JOURNAL OF APPLIED POLYMER SCIENCE
·
2017
·Vol. 134
·期 18
·
IF 1.86
Binshuang Wu,Xiaoqing Zeng,Linbo Wu,Bo-Geng Li
In order to obtain high performance bioplastics and thermoplastic elastomers, P(LLA-mb-BSA) multi-block copolymers containing 30 wt % and 70 wt % soft segment (PLBSA30 and PLBSA70 for short) were synthesized via chain extension and coupling of poly(L-lactic acid) (PLLA) and poly(butylene succinate -co- adipate) (PBSA) prepolymers in the presence of 1% nucleating agent (NA). They were characterized with proton nuclear magnetic resonance ( 1 H NMR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), polarizing optical microscopy (POM), thermal gravimetric analysis (TGA), transmission electron microscope (TEM), and tensile and impact test. The relatively high molecular weights of ( M n 8200 g/mol and 3100 g/mol for PLLA and PBSA, respectively) the polyester sequences are helpful to improve the PLLA crystallization and the flexibility of the PBSA soft segment. Nucleation effect further accelerates the crystallization of PLLA segment. The synergistic effect of these factors results in good balance between stiffness and toughness. PLBSA30s containing 1% zinc citrate (ZnCC) or talc behave as ultra-tough bioplastics with ultrahigh impact strength over 50 kJ/m 2 and excellent tensile properties. PLBSA70 containing 1% ZnCC possesses elongation at break of 600% and retains high modulus (56 MPa) and strength (16 MPa) and therefore behaves as an excellent thermoplastic elastomer. The excellent and balanced properties would extend the end applications of these PLLA-based materials. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 44777.
研究领域:
材料科学
·
高分子化学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2014
·
IF 5.9
Yanyu Huang,Yi Luo,Wenjie Zheng,Tianfeng Chen
Radiotherapy displays curative potential for cervical cancer management, but radioresistance occurs during long-term therapy. To overcome this limitation, tumor-targeted nanotechnology has been proposed to enhance the radiosensitivity of solid tumors. Herein, we used biocompatible bovine serum albumin nanoparticles (BSANPs) as carriers of organic selenocompound (PSeD) with folate (FA) as the targeting ligand to fabricate a cancer-targeted nanosystem. The combination of PSeD and BSANPs endowed the nanosystem with higher light absorption and reactive oxygen species (ROS) generation owing to their properties of surface plasmon resonance (SPR) effect, heavy metal effect, high refractive index and nanoparticulate interfacial effect. The combined treatment drastically increased the ROS overproduction, VEGF/VEGFR2 inactivation and inhibition of XRCC-1-mediated repair of DNA damage, thus triggering G2/M phase arrest and apoptosis. Taken together, our findings demonstrate the utility of FA-BSANPs as a promising radiosensitizer to improve cancer radiotherapy.
研究领域:
肿瘤学
·
放射生物学
·
纳米技术
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Caifeng Ding,Yujuan Xu,Yanan Zhao,Hua Zhong,Xiliang Luo
Drug delivery which can offer efficient and localized drug transportation together with imaging capabilities is highly demanded in the development of cancer theranostic approaches. Herein, we report the construction of bovine serum albumin (BSA) gold nanoclusters (BSA@AuNCs) for cell fluoresce imaging and target drug delivery. BSA@AuNCs were modified with cyclic arginine-glycine-aspartate with the product RGD–BSA@AuNCs to enhance cell internalization of the nanoclusters. Furthermore, doxorubicin hydrochloride or doxorubicin (DOX), a widely used chemotherapy drug, was also used to modify RGD–BSA@AuNCs. The final design of the DOX/RGD–BSA@AuNC system was constructed through the disulfide bond. The physical microstructure and biological characterization of the BSA@AuNCs were realized through high-resolution transmission electron microscopy and confocal laser fluorescence microscopy. As the disulfide bonds were cleaved by glutathione in cancer cells, DOX-SH molecules were released from the nanosystem to inhibit the growth of cancer cells. The as-prepared DOX/RGD–BSA@AuNC system can be used not only to deliver drug but also to achieve the antitumor effect by in vivo imaging, demonstrating its promising applications in cancer treatment.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2022
·Vol. 26
·期 3
·821-830
·
IF 2.747
Yang Shaoming,Deng Kaiqiang,Shao Shan,Zhang Jian,Peng Jiaxi,Fang Zhili,Xu Wenyuan
A molecularly imprinted photoelectrochemical (PEC) cathodic sensor was developed for the detection of bovine serum albumin (BSA). The PEC sensor was constructed with NiCo-layered double hydroxides (NiCo-LDHs)/Cu 2 O composite as the visible light active species and molecularly imprinted polymers (MIP) made of the electropolymerization of 4-aminobenzenethiol as the recognition element. Scanning electron microscopy (SEM), electron dispersive spectroscopy (EDS), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) were employed for the characterization of the morphological structure, crystal phase, and composition of NiCo-LDHs/Cu 2 O. Electrochemical method and photoelectrochemical detection technique were used for the study of the sensor. Under the optimal conditions, the photocurrent responses of sensor were linear with BSA concentrations over the range of 2.5 × 10 −10 ~ 1.4 × 10 −8 g/L with a detection limit of 1.9 × 10 −10 g/L ( S / N = 3). This PEC sensing strategy exhibited an effective method for BSA determination with high selectivity and good stability.
