PLANT PATHOLOGY
·
2015
·Vol. 64
·期 5
·1041-1052
·
IF 2.121
W. Raza,J. Yuan,Y. C. Wu,F. U. Rajer,Q. Huang,S. Qirong
The aim of the present study was to investigate the effects of the phytotoxin fusaric acid (FA) on the biocontrol traits of two biocontrol strains Paenibacillus polymyxa WR-2 and SQR-21. The results showed that the growth of both WR-2 and SQR-21 decreased with increasing FA concentration, and at 70 and 80 μ g mL −1 FA, respectively, the strains were unable to grow. The biocontrol traits of both strains were negatively affected by FA concentration higher than 2·5 μ g mL −1 . However, at 2·5 μ g mL −1 FA, biofilm formation and root colonization were not affected and there was even a positive effect on the production of spores and hydrolytic enzymes (protease and β -l,3-glucanase). The production of fusaricidin-type antifungal compounds was increased with an increase in FA concentration up to 50 and 60 μ g mL −1 for WR-2 and SQR-21, respectively. The production of antifungal volatile organic compounds by WR-2 and SQR-21 was increased only at 2·5 μ g mL −1 FA. The effect of FA on the overall metabolic activity of WR-2 and SQR-21 was also determined. This study will help to understand the response of P. polymyxa strains to FA and will help to improve their biocontrol efficiency.
研究领域:
微生物学
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植物病理学
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农学
✓ 已验证
DOI
高影响因子
Food Analytical Methods
·
2018
·Vol. 11
·期 6
·1780-1788
·
IF 2.245
Zhao Zhiyong,Yang Xianli,Zhao Xiaoyan,Chen Lei,Bai Bing,Zhou Changyan,Wang Jianhua
Fusarium species are one of the common pathogenic fungi of vegetable diseases. Some Fusarium species produce toxic secondary metabolites, which have potential health risks for humans and animals. In the study, an ultra-performance liquid chromatography tandem mass spectrometry method (UPLC-MS/MS) was developed for the analysis of 13 emerging and traditional Fusarium toxins in pepper, tomato, cucumber, and potato. Samples were extracted by a modified quick, easy, cheap, effective, rugged, and safe method. Dispersive solid-phase extraction was employed for the removal of matrix interferences from the crude extracts of various vegetables. Matrix effect was compensated using matrix-matched calibration curves. The method was fully validated according to the European Commission Decision 2002/657/EC. For all the targeted analytes, good linearity ( R 2 ≥ 0.9823) was obtained. Recoveries were in the range of 62 and 114% in pepper, tomato, cucumber, and potato, with intra-day relative standard deviations lower than 14.3%. Quantitation limits of the developed method were between 0.25 and 20 μg/kg in four sample matrices. The method was successfully employed for the analysis of Fusarium toxins in vegetable samples.
研究领域:
食品科学
·
毒理学
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产品关联
DOI
高影响因子
JOURNAL OF PLANT PATHOLOGY
·
2022
·Vol. 104
·期 3
·979-992
·
IF 2.643
Mosa Mohamed A.,El-Abeid Sozan E.,Khalifa M. M. A.,Elsharouny T. H.,El-Baz Sahar M.,Ahmed Ayman Y.
