INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Lingling Sha,Husnain Raza,Chengsheng Jia,Imran Mahmood Khan,Huqing Yang,Gang Chen
Polysaccharide-protein nanocomplexes are considered one of the desired systems to encapsulate bioactive compounds. The study prepared chitosan (CS)/genipin (GP)/zein nanoparticles with chemical cross-linkage for encapsulating and releasing curcumin. Fourier transform infrared spectra demonstrated that cross-linkage between molecules was attributed to electrostatic interactions and the formation of amido bonds and hydrogen bonding. Transmission electron microscopy showed that all prepared nanoparticles showed a spherical morphology. Particularly, cinnamaldehyde screening –NH 2 groups in CS significantly reduced the encapsulation efficiency of nanoparticles, demonstrating the significant role of the –NH 2 group in CS-coated zein nanoparticles. Additionally, the encapsulation efficiency was largely increased to 82.3 % at the ratio of CS/GP/zein ( w /w, 20:1:100), compared with 38.4 % using zein as a single encapsulation for curcumin. In vitro digestion, the addition of GP and CS decreased the release of curcumin from 84.8 % to 57.1 % after the initial digestion of 2 h. The GP/CS/zein particles retained 12 % of curcumin after 6- h of digestion, indicating the improvements in encapsulation efficiency and release properties.
研究领域:
食品科学
·
生物材料学
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 14
·8542-8549
·
IF 3.108
Zhenliang Li,Zunli Mo,Shujuan Meng,Huhu Gao,Xiaohui Niu,Ruibin Guo,Taibao Wei
A simple chiral electrochemical sensor based on hydroxypropyl chitosan (HPCS) covalently bound to multi-walled carbon nanotubes (MWCNTs) was developed for the recognition of mandelic acid (MA) enantiomers. HPCS preferably combines with (S)-MA over (R)-MA, which was attributed to the favorable host–guest interactions between HPCS and the MA enantiomers. In addition, the recognition efficiency was significantly increased after hydroxypropyl groups were grafted onto chitosan (CS), which may be due to a stereo-hindrance effect. Moreover, the electrochemical recognition of amino acid enantiomers was investigated; the functional groups of the enantiomers play an important role in the enantioselective discrimination.
研究领域:
生物化学
·
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
MATERIALS & DESIGN
·
2024
·
IF 7.6
Yifan Lu,Ze-Yu Wang,Jianhong Xiao,Zhengyi Zhao,Xiaoyu Liao,Huai-Ling Gao,Duohong Zou
Treatment of osteochondral (OC) defects is still an immense challenge in clinic due to lack of satisfactory strategy to simultaneously regenerate both cartilage and subchondral bone. Herein, we propose an effective bottom-up strategy by developing a type of multifunctional chitosan-based nanocomposite microgels as the building blocks to repair OC defects. The microgels are biocompatible, and biodegradable, and have a hierarchical nanofibrous structure, mimicking the structural feature of extracellular matrix (ECM) to facilitate cell attachment and proliferation. Additionally, β-tricalcium phosphate (β-TCP) nanoparticles are incorporated in the microgels not only to promote the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), but also to facilitate the loading of transforming growth factor beta 1 (TGF-β1). For reconstructing OC defects, pure microgels are implanted at the bottom layer of the OC defects in rat knee joints to promote osteogenesis, while the microgels loaded with TGF-β1 are implanted at the top layer of the OC defects to promote cartilage regeneration. This modular microgel system results in simultaneous regeneration of cartilage and subchondral bone in OC defects, which is expected as a promising way for repairing other complex tissue defects in the field of tissue engineering.
研究领域:
组织工程
·
生物材料
·
再生医学
✓ 已验证
DOI
高影响因子
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
·
2023
·
IF 2.8
Chang Chun-hui,Han De-en,Ji Yu-ying,Wang Meng-yan,Li Dong-hong,Xu Zhi-ling,Li Jia-hao,Huang Sheng-nan,Zhu Xia-li,Jia Yong-yan
Background: Although quercetin exhibits promising anti-tumor properties, its clinical
application is limited due to inherent defects and a lack of tumor targeting.
