MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 6
·1-11
·
IF 5.3
Zhang Hui,Ji Yahui,You Nana,Hu Xiaoping,Chen Feifei,Wang Fei,Liu Gen
Ratiometric electrochemiluminescence (ECL) assays have attracted widespread attention in biosensing owing to their precise measurements by eliminating environmental interferences. In this work, g-C 3 N 4 , gold nanoparticles, CuO and luminol were integrated onto hollow carbon spheres (HCS) in sequence to fabricate potential-resolved ECL nanoprobes. The system consists of g-C 3 N 4 as cathode ECL emitters and luminol as anode ECL emitters. The ECL of g-C 3 N 4 is quenched by CuO due to the resonance energy transfer (RET). However, after adding dopamine (DA), the ECL signal is restored due to the redox reaction between CuO and DA. Meanwhile, there is a quenching effect between DA and luminol because DA interferes with the radical reaction process of luminol. Therefore, DA causes the reciprocal changes in cathodic ECL and anodic ECL. This phenomenon can be leveraged to create a ratiometric ECL signal, enabling the quantitative detection of DA. The developed ECL sensor exhibited a sensitive detection toward DA, performing a wide linearity in the range 5.0 × 10 –4 ~ 1.0 × 10 –9 M with a low detection limit of 2.3 × 10 –11 M (S/N = 3). Furthermore, this strategy exhibited a good practicality to detect DA in human urine, providing a promising strategy in ECL bioanalysis. Graphical abstract
研究领域:
分析化学
·
生物分析
·
生物传感器
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纳米技术
·
电化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 3
·1-8
·
IF 5.3
Yang Shuning,Tian Li,Song Guanying,Li Huiling,Li Chao,Wu Qian,Shan Xiangyu,Zhao Lun
An electrochemiluminescence (ECL) sensor for determining bisphenol A (BPA) was prepared based on titanium dioxide (TiO 2 ) and Co-MOF. TiO 2 is a co-reaction promoter that amplifies the ECL signal in the Ru(bpy) 3 2+ -trinpropylamine (TPrA) system. When the electrode is modified with Co-MOF the ECL signal is significantly enhanced. This is because Co-MOF can not only be used as a co-reaction accelerator but also as a carrier to adsorb more luminescent substances. Possible mechanisms for amplifying the original signal through the synergistic action of the two substances are investigated. The ECL strength decreases with increasing concentrations of BPA, and the amount of BPA can be determined by the change in ECL signal strength (Δ I ). Under optimal experimental conditions, the linear range of BPA was 2.0 × 10 −10 to 2.0 × 10 −5 M, with a determination limit of 6.7 × 10 −11 M (3 σ / m ). The relative standard deviation (RSD) of the signal for ten consecutive measurements was 1.5%. The sensor can be used to detect BPA in bottled samples with recoveries of 96 to 105%. Graphical
研究领域:
分析化学
·
环境科学
·
材料科学
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电化学
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传感器技术
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DOI
高影响因子
ANALYTICAL SCIENCES
·
2019
·Vol. 35
·期 8
·875-882
·
IF 1.618
Wang Xia,Zhai Suyan,Liu Chao,Wang Xiaoshu,Yang Ya,Tu Yifeng
An antibody-based immunotherapy for methamphetamine (MA) addictive treatment is has been drawing more and more attention in recent years. However, studies about methamphetamine antibody (anti-MA) immunodetections are rare, owing to the lack of immunogenicity of small molecule MA. This study provides a simple and effective approach to develop a convenient electrochemiluminescent (ECL) immunosensor for the testing of anti-MA. In short, the synthetic holoantigen of MA is immobilized on a homemade gold nanoparticles modified electrode as the sensing host for the specific recognition and detection of anti-MA. The research suggested, under optimal experimental conditions, the ECL intensity on resultant immunosensor has a wide-linear regression toward the anti-MA quantity within the range from 0.03 to 3.07 ng with a detection limit of 2.32 pg. It responded to the dosage of anti-MA in spiked blood samples with satisfactory recovery. According to the research, the developed sensor shows promise as a portable Anti-MA fast seized device which performs quickly and offers convenience, and will be helpful for forensic identification and clinical treatment.
