Scientific Reports
·
2024
·Vol. 14
·期 1
·1-10
·
IF 3.8
Li Jiawei,Cheng Yi,Huang Yingchun,Xu Lixin,Zhang Kun,Yan Fei,Chen Jian,Lang Chunhui
Alkaline phosphatase (ALP) is a reliable biomarker for various diseases, making the development of new detection methods highly significant. Herein, we report a simple and accurate dual-signal optical detection strategy for measuring ALP activity across the entire clinical range in adults. Based on this, we designed a smartphone app to eliminate the limitations of large instruments, enabling real-time field detection. In this work, ALP catalyzes the conversion of the phosphate monoester of 2-phospho-L-ascorbic acid (PAA) into ascorbic acid (AA), a compound with strong reducing properties. AA facilitates the in-situ reduction of Ag + to form Au@Ag core-shell nanoparticles, accompanied by significant changes in the ultraviolet-visible absorption spectra and color. These changes allow for the qualitative and quantitative detection of ALP activity. In UV absorption spectroscopy and colorimetric detection, the signal shows good linearity with ALP activity concentrations, with detection ranges of 0 U/L to 200 U/L (R 2 =0.9961) and 25 U/L to 250 U/L (R 2 =0.9771), respectively. Subsequent selective analysis and serum sample analysis demonstrated the high selectivity and accuracy of the proposed method, highlighting its potential for field application.
研究领域:
生物标志物检测
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纳米技术
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医学诊断
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移动健康应用
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DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Chunrong Li,Yuzhi Xu,Si-Yang Liu,Yanfei Zhang,Wen Yin,Zong Dai,Xiaoyong Zou
Ascorbic acid (AA) and glutathione (GSH), the most abundant intracellular reductive substances, have been widely used as biomarkers for cancer cells identification. The current methods relying on imaging of AA or GSH alone to identify cancer cells may cause systematic errors, since a mutual conversion relationship exists between AA and GSH. In this work, we propose a fluorescent nanosensor for the simultaneous imaging of intracellular reductive substances including AA and GSH. Biocompatible fluorescent silicon nanoparticles (SiNPs) with rich surface amine and carboxyl groups were synthesized. The fluorescence of the SiNP was initially quenched by chelation of Fe 3+ ions, forming SiNP/Fe 3+ complex as the fluorescent nanosensor. Upon the redox reaction with reductive substances, the nanosensor showed sensitively fluorescent recovery. Moreover, benefited from the efficient cellular uptake of the SiNP/Fe 3+ and the overexpressed intracellular reductive substances in cancer cells, the fluorescent nanosensor was used to accurately identify the human breast carcinoma (MCF-7) cells from normal mammary epithelial (MCF-10A) cells by imaging of intracellular AA and GSH simultaneously. This strategy would be promising in imaging-guided precision cancer diagnosis.
研究领域:
癌症生物学
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纳米技术
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生物医学工程
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细胞生物学
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荧光成像
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产品关联
DOI
PubMed
高影响因子
COLLOID AND POLYMER SCIENCE
·
2026
·
IF 2.9
Sun Zhiguo,Wang Honglong,Wang Xiaoguang,Li Jiali,Lin Qinghua,Tian Fuliang
EB irradiation is widely used for starch modification. Nevertheless, modification mechanism and level are blurry especially at a higher dose irradiation, restraining deep functional material design. In this work, pure corn starch was irradiated by an EB in air with dose up to 500 kGy. Then, microstructural and physicochemical property variations were explored via FT-IR, XPS, 13 C-NMR, GPC, XRD, SEM, whiteness, TGA and viscosity analysis. Later, its post acceleration effect was explored via AA grafting. Main results reveal that irradiation induced severe starch backbone scission. Cleavage mainly occurred at α -1,4 glycosidic bond and the link of C-1 and C-2 atoms within starch glucose unit. Upon 500 kGy irradiation, number-average molecular mass- Mn -decreased from 2.56 × 10 5 to 1.33 × 10 3 Da, conversing macromolecule to small molecule debris. Certain hydroxyls were oxidized to carbonyls. Molecule chain stacking regularity was destroyed. Amorphization occurred shown a crystallinity reduction close to 34%. Synchronously, color be darken, cracks and micropores were generated, thermal-stability and viscosity decreased. In details, whiteness and viscosity decreased from 91.3 ± 0.1 and 1539 ± 5 to 45.3 ± 1.6 and 0 mPas and the fastest speed thermal-decomposition temperature decreased by 6 °C. It seems a dose of 500 kGy EB irradiation deteriorated starch microstructure and physicochemical property sufficiently. However, it promotes post AA grafting efficiently. Via a dose of 100 kGy EB irradiation and certain level AA grafting a QH 2 O as 687.9 ± 19.5 sample was achieved, showing a 1.16 times elevation compared to sample without pre-irradiation, implying sufficient acceleration effect. These findings promote EB irradiation technology use in starch-based functional material design and starch irradiation damage mechanism, sustaining visible use and academic values. In addition, the prepared grafted product possibly sustains a use application in agriculture field, sustaining potential commercial profit, attractive.
