Journal of Central South University
·
2020
·Vol. 26
·期 11
·2974-2986
·
IF 1.249
Xu Si-quan,Pan Dong-hui,Xiao Guo-min
With the aim of achieving a high 5-hydroxymethylfurfural (HMF) yield from glucose with H-ZSM-5 catalyst at low cost, three inexpensive biphasic reaction systems, H 2 O—tetrahydrofuran (THF), H 2 O—2-methyltetrahydrofuran (MeTHF) and H 2 O’2-butanol, were discovered and proved to be particularly effective in promoting the formation of HMF from glucose over H-ZSM-5 zeolite. In order to determine the optimal process conditions, the effects of various experimental variables, such as reaction temperature, reaction time, catalyst dosage, volume of organic solvent, as well as inorganic salt type on glucose conversion to HMF in three systems were investigated in detail. It was found that under optimal reaction conditions, H 2 O—THF, H 2 O—2-butanol and H 2 O—MeTHF allowed the glucose dehydration process to achieve HMF yields of up to 61%, 59%, and 50%, respectively. Moreover, in the three biphasic systems, the H-ZSM-5 catalyst was also demonstrated to maintain excellent stability. Thus, the catalytic approach proposed in this paper can be believed to have potential prospects for industrially efficient and low-cost production of HMF.
研究领域:
化学工程
·
生物质转化
·
催化科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of CO2 Utilization
·
2022
·
IF 8.321
Mengmeng Ma,Yuchen Liu,Yuli Chen,Guohua Jing,Bihong Lv,Zuoming Zhou,Shihan Zhang
The large-scale deployment of non-aqueous amine solution for carbon dioxide (CO 2 ) capture is mainly limited by its high viscosity, even insoluble substances in saturated solution, which may result in equipment fouling and pipeline blockage. To avoid this problem, the polyamine 1,5-diamino-2-methyl-pentane (DA2MP) was screened as the main absorbent to ensure the high absorption load. While 2-amino-2-methyl-1-propanol (AMP) was used as the regulator to reduce the viscosity and eliminate insoluble substances, which dissolved in n-propanol (PrOH) to from the novel non-aqueous absorbent for CO 2 capture. The CO 2 absorption load of the novel absorbent was 0.95 mol·mol −1 , and the viscosity of saturated solution was only 15.00 mPa·s, remarkably lower than that of the solution without AMP regulation. The solution maintained 97% of its initial CO 2 absorption capacity after fourth regeneration cycle. The regulation mechanism was investigated using 13 C nuclear magnetic resonance (NMR) and quantum chemical calculations. It was proved that a strong and dense hydrogen bond network between DA2MP-carbamates was formed due to the self-deprotonation process, leading to insoluble products in the saturated DA2MP/PrOH solution. Through AMP regulation, there was another type of DA2MP-carbamates deprotonated by AMP formed in the solution with less hydrogen bond network, which could further react with PrOH to produce alkyl carbonates, achieving the elimination of insoluble products and reducing viscosity. Overall, the total regeneration energy consumption was 1.86 GJ·ton −1 CO 2 , only 50.27% of that of the benchmark monoethanolamine (MEA)-based solution, which would be a promising candidate for CO 2 capture.
研究领域:
化学工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Catalysis Science & Technology
·
2021
·Vol. 11
·期 13
·4439-4446
·
IF 6.119
Gaofei Liu,Xin Tong,Jialing Wang,Bin Wu,Jianlin Chu,Yongchan Jian,Bingfang He
2-Halogenated-2′-deoxyadenosines are important antiviral and anticancer drugs. However, natural enzymes display low activities in the biosynthesis of 2-halogenated-2′-deoxyadenosines, and the results are unsatisfactory because of the steric impediment created by the halogenated substituent group in purine. In this study, a semi-rational design was performed to enhance the activity of purine nucleoside phosphorylase from Aneurinibacillus migulanus AM007 (AmPNP) for the biosynthesis of 2-halogenated-2′-deoxyadenosines. Small and smart libraries of AmPNP were generated by stepwise site-directed evolution using iterative combinatorial mutations. The best AmPNP mutant, M3 (N233D/E191Q/Y190V/M249I), exhibited a satisfactory phosphorolysis activity towards 2′-deoxyadenosine, with a 144.75-fold improvement compared with that of wild-type AmPNP. In a coupled reaction of the M3 mutant and Brevibacillus borstelensis LK01 pyrimidine nucleoside phosphorylase, the conversion rates remarkably improved and reached 89.94% (cladribine) and 81.24% (2-fluoro-2′-deoxyadenosine) within 10 h, respectively. The substrate-binding cavity of the M3 mutant was reshaped to provide good accessibility for halogenated molecules, which led to a higher activity in the synthesis of 2-halogenated nucleosides. This directed evolution towards overcoming challenges in the biosynthesis of halogenated nucleosides may result in a remarkable economic impact on biotechnological nucleoside production.