研究领域:
分析化学
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光化学
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生物传感器
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2022
·Vol. 33
·期 9
·6576-6591
·
IF 2.779
Wu Jinxiu,Wang Xin,Jia Huiling,Liu Zhaogang,Niu Xiaochao
Fluorescent nano-probes have great potential applications for diagnostics and therapy. In this work, the microwave-assisted Stöber method was used for the surface modification of the spherical nano-phosphor YPO 4 :2%Sm 3+ to solve the problems of hydrophilicity and biocompatibility of spherical fluorescent nano-material YPO 4 :Sm 3+ . Their structural, morphology and optical properties were determined using X-ray diffraction, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectra, TG–DTA curve and fluorescence spectrophotometry. And the adsorption of YPO 4 :2%Sm 3+ @SiO 2 to bovine serum albumin was studied by the static isothermal adsorption method. Results indicated that YPO 4 :2%Sm 3+ @SiO 2 was uniformly monodisperse core–shell nano-sphere with an average diameter of 60–90 nm. The crystal structure and morphology of the phosphor YPO 4 :Sm 3+ was not changed after the surface modification, the thickness of the coating layer was approximately 10–20 nm and the fluorescence intensity and lifetime of the modified product were reduced. The adsorption of YPO 4 :2%Sm 3+ @SiO 2 to protein was the mono-molecular layer adsorption, the adsorption formula was Y = 0.0051 X −0.0000037 and the maximum adsorption amount q = 198.89 mg/g. This study successfully solved the problems of hydrophilicity and biocompatibility of spherical nano-phosphor YPO 4 :2%Sm 3+ .
研究领域:
材料科学
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生物医学工程
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产品关联
DOI
高影响因子
BIOMACROMOLECULES
·
2023
·
IF 6.2
Xiaokang Na,Bowen Zou,Xiaohan Zheng,Ming Du,Beiwei Zhu,Chao Wu
Innovation in green, convenient, and sustainable antimicrobial packaging materials for food is an inevitable trend to address global food waste challenges caused by microbial contamination. In this study, we developed a biogenic, hydrophobic, and antimicrobial protein network coating for food packaging. Experimental results show that disulfide bond breakage can induce the self-assembly of bovine albumin (BSA) into protein networks driven by hydrophobic interactions, and chitosan oligosaccharide (COS) with antimicrobial activity can be stably bound in this network by electrostatic interactions. The inherent antimicrobial activity of COS and the numerous hydrophobic regions on the surface of the BSA-network give the BSA@COS-network significant in vitro antimicrobial ability. More importantly, the BSA@COS-network coating can prolong the onset of spoilage of strawberries in various packaging materials by nearly 3-fold in storage. This study shows how surface functionalization via protein self-assembly is integrated with the biological functioning of natural antibacterial activity for advanced food packaging applications.
研究领域:
材料科学
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食品科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
Optoelectronics Letters
·
2024
·Vol. 20
·期 12
·741-748
·
IF 0.8
Zulpiye Hasanjan,Dou Xiaozong,Zhang Hongyan
In this study, a water-soluble fluorescent probe based on copper nanoparticles (CuNPs) was synthesized using a hydrothermal method with bovine serum albumin (BSA) (BSA@CuNPs) as a protectant, and it was utilized to efficiently detect the concentration of tetracycline (TC). Upon the addition of TC to the BSA@CuNPs probe, its fluorescence intensity is obviously diminished due to the keto-enol functions C=O group in TC connects with the hydroxyl group on the BSA@CuNPs probe to form a non-fluorescent complex, which can be successfully utilized for detecting TC concentration. Linear range and detection limit for TC detection are 0.1–130 µM and 90 nM. Furthermore, the BSA@CuNPs probe was applied to detect TC concentrations in real meat samples, with recoveries ranging from 95.40% to 102.13%, and the relative standard deviation ( RSD ) was below 2.73%. Therefore, BSA@CuNPs probe exhibits high sensitivity and selectivity for TC, which will be used for the study of TC analysis in meat.