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高影响因子
Materials
·
2025
·Vol. 18
·期 17
·4163
·
IF 3.2
Fei Gao,Peng Xu,Yongqing Zhang,Hui Zhang
To address scaling issues in oilfield water injection, a degradable terpolymer scale inhibitor MA-AA-AMPS (terpolymer)was synthesized via aqueous solution polymerization using maleic anhydride, acrylic acid, and 2-acrylamido-2-methylpropanesulfonic acid as monomers. Characterization confirmed the presence of carboxyl, sulfonic, and amide groups in the copolymer with good thermal stability. Scale inhibition tests showed that at 2% dosage, its scale inhibition efficiency exceeded 80%, remaining above 80% in the pH range of 3–8 and over 50% at 150 °C, with excellent tolerance to high-calcium environments. Biodegradation tests revealed BOD5/COD > 0.3, with a biodegradation rate exceeding 50% in 15 days and reaching 83.4% in 30 days, indicating environmental friendliness. This scale inhibitor effectively solves scaling problems in oilfield water injection systems.
研究领域:
石油工程
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高分子化学
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环境科学
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阻垢技术
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材料科学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Lili Zhao,Jiawei Zhou,Peipei Li,Shu Huang,Xiaohua Zhu,Youyu Zhang,Meiling Liu,Shouzhuo Yao
Human immunoglobulin G (HIgG) plays a significant role in diagnosing infections and monitoring autoimmune diseases. However, the development of sensitive and reliable sensing platforms remains a challenge. Based on the Ag 2 S/SnO 2 composites, a dual-mode sensing platform was developed using multifunctional CoOOH nanoflakes for the sensitive detection of HIgG through photoelectrochemistry (PEC) and colorimetry. In the PEC mode, Ag 2 S QDs boost the PEC activity of SnO 2 NFs and facilitate the subsequent antibody immobilization. Ascorbic acid (AA) captures holes in Ag 2 S/SnO 2 to enhance the photocurrent. CoOOH, acting as a secondary antibody label, forms a sandwich structure with the electrode. The loading of HIgG and CoOOH-Ab2 due to specific recognition of antigen-antibody, leads to the quenching of PEC signal. Various amounts of HIgG will bind with different amounts of CoOOH-Ab2, displaying a linear response within the range of 100 pg/mL∼10 μg/mL, with the detection limit of 2.2 pg/mL. In the colorimetric mode, CoOOH efficiently catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB), and various amount of the sandwich biocomplexes formed with different HIgG levels lead to distinct color changes for visual detection. The linear response range is 500 pg/mL∼9.375 μg/mL with a lower detection limit of 0.29 ng/mL. The two independent modes with different mechanisms, based on the same PEC electrode, can validate each other and provide more scientific and reliable results in response to the targets. Thus, this dual-mode sensing platform for targets-induced signaling changes presents a novel strategy for bioanalysis and detection.
研究领域:
生物化学
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分析化学
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纳米技术
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免疫学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Yun Liu,Meng Xiao,Ningxia Xu,Mengsu Yang,Changqing Yi
This study reports a new ratiometric fluorescence probe (referred to as [email protected] @bCDs) and a smartphone-based sensing system for on-site quantitation of Fe 3+ , ascorbic acid (AA), alkaline phosphatase (ALP) and 2,4-dichlorophenoxyacetic acid (2,4-D). [email protected] @bCDs with well-resolved dual emissions is facilely synthesized by covalent conjugation of red-emissive quantum dots (rQDs), the internal standard, with blue-emissive carbon dots (bCDs), the Fe 3+ -specific fluorescent indicator . 2,4-D can specifically inhibit the enzymatic activity of ALP, leading to suppress the production of AA which can reduce Fe 3+ to Fe 2+ and thereafter trigger the fluorescence response of [email protected] @bCDs. Taking advantages of these highly specific cascade catalytic reactions , ratiometric fluorescence schemes are established for sensing Fe 3+ , AA, ALP and 2,4-D both in aqueous solution and on paper-based microarray . The smartphone-based fluorescence detector integrated with the [email protected] @bCDs-based microarray demonstrated good sensing performance with a wide linear range and low detection limit, and is successfully applied to quantitate 2,4-D in cabbage and tap water samples. The reported method which enables point-of-need quantitation in a sample-in-result-out manner and highlights its features of cost-effectiveness and ease-of-use, is ideally for food safety inspection, environment surveillance, health monitoring at point-of-need, especially in low resource settings.
