Systems Microbiology and Biomanufacturing
·
2025
·
IF 2.9
Li Zihan,Zhao Guihong,Zhang Dezhi,Tang Yaqun,Liu Geer,Wang Xiaoyuan
2′-Fucosyllactose is the most abundant human milk oligosaccharides and one of the three essential nutrients for infant growth. Corynebacterium glutamicum is one of the most common industrial fermentation bacteria but cannot synthesize 2′-fucosyllactose. In this study, C. glutamicum ATCC13032 was engineered for 2′-fucosyllactose production from fucose and lactose. The gene futC from Helicobacter pylori encoding α-1,2-fucosyltransferase was codon optimized and mutated at four amino acids (F40S/Q150H/C151R/Q239S). The modified gene futC and the gene fkp from Bacteroides thetaiotaomicron encoding fucokinase/GDP-fucose pyrophosphorylase were overexpressed in plasmid pEC and transformed into C. glutamicum , resulting in CW002. CW002 did not synthesize 2′-fucosyllactose possibly because the substrates fucose and/or lactose did not pass through the cell membrane. Therefore, the gene lacY encoding lactose permease and the gene fucP encoding fucose permease from Escherichia coli were overexpressed in plasmid pXTuf and transformed into CW002, resulting in CW006. CW006 did synthesize 2′-fucosyllactose as expected. It is interesting that the production of 2′-fucosyllactose was decreased or stopped when the expression combination of these four genes was changed, suggesting that the expression levels of the four genes in CW006 might have to well balanced. C. glutamicum CW006 produced 2.07 g/L 2′-fucosyllactose in a 2.4 L bioreactor.
研究领域:
微生物学
·
生物工程
·
分子生物学
·
食品科学
✓ 已验证
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2017
·
IF 4.798
Zhonghua Xue,Xiaoxia Fu,Honghong Rao,Xibin Zhou,Xiuhui Liu,Xiaoquan Lu
As efficient electron transfer mediator, various nanomaterials with smart performance have been explored in non-enzymatic sensor fabrication. We herein present an electron transfer mediator actuated electrochemical non-enzymatic sensor to assay nitrite, enabled by electrochemical reduced graphene oxide (ERGO) and 1-(2-pyridylazo)-2-naphthol (PAN). The composites of PAN-ERGO coating on electrode surface were successfully fabricated through a facile and green cyclic voltammetry technique. With the aid of ERGO nanosheets on the electrode surface, the electron transfer mediator of PAN nanostructures was easily immobilized and hybridized with ERGO, resulting in a remarkably synergistic effect between ERGO sensing substrate and the electrodeposited PAN coating for target electron transfer. The results demonstrated that the electrocatalytic current increased linearly with the nitrite concentration in the range of 7.49–1752.6 μM and 1752.6–3500 μM, with a good detection limit of 0.67 μM and 1.44 μM (signal-to-noise ratio, S/N = 3), respectively. The present work provide some new insights for the smart design of high efficiency electrocatalytic materials that based on the composites of electrochemical reduced graphene oxide and other substances.
研究领域:
分析化学
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材料科学
·
电化学传感器
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Feng Wang,Xianhong Bi,Yun Cui,Kewei Lin,Charles Brennan,Soottawat Benjakul,Gengsheng Xiao,Lukai Ma
Toxic compounds in fried food products attract increasing attention, but studies regarding the levels of common toxins, such as malondialdehyde (MDA), 4-hydroxy-2-hexenal (4-HHE), and 4-hydroxy-2-nonenal (4-HNE), in fried starch-based food (FSBF) are scarce. Herein, we investigated the formation and distributions of these aldehydes in FSBF, and the frying oil and FSBF retained higher levels of low-molecular-weight aldehydes. The highest aldehyde concentrations were observed in French fries. Simulated frying experiments revealed that the distributions of the toxic aldehydes in FSBF were mainly determined by the amylose content. Molecular dynamics simulations indicated that amylose interacted strongly with 4-HNE, but its interactions with MDA and 4-HHE were weaker. The migration of MDA, 4-HHE, and 4-HNE into FSBFs was primarily attributed to the interactions between the toxic aldehydes and amylose. This study should provide a novel basis for the control and elimination of toxic aldehydes in fried food.