研究领域:
生物技术
·
生物化学
·
药学
·
酶学
✓ 已验证
产品关联
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2024
·
IF 4.5
Ting Wang,Xiufeng Liao,Xiaodi Zhao,Kai Chen,Yangzhonghui Chen,Hui Wen,Dali Yin,Yuchen Wang,Bin Lin,Sen Zhang,Huaqing Cui
Mimicking the transition state of tryptophan (Trp) and O 2 in the enzymatic reaction is an effective approach to design indoleamine 2, 3-dioxygenase 1 (IDO1) inhibitors. In this study, we firstly assembled a small library of 2-substituted benzo-fused five membered heterocycles and found 2-sulfinyl-benzoxazoles with interesting IDO1 inhibitory activities. Next the inhibitory activity toward IDO1 was gradually improved. Several benzoxazoles showed potent IDO1 inhibitory activity with IC 50 of 82–91 nM, and exhibited selectivity between IDO1 and tryptophan 2, 3-dioxygenase (TDO2). Enzyme binding studies showed that benzoxazoles are reversible type II IDO1 inhibitors, and modeling studies suggested that the oxygen atom of the sulfoxide in benzoxazoles interacts with the iron atom of the heme group, which mimics the transition state of Fe-O-O-Trp complex. Especially, 10b can effectively inhibit the NO production in lipopolysaccharides (LPS) stimulated RAW264.7 cells, and it also shows good anti-inflammation effect on mice acute inflammation model of croton oil induced ear edema.
研究领域:
药理学
·
免疫学
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2020
·
IF 4.65
Weijun Liang,Senlin Wang,Yanxiu Zhang,Jian Gu,Kaida Zhu,Jing Zhong,Xinru Wang
β-Ni(OH) 2 with good crystallinity is grown on the surface of the flower-like α-Ni(OH) 2 to form a 3D α@β-Ni(OH) 2 material by a two-step hydrothermal method . The synthesized β-Ni(OH) 2 is nanometer size and strong alkali stability, while the α-Ni(OH) 2 has a large specific surface, high specific capacity and good electrical conductivity , so there are multiple synergistic effects between the β-Ni(OH) 2 coating and the α-Ni(OH) 2 core. Coupled with a 3D flower-like structure, the obtained α@β-Ni(OH) 2 electrode shows a high capacity of 431 mAh g −1 at a current density of 1 A g −1 . Furthermore, we use the α@β-Ni(OH) 2 electrode and the activated carbon (AC) electrode to assemble an asymmetric supercapacitor (ASC) device. This ASC shows the excellent energy density of 53 Wh kg −1 at a power density of 875 W kg −1 and capacity retention rate of 78% after 5000 cycles. Finally, metal hydride (MH) is used to replace the AC negative electrode, and a cell with a high energy density of 100.17 Wh kg −1 at a power density of 424.75 W kg −1 is obtained. The results show that α@β-Ni(OH) 2 electrode has better electrochemical performance than commercial electrodes and has application prospects in energy storage.
