JOURNAL OF MATERIALS SCIENCE
·
2014
·Vol. 49
·期 20
·6944-6951
·
IF 2.305
Wang Yongjuan,Zhou Yuming,Zhang Tao,He Man,Bu Xiaohai
The biomolecule–inorganic hybrid materials are potential candidates for low infrared emissivity materials due to the combined functionalities of the biomolecules and the inorganic components. In this study, we describe a facile approach to fabricate DNA/layered double hydroxides (LDHs) nanohybrid by coassembly of exfoliated Ni–In LDHs nanosheets with DNA. In the assembly progress, exfoliated LDHs nanosheets and DNA with different initial DNA/LDHs mass ratio were mixed together, and the DNA intercalated LDHs nanohybrid was obtained only when the input DNA/LDHs mass ratio is ≤0.3 mg/mg. The structure and morphology of as-fabricated hybrid materials were characterized by X-ray diffraction (XRD), UV–Vis absorption spectra, Fourier transform infrared (FT-IR) spectra, thermogravimetry (TG), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The results indicate that the reassembled product has layered structures incorporated with DNA biomolecule. The nanohybrid with expanding LDHs structure of d
003 = 2.6 nm confirms the intercalation of DNA molecules into the LDHs interlayer. The helix chain oriented parallel to the basal plane of host layer forming ordered sandwich structure. The infrared emissivity value of the DNA/LDHs nanohybrid was then investigated. It was found that the infrared emissivity values were significantly reduced by coassembly of exfoliated LDHs nanosheets with DNA, which may be attributed to the synergistic effect and interfacial interaction between the DNA biomolecule and inorganic LDHs layers reinforced by the construction of ordered sandwich structure.
研究领域:
材料科学
·
生物化学
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DOI
高影响因子
ACS Applied Nano Materials
·
2022
·
IF 6.14
Jing-Yuan Ma,Kai-Xuan Wang,Ming Ma,Yu Zhang
As an emerging storage medium, DNA is a crucial carrier for future data storage. DNA has a better storage density and a longer storage life than conventional storage media. However, DNA’s key limitations as a storage medium are the precision of information reading and storage stability. Herein, we designed and successfully developed a switchable DNA storage nanocomposite structure (MM-DNA/DNA’-SiO2). The composite structure employs magnetic microspheres (MMs) based on Fe3O4 nanoparticles as the DNA carrier for storing information (MM-DNA) and silicon dioxide nanoparticles modified with complementary strands as the protective outer layer (DNA’-SiO2), and its switchability and DNA sequence reading accuracy were both validated in this study. This method provides a novel nanostructure medium and technical proposal for efficient DNA storage with potential application value.
研究领域:
纳米技术
·
生物技术
·
数据存储
·
材料科学
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产品关联
DOI
高影响因子
Journal of Biomedical Nanotechnology
·
2018
·Vol. 14
·期 1
·150-160
·
IF 5.068
Li Taotao,Yi Huan,Liu Yuan,Wang Zunliang,Liu Shiquan,He Nongyue,Liu Hongna,Deng Yan
DNA strands have been used as templates to form different sized silver nanoclusters. Although DNA templated silver nanoclusters (NCs) probes which show outstanding fluorescence properties have been widely applied in chemical sensing and cellular imaging, synthesis of DNA template Au–Ag
bimetallic nanoclusters remains a challenge. A facile one-step synthesis method was thus developed in this study to form Au–Ag NCs using C4-ATAT-C4 as template. C4-ATAT-C4 acted as a stabilizer for preventing aggregation of the Au–Ag NCs. The obtained Au–Ag NCs stabilized
by C4-ATAT-C4 showed bright fluorescence and high stability. A series of experiments showed that temperature, citrate-citric acid buffer, sequence and concentration of DNA played an important role in the synthesis of fluorescent Au–Ag NCs. In addition, a ratiometric fluorescence probe
was designed through combining the nucleotide sequence (C4-ATAT-C4) and hybridization sequence in the presence of the double-strand-chelating dye Super SYBR Green (SG). The combination of prepared Au–Ag NCs, Super SG, and perfectly matched DNA emitted fluorescence at 500 and 590 nm,
respectively, when the composite was excited at 290 nm. A gastric cancer gene was also selectively detected by our developed ratiometric fluorescence probe.
