MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 7
·492
·
IF 5.4
Shen Fei,Wang Sen,Yang Qian,Li Guanglei,Lin Xianchang,Chen Guanyao,Wang Ji,Sheng Rizhi
Endotoxins, specifically lipopolysaccharides (LPS), are components of the outer membrane of Gram-negative bacteria. Due to the fact that even extremely low concentrations of endotoxins can trigger the human body’s inflammatory cascade, there is an urgent need to develop highly sensitive detection technologies for accurate identification and quantitative analysis of trace amounts of endotoxin. This study, based on an electrochemical sensing platform constructed using AuNPs/NH 2 –UiO-66/PEI/MCNs functionalized nanocomposite materials, has successfully achieved efficient detection of endotoxins in food. Given the complex food matrix and numerous interfering substances, this sensor enhances its anti-interference capability through a multi-level cooperative interface design. It uses polyethyleneimine-modified mesoporous carbon nanospheres (PEI/MCNs) as the substrate and integrates the directional loading capability of NH 2 -UIO-66 with the conductivity-enhancing effect of gold nanoparticles (AuNPs), thereby significantly improving signal stability. This biosensor achieves a remarkable detection limit as low as 40 fg/mL, enabling trace-level identification of LPS. Additionally, it exhibits excellent selectivity against common foodborne interferents, facilitating rapid and accurate LPS detection.This study presents a novel, portable detection solution for the rapid screening of microbial contamination in food. The nanocomposite interface construction strategy employed herein provides significant guidance for developing trace-level hazard detection technologies in complex matrices. Graphical
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Yaoxing Yang,Yao Zhao,Yunqi Wang,Ziqiang Yin,Minqiao Feng,Yezi Fan,Tingting Linhu,Jinfeng Li,Dongli Deng,Shuxia Xu
Kanamycin (Kana) residues in water pose significant threats to ecosystems and human health, highlighting the need for rapid and simple on-site monitoring methods. Conventional salt-induced aggregation of gold nanoparticles (AuNPs) for colorimetric detection often suffers from limited sensitivity and poor color contrast. Herein, we report a dual-mechanism enhanced colorimetric biosensor that combines charge neutralization by positively charged Kana with charge shielding by NaCl to amplify AuNPs aggregation. This synergistic effect triggers a distinct color change from wine-red to dark blue within 3 min, enabling rapid visual and quantitative detection of Kana. The aggregation behavior of AuNPs was characterized by UV–Vis spectroscopy, transmission electron microscopy, dynamic light scattering, and Zeta potential measurements. Under optimized conditions, the sensor exhibited a linear range of 5–250 nM with a limit of detection (LOD) of 4.4 nM based on UV–Vis analysis. Smartphone-based RGB analysis achieved instrument-free quantification in the range of 0–250 nM, with an LOD of 2.3 nM. The biosensor also demonstrated excellent selectivity toward Kana against common interfering substances and was successfully applied to spiked environmental water samples, yielding recoveries of 94.69%–105.90% with RSDs below 7.34%. This work provides a rapid, sensitive, and portable platform for on-site monitoring of antibiotic contamination.
