM.
研究领域:
药理学
·
内分泌学
·
皮肤病学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Qihong Zhang,Chen Su,Zhaohui Lu,Hui Wang,Zongmiao Feng,Alexandr V. Dushkin,Weike Su
An amphiphilic conjugate of carboxymethyl xylan-nonanoic acid (CX-NA) was synthesized with molecular weight of 38.35 kDa, HLB value of 13.59, and critical micelle concentration of 23.17 μg/ml. CX-NA could efficiently encapsulate the model drug of 10-hydroxycamptothecin (HCPT). The drug loaded amphiphilic conjugate could self-assembled to micelles with an average diameter of 110 nm, zeta potential of −42.88 mV, and drug encapsulation efficiency of 79.8%. In vitro experiments confirmed that the drug-loaded micelles exhibited excellent stability and permeability in the intestinal environment. Transport pathway demonstrated that HCPT was uptake by cells through clathrin-mediated endocytosis. Intestinal in situ absorption study further confirmed CX-NA vehicle could enhance HPCT to transport across intestinal epithelial cells in colonic tissues. Furthermore, the formulation showed excellent anti-tumor activity in vitro and improved bioavailability of 3.4 times in vivo as comparing with free HCPT. These findings imply that this amphiphilic conjugate is a potential and promising vehicle for delivery anticancer drug.
研究领域:
药学
·
药物递送系统
·
药理学
·
纳米技术
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生物药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2021
·
IF 5.875
Wei He,Yawei Du,Tao Wang,Ji Wang,Lei Cheng,Xinsong Li
7-Ethyl-10-hydroxycamptothecin (SN38), a potent camptothecin derivative specifically targeting DNA topoisomerase I cleavage complexes, has shown great potential in the treatment of solid tumors . Because of its poor solubility and chemical and metabolic stability, the clinical application of SN38 is highly limited. To address these problems, a novel redox-responsive SN38 conjugate based liposomal formulation is developed in this report. First, SN38 was conjugated with lysophospholipid by using a cleavable disulfide bond linker. After that, the conjugate (SN38-SS-PC) was assembled into liposomes by thin film method. Dynamic light scattering (DLS) characterization indicated that SN38-SS-PC liposomes possessed a narrow size distribution (172.8 ± 10.5 nm) and negative charged zeta potential (-8.9 ± 0.3 mV). The results of storage and physiological stabilities showed that SN38-SS-PC liposomes was stable under different conditions. More importantly, a reduction responsive release of parent drug SN38 was observed in the medium containing glutathione (GSH). In addition, SN38-SS-PC liposomes had a much more rapid cellular uptake behavior against cancer cells. The enhanced anti-cancer efficacy of SN38-SS-PC liposomes was further demonstrated by in vitro cytotoxicity assay against MCF-7 and A549 cells. Under in vivo evaluation in 4 T1 xenograft tumor model, SN38-SS-PC liposomes were observed to have lower systemic toxicity and higher tumor inhibition rate of 53.3% compared with the commercialized SN38 prodrug Irinotecan (Ir). In summary, SN38-SS-PC liposomes could be a promising redox responsive delivery system of SN38 for cancer therapy.