Objective: This study aimed to prepare and characterize active targeting folate-chitosan modified
quercetin liposomes (FA-CS-QUE-Lip), and its antitumor activity in vitro and in vivo was also
studied.
Methods: Box-Behnken Design (BBD) response surface method was used to select the optimal
formulation of quercetin liposomes (QUE-LP). On this basis, FA-CS-QUE-LP was obtained by
connecting folic acid chitosan complex (FA-CS) and QUE-LP. The release characteristics in vitro
of QUE-LP and FA-CS-QUE-LP were studied. Its inhibitory effects on HepG2 cells were studied
by the MTT method. The pharmacokinetics and pharmacodynamics in vivo were studied in
healthy Wistar mice and S180 tumor-bearing mice, respectively.
Results: The average particle size, zeta potential and encapsulation efficiency of FA-CS-QUELP
were 261.6±8.5 nm, 22.3±1.7 mV, and 98.63±1.28 %, respectively. FA-CS-QUE-LP had a
sustained release effect and conformed to the Maloid-Banakar release model (R2=0.9967). The
results showed that FA-CS-QUE-LP had higher inhibition rates on HepG2 cells than QUE-Sol
(P<0.01). There was a significant difference in AUC, t1/2, CL and other pharmacokinetic parameters
among QUE-LP, FA-CS-QUE-LP, and QUE-Sol (P<0.05). In in vivo antitumor activity
study, the weight inhibition rate and volume inhibition rate of FA-CS-QUE-LP were 30.26% and
37.35%, respectively.
Conclusion: FA-CS-QUE-LP exhibited a significant inhibitory effect on HepG2 cells, influenced
the pharmacokinetics of quercetin in mice, and demonstrated a certain inhibitory effect on S180
tumor-bearing mice, thus offering novel avenues for cancer treatment.
研究领域:
药学
·
肿瘤学
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 43
·2105348
·
IF 18.808
Yanhuizhi Feng,Huai-Ling Gao,Di Wu,Yu-Teng Weng,Ze-Yu Wang,Shu-Hong Yu,Zuolin Wang
Mucogingival surgery has become a common procedure for soft gingival tissue reparation in dental clinical practice, which mainly relies on autograft or commercial collagen membranes (CM). However, the autograft faces grand challenges in source availability and long-term post-surgery pain management, and the CM is restricted by its poor mechanical properties in an aqueous environment. Here, it is reported that a bio-inspired lamellar chitosan scaffold (LCS) with long range ordered porous structure, manufactured through a bidirectional freezing method, can serve as a promising gingival tissue engineering material. The LCS not only exhibits excellent mechanical properties in the hydrated state but also accelerates vessel formation and soft tissue regeneration in vivo. Most interestingly, the LCS is found to be capable of inducing macrophage differentiation to M2 macrophages, which is thought to play an important role in tissue regeneration. These advantages combined with its easy and low-cost preparation process make the LCS a promising candidate for dental clinical applications.
研究领域:
口腔临床实践
·
组织工程
·
生物医用材料
✓ 已验证
DOI
高影响因子
Biomaterials Science
·
2025
·
IF 5.7
Feng Feng,Xing Ge,Tianye Fang,Kaiyang Chen,Jiaying Zhu,Jun Yang,Lei Tao,Jianjun Dai,Yanmin Ju
Dermatophytosis is a complex and prevalent fungal skin infection that affects a significant portion of the global population. Conventional antifungal therapies are often compromised by their inability to effectively penetrate fungal biofilms and limited skin permeability, ultimately leading to unsatisfactory clinical results. To address these issues, we proposed a novel dissolvable microneedle (MN) patch (denoted as TB/QCS MNs) that encapsulated terbinafine (TB) within the quaternary ammonium chitosan (QCS) MN tips. The combination of QCS and TB exhibited a pronounced synergistic effect against Trichophyton mentagrophytes (T. mentagrophytes). Importantly, TB/QCS MNs effectively disrupted fungal biofilms and damaged extracellular polymeric substances. In comparison to TB alone, TB/QCS MNs effectively accelerated fungal clearance, shortened treatment time, and enhanced skin recovery in T. mentagrophytes-infected C57BL/6 mice. These findings demonstrated that TB/QCS MNs significantly improve therapeutic efficacy for dermatophytosis, offering a promising alternative for clinical treatment of skin fungal infections.