研究领域:
分析化学
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生物医学工程
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法医学
·
药物成瘾研究
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DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Peijin Wang,Qian Wu,Caixia Wang,Xue Yang,Min Wei,Yuxin Pu,Mao Zhou,Mingxiao Zhang
In this work, Prussian blue nanocubes functionalized partial reduced graphene oxide (PBs-PrGO) was first employed as a new electrochemiluminescence (ECL) signal amplification strategy of perylenetetracarboxylic acid (PTCA), and the ECL signal of PBs-PrGO-PTCA composite presented 3-fold enhancement compared with PTCA. The Fe III and Fe II active site of Prussian blue nanocubes (PBs) could promote the reduction of K 2 S 2 O 8 to generate more sulfate radical anion (SO 4 − ), which enhanced the ECL signal of PTCA. The partial reduced graphene oxide (PrGO) not only used as the substrate for in-situ growing PBs, but also could assemble PTCA through π–π stacking. Besides, folic acid (FA) could effectively quench the ECL signal of PTCA. Based upon above, a rapid and effectual FA sensor was constructed. Under the optimum conditions, the ECL sensor exhibited an excellent linear relationship for FA determination with the limit of detection low as 0.719 nM. The constructed sensor applied in the determination of FA in tablets and human urine with satisfactory results, which could realize rapid and accurate FA analysis.
研究领域:
分析化学
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纳米材料
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生物传感器
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产品关联
DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Fen Ma,Yu Chen,Yinchang Zhu,Jiawei Liu
A new electrogenerated chemiluminescence (ECL) biosensor is fabricated for the determination of mental ion incorporating DNA three-way junction structure (DNA-TWJ). As a model system, Hg 2+ was chosen as an analyte. The ECL biosensor was fabricated by covalently coupling Hg 2+ special DNA-TWJ tagged with ruthenium (II) complex (Ru) (named TW/Ru-J1) to the surface of glassy carbon electrode that had been covalently modified with 4-aminobenzoic acid via electrochemical oxidations . Upon binding of Hg 2+ to the TW/Ru-J1, the confirmation of TW/Ru-J1 changed and induced Ru away from surface of electrode and thus led to a low ECL signal. The signal linearly decreases with the concentration of Hg 2+ in the range from 0.1 pM to 10 pM with a detection limit of 0.04 pM This study could be easily extended to various analytical platforms for the detection of many kinds of analytes or their interactions such as DNA/RNA, DNAzyme/target, aptamer/target, and antibody/antigen.
研究领域:
分析化学
·
环境科学
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生物化学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Xiaomin Fu,Yan Yang,Na Wang,Shihong Chen
Here, the cathodic electrochemiluminescence (ECL) behavior of 3,4,9,10-perylenetetracar-boxylic acid (PTCA) coreacted with hydrogen peroxide was firstly reported. The quenching effect of Fe-MIL-88 MOFs (iron based metal-organic framework) on the ECL of PTCA was found, and the ECL quenching mechanism was investigated, which was speculated to be via the resonance energy transfer from PTCA to Fe-MIL-88 MOFs and steric hindrance effect from Fe-MIL-88 MOFs. Combining with dual molecular recognition strategy and the quenching effect of Fe-MIL-88 MOFs on the ECL of PTCA-H 2 O 2 system, an “on-off” ECL sensor was designed to detect dopamine (DA) with a broad linear range (five orders of magnitude). The detection limit was low to 2.9 × 10 −13 M. The PTCA-H 2 O 2 system would provide a new ECL platform, especially for constructing enzyme based ECL sensor with H 2 O 2 as product in enzymatic reaction. Furthermore, the electrochemiluminescence resonance energy transfer between Fe-MIL-88 metal–organic framework and PTCA would expand a new application of Fe-MIL-88 MOFs in ECL field. The dual molecular recognition strategy ensured the selectivity of the sensor for DA detection.