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OPTICS AND LASER TECHNOLOGY
·
2026
·
IF 5.2
Like Li,Shunye Huang,Yi Li,Xiaoman Chen,Qiwei Li,Hui Tang,Xuqiang Wu,Yong Zhao
Due to its narrow treatment window, the vancomycin concentration in the patient’s body needs to be monitored regularly to guide dose optimization and ensure safety and efficacy. Here, we developed a fiber-optic surface plasmon resonance (SPR) vancomycin sensor based on gold nanoparticles (AuNPs)/multiwalled carbon nanotubes (MWCNTs)/DNA aptamer synergistic modification. AuNPs can effectively enhance the local electromagnetic field and plasmon resonance effects, while MWCNTs have a strong electronic conductivity and a high specific surface area. The synergistic effect of the two can achieve dual amplification of the SPR signal, thereby improving the sensitivity. Vancomycin aptamer can specifically capture vancomycin molecules and induce spatial conformational changes, thereby increasing the effective refractive index. Experimental results confirmed that within 30 μM–400 μM, the sensitivity of vancomycin detection is as high as 0.0527 nm/μM, and the detection limit is 18.11 μM. Moreover, the vancomycin sensor exhibits excellent stability and specificity. The vancomycin sensor is low-cost, easy to use, and has the potential to be applied for rapid and accurate determination of vancomycin in clinical settings.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Yuwan Du,Yangxuepan Wang,Jinliang Ning,Sifan Cao,Dongmei Yang,Zhenchao Zhang,Yuan Liu,Xincun Dou
Qualitative and semi-quantitative detection and identification of multiple targets is of great importance in complex sample analysis, while surface-enhanced Raman scattering (SERS) could be an efficient strategy. Here, to efficiently promote the enhancement effect, Ni doping and Au nanoparticles (AuNPs) surface modification were adopted to develop a MoS 2 SERS substrate composed of intercrossed nanoflowers, named AuNPs@Ni-MoS 2 , which exhibits high SERS enhancement towards the Raman reporter (4-mercaptobenzoic acid, 4-MBA) with an enhancement factor (EF) of 10 8 . In complex matrices of different mushrooms, this substrate can also sensitively detect α -amanitin and β -amanitin with a limit of detection (LOD) of 10 −8 M for both, which laid a solid foundation for the accurate discrimination via the following machine learning-based approach. Meanwhile, based on the AuNPs@Ni-MoS 2 substrate, sensitive and specific detection of Hg 2+ was attributed to the formation of an Au-Hg alloy, which could greatly lower the catalytic potential required for the oxidation of the colorless chromogenic agent (3,3’,5,5’-tetramethylbenzidine, TMB) to a yellow oxidized TMB (TMB 2+ ). This study not only provides a reference model for improving charge transfer efficiency in SERS system but also offers technical support for rapid screening multiple analytes in complex samples.
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Journal of Materials Chemistry B
·
2025
·
IF 5.7
Siyue Wang,Xiaotian Guan,Shuqing Sun
C-reactive protein (CRP) is a well-established biomarker whose plasma levels increase significantly during inflammatory and infectious episodes. It plays a vital role in the diagnosis of bacterial infections, autoimmune diseases, and cardiovascular risk assessment. However, conventional detection techniques often struggle to balance high sensitivity with a broad dynamic range. Here, we developed and validated a novel fluorescence-based aptasensor named AuCA (AuNP-enhanced CRISPR Aptasensor), which integrates nucleic acid aptamers, magnetic beads (MBs), gold nanoparticles (AuNPs), and the CRISPR/Cas12a system for robust CRP quantification. In this system, MBs functionalized with aptamer 1 selectively captured the target protein, while AuNPs co-modified with aptamer 2 and a Cas12a activation sequence (Trigger) enabled efficient signal transduction. Upon target binding, the Trigger activated the Cas12a/crRNA complex, initiating trans-cleavage of fluorescent reporters and producing a markedly amplified signal. The optimized AuCA platform achieved a low detection limit of 60 ng/mL and a quantifiable range of 0.1 -150 µg/mL. It exhibited excellent specificity and resistance to biological interference, ensuring reliable measurements even in complex sample matrices. When applied to clinical human plasma, AuCA demonstrated strong concordance with results from a commercial immunoturbidimetric assay. AuCA allowed the simultaneous detection of both standard and hypersensitive CRP (hsCRP), supporting comprehensive full-range CRP (frCRP) analysis with strong potential for clinical application.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Xueyu Chang,Yaqian Cheng,Xinke Wang,Yonghui Wang,Xueli Liu,Tie Han,Zhixian Gao,Huanying Zhou
A fluorescent biosensor strategy was developed in combination with immunomagnetic separation for rapid and sensitive detection of staphylococcal enterotoxin B (SEB). Magnetic nanoparticles (MNPs) modified with aptamer of SEB could capture the SEB. Then the gold nanoparticles (AuNPs) fluorescent probe was added and a “sandwich structure” was formed between AuNPs, SEB and MNPs. The MNPs-SEB-AuNPs structure could be separated with an additional magnetic field, which resulted the lower signals of AuNPs fluorescent probe. In optimal conditions, the current method displayed a broad quantitative range from 100 to 10 7 fg/mL and the limit of detection was 3.43 fg/mL. The recovery of SEB-spiked milk samples ranged from 92.00 to 119.00 %, which revealed that the developed method had great accuracy. Furthermore, the method was fast and economical for ultrasensitive detection. Therefore, the fluorescent biosensor based on MNPs-AuNPs is promising for the detection of other environmental and food pollutants.