Mesoporous silica nanoparticles (MSNs) can promote the solubility and absorption of pesticides by plants and are widely used as a delivery system to improve the efficacy of pesticide applications. In this study, MSNs with 20 nm particle size were produced. Additionally, a water-soluble chitosan (CS) derivative, here called N-(2-Hydroxyl) propyl-3- tri-methyl-ammonium CS chloride (HTCC) was produced and used to cap the outer surface of the MSNs preloaded with the pesticide fludioxonil (Flu). The HTCC coating layers resulted in a pesticide loading efficiency of 84% on the MSNs in comparison to a loading efficiency of 20% of uncoated particles. A comparative in vitro analysis indicated that Flu@MSNs 20nm -HTCC loaded with a 0.05 mg/L dose of fungicide had significant higher fungicidal activity than the same fungicide at 1 mg/L dose against F. oxysporum f. sp. radicis-lycopersici (FORL). Moreover, after an initial burst, MSNs 20nm -HTCC kept releasing Flu for 21 d, compared to an activity of 7 d associated with the direct release of Flu. Greenhouse data showed that 0.1 mg/L Flu applied through MSNs 20nm -HTCC is sufficient to reduce Fusarium crown and root rot disease severity to a value of less than 6% in tomato plants, without any noticeable phytotoxicity after 70 d. In comparison, 1.56 mL/L of the fungicide are required to reach a 27% disease severity level. Thus, we suggest that HTCC-decorated MSNs 20nm has a great potential as a nanodelivery systems for agrochemical applications. We also suggest that this work contributes to the notion that agro-nanotechnology is a powerful, environmentally-safe and cost-effective approach for a sustainable and long term protection of plants from disease.
研究领域:
植物病理学
·
纳米技术
·
农业科学
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产品关联
DOI
高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
·
2021
·Vol. 131
·期 4
·1890-1908
·
IF 3.772
Win T.T.,Bo B.,Malec P.,Fu P.
AimsThis study sought to utilize indigenous soil micro‐organisms to suppress wilt‐causing fungal pathogens of the banana.Methods and ResultsFungal pathogens were isolated from wilt‐affected rhizospheric soil, and potential antagonistic bacterial strains were isolated from healthy rhizospheric soil in the same area from which fungal pathogens were isolated. The antifungal activity of isolated micro‐organisms against fungal pathogens was studied both in vitro and in vivo against fungal pathogens. It was found that Fusarium oxysporum and Alternaria sp. were pathogenic, while Penicillium sp., Bacillus velezensis and Bacillus subtilis were antagonistic. Moreover, it was seen that B. velezensis, B. subtilis and Penicillium sp. inhibited the growth of the two fungal pathogens in both in vitro and in vivo experiments. Further investigation indicated that B. velezensis, B. subtilis and Penicillium sp. were able to produce enzymatic antifungal compounds (chitinase and β‐1,3‐glucanase). The spray application around rhizome revealed that a combination of Bacillus spp. and Penicillium sp. in greenhouse conditions gave the highest reduction in disease severity by up to 60% to both fungal pathogens among the treatments.ConclusionsBanana disease is seen to be induced not only by F. oxysporum but also by Alternaria sp. The isolated indigenous micro‐organisms can effectively control both the pathogens. The combination of isolated antagonistic micro‐organisms has thus demonstrated substantial potential for suppressing banana disease.Significance and Impact of the StudyAn antagonistic consortium isolated in this study has demonstrated remarkable potential for controlling fungal diseases caused by Fusarium sp. and Alternaria sp. Therefore, the use of indigenous microflora to improve disease suppression of banana plants against soil‐borne pathogens is a preferable approach.
