MICROCHEMICAL JOURNAL
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2026
·
IF 5.2
Keyan Wei,Chang-ye Hui,Jinwen Ling,Wenjun Luo,Jiaqiong Xiong,Yuting Chen,Yan Guo
A single-plasmid dual-color biosensor for As(III) and Hg(II) visual detection. • Detection limits near or below drinking water regulatory thresholds. • Independent blue and purple pigments enable direct color discrimination. • Validated for environmental water matrices including seawater samples. A novel dual-color whole-cell biosensor was engineered for simultaneous visual detection of As(III) and Hg(II) within a single microbial host. The biosensor integrates an As(III)-responsive ArsR module driving indigo biosynthesis and an Hg(II)-responsive MerR module driving deoxyviolacein (DV) biosynthesis into a single plasmid designated pAs-indigo-Hg-DV. Upon exposure, the engineered Escherichia coli TOP10 generates distinct blue and purple pigments with absorption maxima at 620 nm and 570 nm, respectively. The system achieved limits of detection of 0.39 μM for As(III) (linear range 0.39–6.25 μM) and 0.49 nM for Hg(II) (linear range 7.8–125 nM), both approaching or falling below the respective drinking water regulatory limits. By using two independent color outputs from a single host, a dual-color biosensor for microscopic differential detection of two toxic metals was achieved, addressing the key unmet need of a flexible multi-channel multiplexed whole-cell biosensing system. The sensor supports naked-eye preliminary screening in a standard 96-well microplate format without dependence on instrumentation. It exhibited excellent Hg(II) selectivity and maintained robust performance in surface water and seawater matrices. This pigment-based dual-target strategy offers a low-cost, high-throughput platform for rapid co-exposure assessment of toxic heavy metals, providing a practical complement to conventional instrumental analysis and advancing the development of innovative biosensors for environmental monitoring. Download: Download high-res image (164KB) Download: Download full-size image
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DESALINATION
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2026
·
IF 9.9
Ming Li,Xiaofei Fan,Jianing Hu,Jinghong Zhang,Huining Xiao,Yuling Zhang
A 3D porous MXene/cellulose aerogel is fabricated by sol-gel and freeze-drying technique. • It achieves a high Hg(II) uptake of 877.19 mg/g at 318 K via chemisorption and redox. • The aerogel also removes methylene blue efficiently by electrostatic attraction. • It shows good reusability, easy separation, and structural stability. Aqueous contamination by heavy metal ions and organic dyes represents two major categories of water pollutants. Developing adsorbents that can efficiently remove both types is highly desirable. Here, multilayered MXene (Ti 3 C 2 T x ) was prepared by selective etching of Ti 3 AlC 2 using LiF/HCl and combined with cellulose to construct a three-dimensional porous MX@Cell composite aerogel via sol-gel and freeze-drying. The materials were characterized by SEM, XRD, FTIR, nitrogen adsorption-desorption, and XPS. Batch adsorption tests for Hg(II) and MB were conducted separately. MX@Cell-25 (25 wt% MXene) achieved a maximum theoretical Hg(II) capacity of 877.19 mg/g at 318 K, outperforming numerous previously reported adsorbents. It followed pseudo-second-order kinetics and the Freundlich isotherm, indicating chemisorption-dominated multilayer adsorption on a heterogeneous surface. Thermodynamic analysis indicated that the adsorption of Hg(II) on MX@Cell-25 was a spontaneous process accompanied by an entropy increase and an endothermic reaction ( ΔH > 0), confirming that higher temperatures favored the adsorption process. XPS revealed a synergistic mechanism of electrostatic attraction, surface coordination, and redox reactions. For MB, the maximum capacity was 104.93 mg/g at 318 K, with pseudo-second-order kinetics and Langmuir isotherm fitting, suggesting monolayer adsorption driven by electrostatic attraction. The composite showed good reusability (about 85% Hg(II) capacity retention after seven cycles), as well as easy separability and environmental friendliness. This work demonstrates that MXene/cellulose composite aerogel is a promising candidate for removing both heavy metal ions and organic dyes from water. Download: Download high-res image (274KB) Download: Download full-size image
