Biomolecules
·
2020
·Vol. 10
·期 2
·231
·
IF 4.082
Rabia Durrani,Faez Iqbal Khan,Shahid Ali,Yonghua Wang,Bo Yang
Phospholipase B (EC 3.1.1.5) are a distinctive group of enzymes that catalyzes the hydrolysis of fatty acids esterified at thesn-1andsn-2positions forming free fatty acids and lysophospholipids. The structural information and catalytic mechanism of phospholipase B are still not clear. Herein, we reported a putative phospholipase B (TmPLB1) fromTalaromyces marneffeiGD-0079 synthesized by genome mining library. The gene (TmPlb1) was expressed and the TmPLB1 was purified usingE. colishuffle T7 expression system. The putative TmPLB1 was purified by affinity chromatography with a yield of 13.5%. The TmPLB1 showed optimum activity at 35 °C and pH 7.0. The TmPLB1 showed enzymatic activity using Lecithin (soybean > 98% pure), and the hydrolysis of TmPLB1 by31P NMR showed phosphatidylcholine (PC) as a major phospholipid along with lyso-phospholipids (1-LPC and 2-LPC) and some minor phospholipids. The molecular modeling studies indicate that its active site pocket contains Ser125, Asp183 and His215 as the catalytic triad. The structure dynamics and simulations results explained the conformational changes associated with different environmental conditions. This is the first report on biochemical characterization and structure dynamics of TmPLB1 enzyme. The present study could be helpful to utilize TmPLB1 in food industry for the determination of food components containing phosphorus. Additionally, such enzyme could also be useful in Industry for the modifications of phospholipids.Keywords:free fatty acids (FFAs);NMR (nuclear magnetic resonance);phospholipase B;Talaromyces marneffei;affinity chromatography
研究领域:
生物化学
·
分子生物学
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食品科学
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PubMed
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 12
·1141
·
IF 5.743
Chunyuan Tian,Minggang Wei,Xiaobin Wang,Qing Hua,Feiyan Tang,Lijun Zhao,Xuming Zhuang,Feng Luan
In the present study, the electrochemiluminescence (ECL) properties of Gd(OH)3nanocrystals with K2S2O8as the cathode coreactant were studied for the first time. Based on the prominent ECL behavior of this material and the excellent specificity of the aptamer technique, an ECL aptasensor for the detection of ochratoxin A (OTA) was formulated successfully. Over an OTA concentration range of 0.01 pg mL−1to 10 ng mL−1, the change in the ECL signal was highly linear with the OTA concentration, and the limit of detection (LOD) was 0.0027 pg mL−1. Finally, the ECL aptasensor was further used to detect OTA in real samples (grapes and corn) and satisfactory results were obtained, which indicated that the built method is expected to be applied in food detection.Keywords:Gd(OH)3nanocrystals;electrochemiluminescence;aptasensor;ochratoxin A;food detection
研究领域:
分析化学
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食品科学
·
毒理学
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PubMed
高影响因子
Nature Communications
·
2023
·Vol. 14
·期 1
·1-19
·
IF 16.6
Zhang Peisen,Cheng Junwei,Lu Yijie,Zhang Ni,Wu Xiaoai,Lin Hua,Li Wei,Wang Jian,Winnik Mitchell A.,Gan Zhihua,Hou Yi
Magnetic resonance (MR) angiography is one of the main diagnostic approaches for cardiac-cerebral vascular diseases. Nevertheless, the non-contrast-enhanced MR angiography suffers from its intrinsic problems derived from the blood flow-dependency, while the clinical Gd-chelating contrast agents are limited by their rapid vascular extravasation. Herein, we report a hypersensitive MR angiography strategy based on interlocking stratagem of zwitterionic Gd-chelate contrast agents (PAA-Gd). The longitudinal molar relaxivity of PAA-Gd was 4.6-times higher than that of individual Gd-chelates as well as appropriate blood half-life (73.8 min) and low immunogenicity, enabling sophisticated micro-vessels angiography with a resolution at the order of hundred micrometers. A series of animal models of cardiac-cerebrovascular diseases have been built for imaging studies on a 7.0 T MRI scanner, while the clinical translation potential of PAA-Gd has been evaluated on swine on a 3.0 T clinical MRI scanner. The current studies offer a promising strategy for precise diagnosis of vascular diseases.
