NEW JOURNAL OF CHEMISTRY
·
2024
·
IF 2.7
Qingtai Chen,Chongchong Wu,Shujie Hu,Lizhi Cui,Yong Zhang,Peng Hu,Zhi Xu,Pei Yu,Miao Yu
Xylanase is a natural and high-efficiency catalyst for the degradation of xylan to reducing sugar. However, the catalytic performance of xylanase is greatly limited by its poor activity and stability at the harsh condition. To overcome this issue, covalent immobilization of xylanase on a magnetic porous MOFs material with core-shell structure, UIO-66-NH2 carrier was applied to improve the catalytic activity and stability of xylanase. Several tools were employed to monitor the preparation and immobilization processes, magnetic properties, and the structural/chemical characteristics of the biocatalyst. 77.9% of the activity for xylanase was retained after immobilization. Moreover, the thermal stability in terms of half-life of the immobilized enzyme was three times higher than that in free form, and which had higher catalytic efficiency for hydrolysis of xylan. Furthermore, the greater temperature and wider pH ranges of the activity stability were achieved for the immobilized xylanase in compared with free one. Notably, the biocatalyst exhibits both excellent storage stability and could be easily separated using a magnet, which is conducive for its reusability. And nearly 70% of its initial activity was retained after seven recycles during the hydrolysis of corn straw. These features indicate that the present immobilization method is promising prospect for the industrial application of enzyme.
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Food Chemistry-X
·
2024
·
IF 6.5
Zuoyong Zhang,Jiayi Chen,Li Zheng,Jinlong Zhao,Na Guo,Xue Fang,Xuan Lu,Fangyan Zhang,Guilan Zhu
Maillard reaction products (MRPs) derived from rice protein isolate hydrolysate and D-xylose, with or without L-cysteine, were developed as a potential meat flavoring. The combined impact of temperature (80–140 °C) and cysteine on fundamental physicochemical characteristics, antioxidant activity, and flavor of MRPs were investigated through assessments of pH, color, UV–visible spectra, fluorescence spectra, free amino acids, volatile compounds, E -nose, E-tongue, and sensory evaluation. Results suggested that increasing temperature would reduce pH, deepen color, promote volatile compounds formation, and reduce the overall umami and bitterness. Cysteine addition contributed to the color inhibition, enhancement of DPPH radical-scavenging activity and reducing power, improvement in mouthfulness and continuity, reduction of bitterness, and the formation of sulfur compounds responsible for meaty flavor. Overall, MRPs prepared at 120 °C with cysteine addition could be utilized as a potential meat flavoring with the highest antioxidant activity and relatively high mouthfulness, continuity, umami, meaty aroma, and relatively low bitterness.
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PubMed
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Molecular Catalysis
·
2024
·
IF 3.9
Yuqiu Wu,Fanjin Meng,Jiaolong Fu,Yuyang Zhou,Huan Fan,Xin Ju,Liangzhi Li
D-xylose, a biomass derivative, is used as a substrate to generate D-xylulose and D-ribulose with high-added value through a cascade reaction. During this process, the thermal stability and catalytic efficiency of rate-limiting enzymes during cascade reactions are important issues that must be addressed in industrial applications. In this study, we prepared and validated PDA/SiO 2 microspheres as highly loaded immobilization carriers by oxidative self-polymerization of polydopamine (PDA) on silica (SiO 2 ) microspheres. D-xylose was successfully catalyzed by the co-immobilized D-5-phosphate ribose isomerase B (RpiB) and D-tagatose-3-epimerase (DTE), denoted PDA/SiO 2 -RpiB-DTE, to produce D-ribulose and D-xylulose. After immobilization, the K m of co-immobilized RpiB and DTE decreased, which suggested increased substrate affinity. The half-life of the co-immobilized enzymes was 3.5–8 fold higher than that of the free enzymes at 50–70 °C, and the inactivation rate constant was reduced, demonstrating that immobilization improved significantly the thermal stability. The conversion of D-xylose at 10 mM was 29.98% under optimal conditions, which contained 9.85% D-ribulose and 20.13% D-xylulose. PDA/SiO 2 -RpiB-DTE showed up to 89.10% initial enzyme activity after 3 recycle runs. The thermal stability and recovery aspects were improved by immobilization, which facilitates the use of biomass to produce high-value-added products and further exploitation.
