TALANTA
·
2018
·
IF 4.244
Wen-Hua Ji,Yu-Shuang Guo,Xiao Wang,D.-S. Guo
An accurate, rapid, and sensitive method for the determination of risperidone and 9-hydroxyrisperidone in urine samples was developed by combining water-compatible magnetic molecularly imprinted solid-phase extraction with high performance liquid chromatography. Several variables relating to the efficiency of magnetic solid phase extraction were optimized, including the amount of adsorbent, adsorption time, type of elution solvent, and desorption time. The analytical performance of this method was validated under the optimized conditions. The linearity for risperidone and 9-hydroxyrisperidone was obtained in the range 1–2000 ng mL −1 with correlation coefficient ≥ 0.991. Limits of detection of risperidone and 9-hydroxyrisperidone are 0.21 ng mL −1 and 0.24 ng mL −1 , respectively. Recoveries at three spike levels (10, 100, and 1000 ng mL −1 ) ranged from 94.6% to 102.4% with relative standard deviations (%) ≤ 5.3. These results confirmed that this method can be successfully and facilely used to analyze the multi-residues of risperidone and 9-hydroxyrisperidone in urine samples with high efficiency and good sensitivity.
研究领域:
分析化学
·
药物分析
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2021
·
IF 5.316
Xiaomeng Ma,Jialun Du,Xueli Ji,Lintao Liu,Haitao Wu,Hideo Kimura,YiZhong Lu,Zhenxing Yue
In this work, Nd 2 (Zr 1- x Sn x ) 3 (MoO 4 ) 9 (0.02 ≤ x ≤ 0.1) (NZSM) ceramics were successfully obtained through conventional solid-state route. Phase analysis, microstructure, and properties were investigated scientifically by XRD , SEM, and network analyzer, respectively. XRD results revealed that all samples with different substitution amounts of Sn 4+ formed the pure phase with R-3c space group. The permittivity was related to the polarizability and the bond of Zr2-O5, the bond of Nd-O2 possessed a positive influence on Q×f . The reduced octahedral distortion and increased bond energy made the temperature coefficient more stable. Infrared reflection spectrum results showed that the permittivity was mainly caused by the absorption of phonon oscillation. The optimum properties ( ε r = 10.34, Q×f = 74,795 GHz, τ f = −13.55 ppm/°C) were obtained when x = 0.02 sintered at 750 °C.
研究领域:
材料科学
·
陶瓷
·
微波介电材料
·
固态化学
✓ 已验证
DOI
高影响因子
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
·
2017
·Vol. 28
·期 10
·1866-1873
·
IF 1.198
Yin Qinhong,Zhu Yanqin,Yang Yaling
Oleic acid modified Fe3O4 nanoparticles were proposed for magnetic solid-phase extraction coupled with ultra high-performance liquid chromatography-tandem mass spectrometry to determine risperidone (RISP) and 9-hydroxyrisperidone (9OHR) in biological samples. The effects of various experimental parameters including adsorbent amount, pH, adsorption time, eluent type and concentration were systematically investigated. Under optimal conditions, the calibration curve was linear within the concentration range of 0.2-200 ng mL-1 with the correlation coefficient (r) of 0.9962 and the lower limit of quantification was 0.06 ng mL-1 for RISP; the calibration curve was linear within the concentration range of 0.2-200 ng mL-1 with the correlation coefficient of 0.9956 and the lower limit of quantification was 0.04 ng mL-1 for 9OHR. The extraction recoveries were over 90.0% with relative standard deviation less than 5.0%. All these results suggested that magnetic extraction method can be used for enrichment and quantification of RISP and 9OHR in biological samples.