研究领域:
分析化学
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材料科学
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电化学
·
传感器
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 3
·1-10
·
IF 6.232
Huang Bo,Liu Xing-Pei,Chen Jing-Shuai,Mao Chang-jie,Niu He-Lin,Jin Bao-Kang
An electrochemiluminescence (ECL) biosensor was fabricated for the evaluation of prostate specific antigen (PSA). The sensor was developed by successively modifying glassy carbon electrode (GCE) electrodes with CdS/Chito/g-C 3 N 4 nanocomposites and DNA1 was labeled at the 5′ end with thiol. The aptamer DNA was labeled at the 3′ end with a quencher ferrocene (Fc) was ligated to DNA1 by the principle of complementary base pairing. In the absence of PSA, the ECL intensity signal is effectively quenches through the energy transfer and photoexcitation electron transfer between CdS/Chito/g-C 3 N 4 emitter and quencher Fc. After incubation with target PSA, the aptamer DNA interacts with PSA and then moved away from the electrode surface together, which will recover the ECL intensity. Under the optimal conditions, the ECL intensity increases linearly with the logarithm of PSA concentration in the range of 1 pg·mL −1 to 100 ng·mL −1 , and the detection limit is 0.14 pg·mL −1 (S/ N = 3). The biosensor has been successfully applied to the determination of PSA in serum sample.
Schematic representation of the electrochemiluminescence sensor based on a CdS/chitosan/g-C 3 N 4 nanocomposite, which can be applied to the determination of prostate specific antigen in serum.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Fan Yang,JinBo Gong,Ming Li,Xiangyang Jiang,Jiawen Zhang,Meiyan Liao,Hanfei Zhang,Pier-Luc Tremblay,Tian Zhang
PCR-based techniques routinely employed for the detection of mutated linear DNA molecules, including circulating tumor DNA (ctDNA), require large nucleotide sections on both sides of the mutation for primer annealing. This means that DNA fragments with a mutation positioned closer to the extremities are unlikely to be detected. Thus, sensors capable of recognizing linear DNA with characteristic mutations closer to the ends would be advantageous over the state-of-the-art approaches. Here, an electrochemiluminescence-resonance energy transfer (ECL-RET) biosensor comprising capped CdS quantum dots and hairpin DNA probes labeled with Au nanoparticles was developed for the detection of epidermal growth factor receptor (EGFR) ctDNA carrying the critical T790M lung cancer mutation. The ECL-RET system detected different DNA molecules including single-stranded 18-nucleotides (nt) and 40-nt as well as double-stranded 100-nt with the single nucleotide polymorphism (SNP) coding for T790M located either in the middle or only 7 nt from one end. For all target DNA, the sensor’s limits of detection (LODs) were in the aM range, with excellent selectivity. It was the case of 100-nt target linear ctDNA fragments with LODs of 8.1 and 3.4 aM when the EGFR T790M SNP was either in the middle or at the end, respectively. These results show that ECL-RET systems can sense mutations in DNA fragments that would remain undetected by standard techniques.
研究领域:
生物传感器
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纳米技术
·
癌症研究
·
分子生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Jiajing Xu,Weiqiang Yang,Jiancong Ni,Qingxiang Wang,Zhenyu Lin
Electrochemiluminescence (ECL) arises from the collisional annihilation between the electro-excited luminophore and co-reactant. Most ECL-based bioassays require extensive free co-reactants that collide with luminophores randomly. Herein, the N,N-dimethylaniline (DMA) group was electro-grafted on the ITO electrode surface as the bottom co-reactant layer, and the vertically ordered mesoporous silica film (VMSF) was prepared as the upper layer to regulate the accessibility of Ru(bpy) 3 2+ . Thus, the dual-layer functionalized VMSF/N-ITO was newly built for the controllable ECL study. Relevant conditions, including the DMA electro-grafting, VMSF thickness, buffer concentration and pH value, were discussed to improve the ECL efficiency. Based on the VMSF/N-ITO platform, a homogeneous ECL bioassay was developed for miRNA-21 detection. The target nucleotide sequence induced the hybridization chain reaction (HCR) of probes H1 and H2 to absorb free Ru(bpy) 3 2+ as much as possible. Residual Ru(bpy) 3 2+ permeated the VMSF nanochannels to collide with the DMA co-reactant in producing an ECL response. There was a linear relationship between ∆ECL intensity and logarithmic concentration of miRNA-21 in the 1 fM ∼ 100 pM range, and the detection limit was 0.25 fM. The developed approach was probes immobilization-free, highly sensitive, and anti-interference, opening a new avenue for ECL-based bioassays.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