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COMPOSITES PART B-ENGINEERING
·
2025
·
IF 14.2
Yifan Gui,Gen Lu,Chengyu Sun,Ya Wu,Xu Wang,Yuanmin Zhu,Wenwen Jin,Longjiang Yu
In this work, graphene oxide (GO) composites, sequentially enhanced with polydopamine (PDA) and polyethyleneimine (PEI), were developed for the innovative immobilization of xylose dehydrogenase (XylB). These composites were designed to achieve positively charged surfaces, aiming to explore the potential interactions between the enzyme and the modified composites. The focus was particularly on determining whether these modifications could improve the enzyme's catalytic performance and stability. Characterization was performed using Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Photoelectron Spectroscopy (XPS), and Scanning Electron Microscopy (SEM). Molecular dynamics simulations elucidated the structure-function correlations of immobilized XylB, revealing significant enhancements in thermal stability and catalytic performance compared to its free counterpart. Notably, the immobilized enzyme retained over 85 % of its activity after four consecutive usage cycles and exhibited double the residual activity compared to free XylB after 30 days of storage at 4 °C. Additionally, the immobilized system achieved a 1.4-fold increase in xylonic acid yield relative to the free system. This approach not only highlights the potential of advanced material composites in industrial enzyme applications but also sets a foundation for further research into sustainable biocatalyst technologies.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Yuvaraj Ravikumar,Abdullah Arsalan,Xinrui Tang,Guoyan Zhang,Xianghui Qi
l-Arabinose isomerase (l-AI) catalyzes d-galactose to produce the rare, industrially important sugar d-tagatose. Enzyme stability is vital for its application in industrial processes, and rational design-based protein engineering methods have been employed to improve its stability. This study employed molecular dynamics simulations (MDS)-guided rational redesign of flexible regions to improve the thermostability of mesophilic Bifidobacterium adolescentis l-AI (Ba-l-AI). Comparative MDS (200 ns, 340–360 K) of thermophilic l-AIs (Geobacillus kaustophilus Gk-l-AI and Thermotoga maritima Tm-l-AI) and mesophilic Ba-l-AI identified flexible regions in Ba-l-AI via RMSF and ionic interaction. Five stabilizing mutations (G78C, K46R, N187R, K112R, and N190R) were introduced to provide rigidness. MDS of the mutant revealed reduced RMSF/RMSD, improved compactness, and enhanced conformational stability across temperatures, supported by free-energy landscape analysis resembling thermophilic profiles. Experimentally, the mutant exhibited superior thermal stability (72 °C), broader pH/temperature tolerance, and efficient d-tagatose production. The redesigned Ba-l-AI’s rigid backbone and stability highlight its potential for industrial d-tagatose biosynthesis.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Jiahui Wu,Xin Li,Xiaoning Huang,Yun Zhu,Hongquan Yu,Yingjie Xia,Suisui Guo,Xiaoyang Wang,Tingxia Dong,Karl Wah Keung Tsim,Yue Zhu
Ethnopharmacological relevance The gut microbiome plays a crucial role in the pathology of depression. The intestinal dysbiosis associated with prolonged use of antidepressants, such as fluoxetine, can adversely affect the efficacy of these medications. Kai-Xin-San (KXS), a traditional Chinese herbal decoction, has been utilized to treat mental disorders with a long history in China. The modulation of the gut microbiome by KXS could underlie its antidepressant effect. In the context of combining with fluoxetine, KXS could potentially mitigate fluoxetine-associated intestinal side effects during depression treatment. Aim of the study This study investigates the impact of KXS on the gut of depressive mice, with a particular emphasis on its potential to mitigate fluoxetine-induced intestinal side effects. Materials and methods A high dose of fluoxetine was applied to the chronic unpredictable mild stress (CUMS)-induced mice, alone or in combination with KXS. Behavior tests were conducted to confirm the anti-depressant efficiencies. The feces of mice were collected and subjected to 16S rDNA and metagenomic sequencing. The gastrointestinal morphology and functions were assessed. The potential mechanistic action of KXS on alleviating the intestinal dysbiosis was probed. Results Notable imbalance of microbiome and disruption of intestinal barrier were observed in CUMS mice. The intake of fluoxetine exacerbated the dysbiosis, as evidenced by the increased ratio of Firmicutes/Bacteroidetes and the elevated abundance of antibiotic-resistant genes in the gut microbiome. In addition, fluoxetine treatment further compromised the intestinal integrity and functions. Significantly, KXS treatment effectively mitigated the impairment of intestinal barrier induced by fluoxetine. These protective effects appeared to be mediated through multiple mechanisms, including the restoration of microbial homeostasis and the direct cytoprotective action on intestinal epithelial cells. Conclusions These findings particularly provide support for the combined usage of KXS and fluoxetine in depression treatment.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Bingquan Chu,Weiyi Jin,Cheng Feng,Xiaohua Cui,Lin Gan,Guanghua He,Gongnian Xiao,Genxiang Mao
The bioactive molecules of Dendrobium officinale , an edible medicinal plant native to China, comprise mainly polysaccharides, which exhibit diverse physiological activities. However, the rigid cell wall structure of D. officinale hinders their efficient release. Steam explosion (SE) is an effective pretreatment technology for disrupting the cell walls of raw materials. In this study, the effects of SE at severity factors (SF) of 3.50 and 3.91 on the yield of D. officinale polysaccharides were evaluated. Additionally, the structural features, chemical composition, and antioxidative activities of polysaccharides from SE-treated D. officinale (SEDOPs) before and after in vitro digestion were analyzed. Compared with the untreated group (DOP), the SE-treated samples exhibited enhanced polysaccharide release rates. Additionally, SEDOP 3.50 was significantly purer and had a higher uronic acid content than that of DOP. SE treatment also reduced molecular weights of the polysaccharides. Mannose and glucose were identified as the predominant monosaccharides, with SE altering their molar ratios. Structurally, the SEDOPs developed porous architectures while their primary structures remained preserved. Their triple-helical conformations were disrupted following intestinal digestion. Furthermore, SE treatment enhanced their antioxidative capacities, with SEDOP 3.50 consistently exhibiting the highest uronic acid content and strongest antioxidative activity throughout the digestion process.