研究领域:
生物化学
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纳米生物学
·
分子诊断
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产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2013
·
IF 3.777
Zhenning Xu,Mo Wang,Huanshun Yin,Shiyun Ai,Liang Wang,Junling Pang
A sensitive electrochemical method for detection of DNA methylation and assay of DNA methyltransferase (Dam) was investigated based on methylation-sensitive restriction endonuclease, DNA-Au bio bar code and enzymatic signal amplification. After the DNA probe was hybridized with complementary DNA sequence, and the obtained double-strand DNA (dsDNA) was further methylated by Dam MTase, it could be successively digested by Dpn I at the methylated sites. As a result, the bio bar code and streptavidin-horseradish peroxidase (S-HRP) could not be conjugated on the electrode surface through the specific interaction between biotin and S-HRP, where biotin was labeled at the DNA-Au bio bar code. Thus, only a low electrochemical reduction signal was obtained. However, the hybridized probe could hybrid with DNA-Au bio bar code, and then S-HRP could be conjugated on the electrode surface in the absence of Dam MTase and Dpn I, so a larger reduction current of enzymatic product was obtained. The change of the reduction current of enzymatic product was used to detect DNA methylation and Dam MTase activity. The detection limit of Dam MTase was 0.02 unit/mL (S/N = 3). Moreover, the inhibitions of mitomycin C, paclitaxel, chlorogenic acid and epicatechin were also investigated. Therefore, this method provided a sensitive platform for monitoring DNA methylation and DNA MTase activity.
研究领域:
生物化学
·
分子生物学
·
医学检测
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DOI
高影响因子
Journal of Pharmaceutical Analysis
·
2020
·
IF 2.673
Muhammad Abbas,Mirza Muhammad Faran Ashraf Baig,Yaliang Zhang,Yu-Shun Yang,Songyu Wu,Yiqiao Hu,Zhong-Chang Wang,Hai-Liang Zhu
The study aimed to achieve enhanced targeted cytotoxicity and cell-internalization of cisplatin-loaded deoxyribonucleic acid-nanothread (CPT-DNA-NT), mediated by scavenger receptors into HeLa cells. DNA-NT was developed with stiff-topology utilizing circular-scaffold to encapsulate CPT. Atomic force microscopy (AFM) characterization of the DNA-NT showed uniformity in the structure with a diameter of 50–150 nm and length of 300–600 nm. The successful fabrication of the DNA-NT was confirmed through native-polyacrylamide gel electrophoresis analysis, as large the molecular-weight (polymeric) DNA-NT did not split into constituting strands under applied current and voltage. The results of cell viability confirmed that blank DNA-NT had the least cytotoxicity at the highest concentration (512 nM) with a viability of 92% as evidence of its biocompatibility for drug delivery. MTT assay showed superior cytotoxicity of CPT-DNA-NT than that of the free CPT due to the depot release of CPT after DNA-NT internalization. The DNA-NT exhibited targeted cell internalizations with the controlled intracellular release of CPT (from DNA-NT), as illustrated in confocal images. Therefore, in vitro cytotoxicity assessment through flow cytometry showed enhanced apoptosis (72.7%) with CPT-DNA-NT (compared to free CPT; 64.4%). CPT-DNA-NT, being poly-anionic, showed enhanced endocytosis via scavenger receptors.
研究领域:
分子生物学
·
药物递送
·
癌症治疗
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产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2022
·
IF 5.545
Fan Zhang,Changqing Mao,Siyu Cao,Runchi Zhang,Yi Guo,Guifang Chen,Chang Feng
Bio-nanomicelles based on biomaterials such as nucleic acids, peptides, glycans, and lipids have developed rapidly in the field of bioanalysis. Although DNA and peptides have unique advantages, unfortunately, there are few bio-nanomicelles integrating DNA with peptides. Here, we designed a peptide-DNA hybrid bio-nanomicelle for the activity detection of caspase-3. The detection mechanism is based on caspase-3 specific recognition and cleavage of peptide substrates, which owns high sensitivity and selectivity. Under optimal conditions, the detection of caspase-3 activity can be achieved using our designed bio-nanomicelles and the detection limit is 0.72 nM. Furthermore, the proposed method was also successfully applied for the detection of caspase-3 in cell lysate samples after apoptosis-inducing.