研究领域:
食品安全
·
分子生物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemistry-An Asian Journal
·
2024
·
IF 3.5
Xinxin Dai,Zhen Tang,Xinyuan Yan,Shiyi Tao,Shanshan Wang,Yi Liu,Jiafeng Cao,Xiaolong Deng,Xin Bo
The exploring and developing non-precious transition metal-based catalysts for practical water electrolysis with the low cost, high efficiency and easy macroscopic preparation was still a challenge. Herein, Ni 3 S 2 /Ni(OH) 2 heterojunction with different sulfuration time was proposed and hydrothermally synthesized using a simple two-step approach, which served as a bifunctional electrocatalyst for water splitting in alkaline solution at industrial temperature. Among these catalysts, Ni 3 S 2 /Ni(OH) 2 -5h displayed the smallest overpotentials (237 mV@100 mA cm −2 and 360 mV@100 mA cm −2 ) for OER and HER at room temperature, along with low Tafel slopes of 62.0 mV dec −1 and 80.8 mV dec −1 respectively. Furthermore, working at high temperature Ni 3 S 2 /Ni(OH) 2 -5h exhibited even lower overpotential of 82 mV@100 mA cm −2 at 70 °C for OER and 325 mV@100 mA cm −2 at 60 °C for HER. The excellent performance was ascribed to the heterojunction accelerating the charge transfer, hierarchical interconnected structure promoting the mass transfer and the synergistic effect between the components of Ni 3 S 2 and Ni(OH) 2 . This work could provide a promising route for promoting the electrocatalytic performance of Ni-based catalyst with a simple sulfuration method for industrial water splitting, which could expand to other non-noble metal-based materials for enhancing the electrocatalytic activities.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CRYSTAL GROWTH & DESIGN
·
2024
·
IF 3.2
Huiqin Ma,Zhuxiang Mao,Wen Liu,Yahao Zhao,Peng Liu,Difu Zhan,Qian Fu,Yizhong Huang,Changcun Han
ZnIn2S4 (ZIS) is an N-type direct bandgap semiconductor material with a visible light response, which exhibits excellent photocatalytic water-splitting performance. To enhance ZIS-based photocatalysts, heterojunction construction and cocatalyst loading methods have been explored extensively. In this research, a Co9S8/ZnIn2S4 (CS/ZIS) heterojunction was successfully synthesized via a solvothermal method, and then Ni(OH)2 nanoparticles were successfully loaded on CS/ZIS by photodeposition, yielding the Ni(OH)2/CS/ZIS photocatalyst. Our findings reveal tight coupling of ZIS nanosheets with the CS surface, establishing an effectively constructed heterojunction structure that enhances interface charge transfer and light absorption. The synergistic effect of CS and Ni(OH)2 significantly boosts the photocatalytic hydrogen production of ZIS composites. The optimum hydrogen production rate of CS/ZIS is 10.06 mmol·h–1·g–1 and that of Ni(OH)2/CS/ZIS reaches 12.92 mmol·h–1·g–1, a 11.9-fold increase over pure ZIS, with exceptional light stability. Furthermore, we discuss the photocatalytic reaction mechanism based on the experimental and characterization results. This work presents a novel approach to designing highly efficient photocatalysts using ZnIn2S4, aimed at enhancing the conversion of solar energy into chemical energy.
研究领域:
材料科学
·
能源化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2016
·
IF 2.74
Junzhi Li,Junqiang Wang,Wei Zhao,Xiaoliang Li
Isobaric vapor-liquid equilibrium (VLE) data for the four systems n -heptane + 2-methylthiophene, 2,2,4-trimethylpentane + 2-methylthiophene, n -nonane + 2-methylthiophene, n -decane + 2-methylthiophene were measured at 90.00 kPa with a modified Rose-Williams still. Gas chromatography equipped with a flame ionization detector was used to analyze composition of the samples from the vapor-liquid equilibrium systems. All the VLE measurements passed the thermodynamic consistent test using the Herington method and the Van Ness test. The azeotropic behavior was found in the 2,2,4-trimethylpentane + 2-methylthiophene system. The experimental data were correlated by the Wilson and NRTL activity coefficient models and also compared with the original UNIFAC predictive model. The corresponding parameters for the three models were obtained. The results exhibited that the measured data got agree well with the calculated values using the Wilson, NRTL and original UNIFAC models.
