BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Yao Lu,Hong Ke,Yang Wang,Yao Zhang,Huan Li,Chusen Huang,Nengqin Jia
A ratiometric electrochemiluminescence resonance energy transfer (ECL-RET) platform depended on novel dye BODIPY derivatives was proposed for rapid detection of lactoferrin . This ECL-RET platform is composed of aptamer decorated BODIPY composites and C 60 @BSA, in which BODIPY derivative is the ECL probe and can generate significant resonance energy transfer with K 2 S 2 O 8 . BODIPY derivative and K 2 S 2 O 8 are used as built-in reference signal and calibration signal respectively to eliminate background signal and abnormal change signal by double signal self-calibration process. At the same time, C 60 , as the accelerator of K 2 S 2 O 8 , can effectively increase the ECL signal and further transfer as much energy as possible to BODIPY derivative. Under optimal conditions, the constructed ECL-RET platform exhibited sensitive detection of lactoferrin in the wide linear range of 10 -4 - 850 ng/mL with a LOD of 42 fg/mL. Meanwhile, the proposed ECL-RET aptasensor demonstrated superior stability, specificity and reproducibility, displaying favorable application value in practical diagnosis of this method.
研究领域:
分析化学
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生物化学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Peng Zhou,Xiaowei Huo,Jing Zhang,Yang Liu,Feng Cheng,Xin Cheng,Yunqi Wang,Yongli Zhang
Hydroxylated fullerene (fullerenol) has gained increasing attention in the field of water environment remediation based on its intrinsic properties of photosensitivity and high water solubility. In this work, C 60 fullerenol was added to the Fe(III)/PMS process to enhance diethyl phthalate (DEP) degradation both with and without visible light. The DEP removal ratio was increased from 2.2% to 58.8% with the addition of fullerenol at 60 min in dark condition, and the removal of DEP was completely achieved within 50 min under visible light irradiation. Mechanism investigation shows that carboxyl and carbonyl were formed via keto-enol isomerization at acid condition, resulting in the formation of open-cage fullerenol. The open-cage fullerenol can act as complex agent and electron donor to enhance oxidizing capability of the Fe(III)/PMS process by promoting Fe(III)/Fe(II) cycles. Competitive radical capture tests by electron spin resonance (ESR) and radical quenching analysis demonstrate that hydroxyl radical and sulfate radical were primarily responsible for DEP removal, but not superoxide radical and singlet oxygen. The DEP removal in the F/Fe(III)/PMS process is more efficiency at initial pH ranging from 2.1 to 4.9. Moreover, the DEP degradation route was proposed involving the generation of various intermediary products. This study provides a novel method for the application of fullerene derivatives on environment remediation by combining with metal catalyzed advanced oxidation processes.
研究领域:
环境科学
·
化学
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产品关联
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2016
·
IF 2.243
Rong Tian,Xiaojun Chen,Qingwen Li,Cheng Yao
This work reports a novel signal amplification strategy based on three-dimensional ordered macroporous C 60 -poly(3,4-ethylenedioxythiophene)-1-butyl-3-methylimidazolium hexafluorophosphate (3DOM C 60 -PEDOT-[BMIm][BF 6 ]) for ultrasensitive detection of thrombin by cascade catalysis of [email protected] 2 microspheres and alcohol dehydrogenase (ADH). [email protected] 2 microspheres were constructed not only as nanocarriers to anchor the large amounts of secondary thrombin aptamers but also as nanocatalysts to catalyze the oxidation of ethanol efficiently. Significantly, the electrochemical signal was greatly enhanced based on cascade catalysis: First, ADH catalyzed the oxidation of ethanol to acetaldehyde with the concomitant generation of NADH in the presence of β-nicotinamide adenine dinucleotide hydrate (NAD + ). Then, gold nanoparticles (AuNPs) as nanocatalysts could effectively catalyze NADH to produce NAD + with the help of PEDOT as redox probe. Under the optimal conditions, the proposed aptasensor exhibits a linear range of 2 × 10 −13 to 2 × 10 −8 M with a low detection limit of 2 × 10 −14 M for thrombin detection and shows high sensitivity and good specificity.