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DESALINATION
·
2025
·
IF 9.8
Tong Wei,Kai Li,Senlin Shao,Gang Wen,Jianwei Bu,Tinglin Huang
Nanofiltration membranes (NFMs) are promising candidates for advanced drinking water treatment due to their excellent retention capabilities, however, their widespread application is limited by low water permeance and susceptibility to inorganic scaling. In this study, Fe(III)-tannic acid‑molybdenum disulfide (Fe(III)-TA-MoS 2 ) nanosheets were synthesized and employed as an interlayer material for NFM fabrication. The resulting NFMs were then created through interfacial polymerization, labeled as MoS 2 -1.5, MoS 2 -3.0, and MoS 2 -4.5 based on varying materials loading. Experimental results indicated that the membrane morphology, surface roughness, electronegativity, amine group content, hydrophilicity, selectivity, and resistance to inorganic scaling were all affected by the amount of Fe(III)-TA-MoS 2 loading. The MoS 2 -3.0 NFM exhibited the highest roughness and uniformity (Ra: 111.25 ± 3.30 nm), along with enhanced hydrophilicity and the smallest pore size (0.39 nm) among the Fe(III)-TA-MoS 2 NFMs. These characteristics contributed to a high water permeance of 20.19 LMH/bar. Sulfate rejection decreased notably with increasing amine content, while calcium ion rejection showed a slight decline as surface electronegativity increased. Notably, the pore size of the Fe(III)-TA-MoS 2 NFMs remained largely unchanged at low and moderate loadings. Although the MoS 2 -4.5 NFM exhibited even higher water permeance and electronegativity, its surface structure underwent significant changes, leading to inferior performance in terms of scaling resistance and natural organic matter removal compared to the MoS 2 -3.0 membrane. Overall, this study provides a novel strategy for enhance both water permeability and anti-scaling performance of NFMs, offering potential cost reductions for drinking water treatment applications.
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FITOTERAPIA
·
2024
·
IF 2.5
Yungang Tian,Jingjing Fan,Rongshen Wang,Rui Ding,Chunxue Zhao,Jia Xu,Qingyun Zhang,Tiegui Nan,Xianchan Li,Min Ye
Five previously undescribed protopanaxatriol-type saponins, notoginsenosides Ta-Te ( 1 – 5 ), together with eighteen known triterpenoid saponins ( 6 – 23 ) were isolated from the roots of Panax notoginseng . The structures of new compounds were determined by HRESIMS and NMR spectroscopic analyses and chemical methods. Compounds 1 and 2 were the first examples of ginsenosides featuring a 6-deoxy- β - d -glucose moiety from Panax species. Compounds 1 – 4 , 7 , 10 , 12 , 21 – 22 showed protective effects on L02 cells against the injury of acetaminophen (APAP). Among them, notoginsenoside R1 ( 12 ), ginsenoside Rg1 ( 21 ), and ginsenoside Re ( 22 ) were the most potent ones, with cell viabilities >80%. Moreover, compounds 12 and 22 remarkably alleviated APAP-induced liver injury in mice. These saponins are potential hepatoprotective agents.
