Scientific African
·
2020
·
IF 0
Sujan Khadka,Jean Bosco Nshimiyimana,Piao Zou,Niranjan Koirala,Li Xiong
Environmental pollution due to di-ethyl phthalate (DEP) is one of the major concerns all over the world. This is probably the first study regarding the biodegradation of DEP by newly isolated Pseudomonas juntendi strain CCNU-SK1, P. putida strain CCNU-SK2 and P. nitritireducens strain CCNU-SK3. Bacterial strains were isolated by morphological, biochemical, physiological and 16S rDNA gene sequencing techniques. The biodegradation of DEP was quantified, and degradation kinetics were studied by using high-performance liquid chromatography. The optimum growth of bacteria for strain CCNU-SK1 was obtained at 28 °C, pH 8, DEP (500 mg/L), NaCl (1.5%), half-life (10.19 h); in case of strain CCNU-SK2: 33 °C, pH 7, DEP (400 mg/L), NaCl (1.5%), half-life (13.86 h) and for strain CCNU-SK3: 33 °C, pH 7, DEP (500 mg/L), NaCl (1.75%), half-life (12.6 h). Strain CCNU-SK1 with lowest half-life period was the most efficient bacteria for biodegradation of DEP compared to other isolated strains. More than 99% of all DEP were biodegraded within the 4 days and the complete utilization of (50 mg/L) DEP occurred within 24 h by all strains. All of them were capable of metabolizing not only DEP but also other phthalate esters (PAEs) such as DMP (Di-methyl phthalate), DBP (Di-butyl phthalate), DOP (Di-octyl phthalate), BBP (Benzyl butyl phthalate), DEHP (Di-2-ethylhexyl phthalate) and PA (Phthalic acid), making them the promising agents for the bioremediation in the PAEs contaminated sites.
研究领域:
环境科学
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微生物学
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生物技术
·
化学工程
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产品关联
DOI
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Yu-Xi Feng,Xin Chen,Yan-Wen Li,Hai-Ming Zhao,Lei Xiang,Hui Li,Quan-Ying Cai,Nai-Xian Feng,Ce-Hui Mo,Ming-Hung Wong
This study established a high-efficiency fluorescence quenching approach for the in situ visualization and modeling of the spatial distribution of xylanase, β-glucosidase, and phosphatase activities in plant leaves under pollution stress (namely, the leaf zymography technique, LZT). In the LZT, a membrane saturated with an enzyme-specific fluorescent substrate on the leaf surface was incubated and the fluorescence image generated on the membrane under ultraviolet light was recorded. An image-based modeling method for restoring the morphological traits of the true image by reducing noise was developed to ensure the accurate estimation of enzyme activities. The LZT could simultaneously measure 48 samples within 2 h, with good reproducibility. The results obtained by the LZT were comparable to those obtained by a conventional biochemical analysis method and presented low-cost and convenient advantages. This paper explains the theoretical basis required to investigate the realistic application of the LZT for assessing ecotoxicity in large-scale monitoring.
研究领域:
植物科学
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环境科学
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生物技术
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生态毒理学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Fangyuan Hou,Qingyun Chang,Nana Wan,Jie Li,Xiaohuan Zang,Shuaihua Zhang,Chun Wang,Zhi Wang
A novel porphyrin-based conjugated microporous polymer (PCMP) with microporous structure and nitrogen-rich pyrrole building blocks was synthesized. The PCMP was used as a coating material to prepare solid-phase microextraction (SPME) fibers by sol–gel technique. Due to the toxicity of the phthalate esters (PAEs) and the necessity for their sensitive determinations in some food samples, the SPME fiber was investigated for the extraction of eleven PAEs from six different children's milk beverages prior to their detection by gas chromatography-mass spectrometry. Under the optimal conditions, the linear response range for the PAEs was in the range from 0.03 to 200 µg L −1 and the limits of detection (S/N = 3) for the analytes were 0.01–3.00 μg L −1 . The method recoveries for the PAEs were between 80% and 120%, with the relative standard deviations varying from 1.3% to 9.8%. The method was successfully applied for the determination of PAEs in children's milk beverages.
