Materials
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2018
·Vol. 11
·期 11
·2203
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IF 2.467
Shufang Wu,Xunjun Chen,Minghao Yi,Jianfang Ge,Guoqiang Yin,Xinming Li
The high moisture sensitivity of feather keratin/polyvinyl alcohol/tris(hydroxymethyl)aminomethane (FK/PVA/Tris) blend films hinders their application in the packaging field. Thus, in order to improve the water resistance and mechanical properties of such blend films, we attempted cross-linking the blend film with cross-linking agents such as transglutaminase (TG), CaCl2, and genipin. Obvious differences in the morphology of the blended films were observed by scanning electron microscopy before and after cross-linking, indicating that cross-linking can inhibit the phase separation of the blend film. Conformational changes in the blend films after cross-linking were detected by Fourier transform infrared spectroscopy. Importantly, from examination of the total soluble mass, contact angle measurements, and water vapor permeability tests, it was apparent that cross-linking greatly improved the water resistance of the blend films, in addition to enhancing the mechanical properties (i.e., tensile strength and elongation at break). However, cross-linking was also found to reduce the oxygen barrier properties of the blend films. Therefore, cross-linking appears to be an effective method for promoting the application of FK/PVA/Tris blend films in the packaging field.Keywords:blend film;feather keratin;cross-linking;water resistance;mechanical properties
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高影响因子
American Journal of Translational Research
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2021
·Vol. 13
·期 4
·2111-2126
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IF 4.06
Chao Shi,Chongwen Shen,Ge Liu,Shaofeng Yang,Fenglin Ye,Jinjin Meng,Youmin Pan
Purpose To clarify the regulatory effect of Nuclear-enriched abundant transcript 1 (NEAT1) on abdominal aortic aneurysm (AAA) model rats and isolated endothelial progenitor cells (EPCs). Methods The AAA rat model was established by CaCl 2 stimulation, and overexpressed NEAT1 was injected into rats through tail vein. Abdominal aorta lesions and numbers of EPCs in tissues and peripheral blood were examined by hematoxylin-eosin, immunofluorescence and flow cytometry. The extracted EPCs were identified by microscopy, DiI-ac-LDL staining and flow cytometry. Effect of overexpressed/silencing NEAT1 on the viability, migration, tube formation and VEGF content of EPCs was investigated by MTT-, wound-healing, tube formation assays and ELISA, respectively. The expressions of NEAT1, miR-204-5p, Angiopoietin-1 (Ang-1)/ERK pathway were determined by qRT-PCR and Western blot as needed. The targeting relationships between NEAT1 and miR-204-5p, and miR-204-5p and Ang-1 were predicted on starBase, TargetScan and confirmed by dual-luciferase experiments. The mutual regulation effect was studied through rescue experiments. Results Overexpressed NEAT1 not only reduced inflammatory infiltration and increased the number of EPCs in abdominal aorta and peripheral blood, but also promoted the viability, migration, tube formation of EPCs, increased VEGF content and upregulated the expression of the Ang-1/ERK pathway in EPCs. However, silencing NEAT1 produced opposite results. NEAT1 targeting miR-204-5p inhibited the functional effects of miR-204-5p on of EPCs. Overexpressed/silencing Ang-1 partially reversed the effects of NEAT1 or miR-204-5p on the characteristics of EPCs. Conclusion NEAT1 competitively binds with miR-204-5p and up-regulates Ang-1 expression in EPCs to effectively improve the proliferation, migration and angiogenesis of EPCs.
研究领域:
分子生物学
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心血管疾病
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细胞生物学
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基因调控
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Nanoscale
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2019
·Vol. 11
·期 26
·12537-12546
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IF 6.97
Jiguo Tu,Junxiang Wang,Shijie Li,Wei-Li Song,Mingyong Wang,Hongmin Zhu,Shuqiang Jiao
Highly efficient strategies for the transformation of amorphous carbon into graphite with high graphitization and crystallinity features have been significantly pursued in recent years; however, critical issues, including high processing temperature, insufficient graphitization, introduction of catalyst impurities, complicated post-purification procedures, and generation of greenhouse gas, still remain in traditional approaches. For significantly addressing these challenges, herein, a highly efficient catalyst-free, eco-friendly and low-temperature electrochemical transformation strategy was proposed for the preparation of highly graphitized porous graphite nanoflakes. Using inert SnO2 as an anode in CaCl2-LiCl molten salts, the graphitization transformation of amorphous carbon materials could be realized at 700 °C, approaching the record in high-efficiency converting amorphous carbon to graphite; moreover, systematical analysis was performed to understand the electrochemical transformation of amorphous carbon into highly graphitized graphite nanoflakes. For extending their valuable applications, the as-prepared graphite nanoflakes were further utilized as cathodes in aluminum-ion batteries, which exhibited significantly promising energy storage performance; moreover, an initial discharge capacity of 63.6 mA h g−1 at a current density of 200 mA g−1 was achieved, which eventually became 55.5 mA h g−1 with a coulombic efficiency of 95.4% after 1000 cycles; thus, these cathodes exhibited stable long-term cycling performance. The combination of low-temperature electrochemical transformation and the subsequent high-performance applications of these nanoflakes in energy storage indicates that the proposed strategy is highly efficient for the transformation and utilization of abundant amorphous carbon resources for the realization of high value-added applications.