研究领域:
医学影像
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血管疾病
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磁共振血管成像
·
造影剂
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PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Bo An,Qin Zhang,Ke Wang,Jiaqi Yang,Dongxing Song,Huijuan Su
The Gd 0.1 Ce 0.9- x Sr x O 2-δ ( x = 0, 0.01, 0.04, 0.07) electrolytes were synthesized via the citric acid-EDTA combustion method as electrolyte materials for intermediate-temperature solid oxide fuel cells (IT-SOFCs), aiming to enhance ionic conductivity and mechanical stability of SOFCs at intermediate-to-high temperatures. Sr²⁺ doping improved electrolyte densification, increased grain volume, and reduced grain boundary density, thereby enhancing the dielectric constant. These modifications decreased both intragrain and grain boundary resistances and facilitated oxygen ion migration. At 500°C, the optimized Gd 0.1 Ce 0.86 Sr 0.04 O 2-δ exhibited a 6.4-fold increase in ionic conductivity and a 50% reduction in activation energy compared to Gd 0.1 Ce 0.9 O 1.95 . This enhancement was partially attributed to the improved scavenging coefficient of Gd 0.1 Ce 0.86 Sr 0.04 O 2-δ , whose blocking factor decreased by approximately 43% relative to Gd 0.1 Ce 0.9 O 1.95 . Dielectric property analysis elucidated the contribution of Gd-Sr synergistic effects to interfacial polarization.In terms of mechanical properties, the 4GSDC sample demonstrated a 13.5% increase in Vickers hardness, 69.7% improvement in fracture toughness, and 24.91% enhancement in flexural strength compared to GDC. At 750°C, single cells with the 4GSDC electrolyte achieved a power density of 1100 mW/cm², representing a 55.4% improvement over GDC. Density functional theory (DFT) calculations revealed that Sr²⁺ incorporation effectively reduced binding energy and migration barriers. Therefore, Sr-Gd co-doping can be regarded as an effective strategy to enhance the performance of ceria-based electrolytes in IT-SOFCs.
研究领域:
材料科学
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能源技术
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电化学
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固体氧化物燃料电池
·
陶瓷材料
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高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2025
·Vol. 109
·期 1
·e70441
·
IF 3.8
Yun Wang,Sihan Yang,Xuejiao Wang,Qi Zhu,Ji-Guang Li
The growing demand for near-infrared phosphor-converted light-emitting diodes (NIR pc-LEDs) has posed significant challenges to the development of NIR-emitting phosphors with high quantum efficiency and thermal stability. A series of (Gd 3- x Ca x )(Hf x Sc y Al 3 Cr 0.05 )O 12 ( x + y = 1.95, x = 0.45–1.95, y = 0.0–1.5) garnet phosphors, designed by modifying both the Gd and Al sites of Gd 3 Al 5 O 12 , were fabricated by solid-state reaction and investigated for their structure and NIR luminescence. Ca 2+ and Hf 4+ /Sc 3+ /Cr 3+ would replace the Gd 3+ in [GdO 8 ] dodecahedron and the Al in [AlO 6 ] octahedron, respectively. The phosphors are effectively excitable by 452 nm blue light to emit NIR light, and a higher Ca 2+ /Hf 4+ content ( x value) would red-shift (from ∼732 to 750 nm) and broaden (full width at half maximum from ∼82.7 to 87.6 nm) the emission through weakening the crystal field of Cr 3+ . The x = 0.95 optimal phosphor was determined to have external/internal quantum efficiencies (%) of 26.5/54.9 and a thermal stability of 81.6%@423 K. The NIR pc-LED device based on the x = 0.95 phosphor achieved an output power of 34.71 mW and a photoelectric conversion efficiency of 12.01% at a driving current of 100 mA and showed the potential for non-destructive testing, night vision, and medical imaging.