研究领域:
分析化学
·
材料科学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2022
·
IF 9.988
Xuejun Wang,Hao Wu,Chuhan Dai,Xiaoyu Wang,Lvjing Wang,Jianming Xu,Zhenmei Lu
Co-contamination of organic pollutants and heavy metals is universal in the natural environment. Dibutyl phthalate (DBP), a typical plasticizer , frequently coexists with cadmium (Cd) in nature. However, little attention has been given to the impacts of co-contamination by DBP and Cd on microbial communities or the responses of microbes. To address this, a microcosm experiment was conducted by supplying the exogenous DBP-degrading bacterium Glutamicibacter nicotianae ZM05 to investigate the interplay among DBP-Cd co-contamination, the exogenous DBP-degrading bacterium G. nicotianae ZM05, and indigenous microorganisms . To adapt to co-contamination stress, microbial communities adjust their diversity, interactions, and functions. The stability of the microbial community decreased under co-contamination, as evidenced by lower diversity, simpler network, and fewer ecological niches . Microbial interactions were strengthened, as evidenced by enriched pathways related to microbial communications. Meanwhile, interactions between microorganisms enhanced the environmental fitness of the exogenous DBP-degrading bacterium ZM05. Based on co-occurrence network prediction and coculture experiments, metabolic interactions between the non-DBP-degrading bacterium Cupriavidus metallidurans ZM16 and ZM05 were proven. Strain ZM16 utilized protocatechuic acid, a DBP downstream metabolite, to relieve acid inhibition and adsorbed Cd to relieve toxic stress. These findings help to explain the responses of bacterial and fungal communities to DBP-Cd co-contamination and provide new insights for the construction of degrading consortia for bioremediation.
研究领域:
环境微生物学
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生物修复
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生态毒理学
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微生物生态学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Ning Zhang,Dan Li,Mengyao Mu,Minghua Lu
Increasing the stability of materials and affinity to the target has always been the pursuit. In this paper, the strategy of partially fluorinated metal–organic framework materials (MOFs) was introduced into the field of sample preparation for the first time, and the potential of this method to prepare materials with improved stability and extraction performance against environmental pollutants was verified. The UiO-66-(NH 2 ) 0.58 (4F) 0.42 coating was successfully prepared on carboxyl functionalized stainless steel fiber through covalent bonding method. Owing to the introduction of F element, the UiO-66-(NH 2 ) 0.58 (4F) 0.42 coating exhibited enhanced metal node acidity, hydrophobicity and stability, thus its extraction performance for phthalates (PAEs) was higher than that of pure UiO-66-NH 2 and commercial fiber coatings. The UiO-66-(NH 2 ) 0.58 (4F) 0.42 -coated fiber was coupled with GC–MS to detect PAEs, and the wide linear range (0.03–1000 ng/L), good correlation coefficients (0.9732–0.9998) and low detection limit (0.0039–0.016 ng/L) were obtained. The established method had also been successfully applied to detection of trace PAEs in plastic bags packed milk and bottled liquor with satisfactory recoveries (83.7 ± 5.1–118.1 ± 4.1 %). This research provides a new path for the preparation of environmental pollutant enrichment and removel materials with good performance.
研究领域:
材料科学
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环境科学
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分析化学
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Nai-Xian Feng,Fei Zhang,Yunchang Xie,Hui Bin,Lei Xiang,Yan-Wen Li,Fantao Zhang,Yunhong Huang,Hai-Ming Zhao,Quan-Ying Cai,Ce-Hui Mo,Qing X. Li
Phthalates (PAEs) are ubiquitous in soils and food products and thus pose a high risk to human health. Herein, genome mining revealed a great diversity of bacteria with PAEs-degrading potential. Mining of the genome of Raoultella ornithinolytica XF201, a novel strain isolated from Dongxiang wild rice rhizosphere, revealed the presence of two silenced tandem genes pcdGH (encoding protocatechuate 3,4-dioxygenase, 3,4-PCD), key aromatic ring-cleaving genes in PAEs biodegradation. Ribosome engineering was successfully utilized to activate the expression of pcdGH genes to produce 3,4-PCD in the mutant XF201-G2U5. The mutant XF201-G2U5 showed high 3,4-PCD activity and could remove 94.5 % of di-n butyl phthalate (DBP) in 72 h. The degradation kinetics obeyed the first-order kinetic model. Strain XF201-G2U5 could also degrade the other PAEs and the main intermediate metabolites, ultimately leading to tricarboxylic acid cycle. Therefore, this strategy facilitates novel bacterial resources discovery for bioremediation of PAEs and other emerging contaminants.