研究领域:
化学工程
·
物理化学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yue Lu,Jiahui Lei,Haoran Dong,Linrui Zhong,Qiqi Fu,Qingqing Xie,Long Li,Fangyi Liang
Chlorophenols (CPs) pose potential threat to the environment and public health due to their persistence and high toxicity. Zero-valent iron coupled with microorganisms have been applied for the degradation of halogenated organic matter, which is considered as a remediation technology of increasing interest. However, few studies have attempted to investigate the effects of this coupled system on CPs degradation and to comprehensively unveil the governing mechanisms. In this study, we systematically compared the effects of the composite system of microscale zero-valent iron (mZVI) with different particle sizes and Desulfitobacterium hafniense DCB-2 (i.e. 3 μm mZVI + DCB-2, 5 μm mZVI + DCB-2, and 10 μm mZVI + DCB-2) on the process of bioreductive dechlorination of 2,3-dichlorophenol (2,3-DCP). Interestingly, the lower particle sizes of mZVI (3 μm and 5 μm) were more favorable for rapid 2,3-DCP bio-dechlorination due to the fact that mZVI with smaller particle size were less likely to agglomerate with strain DCB-2, which facilitated the transfer of electrons from strain DCB-2 to 2,3-DCP. Besides, the optimal particle size of mZVI (3 μm) was selected from the above experimental results to further explore the percentage generation of reduction products and the dechlorination pathway of 2,3-DCP in composite system. The results showed that the low toxicity product 2-chlorophenol (2-CP) accounted for 31.76 % of the composite system, while in the pure bacteria treatment this value was only 20.42 %. The mZVI and DCB-2 coupling strategy might provide a new idea and feasible solution for the green reduction of chlorinated phenols.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
RADIATION PHYSICS AND CHEMISTRY
·
2024
·
IF 2.8
Qin Wen,Shizong Wang,Jianlong Wang,Hai Chen,Yuedong Zhou
In this study, the degradation and mineralization of 2-chlorobiphenyl (2-PCB) by gamma irradiation were investigated in the presence of persulfate (PS), to examine the synergistic effect of radiation and persulfate oxidation. The results showed that the absorbed dose required for complete removal of 2-PCB decreased in the presence of PS. In addition, compared to a single irradiation system, the removal efficiency and mineralization of 2-PCB in deionized water , groundwater, and secondary effluent increased when irradiation combined with PS. 2-PCB at concentration of 4 mg L −1 to 8 mg L −1 could be efficiently degraded at a wide pH range (3.0–9.5) in irradiation-activated PS (irradiation/PS) system. The presence of 10 mM or 100 mM of Cl − , NO 3 − , CO 3 2− , and SO 4 2− in irradiation/PS system had no obvious influence on 2-PCB removal. 2-PCB was degraded under the combined action of sulfate radicals (SO 4 •− ) and hydroxyl radicals (·OH), in which ·OH contributed more. The irradiation/PS system is promising to treat PCB-containing wastewater.
研究领域:
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Battery Energy
·
2024
·
IF 9
Xiangchao Xu,Fen Qiao,Yanzhen Liu,Wenjie Liu
Cu(OH) 2 has the advantages of ease of structural regulation, good conductivity, and relatively low cost, making it a suitable candidate material for use as an electrocatalyst. However, its catalytic efficiency and stability still need to be improved further. Therefore, Cu(OH) 2 /Cu 2 S was successfully prepared on copper foam (CF) using the in situ growth and hydrothermal method. The structural characterization showed that sulfidation treatment induced transformation of Cu(OH) 2 /CF from smooth nanorods into a coral-like structure, which exposed more active sites of Cu(OH) 2 /Cu 2 S and enhanced the performance of electrocatalytic hydrogen evolution reaction (HER). Compared with Cu(OH) 2 , Cu(OH) 2 /Cu 2 S showed better alkaline HER performance, especially when the vulcanization concentration was 0.1 M, the overpotential of Cu(OH) 2 /Cu 2 S was 174 mV, and the reaction kinetics was 64 mv dec −1 at a current density of 10 mA cm −2 . In this work, the morphology and electronic structure of copper-based metal sulfide electrocatalysts were adjusted by sulfide treatment, which provided a new reference for improving HER performance.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2024
·
IF 4.1
Yanle Li,Dong Liang,Taisheng Zhu,Yumiao Zhou,Rushui Zheng,Na Zhu,Yongxiang Li,Duanlin Cao
Escaping from conventional hazardous methods, an innovative, acidic-free approach for the synthesis of nitro-heterocycles was realized through photocatalytic nitration using Pd-doped g-C 3 N 4 . In this method, chlorate nitrate was initially utilized as a reagent. Spectral analysis and electronic microscopy confirmed the successful doping of Pd nanoparticles (NPs) onto the g-C 3 N 4 surface, which subsequently enhanced the photocatalytic performance. The photogenerated electrons and Pd NPs played pivotal roles in the production of nitro-products. The reactive intermediate, NO 2+ , derived from nitrate, can be captured and elucidated through the shifts in cyclic voltammetry (CV) curves. Herein, the synthesis of 2-nitropyrrole and 2,5-dinitropyrrole was achieved through the nitration of chlorate nitrate in a friendly manner. Moreover, the composite photocatalyst could be readily isolated and recycled in next four tests, demonstrating its practicality and sustainability in photocatalytic applications.