研究领域:
药剂学
·
真菌学
·
生物医学工程
·
皮肤科学
·
高分子科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Chaoqun Li,Yongyan Yang,Ruiting Zhang,Jia Wang,Shuangling Zhong,Xuejun Cui
Antibacterial hydrogel film can serve as food packaging materials to prevent bacteria growth and spread, thereby extending shelf life and improve food safety. In this study, an efficient antibacterial hydrogel film (CLG) was prepared with chitosan, lysine, and gelatin. The light transmission of the CLG hydrogel film was over 80 % in the visible region, facilitating the observation of chicken breast storage conditions. Additionally, the swelling ratios of the hydrogel films decreased with increasing gelatin concentration, from 145.7 g/g (CLG1) to 92.6 g/g (CLG2) and 81.5 g/g (CLG3). This reduction was attributed to the denser network structure formed by the interaction between gelatin and the CL polymer. The Scanning Electron Microscopy (SEM) showed that the water-absorbed CLG hydrogel had a unique sponge shape. Moreover, the CLG hydrogel film exhibits high antibacterial activity against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ). In a practical storage experiment, the CLG hydrogel film extended the shelf life of chicken breast by up to 4 days compared to untreated samples, while effectively reducing total volatile basic nitrogen (TVB-N) levels. This hydrogel film is expected to become a promising food packaging material.
研究领域:
食品科学
·
生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Jiaying Zhang,Sifang Zhao,Yifan Zhou,Hongze Liang,Lingling Zhao,Hui Tan
Diabetic wound healing is hampered due to oxidative stress, exacerbated inflammation, and impaired angiogenesis in the wounds. A pH-sensitive antioxidant hydrogel based on carboxymethyl chitosan (CMCS), oligoprocyanidins (OPC), and oxide dextran (Oxd) is prepared to accelerate diabetic wound healing. The hydrogel network is formed via imine and hydrogen bonding interactions in the presence of hydroxyl, amino, and aldehyde groups, and deferoxamine (DFO) is incorporated into the hydrogel. The hydrogel shows pH-triggered drug release, a good antioxidant, and anti-inflammatory properties, and can promote tube formation and cell migration in vitro. Moreover, the hydrogel can accelerate wound healing in streptozotocin (STZ)-induced diabetic mice by regulating the inflammation environment with up-regulation of anti-inflammatory factors (IL-4 and IL-10) and down-regulation of pro-inflammatory factors (TNF-α and IL-6), and promoting angiogenesis with up-regulation of HIF-1α, VEGF, and CD31. Thus, the pH-sensitive antioxidant hydrogel provides a promising therapeutic strategy for diabetic wound healing.
研究领域:
生物材料学
·
医学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xiangru Han,Liguang Tian,Qiuhong Chen,Linqing Wang,Yingqi Mi,Qing Li,Zhanyong Guo,Fang Dong
The aim of this study was to synthesize thiolated chitosan derivative and evaluated its antioxidant activity. Thiol groups were introduced into chitosan and hydroxypropyl trimethylammonium chloride chitosan quaternary ammonium salt (HACC) by microwave reaction, respectively. At the same time, a series of compounds containing thiol groups were introduced at the amino site. The structure of the chitosan derivatives was identified by FTIR and 1 H NMR. The thiol groups content, thermal stability, water solubility and cytotoxicity of the chitosan derivative were evaluated. The antioxidant activity of chitosan derivatives was evaluated using superoxide anion radical scavenging activity assay, DPPH radical scavenging activity assay, hydroxyl radical scavenging activity assay and reducing power assay. The results showed that the thiolated chitosan derivatives obtained in this study possessed excellent antioxidant activity, which provides a new idea for the further study of chitosan antioxidants.