研究领域:
环境微生物学
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生物修复
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合成生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2022
·
IF 6.911
Xuecheng Zhang,Jun Gao,Qinghua Chu,Haixia Lyu,Zenghong Xie
A novel hybrid material (ZIF-7-NH 2 @MIP) combining high adsorption capacity of amino-functional zeolitic imidazolate framework and specific recognition ability of dual-template molecular imprinted polymer was synthesized. The prepared ZIF-7-NH 2 @MIP was characterized by a range of instrumental techniques and combined with high performance liquid chromatography for the detection of four phthalate esters. The limits of detection of the four PAEs were below the maximum residue limit of GB 3838-2002 in China, ranging from 0.04 to 0.08 μg L −1 . The recoveries of the three real samples of mineral water , carbonated drinks and pulpy juices spiked at 10 μg L −1 were 88.68–98.58% and the relative standard deviations was 1.19–3.40%. The above results showed that the obtained absorbent exhibited many advantages for extracting analytes in aqueous solution especially complex environment due to the combination of the superior hydrothermal and large specific surface area of the amino-functionalized ZIF-7-NH 2 and the specific identification of the dual-template molecular imprinted polymer.
研究领域:
分析化学
·
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2021
·Vol. 14
·期 11
·2375-2385
·
IF 3.366
Zhao Yuanli,Zhu Zhenbao,Liu Jianghua,Liu Jichao,Li Guoliang
Phthalate esters (PAEs) are prone to migrate from the plastic containers into oil during production and storage process. Owing to the trace amount of PAEs and complex sample matrix, it is of great importance to explore a facile and sensitive way to extract and detect PAEs from oil samples. In this work, magnetic covalent organic framework nanospheres (Fe 3 O 4 @COF(TbBD)) were fabricated by the aldehyde-amine condensation between benzidine (BD) and 1,3,5-triformylbenzene (Tb) on the surface of Fe 3 O 4 nanoparticles with the help of ultrasonic treatment. The new type nanomaterials were firstly used to the magnetic solid-phase extraction (MSPE) of seven PAEs in edible vegetable oils prior to high performance liquid chromatography coupled with a diode array detector (HPLC–DAD). In order to achieve the best extraction efficiency, several key parameters were optimized. Under optimal conditions, the proposed Fe 3 O 4 @COF(TbBD)-MSPE-HPLC–DAD method possessed high sensitivity (0.55–0.95 µg/kg for LODs and 1.80–3.10 µg/kg for LOQs), good linearity ( R 2 ≥ 0.9983), and satisfactory recoveries (80.2–102.9%). The RSDs for intra-day was less than 6.1%, and inter-day was less than 7.9%. This work not only proposed a rapid and reliable approach for determining PAEs in edible vegetable oils but also broadened the application of magnetic COFs in food determination.
研究领域:
食品科学
·
分析化学
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产品关联
DOI
高影响因子
Journal of Energy Chemistry
·
2023
·
IF 13.1
Jiaying Xie,Sibo Qiao,Yuyang Wang,Jiefei Sui,Lixia Bao,He Zhou,Tianshi Li,Jiliang Wang
The development of flame retardant or nonflammable electrolytes is the key to improve the safety of lithium batteries, owing to inflammable organic solvents and polymer matrix in common liquid and polymer electrolytes regarded as the main cause of battery fire. Herein, a series of solid-state polyphosphate oligomers (SPPO) as a three-in-one electrolyte that integrated the roles of lithium salt, dissociation matrix, and flame retardant were synthesized. The well-designed SPPO electrolytes showed an optimal ionic conductivity of 5.5 × 10 −4 S cm −1 at 30 °C, an acceptable electrochemical window up to 4.0 V vs. Li/Li + , and lithium ion transference number of 0.547. Stable Li-ion stripping/plating behavior for 500 h of charge–discharge cycles without internal short-circuit in a Li|SPPO|Li cell was confirmed, together with outstanding interface compatibility between the SPPO electrolyte and lithium foil. The optimal Li|SPPO|LiFePO 4 cell presented good reversible discharge capacity of 149.4 mA h g −1 at 0.1 C and Coulombic efficiency of 96.4% after 120 cycles. More importantly, the prepared SPPO cannot be ignited by the lighter fire and show a limited-oxygen-index value as high as 35.5%, indicating splendid nonflammable nature. The SPPO could be a promising candidate as a three-in-one solid-state electrolyte for the improved safety of rechargeable lithium batteries.