生物传感器
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Waner Hou,Xun Lei,Zhimin Liu,Xuanxuan Hao,Jintao Cheng,Yaxin Li,Leqian Hu,Peng Li
Herein, ZnCo bimetallic zeolite imidazole framework (ZnCo-ZIF) nanocubes were synthesized and used as 2e − oxygen reduction reaction (2e-ORR) electrocatalysts. ZnCo-ZIFs showed excellent ORR catalytic activity, which was benefit for producing reactive oxygen species (ROS) efficiently and enhancing the electrochemiluminescence (ECL) emission from luminol/H 2 O 2 system. The reaction mechanism investigation implied that ORR reaction could produce ROS, and the ECL response exhibited a positive correlation with the catalytic activity of ZnCo-ZIFs promoter. In addition, the electrocatalytic activity of different Zn/Co molar ratio (1/ 9, 1/5, 1 /1, 5/1 and 9/1) were investigated, the results showed that ZnCo-ZIF-IV with Zn/Co molar ratio of 5/1 displayed the highest ORR electrocatalytic performance, and the onset potential, H 2 O 2 production rate, the faraday efficiency were 0.67 V (vs. RHE), 67.17 % and 50.74 %, respectively. Finally, an ECL immunosensor based on the promoting effect of ZnCo-ZIF-IV nanocubes on the luminol/H 2 O 2 ECL system was proposed for the sensitive zearalenone assay. This strategy offers a new way to study the luminol-cathodic ECL system.
研究领域:
电化学
·
纳米材料
·
催化化学
·
分析化学
·
生物传感器
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产品关联
DOI
高影响因子
ANALYST
·
2019
·Vol. 144
·期 22
·6554-6560
·
IF 4.019
Liping Zhu,Jing Ye,Mengxia Yan,Qiuju Zhu,Xiurong Yang
In this study, a wavelength-resolved electrochemiluminescence resonance energy transfer (ECL-RET) ratiometric immunosensor from Au nanoparticle functionalized graphite-like carbon nitride nanosheets (Au-g-C3N4) to Au nanoclusters (Au NCs) has been constructed for the first time. At a working voltage of 0 to −1.2 V, Au-g-C3N4 showed a strong cathodic ECL emission with a peak at 460 nm, which overlapped well with the absorption spectra of Au NCs thus stimulating the fluorescence emission of Au NCs at 610 nm. Moreover, within this voltage range, the Au NCs showed no ECL signal; therefore, they would not interfere with the detection of the system. We used cardiac troponin I (cTnI) as an analytical model to construct a sandwich immunosensor based on the ECL-RET ratiometric strategy. By measuring the responses of the ECL460 nm/FL610 nm ratio at different cTnI concentrations, the sensitive detection of cTnI with a wide range of 50 fg mL−1 to 50 ng mL−1 and a low detection limit of 9.73 fg mL−1 can be achieved. This work enriches the wavelength-resolved ECL-RET system and provides an innovative reference for the development of more efficient and sensitive ECL-RET ratiometry.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
电化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Mei Zhang,Qianqian Kong,Jingcheng Huang,Yaodong Xiang,Guanjie Wang,Jie Han,Yemin Guo,Shancang Zhao,Xia Sun
Within this work, an electrochemiluminescence (ECL) aptasensor was constructed for the sensitive detection of diazinon . Primarily, AuNPs not only provided a platform for luminol fixation due to their favorable bioactivity and conductivity , but they facilitated the improvement of ECL signals. Furthermore, three-dimensional (3D) flower-like ZnONPs were obtained by hydrothermal synthesis , which could be an efficacious catalyst for the luminol-hydrogen peroxide (luminol-H 2 O 2 ) system, whereby the ECL signal of the sensor could be amplified. Given the large specific surface area of 3D flower-like ZnONPs, modification of positive charges on their surface could well immobilized the electronegative aptamer chains on the electrode surface , and when diazinon (DZN) was added, the aptamer combined with DZN to form a complex to hinder the ECL intensity, and the detection of DZN was achieved by the change of ECL signal intensity. After evaluated the experimental parameters, the detection limit of DZN was 0.0109 nM (S/N = 3), and the linear range was 0.0328–3.28 × 10 4 nM. The proposed ECL aptasensor had comparatively satisfactory detection limits, which provided a reasonable reference for other contaminant residues detection.
研究领域:
分析化学
·
环境科学
·
食品安全
·
纳米技术
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）