研究领域:
分析化学
·
环境科学
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食品安全
·
生物技术
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DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Najing Fu,Liteng Li,Kaijing Liu,Chan Kyung Kim,Jun Li,Tao Zhu,Jianheng Li,Baokun Tang
With the development of deep eutectic solvents (DESs), more DES-based functional materials have been explored and applied in various areas. In this work, a novel choline chloride-acrylic acid (ChCl-AA) DES polymer, on a 2D magnetic base, was prepared for the recognition of β-lactoglobulin (β-LG) biomacromolecules in milk and for the inhibition of common bacteria such as Escherichia coli ( E. coli ), Pseudomonas fluorescens ( P. fluorescens ), Staphylococcus aureus ( S. aureus ), and Bacillus subtilis ( B. subtilis ). The ChCl-AA DESs were polymerized on the surface of 2D MoS 2 sheets doped with nano Fe 3 O 4 particles, and the resulting polymer was abbreviated poly(ChCl-AA DES)@Fe 3 O 4 @MoS 2 . The free energy (ΔG=−92) of ChCl-AA DES was calculated using the Gaussian software, the composition and structure of poly(ChCl-AA DES)@Fe 3 O 4 @MoS 2 were characterized by field emission scanning electron microscopy , transmission electron microscopy , etc ., the qualitative and quantitative analyses of β-LG were done by fluorescence spectra , sodium dodecyl sulfate polyacrylamide gel electrophoresis and high performance liquid chromatography, and the bioactivity of bacteria was analyzed by flat colony counting. Based on the present analysis, poly(ChCl-AA DES)@Fe 3 O 4 @MoS 2 specifically recognized β-LG in a good fitting Langmuir isotherm (R 2 = 0.9909) and second-order kinetic model (R 2 = 0.9989) by affinity, and evidently inhibited three bacteria, namely, E. coil (65%), S. aureus (50%), and B. subtilis (54%), effectively reducing the relative colony number. As the poly(ChCl-AA DES)@Fe 3 O 4 @MoS 2 material did not only exhibit specific recognition of biomacromolecules, but also had an antimicrobial effect against common bacteria, it could be an ideal separation media or carrier for biomacromolecules in real samples.
研究领域:
生物化学
·
材料科学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
PHYSICA E-LOW-DIMENSIONAL SYSTEMS & NANOSTRUCTURES
·
2022
·
IF 3.369
Shuai Li,Yuanshu Xiao,Guohua Shan,Xinlei Fan,Mengru Wang,Rui Liu,Lixia Jia
Biomimetic structural colors have demonstrated significant practical promise in eco-friendly coloring. However, the development of novel, simple, and effective methods for the fabrication of photonic crystal structural colors remains an enormous challenge, particularly for flexible substrates . In this work, we prepared structural color coatings with bright, full-color, and low angle dependence on flexible textile substrates via simple vacuum-assisted filtration self-assembly (VAFS). The resultant photonic crystals were polycrystalline structures containing both face-centered cubic (FCC), simple cubic (SC), and amorphous structures (APS), resulting in structural color coatings with bright and low angle dependence. The precise regulation of different hues of structural colors can be achieved by changing the size of microspheres or mixing of microspheres with different sizes. Furthermore, various microspheres (e. g., SiO 2 , SiO 2 @PDA or P (St-MMA-AA)) and fabrics (cotton, linen, silk or wool fabric) can be utilized as self-assembly materials to create structural color coatings, demonstrating the simplicity, low-cost, and great compatibility. Meanwhile, this method may also be applied to the preparation of structural color coatings on substrates with pores, expanding the application of structural colors in various fields.
研究领域:
材料科学
·
纺织工程
·
光学
·
纳米技术
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DOI
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 22
·4555-4566
·
IF 6.331
Wenhui Chen,Qiu Han,Yang Liu,Yiwen Wang,Fu Liu
Hyperphosphatemia, a common complication of chronic renal failure patients, is described as an excess amount of serum phosphate >4.5 mg dL−1. Current therapy for hyperphosphatemia is limited by low removal efficiency, secondary hyperparathyroidism, uremic bone disease, and the promotion of vascular and visceral calcifications. Metal organic frameworks (MOFs) have aroused great interest in the field of blood purification because of their strong specific adsorption. Herein, we prepared mixed matrix microspheres (MMMs) encapsulated NH2-MIL-101(Fe) with specific adsorption to blood phosphate. Simultaneously, a heparinoid copolymer poly (acrylic acid-sodium 4-vinylbenzenssulfonate) (P(AA-SSNa)) was incorporated to improve the hemocompatibility. The proposed MMMs exhibited excellent phosphate adsorption capacity both in aqueous and human plasma environments. They also showed comprehensive hemocompatibility e.g. low tendency of protein adsorption, low hemolysis rate and extended blood coagulation time. In general, we envision that the MMMs are potentially suitable as highly efficient hemoperfusion adsorbents for hyperphosphatemia treatment.
