ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Ruijing Li,Weili Yang,Xingxue Yan,Xinkui Zhou,Xiaorui Song,Cuihua Liu,Yaodong Zhang,Jitong Li
Bisphenol A (BPA) is a widespread environmental endocrine disruptor (EED) that can cause various environmental and health issues by inducing oxidative stress. The c-Jun N-terminal kinase (JNK) signaling pathway plays a crucial role in oxidative stress-mediated cellular damage. Although folic acid (FA) has demonstrated antioxidant properties, its potential protective effects against BPA-induced developmental and neurotoxicity, as well as the mechanisms involved in the JNK signaling pathway, are still not completely understood. Zebrafish embryos were exposed to different concentrations of BPA ranging from 20 to 40 µM, with or without treatment of 50 µM FA, starting at 6 hours post-fertilization (hpf). Various parameters such as hatchability, survival rate, body length, and heart rate were measured and analyzed. Transcriptome sequencing was conducted to study the changes in gene expression. Oxidative stress markers, including reactive oxygen species (ROS), lipid peroxidation (LPO), hydrogen peroxide (H 2 O 2 ), and catalase (CAT) activity, were assessed. The expression of proteins related to the mitogen-activated protein kinase (MAPK)/JNK pathway was analyzed using western blot. Neurodevelopmental and apoptotic outcomes were evaluated through behavioral tests, immunofluorescence and RT-qPCR examinations. The study found that exposure to BPA led to a decrease in hatchability, survival, body length, heart rate, total antioxidant capacity and promoted apoptosis in zebrafish larvae. However, supplementation with FA was able to alleviate these negative effects. BPA exposure increased levels of ROS, LPO, and H 2 O 2 , while decreasing CAT activity in zebrafish larvae. Treatment with FA effectively reduced BPA-induced oxidative stress and restored antioxidant defense systems. Moreover, KEGG pathway enrichment analysis revealed that the MAPK signaling pathway was the most enriched signaling pathway. Further studies revealed that BPA activated the JNK signaling pathway, while FA suppressed this activation. Additionally, FA significantly improved BPA-induced neurobehavioral deficits and protected against neurocytological alterations. Our findings demonstrate that FA effectively protects against BPA-induced developmental and neurotoxic effects in zebrafish by suppressing oxidative stress and inhibiting the JNK signaling pathway. This study provides new strategies and insights for preventing BPA-induced developmental and neurotoxicity in aquatic organisms.
研究领域:
环境科学
·
毒理学
·
发育生物学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environment & Health
·
2023
·
IF 0
Huiming Cao,Zhunjie Li,Bolei Chen,Jing Wang,Zhen Zhou,Zhi Li,Yun Qian,Yong Liang
Remarkable antibacterial activity of BPA analogues especially for tetrabromobisphenol A against Staphylococcus aureus 25923 (Sa25923) and methicillin-resistant Staphylococcus aureus (MRSA) has been reported in our previous studies. However, the toxic effects of the compounds as environmental contaminants on the endocrine system limited their applications in the field of medicine and health. Given the abuse of antibiotics has led to the emergence of multiple super-resistant bacteria, we considered that structural modifications based on the BPA structure will be available for molecular designing of potential antimicrobial agents without drug resistance. In this study, to further improve the antibacterial activity and reduce the biological toxicity, we performed the computational models to evaluate the binding affinities of BPA analogues to the potential target DltA protein in the biosynthesis of cell wall. A series of synthesized achiral analogues of α,α,α′-tris(4-hydroxyphenyl)-1-ethyl-4-isopropylbenzene (α,α,α′-TEIB) exhibited low minimum inhibitory concentration against Sa25923 and MRSA (2 or 1 μg mL–1). Especially, the analogue A4 did not induce the drug-resistant mutants for all tested Gram-positive bacterial strains and exhibited relatively lower cytotoxicity in HepG2 cells. The developed classification model based on the light gradient boosting algorithm showed the superior performances on the internal robustness and generalization ability for the ligand-based virtual screening of bisphenol and polyphenol antimicrobial substances. Collectively, our findings suggest that the molecular structure of α,α,α′-TEIB is promising as a scaffold, which is expected to achieve a breakthrough in the development of antibiotics which can prevent the invasion of MRSA and other super bacteria.