研究领域:
生物分析
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纳米技术
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 28
·13228-13234
·
IF 3.3
Yuanyi Wu,Shihan Liu,Yumei Xiao,Qian Li,Qin Xiang,Xianqin Song,Shanglin Wu,Kaiyong Wang,Yi Yang
Accurate detection of DNA is critical for biomedicine and food safety. Layered double hydroxides (LDHs) are a class of two-dimensional layered materials that have received extensive attention in the fields of sensors, catalysis, and materials science. The application of LDHs in DNA sensing in turn represents a fascinating topic. In this study, we found that both 6-carboxyfluorescein (FAM)-modified single-stranded DNA (ssDNA) probes and probe/target double-stranded DNA (dsDNA) duplexes could be adsorbed by LDHs, which resulted in high fluorescence quenching (>95%) for the first time. Based on the extraordinarily high quenching efficiency of LDHs, we developed a fluorescent sensing platform for simple and highly efficient detection of DNA, using Ni/Al-LDHs as fluorescence quenchers. For the selective degradation of dsDNA, exonuclease III was integrated into the sensor. In the sensing platform, the fluorescence of the probes was rapidly quenched by Ni/Al-LDHs. In the presence of the target DNA, dsDNA was formed, which was subsequently selectively degraded by exonuclease III into short fragments, resulting in the maintenance of the fluorescence of FAM labels. The linear range was 10–400 nM. The detection limit was 1.38 nM. This sensor accomplished the detection of target DNA within 50 min. It provides a convenient, rapid and versatile sensing platform for DNA biomarkers.
研究领域:
生物医学工程
·
材料科学
·
分析化学
·
生物传感器
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DOI
高影响因子
CELL DEATH AND DIFFERENTIATION
·
2024
·
IF 13.7
Yang Jing,Wan Shi-yuan,Song Qiu-yi,Xie Yun-hao,Wan Jun,Zhou Yi-hao,Zhang Zi-tong,Xiao Yu-shuo,Li Xi,Chen Hong,Liu Xin-ran,Xu Li,You Hui-juan,Hu De-sheng,Petersen Robert B.,Zhang Yong-hui,Zheng Ling,Zhang Yu,Huang Kun
Upon genotoxic stresses, cells employ various DNA damage responses (DDRs), including DNA damage repair or apoptosis, to safeguard genome integrity. However, the determinants among different DDRs choices are largely unknown. Here, we report angiopoietin-like protein 8 (ANGPTL8), a secreted regulator of lipid metabolism, localizes to the nucleus and acts as a dynamic switch that directs DDRs towards apoptosis rather than DNA repair after genotoxin exposure. ANGPTL8 deficiency alleviates DNA damage and apoptosis in cells exposed to genotoxins, as well as in the liver or kidney of mice injured by hepatic ischemia/reperfusion or cisplatin treatment. Mechanistically, ANGPTL8 physically interacts with Poly (ADP-ribose) polymerase 1 (PARP1), in a PARylation-independent manner, and reduces the fluidity of PARP1-DNA condensates, thereby enhancing the pro-apoptotic accumulation of PARP1 and PAR chains on DNA lesions. However, the transcription of ANGPTL8 is gradually decreased following genotoxin treatment, partly due to downregulation of CCAAT enhancer binding protein alpha (CEBPA), presumably to avoid further cytotoxicity. Together, we provide new insights by which genotoxic stress induced DDRs are channeled to suicidal apoptosis to safeguard genome integrity.
研究领域:
癌症生物学
·
分子遗传学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
NANO
·
2020
·
IF 1.566
Zhikun Zhang,Xiaojie Ye,Qingqing Liu,Cuixia Hu,Jimmy Yun,Runjing Liu,Yumin Liu
Nucleic acid detection is becoming increasingly important in the diagnostics of genetic diseases for biological analysis. We herein propose gold nanoparticles as probe for colorimetric detection of nucleic acids with branched DNA nanostructures, which enables a novel and simple colorimetric biosensor. In our system, the target DNA specifically triggered two short-chain ssDNA probes to generate branched DNA nanostructures (Y-shape DNA), which prevent AuNPs from aggregation in aqueous NaCl solution. On the contrary, when the target DNA did not exist, gold nanoparticles were unstable and aggregated easily because there is no anti-aggregation function from Y-shape DNA. Sensor response was found to be proportional to the target DNA concentration from 5 to 100 nM, with detection limits determined as 5 nM. The developed platform is for colorimetric nucleic acid detection without enzymes, label and modification holds great promise for practical applications.