研究领域:
微生物学
·
植物病理学
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农学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Fungi
·
2023
·Vol. 9
·期 1
·94
·
IF 4.7
Azizullah,Muhammad Noman,Yizhou Gao,Hui Wang,Xiaohui Xiong,Jiajing Wang,Dayong Li,Fengming Song
SUMOylation is an essential protein modification process that regulates numerous crucial cellular and biochemical processes in phytopathogenic fungi, and thus plays important roles in multiple biological functions. The present study characterizes the SUMOylation pathway components, including SMT3 (SUMO), AOS1 (an E1 enzyme), UBC9 (an E2 enzyme), and MMS21 (an E3 ligase), inFusarium oxysporumf. sp.niveum(Fon), the causative agent of watermelon Fusarium wilt, in terms of the phylogenetic relationship, gene/protein structures, and basic biological functions. The SUMOylation components FonSMT3, FonAOS1, FonUBC9, and FonMMS21 are predominantly located in the nucleus.FonSMT3,FonAOS1,FonUBC9, andFonMMS21are highly expressed in the germinating macroconidia, but their expression is downregulated gradually in infected watermelon roots with the disease progression. The disruption ofFonUBA2andFonSIZ1seems to be lethal inFon. The deletion mutant strains forFonSMT3,FonAOS1,FonUBC9, andFonMMS21are viable, but exhibit significant defects in vegetative growth, asexual reproduction, conidial morphology, spore germination, responses to metal ions and DNA-damaging agents, and apoptosis. The disruption ofFonSMT3,FonAOS1,FonUBC9, andFonMMS21enhances sensitivity to cell wall-perturbing agents, but confers tolerance to digestion by cell wall-degrading enzymes. Furthermore, the disruption ofFonSMT3,FonAOS1, andFonUBC9negatively regulates autophagy inFon. Overall, these results demonstrate that the SUMOylation pathway plays vital roles in regulating multiple basic biological processes inFon, and, thus, can serve as a potential target for developing a disease management approach to control Fusarium wilt in watermelon.Keywords:asexual reproduction;cell death;cell wall integrity;Fusarium oxysporumf. sp.niveum;mycelial growth;stress response;SUMOylation pathway;watermelon
研究领域:
植物病理学
·
分子生物学
·
真菌遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Yao Xiao,Zaizhi Liu,Huiyan Gu,Lei Yang,Tingting Liu,Hao Tian
In this study, α-amylase (α-A) and 2-octenylsuccinic anhydride (OSA)-modified Canna starch (Cs) were prepared and characterized as wall materials and encapsulated with methyleugenol (α-A-OSA-Cs-methyleugenol); their in vitro antifungal activity against Fusarium trichothecioides ( F. trichothecioides ) was also investigated. The encapsulation efficiency under optimal encapsulation conditions was 83.98%. The results of particle size, polydispersity index (PDI), zeta potential, electron scanning microscopy and Fourier transform infrared spectroscopy showed that the modified Cs had superior physicochemical properties; it was also demonstrated that methyleugenol successfully entered the pores of Cs. The in vitro release study showed that α-A-OSA-Cs could effectively reduce their volatility under different temperature environments. α-A-OSA-Cs have excellent performance as slow-release wall materials, and after encapsulation with methyleugenol, the inhibition ability of F. trichothecioides mycelium growth was dose-dependent and improved, extending the shelf life of potatoes, which has good commercial value in the field of slow-release preservatives.
研究领域:
食品科学
·
药学
·
微生物学
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植物病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2021
·
IF 5.275
Heng Guo,Qilin Wu,Dongni Chen,Minghua Jiang,Bin Chen,Yongjun Lu,Jing Li,Lan Liu,Senhua Chen
Under guidance of 1 H NMR, ten new polypropionate derivatives, decempyrones A–J ( 1 – 10 ) along with two known analogues ( 11 and 12 ), were isolated from the marine-derived fungus Fusarium decemcellulare SYSU-MS6716. The planar structures were elucidated on the basis of extensive spectroscopic analyses (1D and 2D NMR, and HR-ESIMS). The absolute configuration of the chiral centers in the side chain is a major obstacle for the structure identification of natural polypropionate derivatives. Herein, the J -based configurational analysis (JBCA), chemical degradation, geminal proton rule, and the modified Mosher’s method were adopted to fix their absolute configurations in the side chain. Compounds 3 and 10 exhibited potent anti-inflammatory activity by inhibiting the production of NO in RAW264.7 cells activated by lipopolysaccharide with IC 50 values 22.4 ± 1.8 and 21.7 ± 1.1 μM. In addition, compounds 3 and 10 displayed MptpA inhibitory activity with an IC 50 value of 19.2 ± 0.9 and 33.1 ± 2.9 µM. Structure-activity relationships of the polypropionate derivatives were discussed.