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RSC Advances
·
2025
·Vol. 15
·期 45
·38069-38081
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IF 4.6
Mingmin Bai,Wenyuan Guo,Yiyang Zhang,Ruiqiang Yang,Weixin Li
A novel multifunctional carbon-modified MoS2 material (C-W-D-MoS2-x) was synthesized through a solvothermal method, exhibiting expanded interlayer spacing, a large surface area (∼47.03 m2 g−1), and abundant exposed sulfur (S) active sites that enabled efficient adsorption of Hg2+ from wastewater. Among the prepared variants, C-W-D-MoS2-0.03 showed the highest adsorption performance, achieving an exceptional distribution coefficient (Kd) of 2.0 × 105 mL g−1. The adsorption kinetics were best described by the pseudo-second-order model, while the adsorption isotherms were well-fitted to the Langmuir model, with a maximum adsorption capacity (qm) of 1974.0 mg g−1. This remarkable adsorption capability of C-W-D-MoS2-0.03 can be attributed to the synergistic effect of carbon functional groups and the high density of S active sites. Furthermore, an alumina inorganic membrane functionalized with C-W-D-MoS2-0.03 was successfully assembled into a device, demonstrating a dynamic removal process that reduced 50 mg L−1 of Hg2+ to below 0.1 mg L−1.
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SENSORS AND ACTUATORS B-CHEMICAL
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2015
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IF 4.097
Wenge Yang,Shouhai Yang,Qirun Guo,Tuan Zhang,Keyi Wu,Yonghong Hu
A phenothiazine–aminothiourea-based open chemosensor ( P1 ) has been designed and synthesized, which exhibits high selectivity toward Hg 2+ by forming a 1:1 complex. In its Hg 2+ ensemble, it is reported as a highly sensitive and selective probe for the detection of iodide based on the displacement approach in aqueous solution. The addition of iodide to a solution of phenothiazine–aminothiourea–Hg 2+ ensemble resulted in a rapid and remarkable fluorescence off–on (emission at 565 nm). The practical use of an ‘ensemble’ was demonstrated by its application to the detection of iodide in the living cells.
研究领域:
分析化学
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生物医学
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材料科学
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MICROCHIMICA ACTA
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2018
·Vol. 185
·期 5
·1-9
·
IF 5.705
Han Xiujuan,Meng Zuchao,Zhang Hongfang,Zheng Jianbin
Various carbon nanomaterials for use in anodic stripping voltammetric analysis of Hg(II), Cu(II), Pb(II) and Cd(II) are screened. Graphene, carbon nanotubes, carbon nanofibers and fullerene (C 60 ), dispersed in chitosan (Chit) aqueous solution, are used to modify a glassy carbon electrode (GCE). The fullerene-chitosan modified GCE (C 60 -Chit/GCE) displays superior performance in terms of simultaneous determination of the above ions. The electrodes and materials are characterized by electrochemical impedance spectroscopy, cyclic voltammetry, scanning electron microscopy, Raman spectroscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The excellent performance of C 60 -Chit/GCE is attributed to the good electrical conductivity, large surface area, strong adsorption affinity and unique crystalline structure of C 60 . Using differential pulse anodic stripping voltammetry, the assay has the following features for Hg(II), Cu(II), Pb(II) and Cd(II), respectively: (a) Peak voltages of +0.14, −0.11, −0.58 and − 0.82 V (vs SCE); (b) linear ranges extending from 0.01–6.0 μM, 0.05–6.0 μM, 0.005–6.0 μM and 0.5–9.0 μM; and (c), detection limits (3σ method) of 3 nM (0.6 ppb), 14 nM (0.9 ppb), 1 nM (0.2 ppb) and 21 nM (2.4 ppb). Moreover, the modified GCE is well reproducible and suitable for long-term usage. The method was successfully applied to the simultaneous determination of these ions in spiked foodstuff.