研究领域:
材料科学
·
光学材料
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固态照明
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光子学
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发光材料
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高影响因子
CHINESE CHEMICAL LETTERS
·
2014
·Vol. 25
·期 6
·892-896
·
IF 1.178
Ting-Ting Dai,Lei Liu,Dong-Liang Tao,Shi-Gang Li,Hong Zhang,Yu-Min Cui,Yong-Zhong Wang,Ji-Tang Chen,Kun Zhang,Wen-Zhong Sun,Xiao-Yun Zhao
Absolute quantum yield ( Φ ) is one of the most important parameters to evaluate the potential of novel materials. Lanthanide complexes Eu x Gd 1− x (TTA) 3 phens are synthesized with the ratio of Gd 3+ dopant concentration ranging from 10% to 90% to improve the absolute quantum yield. Eu x Gd 1− x (TTA) 3 phens possess similar infrared and ultraviolet spectra, showing that they have similar molecular structures. The absolute emission quantum yields of Eu x Gd 1− x (TTA) 3 phens are determined using a fluoromax-4 spectrofluorometer equipped with an integrating sphere. The fluorescence lifetimes of the Eu x Gd 1− x (TTA) 3 phens are measured in the same experiment. It was found that both absolute quantum yields and fluorescence lifetimes of Eu x Gd 1− x (TTA) 3 phens are of quasi-periodic variation with the change of the Gd 3+ dopant concentrations. The absolute quantum efficiency and fluorescence lifetime vary with respect to the Gd content in an opposite fashion, indicating that the rate of energy absorption by the Eu x Gd 1− x (TTA) 3 phens and the conversion to light energy is critical for the absolute quantum efficiency. The radiative rate constant K r and non-radiative rate constant K nr are calculated. The dependence of K r and K nr on the Gd 3+ dopant concentrations is very similar to that of absolute quantum efficiency. The radiation rate constant K r and absolute quantum efficiency have a linear relationship.
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高影响因子
DALTON TRANSACTIONS
·
2016
·Vol. 45
·期 13
·5800-5807
·
IF 4.177
Ai-Hua Li,Mengyun Lü,Jun Yang,Lin Chen,Xiaohong Cui,Zhijun Sun
Sub-20 nm core–shell and water-soluble SrF2:Yb,Tm@CaF2:Gd heteronanoparticles with both upconversion luminescence (UCL) and magnetic resonance imaging (MRI) capabilities were designed and synthesized via a two-step hydrothermal method. In the design of the heteronanoparticles, SrF2:Yb,Tm nanoparticles with high UCL efficiency are chosen as the core material for strong UCL output; and by epitaxially coating the SrF2:Yb,Tm core particles with inert and biocompatible shells of CaF2:Gd, the core–shell heteronanoparticles are endowed with a magnetic capability (longitudinal relaxivity of 2.4 mM−1 s−1) for MRI, as well as an enhancement of the near infrared (NIR) UCL by 9.2 times. The aqueous dispersion of SrF2:Yb,Tm@CaF2:Gd heteronanoparticles with a concentration of 2.6 wt% can emit NIR UCL so as to be easily detected with a fiber optical spectrometer under illumination of a 975 nm laser diode with a power density of 8.8 W cm−2. Such a dispersion with a Gd3+ concentration of 0.0143 mM in the shell region of the heteronanoparticles can also generate the detectable quickening of longitudinal relaxation. The results promise the strong potential of this nanomaterial for applications in bioimaging as a dual-functional probe.