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IONICS
·
2023
·Vol. 29
·期 4
·1571-1578
·
IF 2.8
Zhang Sheng-Feng,Li Jing,Wei Mei-Jie,Kong Fen-Ying,Zhang Rui,Wang Zhong-Xia,Fang Hai-Lin,Wang Wei
Herein, a fast, inexpensive, and sensitive electrochemical approach for the quantification of 6-gingerol in the ginger samples was established. Au nanoparticles-ZnO-reduced graphene oxide (AuNPs-ZnO-rGO) nanocomposites were fabricated and employed as electrode-modified materials to improve the sensitivity of the determination. UV–visible (UV–vis), transmission electron microscope (TEM), and X-ray photoelectron spectroscopy (XPS) characterization elucidated the successful fabrication of AuNPs-ZnO-rGO nanocomposites. Voltammetry investigation demonstrated the excellent electrocatalytic activity of AuNPs-ZnO-rGO nanocomposites toward the redox reaction of 6-gingerol. Using the best experimental conditions (pH: 2.0 BR; the amount of AuNPs-ZnO-rGO suspension: 6 μL; accumulation time: 120 s), the linearity of the peak currents relied on the concentration of 6-gingerol from 0.1 to 100 μM with a detection limit of 0.03 μM (S/N = 3). The developed approach was successfully implemented for the quantification of 6-gingerol in ginger samples, with recoveries of 99.07‒102.1%.
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Analytical Methods
·
2022
·Vol. 14
·期 20
·1996-2002
·
IF 3.532
Hongwei Zhang,Yuan Qu,Yanchao Zhang,Yumei Yan,Haixiang Gao
Herein, we report the preparation of a gold nanoparticle-thioglycolic acid (TGA@AuNP) suspension for the label-free colorimetric detection of the organophosphorus pesticide chlorpyrifos. This colorimetric sensor was synthesized by a one-pot method, providing thioglycolic acid (TGA)-modified gold nanoparticles with a negatively charged surface. The formation of TGA@AuNPs was confirmed by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and dynamic light scattering (DLS). In addition, chlorpyrifos was quantitatively determined by UV-vis spectrophotometry. The modification time, TGA concentration, pH, incubation time, temperature, ionic strength, and interference of other pesticides were also investigated. The synthesized TGA@AuNP colorimetric sensor possesses a detection limit as low as 20 μg L−1 and a selective sensing response, and the detection time is less than 2 minutes. In addition, this method can also be applied to detect various practical samples in life, such as environmental water samples and tea.
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Advanced Materials Interfaces
·
2021
·Vol. 9
·期 3
·2101461
·
IF 6.147
Qiang Zhang,Zhao Dong,Xiushan Dong,Qianqian Duan,Jianlong Ji,Yan Liu,Zhen Pei,Chao Ji,Shengbo Sang
The membrane based on receptor functionalization provides a new paradigm for the development of mechanical biosensors. However, improvement of sensitivity and test accuracy is still a challenge for mechanical biosensors in practical application. Herein, a surface stress mechanical biosensor (MBioS) based on double-side-gold nanoparticale (AuNP)-coated grid-type polydimethylsiloxane (PDMS) membrane (D-G-MBioS) and 3D printing for human serum albumin (HSA) detection is developed. The surface stress is amplified by the grid coupling sandwich immune structure to improve the sensitivity of the MbioS, successfully reducing limite of detection (LOD) by two orders of magnitude. By self-assembly of AuNPs, the double-side-coated PDMS membrane is prepared, and the detection accuracy of the sensor is improved. The 3D printing is employed to improve the consistency of the MBioS. The D-G-MBioS system is constructed, realizing the HSA detection in range of 0–50 µg mL −1 with a LOD of 20.6 ng mL −1 . In addition, the D-G-MBioS based on sandwich assay is exhibited the high specificity, selectivity, stability, and repeatability. Especially, the D-G-MBioS can detect the HSA in real samples. This study provides a way to improve the sensitivity of mechanical biosensor.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2020
·
IF 3.209
Xu Hun,Xiaohui Kong
Alzheimer’s disease is a worldwide health problem and it has attracted extensive attention. Tau protein is an important biomarker in the pathogenesis of Alzheimer’s disease. Herein, we devise a in situ enzyme catalysis generating electron donor photoelectrochemical (PEC) biosensor for Tau-381 protein. Tau-381 protein aptamer is immobilized onto the surface of AuNPs/MoSe 2 nanosheets modified electrode. In the presence of Tau-381 protein, an aptamer-protein duplex is formed. Meanwhile, the Tau-381 antibody and the protein G/AP (protein G labeled with alkaline phosphatase) are captured with the affinity interaction between Tau-381 protein and Tau-381 antibody, Tau-381 antibody and protein G/AP. The electron donor, ascorbic acid, is in situ produced by the catalyzing of ascorbic acid 2-phosphate in the PEC detection solution. As a result, low blank noise and strong photocurrent response are engendered. The photocurrent response is related to the concentration of Tau-381 protein. The detection range of Tau-381 protein is from 0.5 fM to 1.0 nM with detection limit of 0.3 fM. This in situ generating electron donor PEC biosensor can detect various targets by simply alternating antibody, antigen, or aptamer commercially. Thus, this work represents a simple and general sensing protocol.