研究领域:
分析化学
·
药物分析
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产品关联
DOI
高影响因子
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
·
2021
·
IF 1.312
Yan Du,Jinhua Hu,Zhixiong Hu,Weinong Zhang,Yutang Qi,Yanpeng Zhang,Xiaomin Li,Yi Liu
In this paper, a sensitive method for the determination of 3-chloro-1,2-propanediol (3-MCPD) by high performance liquid chromatography-fluorescence detection (HPLC-FLD) was established, in which 9-(2-Hydroxypropyl) adenine (HP-Ade) was selected as the fluorescent derivatization reagent because of its good water solubility and hydrophobicity. The principle of the method lies in the origination of chloroacetaldehyde with the periodate oxidation of 3-MCPD and its' fluorescence derivatization by HP-Ade to produce a strong fluorescent substance, name as 1-N6-vinylidene-9-(2-hydroxypropyl)adenine (ε-HP-Ade), used for quantitative determination of 3-MCPD by HPLC-FLD. Some mobile phase conditions and incubation parameters related with the method features were studied and optimized. Under the optimal conditions, the method shows high sensitivity, good linearity (r2 = 0.999) and good repeatability (RSD% between 1.04% and 2.55%), the detection limit (LOD) and quantification limit (LOQ) were 0.02 ng/mL and 0.06 ng/mL respectively. It has been successfully applied for the determination of various edible vegetable oil samples, the chromatogram shows symmetrical peak shape, perfect separation and very “clean” profiles. In comparison with gas chromatography-mass spectrum (GC-MS), the pretreatment process of the proposed method was totally operated in aqueous phase, the instrument requirements and cost are relatively lower, thus it will provide another choice for the routine analysis of 3-MCPD.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Huangmei Zhou,Yu Zhao,Xihang Chen,Sanjun Zhang
pH is a fundamental physiological parameter related to myriad applications, therefore great efforts are continuously made to develop high performance pH indicators. Here, we exhaustively investigated the pH response of an acridine analog, 9-acridinecarboxylic acid with steady state absorption and fluorescence spectroscopies, picosecond resolved fluorescence spectroscopy, and femtosecond resolved transient absorption spectroscopy. The fluorescence intensity ratio (R 450/525 ) of 9-acridinecarboxylic acid showed a 7-fold change in the physiological pH range (pH ∼ 5–8), and it can be served as an excellent ratiometric and lifetime pH indicator. Analysis of the picosecond and femtosecond-resolved spectra combined with theoretical calculation revealed that 9-acridinecarboxylic acid existed in zwitterionic form at acidic condition with emission centered at 480 nm and lifetime of about 2.1 ns and anionic form at alkaline condition with emission centered at 430 nm and lifetime of about 6.6 ns. As pH decreased, the protonation of heterocyclic N atom resulted in the redshift of fluorescence peak and shortening of the fluorescence lifetime. Moreover, 9-acridinecarboxylic acid exhibited good selectivity, photostability and excellent biocompatibility and was successfully applied for intracellular pH imaging by ratiometric fluorescence.
研究领域:
分析化学
·
生物物理学
·
光谱学
·
荧光成像
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Scientific Reports
·
2018
·Vol. 8
·期 1
·1-18
·
IF 4.122
Zhou Bohang,Zhang Bingyu,Li Xingqiang,Liu Xiuxiu,Li Hui,Li Ding,Cui Zhiming,Geng Huiling,Zhou Le
A series of 2-aryl-9-methyl- β -carbolinium bromides (B) were synthesized and explored for anti-acetylcholinesterase (AChE) activities in vitro , action mechanism and structure-activity relationship. All the compounds B along with their respective 3,4-dihydro intermediates (A) presented anti-AChE activity at 10 μM. Thirteen compounds B showed the excellent activity with IC 50 values of 0.11 – 0.76 μM and high selectivity toward AChE relative to butyrylcholinesterase (BChE), superior to galantamine (IC 50 = 0.79 μM), a selective AChE inhibitor drug. Kinetic analysis showed that the action mechanisms of both compounds B and A are a competitive inhibition model. Structure-activity relationship analyses showed that the C = N + moiety is a determinant for the activity. Substituents at 6, 7 or 4′ site, the indole-N-alkyl and the aromatization of the C-ring can significantly improve the activity. Molecular docking studies showed that the compounds could combine with the active site of AChE by the π-π or cation-π action between the carboline ring and the phenyl rings of the residues, and the β-carboline moiety is embedded in a cavity surrounded by four aromatic residues of Trp86, Tyr337, Trp439 and Tyr449. The present results strongly suggest that the para-position of the D-ring should be a preferred modification site for further structural optimization design. Thus, 2-aryl-9-methyl- β -carboliniums emerged as novel and promising tool compounds for the development of new AChE inhibitor agents.
研究领域:
药理学
·
有机化学
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2022
·Vol. 10
·期 11
·2865-2876
·
IF 7.59
Mengyue Pei,Huiyang Li,Yingwei Zhu,Jian Lu,Chuangnian Zhang
Liver cancer is the most common malignant tumor and liver cancer immunotherapy has been one of the research hotspots. To induce antigen-specific antitumor immune responses against liver cancer, we developed antigen and adjuvant co-delivery nanovaccines (APPCs). Polyanionic alginate (ALG) and polycationic polyethyleneimine (PEI) were utilized to co-deliver a glypican-3 peptide antigen and an unmethylated cytosine-phosphate-guanine (CpG) adjuvant by electrostatic interactions. A cellular uptake study confirmed that APPC could promote antigen and adjuvant uptake by dendritic cells (DCs). Importantly, APPC facilitated the endosomal escape of the peptide for antigen delivery into the cytoplasm. In addition, APPC showed significant stimulation of DC maturation in vitro. APPC could also efficiently prime DCs and induce cytotoxic T lymphocyte responses in vivo. The in vitro cell viability assay and the in vivo histocompatibility showed that APPC was non-toxic within the tested concentration. This study demonstrates that the peptide antigen and the CpG adjuvant co-delivery nanovaccine have potential applications in liver cancer immunotherapy.