研究领域:
纳米技术
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生物医学成像
·
材料科学
·
分子诊断
·
诊疗一体化
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DOI
高影响因子
LANGMUIR
·
2026
·
IF 4.4
Youli Zhang,Lulu Wang,Yanmin Zheng,Lei Zhu,Dongsheng Li,Xinwei Zheng
Balancing the thermodynamic stability and biological clearance of supramolecular assemblies represents a fundamental challenge in the design of functional nanomedicines. To address this, we propose a concentration-responsive “assemble-for-uptake, disassemble-for-clearance” strategy implemented via rational molecular engineering of a minimalist, single-component Gd–DOTA amphiphile. We demonstrate that the alkyl chain length acts as a precise molecular dial to tune the critical micelle concentration (CMC), thereby dictating micellar stability, biological identity, and clearance pathways. Through this tuning, we identified Gd–C12 as the optimized agent, which self-assembles into spherical nanomicelles (38.6 ± 8.3 nm) with desirable T1 contrast (r2/r1 < 2) and kinetic stability surpassing unmodified Gd–DOTA. In vivo studies reveal that Gd–C12 provides potent hepatic enhancement (120% increase) via favorable MPS recognition, independent of organic anion-transporting polypeptide (OATP). Crucially, following hepatic uptake, the agent undergoes spontaneous disassembly upon physiological dilution/excretion, facilitating rapid systemic clearance (>97%) to resolve long-term retention concerns. This work presents Gd–C12 as a high-performance, transporter-independent contrast agent that bridges the gap between nanoparticles and small molecules, holding promise for providing reliable hepatic parenchymal enhancement regardless of OATP expression levels.
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高影响因子
JOURNAL OF RARE EARTHS
·
2024
·
IF 5.2
Jun Wang,Jianyu Li,Chunyu Jiang,Jianbo Song,Xing Yang,Xiaolong Ma,Kailiang Luo,Peng Wu,Xiaoyu Chong,Jing Feng
Thermal barrier coatings (TBCs) materials with lowered thermal and oxygen ion conductivity can provide thermal and oxidative protection for high temperature hot-end components in aeronautical engines and gas turbines . The rare-earth tantalate RETaO 4 (RE = Dy, Gd and Sm) ceramics with monoclinic (m) phase were successfully synthesized via spark plasma sintering . Oxygen vacancies responsible for the thermal and oxygen ion conductivities of RETaO 4 were demonstrated by atomic-resolution energy dispersive X-ray and X-ray photoelectron spectroscopy . Among the three samples, DyTaO 4 has excellent oxygen/thermal barrier performance. Compared to the current service thermal barrier coating material ZrO 2 -8 wt% Y 2 O 3 (8 YSZ), DyTaO 4 has an ultra-low oxygen ion conductivity benefiting from low oxygen vacancy concentration and strong stretching force constants. The intrinsic thermal conductivity of DyTaO 4 is 68.2% less than that of 8 YSZ . Additionally, the thermal expansion rate curves indicate that the phase transformation does not happen from room temperature to 1200 °C. The above results demonstrate that high-growth rate thermally grown oxide can be retarded by creating dense DyTaO 4 coating with lowered thermal and oxygen ion conductivity.