研究领域:
微生物学
·
药物化学
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计算生物学
·
毒理学
✓ 已验证
DOI
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Xiaojing Guo,Yejing Huang,Wanxiang Yu,Xiao Yu,Xiufen Han,Haiyun Zhai
Multi-walled carbon nanotubes modified with iron oxide and manganese dioxide (MWCNTs-Fe 3 O 4 −MnO 2 ) was synthesized by using iron oxide (magnetic agent) and manganese dioxide (surface enhancer agent) to further approach in a magnetic solid-phase extraction (MSPE) coupled with high performance liquid chromatography (HPLC) method for the determination of bisphenol A (BPA) in plastic bottled drink samples. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray (EDX), Fourier-transform infrared spectroscopy (FTIR), vibrating sample magnetometer (VSM), and BET surface area measurement (BET) were used to characterized the materials. MWCNTs-Fe 3 O 4 MnO 2 are more effective for BPA adsorption than other materials which compared in this study. The binding performances of sorbent were evaluated by static and kinetic adsorption experiments. The data were well fitted to the Langmuir isotherm model and pseudo-second-order model, which the maximum adsorption capacity ( q max ) and the contact time of initial concentrations to achieve equilibrium were found to be 132.9 mg g −1 and 150 min, respectively. After adsorption, MWCNTs-Fe 3 O 4 −MnO 2 can be conveniently and quickly separated from the media by an external magnetic field, and adsorption-desorption capacity can remain at least 6 times of usage. Several parameters affecting the extraction efficiency were investigated. Under optimal conditions, the sorbent was successfully applied to MSPE followed by HPLC determination of BPA in real sample. The limit of detection (LOD) and limit of quantification (LOQ) values of MSPE-HPLC was 0.092 ng mL −1 and 0.167 ng mL −1 , respectively. Spiked recoveries ranged from 94.9 to 103.4% for four real samples, with relative standard deviations of less than 6.7%. It was concluded that the synthesized MWCNTs-Fe 3 O 4 −MnO 2 could be used as an efficient adsorbent for the determination of BPA in different plastic bottled drink samples.
研究领域:
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL RESEARCH
·
2024
·
IF 7.7
Yaxuan Liu,Chen Yu,Huixia Lu,linan Liu,Jingchun Tang
Photocatalytic property of nano Ag is weak and its enhancement is important to enlarge its application. Herein, a novel strategy of constructing silver g-C 3 N 4 biochar composite (Ag-CN@BC) as photocatalyst is developed and its photocatalytic degradation of bisphenol A (BPA) coupled with peroxydisulfate (PDS) oxidation process is characterized. Characterization result showed that silver was evenly embedded into the g-C 3 N 4 structure of the nitrogen atoms format, impeding agglomeration of Ag by distributing stably on biochar. In optimum condition, BPA of 10 mg/L could be degraded completely at pH of 9.0 with a 0.5 g/L photocatalyst, 2 mM PDS in Ag-CN@BC-2 (Ag/melamine molar ratio of 0.5)/PDS system (99.2%, k = 4.601 h −1 ). Ag-CN@BC shows superior mineralization ratio in degrading BPA to CO₂ and H₂O via active radical way, including holes ( h ⁺), superoxide radicals (•O 2 ⁻ ), sulfate radicals (SO 4 • ⁻ ), and hydroxyl radicals (•OH). Proper amount of silver can be dispersed effectively by gC 3 N 4 , which is responsible for improving the visible-light absorbing capability and accelerate charge transfer during activation of PDS for BPA degradation, while biochar as carrier in the composite is supposed to enhance the photoelectric degradation of BPA by reducing the band gap and increasing the photocurrent of Ag-CN@BC catalyst. Ag-CN@BC exhibits excellent catalyst stability and photocatalytic activity for treatment of toxic organic contaminants in the environment.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Yiyun Zhang,Wenwen Zhang,Liming Zhang,Guangsan Song,Ningwei Wang,Wanzhen Xu,Weihong Huang
An imprinted electrochemical sensor based on multifunctional multi-walled carbon nanotubes was synthesized for detection of bisphenol A (BPA). Molecularly imprinted polymers (MIPs) consist carboxylated quantum dots grafted with aminated multi-walled carbon nanotubes as a carrier, BPA as a template, and 3-aminopropyltriethoxysilane (APTES) as a monomer. The successful recombination of quantum dots allows the electrodes to translate small differences in concentration into large changes in current response. Modified materials were discussed by TEM, SEM, FT-IR, Fluorescence, XRD, together with a series of characterization of electrochemical behavior. The MIPs displayed well selectivity towards BPA and hardly showed response to interferes. Also, the sensor has a high stability (RSD of 5.17%) and repeatability (RSD of 0.43%). Moreover, the current response has a linear relationship range from 0.05 to 50 nmol L −1 , with a detection limit of 0.015 nmol L −1 . Finally, the modified electrode was utilized for the detection of 3 kinds of water samples which indicated that the sensor has potential for future applications in trace detection of BPA in environment water.