研究领域:
化学
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药理学
·
生物化学
·
海洋生物学
✓ 已验证
DOI
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Yunlei Zhou,Zhuangzhuang Wang,Huanshun Yin,Xiaoting Cui,Ying Tian,Zhen Qiao,Suo Wang,Runye Hu,Wenjing Lv,An Mao,Jun Wang
2D Ag/BiOCl/Bi 2 O 2 CO 3 S-scheme heterojunction was prepared with oxygen vacancy (OVs) via one-pot hydrothermal method. The XRD and XPS analysis indicated the synthesized sample contained Ag nanoparticles (AgNPs) instead of Ag ions. The SEM and HRTEM pictures showed that BiOCl/Bi 2 O 2 CO 3 nanosheets were modified with AgNPs. Compared with AgNPs, BiOCl, and Bi 2 O 2 CO 3 , Ag/BiOCl/Bi 2 O 2 CO 3 exhibited highly photocatalytic inactivation of pathogenic fungi ( Fusarium graminearum ) due to the wide light absorption range and S-scheme heterojunction structure, which improved the production and transfer of photogenerated carrier, and enhanced the separation of photogenerated e − /h + pairs. In addition, the improved photocatalytic disinfection against Fusarium graminearum of Ag/BiOCl/Bi 2 O 2 CO 3 was verified in Sedeveria Letizia plant . Furthermore, active species capture assay and ESR experiments disclosed the involvement of OVs, h + , ∙O 2 − , ∙OH, and – for Fusarium graminearum destruction during photocatalysis process. The S-scheme heterojunction was proved via oxygen vacancy, which was extensively beneficial to increase charge transmission and separation efficiency. Our work proposed Ag/BiOCl/Bi 2 O 2 CO 3 was an efficient and ecological fungicide to inactive Fusarium graminearum in vitro and vivo for crop disease .
研究领域:
材料科学
·
化学
·
环境科学
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农业科学
·
植物病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PLANT PATHOLOGY
·
2025
·
IF 1.9
Huang Zhixing,He Zhenrui,Ding Jiexin,Zhu Yiming,Zhou Erxun
Soybean root rot, caused by Fusarium neocosmosporiellum , poses a significant threat to soybean productivity. Although chemical fungicides are widely used, their environmental risk necessitate sustainable alternatives. This study proposes an integrated control strategy combining the biocontrol bacterium Pseudomonas aeruginosa E1 - 23 with prochloraz. Initial screening of seven candidate bacterial strains identified E1 - 23 as the most potent antagonist, exhibiting 64.1% inhibition against F. neocosmosporiellum in dual-culture assays. Concurrent fungicide evaluation revealed prochloraz as the optimal agent (EC 50 = 0.1646 μg/mL). Compatibility analysis demonstrated E1 - 23's robust tolerance to prochloraz concentrations at ≤ 50 μg/mL. In vitro combination of prochloraz (0.25 μg/mL) and E1 - 23 (1 × 10 6 CFU/mL) achieved 91.86% pathogen suppression, surpassing individual treatments by > 10% and theoretical additive effects. Greenhouse trials confirmed enhanced efficacy, with integrated application increasing disease control efficacy by 34.35%—significantly outperforming standalone bacterial (18.96%) or fungicide (20.52%) controls. The qRT-PCR analysis revealed that the combination application of E1 - 23 and prochloraz up-regulated the expression of some defense genes of soybean plants 5 days post-inoculation (dpi), but the induced plant disease resistance was low. This study highlights the potential of E1 - 23-prochloraz as an eco-friendly approach to mitigate soybean root rot while reducing fungicide dependency.
研究领域:
植物病理学
·
农业科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jiana Lin,Gongke Li,Yuling Hu,Qisheng Zhong
Fusarium mycotoxins are toxic secondary fungal metabolites and widely distributed in cereals. Herein, a nickel ferrite magnetic calix[4]arene-derived covalent organic framework (NiFe 2 O 4 @CX4-COF) was meticulously designed and synthesized using a room-temperature method for the enrichment of mycotoxins. The CX4-COF exhibited a porous crystalline network with an eclipsed AA-stacking configuration. The ingenious integration of NiFe 2 O 4 , supramolecular calix[4]arene and COF contributed to host-guest mediated recognition, size-selectivity and high adsorption capacity, rapidly reaching adsorption equilibrium within only 3 min. Simulation calculations revealed that the host-guest interaction, size effect and abundant binding sites facilitated synergistically recognize and capture mycotoxins. NiFe 2 O 4 @CX4-COF has successfully applied for simultaneous extraction and analysis of mycotoxins in cereals, achieving negligible matrix effects (−14% to 13%), high sensitivity (LODs of 0.003–0.014 μg/L) and satisfactory recoveries (74.4%–116%). This work provides a prospective platform for constructing tailored macrocycle-based COFs under mild conditions for precise recognition and accurate analysis of trace hazardous substances in food.