Compared with graphene, carbon nanotubes and carbon nanofibers, an electrode modified with fullerene in chitosan electrode displays superior performance for the simultaneous anodic stripping voltammetric detection of Hg(II), Cu(II), Pb(II) and Cd(II).
研究领域:
分析化学
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环境科学
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食品科学
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高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
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IF 4
Jiangxiong Zhu,Hao Yin,Yidong Xin,Lumei Wang,Xueqing Geng,Haiyun Zhao,Yun Deng
A novel structure-switching “turn-on” fluorometric aptasensor was developed for simultaneous detection of Hg(II) and Pb(II) in grapes, broccoli, and cabbage. The sensing mechanism utilizes fluorescence resonance energy transfer (FRET) between a FAM-labeled aptamer (FAM-Apt) and a BHQ1-labeled complementary strand (CS-BHQ1). In the absence of target ions, FAM-Apt and CS- BHQ1 bind through complementary base pairing, bringing FAM and BHQ1 into close proximity, which induces FRET and quenches FAM’s fluorescence. Target ion binding induces a conformational change in the FAM-Apt, disrupting FRET and restoring fluorescence. Optimized parameters include reagent addition sequence, Apt and CS concentrations, incubation times, buffer conditions, and salt concentrations. Under optimized conditions, the aptasensor achieved limits of detection (LODs) of 2.29 μg/L for Hg(II) and 0.24 μg/L for Pb(II), much lower than the regulatory limits for fruits and vegetables, and the limits of quantification (LOQ) for food samples were 6.02 μg/kg and 2.07 μg/kg, respectively. Furthermore, the constructed aptasensor could rapidly complete detection within 22.5 min and demonstrated high selectivity against interfering ions. Spiked sample recoveries ranged from 98.07-104.12% for Hg(II) and 89.23-105.46% for Pb(II), with relative standard deviations of 0.95-5.64% and 1.21-14.53%, respectively. This aptasensor provides a sensitive, rapid, and cost-effective alternative to conventional detection methods.
研究领域:
分析化学
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环境科学
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食品科学
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Polymers
·
2019
·Vol. 11
·期 5
·815
·
IF 3.164
Mihray Abdulla,Ahmat Ali,Ruxangul Jamal,Tursunnisahan Bakri,Wei Wu,Tursun Abdiryim
Heavy metal ions in water, cosmetics, and arable land have become a world-wide issue as they cause a variety of diseases and even death to humans and animals when a certain level is exceeded. Therefore, it is necessary to development a new kind of sensor material for the determination of heavy metal ions. In this paper, we present an electrochemical sensor based on composite material (thiol(–SH) grafted poly(3,4-proplenedioxythiophene) (PProDOT(MeSH)2)/ porous silicon spheres (Si) composite, denoted as PProDOT(MeSH)2@Si) from the incorporation of thiol(–SH) grafted poly(3,4-proplenedioxythiophene) (PProDOT(MeSH)2) with porous silicon spheres (Si) for the electrochemical detection of heavy metal ions (Cd(II), Pb(II), and Hg(II)). The PProDOT(MeSH)2@Si composite was synthesized via a chemical oxidative polymerization method. The structure and morphology of PProDOT(MeSH)2@Si composite were characterized by Fourier transform infrared (FT-IR), Ultraviolet–visible spectroscopy (UV–Vis), X-ray diffraction (XRD), scanning electron microscope (SEM), Transmission electron microscope (TEM), and Brunauer−Emmett−Teller (BET). Furthermore, the electrochemical performance of PProDOT(MeSH)2@Si was evaluated by detecting of Cd(II), Pb(II), and Hg(II) ions using the differential pulse voltammetry (DPV) method. The relationship between structural properties and the electrochemical performance was systematically studied. The results showed that the entry of two thiol-based chains to the monomer unit resulted in an increase in electrochemical sensitivity in PProDOT(MeSH)2,which was