研究领域:
分析化学
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生物化学
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纳米技术
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电化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 8
·2403-2409
·
IF 4.831
Ye Cui,Zhong Xia,Wang Min-Qiang,Chai Yaqin,Yuan Ruo
A glassy carbon electrode (GCE) was modified with a nanocomposite consisting of tetraoctylammonium bromide (TOAB), C 60 fullerene, and palladium nanorods (PdNRs). The PdNRs were hydrothermally prepared and had a typical width of 20 ± 2 nm. The nanocomposite forms stable films on the GCE and exhibits a reversible redox pair for the C 60 /C 60
− system while rendering the surface to be positively charged. The modified GCE was applied to fabricate an electrochemical biosensor for detecting acetylcholinesterase (AChE) by measurement of the amount of thiocholine formed from acetylthiocholine, best at a working voltage of −0.19 V (vs. SCE). The detection scheme is based on (a) measurement of the activity of ethyl paraoxon-inhibited AChE, and (b) measurement of AChE activity after reactivation with pralidoxime (2-PAM). Compared to the conventional methods using acetylthiocholine as a substrate, the dual method presented here provides data on the AChE activity after inhibition and subsequent reactivation, thereby yielding credible data on reactivated enzyme activity. The linear analytical range for AChE activity extends from 2.5 U L −1 to 250 kU·L −1 , and the detection limit is 0.83 U L −1 .
Cyclovoltammetric acetylcholinesterase (AChE) activity assay is constructed based on the palladium nanorods composited with functionalized C 60 fullerene (PdNR/C 60 + TOAB), which aims to measure the signal change between ethyl paraoxon-inhibited and subsequent pralidoxime (2-PAM)-reactivated AChE activity.
研究领域:
生物传感
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酶学分析
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纳米材料修饰电极
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电化学检测
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毒理学
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Yan Li,Junlin He,Jun Chen,Yazhen Niu,Yilin Zhao,Yuchan Zhang,Chao Yu
In this study, a dual-type responsive electrochemical immunosensor was developed for the quantitative detection of proprotein convertase subtilisin/kexin type 9 (PCSK9), a potential biomarker of cardiovascular disease in serum. N-doped graphene nanoribbons (N-GNRs) with good conductivity were used as the sensing matrix modifying the glassy carbon electrode. Palladium platinum alloy (PdPt) nanoparticles with high catalytic performance toward the reduction of hydrogen peroxide (H 2 O 2 ) were reduced onto amino-functionalized fullerene (n-C 60 -PdPt) and significantly amplified the electrochemical signal recorded by the amperometric i-t curve. Furthermore, staphylococcus protein A (SPA) with antibody orientation function was introduced to improve the immunoreaction efficiency. Accordingly, a label-free immunosensor was fabricated based on n-C 60 -PdPt/N-GNRs for the quick detection of PCSK9. Meanwhile, to realize ultrasensitive detection of PCSK9, Pt-poly (methylene blue) (Pt-PMB) nanocomposites synthesized by a one-pot method for the first time were used as a novel signal label, which exhibited uniform morphology as well as good conductivity and produced an electrochemical signal recorded by differential pulse voltammetry (DPV). Herein, a novel sandwich-type immunosensor was designed using n-C 60 -PdPt/N-GNRs as the sensing matrix and Pt-PMB as the signal label for sensitive detection of PCSK9. Under optimal conditions, the label-free immunosensor showed a linear range of 10 pg mL −1 to 100 ng mL −1 with a detection limit of 3.33 pg mL −1 (S/N=3), and the sandwich-type immunosensor exhibited a linear range of 100 fg mL −1 to 100 ng mL −1 with a detection limit of 0.033 pg mL −1 (S/N=3) for PCSK9 detection, indicating its potential application in clinical bioassay analysis.
研究领域:
生物医学工程
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分析化学
·
临床检验
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2023
·
IF 6.7
Yuan-Sheng Cheng,Yi-Fei Huang,Xin-Yue Wu,Min Ling,Yuwen Cheng,Fang-Hui Wu,Qiyan Xu,Xian-Wen Wei
As a promising alternative to platinum, molybdenum disulfide (MoS 2 ) is widely utilized as catalyst/co-catalyst in hydrogen evolution reaction (HER). However, its inactive basal plane greatly hampers its catalytic activity. Herein, we propose a function-oriented strategy to enhance the catalytic activity of MoS 2 by interfacing with fullerene derivatives, specifically indene-C 60 bisadduct (ICBA). The ICBA-MoS 2 hybrid was then employed as an efficient co-catalyst, in conjunction with CdS, to construct a photocatalyst for solar-driven hydrogen production coupled with the selective oxidation of furfural alcohol (FOL) to furfural (FAL). The optimized 5 % ICBA-MoS 2 /CdS (5 %-IMC) sample exhibits a remarkable H 2 and FAL production rates of 978 and 1160 μmol h −1 g −1 , respectively, which was 2.4 times higher than that of the primitive sample without ICBA modified. This prominent catalytic performance can be attributed to the high activity of synergistic active sites in ICBA-MoS 2 , as well as the speed transportation and separation of the photogenerated charges from CdS to ICBA-MoS 2 , as evidenced by both experimental and theoretical investigations.