研究领域:
免疫学
·
肿瘤学
·
分子生物学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Central Science
·
2023
·
IF 18.2
Ruoxi Wang,Yu Wang,Yuting Niu,Danqing He,Shanshan Jin,Zixin Li,Lisha Zhu,Liyuan Chen,Xiaolan Wu,Chengye Ding,Tianhao Wu,Xinmeng Shi,He Zhang,Chang Li,Xin Wang,Zhengwei Xie,Weiran Li,Yan Liu
Maintaining the stemness of bone marrow mesenchymal stem cells (BMMSCs) is crucial for bone homeostasis and regeneration. However, in vitro expansion and bone diseases impair BMMSC stemness, limiting its functionality in bone tissue engineering. Using a deep learning-based efficacy prediction system and bone tissue sequencing, we identify a natural small-molecule compound, dihydroartemisinin (DHA), that maintains BMMSC stemness and enhances bone regeneration. During long-term in vitro expansion, DHA preserves BMMSC stemness characteristics, including its self-renewal ability and unbiased differentiation. In an osteoporosis mouse model, oral administration of DHA restores the femur trabecular structure, bone density, and BMMSC stemness in situ. Mechanistically, DHA maintains BMMSC stemness by promoting histone 3 lysine 9 acetylation via GCN5 activation both in vivo and in vitro. Furthermore, the bone-targeted delivery of DHA by mesoporous silica nanoparticles improves its therapeutic efficacy in osteoporosis. Collectively, DHA could be a promising therapeutic agent for treating osteoporosis by maintaining BMMSC stemness.
研究领域:
生物医学工程
·
干细胞生物学
·
药理学
·
组织工程
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Weilu Liu,Haifeng Li,Shangmin Yu,Jiaxing Zhang,Weihua Zheng,Liting Niu,Gengen Li
In this work, we reported the synthesis of 3, 6-diamino-9-ethylcarbazole and its application as a new monomer for preparation of molecularly imprinted polymer (MIP) electrochemical sensor . The as prepared MIP sensor exhibited ultrahigh sensitivity and selectivity for the detection of 17-β-estradiol in attomolar levels (1 × 10 –18 mol L −1 ). The sensor works by detecting the change of the interfacial impedance that is derived from recognition of 17-β-estradiol on the MIP layer. The MIP sensor based on 3, 6-diamino-9-ethylcarbazole monomer revealed better performance than that of unmodified carbazole monomer. The monomer/template ratio, electropolymerization scanning cycles, and the incubation pH values were optimised in order to obtain the best detection efficiency. Under the optimised condition, the MIP sensor exhibits a wide linear range from 1 aM to 10 μM (1 × 10 –18 ̶ 1 × 10 −5 mol L −1 ). A low detection limit of 0.36 aM (3.6 × 10 –19 mol L −1 ) and a good selectivity towards structurally similar compounds were obtained. The proposed MIP sensor also exhibits long-term stability and applicability in human serum samples. These advantages enabled this MIP sensor to be a promising alternative of electrochemical sensor and may be extended to detection of other endogenous compounds .
研究领域:
分析化学
·
生物医学工程
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BMC Cardiovascular Disorders
·
2024
·Vol. 24
·期 1
·1-13
·
IF 2
Liu Hai-Hua,Wei Wei,Wu Fei-Fei,Cao Lu,Yang Bing-Jie,Fu Jia-Ning,Li Jing-Xia,Liang Xin-Yue,Dong Hao-Yu,Heng Yan-Yan,Zhang Peng-Fei
Background Hyperlipidemia damages vascular wall and serves as a foundation for diseases such as atherosclerosis, hypertension and stiffness. The NOD-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome is implicated in vascular dysfunction associated with hyperlipidemia-induced vascular injury. Sodium tanshinone IIA sulfonate (STS), a well-established cardiovascular protective drug with recognized anti-inflammatory, antioxidant, and vasodilatory properties, is yet to be thoroughly investigated for its impact on vascular relaxant imbalance induced by hyperlipidemia. Methods In this study, we treated ApoE-knockout (ApoE-/-) mouse with STS and assessed the activation of the NLRP3 inflammasome, expression of MMP2/9, integrity of elastic fibers, and vascular constriction and relaxation. Results Our findings reveal that STS intervention effectively preserves elastic fibers, significantly restores aortic relaxation function in ApoE-/- mice, and reduces their excessive constriction. Furthermore, STS inhibits the phosphorylation of spleen tyrosine kinase (SYK), suppresses NLRP3 inflammasome activation, and reduces MMP2/9 expression. Conclusions These results demonstrate that STS protects vascular relaxation against hyperlipidemia-induced damage through modulation of the SYK-NLRP3 inflammasome-MMP2/9 pathway. This research provides novel insights into the mechanisms underlying vascular relaxation impairment in a hyperlipidemic environment and uncovers a unique mechanism by which STS preserves vascular relaxation, offering valuable foundational research evidence for its clinical application in promoting vascular health.