研究领域:
分析化学
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食品科学
·
纳米技术
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 26
·3256-3262
·
IF 2.073
Pan Li,Jiawei Li,Minghong Bian,Danqun Huo,Changjun Hou,Ping Yang,Suyi Zhang,Caihong Shen,Mei Yang
In this paper, we developed a label-free fluorescence assay employing BSA-Au NCs, S2O32−, and 2-ME for the highly selective and sensitive detection of Pb2+ in sorghum, river water and tap water. When the BSA-Au NCs reacted with S2O32− in solution, Au(S2O3)23− complexes were formed immediately on the surfaces of BSA-Au NCs. After adding Pb2+ and 2-ME, the BSA-Au NCs rapidly dissolved to form Au–S complexes. As a result, the fluorescence intensity decreased dramatically, allowing quantification of Pb2+ in the aqueous solution. This reaction is highly specific under optimal detection conditions. A good linear relationship between the fluorescence intensity and the concentrations of Pb2+ could be obtained in the range of 10 to 1000 nM (R2 = 0.9910), with a detection limit (LOD; S/N ratio = 3) down to 1.043 nM. Significantly, compared with other studies, we greatly shortened the reaction time by substantial improvements in materials. On the basis of the above-mentioned work, we successfully immobilized S2O32− modified BSA-Au NCs on wax-printed papers to achieve the purpose of simple and real-time field monitoring of Pb2+. We picked up R-values (the red value of the RGB color model) from captured flurescence images under UV light (365 nm) and acquired a linear correlation between R-values and Pb2+ concentrations. This linear correlation exhibits the great potential of this approach. The proposed probe also showed satisfactory selectivity as well as excellent reproducibility. Multiple real sample experiments showed the tremendous prospect of this method, as well.
研究领域:
分析化学
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环境科学
·
食品科学
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DOI
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 4
·2248-2255
·
IF 3.9
Mingze Li,Yundong Zhang,Jinli Ma,Jianshi Du
The lymphatic system is a complex network of lymphatic vessels, lymph nodes, and lymphoid organs. The current understanding of the basic mechanism and framework of the lymphatic system is relatively limited and not ideal for exploring the function of the lymphatic system, diagnosing lymphatic system diseases, and controlling tumor metastasis. Imaging modalities for evaluating lymphatic system diseases mainly include lymphatic angiography, reactive dye lymphatic angiography, radionuclide lymphatic angiography, computed tomography, and ultrasonography. However, these are insufficient for clinical diagnosis. Some novel imaging methods, such as magnetic resonance imaging, positron emission computed tomography, single-photon emission computed tomography, contrast-enhanced ultrasonography, and near-infrared imaging with agents such as cyanine dyes, can reveal lymphatic system information more accurately and in detail. We fabricated an albumin-based fluorescent probe for dual-modality imaging of the lymphatic system. A near-infrared cyanine dye, IR-780, was absorbed into bovine serum albumin (BSA), which was covalently linked to a molecule of diethylenetriaminepentaacetic acid to chelate gadolinium Gd3+. The fabricated IR-780@BSA@Gd3+ nanocomposite demonstrates strong fluorescence and high near-infrared absorption and can be used as a T1 contrast agent for magnetic resonance imaging. In vivo dual-modality fluorescence and magnetic resonance imaging showed that IR-780@BSA@Gd3+ rapidly returned to the heart through the lymphatic circulation after it was injected into the toe webs of mice, facilitating good lymphatic imaging. The successful fabrication of the new IR-780@BSA@Gd3+ nanocomposite will facilitate the study of the mechanism and morphological structure of the lymphatic system.