研究领域:
环境科学
·
材料科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Ye Yang,Fangbo Zhao,Liu Yang,Jiaming Zhang,Hee-Deung Park,Zhiguo Li,Hongxu Chen,Ximeng Zhang,Mingjin Gao
Most ultrafiltration (UF) membranes can hardly remove organic pollutants with low molecular weight, like Bisphenol A (BPA). This study effectively addressed the issue by immobilizing the GO-Ag nano-sheet catalyst within the finger-like pores of a polymer UF membrane using bottom cross-flow filtration. The catalytic membrane operated under a low pressure of 0.2 MPa and achieved a remarkable water flux of 368 L/(m 2 ·h). By filtering aqueous substrates with BPA and humic acid (HA), the UF membrane could effectively reject most of the high molecular weight HA (>90.8%). The BPA entered the membrane pores could be effectively decomposed (>99.4%) by the free radicals generated by the peroxydisulfate(PDS)/GO-Ag system. Also, the catalytic membrane exhibits significantly enhanced BPA degradation kinetics, up to 19.6 times higher than those of conventional suspension-based methods. Moreover, the GO-Ag catalytic membrane demonstrates excellent reusability, maintaining almost constant flux after multiple cycles with a flux recovery rate (FRR) of 90.1%. This is attributed to the in-situ degradation of pollutants attached to the membrane by the free radicals. In addition, it is proved that the GO-Ag catalytic membranes show significant antibacterial abilities. In summary, the GO-Ag/PES catalytic membrane offered significant advantages, such as a convenient fabrication process and the integration of membrane separation and advanced oxidation processes (AOPs) in a single step, eliminating pollutants quickly, saving catalysts and oxidants, and minimizing membrane fouling.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
·
2018
·
IF 4.427
Zhiyuan Meng,Dezhen Wang,Sen Yan,Ruisheng Li,Jin Yan,Miaomiao Teng,Zhiqiang Zhou,Wentao Zhu
The widespread application of bisphenols (BPs) makes them ubiquitous in the natural environment and poses many potential risks. In this study, we examined the effects of perinatal exposure to BPA and its 3 alternatives (BPS, BPF, and BPAF) on lipid and glucose homeostasis in female mice adolescent offspring . Specifically, BPA exposure promoted the expression of hepatic lipid synthesis and fatty acid accumulation genes, resulting in a significant increase in 2 free fatty acids contents. BPS exposure caused an increase in 6 free fatty acids and triglyceride contents through promoting the expression of fatty acid synthesis, triglyceride synthesis and fatty acid accumulation genes and inhibiting the expression of fatty acid β-oxidation genes. Interestingly, BPAF exposure showed completely opposite effects on hepatic lipid metabolism compared to BPS exposure. 9 free fatty acids and triglycerides contents in the liver were significantly reduced. In particular, BPF exposure caused decreases in 2 free fatty acids contents, but no significant changes were found in the genes for lipid metabolism. In addition, unlike BPA and BPF exposure, BPS and BPAF exposure also resulted in significant increases in glucose and glycogen contents in the liver by activation of Fxr-Sh p pathway and glycolysis, and inhibition of gluconeogenesis. The results showed that compared to BPA and BPF exposure, BPS and BPAF exposure significantly regulated the expression of genes related to glucose and lipid metabolism and severely interfered with hepatic lipid and glucose homeostasis. This suggested that we should thoroughly evaluate the potential health risks of BPA and its alternatives.