研究领域:
心血管药理学
·
炎症
·
血管生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2019
·
IF 6.638
Na Li,Liqun Yang,Chenglin Pan,Phei Er Saw,Meng Ren,Biyun Lan,Junfeng Wu,Xiaoyi Wang,Tingting Zeng,Liyan Zhou,Li-Ming Zhang,Chuan Yang,Li Yan
s The anomalous high expression of matrix metalloproteinase 9 (MMP-9) is one important factor that impedes diabetic wound healing. Therefore, inhibition of MMP-9 expression in a diabetic wound could be a feasible method to promote wound healing. In this study, we studied the possibility of self-therapy using wound dressings that contain bacterial cellulose-hyperbranched cationic polysaccharide (BC-HCP) derivatives that encapsulate siRNA (BC-HCP/siMMP-9) and have controlled release properties. Herein, we used four HCPs (Gly-DMAPA, Gly-D4, Amyp-DMAPA, Amyp-D4) as gene carriers. Our results showed that all HCP derivatives were minimally toxic to cells in vitro , while the cationic properties of HCP could be used as a complexation agent for MMP-9 siRNA (siMMP-9). Upon exposure to bacterial cellulose (BC), the BC slowly released HCP/siMMP-9. The released siMMP-9 effectively reduced the gene expression and protein levels of MMP-9 in a human immortalized epithelial cell line (HaCAT) and in diabetic rat wounds. Inhibition of MMP-9 in the wounds of diabetic rats resulted in a significant enhancement of wound healing, suggesting that the BC-HCP/siMMP-9 composite dressing could be used as a safe and effective dressing to promote wound healing in diabetic rats. Statement of significance In this work, we evaluated the possibility of using bacterial cellulose-hyperbranched cationic polysaccharide derivatives (BC-HCP) as a self-therapeutic wound dressing with siRNA encapsulated and controlled release properties. Our results showed that the BC-HCP/siMMP-9 composite dressing slowly released HCP/siMMP-9. The released siMMP-9 effectively reduced the gene expression and protein level of MMP-9 in human immortalized epithelial cell line and in the wound of diabetic rats. The BC-HCP/siMMP-9 composite dressing promoted diabetic wound healing by the unique nanostructure of BC and by releasing siMMP-9 for specific MMP-9 inhibition. Therefore, it could be used as a safe and effective dressing to promote wound healing in diabetic rats. This is the first evidence on the study of using BC as a dressing composite by encapsulating HCP/siRNA complexes for efficient RNAi gene silencing for better wound healing in diabetic rats.
研究领域:
生物医学工程
·
药剂学
·
伤口愈合
·
糖尿病
·
基因治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR NEUROBIOLOGY
·
2024
·
IF 4.6
Wang Ajun,Li Nan,Zhang Nan,Liu Jian,Yang Tao,Li Dongxue,Li Changwen,Li Rui,Jiang Tongcui,Xia Chengyu
The pathogenesis and development of Moyamoya disease are still unclear. This study aimed to investigate the effect of desmoglein-2 (DSG2) on Moyamoya disease and determine the inhibitory effect of DSG2 in vascular remodeling in Moyamoya disease. RNA sequencing, immunohistochemistry (IHC), and western blotting were used to detect the expression of DSG2 in the superficial temporal artery (STA) tissues of Moyamoya disease. The association between DSG2 and endothelial cells’ biological activities was investigated by cell counting kit-8 (CCK-8), migration assay, tube formation assay, flow cytometry with Annexin V-FITC/PI staining, and TUNEL apoptotic cell detection kit. Pathways affected by overexpression or knockdown of DSG2 were identified in endothelial cells. The expression of DSG2 in the STA tissues of Moyamoya disease was lower than that in normal controls. Overexpression of DSG2 inhibits the proliferation and migration but promotes apoptosis in endothelial cells, and low DSG2 levels result in impaired angiogenesis. In addition, there was an interaction between DSG2 and MMP-9, and DSG2 acted through the PI3K signaling in endothelial cells. Our results indicate that DSG2 affects PI3K signaling in vascular endothelial cells, and MMP-9 is involved in DSG2-mediated vascular changes in Moyamoya disease.
研究领域:
神经病学
·
血管生物学
·
细胞信号
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）