related to the interaction between thiol group(-SH) and heavy metal ions. And, the combination of PProDOT(MeSH)2with Si could improve the electrocatalytic efficiency of the electrode material. The PProDOT(MeSH)2@Si/GCE exhibited high selectivity and sensitivity in the rage of 0.04 to 2.8, 0.024 to 2.8, and 0.16 to 3.2 μM with the detection limit of 0.00575, 0.0027, and 0.0017 µM toward Cd(II), Pb(II), and Hg(II), respectively. The interference studies demonstrated that the PProDOT(MeSH)2@Si/GCE possessed a low mutual interference and high selectivity for simultaneous detection of Cd(II), Pb(II), and Hg(II) ions.Keywords:thiol(–SH), poly(3,4-proplenedioxythiophene), porous silicon spheres;electrochemical sensor;heavy metal ions;simultaneous detection
研究领域:
材料科学
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环境科学
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分析化学
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高影响因子
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
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2019
·
IF 1.267
Chunlai Wu,Chuanyun Peng
To develop an accurate and precise method for separation and pre-concentration of Hg(II), a novel thionin functionalised core shell structure magnetic material has been prepared and characterised. The extraction ability of the material was evaluated by magnetic solid-phase extraction coupled with inductively coupled plasma mass spectrometry determination of Hg(II) in food and water samples. Combining the advantages of magnetic separation with selective extraction of thionin towards Hg(II), the material exhibits enhanced enrich selectivity and efficiency for Hg(II). The experimental parameters influencing Hg(II) extraction efficiency, including pH of the aqueous solution, the dosage of the adsorbent, extraction time and sample volume, were systematically investigated. Under the optimised conditions, concentration of Hg(II) at 1.0 μg L−1 can be successfully enriched by the material without the interference of the common co-existing ions. The enrichment factor and adsorption capacity were 250 and 75.2 mg g−1, and precise of the method was confirmed by analysing the spiked food, water samples and standard water reference samples with the recoveries of 92.5–101.8%.
研究领域:
分析化学
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环境科学
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食品科学
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PLASMA CHEMISTRY AND PLASMA PROCESSING
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2016
·Vol. 36
·期 4
·1051-1065
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IF 1.811
Peng D. L.,Ji H. F.,Dong X. D.,Tian J. F.,Wang M. H.,He L. H.,Zhang Z. Z.,Fang S. M.
In this study, a nanocomposite consisting of three-dimensional reduced graphene oxide (3D-rGO) and plasma-polymerized propargylamine (3D-rGO@PpPG) was prepared and used as a highly sensitive and selective DNA sensor for detecting Hg 2+ . Given the high density of amino groups in the resultant 3D-rGO@PpPG nanocomposite, thymine-rich and Hg 2+ -targeted DNA was preferentially immobilized on the fabricated sensor surface via the strong electrostatic interaction between DNA strands and the amino-functionalized nanocomposites, followed by detecting Hg 2+ through T–Hg 2+ –T coordination chemistry between DNA and Hg 2+ . The results of electrochemical measurements revealed that the anchored amount of DNA strands anchored on the 3D-rGO@PpPG nanofilm surface affects the determination of Hg 2+ in aqueous solution. It showed high sensitivity and selectivity toward Hg 2+ within concentrations ranging from 0.1 to 200 nM and displayed a low detection limit of 0.02 nM. The new strategy proposed also provides high selectivity of Hg 2+ against other interfering metal ions, good stability, and repeatability. The excellent applicability of the developed sensor confirms the potential use of plasma-modified nanofilms for the detection of heavy metal ions in real environmental samples and water.