研究领域:
材料科学
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电化学
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能量存储
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纳米技术
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高影响因子
Journal of Functional Foods
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2014
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IF 4.48
Chun-Feng Guo,Jing-Yan Li
The effects of a Tween 80 and CaCl 2 mixture on the hypocholesterolemic activity of bile salt hydrolase (BSH)-active Lactobacillus casei F0422 were investigated in rats fed a cholesterol-rich diet for 3 weeks. This mixture significantly enhanced the hypocholesterolemic activity of strain F0422 in rats. The group administered drinking water supplemented with both strain F0422 and the Tween 80 and CaCl 2 mixture had significantly lower levels of total serum cholesterol, low-density lipoprotein cholesterol and total liver cholesterol than did the group administered drinking water supplemented only with strain F0422 or the Tween 80 and CaCl 2 mixture. The hypocholesterolemic activity of the combined strain F0422 and Tween 80 and CaCl 2 mixture was attributed to the increase in fecal bile acid excretion through enhancement of bile salt tolerance and BSH catalytic activity of strain F0422 by Tween 80 and up-regulation of the adhesion of strain F0422 to intestinal epithelial cells by CaCl 2 .
研究领域:
营养学
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微生物学
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药理学
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高影响因子
Journal of Bionic Engineering
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2024
·
IF 4.9
Fu Huide,Wang Ben,He Rui,Yang Yongpu,Li Hongyuan,Guo Zhiguang
Photovoltaic (PV) power generation is highly regarded for its capability to transform solar energy into electrical power. However, in real-world applications, PV modules are prone to issues such as increased self-heating and surface dust accumulation, which contribute to a reduction in photoelectric conversion efficiency. Furthermore, elevated temperatures can adversely affect the components’ operational longevity. To augment the efficiency and extend the lifespan of PV modules, it is crucial to implement cooling strategies and periodic surface dust removal. In this research, we introduce a composite PV module design that amalgamates a hygroscopic hydrogel with self-cleaning attributes. The design incorporates a superhydrophobic polydimethylsiloxane (PDMS) film as its exposed surface layer and employs a PAM-CaCl 2 -SiC hygroscopic hydrogel for rear cooling. This arrangement is intended to facilitate efficient surface self-cleaning and passive cooling of the composite PV module. Experimental studies were conducted to evaluate the performance of this innovative composite PV module design, and the results showed that the composite PV panel had an increase of about 1.39% in power generation compared to an ordinary PV panel in the spring of Shenzhen, China.
研究领域:
可再生能源
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材料科学
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环境工程
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能源工程
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2020
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IF 4.389
Xiaoming Luo,Haixing Song,Jing Yang,Bin Han,Ye Feng,Yanbing Leng,Zhaoqiong Chen
Escherichia coli strain Nissle 1917 (EcN) has been widely shown to effectively treat inflammatory bowel diseases (IBDs). Unfortunately, after oral administration, EcN viability dramatically decreases due to severe environmental factors, including low gastric pH, temperature and osmotic pressure. To address these challenges and improve oral bio-availability, this study utilized layer-by-layer assembly (LbL) and ionic cross-linking with CaCl 2 as a method of EcN encapsulation (GEcN). Upon examination, GEcN cells were shown to maintain their ability to grow and proliferate, but had a slightly longer stationary phase (10 h) relative to free EcN (4 h). When exposed to simulated gastric fluid (SGF), a higher number of GEcN cells survived up to 12 h when compared to the other groups. To assess the therapeutic effect of EcN encapsulation in vivo , a TNBS-induced colitis rat model was established. When compared with the oral administration of free EcN, GEcN exhibited a significantly enhanced anti-inflammatory effect. Furthermore, GEcN treatment showed a lower disease activity index (DAI), decreased pro-inflammatory cytokine expression (MPO, TNF-α, IL-6) and increased anti-inflammatory cytokine expression (IL-10). Additionally, rats that received GEcN had much higher ZO-1 expression levels. These results suggest that EcN encapsulation in a chitosan―alginate matrix when utilizing the LbL assembly with CaCl 2 cross-linking can improve probiotic viability in a gastric environmental and thereby offer a more effective treatment for IBDs.