研究领域:
材料科学
·
催化化学
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产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2023
·
IF 6.7
Shijie Sun,Baijin Li,Wei Xiong,Boyu Fu,Yong Zhang,Zilin Ruan,Lei Gao,Xiaoqing Zuo,Jianchen Lu,Jinming Cai
Supramolecular self-assembly offers a feasible route for nanopatterning and functionality. The construction and precise control of selective adsorption at the molecular scale play essential roles in fundamental sciences and practical applications. Molecular tiling such as trihexagonal tiling Kagome networks has potential promising chemical and physical properties. Distorted Kagome-like networks controllable fabrication is possible by coupling molecular functional groups and substrate atoms. A chiral Kagome-like network was constructed using IFDO ([1,2-b]fluorene-6,12-dione) as precursor molecules complemented with molecular beam epitaxy and annealing strategies. Revealing arrangements of the assembled network of O-Cu metal coordination linked Kagome-like networks on the Cu(1 1 1) surface and their assembly mechanisms and cavity characteristics were explored in detail through scanning tunneling microscopy combined with density functional theory. Based on the electronic state difference between the hole center of the network and the molecular backbone, the host–guest network was successfully prepared by co-depositing C 60 molecules. Kagome-like networks could as an efficient template for regulating the adsorption configuration of C 60 . Our results demonstrate a controllable and efficient method to design atomically precise ordered host–guest assemblies on solid surfaces, advancing the approach is yet another step toward the fabrication of molecular devices.
研究领域:
表面科学
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纳米材料学
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超分子化学
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凝聚态物理
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分子自组装
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扫描探针显微学
✓ 已验证
DOI
高影响因子
Nature Chemistry
·
2024
·
IF 19.2
Zhang Yangyu,Peng Xuanbei,Tian Han-Rui,Yang Bo,Chen Zuo-Chang,Li Jiejie,Zhang Tianhua,Zhang Mingyuan,Liang Xiaocong,Yu Zhiyang,Zhou Yanliang,Zheng Lirong,Wang Xiuyun,Zheng Jian-Wei,Tang Yu,Au Chak-tong,Jiang Lilong,Xie Su-Yuan
Developing highly effective catalysts for ammonia (NH 3 ) synthesis is a challenging task. Even the current, prevalent iron-derived catalysts used for industrial NH 3 synthesis require harsh reaction conditions and involve massive energy consumption. Here we show that anchoring buckminsterfullerene (C 60 ) onto non-iron transition metals yields cluster-matrix co-catalysts that are highly efficient for NH 3 synthesis. Such co-catalysts feature separate catalytic active sites for hydrogen and nitrogen. The ‘electron buffer’ behaviour of C 60 balances the electron density at catalytic transition metal sites and enables the synergistic activation of nitrogen on transition metals in addition to the activation and migration of hydrogen on C 60 sites. As demonstrated in long-term, continuous runs, the C 60 -promoting transition metal co-catalysts exhibit higher NH 3 synthesis rates than catalysts without C 60 . With the involvement of C 60 , the rate-determining step in the cluster-matrix co-catalysis is found to be the hydrogenation of *NH 2 . C 60 incorporation exemplifies a practical approach for solving hydrogen poisoning on a wide variety of oxide-supported Ru catalysts.
研究领域:
化学
·
材料科学
·
催化
·
氨生产
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DOI
PubMed
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2013
·Vol. 15
·期 12
·1-11
·
IF 2.175
Li Zhi,Zhang Fei-long,Wang Zhiyuan,Pan Li-li,Shen Ying-ying,Zhang Zhen-zhong
The photocytotoxicity of water-dispersed 100–300 nm fullerene amino acid derivatives nanoparticles was studied. The nanoparticle solution of fullerene derivatives, l -phenylalanine (C60-phe) and glycine (C60-gly), suppressed the in vitro growth of MCF-7 cells lines, induced cancer cells apoptosis, and caused a perturbation of the cell cycle. These nanoparticle solutions increased intracellular reactive oxygen species after irradiation. C60-phe or C60-gly upregulated the expression of phosphorylated (p)p38 mitogen-activated protein kinase (MAPK). N -Acetyl- l -cysteine significantly depressed the composite-induced activation of p38MAPK, and the kinase inhibitor SB203580 significantly prevented C60 derivative-induced cell apoptosis. This study revealed that p38MAPK is activated by C60 nanoparticles through triggering reactive oxygen species generation, leading to cancer cell injuries.