研究领域:
生物化学
·
材料科学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomedical Materials
·
2024
·Vol. 19
·期 4
·045042
·
IF 3.9
Ling Wang,Xiaoyue Ding,Jingyu Li,Man Li,Peng Ding,Wei Guo,Qiaoyun Wu,Yanfang Sun,Guohua Jiang,Oseweuba Valentine Okoro,Mahta Mirzaei,Amin Shavandi,Lihong Fan,Lei Nie
Bacterial infection can lead to various complications, such as inflammations on surrounding tissues, which can prolong wound healing and thus represent a significant clinical and public healthcare problem. Herein, a report on the fabrication of a novel genipin/quaternized chitosan (CS) hydrogel for wound dressing is presented. The hydrogel was prepared by mixing quaternized CS and genipin under 35 °C bath. The hydrogels showed porous structure (250–500 μm) and mechanical properties (3000–6000 Pa). In addition, the hydrogels displayed self-healing ability and adhesion performance on different substrates. Genipin crosslinked quaternized CS hydrogels showed antibacterial activities against E. coli and S. aureus. The CCK-8 and fluorescent images confirmed the cytocompatibility of hydrogels by seeding with NIH-3T3 cells. The present study showed that the prepared hydrogel has the potential to be used as wound dressing.
研究领域:
生物材料学
·
医学工程
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yanan Huang,Xiaotong Wang,Bodan Luo,Peng Jin,Yonghua Zheng,Changliang Xu,Zhengguo Wu
Effective control of wound bleeding and sustained promotion of wound healing remain a major challenge for hemostatic materials. In this study, the hemostatic sponge with controllable antibacterial and adjustable continuous promotion of wound healing (CMN Cu) was prepared by chitosan, aminated MXene and copper ion . Interestingly, the internal topological point-line-surface interaction endowed the CMN-Cu sponge longitudinal staggered tubular porous microstructure, combined with the lipophilic properties obtained by modified MXene, which greatly improved its flexibility, wet elasticity and blood enrichment capacity. In addition, the sponge achieved controlled release of active ingredients, which made it present highly effective antibacterial activity and long-lasting ability to promote wound healing. In vitro and in vivo experiments confirmed that CMN-Cu sponge presented high-efficient hemostatic performance. Last but not least, a series of cell experiments showed that the CMN-Cu sponge had excellent safety as a hemostatic material.
研究领域:
生物医学材料
·
创伤修复
·
药物释放系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jie Ren,Zengyang Wu,Bai Wang,Liuping Zheng,Siyu Han,Jianshe Hu
Chitosan-based hydrogels, as natural high-molecular-weight flexible materials, are widely utilized due to their outstanding properties. In this research, we developed a one-pot method for synthesizing a novel PVA/CS@PPy-PDA x% conductive hydrogel and explored the internal bonding patterns through molecular dynamics simulations. By adding PPy-PDA nanoparticles into a hydrogel matrix, an interpenetrating conductive network established successfully. The uniform distribution of PPy-PDA nanoparticles endowed the hydrogel with good electrical conductivity (0.171 S/m), significantly enhanced mechanical properties, and strain sensing (S = 5.04), as well as near-infrared photothermal responsiveness (temperature increase of 41.9 °C within 30 s). Additionally, due to the hydrogel's significant photothermal conversion efficiency under near-infrared radiation, it exhibits rapid elimination of Escherichia coli with an antibacterial efficiency exceeding 90 %. The unique hydrogen-bonded crosslinked structure provides the hydrogel with excellent re-healing properties, allowing for restoration through a freeze-thaw process after damage. The conductivity remains nearly unchanged after re-healing, maintaining the material's integrity and functionality. The flexible sensor based on this hydrogel has a response time of 100 ms and can sensitively detect large-scale deformations (e.g., joint bending at various angles), different gravitational forces, and recognize human handwriting. These characteristics make this hydrogel a promising candidate for advancing intelligent wearable technologies and human-machine interaction systems.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）