研究领域:
药学
·
肿瘤学
·
药物递送系统
·
生物药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2016
·
IF 2.326
Qin Tang,Fangling Ji,Jianli Guo,Jingyun Wang,Yachen Li,Yongming Bao
Chrysin , one of natural flavonoid compounds, has recently been found to possess anti-inflammatory, antiallergic and anticancer properties. To increase its anticancer effects, 5 chrysin derivates were synthesized on the base of DNA intercalator structure. The inhibiting effects of chrysin and its derivatives on cancer cells Hela, BGC823, MCF-7, HepG2, and normal cells HEK-293, were evaluated by MTT assays . 5-(2′-amino) phenyl-7-cyclohexanemethylchrysin (Ch-1), a unique chrysin derivate, killed all the cancer cells but kept above 60% survival rate in normal cells HEK-293 at 62.5 μM. Treated with chrysin from 250 μM to 500 μM, those cells were still maintained above 60% survival rate. The result of circular dichroism spectra showed that Ch-1 could intercalate DNA while chrysin had no effects on DNA. Interestingly, Hela cells survival rates were 95% and 10%, after treated with 20 μM and 30 μM of Ch-1, respectively. Both intrinsic and extrinsic apoptotic pathway were identified in regulating the cell death caused by Ch-1 in Hela cells. p53, the upstream regulator of apoptotic pathway were extremely significantly up-regulated in Hela cells treated with 25 μM Ch-1. Moreover, the inhibiting effects and apoptotic related proteins responses to Ch-1 on Hela cells were abolished after pre-treated with Pifithrin-α (Pft-α), a p53 inhibitor. So, p53-depedent apoptosis is the crucial factor governing the inhibiting effects of Ch-1 in Hela cells. Amazingly, Ch-1 at non-toxic concentration (2.5–10 μM) enhanced significantly anti-cancer effect of 10-hydroxy camptothecin (HCPT) on Hela, BGC823, and MCF-7 cells.
研究领域:
癌症研究
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药理学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
GREEN CHEMISTRY
·
2014
·Vol. 16
·期 5
·2762-2770
·
IF 6.852
Zehui Zhang,Bing Liu,Kangle Lv,Jie Sun,Kejan Deng
In this study, we have successfully prepared a heterogeneous catalyst (K-10 clay-Mo) by the immobilization of bis(acetylacetonato) dioxo-molybdenum(VI) [MoO2(acac)2] on montmorillonite K-10 clay. The structure of the resultant catalyst was characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electronic microscopy (TEM), and Fourier transform infrared (FTIR) spectroscopy. The catalytic activity of K-10 clay-Mo was tested in the aerobic oxidation of 5-hydroxymethylfurfural (HMF). Although a molecule of HMF contains one hydroxyl group and one aldehyde group, to our surprise, the catalyst showed high catalytic activity for the oxidation of the aldehyde group of HMF into 5-hydroxymethyl-2-furancarboxylic acid (HMFCA). A variety of important reaction parameters such as the reaction temperature, catalyst amount, and solvent were explored. HMFCA could be obtained in a high yield of 86.9% with a HMF conversion of 100% after a short reaction time of 3 h in toluene. More importantly, the catalyst K-10 clay-Mo could be reused several times without a significant loss of its catalytic activity.
研究领域:
化学工程
·
催化材料
✓ 已验证
DOI
高影响因子
JOURNAL OF HETEROCYCLIC CHEMISTRY
·
2015
·Vol. 54
·期 1
·784-788
·
IF 0.787
Fang-Ming Wang,Dan Bao,Bing-Xiang Hu,Ze-Yu Zhou,Deng-Deng Huang,Li-Zhuang Chen,Yuan-Yuan Dan
A series of novel 10-substituted 3,6-diphenyl-9-aryl-3,4,6,7,9,10-hexahydroacridine-1,8(2H,5H)-dione derivatives 2 were synthesized by condensation of compounds 1 with amines, which the compounds 1 were synthesized by 5-phenylcyclohexane-1,3-dione and aromatic aldehydes according to Knoevenagel, Michael, and cyclization reactions in the presence of a very small amount of l-proline as catalyzed at room temperature. The structures of all the synthesized compounds were characterized by IR, 1H NMR, and MS. In addition, the molecular structures of compound 1a have been determined by single-crystal X-ray diffraction analysis.