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JOURNAL OF MEMBRANE SCIENCE
·
2025
·
IF 9
Shuyi Xu,Ruiqi Zheng,Zhiying Li,Xin Tong,Xin Yu,Yangying Zhao
In response to emerging environmental challenges and the growing demand for resource recycling, nanofiltration (NF) technology has evolved from primarily targeting solute/solvent separation to enabling solute/solute separation. This includes the effective recovery of phosphates while simultaneously rejecting emerging micropollutants (EMPs), ensuring the safe reuse of nutrients. Among the key mechanisms influencing solute rejection, steric exclusion and Donnan effects have garnered considerable attention, driving the rapid development of electroneutral or low-charge membranes. In contrast, dielectric effects have received limited scrutiny. This study investigates the interplay between ion partitioning mechanisms and the influence of feedwater pH on the rejection of solute species, including phosphate ions and EMPs, by preparing electroneutral membranes with varying pore sizes and isoelectric points spanning different pH ranges. Experimental data were fitted using the Donnan-Steric Pore Model with Dielectric Exclusion (DSPM-DE) and the Steric, Electric and Dielectric Exclusion (SEDE) models, enabling a comprehensive examination of how membrane pore size, charge characteristics, and ion properties influence ion partitioning and transmembrane processes. The SEDE model, which integrates both the Born effect and image forces within the dielectric effects framework, outperformed the DSPM-DE model in accurately predicting ion rejection, making it well-suited for designing and optimizing NF membranes in scenarios that require selective separation of multivalent ions from other solutes. These findings underscore the previously overlooked importance of dielectric exclusion, offering valuable insights for the future development of customized NF membranes with enhanced selectivity for nutrient recovery and broader applications in environmental and industrial water treatment.
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Foods
·
2025
·Vol. 14
·期 23
·4126
·
IF 5.1
Yuemou Zhao,Wenyu Liang,Huaqun Chen,Jinwen Huang,Longyan Zhao,Qingxia Yuan
Heteropolysaccharides, the principal bioactive constituents of the esteemed medicinal foodTremella aurantialba, remain poorly understood in both structure and function. Herein, we describe a novel heteropolysaccharide, designated TAP-2a, isolated from the fruiting bodies ofT. aurantialbavia multi-step column chromatography. With a molecular weight of 16.95 kDa, TAP-2a is dominated by the pyranose forms of ᴅ-galactose (ᴅ-Galp), ᴅ-glucose (ᴅ-Glcp) and ᴅ-mannose (ᴅ-Manp), accompanied by minor proportions of ᴅ-xylose (ᴅ-Xylp), ʟ-fucose (ʟ-Fucp) and glucuronic acid. Methylation-GC-MS and exhaustive 1D/2D NMR analyses revealed a backbone assembled from →6)-α-Galp-(1→, →6)-β-Glcp-(1→, and →3)-α-Manp-(1→residues, branched at →2,6)-β-Galp-(1→, →3,6)-α-Galp-(1→, and →2,3)-α-Manp-(1→residues, and terminated by β-Glcp-(1→, α-Fucp-(1→, and β-Xylp-(1→. This intricate glycosidic architecture generates an exceptionally complex mannoglucogalactan in which a Gal→Man domain is substituted atO-3 of Gal by t-β-Glcpside chains and atO-2 of Man by t-α-Fucpstubs; additionally, a discrete fragment comprising t-β-Glcp-(1→3)-β-Glcp-(1→ was identified, along with a minor branch in which t-β-Xylpis attached toO-2 of a mannose residue. Functionally, TAP-2a proved to be a potent immunomodulator, markedly enhancing the secretion of nitric oxide, interleukin-1β, interleukin-6 and tumour necrosis factor-α while concurrently up-regulating the corresponding mRNA transcripts and augmenting phagocytic capacity. These findings establish the highly elaborate heteropolysaccharides ofT. aurantialbaas powerful immunomodulators that underpin the fungus’s renowned medicinal efficacy.
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Polymers
·
2025
·Vol. 17
·期 8
·1029
·
IF 4.9
Caiyun Liu,Shuzhen Ni,Zhaojiang Wang,Yingjuan Fu,Menghua Qin,Yongchao Zhang
During the conventional biomass fractionation, the degradation and dissolution of lignin and hemicellulose result in a complex extract which remains very challenging for the thorough separation and purification of a wide variety of fractionated products, limiting their further utilization. Herein, we proposed a facile and efficient strategy for fractionating biomass and simultaneously in situ converting of both lignin and hemicellulose into single products using a formic acid–phloroglucinol system. The introduced phloroglucinol could react with lignin fragments and hemicellulose-derived products, and the generated intermediate product from hemicellulose can be further condensed with lignin fragments, finally forming single lignin-based functional biopolymers containing heterocyclic structures. Only small amounts of hemicellulosic derivatives, such as oligosaccharides, monosaccharides, furfural, and 5-HMF, were detected in the extracted solution, indicating a highly directional and effective in situ conversion process of hemicellulose. The constructed specific structures on fabric surfaces by using the chelation between lignin-based functional biopolymers and metal ions achieved the preparation of functional fabrics with stable hydrophobicity. The dynamic contact angle of water droplets on the surface of prepared fabric only decreased from 122° to 116.8° over 30 min. This work strategy provides an ideal route to maximize the utilization of both lignin and hemicellulose without involving complex separation and purification procedures. This strategy is the first demonstration of using the targeted fractionation system to achieve the simultaneous conversion of hemicellulose and lignin into single functional biopolymers directly from lignocellulosic biomass.
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