研究领域:
有机化学
·
材料科学
·
环境化学
✓ 已验证
产品关联
DOI
高影响因子
Polymer Chemistry
·
2024
·Vol. 15
·期 21
·2148-2156
·
IF 4.1
Xiao-Yu Zhang,Hao-Liang Li,Bo Xiang,Xiang-Wei Liu,Shu Zhang,Yi-Xian Wu
A series of imidazolidin-2-iminato vanadium complexes VOCl2[1,3-R2-(CH2N)2CN], Vi1, R = 2,6-Me2C6H3; Vi2, 2,4,6-Me3C6H2; Vi3, R = 2,6-iPr2C6H3; Vi4, R = cyclohexanyl, were synthesized and fully characterized by NMR (1H, 13C and 51V) and elemental analysis. The copolymerizations of ethylene with propylene using the imidazolidin-2-iminato vanadium complexes were conducted in the presence of Et3Al2Cl3. The catalytic activity increased in the order of Vi4 < Vi1 < Vi2 < Vi3, probably due to the different electron donating natures of the imidazolidin-2-iminato ligands. Both high catalytic activity of 2.44 × 106 g copolymer per mol of V per h and incorporation ratio of propylene (up to 32.5 mol%) were afforded by using the Vi3/Et3Al2Cl3 catalytic system in the copolymerization of ethylene with propylene. An ethylene–propylene copolymer with ultra-high molecular weight (Mw = 2660 kg mol−1) could be also synthesized by using the Vi3/Et3Al2Cl3 catalytic system. Importantly, the highly efficient terpolymerizations of ethylene, propylene with 5-ethylidene-2-norbornene (ENB) were also achieved by using this catalytic system, affording ethylene–propylene–ENB terpolymers (EPDMs) with high contents of ENB units up to 9.8 mol% (29.8 wt%) and ultra-high molecular weight (Mw = 1521–2537 kg mol−1). This work offers a unique catalytic system of vanadium complexes for the synthesis of EPDM with both high content of ENB units and ultra-high molecular weight.
研究领域:
聚合物化学
·
材料科学
✓ 已验证
DOI
高影响因子
WATER RESEARCH
·
2018
·
IF 7.051
Zi-Bin Xu,Wen-Long Wang,Nan Huang,Qian-Yuan Wu,Min-Yong Lee,Hong-Ying Hu
2-Phosphonobutane-1,2,4-tricarboxylic acid (PBTCA) is an antiscalant that is widely used in reverse osmosis (RO) systems. Because of its high concentration in RO concentrate, eutrophication risk and anti-precipitation properties may affect subsequent treatments, therefore treatment strategies are needed to eliminate such substances. In this study, PBTCA was degraded by ozonation . The results show that PBTCA reacted with ozone molecules and hydroxyl radicals , with second-order rate constants of (0.12 ± 0.002) and (7.83 ± 1.51) × 10 8 L mol −1 s −1 , respectively. The phosphorus in PBTCA (P P ) was transformed into organic phosphorus except for PBTCA (P O ), and inorganic phosphorus (P I ); P O was further transformed into P I . The changes in the concentrations of these phosphorus forms were investigated by model simulation. Simulation showed that the rate of P P transformation into P O was 5.5 times higher than that into P I . PBTCA was ozonated much faster at alkaline pH than at acidic pH. This is ascribed to different amounts of ozone molecules and hydroxyl radicals, and their different reaction rates with PBTCA. Furthermore, anti-precipitation property was reduced during ozonation , as shown by the amounts and morphology changes of the precipitates. PBTCA concentration for 50% anti-precipitation (AP 50 ) did not change during ozonation, indicating that the transformation products generated during ozonation did not have anti-precipitation effects. Phosphorus in PBTCA was removed by ozonation–coagulation treatment. Total phosphorus and inorganic phosphorus were removed efficiently by using ferric chloride as a coagulant. The coagulants tended to bind with inorganic phosphorus to form flocs. Meanwhile, flocs were more easily to aggregate and precipitate as anti-precipitation effect was gradually removed, thus more phosphorus was removed. A combination of ozonation and coagulation removed PBTCA effectively and simultaneously reduced its anti-precipitation property and phosphorus.
研究领域:
环境科学
·
水处理工程
·
化学动力学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）