研究领域:
材料科学
·
能源存储与转换
✓ 已验证
产品关联
DOI
高影响因子
Desalination and Water Treatment
·
2015
·
IF 1.173
Ben Zhang,Juan Li,Xiaogai Lv,Yuanchen Cui,Ying Xu
Polyaspartic acid/2-amino-2-methyl-1,3-propanediol graft copolymer, a novel non-phosphorus scale and corrosion inhibitor, was synthesized and characterized by infrared spectroscopy. Its performance was evaluated by static scale inhibition method and dynamic corrosion testing. It was found that the graft copolymer showed excellent inhibition ability against CaCO3, CaSO4, and Ca3(PO4)2 scales and a definite inhibition corrosion performance as well. The inhibition efficiency was close to 99.9% against CaCO3, CaSO4, and Ca3(PO4)2 when the inhibitor was at an appropriate concentration. The inhibitor had still possessed higher antiscale efficiency against CaCO3 with increasing concentration of Ca2+ and heating time. In addition, The graft copolymer could change the structure of crystals CaCO3, CaSO4, and Ca3(PO4)2 by observation of scanning electron microscopy.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Zimin Cai,Fangqin Fu,Sifeng Zhu,Jibin Wu,Xu Han,Shuai Jiang,Zhiyong Qian,Cheng Wang
The pharmacokinetic behavior of nanocarrier in vivo has been confirmed to be greatly influenced by the dynamic protein corona covered its surface, which also affects the ultimate clinical efficacy. Herein, we explored one novel polymersomes based on Poly(2-methyl-2-oxazoline) polymers (PMOXA 5 -PDBH 13 -PMOXA 5 ) with excellent drug-carrying capacity and specific protein corona manipulation. Compared to PEGylated nanoparticle (NP), the polymersomes (PC-SOM) provided a considerable advancement in therapeutic efficiency, which can selectively accumulate in tumor tissues, enhancing cellular internalization via specific proteins covering its surface such as fibronectin and moesin. Notably, in vivo antitumor effect showed that doxorubicin-loaded PC-SOM exhibited superior inhibitory rate 75.5% than 48.24% of PEGylated NP. Furthermore, through protein corona analysis we found that PC-SOM enhanced the adsorption of apolipoprotein A-I while suppressed the adsorption of complement C3 and immunoglobulin in serum from healthy volunteers and human breast cancer volunteers. These elaborated the reasons of longer circulation times and superior tumor-targeting capabilities of PC-SOM. Our work provided a promising nanoagent with novel surface-design by the dynamic protein corona for excellent pharmacokinetic behavior and antitumor effect.
研究领域:
生物医学工程
·
药理学
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
RESEARCH ON CHEMICAL INTERMEDIATES
·
2013
·Vol. 41
·期 5
·3243-3260
·
IF 0.88
Fa HuanBao,Zhou JingTing,Zhang Dong,Yin Wei,Zhang HaiFeng,Huo DanQun,Hou ChangJun,Luo XiaoGang,Mao YaLi,Zhang Jin
A novel 4-(2-{[6-(1,1-dicyanoprop-1-en-2-yl)naphthalen-2-yl](methyl)amino} ethoxy)-4-oxobutanoic acid ( 5 ) fluorescent probe for β-amyloids was synthesized by catalytic acylation using 4-dimethylaminopyridine between succinic anhydride and (1-{6-[(2-hydroxyethyl)(methyl) amino]-2-naphthyl}ethylidene)malononitrile ( 4 ). The structures of all compounds were identified by proton nuclear magnetic resonance spectroscopy, infrared spectroscopy, mass spectrometry, and ultraviolet–visible (UV–Vis) spectroscopy. The UV–Vis and fluorescence spectra of 1-{6-[(2-hydroxyethyl)(methyl) amino]-2-naphthyl}ethan-1-one ( 3 ), 4 , and 5 in solvents with different polarities were investigated, and the effects of solvent polarity on the optical properties of the three compounds were studied. The objective product 5 showed high binding affinities toward Aβ(1–40) aggregates in vitro ( K
d = 29.4 nmol/L) by fluorophotometry. This study provides a powerful fluorescent probe for the molecular diagnosis of Alzheimer’s disease.
研究领域:
化学
·
生物化学
·
神经科学
·
分子生物学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2013
·
IF 5.695
Shao-Ting Wang,Wei Huang,Wei Lu,Bi-Feng Yuan,Yu-Qi Feng
A novel TiO2-based SPE strategy was developed for eliminating normal ribonucleosides before mass spectrometry (MS) analysis of 2′-deoxynucleosides and 2′-O-modified ribonucleosides. The chromatographic research for the retention behavior of ribonucleosides and 2′-deoxynucleosides on TiO2 materials was investigated using TiO2 separation column. The results indicated a specific affinity interaction mechanism between TiO2 and cis-diol-containing ribonucleosides, and the interaction was proved effective even under a wide range of pH conditions and salt concentrations. Benefiting from these features, a TiO2-based solid phase extraction (SPE) method was developed for highly efficient elimination of RNA contamination from genomic DNA. Compared with the widely used enzymatic digestion method, the proposed TiO2-based SPE method showed