研究领域:
纳米技术
·
癌症生物学
·
细胞生物学
·
药理学
✓ 已验证
DOI
高影响因子
TALANTA
·
2015
·
IF 3.545
Jing Lan,Chunfang Liu,Mingxuan Gao,Chengzhi Huang
In order to achieve the simple, easily repeated, and large scale preparation of fluorescent CDs, a new solid-state synthesis (SSS) approach was developed by calcining the mixture of fullerenes (C 60 ) and solid sodium hydroxide. The cage of fullerenes could be opened and the hydroxyl and carboxyl were successfully introduced in the presence of sodium hydroxide under high temperature. The as-prepared surface carboxylated CDs possess many good properties, such as high water solubility, good photostability, salt tolerance, and nontoxicity. Especially, the fluorescence of CDs could be highly quenched by Fe 3+ because of the strong interaction of hydroxyl or carboxyl on the as-obtained CDs with Fe 3+ , which realized a sensitive detection of Fe 3+ in the linear range of 0.02–0.6 μmol/L. What is more, we further applied the obtained CDs into the intracellular imaging of Fe 3+ .
研究领域:
材料科学
·
生物医学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
FREE RADICAL BIOLOGY AND MEDICINE
·
2024
·
IF 7.1
Haoyu Wang,Junqi Zhao,Shiliang Ji,Tingjun Liu,Zhisheng Chen,Zhen Huang,Yuhui Zang,Jiangning Chen,Junfeng Zhang,Zhi Ding
Alcoholic liver disease (ALD) is a common chronic redox disease caused by increased alcohol consumption. Abstinence is a major challenge for people with alcohol dependence, and approved drugs have limited efficacy. Therefore, this study aimed to explore a new treatment strategy for ALD using ferroferric oxide endohedral fullerenol (Fe 3 O 4 @C 60 (OH) n ) in combination with static magnetic and electric fields (sBE). The primary hepatocytes of 8–9-week-old female BALB/c mice were used to evaluate the efficacy of the proposed combination treatment. A mouse chronic binge ethanol feeding model was established to determine the alleviatory effect of Fe 3 O 4 @C 60 (OH) n on liver injury under sBE exposure. Furthermore, the ability of Fe 3 O 4 @C 60 (OH) n to eliminate •OH was evaluated. Alcohol-induced hepatocyte and mitochondrial damage were reversed in vitro . Additionally, the combination therapy reduced liver damage, alleviated oxidative stress by improving antioxidant levels, and effectively inhibited liver lipid accumulation in animal experiments. Here, we used a combination of magnetic derivatives of fullerenol and sBE to further improve the ROS clearance rate, thereby alleviating ALD. The developed combination treatment may effectively improve alcohol-induced liver damage and maintain redox balance without apparent toxicity, thereby enhancing therapy aimed at ALD and other redox diseases.
研究领域:
药理学
·
毒理学
·
分子生物学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
Carbon Energy
·
2025
·Vol. 7
·期 5
·e70001
·
IF 24.2
Qianfeng Wu,Chuanhao Li,Shuo Chen,Zhenghua Su,Muhammad Abbas,Chao Chen,Qianqian Lin,Jingting Luo,Liming Ding,Guangxing Liang
Visible and near-infrared photodetectors are widely used in intelligent driving, health monitoring, and other fields. However, the application of photodetectors in the near-infrared region is significantly impacted by high dark current, which can greatly reduce their performance and sensitivity, thereby limiting their effectiveness in certain applications. In this work, the introduction of a C 60 back interface layer successfully mitigated back interface reactions to decrease the thickness of the Mo(S,Se) 2 layer, tailoring the back-contact barrier and preventing reverse charge injection, resulting in a kesterite photodetector with an ultralow dark current density of 5.2 × 10 −9 mA/cm 2 and ultra-weak-light detection at levels as low as 25 pW/cm 2 . Besides, under a self-powered operation, it demonstrates outstanding performance, achieving a peak responsivity of 0.68 A/W, a wide response range spanning from 300 to 1600 nm, and an impressive detectivity of 5.27 × 10 14 Jones. In addition, it offers exceptionally rapid response times, with rise and decay times of 70 and 650 ns, respectively. This research offers important insights for developing high-performance self-powered near-infrared photodetectors that have high responsivity, rapid response times, and ultralow dark current.
研究领域:
光电子学
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光探测器
·
材料科学
·
半导体器件
·
可再生能源
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）