研究领域:
有机化学
·
药物化学
·
生物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2019
·Vol. 43
·期 3
·569-576
·
IF 2.516
Ruonan Shuang,Mengdi Wang,Juanling Mao,Jiamei Zou,Yuhui Ping
10-Hydroxycamptothecin is a drug to cure various cancers. However, the 10-hydroxycamptothecin cannot be widely applied in clinics due to fast elimination and resistance of various cancers to the drug. Nevertheless, co-treatment with tetrandine is known to reverse the resistance of multi-drug resistant cancers, and may present an effective strategy to improve the efficacy of 10-hydroxycamptothecin. In order to improve the bioavailability and prolong the treatment time of the 10-hydroxycamptothecin in vivo, we prepared 10-hydroxycamptothecin-tetrandrine liposome complexes with 10-hydroxycamptothecin as the basic anticancer drug, tetrandrine and liposomes as carriers. In this article, an ultra-high performance liquid chromatography tandem mass spectrometry method for the analysis of 10-hydroxycamptothecin and tetrandrine in plasma has been developed, validated, and utilized to compare the pharmacokinetics of both drugs in the original dosage form and administered as liposome complexes. According to the pharmacokinetic parameters of mean residence time, half-life period and clearance rate, the 10-hydroxycamptothecin-tetrandrine liposome complexes prolongs the retention and circulation time of 10-hydroxycamptothecin in vivo, achieving a good sustained release effect. To the best of our current knowledge, the pharmacokinetic properties of 10-hydroxycamptothecin-tetrandrine liposome complexes in rats have not been reported yet. Our study can provide a helpful reference for further related study.
研究领域:
药理学
·
肿瘤学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2018
·Vol. 8
·期 9
·648
·
IF 3.504
Xueping Song,Shuang Yang,Xiuyu Liu,Min Wu,Yao Li,Shuangfei Wang
Acetylated nanofibrillated cellulose (ANFC)/acrylic resin ABPE-10 composite film was prepared by impregnating ABPE-10 into ANFC films under negative pressure, which can enhance properties of ANFC films by forming an interpenetrating polymer network structure between ABPE-10 and the ANFC film. The ANFC/ABPE-10 composite film met the high performance flexible organic light-emitting diode substrate requirement, even when the ANFC dosage was as high as approximately 70%. The transparency of films with different ANFC dosages significantly increased from 67% (42 µm) to 88% (45 µm), as determined by ultraviolet-visible analysis. The composite film inherited the properties of AFNC, with a low coefficient of thermal expansion and a ductile compact structure. The contact angles of ANFC films increased from 49.2° to 102.9° after dipping in ABPE-10. Additionally, the composite films had good surface smoothness and mechanical properties.Keywords:acetylated nanofibrillated cellulose;acrylic resins ABPE-10;composite films;flexible organic light-emitting device substrate;interpenetrating polymer network
研究领域:
材料科学
·
光学工程
✓ 已验证
DOI
PubMed
高影响因子
DENTAL MATERIALS
·
2022
·
IF 5.687
Xin Jin,Fei Han,Qi Wang,Xiaojun Yuan,Qiyue Zhou,Haifeng Xie,Lina Niu,Chen Chen
Objectives 10-Methacryloyloxydecyl dihydrogen phosphate (MDP) has been regarded as the most effective dentin-bonding monomer for more than 20 years. Although the dentin-bonding promoting effect of MDP has been well demonstrated, the mechanisms by which it benefits the stably of collagen within the adhesive–dentin hybrid layer are not currently fully understood. The objective of this study was to investigate the roles of MDP and its calcium salt in preserving the adhesive–dentin hybrid layer. Methods MDP-conditioned collagen was investigated by Fourier-transform infrared spectroscopy, Ultraviolet–visible spectroscopy, and molecular docking. The structural changes to the dentin surface upon acid-etching and MDP-conditioning were observed by SEM. X-ray diffraction and nuclear magnetic resonance were used to investigate the chemical interactions between MDP and HAp. The collagen-protecting effects of MDP and its Ca salt were investigated using in-situ zymography, rhMMP-9 colorimetric assay, hydroxyproline assay, and molecular docking. Results MDP forms a stable collagen-phosphate complex through hydrogen bonding with the collagen in dentin. Furthermore, it generates MDP-Ca salts that are deposited on the dentin collagen scaffold, protecting it from degradation. Moreover, both free MDP and the MDP-Ca salt inhibit matrix metallopeptidase and exogenous proteases, with the inhibitory effect of the calcium salt being significantly stronger than that of the free form. Significance MDP-based adhesives preserve the collagen within the hybrid layer by simultaneously improving collagen’s resistance to exogenous enzymes and inhibiting MMP activity, both of which contribute to the longevity of dentin–resin bonding.
研究领域:
牙科材料
·
牙科粘接
·
胶原生物学
·
酶抑制
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）