研究领域:
医学影像学
·
生物医学工程
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DOI
PubMed
高影响因子
Journal of Bioresources and Bioproducts
·
2023
·
IF 0
Yejun Deng,Xiang Wang,Caihong Zhang,Pujun Xie,Lixin Huang
Non-enzymatic glycation can cause the formation and accumulation of advanced glycation end products (AGEs), and it poses great threats to human health. It is urgent to search for safe and efficient inhibitors to prevent reducing sugar induced protein glycation. In this study, we investigated the anti-glycation activity and mechanism of an identified peptide, Asparagine-Tyrosine-Arginine-Arginine-Glutamic acid (NYRRE) from Chinese quince seed protein hydrolysate, by multispectroscopy, confocal imaging, and computational molecular simulation. Firstly, it was found that NYRRE could scavenge hydroxyl radicals and chelate Fe 2+ . Besides, the NYRRE was effective in every stage of fructose induced bovine serum albumin (BSA) glycation. The NYRRE could reduce the formation of fructosamine, carbonyl compounds, glycoxidation products and β -amyloid structure. Meanwhile, NYRRE could protect thiol groups and stabilize the spatial conformation of BSA. The NYRRE presented strong inhibition in fluorescent AGEs, and 68.19% of total AGEs formation was prevented with NYRRE at 15 mmol/L. The results of molecular simulation indicated that NYRRE could insert into the hydrophobic pocket of BSA and interact with hot spots, including arginine and lysine residues. The mechanism of NYRRE inhibiting protein glycation could be due to its antioxidant activity, BSA structure stabilizing ability, and specific bond with glycation sites of BSA. These results provided a valuable reference for developing NYRRE as an efficient antiglycation agent in preventing glycation-mediated diseases.
研究领域:
食品科学
·
生物化学
·
分子生物学
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DOI
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2012
·
IF 3.35
Tianyi Wang,Haitao Jiang,Qinfu Zhao,Siling Wang,Meijuan Zou,Gang Cheng
Three different kinds of silica (S2, S1 and SBA-15) with different particle sizes (130, 430 nm and 1–2 μm) and different pore characteristics (i.e. pore size and shape) were developed as oral vaccine immunological adjuvants and the relationship between the silica architecture and immunological properties was investigated. The silica particles were characterized using SEM, TEM and nitrogen adsorption. Model antigen bovine serum albumin (BSA) was successfully entrapped into the silica pores to produce a sustained release vaccine delivery system. Compared with the responsiveness induced by parenteral administration of BSA emulsified in Freund's complete adjuvant (FCA), oral immunization with the silica/BSA formulation produced a stimulated humoral and mucosal (sIgA) response. The IgG and IgA titers induced by loading BSA was as follows: S1 > S2 > SBA-15. The highest IgG and IgA titers of S1 were attributed to its large honeycombed pores and the optimal particle diameter of 430 nm. The corresponding IgG1 and IgG2a titers were also investigated to confirm that BSA loaded in nanoparticles by oral immunization can induce both T-helper 1- and T-helper 2- (Th1 or Th2) mediated responses. We believe that the results of our research will open up new avenues for the formulation of oral vaccines.
研究领域:
免疫学
·
纳米医学
·
疫苗学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
·
IF 5.6
Huirong Tan,Mingrun Tang,Yufeng Che,Xuanao Li,Zhiwen Tu,Xinghong Zhao,Hongping Wan
In mucosal immunity, targeting microfolds (M)cells of the mucosal epithelium, in combination with suitable adjuvants, is critical for eliciting robust immune response. In the present study, we devised a virus like nanoparticles (VLP)-based nano-delivery platform functionalized with the M cell-targeting peptide iRGD (RGDKGPDC) to enhance the immune responses following intranasal administration. Bovine serum albumin (BSA) was selected as a model antigen, and both BSA and iRGD were conjugated to the VLP surface, forming VLP-BSA-iRGD nanoparticles. Leveraging its M cell-targeting capability, VLP-BSA-iRGD demonstrated enhanced uptake by dendritic cells (DCs) and improved trafficking to lymph nodes compared to BSA alone in a mouse model. VLP-BSA-iRGD enhanced the CCL20 secretion and stimulated the DC activation, as evidenced by an in vitro cell co-culture model. Additionally, it enhanced antigen permeability by disrupting the distribution of the ZO-1 protein in epithelial cells. Importantly, VLP-BSA-iRGD induced stronger humoral and cellular immune responses, accompanied by the upregulation of TLR2/4 expression and activation of the MYD88/NF-κB signaling pathway in DCs. While BSA served as a proof-of-concept antigen, this platform may be broadly applicable to other protein-based subunit vaccines requiring intranasal administration.
研究领域:
免疫学
·
纳米医学
·
疫苗学
·
药物递送
·
黏膜免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）