研究领域:
环境科学
·
分子生物学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
REPRODUCTIVE TOXICOLOGY
·
2024
·
IF 3.3
Chenyan Jiang,Wenke Dong,Guanglin Gao,Wen Sun,Yonghong Wang,Bowen Zhan,Yanyan Sun,Jian Yu
As the incidence of precocious puberty has risen in recent years and the age at puberty onset is younger, children may be at increased risk for health consequences associated with the early onset of puberty. Bisphenol A (BPA) is recognized as an endocrine disruptor chemical that is reported to induce precocious puberty. The effect of BPA exposure modes, times, and doses (especially low dose) were controversial. In the present study, we evaluated the potential effects of maternal exposure to low-dose BPA on the hypothalamus, particularly on the arcuate (ARC) nucleus and anteroventral periventricular (AVPV) nucleus during peri-puberty in offspring of BPA-treated rats. Pregnant rats were exposed to corn oil vehicle, 0.05 mg·kg −1 ·day −1 BPA, or 5 mg·kg −1 ·day −1 from gestation day 1 (GD1) to postnatal day 21 (PND21) by daily gavage. Body weight (BW), vaginal opening (VO), ovarian follicular luteinization, and relevant hormone concentrations were measured; hypothalamic Kiss1 and GnRH1 levels by western immunoblot analysis were also assessed as indices of puberty onset. During or after exposure, low-dose BPA restricted BW after birth (at PND1 and PND5), and subsequently accelerated puberty onset by promoting the expression of prepubertal Kiss1 and GnRH1 in the AVPV nucleus on PND30, leading to advanced VO, an elevation in LH and FSH concentrations (on PND30). We also noted increased BW on PND30 and PND35. Maternal oral exposure to low-dose BPA altered the BW curve during the neonatal and peripubertal periods, and subsequently accelerated puberty onset by promoting prepubertal Kiss1 expression in the AVPV nucleus.
研究领域:
内分泌学
·
生殖生物学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2022
·
IF 8.431
Qi Han,Mingming Wang,Feiyun Sun,Boping Yu,Zijun Dong,Pu Li,Jingwen Luo,Mu Li,Xingliang Jin,Zhiguang Dai
Bisphenol A (BPA), one of the widely known endocrine-disrupting chemicals, can be effectively degraded by advanced oxidation processes in water because of the powerful reactive oxygen species . In this study, Fenton , UV/Fenton, and metal ion/peroxymonosulfate (PMS) processes were compared to investigate BPA degradation efficiency and pathways initiated by hydroxyl radicals and sulfate radicals. In contrast to the Fenton system, which only degraded 60% of BPA within 15 min, the UV/Fenton system could degrade greater than 80% of BPA, because more hydroxyl radicals (•OH) were generated under the reduction of Fe 3+ to Fe 2+ . The optimized parameters of the UV/Fenton system were as follows: 8 μmol/L of Fe 2+ , 80 μmol/L of H 2 O 2 , and a pH value of 3.0. As for the metal ion/PMS system, the BPA degradation efficiency was closely associated with the applied metal ions, and the order was as follows: Co 2+ /PMS (∼100%) > Fe 2+ /PMS (∼80%) > Cu 2+ /PMS (∼79%). The degradation pathways of BPA were theoretically interpreted through density functional theory prediction and degradation products during various processes. Two major initial reaction sites (4C and 6C) for •OH initiated using the UV/Fenton system and one initial reaction site (4C) for sulfate radicals (SO 4 •− ) using the metal ion/PMS system were recognized for BPA degradation processes. The degradation products by •OH showed a larger average molecular weight than those by SO 4 •− . These studies are instructive for the application of different advanced oxidation systems in the treatment process of BPA in wastewater.