研究领域:
药学
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生物技术
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胃肠病学
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高影响因子
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
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2023
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IF 3.4
Xiaoyan He,Jiwei Liu,Tao Song,Yiying Sun,Xiaoyan Lu,Nuannuan Li,Kaoxiang Sun
A satisfactory drug release profile for gonadotropin-releasing hormone (GnRH) agonist drugs is high initial release followed by small amount of drug release per day. In the present study, three water-soluble additives (NaCl, CaCl2 and glucose) were selected to improve the drug release profile of a model GnRH agonist drug-triptorelin from PLGA microspheres. The pore manufacturing efficiency of the three additives was similar. The effects of three additives on drug release were evaluated. Under the optimal initial porosity, the initial release amount of microspheres containing different additives was comparable, this ensured a good inhibitory effect on testosterone secretion in the early stage. For NaCl or CaCl2 containing microspheres, the drug remaining in the microsphere depleted rapidly after the initial release. The testosterone concentration gradually returned to an uncontrolled level. However, for glucose containing microspheres, it was found that the addition of glucose could not only increase the initial release of the drug but also assist in the subsequent controlled drug release. A good and long-time inhibitory effect on testosterone secretion was observed in this formulation. The underlying cause why the incorporation of glucose delayed the subsequent drug release was investigated. SEM results showed that considerable pores in glucose containing microspheres were healed during the microspheres incubation. After thermal analysis, an obvious glass transition temperature (Tg) depression was observed in this formulation. As Tg decreased, polymer chains are able to rearrange at lower temperatures. This, morphologic change was reflected in the gradual closure of the pores, and is the likely reason that drug release slowed down after the initial release.HIGHLIGHTSThe addition of glucose could not only increase the burst release of the drug but also delay the subsequent drug release.High initial burst and a sustained drug release helped obtain a good inhibitory effect on testosterone secretion.As Tg decreased, polymer chain was prone to rearrange. Morphologic change was reflected in the gradual closure of the pores. This was the reason that drug release slowed down after the initial burst.
研究领域:
药剂学
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药物递送系统
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药理动力学
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CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Lina Chen,Xuelong Chen,Baozheng Cui,Jingbo Zhu,Ningjing Bai,Wei Wang,Huixin Wang,Zewen Li,Haijun Niu,Zhe Wang
In recent years, solar energy interfacial evaporation technology has been extensively explored for seawater desalination and wastewater treatment. However, salt accumulation and complex fabrication of evaporator largely constrains the applications of this technology in practice. Here, one type of interpenetrating polymer network hydrogel composing of agarose (AG) and polyvinylpyrrolidone (PVP) using polypyrrole (PPy) as the light absorbent was fabricated via “hot-ice” template method, which was featured with pitted-surface and microchannels structure. The evenly arranged pits on the surface of the hydrogel can absorb the sunlight as much as possible and convert it into heat energy, improving the efficiency of photothermal absorption. The water channels generated by the formation of CaCl 2 ·6H 2 O crystals can transport water from the bottom to the evaporation interface elevating the transmission rate and accelerating the exchange of gradient salt concentration. As consequences, excellent salt resistance of the hydrogel can be obtained, achieving seawater evaporation rate as high as 3.2 kg m −2 h −1 . In addition, the outstanding ion rejection performance, strong acid/alkali purification capability, and good treatment capacity for heavy metal ions and dye wastewater were also demonstrated.
研究领域:
环境科学
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材料科学
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化学工程
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水处理
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Hui Wang,Jueying Yang,Wei Tian,Kelin Peng,Yun Xue,Haosen Zhao,Xilan Ma,Rui Shi,Yu Chen
Recently, injectable dual-crosslinked (DC) hydrogel scaffolds have attracted many attentions as a class of excellent bone regeneration biomaterials with in-situ tunable functions. However, the design of injectable DC hydrogels with cell behavior-compatible network structure and mechanical property remains a bottleneck. Herein, based on the in-situ gelling method, we constructed an injectable CMCS/PEG+SA/CaCl 2 (CPSC) chemical/physical DC hydrogel scaffold with tunable softness/hardness mechanical properties and good biocompatibility. The formation mechanism and properties of the CPSC hydrogel scaffold were investigated by FTIR , XRD, rheometry, and mechanical testing. It is found that proper softness/hardness mechanical properties can be obtained by adjusting the secondary network structure of the hydrogel. The CPSC hydrogel scaffold prepared under optimal conditions can effectively promote cell infiltration , nutrient transport, and the osteogenic differentiation of rat bone mesenchymal stem cells (rBMSCs). The in vivo experiments show that the rBMSCs-loaded injectable CPSC hydrogels with appropriate mechanical properties can effectively promote bone reconstruction . This study has provided important guidance for the construction of injectable DC hydrogels with adjustable softness/hardness to promote osteogenesis for bone defect repair.
研究领域:
材料科学
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生物材料
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组织工程
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生物医学工程
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骨再生
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PubMed
高影响因子
危险品化学品经营许可证（带存储）