研究领域:
材料科学
·
航空工程
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高影响因子
AAPS PHARMSCITECH
·
2025
·Vol. 26
·期 5
·1-13
·
IF 4
Yang Guixiang,Zhang Yi,Li Chong,Xia Xingle,Jin Qian,Li Liqin,Zong Xingxing,Liu Dongxin,Wei Jingchen,Chen Xuejun,Wang Chen
Huperzine A (Hup A), a reversible acetylcholinesterase inhibitor, shows protective potential against neurotoxic poisoning. Current formulations (oral tablets/capsules and injectables) face limitations: oral administration suffers from first-pass metabolism and fluctuating plasma concentrations due to frequent dosing, while injectables require sterile administration, reducing long-term compliance. The aim of this study was to prepare dissolving microneedles (MNs) patches containing Hup A for pretreatment against soman (GD) poisoning. Hyaluronic acid (HA), polyvinylpyrrolidone K30 (PVP K30) and sorbitol were selected as matrix materials in specific proportions and concentration ranges based on single-factor screening experiments and orthogonal design optimization. In order to further evaluate the potential of the Hup A MNs, optimized formulations were selected for subjected to characterization. This evaluation encompassed morphological observation, mechanical properties, skin penetration efficacy, recovery time, in vitro drug release study, permeability assays, pharmacokinetic study, and pharmacodynamic study. Statistical analyses were performed using Student's t-tests for pairwise comparisons and one-way ANOVA for multiple comparisons. The results demonstrated that prepared Hup A MNs had a conical shape with a smooth surface and sharp tips. Hup A MNs had sufficient mechanical strength (122.3 ± 15.3 N) to penetrate through the skin and could be quickly skin of recovery 6 min. In permeability assays, the cumulative Hup A release was about 35.4 μg at 2 h and 58 μg at 10 h. Pharmacodynamic results demonstrated that compared to oral administration, Hup A MNs reduced the onset time (30 min vs . 60 min) and extended the effective prevention duration against GD poisoning (6 h vs . 2 h). Pharmacokinetic analysis showed that MNS administration can maintain a more lasting and stable blood concentration as compared to oral administration. MNs are a valuable drug delivery system, benefiting the patients with minimal skin invasion. Taken together, these results demonstrated that Hup A-loaded MNs are acquiring a new alternative for pretreatment of OPNAs. Graphical
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PubMed
高影响因子
CHEMICAL & PHARMACEUTICAL BULLETIN
·
2026
·Vol. 74
·期 3
·232-239
·
IF 1.5
Yuhuan Zhao,Yuhe Yang,Xiaorui Ru,Xiaobo Wang,Chunxi Li,Jiangyan Tang,Yun Zhang,Sanhu Gou,Hui Liu,Jingman Ni
To enhance the efficacy of magnetic resonance imaging (MRI), numerous novel contrast agents have been developed, with peptide modification emerging as a promising strategy. The versatility of peptides renders them particularly useful molecules for the fabrication of new molecule. However, the influence of peptide side chain modification on the imaging performance of contrast agents has not been fully explored in existing studies. Herein, we employed XK (ε-DOTA-Gd) X as a Gd-DOTA-peptide template and the X molecule underwent amino acid block substitution. Six Gd-DOTA-peptide complexes were synthesized and their properties, such as relaxivity, biostability, and biocompatibility, were measured and in vitro MRI assays were carried out. The results demonstrated that the Gd-DOTA-peptides exhibited low hemolysis rates and cytotoxicity. In plasma, the half-lives of the Gd-DOTA-peptide contrast agents exceeded 5 h. Relaxation experiments revealed that the Gd-DOTA-peptide achieved a slightly higher relaxation rate compared with the commercial agent Dotarem. Therefore, the amino acid modularize modification on the gadolinium-based MRI contrast agents were effective and worthy of further investigation.
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PubMed
高影响因子
INORGANIC CHEMISTRY
·
2016
·
IF 4.82
Dong Yan,Fei-Fei Mao,Jiang-Gao Mao
A series of lanthanide boroantimonates, namely, LnBSb2O8 (Ln = Sm 1, Eu 2, Gd 3, and Tb 4) have been successfully synthesized by high temperature solid-state reactions for the first time. They are isostructural and feature novel three-dimensional (3D) frameworks composed of 2D [Sb3O12]9– layers interconnected by 1D [SbBO7]6– chains with remaining BO3 groups hanging on the walls of the 1D 6-membered-ring (MR) tunnels along the a-axis, and the lanthanide ions filled in the voids of the anionic structure. They exhibit high thermal stability (up to 900 °C). Luminescent studies suggest that compounds 1, 2, and 4 have potential application as orange, red, and green light luminescent materials, respectively. Magnetic measurements reveal ferromagnetic coupling interactions in compound 3 and antiferromagnetic coupling interactions between magnetic centers in compounds 1, 2, and 4.
研究领域:
无机化学
·
材料科学
·
固态化学
·
发光学
·
磁学
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DOI
高影响因子
危险品化学品经营许可证（带存储）