研究领域:
药学
·
肾病学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Ruihong Dong,Mengting Zhu,You Long,Qiang Yu,Chang Li,Jianhua Xie,Yousheng Huang,Yi Chen
This study evaluated chemical compositions of green coffee beans from multi-production regions and correlated this information with thermal contaminants in roasted coffee. Using multivariate statistical techniques, formation of 5-hydroxymethylfurfural (5-HMF), furan, 2- and 3-methylfuran were positively correlated with lipid, sucrose, glutamic acid, phenylalanine, margaric acid, linolenic acid and trigonelline in green coffee beans. Moreover, significant positive correlations between acrylamide (AA) levels with aspartic acid, serine, alanine, histidine, asparagine, protein, and caffeine was found in green beans. Despite this, 5-HMF, furan, 2- and 3-methylfuran showed negative correlations with active constitutes (neochlorogenic acid, cryptochlorogenic acid, caffeine, total phenolics (TPC) and total flavonoids contents (TFC)), and several amino acids, and there were slight negative relationships between AA and myristic acid, palmitic acid, chlorogenic acid, sucrose, lipid, TPC and TFC. This study provides valuable enlightenment for the selection of proper coffee beans for production of coffee with high nutrition and low chemical hazardous risks.
研究领域:
食品科学
·
分析化学
·
营养学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2018
·Vol. 34
·期 6
·1051-1057
·
IF 1.248
Dong Yanying,Yu Ping,Sun Qilong,Lu Yang,Tan Zhenjiang,Yu Xiaopeng
Effective molecularly imprinted membranes(MIMs) were developed as an efficient adsorbent for the selective removal of p -hydroxybenzoic acid( p -HB) from acetylsalicylic acid(ASA, aspirin). The MIMs were grafted successfully from poly(vinylidene fluoride) microfiltration membranes via reversible addition-fragmentation chain transfer(RAFT) polymerization. The graft copolymerization of acrylic acid(AA) in the presence of template p -hydroxybenzoic acid led to molecularly imprinted polymer(MIP) film coated membranes. The obtained MIMs were characterized by scanning electron microscopy(SEM), Fourier transform infrared spectrophotometer(FTIR) and Raman spectra, and batch mode adsorption studies were carried out to investigate the specific adsorption equilibrium, kinetics and selective recognition properties of different MIMs. The kinetic properties of the MIMs could be well described by the pseudo-second-order rate equation. Selective permeation experiments were performed to evaluate the permeation selectivity of the p -HB imprinted membranes. The observed performances of the MIMs are applicable to the further purification of aspirin.
研究领域:
材料科学
·
化学工程
·
环境科学
·
药学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Shi Gang Liu,Lei Han,Na Li,Yu Zhu Fan,Yu Zhu Yang,Nian Bing Li,Hong Qun Luo
The development of ratiometric fluorescent strategy is very significant and challenging in bioanalysis. Herein, graphitic carbon nitride (g-C 3 N 4 ) nanosheets and cobalt oxyhydroxide (CoOOH) nanoflakes are exploited for ratiometric fluorescence assay of alkaline phosphatase (ALP) activity. In the g-C 3 N 4 /CoOOH nanohybrid, g-C 3 N 4 nanosheets serve as a signal unit and the CoOOH nanoflakes function as a recognition element, and initially, the fluorescence of g-C 3 N 4 is quenched by the CoOOH nanoflakes. In the absence of target, the CoOOH nanoflakes of the nanohybrid system are able to oxidize o-phenylenediamine (OPD), and the resultant oxidation product (OxOPD) quenches the blue emission of g-C 3 N 4 and meanwhile emits orange fluorescence which acts as another signal element. However, an efficient redox reaction between ascorbic acid (AA) and CoOOH can cause decomposition of the CoOOH nanoflakes, and additionally, ALP can catalytically hydrolyze L-ascorbic acid-2-phosphate (AAP) to generate AA. Thus, in the presence of target, the CoOOH nanoflakes are destroyed by AA preferentially and the OPD is rarely oxidized to OxOPD, accompanied with strong blue emission of g-C 3 N 4 and weak orange fluorescence from OxOPD. Target-dependent dual-signal change made the ratiometric assay possible, and also AA-induced signal variation was investigated and attributed to the stronger reducing capacity of AA than OPD. The ratiometric sensing platform for ALP activity assay provides a new perspective for the applications of two-dimensional nanomaterials to develop novel and sensitive biosensors.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
生物医学工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）