much more efficiency for the removal of RNA as well as provided high recoveries for the 2′-deoxynucleosides. In addition, the sample processing time is dramatically shortened using the TiO2-based SPE method (∼5 min) compared to the traditional enzymatic digestion method (∼12 h). Finally, the purification of 2′-O-methylated ribonucleosides from RNA was successfully achieved in HeLa cells by the TiO2-based SPE method, which provided a proof-of-concept for the purification of relevant modified ribonucleosides from bulky normal ribonucleosides. Taken together, this strategy developed in the current study offers a promising option to purify 2′-deoxynucleosides/2′-O-modified ribonucleosides for their sensitive and accurate determination by eliminating normal ribonucleosides in biological samples.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2022
·
IF 5.545
Li-Na Ke,Ling-Qi Kong,Xiu-Lian Zhu,Feng-Xu Wu,Qin-Hua Chen,Bin Li,Yun Dong,Hong-Mei Wang,Xiao-Hua Zeng
We have synthesized Rhopaladins’ analog (2E,4E)-4-chlorobenzylidene-2-(4-chlorostyryl)-N-cyclohexyl-1-(4-fluorophenyl)-5-oxopyrrolidine-2-carboxamide (RPDPRH) via a highly facile, inexpensive and green approach and verified the structural superiority of compound RPDPRH through molecular docking. Moreover, we further detected the anti-proliferation, apoptosis and HPV E6/E7 effects of RPDPRH on CaSki cells. Finally, we confirmed that compared with the previous compound (E)-N-(tert-butyl)-2-(4-chlorobenzoyl)-4-(4-fluorobenzylidene)-1-isopropyl-5-oxopyrrolidine-2-carboxamide (RPDPB), RPDPRH could better inhibit proliferation, induce apoptosis, and down-regulate HPV E6/E7 mRNA expression on Caski cells. And preliminary RT-PCR experiments have demonstrated that RPDPRH also could affect the expression of Bcl-2, Bax and Caspase-3 mRNA in Caski cells. In summary, RPDPRH has potential as an effective agent against cervical cancer and will play an important role in our subsequent research.Keywords: 4-arylidene-5-oxopyrrolidine, green synthesis, molecular docking, anti-tumor activity, apoptosis
研究领域:
化学
·
药理学
·
肿瘤学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Shan-Shan Yang,Qing-Yun Lv,Juan Fu,Tian-Yi Zhang,Yi-Si Du,Xin-Juan Yang,Le Zhou
As our further research, a series of new 7-chloro-9-methyl-2-phenyl-3,4-dihydro-β-carbolin-2-iums were designed and synthesized. Twelve compounds were found with excellent inhibition activity in vitro on three to five out of six phytopathogenic fungi, superior to standard drugs thiabendazole and/or azoxystrobin. Especially, 18 displayed the highest activity against three out of the fungi and the highest comprehensive activity for all of the fungi. The test in vivo revealed that 18 at 50 μg/mL was able to completely control Physalospora piricola infections in apples over 8 days. Scanning/transmission electron microscopic observations found that 18 could damage the hyphal integrity and cell membrane structure of P. piricola. The safety evaluation showed that 18 had no effect on the germination rate of cowpea seed at ≤200 μg/mL. The SAR revealed that the combination of 7-Cl and 2′- or 4′-alkyl is conducive to improvement of the activity. Thus, 7-chloro-9-methyl-2-phenyl-3,4-dihydro-β-carbolin-2-ium is a promising antifungal lead scaffold.
研究领域:
植物病理学
·
药物化学
·
农学
✓ 已验证
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2020
·
IF 5.88
Chengcheng Yin,Xiaoshi Jia,Qin Zhao,Zifan Zhao,Jinyang Wang,Yufeng Zhang,Zhi Li,Hongchen Sun,Zubing Li
Boron-containing mesoporous bioactive glass (B-MBG) scaffolds could be capable of promoting osteogenesis by activating Wnt/β-catenin signaling pathway during the process of bone defect repair. Despite this, more involving molecular controls are still largely unclear. In the present study, we identified that the downstream of Wnt/β-catenin signaling pathway named transcription factor 7-like 2 (TCF7L2) served as a key effector to promote boron-induced bone regeneration and osteogenesis through lipocalin 2 (LCN2). TCF7L2 was highly expressed in osteoblasts when treated with B-MBG scaffold extraction than MBG. LCN2, as a secreted bone factor, positively affected osteogenic differentiation of MC3T3-E1 and osteogenesis in vivo , which could be induced by TCF7L2. In addition, interference of TCF7L2 decreased the osteogenic differentiation of osteoblasts. Finally, we identified that rLCN2 could rescue the poor ability of osteogenic differentiation of MC3T3-E1 whose Tcf7l2 gene was knocked down by lentiviral transfection of shRNA. Our findings provide some new insights into the molecular controls of boron-associated bone regeneration and potential therapeutic targets for the treatment of bone defects.
研究领域:
生物医学工程
·
分子生物学
·
骨科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）