研究领域:
材料科学
·
能源存储
✓ 已验证
产品关联
DOI
高影响因子
Food Analytical Methods
·
2023
·Vol. 16
·期 4
·689-698
·
IF 2.9
Chu Qiqi,Wu Xinze,Chen Huaixia,Dang Xueping,Liu Xiaolan
A Cu 7 Zn 2 cluster functionalized copolymer monolithic column was prepared and applied as the adsorbent of pipette tip micro-solid phase microextraction of eight phthalate esters (PAEs). The physical morphology, chemical composition, thermal stability, and specific surface area of the monolithic column were characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy, thermogravimetric analysis, and N 2 adsorption and desorption experiment. Cu 7 Zn 2 cluster acted as the adsorption site effectively improved the adsorption selectivity and capacity of the copolymer monolith for PAEs. The adsorption mechanism of Cu 7 Zn 2 copolymer monolith for 8 PAEs was deduced to be mainly the H-bond interaction. And, the chemical doping and copolymerization of Cu 7 Zn 2 cluster made the composite monolithic column more stable with a long service life. Under the optimized extraction conditions, a micro-solid phase extraction-high performance liquid chromatography method with ultraviolet detector (μ-SPE-HPLC-UV) was developed for the determination of 8 PAEs in edible oil. Wide linear ranges of 0.5–1000 μg/kg with correlation coefficients ( R 2 ) above 0.996 were obtained. The limits of detection (LODs) were in the range of 0.1–0.9 μg/kg. The proposed method was applied for the detection of 8 PAEs in edible oil with satisfactory recoveries between 83.5~100.2%, and the relative standard deviations were less than 8.1%. The results indicated that the Cu 7 Zn 2 functionalized copolymerized monolithic column was an ideal enrichment medium for the detection of PAEs in complex samples.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Li Lin,Sheng Zhang,Lei Dong,Yueqi Cao,Wei Zhang,Xiong Pan,Yi Li,Chi Zhang,Jingxiang Tao,Di Jia,John Crittenden
Flood discharge atomization is a prevalent hydraulics phenomenon in reservoir scheduling operations, however, its effect on the migration and transformation behavior of pollutants has not been examined. In this study, the behaviors and mechanisms of the direct photodegradation of dibutyl phthalate (DBP) in atomized water and the indirect photodegradation of DBP in the presence of ferric ions and nitrate were investigated. The results showed that the photodegradation rate of DBP was accelerated under atomization conditions by sunlight irradiation. The photodegradation efficiency of DBP in the presence of ferric ions and nitrate under atomization conditions was increased by 2.20 times and 1.82 times compared with no-atomization conditions, respectively. The quencher experiments indicated that the main active species for DBP photodegradation in the presence of ferric ions were hydroxyl radicals (·OH) and superoxide radicals (·O 2 − ) with atomization, while the main active species in the presence of nitrate were ·OH, ·O 2 − and electrons (e − ). In addition, the differences were found in the photodegradation products and pathways of DBP between with and without atomization treatment. In the presence of ferric ions, the benzene ring of DBP was opened to produce fumaric acid, while phthalic acid bis(4-hydroxybutyl) ester was produced in the presence of nitrate under atomization conditions. The results of this study provide a scientific basis for assessing the effect of water conservancy projects on the migration and transformation behaviors of pollutants, which is of great theoretical significance and scientific value.
研究领域:
环境科学
·
水污染控制
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光化学反应
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产品关联
DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2023
·
IF 4.3
Yuan Liang,Qiuyan Jiang,Yiyao Gong,Yifan Yu,Haoyang Zou,Jingqi Zhao,Tiehua Zhang,Jie Zhang
As a promiscuous xenobiotic receptor, pregnane X receptor (PXR) has been confirmed to participate in numerous physiological process. In addition to the conventional estrogen/androgen receptor, PXR also serves as an alternative target for environmental chemical contaminants. In this work, the PXR-mediated endocrine disrupting effects of typical food contaminants were explored. Firstly, the time-resolved fluorescence resonance energy transfer assays confirmed the PXR binding affinities of 2,2′,4,4′,5,5′-hexachlorobiphenyl, bis(2-ethylhexyl) phthalate , dibutyl phthalate , chlorpyrifos , bisphenol A , and zearalenone , with IC50 values ranging from 1.88 to 4284.00 nM. Then their PXR agonist activities were assessed by PXR-mediated CYP3A4 reporter gene assays. Subsequently, the regulation of gene expressions of PXR and its targets CYP3A4 , UGT1A1 , and MDR1 by these compounds was further investigated. Intriguingly, all the tested compounds interfered with these gene expressions, confirming their endocrine disrupting effects via PXR-mediated signaling. The compound-PXR-LBD binding interactions were explored by molecular docking and molecular dynamics simulations to unravel the structural basis of their PXR binding capacities. The weak intermolecular interactions are key players in stabilizing these compound-PXR-LBD complexes. During the simulation process, 2,2′,4,4′,5,5′-hexachlorobiphenyl remained stable while the other 5 compounds underwent relatively severe disturbances. In conclusion, these food contaminants might exhibit endocrine disrupting effects via PXR.
研究领域:
毒理学
·
分子生物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）