研究领域:
食品科学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Yao Xiao,Ru Zhao,Zaizhi Liu,Lin Zhang,Huiyan Gu,Fengjian Yang,Lei Yang,Tingting Liu
An improved method was investigated to obtain the essential oil from the roots and rhizomes of Asarum heterotropoides (RRAH) via homogenate-microwave-assisted hydrodistillation compare to conventional grinding process and the RRAH essential oil, methyleugenol, 3,4,5-trimethoxytoluene, and 3,5-dimethoxytoluene all had inhibitory effects on Fusarium species, with methyleugenol exhibiting the greatest inhibitory effect on them. We systematically explored some of the parameters that may affect the yield of essential oils. The maximum yield of the RRAH essential oil was 27.98 ± 1.4 mL/kg under the optimized conditions (22000 r/min homogenization speed, 3 min homogenization time, 460 W microwave irradiation power, 23 min microwave irradiation time and 23 mL/g liquid solid ratio). Methyleugenol was the main component of RRAH essential oil (41.13%) analysed by GC MS. Studies on antifungal activity suggest that methyleugenol has potential and broad application prospects in the direction of preservative-related fields such as slow-release microencapsulation in the future.
研究领域:
植物化学
·
药用植物学
·
真菌学
✓ 已验证
产品关联
DOI
高影响因子
Insect Science
·
2024
·
IF 2.9
Wen-Xuan Qi,Feng Liu,Fang-Fang Liu,Hai-Yan Ren,Bang-Xian Zhang,Xiao-Qiang Yu,Xiang-Jun Rao
Antimicrobial peptides (AMPs) are critical components of innate immunity in diverse organisms, including plants, vertebrates, and insects. This study identified and characterized a novel Lepidoptera-specific AMP, named lepidoptin, from the invasive pest Spodoptera frugiperda (Lepidoptera: Noctuidae). Lepidoptin is a 116-amino acid protein containing a signal peptide and a novel β -sandwich domain that is distinct from previously reported AMPs. Temporal and spatial expression analyses revealed a significant upregulation of the lepidoptin gene in vivo and in cultured SF9 cells in response to pathogens. Molecular docking analysis identified a specific binding cavity. Enzyme-linked immunosorbent assay and binding assays confirmed that lepidoptin can bind to pathogen-associated molecular patterns, bacteria, and fungi. Recombinant lepidoptin exhibited potent antibacterial activity by inducing bacterial agglutination, inhibiting bacterial growth, increasing bacterial membrane permeability, and preventing biofilm formation. Lepidoptin also showed antifungal activity against the entomopathogenic fungus Beauveria bassiana by inhibiting spore germination, increasing fungal cell permeability, and increasing reactive oxygen species. Injection of recombinant lepidoptin into S. frugiperda larvae increased survival after B. bassiana infection, whereas knockdown of lepidoptin by RNA interference decreased larval survival. In addition, lepidoptin showed antimicrobial activity against the plant pathogen Fusarium graminearum by inhibiting spore germination and alleviating disease symptoms in wheat seedlings and cherry tomatoes. This study demonstrates the remarkable dual functionality of lepidoptin in enhancing S. frugiperda immunity and controlling plant pathogens, making it a promising candidate for biocontrol strategies in both pest management and plant disease prevention.
研究领域:
昆虫学
·
微生物学
·
分子生物学
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植物病理学
·
害虫管理
·
生物防治
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）