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TALANTA
·
2020
·
IF 5.339
Yuhuan Wang,Zhixing Zeng,Jianyu Qiao,Shuqing Dong,Qing Liang,Shijun Shao
An ultrasensitive and high-performance electrochemical nitrite sensing platform based on gold nanoparticles deposited on poly (dimethyl diallyl ammonium chloride)-decorated MXene (Ti 3 C 2 T x ) (AuNPs/Ti 3 C 2 T x -PDDA) was constructed. AuNPs/Ti 3 C 2 T x -PDDA on the surface of electrode displayed synergetic catalytic effect for oxidizing NO 2 ‾ originating from especially catalytic activity of AuNPs, large area and excellent conductivity of Ti 3 C 2 T x , as well as electrostatic interaction of PDDA. The amperometry technique was employed for quantitative determination of nitrite, in which the AuNPs/Ti 3 C 2 T x -PDDA/GCE sensing platform showed outstanding linear relationship in 0.1–2490 μM and 2490–13490 μM for nitrite, meanwhile the detection limit of 0.059 μM. Besides, the prepared sensor possessed high sensitivity of 250 μA mM −1 cm −2 yet excellent selectivity, stability and reproducibility. Furthermore, this platform also exhibited satisfactory feasibility of nitrite sensing in running water and ham sausage sample. This work would broaden a facile approach to construct high sensitivity electrochemical sensing platform via two-dimension materials and its nanocomposites.
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JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 55
·期 16
·7029-7038
·
IF 3.553
Li Jingwen,Wang Wen,Xie Chengbei,Jia Qin,Yan Changchun,Yao Yue,Fang Zheng,Duan Lingfeng,Cheng Jie,Qu Lulu,Han Caiqin
2,3,7,8-Tetrachlorodibenzo- p -dioxin (TCDD) is a highly toxic persistent organic pollutant (POP) that can remain present in the environment for a long time. Prolonged exposure to TCDD may bring about a wide variety of deleterious health effects to humans. Based on these circumstances, a rapid and facile surface-enhanced Raman scattering (SERS) method with gold nanoparticle-modified silver nanorod (AuNP@AgNR) substrates was developed for detection of 2,3,7,8-TCDD. Compared with SERS-active gold nanoparticles and silver nanorod substrate, the prepared AuNP@AgNR substrate showed an outstanding SERS performance with an enhancement factor of up to 2.4 × 10 8 . Furthermore, using AuNP@AgNR as a SERS substrate, we achieved the highly sensitive detection of 2,3,7,8-TCDD, and a density functional theory calculation was 6 to assign the characteristic peaks of 2,3,7,8-TCDD. The reliable linear range was determined to be 10 ng mL −1 to 10 μg mL −1 , with the limit of detection and the limit of quantitation as low as 7.1 and 8.2 ng mL −1 , respectively. By this method, the recovery rates of 2,3,7,8-TCDD spiked in the river samples were 82.3–97.4% with a relative standard deviation between 1.9 and 8.4%. These results show that the AuNP@AgNR substrate can be used for highly sensitive detection of 2,3,7,8-TCDD and has potential applications in the detection of other POPs.
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