研究领域:
环境科学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 12
·5186-5192
·
IF 1.821
Nuo Duan,Hui Zhang,Yu Nie,Shijia Wu,Tingting Miao,Jie Chen,Zhouping Wang
We report a method for trace detection of bisphenol A (BPA) based on fluorescence resonance energy transfer (FRET) between lanthanide-doped KGdF4 nanoparticles (KGdF4:Tb3+ NPs) as a donor, and gold nanoparticles (AuNPs) that act as an acceptor. The KGdF4:Tb3+ NPs were modified with an aptamer recognizing BPA, and AuNPs were modified with the complementary DNA (cDNA) of the aptamer. The donor–acceptor pair was fabricated by hybridizing the aptamers and their cDNA. Thus, fluorescence of the KGdF4:Tb3+ NPs could be quenched due to the good overlap between the KGdF4:Tb3+ NP fluorescence emission and the AuNP absorption spectrum. However, on addition of BPA, the aptamers preferentially bound to BPA, forming KGdF4:Tb3+ NP–aptamer–BPA complexes. As a result, the FRET was disrupted, and the fluorescence was restored. The recovered fluorescence of the KGdF4:Tb3+ NPs was linearly proportional to the concentration of BPA in the range of 0.5 to 100 ng mL−1, with detection limits as low as 0.16 ng mL−1. The assay was applied to real samples, and the results were consistent with the results obtained using high performance liquid chromatography (HPLC) methods. We presume that this strategy can be extended to the detection of other contaminants by simply substituting the aptamer.
研究领域:
分析化学
·
环境科学
·
纳米技术
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Jiayin Liu,Jialing Tan,Hao Liu,Yunjie Yin,Chaoxia Wang
Linen, an important industrial crop, is commonly used in the textile field. The development of temperature-controlled linen fabrics is important for the high-value utilization of cellulose-based industrial products. The linen fabrics with color and performance changes controlled by temperature are prepared via the vacuum impregnation process utilizing biomass three-component thermochromic dyes. Currently, three-component thermochromic dyes based on the bisphenol A (BPA) color developer have received special attention due to high saturation color and reversible color-changing performance. However, the toxicity and carcinogenicity of BPA limit its applications in security inks, decorative coatings, plastics, etc. Exploiting excellent and safe biomass color developers for three-component thermochromic dyes remains a challenge. Tea polyphenols (TP) are proposed as alternatives to BPA to prepare the blue, red, yellow, and green three-component thermochromic dyes. According to the laws of subtractive color mixing, multicolor-tunable thermochromic dyes are obtained by color matching of red, yellow, and blue dyes to span multiple shades. Biomass thermochromic dyes are adopted to fabricate temperature-controlled linen fabrics by the vacuum impregnation process. Temperature-controlled linen fabrics can change color reversibly between the original color and achromatic color. The color-changing occurs due to the ring opening and closing of leuco dyes triggered by the solid-to-liquid transition of solvent. The phase transition temperature of the thermochromic dyes absorbed on the linen fabric is 46 ℃. As the temperature increases, the contact angle of temperature-controlled linen fabrics decreases from 129° to 29°, resulting in a change from hydrophobic to hydrophilic with 49 J g −1 of thermal energy stored. The linen fabrics exhibit temperature-controlled changes in color, surface wettability, strength, and thermal insulation. This eco-friendly method with biomass thermochromic dyes for the preparation of color-changing linen fabrics provides a new approach, which is contributing to the development of cellulose-based smart color-changing textiles.
研究领域:
纺织科学
·
材料科学
·
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
·
2016
·Vol. 15
·期 8
·1046-1053
·
IF 2.235
Zhang Guangshan,Wang Qiao,Zhang Wen,Li Tian,Yuan Yixing,Wang Peng
This work investigated the effects of organic acids and initial solution pH on the photodegradation of BPA in a photo-Fenton-like process using α-FeOOH as a catalyst. The results showed that the addition of different organic acids affected the formation of the ferric-carboxylate complexes and free radicals, which in turn varied the photodegradation efficacy. Compared with the other acids, oxalic acid (OA) was found to be the most effective in enhancing the photodegradation of BPA, which strongly depends on the OA concentration. Particularly, the addition of OA could significantly extend the working pH from an acidic to a neutral range for the photocatalytic process and thus the acidification pretreatment may not be needed. A high photocatalytic degradation of BPA occurred at pH 6.0, due to the formation of ferric-oxalate complexes and ˙OH radicals in the synergistic interactions of OA and α-FeOOH. This finding highlights that the oxalate-promoted photo-Fenton-like process using the α-FeOOH catalyst may be used for wastewater treatment without pH adjustment.
研究领域:
环境科学
·
化学工程
✓ 已验证
DOI
PubMed
高影响因子
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