研究领域:
生物技术
·
纳米技术
·
分子生物学
·
分析化学
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产品关联
DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Qiang Zou,Xin Li,Tao Xue,Jia Zheng,Qi Su
A conceptually new class of DNA logic gates for Hg 2+ and Pb 2+ ion simultaneous detection has been constructed based on the activity of DNAzyme dependent on metal ions . For sensitive and specific detection of Hg 2+ and Pb 2+ ions, we demonstrate a surface-enhanced Raman scattering (SERS)-active platform by employing the oligonucleotide-functionalized and gold coated polystyrene microspheres (PSMPs). In this work, Hg 2+ and Pb 2+ were used as the input, and the SERS signals were the output. Hg 2+ ions were captured by a mismatched T-Hg-T bridge. Pb 2+ ions were bound to 8–17DNAzyme to break the cleavage substrate. The limit of detection (LOD) of Hg 2+ is 0.1 nmol L −1 and Pb 2+ is 1.0 nmol L −1 , which is far below the limit of 10.0 ppb (31 nmol L −1 ) and 10.0 ppm (3.02 × 10 4 nmol L −1 ) for drinking water stipulated by the World Health Organization. Additionally, this system has the potential to be ideal candidates for intelligent DNA calculator design applications. Furthermore, the designed strategy could be extended to other multiplex metal ions that can be combined with DNA molecules, and can be applied to many other fields such as environmental detection, toxin detection and liquid analysis.
研究领域:
分析化学
·
环境科学
·
生物化学
·
纳米技术
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 11
·1926-1932
·
IF 5.047
Fanyu Meng,Hua Chai,Xiaoyi Ma,Yuguo Tang,Peng Miao
Telomerase catalyzes the elongation of telomeres, which is closely associated with tumorigenesis. Therefore, development of reliable and convenient methods for telomerase activity analysis is critical for clinical diagnosis of cancers. However, most of the current probes for intracellular telomerase activity assay suffer from inevitable false-positive interferences. In this study, we have proposed a novel fluorescence resonance energy transfer (FRET)-based strategy for ultrasensitive monitoring intracellular telomerase activity with an amplification-free procedure. DNA nanoprobes constructed using a DNA tetrahedron and Flare DNA are designed to circumvent the problem of false-positive interference. Generally, telomerase catalyzes DNA extension and changes the configuration of the DNA nanoprobe, which finally leads to the increase of the distance between two labelled fluorophores. Based on the notably decreased FRET efficiency, a simple ratiometric sensor is established for the detection of telomerase activity at the single-cell level, which is much suitable for clinical applications. In addition, this method can be further applied in the detection of telomerase inhibitors, which is important for anti-cancer drug discovery.
研究领域:
分子生物学
·
生物技术
·
癌症研究
·
纳米技术
·
生物化学
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产品关联
DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2019
·
IF 3.743
Tianhua Liu,Ling Zuo,Dongqing Guo,Xinlou Chai,Jie Xu,Zhaorui Cui,Zhiyi Wang,Chunying Hou
Lung cancer is the leading cause of cancer-related deaths. Ginsenoside Rg3 is the main ingredient of Ginseng which is used to treat non-small cell lung cancer (NSCLC). It has been found to enhance the efficiency of chemotherapy thereby reducing its side effects. Previous studies found that ginsenoside Rg3 can reduce the occurrence of NSCLC by inducing DNA damage. Yet, its anti-DNA damaging effects and mechanisms in tumor cells are still not fully understood. This study explored the effect of ginsenoside Rg3 on DNA repair and VRK1/P53BP1 signaling pathway. Ginsenoside Rg3 treatment significantly decreased the incidence and invasionin a mouse model of lung cancer induced by urethane. The results of cell survival assay and single cell gel electrophoresis showed that ginsenoside Rg3 protected lung adenocarcinoma cells from DNA damage as well as inhibited the proliferation of tumor cells. Ginsenoside Rg3 increased the mRNA and protein expression of VRK1 in NSCLC cells as measured by RT-qPCR and western blot, respectively. These findings suggests that ginsenoside Rg3 regulates VRK1 signaling. Immunofluorescence assays showed that P53BP1 and VRK1 protein level increased, and the VRK1 protein translocated between the nuclei and cytoplasm. Finally, this conclusion was confirmed by the reverse validation in VRK1-knockdown cells. Taken together, these results show that ginsenoside Rg3 upregulate VRK1 expression and P53BP1 foci formation in response to DNA damage thereby inhibiting the tumorigenesis and viability of cancer cells. These findings reveal the role of Rg3 in lung cancer and provides therapeutic targets for developing new drugs in the prevention and treatment of lung cancer.
研究领域:
肿瘤学
·
分子生物学
·
药理学
·
癌症治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）