研究领域:
環境科學
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分析化學
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納米技術
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生物技術
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高影响因子
SEPARATION SCIENCE AND TECHNOLOGY
·
2016
·
IF 1.083
Maosheng Deng,Ying Huang,Xin Zhang,Zhijun Feng,Jinfeng Gou,Binjie Sun
A novel chelating resin bearing amidinothiourea (ADTU) moieties was prepared by chloroethyl crosslinked polystyrene (CMPS) with ADTU in the presence of a phase transfer catalyst. Fourier transform infrared spectra (FT-IR) and scanning electron microscopy (SEM) were used to characterized the resulting chelating resin. The saturated adsorption capacity of Hg(II) ions estimated from the Langmuir model was up to 1215 mg g−1at 313 K. Furthermore, the resin was able to selectively separate Hg(II) from multicomponent solutions with Cd(II), Co(II), Cu(II), Zn(II), Mg(II) and Pb(II). The desorption process showed the mercury recovery reached to 99.2% under eluting condition of 1 M HCl + 8% thiourea.
研究领域:
环境科学
·
材料科学
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高影响因子
RSC Advances
·
2022
·Vol. 12
·期 51
·33160-33174
·
IF 4.036
Yu Zhou,Zheng Zeng,Yongfu Guo,Xinyu Zheng
A novel diatomite-based mesoporous material of MCM-41/co-(PPy-Tp) was prepared with MCM-41 as carrier and functionalized with the copolymer of pyrrole and thiophene. The physicochemical characteristics of the as-prepared materials were characterized by various characterization means. The removal behaviour of Hg(II) was adequately investigated via series of single factor experiments and some vital influence factors were optimized via response surface methodology method. The results exhibit that diatomite-based materials MCM-41/co-(PPy-Tp) has an optimal adsorption capability of 537.15 mg g−1 towards Hg(II) at pH = 7.1. The removal process of Hg(II) onto MCM-41/co-(PPy-Tp) is controlled by monolayer chemisorption based on the fitting results of pseudo-second-order kinetic and Langmuir models. In addition, the adsorption of Hg(II) ions onto MCM-41/co-(PPy-Tp) is mainly completed through forming a stable complex with N or S atoms in MCM-41/co-(PPy-Tp) by electrostatic attraction and chelation. The as-developed MCM-41/co-(PPy-Tp) displays excellent recyclability and stabilization, has obviously selective adsorption for Hg(II) in the treatment of actual electroplating wastewater. Diatomite-based mesoporous material functionalized by the copolymer of pyrrole and thiophene exhibits promising application prospect.
研究领域:
环境科学
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材料科学
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TALANTA
·
2021
·
IF 6.057
Wenjia Li,Dong Liu,Daming Dong,Tianyan You
The rapid and sensitive detection of Hg 2+ is highly required to protect the environmental safety and human healthy. In the present work, a ratiometric fluorescent sensing platform, consisting of silicon quantum dots (SiQDs), Rox-labelled DNA (Rox-DNA), and Exonuclease III (Exo III), is developed for the accurate detection of Hg 2+ . As for fluorescent probe , we report the first use of glutathione as reduction reagent for the microwave synthesis of SiQDs, achieving the facile (using a house-hold microwave oven) and rapid (within 8 min) synthesis. Such SiQDs show pH-independent spectra and reversible fluorescent behavior with temperature. Moreover, experimental results revealed that the electrostatic interaction-induced aggregation of Rox-DNA and SiQDs facilitated the occurring of energy transfer (ET). And detection principle based on the regulation of ET between Rox and SiQDs with Exo III was designed for analysis. ET effect resulted in the fluorescent fading of Rox while that of SiQDs kept stable. For analysis, the addition of Hg 2+ led to the formation of double-stranded Rox-DNA via T-Hg 2+ -T. Exo III would cut these double-stranded DNA to release Rox and Hg 2+ , thereby impeding the ET effect and recovering the fluorescent of Rox. Such SiQDs/Rox-DNA/Exo III ratiometric fluorescent sensing platform exhibited a linear response concentration range of 0.02 nM–10 nM with a detection limit of 0.01 nM. It was successfully used to analyze the water and soil samples. The reliability was validated by ICP-MS. Our work should promote the practical application of ratiometric fluorescent assay.
研究领域:
分析化学
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环境科学
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生物医学工程
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高影响因子
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